PMC:1941754 / 45267-45745 JSONTXT

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    craft-sa-dev

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I spermatocyte spreads and OA treatment.\nMetaphase fixed spermatocytes from 8-mo-old Trip13RRB047 homozygotes, using 23-d-old wild-type mice as control, were prepared and stained with Giemsa as described [64].\nFor OA treatment, cells were exposed to 5 μM OA (Calbiochem, http://www.emdbiosciences.com) for 6 h at 32 °C in a humidified environment of 5% CO2 before spreading [65]. These preparations were stained with DAPI to visualize metaphase nuclei and chromosomes."}

    craft-ca-core-dev

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    2_test

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    craft-ca-core-ex-dev

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