PMC:2063610 / 40389-41322 JSONTXT

{"project":"2_test","denotations":[{"id":"17381551-12361602-1491361","span":{"begin":136,"end":140},"obj":"12361602"},{"id":"17381551-14645852-1491362","span":{"begin":157,"end":161},"obj":"14645852"},{"id":"17381551-12757751-1491363","span":{"begin":927,"end":931},"obj":"12757751"}],"text":"Myofiber explant cultures\nExplant and primary cell cultures were generated from C57-BL/6 mice, as previously described (Conboy \u0026 Rando, 2002; Conboy et al., 2003). Dissected gastrocnemius and tibialis anterior muscles underwent enzymatic digestion at 37 °C in DMEM (Invitrogen)/Pen-Strep (Invitrogen)/0.2% Collagenase Type IIA (Sigma) solution. Isolated fibers were resuspended in GM (Ham's F10 nutrient mixture (Mediatech, Inc., Herndon, VA, USA), 20% FBS (Mediatech), 5 ng mL−1 bFGF (Chemicon, Temecula, CA, USA) and 1% Pen-Strep, and cultured on ECM-coated (BD Biosciences, San Jose, CA, USA) plates (diluted 1 : 500 in PBS). Cultures of primary myoblasts were derived from isolated fibers, through repeated passaging, and were maintained in GM. Myoblast differentiation medium [DMEM, supplemented with 2% horse serum (Mediatech)] was used to promote rapid formation of myotubes from cultured myoblasts (Morgan \u0026 Partridge, 2003)."}

{"project":"CellFinder","denotations":[{"id":"T540","span":{"begin":480,"end":484},"obj":"GeneProtein"},{"id":"T541","span":{"begin":192,"end":216},"obj":"Anatomy"},{"id":"T542","span":{"begin":896,"end":905},"obj":"CellType"},{"id":"T543","span":{"begin":873,"end":881},"obj":"CellType"},{"id":"T544","span":{"begin":641,"end":658},"obj":"CellType"},{"id":"T545","span":{"begin":749,"end":757},"obj":"CellType"},{"id":"T546","span":{"begin":809,"end":814},"obj":"Species"},{"id":"T547","span":{"begin":38,"end":50},"obj":"CellType"},{"id":"T548","span":{"begin":210,"end":217},"obj":"Anatomy"},{"id":"T549","span":{"begin":174,"end":187},"obj":"Anatomy"},{"id":"T550","span":{"begin":89,"end":93},"obj":"Species"},{"id":"T551","span":{"begin":649,"end":658},"obj":"CellType"},{"id":"T552","span":{"begin":192,"end":209},"obj":"Anatomy"},{"id":"T553","span":{"begin":306,"end":326},"obj":"GeneProtein"},{"id":"T554","span":{"begin":0,"end":8},"obj":"Anatomy"}],"text":"Myofiber explant cultures\nExplant and primary cell cultures were generated from C57-BL/6 mice, as previously described (Conboy \u0026 Rando, 2002; Conboy et al., 2003). Dissected gastrocnemius and tibialis anterior muscles underwent enzymatic digestion at 37 °C in DMEM (Invitrogen)/Pen-Strep (Invitrogen)/0.2% Collagenase Type IIA (Sigma) solution. Isolated fibers were resuspended in GM (Ham's F10 nutrient mixture (Mediatech, Inc., Herndon, VA, USA), 20% FBS (Mediatech), 5 ng mL−1 bFGF (Chemicon, Temecula, CA, USA) and 1% Pen-Strep, and cultured on ECM-coated (BD Biosciences, San Jose, CA, USA) plates (diluted 1 : 500 in PBS). Cultures of primary myoblasts were derived from isolated fibers, through repeated passaging, and were maintained in GM. Myoblast differentiation medium [DMEM, supplemented with 2% horse serum (Mediatech)] was used to promote rapid formation of myotubes from cultured myoblasts (Morgan \u0026 Partridge, 2003)."}