PMC:2222968 / 39335-39971
Annnotations
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19770 | 0-13 | NN | denotes | Transfections |
T19771 | 14-17 | CC | denotes | and |
T19772 | 18-26 | NN | denotes | reporter |
T19773 | 27-31 | NN | denotes | gene |
T19774 | 32-38 | NN | denotes | assays |
T19775 | 40-41 | NN | denotes | T |
T19776 | 42-47 | NN | denotes | cells |
T19777 | 48-52 | VB | denotes | were |
T19778 | 53-59 | VB | denotes | rested |
T19779 | 60-62 | IN | denotes | in |
T19780 | 63-73 | JJ | denotes | serum-free |
T19781 | 74-79 | NN | denotes | AIM-V |
T19782 | 80-86 | NN | denotes | medium |
T19783 | 87-88 | -LRB- | denotes | ( |
T19784 | 88-92 | NNP | denotes | Life |
T19785 | 93-105 | NNP | denotes | Technologies |
T19786 | 105-106 | -RRB- | denotes | ) |
T19787 | 107-116 | RB | denotes | overnight |
T19788 | 118-121 | CD | denotes | 3.5 |
T19789 | 122-124 | NN | denotes | μg |
T19790 | 125-127 | IN | denotes | of |
T19791 | 128-131 | DT | denotes | the |
T19792 | 132-137 | NN | denotes | FOXP3 |
T19793 | 138-146 | NN | denotes | promoter |
T19794 | 147-157 | NN | denotes | luciferase |
T19795 | 158-166 | NN | denotes | reporter |
T19796 | 167-173 | NN | denotes | vector |
T19797 | 174-177 | CC | denotes | and |
T19798 | 178-181 | CD | denotes | 0.5 |
T19799 | 182-184 | NN | denotes | μg |
T19800 | 185-192 | NN | denotes | phRL-TK |
T19801 | 193-197 | VB | denotes | were |
T19802 | 198-203 | VB | denotes | added |
T19803 | 204-206 | TO | denotes | to |
T19804 | 207-208 | CD | denotes | 3 |
T19805 | 209-210 | SYM | denotes | × |
T19806 | 211-214 | CD | denotes | 106 |
T19807 | 215-219 | JJ | denotes | CD4+ |
T19808 | 220-221 | NN | denotes | T |
T19809 | 222-227 | NN | denotes | cells |
T19810 | 228-239 | VB | denotes | resuspended |
T19811 | 240-242 | IN | denotes | in |
T19812 | 243-246 | CD | denotes | 100 |
T19813 | 247-249 | NN | denotes | μL |
T19814 | 250-252 | IN | denotes | of |
T19815 | 253-265 | NN | denotes | Nucleofector |
T19816 | 266-274 | NN | denotes | solution |
T19817 | 275-276 | -LRB- | denotes | ( |
T19818 | 276-281 | NNP | denotes | Amaxa |
T19819 | 282-292 | NNP | denotes | Biosystems |
T19820 | 292-293 | -RRB- | denotes | ) |
T19821 | 294-297 | CC | denotes | and |
T19822 | 298-312 | VB | denotes | electroporated |
T19823 | 313-318 | VB | denotes | using |
T19824 | 319-322 | DT | denotes | the |
T19825 | 323-327 | NN | denotes | U-15 |
T19826 | 328-335 | NN | denotes | program |
T19827 | 336-338 | IN | denotes | of |
T19828 | 339-342 | DT | denotes | the |
T19829 | 343-356 | NNP | denotes | Nucleofector. |
T19830 | 357-362 | IN | denotes | After |
T19831 | 363-364 | DT | denotes | a |
T19832 | 365-369 | JJ | denotes | 24-h |
T19833 | 370-377 | NN | denotes | culture |
T19834 | 378-380 | IN | denotes | in |
T19835 | 381-391 | JJ | denotes | serum-free |
T19836 | 392-402 | NN | denotes | conditions |
T19837 | 403-406 | CC | denotes | and |
T19838 | 407-414 | NN | denotes | stimuli |
T19839 | 415-417 | IN | denotes | as |
T19840 | 418-427 | VB | denotes | indicated |
T19841 | 428-430 | IN | denotes | in |
T19842 | 431-434 | DT | denotes | the |
T19843 | 435-442 | NN | denotes | figures |
T19844 | 442-443 | -COMMA- | denotes | , |
T19845 | 444-454 | NN | denotes | luciferase |
T19846 | 455-463 | NN | denotes | activity |
T19847 | 464-467 | VB | denotes | was |
T19848 | 468-476 | VB | denotes | measured |
T19849 | 477-479 | IN | denotes | by |
T19850 | 480-483 | DT | denotes | the |
T19851 | 484-488 | JJ | denotes | dual |
T19852 | 489-499 | NN | denotes | luciferase |
T19853 | 500-505 | NN | denotes | assay |
T19854 | 506-512 | NN | denotes | system |
T19855 | 513-514 | -LRB- | denotes | ( |
T19856 | 514-521 | NNP | denotes | Promega |
T19857 | 522-529 | NNP | denotes | Biotech |
T19858 | 529-530 | -RRB- | denotes | ) |
T19859 | 531-540 | VB | denotes | according |
T19860 | 541-543 | TO | denotes | to |
T19861 | 544-547 | DT | denotes | the |
T19862 | 548-560 | NN | denotes | manufacturer |
T19863 | 560-562 | POS | denotes | 's |
T19864 | 563-575 | NN | denotes | instructions |
T19865 | 577-581 | NN | denotes | Data |
T19866 | 582-586 | VB | denotes | were |
T19867 | 587-597 | VB | denotes | normalized |
T19868 | 598-600 | IN | denotes | by |
T19869 | 601-604 | DT | denotes | the |
T19870 | 605-613 | NN | denotes | activity |
T19871 | 614-616 | IN | denotes | of |
T19872 | 617-624 | NN | denotes | renilla |
T19873 | 625-635 | NN | denotes | luciferase |
R14795 | T19770 | T19771 | arg1Of | Transfections,and |
R14796 | T19774 | T19771 | arg2Of | assays,and |
R14797 | T19774 | T19772 | arg1Of | assays,reporter |
R14798 | T19774 | T19773 | arg1Of | assays,gene |
R14799 | T19776 | T19775 | arg1Of | cells,T |
R14800 | T19776 | T19777 | arg1Of | cells,were |
R14801 | T19776 | T19778 | arg2Of | cells,rested |
R14802 | T19778 | T19777 | arg2Of | rested,were |
R14803 | T19778 | T19779 | arg1Of | rested,in |
R14804 | T19778 | T19787 | arg1Of | rested,overnight |
R14805 | T19782 | T19779 | arg2Of | medium,in |
R14806 | T19782 | T19780 | arg1Of | medium,serum-free |
R14807 | T19782 | T19781 | arg1Of | medium,AIM-V |
R14808 | T19782 | T19783 | arg1Of | medium,( |
R14809 | T19785 | T19783 | arg2Of | Technologies,( |
R14810 | T19785 | T19784 | arg1Of | Technologies,Life |
R14811 | T19786 | T19783 | arg3Of | ),( |
R14812 | T19789 | T19788 | arg1Of | μg,3.5 |
R14813 | T19789 | T19790 | arg1Of | μg,of |
R14814 | T19789 | T19797 | arg1Of | μg,and |
R14815 | T19796 | T19790 | arg2Of | vector,of |
R14816 | T19796 | T19791 | arg1Of | vector,the |
R14817 | T19796 | T19792 | arg1Of | vector,FOXP3 |
R14818 | T19796 | T19793 | arg1Of | vector,promoter |
R14819 | T19796 | T19794 | arg1Of | vector,luciferase |
R14820 | T19796 | T19795 | arg1Of | vector,reporter |
R14821 | T19797 | T19801 | arg1Of | and,were |
R14822 | T19797 | T19802 | arg2Of | and,added |
R14823 | T19797 | T19822 | arg2Of | and,electroporated |
R14824 | T19797 | T19823 | arg1Of | and,using |
R14825 | T19800 | T19797 | arg2Of | phRL-TK,and |
R14826 | T19800 | T19798 | arg1Of | phRL-TK,0.5 |
R14827 | T19800 | T19799 | arg1Of | phRL-TK,μg |
R14828 | T19802 | T19803 | arg1Of | added,to |
R14829 | T19802 | T19821 | arg1Of | added,and |
R14830 | T19806 | T19805 | arg1Of | 106,× |
R14831 | T19809 | T19803 | arg2Of | cells,to |
R14832 | T19809 | T19804 | arg1Of | cells,3 |
R14833 | T19809 | T19806 | arg1Of | cells,106 |
R14834 | T19809 | T19807 | arg1Of | cells,CD4+ |
R14835 | T19809 | T19808 | arg1Of | cells,T |
R14836 | T19809 | T19810 | arg2Of | cells,resuspended |
R14837 | T19810 | T19811 | arg1Of | resuspended,in |
R14838 | T19813 | T19811 | arg2Of | μL,in |
R14839 | T19813 | T19812 | arg1Of | μL,100 |
R14840 | T19813 | T19814 | arg1Of | μL,of |
R14841 | T19816 | T19814 | arg2Of | solution,of |
R14842 | T19816 | T19815 | arg1Of | solution,Nucleofector |
R14843 | T19816 | T19817 | arg1Of | solution,( |
R14844 | T19819 | T19817 | arg2Of | Biosystems,( |
R14845 | T19819 | T19818 | arg1Of | Biosystems,Amaxa |
R14846 | T19820 | T19817 | arg3Of | ),( |
R14847 | T19821 | T19801 | arg2Of | and,were |
R14848 | T19822 | T19821 | arg2Of | electroporated,and |
R14849 | T19823 | T19822 | arg3Of | using,electroporated |
R14850 | T19823 | T19830 | arg1Of | using,After |
R14851 | T19823 | T19834 | arg1Of | using,in |
R14852 | T19826 | T19823 | arg2Of | program,using |
R14853 | T19826 | T19824 | arg1Of | program,the |
R14854 | T19826 | T19825 | arg1Of | program,U-15 |
R14855 | T19826 | T19827 | arg1Of | program,of |
R14856 | T19829 | T19827 | arg2Of | Nucleofector.,of |
R14857 | T19829 | T19828 | arg1Of | Nucleofector.,the |
R14858 | T19833 | T19830 | arg2Of | culture,After |
R14859 | T19833 | T19831 | arg1Of | culture,a |
R14860 | T19833 | T19832 | arg1Of | culture,24-h |
R14861 | T19836 | T19837 | arg1Of | conditions,and |
R14862 | T19837 | T19834 | arg2Of | and,in |
R14863 | T19837 | T19835 | arg1Of | and,serum-free |
R14864 | T19837 | T19839 | arg1Of | and,as |
R14865 | T19837 | T19847 | modOf | and,was |
R14866 | T19838 | T19837 | arg2Of | stimuli,and |
R14867 | T19840 | T19839 | arg2Of | indicated,as |
R14868 | T19840 | T19841 | arg1Of | indicated,in |
R14869 | T19843 | T19841 | arg2Of | figures,in |
R14870 | T19843 | T19842 | arg1Of | figures,the |
R14871 | T19846 | T19845 | arg1Of | activity,luciferase |
R14872 | T19846 | T19847 | arg1Of | activity,was |
R14873 | T19846 | T19848 | arg2Of | activity,measured |
R14874 | T19848 | T19844 | arg1Of | measured,"," |
R14875 | T19848 | T19847 | arg2Of | measured,was |
R14876 | T19848 | T19849 | arg1Of | measured,by |
R14877 | T19848 | T19859 | arg1Of | measured,according |
R14878 | T19854 | T19849 | arg2Of | system,by |
R14879 | T19854 | T19850 | arg1Of | system,the |
R14880 | T19854 | T19851 | arg1Of | system,dual |
R14881 | T19854 | T19852 | arg1Of | system,luciferase |
R14882 | T19854 | T19853 | arg1Of | system,assay |
R14883 | T19854 | T19855 | arg1Of | system,( |
R14884 | T19857 | T19855 | arg2Of | Biotech,( |
R14885 | T19857 | T19856 | arg1Of | Biotech,Promega |
R14886 | T19858 | T19855 | arg3Of | ),( |
R14887 | T19860 | T19859 | arg2Of | to,according |
R14888 | T19862 | T19861 | arg1Of | manufacturer,the |
R14889 | T19862 | T19863 | arg2Of | manufacturer,'s |
R14890 | T19864 | T19860 | arg2Of | instructions,to |
R14891 | T19864 | T19863 | arg1Of | instructions,'s |
R14892 | T19865 | T19866 | arg1Of | Data,were |
R14893 | T19865 | T19867 | arg2Of | Data,normalized |
R14894 | T19867 | T19866 | arg2Of | normalized,were |
R14895 | T19870 | T19867 | arg1Of | activity,normalized |
R14896 | T19870 | T19868 | arg2Of | activity,by |
R14897 | T19870 | T19869 | arg1Of | activity,the |
R14898 | T19870 | T19871 | arg1Of | activity,of |
R14899 | T19873 | T19871 | arg2Of | luciferase,of |
R14900 | T19873 | T19872 | arg1Of | luciferase,renilla |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19885 | 0-13 | NNS | denotes | Transfections |
T19886 | 14-17 | CC | denotes | and |
T19887 | 18-26 | NN | denotes | reporter |
T19888 | 27-31 | NN | denotes | gene |
T19889 | 32-38 | NNS | denotes | assays |
T19890 | 38-39 | . | denotes | . |
T19891 | 40-41 | NNP | denotes | T |
T19892 | 42-47 | NNS | denotes | cells |
T19893 | 48-52 | VBD | denotes | were |
T19894 | 53-59 | VBN | denotes | rested |
T19895 | 60-62 | IN | denotes | in |
T19896 | 63-73 | JJ | denotes | serum-free |
T19897 | 74-79 | NNP | denotes | AIM-V |
T19898 | 80-86 | NN | denotes | medium |
T19899 | 87-88 | -LRB- | denotes | ( |
T19900 | 88-92 | NNP | denotes | Life |
T19901 | 93-105 | NNPS | denotes | Technologies |
T19902 | 105-106 | -RRB- | denotes | ) |
T19903 | 107-116 | RB | denotes | overnight |
T19904 | 116-117 | . | denotes | . |
T19905 | 118-121 | CD | denotes | 3.5 |
T19906 | 122-124 | NN | denotes | μg |
T19907 | 125-127 | IN | denotes | of |
T19908 | 128-131 | DT | denotes | the |
T19909 | 132-137 | CD | denotes | FOXP3 |
T19910 | 138-146 | NN | denotes | promoter |
T19911 | 147-157 | NN | denotes | luciferase |
T19912 | 158-166 | NN | denotes | reporter |
T19913 | 167-173 | NN | denotes | vector |
T19914 | 174-177 | CC | denotes | and |
T19915 | 178-181 | CD | denotes | 0.5 |
T19916 | 182-184 | NN | denotes | μg |
T19917 | 185-192 | NN | denotes | phRL-TK |
T19918 | 193-197 | VBD | denotes | were |
T19919 | 198-203 | VBN | denotes | added |
T19920 | 204-206 | TO | denotes | to |
T19921 | 207-208 | CD | denotes | 3 |
T19922 | 209-210 | CD | denotes | × |
T19923 | 211-214 | CD | denotes | 106 |
T19924 | 215-218 | NNP | denotes | CD4 |
T19925 | 218-219 | CD | denotes | + |
T19926 | 220-221 | NNP | denotes | T |
T19927 | 222-227 | NNS | denotes | cells |
T19928 | 228-239 | VBD | denotes | resuspended |
T19929 | 240-242 | IN | denotes | in |
T19930 | 243-246 | CD | denotes | 100 |
T19931 | 247-249 | NN | denotes | μL |
T19932 | 250-252 | IN | denotes | of |
T19933 | 253-265 | NNP | denotes | Nucleofector |
T19934 | 266-274 | NN | denotes | solution |
T19935 | 275-276 | -LRB- | denotes | ( |
T19936 | 276-281 | NNP | denotes | Amaxa |
T19937 | 282-292 | NNPS | denotes | Biosystems |
T19938 | 292-293 | -RRB- | denotes | ) |
T19939 | 294-297 | CC | denotes | and |
T19940 | 298-312 | VBD | denotes | electroporated |
T19941 | 313-318 | VBG | denotes | using |
T19942 | 319-322 | DT | denotes | the |
T19943 | 323-327 | NN | denotes | U-15 |
T19944 | 328-335 | NN | denotes | program |
T19945 | 336-338 | IN | denotes | of |
T19946 | 339-342 | DT | denotes | the |
T19947 | 343-355 | NNP | denotes | Nucleofector |
T19948 | 355-356 | . | denotes | . |
T19949 | 357-362 | IN | denotes | After |
T19950 | 363-364 | DT | denotes | a |
T19951 | 365-369 | JJ | denotes | 24-h |
T19952 | 370-377 | NN | denotes | culture |
T19953 | 378-380 | IN | denotes | in |
T19954 | 381-391 | JJ | denotes | serum-free |
T19955 | 392-402 | NNS | denotes | conditions |
T19956 | 403-406 | CC | denotes | and |
T19957 | 407-414 | NNS | denotes | stimuli |
T19958 | 415-417 | IN | denotes | as |
T19959 | 418-427 | VBN | denotes | indicated |
T19960 | 428-430 | IN | denotes | in |
T19961 | 431-434 | DT | denotes | the |
T19962 | 435-442 | NNS | denotes | figures |
T19963 | 442-443 | , | denotes | , |
T19964 | 444-454 | NN | denotes | luciferase |
T19965 | 455-463 | NN | denotes | activity |
T19966 | 464-467 | VBD | denotes | was |
T19967 | 468-476 | VBN | denotes | measured |
T19968 | 477-479 | IN | denotes | by |
T19969 | 480-483 | DT | denotes | the |
T19970 | 484-488 | JJ | denotes | dual |
T19971 | 489-499 | NN | denotes | luciferase |
T19972 | 500-505 | NN | denotes | assay |
T19973 | 506-512 | NN | denotes | system |
T19974 | 513-514 | -LRB- | denotes | ( |
T19975 | 514-521 | NNP | denotes | Promega |
T19976 | 522-529 | NNP | denotes | Biotech |
T19977 | 529-530 | -RRB- | denotes | ) |
T19978 | 531-540 | VBG | denotes | according |
T19979 | 541-543 | TO | denotes | to |
T19980 | 544-547 | DT | denotes | the |
T19981 | 548-560 | NN | denotes | manufacturer |
T19982 | 560-562 | POS | denotes | 's |
T19983 | 563-575 | NNS | denotes | instructions |
T19984 | 575-576 | . | denotes | . |
T19985 | 577-581 | NNS | denotes | Data |
T19986 | 582-586 | VBD | denotes | were |
T19987 | 587-597 | VBN | denotes | normalized |
T19988 | 598-600 | IN | denotes | by |
T19989 | 601-604 | DT | denotes | the |
T19990 | 605-613 | NN | denotes | activity |
T19991 | 614-616 | IN | denotes | of |
T19992 | 617-624 | NN | denotes | renilla |
T19993 | 625-635 | NN | denotes | luciferase |
T19994 | 635-636 | . | denotes | . |
R14901 | T19885 | T19885 | ROOT | Transfections,Transfections |
R14902 | T19886 | T19885 | cc | and,Transfections |
R14903 | T19887 | T19889 | compound | reporter,assays |
R14904 | T19888 | T19889 | compound | gene,assays |
R14905 | T19889 | T19885 | conj | assays,Transfections |
R14906 | T19890 | T19885 | punct | .,Transfections |
R14907 | T19891 | T19892 | compound | T,cells |
R14908 | T19892 | T19894 | nsubjpass | cells,rested |
R14909 | T19893 | T19894 | auxpass | were,rested |
R14910 | T19894 | T19894 | ROOT | rested,rested |
R14911 | T19895 | T19894 | prep | in,rested |
R14912 | T19896 | T19898 | amod | serum-free,medium |
R14913 | T19897 | T19898 | compound | AIM-V,medium |
R14914 | T19898 | T19895 | pobj | medium,in |
R14915 | T19899 | T19901 | punct | (,Technologies |
R14916 | T19900 | T19901 | compound | Life,Technologies |
R14917 | T19901 | T19898 | appos | Technologies,medium |
R14918 | T19902 | T19898 | punct | ),medium |
R14919 | T19903 | T19894 | advmod | overnight,rested |
R14920 | T19904 | T19894 | punct | .,rested |
R14921 | T19905 | T19906 | nummod | 3.5,μg |
R14922 | T19906 | T19919 | nsubjpass | μg,added |
R14923 | T19907 | T19906 | prep | of,μg |
R14924 | T19908 | T19913 | det | the,vector |
R14925 | T19909 | T19913 | nummod | FOXP3,vector |
R14926 | T19910 | T19913 | nmod | promoter,vector |
R14927 | T19911 | T19913 | nmod | luciferase,vector |
R14928 | T19912 | T19913 | compound | reporter,vector |
R14929 | T19913 | T19907 | pobj | vector,of |
R14930 | T19914 | T19906 | cc | and,μg |
R14931 | T19915 | T19916 | nummod | 0.5,μg |
R14932 | T19916 | T19917 | compound | μg,phRL-TK |
R14933 | T19917 | T19906 | conj | phRL-TK,μg |
R14934 | T19918 | T19919 | auxpass | were,added |
R14935 | T19919 | T19919 | ROOT | added,added |
R14936 | T19920 | T19919 | prep | to,added |
R14937 | T19921 | T19923 | quantmod | 3,106 |
R14938 | T19922 | T19923 | quantmod | ×,106 |
R14939 | T19923 | T19927 | nummod | 106,cells |
R14940 | T19924 | T19927 | nmod | CD4,cells |
R14941 | T19925 | T19927 | nummod | +,cells |
R14942 | T19926 | T19927 | compound | T,cells |
R14943 | T19927 | T19920 | pobj | cells,to |
R14944 | T19928 | T19919 | oprd | resuspended,added |
R14945 | T19929 | T19928 | prep | in,resuspended |
R14946 | T19930 | T19931 | nummod | 100,μL |
R14947 | T19931 | T19929 | pobj | μL,in |
R14948 | T19932 | T19931 | prep | of,μL |
R14949 | T19933 | T19934 | compound | Nucleofector,solution |
R14950 | T19934 | T19932 | pobj | solution,of |
R14951 | T19935 | T19937 | punct | (,Biosystems |
R14952 | T19936 | T19937 | compound | Amaxa,Biosystems |
R14953 | T19937 | T19934 | appos | Biosystems,solution |
R14954 | T19938 | T19937 | punct | ),Biosystems |
R14955 | T19939 | T19928 | cc | and,resuspended |
R14956 | T19940 | T19928 | conj | electroporated,resuspended |
R14957 | T19941 | T19940 | xcomp | using,electroporated |
R14958 | T19942 | T19944 | det | the,program |
R14959 | T19943 | T19944 | compound | U-15,program |
R14960 | T19944 | T19941 | dobj | program,using |
R14961 | T19945 | T19944 | prep | of,program |
R14962 | T19946 | T19947 | det | the,Nucleofector |
R14963 | T19947 | T19945 | pobj | Nucleofector,of |
R14964 | T19948 | T19919 | punct | .,added |
R14965 | T19949 | T19967 | prep | After,measured |
R14966 | T19950 | T19952 | det | a,culture |
R14967 | T19951 | T19952 | amod | 24-h,culture |
R14968 | T19952 | T19949 | pobj | culture,After |
R14969 | T19953 | T19952 | prep | in,culture |
R14970 | T19954 | T19955 | amod | serum-free,conditions |
R14971 | T19955 | T19953 | pobj | conditions,in |
R14972 | T19956 | T19955 | cc | and,conditions |
R14973 | T19957 | T19955 | conj | stimuli,conditions |
R14974 | T19958 | T19959 | mark | as,indicated |
R14975 | T19959 | T19967 | advcl | indicated,measured |
R14976 | T19960 | T19959 | prep | in,indicated |
R14977 | T19961 | T19962 | det | the,figures |
R14978 | T19962 | T19960 | pobj | figures,in |
R14979 | T19963 | T19967 | punct | ",",measured |
R14980 | T19964 | T19965 | compound | luciferase,activity |
R14981 | T19965 | T19967 | nsubjpass | activity,measured |
R14982 | T19966 | T19967 | auxpass | was,measured |
R14983 | T19967 | T19967 | ROOT | measured,measured |
R14984 | T19968 | T19967 | agent | by,measured |
R14985 | T19969 | T19973 | det | the,system |
R14986 | T19970 | T19973 | amod | dual,system |
R14987 | T19971 | T19973 | compound | luciferase,system |
R14988 | T19972 | T19973 | compound | assay,system |
R14989 | T19973 | T19968 | pobj | system,by |
R14990 | T19974 | T19973 | punct | (,system |
R14991 | T19975 | T19976 | compound | Promega,Biotech |
R14992 | T19976 | T19973 | appos | Biotech,system |
R14993 | T19977 | T19973 | punct | ),system |
R14994 | T19978 | T19967 | prep | according,measured |
R14995 | T19979 | T19978 | prep | to,according |
R14996 | T19980 | T19981 | det | the,manufacturer |
R14997 | T19981 | T19983 | poss | manufacturer,instructions |
R14998 | T19982 | T19981 | case | 's,manufacturer |
R14999 | T19983 | T19979 | pobj | instructions,to |
R15000 | T19984 | T19967 | punct | .,measured |
R15001 | T19985 | T19987 | nsubjpass | Data,normalized |
R15002 | T19986 | T19987 | auxpass | were,normalized |
R15003 | T19987 | T19987 | ROOT | normalized,normalized |
R15004 | T19988 | T19987 | agent | by,normalized |
R15005 | T19989 | T19990 | det | the,activity |
R15006 | T19990 | T19988 | pobj | activity,by |
R15007 | T19991 | T19990 | prep | of,activity |
R15008 | T19992 | T19993 | compound | renilla,luciferase |
R15009 | T19993 | T19991 | pobj | luciferase,of |
R15010 | T19994 | T19987 | punct | .,normalized |
UBERON-AE
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19753 | 63-68 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
T19754 | 381-386 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19765 | 444-463 | http://purl.obolibrary.org/obo/GO_0045289 | denotes | luciferase activity |
T19766 | 444-463 | http://purl.obolibrary.org/obo/GO_0047077 | denotes | luciferase activity |
T19767 | 444-463 | http://purl.obolibrary.org/obo/GO_0047712 | denotes | luciferase activity |
T19768 | 444-463 | http://purl.obolibrary.org/obo/GO_0050248 | denotes | luciferase activity |
T19769 | 444-463 | http://purl.obolibrary.org/obo/GO_0050397 | denotes | luciferase activity |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20001 | 444-463 | http://purl.obolibrary.org/obo/GO_0045289 | denotes | luciferase activity |
T20002 | 444-463 | http://purl.obolibrary.org/obo/GO_0047077 | denotes | luciferase activity |
T20003 | 444-463 | http://purl.obolibrary.org/obo/GO_0047712 | denotes | luciferase activity |
T20004 | 444-463 | http://purl.obolibrary.org/obo/GO_0050248 | denotes | luciferase activity |
T20005 | 444-463 | http://purl.obolibrary.org/obo/GO_0050397 | denotes | luciferase activity |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20006 | 42-47 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T20007 | 222-227 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19761 | 0-39 | Sentence | denotes | Transfections and reporter gene assays. |
T19762 | 40-117 | Sentence | denotes | T cells were rested in serum-free AIM-V medium (Life Technologies) overnight. |
T19763 | 118-576 | Sentence | denotes | 3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions. |
T19764 | 577-636 | Sentence | denotes | Data were normalized by the activity of renilla luciferase. |
T291 | 0-39 | Sentence | denotes | Transfections and reporter gene assays. |
T292 | 40-117 | Sentence | denotes | T cells were rested in serum-free AIM-V medium (Life Technologies) overnight. |
T293 | 118-356 | Sentence | denotes | 3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. |
T294 | 357-576 | Sentence | denotes | After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions. |
T295 | 577-636 | Sentence | denotes | Data were normalized by the activity of renilla luciferase. |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20030 | 132-137 | Protein | denotes | FOXP3 |
T20031 | 147-157 | Protein | denotes | luciferase |
T20032 | 215-218 | Protein | denotes | CD4 |
T20033 | 444-454 | Protein | denotes | luciferase |
T20034 | 489-499 | Protein | denotes | luciferase |
T20035 | 625-635 | Protein | denotes | luciferase |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20008 | 132-166 | Protein | denotes | FOXP3 promoter luciferase reporter |
T20009 | 444-454 | Protein | denotes | luciferase |
T20010 | 489-499 | Protein | denotes | luciferase |
T20011 | 617-635 | Protein | denotes | renilla luciferase |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19755 | 132-137 | Protein | denotes | FOXP3 |
T19756 | 147-157 | Protein | denotes | luciferase |
T19757 | 215-218 | Protein | denotes | CD4 |
T19758 | 444-454 | Protein | denotes | luciferase |
T19759 | 489-499 | Protein | denotes | luciferase |
T19760 | 625-635 | Protein | denotes | luciferase |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19874 | 74-77 | P26358 | denotes | AIM |
T19875 | 132-137 | Q9BZS1 | denotes | FOXP3 |
T19876 | 147-157 | P08659 | denotes | luciferase |
T19877 | 215-218 | P01730 | denotes | CD4 |
T19878 | 444-454 | P08659 | denotes | luciferase |
T19879 | 489-499 | P08659 | denotes | luciferase |
T19880 | 625-635 | P08659 | denotes | luciferase |
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19747 | 132-137 | Protein | denotes | FOXP3 |
T19748 | 147-157 | Protein | denotes | luciferase |
T19749 | 215-218 | Protein | denotes | CD4 |
T19750 | 444-454 | Protein | denotes | luciferase |
T19751 | 489-499 | Protein | denotes | luciferase |
T19752 | 625-635 | Protein | denotes | luciferase |