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ubj":"T18248","obj":"T18243"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    UBERON-AE

    {"project":"UBERON-AE","denotations":[{"id":"T18065","span":{"begin":59,"end":64},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    GO-BP

    {"project":"GO-BP","denotations":[{"id":"T18078","span":{"begin":302,"end":305},"obj":"http://purl.obolibrary.org/obo/GO_0006283"},{"id":"T18079","span":{"begin":400,"end":414},"obj":"http://purl.obolibrary.org/obo/GO_0072602"},{"id":"T18080","span":{"begin":405,"end":414},"obj":"http://purl.obolibrary.org/obo/GO_0046903"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    GO-MF

    {"project":"GO-MF","denotations":[{"id":"T18256","span":{"begin":400,"end":404},"obj":"http://purl.obolibrary.org/obo/GO_0005136"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    GO-CC

    {"project":"GO-CC","denotations":[{"id":"T18257","span":{"begin":20,"end":25},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T18258","span":{"begin":34,"end":39},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T18259","span":{"begin":204,"end":209},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T18260","span":{"begin":273,"end":277},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T18261","span":{"begin":302,"end":305},"obj":"http://purl.obolibrary.org/obo/GO_0042101"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    sentences

    {"project":"sentences","denotations":[{"id":"T18073","span":{"begin":0,"end":26},"obj":"Sentence"},{"id":"T18074","span":{"begin":27,"end":182},"obj":"Sentence"},{"id":"T18075","span":{"begin":183,"end":253},"obj":"Sentence"},{"id":"T18076","span":{"begin":254,"end":361},"obj":"Sentence"},{"id":"T18077","span":{"begin":362,"end":415},"obj":"Sentence"},{"id":"T263","span":{"begin":0,"end":26},"obj":"Sentence"},{"id":"T264","span":{"begin":27,"end":182},"obj":"Sentence"},{"id":"T265","span":{"begin":183,"end":253},"obj":"Sentence"},{"id":"T266","span":{"begin":254,"end":361},"obj":"Sentence"},{"id":"T267","span":{"begin":362,"end":415},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    events-check-again

    {"project":"events-check-again","denotations":[{"id":"T18290","span":{"begin":13,"end":16},"obj":"Protein"},{"id":"T18291","span":{"begin":27,"end":30},"obj":"Protein"},{"id":"T18292","span":{"begin":108,"end":111},"obj":"Protein"},{"id":"T18293","span":{"begin":197,"end":200},"obj":"Protein"},{"id":"T18294","span":{"begin":395,"end":399},"obj":"Positive_regulation"},{"id":"T18295","span":{"begin":400,"end":404},"obj":"Protein"},{"id":"T18296","span":{"begin":405,"end":414},"obj":"Gene_expression"}],"relations":[{"id":"R13821","pred":"themeOf","subj":"T18295","obj":"T18296"},{"id":"R13822","pred":"themeOf","subj":"T18296","obj":"T18294"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    bionlp-st-ge-2016-reference-tees

    {"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T18262","span":{"begin":13,"end":17},"obj":"Protein"},{"id":"T18263","span":{"begin":27,"end":31},"obj":"Protein"},{"id":"T18264","span":{"begin":197,"end":201},"obj":"Protein"},{"id":"T18265","span":{"begin":345,"end":351},"obj":"Protein"},{"id":"T18266","span":{"begin":352,"end":360},"obj":"Gene_expression"},{"id":"T18267","span":{"begin":400,"end":404},"obj":"Protein"},{"id":"T18268","span":{"begin":405,"end":414},"obj":"Localization"}],"relations":[{"id":"R13813","pred":"themeOf","subj":"T18265","obj":"T18266"},{"id":"R13814","pred":"themeOf","subj":"T18267","obj":"T18268"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    bionlp-st-ge-2016-reference

    {"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T18066","span":{"begin":13,"end":16},"obj":"Protein"},{"id":"T18067","span":{"begin":27,"end":30},"obj":"Protein"},{"id":"T18068","span":{"begin":108,"end":111},"obj":"Protein"},{"id":"T18069","span":{"begin":197,"end":200},"obj":"Protein"},{"id":"T18070","span":{"begin":395,"end":399},"obj":"Positive_regulation"},{"id":"T18071","span":{"begin":400,"end":404},"obj":"Protein"},{"id":"T18072","span":{"begin":405,"end":414},"obj":"Gene_expression"}],"relations":[{"id":"R13647","pred":"themeOf","subj":"T18071","obj":"T18072"},{"id":"R13648","pred":"themeOf","subj":"T18072","obj":"T18070"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    bionlp-st-ge-2016-uniprot

    {"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T18156","span":{"begin":13,"end":16},"obj":"P01730"},{"id":"T18157","span":{"begin":27,"end":30},"obj":"P01730"},{"id":"T18158","span":{"begin":108,"end":111},"obj":"P01730"},{"id":"T18159","span":{"begin":197,"end":200},"obj":"P01730"},{"id":"T18160","span":{"begin":400,"end":404},"obj":"P05112"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}

    test2

    {"project":"test2","denotations":[{"id":"T18058","span":{"begin":13,"end":16},"obj":"Protein"},{"id":"T18059","span":{"begin":27,"end":30},"obj":"Protein"},{"id":"T18060","span":{"begin":108,"end":111},"obj":"Protein"},{"id":"T18061","span":{"begin":197,"end":200},"obj":"Protein"},{"id":"T18062","span":{"begin":395,"end":399},"obj":"Positive_regulation"},{"id":"T18063","span":{"begin":400,"end":404},"obj":"Protein"},{"id":"T18064","span":{"begin":405,"end":414},"obj":"Localization"}],"relations":[{"id":"R13645","pred":"themeOf","subj":"T18063","obj":"T18064"},{"id":"R13646","pred":"themeOf","subj":"T18064","obj":"T18062"}],"text":"Isolation of CD4+ T cells.\nCD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60]. The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%. Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive. The clones were characterized by high IL-4 secretion."}