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RNA was isolated from adult testes with the RNeasy Mini Kit (Qiagen, http://www.qiagen.com), and 4.0 μg was oligo dT–primed and reverse-transcribed with Superscript II (Stratagene, http://www.stratagene.com). The entire Trip13 protein-coding sequence was amplified with primers 5′-ATGGACGAGGCGGTG-3′ and 5′-TCAAACATAAGCTGAAAGTT-3′. The cycling conditions were: 94 °C 2 min; 94 °C 30s, 55 °C 45 s, and 72 °C 80 s for 35 cycles; and 72 °C 2 min. The primers for amplifying the Med31 coding sequence as control were : 5′-ATGGCCGCGGCCGTCGCTATGG-3′ and 5′-TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC-3′. The cycling conditions were: 94 °C 2 min; 94 °C 30 s, 55 °C 30 s, and 72 °C 35 s for 35 cycles; and 72 °C 2 min."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T8694","span":{"begin":28,"end":33},"obj":"UBERON:0007023"},{"id":"T8695","span":{"begin":34,"end":40},"obj":"UBERON:0000473"},{"id":"T8696","span":{"begin":134,"end":153},"obj":"GO:0001171"},{"id":"T8697","span":{"begin":226,"end":232},"obj":"PR:000016672"},{"id":"T8698","span":{"begin":233,"end":247},"obj":"SO:0000010"},{"id":"T8699","span":{"begin":276,"end":283},"obj":"SO:0000112"},{"id":"T8700","span":{"begin":454,"end":461},"obj":"SO:0000112"},{"id":"T8701","span":{"begin":481,"end":486},"obj":"PR:000010303"}],"text":"Total RNA was isolated from adult testes with the RNeasy Mini Kit (Qiagen, http://www.qiagen.com), and 4.0 μg was oligo dT–primed and reverse-transcribed with Superscript II (Stratagene, http://www.stratagene.com). The entire Trip13 protein-coding sequence was amplified with primers 5′-ATGGACGAGGCGGTG-3′ and 5′-TCAAACATAAGCTGAAAGTT-3′. The cycling conditions were: 94 °C 2 min; 94 °C 30s, 55 °C 45 s, and 72 °C 80 s for 35 cycles; and 72 °C 2 min. The primers for amplifying the Med31 coding sequence as control were : 5′-ATGGCCGCGGCCGTCGCTATGG-3′ and 5′-TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC-3′. The cycling conditions were: 94 °C 2 min; 94 °C 30 s, 55 °C 30 s, and 72 °C 35 s for 35 cycles; and 72 °C 2 min."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T8703","span":{"begin":6,"end":9},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T8704","span":{"begin":28,"end":33},"obj":"UBERON:0007023"},{"id":"T8705","span":{"begin":34,"end":40},"obj":"UBERON:0000473"},{"id":"T8706","span":{"begin":114,"end":119},"obj":"CHEBI_SO_EXT:oligonucleotide"},{"id":"T8707","span":{"begin":120,"end":122},"obj":"CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group"},{"id":"T8708","span":{"begin":134,"end":153},"obj":"GO:0001171"},{"id":"T8709","span":{"begin":226,"end":232},"obj":"PR_EXT:000016672"},{"id":"T8710","span":{"begin":233,"end":240},"obj":"CHEBI_PR_EXT:protein"},{"id":"T8711","span":{"begin":233,"end":247},"obj":"SO:0000010"},{"id":"T8712","span":{"begin":241,"end":256},"obj":"SO_EXT:coding_sequence"},{"id":"T8713","span":{"begin":276,"end":283},"obj":"SO_EXT:0000112"},{"id":"T8714","span":{"begin":454,"end":461},"obj":"SO_EXT:0000112"},{"id":"T8715","span":{"begin":481,"end":486},"obj":"PR_EXT:000010303"},{"id":"T8716","span":{"begin":487,"end":502},"obj":"SO_EXT:coding_sequence"}],"text":"Total RNA was isolated from adult testes with the RNeasy Mini Kit (Qiagen, http://www.qiagen.com), and 4.0 μg was oligo dT–primed and reverse-transcribed with Superscript II (Stratagene, http://www.stratagene.com). The entire Trip13 protein-coding sequence was amplified with primers 5′-ATGGACGAGGCGGTG-3′ and 5′-TCAAACATAAGCTGAAAGTT-3′. The cycling conditions were: 94 °C 2 min; 94 °C 30s, 55 °C 45 s, and 72 °C 80 s for 35 cycles; and 72 °C 2 min. The primers for amplifying the Med31 coding sequence as control were : 5′-ATGGCCGCGGCCGTCGCTATGG-3′ and 5′-TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC-3′. The cycling conditions were: 94 °C 2 min; 94 °C 30 s, 55 °C 30 s, and 72 °C 35 s for 35 cycles; and 72 °C 2 min."}