Id |
Subject |
Object |
Predicate |
Lexical cue |
T8793 |
361-365 |
VBD |
denotes |
were |
T8722 |
0-5 |
JJ |
denotes |
Total |
T8723 |
6-9 |
NN |
denotes |
RNA |
T8724 |
14-22 |
VBN |
denotes |
isolated |
T8725 |
10-13 |
VBD |
denotes |
was |
T8726 |
23-27 |
IN |
denotes |
from |
T8727 |
28-33 |
JJ |
denotes |
adult |
T8728 |
34-40 |
NNS |
denotes |
testes |
T8729 |
41-45 |
IN |
denotes |
with |
T8730 |
46-49 |
DT |
denotes |
the |
T8731 |
62-65 |
NNP |
denotes |
Kit |
T8732 |
50-56 |
NNP |
denotes |
RNeasy |
T8733 |
57-61 |
NNP |
denotes |
Mini |
T8734 |
66-67 |
-LRB- |
denotes |
( |
T8735 |
67-73 |
NNP |
denotes |
Qiagen |
T8736 |
73-75 |
, |
denotes |
, |
T8737 |
75-96 |
NN |
denotes |
http://www.qiagen.com |
T8738 |
96-97 |
-RRB- |
denotes |
) |
T8739 |
97-99 |
, |
denotes |
, |
T8740 |
99-102 |
CC |
denotes |
and |
T8741 |
103-106 |
CD |
denotes |
4.0 |
T8742 |
107-109 |
NN |
denotes |
μg |
T8743 |
123-129 |
VBN |
denotes |
primed |
T8744 |
110-113 |
VBD |
denotes |
was |
T8745 |
114-119 |
NN |
denotes |
oligo |
T8746 |
120-122 |
NN |
denotes |
dT |
T8747 |
122-123 |
HYPH |
denotes |
– |
T8748 |
130-133 |
CC |
denotes |
and |
T8749 |
134-141 |
RB |
denotes |
reverse |
T8750 |
142-153 |
VBN |
denotes |
transcribed |
T8751 |
141-142 |
HYPH |
denotes |
- |
T8752 |
154-158 |
IN |
denotes |
with |
T8753 |
159-170 |
NNP |
denotes |
Superscript |
T8754 |
171-173 |
CD |
denotes |
II |
T8755 |
174-175 |
-LRB- |
denotes |
( |
T8756 |
175-185 |
NNP |
denotes |
Stratagene |
T8757 |
185-187 |
, |
denotes |
, |
T8758 |
187-212 |
NN |
denotes |
http://www.stratagene.com |
T8759 |
212-213 |
-RRB- |
denotes |
) |
T8760 |
213-214 |
. |
denotes |
. |
T8761 |
214-337 |
sentence |
denotes |
The entire Trip13 protein-coding sequence was amplified with primers 5′-ATGGACGAGGCGGTG-3′ and 5′-TCAAACATAAGCTGAAAGTT-3′. |
T8762 |
215-218 |
DT |
denotes |
The |
T8763 |
248-256 |
NN |
denotes |
sequence |
T8764 |
219-225 |
JJ |
denotes |
entire |
T8765 |
226-232 |
NN |
denotes |
Trip13 |
T8766 |
233-240 |
NN |
denotes |
protein |
T8767 |
241-247 |
VBG |
denotes |
coding |
T8768 |
240-241 |
HYPH |
denotes |
- |
T8769 |
261-270 |
VBN |
denotes |
amplified |
T8770 |
257-260 |
VBD |
denotes |
was |
T8771 |
271-275 |
IN |
denotes |
with |
T8772 |
276-283 |
NNS |
denotes |
primers |
T8773 |
287-302 |
NN |
denotes |
ATGGACGAGGCGGTG |
T8774 |
284-285 |
CD |
denotes |
5 |
T8775 |
285-286 |
SYM |
denotes |
′ |
T8776 |
286-287 |
HYPH |
denotes |
- |
T8777 |
302-303 |
HYPH |
denotes |
- |
T8778 |
303-304 |
CD |
denotes |
3 |
T8779 |
304-305 |
SYM |
denotes |
′ |
T8780 |
306-309 |
CC |
denotes |
and |
T8781 |
310-311 |
CD |
denotes |
5 |
T8782 |
313-333 |
NN |
denotes |
TCAAACATAAGCTGAAAGTT |
T8783 |
311-312 |
SYM |
denotes |
′ |
T8784 |
312-313 |
HYPH |
denotes |
- |
T8785 |
333-334 |
HYPH |
denotes |
- |
T8786 |
334-335 |
CD |
denotes |
3 |
T8787 |
335-336 |
SYM |
denotes |
′ |
T8788 |
336-337 |
. |
denotes |
. |
T8789 |
337-449 |
sentence |
denotes |
The cycling conditions were: 94 °C 2 min; 94 °C 30s, 55 °C 45 s, and 72 °C 80 s for 35 cycles; and 72 °C 2 min. |
T8790 |
338-341 |
DT |
denotes |
The |
T8791 |
350-360 |
NNS |
denotes |
conditions |
T8792 |
342-349 |
NN |
denotes |
cycling |
T8794 |
365-367 |
: |
denotes |
: |
T8795 |
367-369 |
CD |
denotes |
94 |
T8796 |
370-372 |
NN |
denotes |
°C |
T8797 |
373-374 |
CD |
denotes |
2 |
T8798 |
375-378 |
NN |
denotes |
min |
T8799 |
378-379 |
: |
denotes |
; |
T8800 |
380-382 |
CD |
denotes |
94 |
T8801 |
383-385 |
NN |
denotes |
°C |
T8802 |
386-388 |
CD |
denotes |
30 |
T8803 |
388-389 |
NN |
denotes |
s |
T8804 |
389-391 |
, |
denotes |
, |
T8805 |
391-393 |
CD |
denotes |
55 |
T8806 |
394-396 |
NN |
denotes |
°C |
T8807 |
397-399 |
CD |
denotes |
45 |
T8808 |
400-401 |
NN |
denotes |
s |
T8809 |
401-403 |
, |
denotes |
, |
T8810 |
403-406 |
CC |
denotes |
and |
T8811 |
407-409 |
CD |
denotes |
72 |
T8812 |
410-412 |
NN |
denotes |
°C |
T8813 |
413-415 |
CD |
denotes |
80 |
T8814 |
416-417 |
NN |
denotes |
s |
T8815 |
418-421 |
IN |
denotes |
for |
T8816 |
422-424 |
CD |
denotes |
35 |
T8817 |
425-431 |
NNS |
denotes |
cycles |
T8818 |
431-432 |
: |
denotes |
; |
T8819 |
433-436 |
CC |
denotes |
and |
T8820 |
437-439 |
CD |
denotes |
72 |
T8821 |
440-442 |
NN |
denotes |
°C |
T8822 |
443-444 |
CD |
denotes |
2 |
T8823 |
445-448 |
NN |
denotes |
min |
T8824 |
448-449 |
. |
denotes |
. |
T8825 |
449-595 |
sentence |
denotes |
The primers for amplifying the Med31 coding sequence as control were : 5′-ATGGCCGCGGCCGTCGCTATGG-3′ and 5′-TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC-3′. |
T8826 |
450-453 |
DT |
denotes |
The |
T8827 |
454-461 |
NNS |
denotes |
primers |
T8828 |
514-518 |
VBD |
denotes |
were |
T8829 |
462-465 |
IN |
denotes |
for |
T8830 |
466-476 |
VBG |
denotes |
amplifying |
T8831 |
477-480 |
DT |
denotes |
the |
T8832 |
494-502 |
NN |
denotes |
sequence |
T8833 |
481-486 |
NN |
denotes |
Med31 |
T8834 |
487-493 |
NN |
denotes |
coding |
T8835 |
503-505 |
IN |
denotes |
as |
T8836 |
506-513 |
NN |
denotes |
control |
T8837 |
519-521 |
: |
denotes |
: |
T8838 |
521-522 |
CD |
denotes |
5 |
T8839 |
524-546 |
NN |
denotes |
ATGGCCGCGGCCGTCGCTATGG |
T8840 |
522-523 |
SYM |
denotes |
′ |
T8841 |
523-524 |
HYPH |
denotes |
- |
T8842 |
546-547 |
HYPH |
denotes |
- |
T8843 |
547-548 |
CD |
denotes |
3 |
T8844 |
548-549 |
SYM |
denotes |
′ |
T8845 |
550-553 |
CC |
denotes |
and |
T8846 |
554-555 |
CD |
denotes |
5 |
T8847 |
557-591 |
NN |
denotes |
TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC |
T8848 |
555-556 |
SYM |
denotes |
′ |
T8849 |
556-557 |
HYPH |
denotes |
- |
T8850 |
591-592 |
HYPH |
denotes |
- |
T8851 |
592-593 |
CD |
denotes |
3 |
T8852 |
593-594 |
SYM |
denotes |
′ |
T8853 |
594-595 |
. |
denotes |
. |
T8854 |
595-708 |
sentence |
denotes |
The cycling conditions were: 94 °C 2 min; 94 °C 30 s, 55 °C 30 s, and 72 °C 35 s for 35 cycles; and 72 °C 2 min. |
T8855 |
596-599 |
DT |
denotes |
The |
T8856 |
608-618 |
NNS |
denotes |
conditions |
T8857 |
600-607 |
NN |
denotes |
cycling |
T8858 |
619-623 |
VBD |
denotes |
were |
T8859 |
623-625 |
: |
denotes |
: |
T8860 |
625-627 |
CD |
denotes |
94 |
T8861 |
628-630 |
NN |
denotes |
°C |
T8862 |
631-632 |
CD |
denotes |
2 |
T8863 |
633-636 |
NN |
denotes |
min |
T8864 |
636-637 |
: |
denotes |
; |
T8865 |
638-640 |
CD |
denotes |
94 |
T8866 |
641-643 |
NN |
denotes |
°C |
T8867 |
644-646 |
CD |
denotes |
30 |
T8868 |
647-648 |
NN |
denotes |
s |
T8869 |
648-650 |
, |
denotes |
, |
T8870 |
650-652 |
CD |
denotes |
55 |
T8871 |
653-655 |
NN |
denotes |
°C |
T8872 |
656-658 |
CD |
denotes |
30 |
T8873 |
659-660 |
NN |
denotes |
s |
T8874 |
660-662 |
, |
denotes |
, |
T8875 |
662-665 |
CC |
denotes |
and |
T8876 |
666-668 |
CD |
denotes |
72 |
T8877 |
669-671 |
NN |
denotes |
°C |
T8878 |
672-674 |
CD |
denotes |
35 |
T8879 |
675-676 |
NN |
denotes |
s |
T8880 |
677-680 |
IN |
denotes |
for |
T8881 |
681-683 |
CD |
denotes |
35 |
T8882 |
684-690 |
NNS |
denotes |
cycles |
T8883 |
690-691 |
: |
denotes |
; |
T8884 |
692-695 |
CC |
denotes |
and |
T8885 |
696-698 |
CD |
denotes |
72 |
T8886 |
699-701 |
NN |
denotes |
°C |
T8887 |
702-703 |
CD |
denotes |
2 |
T8888 |
704-707 |
NN |
denotes |
min |
T8889 |
707-708 |
. |
denotes |
. |
R5873 |
T8722 |
T8723 |
amod |
Total,RNA |
R5874 |
T8723 |
T8724 |
nsubjpass |
RNA,isolated |
R5875 |
T8725 |
T8724 |
auxpass |
was,isolated |
R5876 |
T8726 |
T8724 |
prep |
from,isolated |
R5877 |
T8727 |
T8728 |
amod |
adult,testes |
R5878 |
T8728 |
T8726 |
pobj |
testes,from |
R5879 |
T8729 |
T8724 |
prep |
with,isolated |
R5880 |
T8730 |
T8731 |
det |
the,Kit |
R5881 |
T8731 |
T8729 |
pobj |
Kit,with |
R5882 |
T8732 |
T8731 |
compound |
RNeasy,Kit |
R5883 |
T8733 |
T8731 |
compound |
Mini,Kit |
R5884 |
T8734 |
T8735 |
punct |
(,Qiagen |
R5885 |
T8735 |
T8731 |
parataxis |
Qiagen,Kit |
R5886 |
T8736 |
T8735 |
punct |
", ",Qiagen |
R5887 |
T8737 |
T8735 |
npadvmod |
http://www.qiagen.com,Qiagen |
R5888 |
T8738 |
T8735 |
punct |
),Qiagen |
R5889 |
T8739 |
T8724 |
punct |
", ",isolated |
R5890 |
T8740 |
T8724 |
cc |
and,isolated |
R5891 |
T8741 |
T8742 |
nummod |
4.0,μg |
R5892 |
T8742 |
T8743 |
nsubj |
μg,primed |
R5893 |
T8743 |
T8724 |
conj |
primed,isolated |
R5894 |
T8744 |
T8743 |
aux |
was,primed |
R5895 |
T8745 |
T8746 |
compound |
oligo,dT |
R5896 |
T8746 |
T8743 |
npadvmod |
dT,primed |
R5897 |
T8747 |
T8743 |
punct |
–,primed |
R5898 |
T8748 |
T8743 |
cc |
and,primed |
R5899 |
T8749 |
T8750 |
advmod |
reverse,transcribed |
R5900 |
T8750 |
T8743 |
conj |
transcribed,primed |
R5901 |
T8751 |
T8750 |
punct |
-,transcribed |
R5902 |
T8752 |
T8743 |
prep |
with,primed |
R5903 |
T8753 |
T8752 |
pobj |
Superscript,with |
R5904 |
T8754 |
T8753 |
nummod |
II,Superscript |
R5905 |
T8755 |
T8756 |
punct |
(,Stratagene |
R5906 |
T8756 |
T8753 |
parataxis |
Stratagene,Superscript |
R5907 |
T8757 |
T8756 |
punct |
", ",Stratagene |
R5908 |
T8758 |
T8756 |
npadvmod |
http://www.stratagene.com,Stratagene |
R5909 |
T8759 |
T8756 |
punct |
),Stratagene |
R5910 |
T8760 |
T8743 |
punct |
.,primed |
R5911 |
T8762 |
T8763 |
det |
The,sequence |
R5912 |
T8763 |
T8769 |
nsubjpass |
sequence,amplified |
R5913 |
T8764 |
T8763 |
amod |
entire,sequence |
R5914 |
T8765 |
T8763 |
nmod |
Trip13,sequence |
R5915 |
T8766 |
T8767 |
npadvmod |
protein,coding |
R5916 |
T8767 |
T8763 |
amod |
coding,sequence |
R5917 |
T8768 |
T8767 |
punct |
-,coding |
R5918 |
T8770 |
T8769 |
auxpass |
was,amplified |
R5919 |
T8771 |
T8769 |
prep |
with,amplified |
R5920 |
T8772 |
T8773 |
nmod |
primers,ATGGACGAGGCGGTG |
R5921 |
T8773 |
T8771 |
pobj |
ATGGACGAGGCGGTG,with |
R5922 |
T8774 |
T8773 |
nummod |
5,ATGGACGAGGCGGTG |
R5923 |
T8775 |
T8774 |
punct |
′,5 |
R5924 |
T8776 |
T8773 |
punct |
-,ATGGACGAGGCGGTG |
R5925 |
T8777 |
T8773 |
punct |
-,ATGGACGAGGCGGTG |
R5926 |
T8778 |
T8773 |
nummod |
3,ATGGACGAGGCGGTG |
R5927 |
T8779 |
T8773 |
punct |
′,ATGGACGAGGCGGTG |
R5928 |
T8780 |
T8773 |
cc |
and,ATGGACGAGGCGGTG |
R5929 |
T8781 |
T8782 |
nummod |
5,TCAAACATAAGCTGAAAGTT |
R5930 |
T8782 |
T8773 |
conj |
TCAAACATAAGCTGAAAGTT,ATGGACGAGGCGGTG |
R5931 |
T8783 |
T8781 |
punct |
′,5 |
R5932 |
T8784 |
T8782 |
punct |
-,TCAAACATAAGCTGAAAGTT |
R5933 |
T8785 |
T8782 |
punct |
-,TCAAACATAAGCTGAAAGTT |
R5934 |
T8786 |
T8782 |
nummod |
3,TCAAACATAAGCTGAAAGTT |
R5935 |
T8787 |
T8782 |
punct |
′,TCAAACATAAGCTGAAAGTT |
R5936 |
T8788 |
T8769 |
punct |
.,amplified |
R5937 |
T8790 |
T8791 |
det |
The,conditions |
R5938 |
T8791 |
T8793 |
nsubj |
conditions,were |
R5939 |
T8792 |
T8791 |
compound |
cycling,conditions |
R5940 |
T8794 |
T8793 |
punct |
: ,were |
R5941 |
T8795 |
T8796 |
nummod |
94,°C |
R5942 |
T8796 |
T8793 |
attr |
°C,were |
R5943 |
T8797 |
T8798 |
nummod |
2,min |
R5944 |
T8798 |
T8796 |
npadvmod |
min,°C |
R5945 |
T8799 |
T8796 |
punct |
;,°C |
R5946 |
T8800 |
T8801 |
nummod |
94,°C |
R5947 |
T8801 |
T8796 |
conj |
°C,°C |
R5948 |
T8802 |
T8803 |
nummod |
30,s |
R5949 |
T8803 |
T8801 |
npadvmod |
s,°C |
R5950 |
T8804 |
T8801 |
punct |
", ",°C |
R5951 |
T8805 |
T8806 |
nummod |
55,°C |
R5952 |
T8806 |
T8801 |
conj |
°C,°C |
R5953 |
T8807 |
T8808 |
nummod |
45,s |
R5954 |
T8808 |
T8806 |
npadvmod |
s,°C |
R5955 |
T8809 |
T8806 |
punct |
", ",°C |
R5956 |
T8810 |
T8806 |
cc |
and,°C |
R5957 |
T8811 |
T8812 |
nummod |
72,°C |
R5958 |
T8812 |
T8806 |
conj |
°C,°C |
R5959 |
T8813 |
T8814 |
nummod |
80,s |
R5960 |
T8814 |
T8812 |
npadvmod |
s,°C |
R5961 |
T8815 |
T8812 |
prep |
for,°C |
R5962 |
T8816 |
T8817 |
nummod |
35,cycles |
R5963 |
T8817 |
T8815 |
pobj |
cycles,for |
R5964 |
T8818 |
T8801 |
punct |
;,°C |
R5965 |
T8819 |
T8801 |
cc |
and,°C |
R5966 |
T8820 |
T8821 |
nummod |
72,°C |
R5967 |
T8821 |
T8801 |
conj |
°C,°C |
R5968 |
T8822 |
T8823 |
nummod |
2,min |
R5969 |
T8823 |
T8821 |
npadvmod |
min,°C |
R5970 |
T8824 |
T8793 |
punct |
.,were |
R5971 |
T8826 |
T8827 |
det |
The,primers |
R5972 |
T8827 |
T8828 |
nsubj |
primers,were |
R5973 |
T8829 |
T8827 |
prep |
for,primers |
R5974 |
T8830 |
T8829 |
pcomp |
amplifying,for |
R5975 |
T8831 |
T8832 |
det |
the,sequence |
R5976 |
T8832 |
T8830 |
dobj |
sequence,amplifying |
R5977 |
T8833 |
T8832 |
compound |
Med31,sequence |
R5978 |
T8834 |
T8832 |
compound |
coding,sequence |
R5979 |
T8835 |
T8830 |
prep |
as,amplifying |
R5980 |
T8836 |
T8835 |
pobj |
control,as |
R5981 |
T8837 |
T8828 |
punct |
: ,were |
R5982 |
T8838 |
T8839 |
nummod |
5,ATGGCCGCGGCCGTCGCTATGG |
R5983 |
T8839 |
T8828 |
attr |
ATGGCCGCGGCCGTCGCTATGG,were |
R5984 |
T8840 |
T8838 |
punct |
′,5 |
R5985 |
T8841 |
T8839 |
punct |
-,ATGGCCGCGGCCGTCGCTATGG |
R5986 |
T8842 |
T8839 |
punct |
-,ATGGCCGCGGCCGTCGCTATGG |
R5987 |
T8843 |
T8839 |
nummod |
3,ATGGCCGCGGCCGTCGCTATGG |
R5988 |
T8844 |
T8839 |
punct |
′,ATGGCCGCGGCCGTCGCTATGG |
R5989 |
T8845 |
T8839 |
cc |
and,ATGGCCGCGGCCGTCGCTATGG |
R5990 |
T8846 |
T8847 |
nummod |
5,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC |
R5991 |
T8847 |
T8839 |
conj |
TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC,ATGGCCGCGGCCGTCGCTATGG |
R5992 |
T8848 |
T8846 |
punct |
′,5 |
R5993 |
T8849 |
T8847 |
punct |
-,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC |
R5994 |
T8850 |
T8847 |
punct |
-,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC |
R5995 |
T8851 |
T8847 |
nummod |
3,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC |
R5996 |
T8852 |
T8847 |
punct |
′,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC |
R5997 |
T8853 |
T8828 |
punct |
.,were |
R5998 |
T8855 |
T8856 |
det |
The,conditions |
R5999 |
T8856 |
T8858 |
nsubj |
conditions,were |
R6000 |
T8857 |
T8856 |
compound |
cycling,conditions |
R6001 |
T8859 |
T8858 |
punct |
: ,were |
R6002 |
T8860 |
T8861 |
nummod |
94,°C |
R6003 |
T8861 |
T8858 |
attr |
°C,were |
R6004 |
T8862 |
T8863 |
nummod |
2,min |
R6005 |
T8863 |
T8861 |
npadvmod |
min,°C |
R6006 |
T8864 |
T8861 |
punct |
;,°C |
R6007 |
T8865 |
T8866 |
nummod |
94,°C |
R6008 |
T8866 |
T8861 |
conj |
°C,°C |
R6009 |
T8867 |
T8868 |
nummod |
30,s |
R6010 |
T8868 |
T8866 |
npadvmod |
s,°C |
R6011 |
T8869 |
T8866 |
punct |
", ",°C |
R6012 |
T8870 |
T8871 |
nummod |
55,°C |
R6013 |
T8871 |
T8866 |
conj |
°C,°C |
R6014 |
T8872 |
T8873 |
nummod |
30,s |
R6015 |
T8873 |
T8871 |
npadvmod |
s,°C |
R6016 |
T8874 |
T8871 |
punct |
", ",°C |
R6017 |
T8875 |
T8871 |
cc |
and,°C |
R6018 |
T8876 |
T8877 |
nummod |
72,°C |
R6019 |
T8877 |
T8871 |
conj |
°C,°C |
R6020 |
T8878 |
T8879 |
nummod |
35,s |
R6021 |
T8879 |
T8877 |
npadvmod |
s,°C |
R6022 |
T8880 |
T8877 |
prep |
for,°C |
R6023 |
T8881 |
T8882 |
nummod |
35,cycles |
R6024 |
T8882 |
T8880 |
pobj |
cycles,for |
R6025 |
T8883 |
T8866 |
punct |
;,°C |
R6026 |
T8884 |
T8866 |
cc |
and,°C |
R6027 |
T8885 |
T8886 |
nummod |
72,°C |
R6028 |
T8886 |
T8866 |
conj |
°C,°C |
R6029 |
T8887 |
T8888 |
nummod |
2,min |
R6030 |
T8888 |
T8886 |
npadvmod |
min,°C |
R6031 |
T8889 |
T8858 |
punct |
.,were |