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PMC:2222968 / 39453-39911 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T19788 0-3 CD denotes 3.5
T19789 4-6 NN denotes μg
T19790 7-9 IN denotes of
T19791 10-13 DT denotes the
T19792 14-19 NN denotes FOXP3
T19793 20-28 NN denotes promoter
T19794 29-39 NN denotes luciferase
T19795 40-48 NN denotes reporter
T19796 49-55 NN denotes vector
T19797 56-59 CC denotes and
T19798 60-63 CD denotes 0.5
T19799 64-66 NN denotes μg
T19800 67-74 NN denotes phRL-TK
T19801 75-79 VB denotes were
T19802 80-85 VB denotes added
T19803 86-88 TO denotes to
T19804 89-90 CD denotes 3
T19805 91-92 SYM denotes ×
T19806 93-96 CD denotes 106
T19807 97-101 JJ denotes CD4+
T19808 102-103 NN denotes T
T19809 104-109 NN denotes cells
T19810 110-121 VB denotes resuspended
T19811 122-124 IN denotes in
T19812 125-128 CD denotes 100
T19813 129-131 NN denotes μL
T19814 132-134 IN denotes of
T19815 135-147 NN denotes Nucleofector
T19816 148-156 NN denotes solution
T19817 157-158 -LRB- denotes (
T19818 158-163 NNP denotes Amaxa
T19819 164-174 NNP denotes Biosystems
T19820 174-175 -RRB- denotes )
T19821 176-179 CC denotes and
T19822 180-194 VB denotes electroporated
T19823 195-200 VB denotes using
T19824 201-204 DT denotes the
T19825 205-209 NN denotes U-15
T19826 210-217 NN denotes program
T19827 218-220 IN denotes of
T19828 221-224 DT denotes the
T19829 225-238 NNP denotes Nucleofector.
T19830 239-244 IN denotes After
T19831 245-246 DT denotes a
T19832 247-251 JJ denotes 24-h
T19833 252-259 NN denotes culture
T19834 260-262 IN denotes in
T19835 263-273 JJ denotes serum-free
T19836 274-284 NN denotes conditions
T19837 285-288 CC denotes and
T19838 289-296 NN denotes stimuli
T19839 297-299 IN denotes as
T19840 300-309 VB denotes indicated
T19841 310-312 IN denotes in
T19842 313-316 DT denotes the
T19843 317-324 NN denotes figures
T19844 324-325 -COMMA- denotes ,
T19845 326-336 NN denotes luciferase
T19846 337-345 NN denotes activity
T19847 346-349 VB denotes was
T19848 350-358 VB denotes measured
T19849 359-361 IN denotes by
T19850 362-365 DT denotes the
T19851 366-370 JJ denotes dual
T19852 371-381 NN denotes luciferase
T19853 382-387 NN denotes assay
T19854 388-394 NN denotes system
T19855 395-396 -LRB- denotes (
T19856 396-403 NNP denotes Promega
T19857 404-411 NNP denotes Biotech
T19858 411-412 -RRB- denotes )
T19859 413-422 VB denotes according
T19860 423-425 TO denotes to
T19861 426-429 DT denotes the
T19862 430-442 NN denotes manufacturer
T19863 442-444 POS denotes 's
T19864 445-457 NN denotes instructions
R14812 T19789 T19788 arg1Of μg,3.5
R14813 T19789 T19790 arg1Of μg,of
R14814 T19789 T19797 arg1Of μg,and
R14815 T19796 T19790 arg2Of vector,of
R14816 T19796 T19791 arg1Of vector,the
R14817 T19796 T19792 arg1Of vector,FOXP3
R14818 T19796 T19793 arg1Of vector,promoter
R14819 T19796 T19794 arg1Of vector,luciferase
R14820 T19796 T19795 arg1Of vector,reporter
R14821 T19797 T19801 arg1Of and,were
R14822 T19797 T19802 arg2Of and,added
R14823 T19797 T19822 arg2Of and,electroporated
R14824 T19797 T19823 arg1Of and,using
R14825 T19800 T19797 arg2Of phRL-TK,and
R14826 T19800 T19798 arg1Of phRL-TK,0.5
R14827 T19800 T19799 arg1Of phRL-TK,μg
R14828 T19802 T19803 arg1Of added,to
R14829 T19802 T19821 arg1Of added,and
R14830 T19806 T19805 arg1Of 106,×
R14831 T19809 T19803 arg2Of cells,to
R14832 T19809 T19804 arg1Of cells,3
R14833 T19809 T19806 arg1Of cells,106
R14834 T19809 T19807 arg1Of cells,CD4+
R14835 T19809 T19808 arg1Of cells,T
R14836 T19809 T19810 arg2Of cells,resuspended
R14837 T19810 T19811 arg1Of resuspended,in
R14838 T19813 T19811 arg2Of μL,in
R14839 T19813 T19812 arg1Of μL,100
R14840 T19813 T19814 arg1Of μL,of
R14841 T19816 T19814 arg2Of solution,of
R14842 T19816 T19815 arg1Of solution,Nucleofector
R14843 T19816 T19817 arg1Of solution,(
R14844 T19819 T19817 arg2Of Biosystems,(
R14845 T19819 T19818 arg1Of Biosystems,Amaxa
R14846 T19820 T19817 arg3Of ),(
R14847 T19821 T19801 arg2Of and,were
R14848 T19822 T19821 arg2Of electroporated,and
R14849 T19823 T19822 arg3Of using,electroporated
R14850 T19823 T19830 arg1Of using,After
R14851 T19823 T19834 arg1Of using,in
R14852 T19826 T19823 arg2Of program,using
R14853 T19826 T19824 arg1Of program,the
R14854 T19826 T19825 arg1Of program,U-15
R14855 T19826 T19827 arg1Of program,of
R14856 T19829 T19827 arg2Of Nucleofector.,of
R14857 T19829 T19828 arg1Of Nucleofector.,the
R14858 T19833 T19830 arg2Of culture,After
R14859 T19833 T19831 arg1Of culture,a
R14860 T19833 T19832 arg1Of culture,24-h
R14861 T19836 T19837 arg1Of conditions,and
R14862 T19837 T19834 arg2Of and,in
R14863 T19837 T19835 arg1Of and,serum-free
R14864 T19837 T19839 arg1Of and,as
R14865 T19837 T19847 modOf and,was
R14866 T19838 T19837 arg2Of stimuli,and
R14867 T19840 T19839 arg2Of indicated,as
R14868 T19840 T19841 arg1Of indicated,in
R14869 T19843 T19841 arg2Of figures,in
R14870 T19843 T19842 arg1Of figures,the
R14871 T19846 T19845 arg1Of activity,luciferase
R14872 T19846 T19847 arg1Of activity,was
R14873 T19846 T19848 arg2Of activity,measured
R14874 T19848 T19844 arg1Of measured,","
R14875 T19848 T19847 arg2Of measured,was
R14876 T19848 T19849 arg1Of measured,by
R14877 T19848 T19859 arg1Of measured,according
R14878 T19854 T19849 arg2Of system,by
R14879 T19854 T19850 arg1Of system,the
R14880 T19854 T19851 arg1Of system,dual
R14881 T19854 T19852 arg1Of system,luciferase
R14882 T19854 T19853 arg1Of system,assay
R14883 T19854 T19855 arg1Of system,(
R14884 T19857 T19855 arg2Of Biotech,(
R14885 T19857 T19856 arg1Of Biotech,Promega
R14886 T19858 T19855 arg3Of ),(
R14887 T19860 T19859 arg2Of to,according
R14888 T19862 T19861 arg1Of manufacturer,the
R14889 T19862 T19863 arg2Of manufacturer,'s
R14890 T19864 T19860 arg2Of instructions,to
R14891 T19864 T19863 arg1Of instructions,'s

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T19905 0-3 CD denotes 3.5
T19906 4-6 NN denotes μg
T19907 7-9 IN denotes of
T19908 10-13 DT denotes the
T19909 14-19 CD denotes FOXP3
T19910 20-28 NN denotes promoter
T19911 29-39 NN denotes luciferase
T19912 40-48 NN denotes reporter
T19913 49-55 NN denotes vector
T19914 56-59 CC denotes and
T19915 60-63 CD denotes 0.5
T19916 64-66 NN denotes μg
T19917 67-74 NN denotes phRL-TK
T19918 75-79 VBD denotes were
T19919 80-85 VBN denotes added
T19920 86-88 TO denotes to
T19921 89-90 CD denotes 3
T19922 91-92 CD denotes ×
T19923 93-96 CD denotes 106
T19924 97-100 NNP denotes CD4
T19925 100-101 CD denotes +
T19926 102-103 NNP denotes T
T19927 104-109 NNS denotes cells
T19928 110-121 VBD denotes resuspended
T19929 122-124 IN denotes in
T19930 125-128 CD denotes 100
T19931 129-131 NN denotes μL
T19932 132-134 IN denotes of
T19933 135-147 NNP denotes Nucleofector
T19934 148-156 NN denotes solution
T19935 157-158 -LRB- denotes (
T19936 158-163 NNP denotes Amaxa
T19937 164-174 NNPS denotes Biosystems
T19938 174-175 -RRB- denotes )
T19939 176-179 CC denotes and
T19940 180-194 VBD denotes electroporated
T19941 195-200 VBG denotes using
T19942 201-204 DT denotes the
T19943 205-209 NN denotes U-15
T19944 210-217 NN denotes program
T19945 218-220 IN denotes of
T19946 221-224 DT denotes the
T19947 225-237 NNP denotes Nucleofector
T19948 237-238 . denotes .
T19949 239-244 IN denotes After
T19950 245-246 DT denotes a
T19951 247-251 JJ denotes 24-h
T19952 252-259 NN denotes culture
T19953 260-262 IN denotes in
T19954 263-273 JJ denotes serum-free
T19955 274-284 NNS denotes conditions
T19956 285-288 CC denotes and
T19957 289-296 NNS denotes stimuli
T19958 297-299 IN denotes as
T19959 300-309 VBN denotes indicated
T19960 310-312 IN denotes in
T19961 313-316 DT denotes the
T19962 317-324 NNS denotes figures
T19963 324-325 , denotes ,
T19964 326-336 NN denotes luciferase
T19965 337-345 NN denotes activity
T19966 346-349 VBD denotes was
T19967 350-358 VBN denotes measured
T19968 359-361 IN denotes by
T19969 362-365 DT denotes the
T19970 366-370 JJ denotes dual
T19971 371-381 NN denotes luciferase
T19972 382-387 NN denotes assay
T19973 388-394 NN denotes system
T19974 395-396 -LRB- denotes (
T19975 396-403 NNP denotes Promega
T19976 404-411 NNP denotes Biotech
T19977 411-412 -RRB- denotes )
T19978 413-422 VBG denotes according
T19979 423-425 TO denotes to
T19980 426-429 DT denotes the
T19981 430-442 NN denotes manufacturer
T19982 442-444 POS denotes 's
T19983 445-457 NNS denotes instructions
T19984 457-458 . denotes .
R14921 T19905 T19906 nummod 3.5,μg
R14922 T19906 T19919 nsubjpass μg,added
R14923 T19907 T19906 prep of,μg
R14924 T19908 T19913 det the,vector
R14925 T19909 T19913 nummod FOXP3,vector
R14926 T19910 T19913 nmod promoter,vector
R14927 T19911 T19913 nmod luciferase,vector
R14928 T19912 T19913 compound reporter,vector
R14929 T19913 T19907 pobj vector,of
R14930 T19914 T19906 cc and,μg
R14931 T19915 T19916 nummod 0.5,μg
R14932 T19916 T19917 compound μg,phRL-TK
R14933 T19917 T19906 conj phRL-TK,μg
R14934 T19918 T19919 auxpass were,added
R14935 T19919 T19919 ROOT added,added
R14936 T19920 T19919 prep to,added
R14937 T19921 T19923 quantmod 3,106
R14938 T19922 T19923 quantmod ×,106
R14939 T19923 T19927 nummod 106,cells
R14940 T19924 T19927 nmod CD4,cells
R14941 T19925 T19927 nummod +,cells
R14942 T19926 T19927 compound T,cells
R14943 T19927 T19920 pobj cells,to
R14944 T19928 T19919 oprd resuspended,added
R14945 T19929 T19928 prep in,resuspended
R14946 T19930 T19931 nummod 100,μL
R14947 T19931 T19929 pobj μL,in
R14948 T19932 T19931 prep of,μL
R14949 T19933 T19934 compound Nucleofector,solution
R14950 T19934 T19932 pobj solution,of
R14951 T19935 T19937 punct (,Biosystems
R14952 T19936 T19937 compound Amaxa,Biosystems
R14953 T19937 T19934 appos Biosystems,solution
R14954 T19938 T19937 punct ),Biosystems
R14955 T19939 T19928 cc and,resuspended
R14956 T19940 T19928 conj electroporated,resuspended
R14957 T19941 T19940 xcomp using,electroporated
R14958 T19942 T19944 det the,program
R14959 T19943 T19944 compound U-15,program
R14960 T19944 T19941 dobj program,using
R14961 T19945 T19944 prep of,program
R14962 T19946 T19947 det the,Nucleofector
R14963 T19947 T19945 pobj Nucleofector,of
R14964 T19948 T19919 punct .,added
R14965 T19949 T19967 prep After,measured
R14966 T19950 T19952 det a,culture
R14967 T19951 T19952 amod 24-h,culture
R14968 T19952 T19949 pobj culture,After
R14969 T19953 T19952 prep in,culture
R14970 T19954 T19955 amod serum-free,conditions
R14971 T19955 T19953 pobj conditions,in
R14972 T19956 T19955 cc and,conditions
R14973 T19957 T19955 conj stimuli,conditions
R14974 T19958 T19959 mark as,indicated
R14975 T19959 T19967 advcl indicated,measured
R14976 T19960 T19959 prep in,indicated
R14977 T19961 T19962 det the,figures
R14978 T19962 T19960 pobj figures,in
R14979 T19963 T19967 punct ",",measured
R14980 T19964 T19965 compound luciferase,activity
R14981 T19965 T19967 nsubjpass activity,measured
R14982 T19966 T19967 auxpass was,measured
R14983 T19967 T19967 ROOT measured,measured
R14984 T19968 T19967 agent by,measured
R14985 T19969 T19973 det the,system
R14986 T19970 T19973 amod dual,system
R14987 T19971 T19973 compound luciferase,system
R14988 T19972 T19973 compound assay,system
R14989 T19973 T19968 pobj system,by
R14990 T19974 T19973 punct (,system
R14991 T19975 T19976 compound Promega,Biotech
R14992 T19976 T19973 appos Biotech,system
R14993 T19977 T19973 punct ),system
R14994 T19978 T19967 prep according,measured
R14995 T19979 T19978 prep to,according
R14996 T19980 T19981 det the,manufacturer
R14997 T19981 T19983 poss manufacturer,instructions
R14998 T19982 T19981 case 's,manufacturer
R14999 T19983 T19979 pobj instructions,to
R15000 T19984 T19967 punct .,measured

UBERON-AE

Id Subject Object Predicate Lexical cue
T19754 263-268 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T19765 326-345 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T19766 326-345 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T19767 326-345 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T19768 326-345 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T19769 326-345 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity

GO-MF

Id Subject Object Predicate Lexical cue
T20001 326-345 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T20002 326-345 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T20003 326-345 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T20004 326-345 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T20005 326-345 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity

GO-CC

Id Subject Object Predicate Lexical cue
T20007 104-109 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T19763 0-458 Sentence denotes 3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions.
T293 0-238 Sentence denotes 3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector.
T294 239-458 Sentence denotes After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions.

events-check-again

Id Subject Object Predicate Lexical cue
T20030 14-19 Protein denotes FOXP3
T20031 29-39 Protein denotes luciferase
T20032 97-100 Protein denotes CD4
T20033 326-336 Protein denotes luciferase
T20034 371-381 Protein denotes luciferase

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T20008 14-48 Protein denotes FOXP3 promoter luciferase reporter
T20009 326-336 Protein denotes luciferase
T20010 371-381 Protein denotes luciferase

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T19755 14-19 Protein denotes FOXP3
T19756 29-39 Protein denotes luciferase
T19757 97-100 Protein denotes CD4
T19758 326-336 Protein denotes luciferase
T19759 371-381 Protein denotes luciferase

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T19875 14-19 Q9BZS1 denotes FOXP3
T19876 29-39 P08659 denotes luciferase
T19877 97-100 P01730 denotes CD4
T19878 326-336 P08659 denotes luciferase
T19879 371-381 P08659 denotes luciferase

test2

Id Subject Object Predicate Lexical cue
T19747 14-19 Protein denotes FOXP3
T19748 29-39 Protein denotes luciferase
T19749 97-100 Protein denotes CD4
T19750 326-336 Protein denotes luciferase
T19751 371-381 Protein denotes luciferase