Metaphase I spermatocyte spreads and OA treatment.
Metaphase fixed spermatocytes from 8-mo-old Trip13RRB047 homozygotes, using 23-d-old wild-type mice as control, were prepared and stained with Giemsa as described [64].
For OA treatment, cells were exposed to 5 μM OA (Calbiochem, http://www.emdbiosciences.com) for 6 h at 32 °C in a humidified environment of 5% CO2 before spreading [65]. These preparations were stained with DAPI to visualize metaphase nuclei and chromosomes.