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{"project":"GO-MF","denotations":[{"id":"T19010","span":{"begin":498,"end":500},"obj":"http://purl.obolibrary.org/obo/GO_0003964"}],"text":"Inducible murine Treg culture.\nNaive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from pooled lymph nodes and spleens by FACS (FACS Aria, BD Biosciences). 5 × 105 T cells were co-cultured with 2.5 × 104 bone marrow–derived dendritic cells [62] and 0.01 μg/ml OVA323–339 peptide (Ansynth) in the presence or absence of 20-ng/μl rhTGF-ß1 (Peprotech) in 48-well plates. After 4 d, cells were harvested and analyzed for intracellular FOXP3 expression by FACS or gene expression by quantitative RT-PCR."}

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{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T19016","span":{"begin":37,"end":41},"obj":"Protein"},{"id":"T19017","span":{"begin":51,"end":54},"obj":"Protein"},{"id":"T19018","span":{"begin":57,"end":62},"obj":"Protein"},{"id":"T19019","span":{"begin":69,"end":74},"obj":"Protein"},{"id":"T19020","span":{"begin":335,"end":343},"obj":"Protein"},{"id":"T19021","span":{"begin":438,"end":443},"obj":"Protein"},{"id":"T19022","span":{"begin":444,"end":454},"obj":"Gene_expression"}],"relations":[{"id":"R14362","pred":"themeOf","subj":"T19021","obj":"T19022"}],"text":"Inducible murine Treg culture.\nNaive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from pooled lymph nodes and spleens by FACS (FACS Aria, BD Biosciences). 5 × 105 T cells were co-cultured with 2.5 × 104 bone marrow–derived dendritic cells [62] and 0.01 μg/ml OVA323–339 peptide (Ansynth) in the presence or absence of 20-ng/μl rhTGF-ß1 (Peprotech) in 48-well plates. After 4 d, cells were harvested and analyzed for intracellular FOXP3 expression by FACS or gene expression by quantitative RT-PCR."}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T18780","span":{"begin":37,"end":40},"obj":"Protein"},{"id":"T18781","span":{"begin":51,"end":54},"obj":"Protein"},{"id":"T18782","span":{"begin":57,"end":62},"obj":"Protein"},{"id":"T18783","span":{"begin":69,"end":73},"obj":"Protein"},{"id":"T18784","span":{"begin":337,"end":343},"obj":"Protein"},{"id":"T18785","span":{"begin":438,"end":443},"obj":"Protein"},{"id":"T18786","span":{"begin":444,"end":454},"obj":"Gene_expression"}],"relations":[{"id":"R14158","pred":"themeOf","subj":"T18785","obj":"T18786"}],"attributes":[{"id":"M437","pred":"Speculation","subj":"T18786","obj":"true"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Inducible murine Treg culture.\nNaive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from pooled lymph nodes and spleens by FACS (FACS Aria, BD Biosciences). 5 × 105 T cells were co-cultured with 2.5 × 104 bone marrow–derived dendritic cells [62] and 0.01 μg/ml OVA323–339 peptide (Ansynth) in the presence or absence of 20-ng/μl rhTGF-ß1 (Peprotech) in 48-well plates. After 4 d, cells were harvested and analyzed for intracellular FOXP3 expression by FACS or gene expression by quantitative RT-PCR."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T18887","span":{"begin":37,"end":40},"obj":"P01730"},{"id":"T18888","span":{"begin":51,"end":54},"obj":"P01730"},{"id":"T18889","span":{"begin":57,"end":62},"obj":"P14151"},{"id":"T18890","span":{"begin":69,"end":73},"obj":"P01589"},{"id":"T18891","span":{"begin":438,"end":443},"obj":"Q9BZS1"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Inducible murine Treg culture.\nNaive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from pooled lymph nodes and spleens by FACS (FACS Aria, BD Biosciences). 5 × 105 T cells were co-cultured with 2.5 × 104 bone marrow–derived dendritic cells [62] and 0.01 μg/ml OVA323–339 peptide (Ansynth) in the presence or absence of 20-ng/μl rhTGF-ß1 (Peprotech) in 48-well plates. After 4 d, cells were harvested and analyzed for intracellular FOXP3 expression by FACS or gene expression by quantitative RT-PCR."}

{"project":"test2","denotations":[{"id":"T18769","span":{"begin":37,"end":40},"obj":"Protein"},{"id":"T18770","span":{"begin":51,"end":54},"obj":"Protein"},{"id":"T18771","span":{"begin":57,"end":62},"obj":"Protein"},{"id":"T18772","span":{"begin":69,"end":73},"obj":"Protein"},{"id":"T18773","span":{"begin":340,"end":343},"obj":"Protein"},{"id":"T18774","span":{"begin":438,"end":443},"obj":"Protein"},{"id":"T18775","span":{"begin":444,"end":454},"obj":"Gene_expression"}],"relations":[{"id":"R14157","pred":"themeOf","subj":"T18774","obj":"T18775"}],"attributes":[{"id":"M436","pred":"Speculation","subj":"T18769","obj":"true"}],"text":"Inducible murine Treg culture.\nNaive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from pooled lymph nodes and spleens by FACS (FACS Aria, BD Biosciences). 5 × 105 T cells were co-cultured with 2.5 × 104 bone marrow–derived dendritic cells [62] and 0.01 μg/ml OVA323–339 peptide (Ansynth) in the presence or absence of 20-ng/μl rhTGF-ß1 (Peprotech) in 48-well plates. After 4 d, cells were harvested and analyzed for intracellular FOXP3 expression by FACS or gene expression by quantitative RT-PCR."}