PMC:1941754 / 25458-26993 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T4933","span":{"begin":0,"end":2},"obj":"TO"},{"id":"T4934","span":{"begin":3,"end":12},"obj":"VB"},{"id":"T4935","span":{"begin":97,"end":105},"obj":"VBD"},{"id":"T4936","span":{"begin":13,"end":15},"obj":"IN"},{"id":"T4937","span":{"begin":34,"end":42},"obj":"VBZ"},{"id":"T4938","span":{"begin":16,"end":22},"obj":"NN"},{"id":"T4939","span":{"begin":23,"end":33},"obj":"NN"},{"id":"T4940","span":{"begin":43,"end":52},"obj":"NN"},{"id":"T4941","span":{"begin":53,"end":62},"obj":"VBN"},{"id":"T4942","span":{"begin":63,"end":65},"obj":"IN"},{"id":"T4943","span":{"begin":66,"end":75},"obj":"NN"},{"id":"T4944","span":{"begin":76,"end":78},"obj":"IN"},{"id":"T4945","span":{"begin":79,"end":81},"obj":"IN"},{"id":"T4946","span":{"begin":82,"end":84},"obj":"NNP"},{"id":"T4947","span":{"begin":85,"end":92},"obj":"NNP"},{"id":"T4948","span":{"begin":92,"end":94},"obj":","},{"id":"T4949","span":{"begin":94,"end":96},"obj":"PRP"},{"id":"T4950","span":{"begin":106,"end":110},"obj":"NNS"},{"id":"T4951","span":{"begin":111,"end":115},"obj":"WDT"},{"id":"T4952","span":{"begin":116,"end":120},"obj":"VBD"},{"id":"T4953","span":{"begin":121,"end":127},"obj":"RB"},{"id":"T4954","span":{"begin":128,"end":134},"obj":"NN"},{"id":"T4955","span":{"begin":135,"end":138},"obj":"IN"},{"id":"T4956","span":{"begin":139,"end":144},"obj":"NN"},{"id":"T4957","span":{"begin":145,"end":148},"obj":"CC"},{"id":"T4958","span":{"begin":149,"end":155},"obj":"NN"},{"id":"T4959","span":{"begin":155,"end":156},"obj":"."},{"id":"T4960","span":{"begin":156,"end":303},"obj":"sentence"},{"id":"T4961","span":{"begin":157,"end":162},"obj":"NN"},{"id":"T4962","span":{"begin":163,"end":165},"obj":"VBZ"},{"id":"T4963","span":{"begin":166,"end":167},"obj":"DT"},{"id":"T4964","span":{"begin":168,"end":181},"obj":"NN"},{"id":"T4965","span":{"begin":182,"end":186},"obj":"WDT"},{"id":"T4966","span":{"begin":187,"end":189},"obj":"VBZ"},{"id":"T4967","span":{"begin":190,"end":199},"obj":"JJ"},{"id":"T4968","span":{"begin":200,"end":203},"obj":"IN"},{"id":"T4969","span":{"begin":204,"end":207},"obj":"DT"},{"id":"T4970","span":{"begin":208,"end":216},"obj":"NN"},{"id":"T4971","span":{"begin":217,"end":219},"obj":"IN"},{"id":"T4972","span":{"begin":220,"end":231},"obj":"RB"},{"id":"T4973","span":{"begin":232,"end":242},"obj":"VBN"},{"id":"T4974","span":{"begin":243,"end":246},"obj":"NN"},{"id":"T4975","span":{"begin":247,"end":253},"obj":"IN"},{"id":"T4976","span":{"begin":254,"end":263},"obj":"NN"},{"id":"T4977","span":{"begin":264,"end":266},"obj":"IN"},{"id":"T4978","span":{"begin":267,"end":271},"obj":"JJ"},{"id":"T4979","span":{"begin":272,"end":281},"obj":"NNS"},{"id":"T4980","span":{"begin":281,"end":283},"obj":","},{"id":"T4981","span":{"begin":283,"end":292},"obj":"VBG"},{"id":"T4982","span":{"begin":293,"end":297},"obj":"NNS"},{"id":"T4983","span":{"begin":298,"end":299},"obj":"-LRB-"},{"id":"T4984","span":{"begin":299,"end":301},"obj":"CD"},{"id":"T4985","span":{"begin":301,"end":302},"obj":"-RRB-"},{"id":"T4986","span":{"begin":302,"end":303},"obj":"."},{"id":"T4987","span":{"begin":303,"end":425},"obj":"sentence"},{"id":"T4988","span":{"begin":304,"end":306},"obj":"IN"},{"id":"T4989","span":{"begin":341,"end":345},"obj":"VBP"},{"id":"T4990","span":{"begin":307,"end":309},"obj":"NNP"},{"id":"T4991","span":{"begin":310,"end":317},"obj":"NNP"},{"id":"T4992","span":{"begin":317,"end":319},"obj":","},{"id":"T4993","span":{"begin":319,"end":322},"obj":"NN"},{"id":"T4994","span":{"begin":333,"end":340},"obj":"NNS"},{"id":"T4995","span":{"begin":322,"end":323},"obj":"HYPH"},{"id":"T4996","span":{"begin":323,"end":325},"obj":"CD"},{"id":"T4997","span":{"begin":326,"end":332},"obj":"NN"},{"id":"T4998","span":{"begin":346,"end":355},"obj":"JJ"},{"id":"T4999","span":{"begin":356,"end":365},"obj":"NN"},{"id":"T5000","span":{"begin":365,"end":367},"obj":","},{"id":"T5001","span":{"begin":367,"end":372},"obj":"WDT"},{"id":"T5002","span":{"begin":373,"end":381},"obj":"VBZ"},{"id":"T5003","span":{"begin":382,"end":385},"obj":"NN"},{"id":"T5004","span":{"begin":388,"end":397},"obj":"JJ"},{"id":"T5005","span":{"begin":385,"end":386},"obj":"HYPH"},{"id":"T5006","span":{"begin":386,"end":387},"obj":"CD"},{"id":"T5007","span":{"begin":398,"end":407},"obj":"NN"},{"id":"T5008","span":{"begin":408,"end":410},"obj":"IN"},{"id":"T5009","span":{"begin":411,"end":420},"obj":"NN"},{"id":"T5010","span":{"begin":421,"end":422},"obj":"-LRB-"},{"id":"T5011","span":{"begin":422,"end":423},"obj":"CD"},{"id":"T5012","span":{"begin":423,"end":424},"obj":"-RRB-"},{"id":"T5013","span":{"begin":424,"end":425},"obj":"."},{"id":"T5014","span":{"begin":425,"end":628},"obj":"sentence"},{"id":"T5015","span":{"begin":426,"end":428},"obj":"IN"},{"id":"T5016","span":{"begin":459,"end":462},"obj":"VBP"},{"id":"T5017","span":{"begin":429,"end":433},"obj":"NNS"},{"id":"T5018","span":{"begin":433,"end":435},"obj":","},{"id":"T5019","span":{"begin":435,"end":440},"obj":"NN"},{"id":"T5020","span":{"begin":445,"end":458},"obj":"NNS"},{"id":"T5021","span":{"begin":441,"end":442},"obj":"SYM"},{"id":"T5022","span":{"begin":442,"end":443},"obj":"HYPH"},{"id":"T5023","span":{"begin":443,"end":444},"obj":"SYM"},{"id":"T5024","span":{"begin":463,"end":471},"obj":"RB"},{"id":"T5025","span":{"begin":472,"end":481},"obj":"JJ"},{"id":"T5026","span":{"begin":482,"end":484},"obj":"IN"},{"id":"T5027","span":{"begin":485,"end":495},"obj":"JJ"},{"id":"T5028","span":{"begin":507,"end":515},"obj":"NN"},{"id":"T5029","span":{"begin":496,"end":506},"obj":"NN"},{"id":"T5030","span":{"begin":516,"end":517},"obj":"-LRB-"},{"id":"T5031","span":{"begin":520,"end":522},"obj":"CD"},{"id":"T5032","span":{"begin":517,"end":519},"obj":"CD"},{"id":"T5033","span":{"begin":519,"end":520},"obj":","},{"id":"T5034","span":{"begin":522,"end":523},"obj":"-RRB-"},{"id":"T5035","span":{"begin":523,"end":525},"obj":","},{"id":"T5036","span":{"begin":525,"end":528},"obj":"CC"},{"id":"T5037","span":{"begin":529,"end":535},"obj":"VBP"},{"id":"T5038","span":{"begin":536,"end":540},"obj":"IN"},{"id":"T5039","span":{"begin":541,"end":552},"obj":"NNS"},{"id":"T5040","span":{"begin":553,"end":555},"obj":"IN"},{"id":"T5041","span":{"begin":556,"end":557},"obj":"DT"},{"id":"T5042","span":{"begin":558,"end":563},"obj":"NN"},{"id":"T5043","span":{"begin":564,"end":578},"obj":"JJ"},{"id":"T5044","span":{"begin":579,"end":581},"obj":"IN"},{"id":"T5045","span":{"begin":582,"end":585},"obj":"DT"},{"id":"T5046","span":{"begin":605,"end":615},"obj":"NN"},{"id":"T5047","span":{"begin":586,"end":594},"obj":"NN"},{"id":"T5048","span":{"begin":595,"end":604},"obj":"NN"},{"id":"T5049","span":{"begin":594,"end":595},"obj":"HYPH"},{"id":"T5050","span":{"begin":616,"end":617},"obj":"-LRB-"},{"id":"T5051","span":{"begin":624,"end":626},"obj":"NN"},{"id":"T5052","span":{"begin":617,"end":623},"obj":"NN"},{"id":"T5053","span":{"begin":626,"end":627},"obj":"-RRB-"},{"id":"T5054","span":{"begin":627,"end":628},"obj":"."},{"id":"T5055","span":{"begin":628,"end":826},"obj":"sentence"},{"id":"T5056","span":{"begin":629,"end":642},"obj":"NNS"},{"id":"T5057","span":{"begin":675,"end":685},"obj":"VBD"},{"id":"T5058","span":{"begin":643,"end":645},"obj":"IN"},{"id":"T5059","span":{"begin":646,"end":654},"obj":"NN"},{"id":"T5060","span":{"begin":655,"end":657},"obj":"NN"},{"id":"T5061","span":{"begin":654,"end":655},"obj":"HYPH"},{"id":"T5062","span":{"begin":668,"end":674},"obj":"NNS"},{"id":"T5063","span":{"begin":658,"end":663},"obj":"NN"},{"id":"T5064","span":{"begin":664,"end":665},"obj":"SYM"},{"id":"T5065","span":{"begin":665,"end":666},"obj":"HYPH"},{"id":"T5066","span":{"begin":666,"end":667},"obj":"SYM"},{"id":"T5067","span":{"begin":686,"end":695},"obj":"RB"},{"id":"T5068","span":{"begin":696,"end":698},"obj":"IN"},{"id":"T5069","span":{"begin":699,"end":703},"obj":"DT"},{"id":"T5070","span":{"begin":704,"end":709},"obj":"NN"},{"id":"T5071","span":{"begin":710,"end":716},"obj":"IN"},{"id":"T5072","span":{"begin":717,"end":727},"obj":"VBG"},{"id":"T5073","span":{"begin":728,"end":733},"obj":"NN"},{"id":"T5074","span":{"begin":734,"end":735},"obj":"-LRB-"},{"id":"T5075","span":{"begin":740,"end":742},"obj":"NN"},{"id":"T5076","span":{"begin":735,"end":739},"obj":"NNS"},{"id":"T5077","span":{"begin":742,"end":743},"obj":"-RRB-"},{"id":"T5078","span":{"begin":743,"end":745},"obj":","},{"id":"T5079","span":{"begin":745,"end":749},"obj":"RB"},{"id":"T5080","span":{"begin":750,"end":756},"obj":"IN"},{"id":"T5081","span":{"begin":757,"end":760},"obj":"DT"},{"id":"T5082","span":{"begin":769,"end":779},"obj":"NN"},{"id":"T5083","span":{"begin":761,"end":768},"obj":"NN"},{"id":"T5084","span":{"begin":780,"end":784},"obj":"WDT"},{"id":"T5085","span":{"begin":785,"end":795},"obj":"VBZ"},{"id":"T5086","span":{"begin":796,"end":806},"obj":"NN"},{"id":"T5087","span":{"begin":807,"end":820},"obj":"NNS"},{"id":"T5088","span":{"begin":821,"end":822},"obj":"-LRB-"},{"id":"T5089","span":{"begin":822,"end":824},"obj":"CD"},{"id":"T5090","span":{"begin":824,"end":825},"obj":"-RRB-"},{"id":"T5091","span":{"begin":825,"end":826},"obj":"."},{"id":"T5092","span":{"begin":826,"end":976},"obj":"sentence"},{"id":"T5093","span":{"begin":827,"end":832},"obj":"EX"},{"id":"T5094","span":{"begin":833,"end":836},"obj":"VBD"},{"id":"T5095","span":{"begin":837,"end":839},"obj":"DT"},{"id":"T5096","span":{"begin":840,"end":848},"obj":"NN"},{"id":"T5097","span":{"begin":849,"end":851},"obj":"IN"},{"id":"T5098","span":{"begin":852,"end":861},"obj":"NN"},{"id":"T5099","span":{"begin":864,"end":877},"obj":"NNS"},{"id":"T5100","span":{"begin":862,"end":863},"obj":"CD"},{"id":"T5101","span":{"begin":878,"end":880},"obj":"CC"},{"id":"T5102","span":{"begin":881,"end":892},"obj":"JJ"},{"id":"T5103","span":{"begin":893,"end":903},"obj":"NNS"},{"id":"T5104","span":{"begin":904,"end":906},"obj":"IN"},{"id":"T5105","span":{"begin":907,"end":912},"obj":"DT"},{"id":"T5106","span":{"begin":913,"end":919},"obj":"NNS"},{"id":"T5107","span":{"begin":919,"end":921},"obj":","},{"id":"T5108","span":{"begin":921,"end":927},"obj":"IN"},{"id":"T5109","span":{"begin":928,"end":933},"obj":"DT"},{"id":"T5110","span":{"begin":934,"end":938},"obj":"VBN"},{"id":"T5111","span":{"begin":939,"end":941},"obj":"IN"},{"id":"T5112","span":{"begin":942,"end":948},"obj":"NN"},{"id":"T5113","span":{"begin":956,"end":963},"obj":"NNS"},{"id":"T5114","span":{"begin":949,"end":955},"obj":"JJ"},{"id":"T5115","span":{"begin":964,"end":965},"obj":"-LRB-"},{"id":"T5116","span":{"begin":972,"end":974},"obj":"NN"},{"id":"T5117","span":{"begin":965,"end":971},"obj":"NN"},{"id":"T5118","span":{"begin":974,"end":975},"obj":"-RRB-"},{"id":"T5119","span":{"begin":975,"end":976},"obj":"."},{"id":"T5120","span":{"begin":976,"end":1316},"obj":"sentence"},{"id":"T5121","span":{"begin":977,"end":979},"obj":"IN"},{"id":"T5122","span":{"begin":1260,"end":1269},"obj":"VBN"},{"id":"T5123","span":{"begin":980,"end":988},"obj":"NN"},{"id":"T5124","span":{"begin":989,"end":991},"obj":"IN"},{"id":"T5125","span":{"begin":992,"end":995},"obj":"DT"},{"id":"T5126","span":{"begin":1005,"end":1013},"obj":"NN"},{"id":"T5127","span":{"begin":996,"end":1004},"obj":"JJ"},{"id":"T5128","span":{"begin":1014,"end":1016},"obj":"IN"},{"id":"T5129","span":{"begin":1017,"end":1025},"obj":"NN"},{"id":"T5130","span":{"begin":1026,"end":1028},"obj":"NN"},{"id":"T5131","span":{"begin":1025,"end":1026},"obj":"HYPH"},{"id":"T5132","span":{"begin":1029,"end":1038},"obj":"NN"},{"id":"T5133","span":{"begin":1039,"end":1052},"obj":"NNS"},{"id":"T5134","span":{"begin":1053,"end":1054},"obj":"-LRB-"},{"id":"T5135","span":{"begin":1061,"end":1063},"obj":"NN"},{"id":"T5136","span":{"begin":1054,"end":1060},"obj":"NN"},{"id":"T5137","span":{"begin":1063,"end":1064},"obj":"-RRB-"},{"id":"T5138","span":{"begin":1064,"end":1066},"obj":","},{"id":"T5139","span":{"begin":1066,"end":1068},"obj":"IN"},{"id":"T5140","span":{"begin":1081,"end":1083},"obj":"VBZ"},{"id":"T5141","span":{"begin":1069,"end":1074},"obj":"WDT"},{"id":"T5142","span":{"begin":1075,"end":1080},"obj":"NN"},{"id":"T5143","span":{"begin":1084,"end":1093},"obj":"JJ"},{"id":"T5144","span":{"begin":1094,"end":1096},"obj":"IN"},{"id":"T5145","span":{"begin":1097,"end":1106},"obj":"NN"},{"id":"T5146","span":{"begin":1107,"end":1109},"obj":"TO"},{"id":"T5147","span":{"begin":1110,"end":1118},"obj":"VB"},{"id":"T5148","span":{"begin":1119,"end":1120},"obj":"-LRB-"},{"id":"T5149","span":{"begin":1146,"end":1149},"obj":"NN"},{"id":"T5150","span":{"begin":1120,"end":1123},"obj":"IN"},{"id":"T5151","span":{"begin":1124,"end":1127},"obj":"NN"},{"id":"T5152","span":{"begin":1128,"end":1137},"obj":"NN"},{"id":"T5153","span":{"begin":1137,"end":1139},"obj":","},{"id":"T5154","span":{"begin":1139,"end":1145},"obj":"NN"},{"id":"T5155","span":{"begin":1150,"end":1153},"obj":"CC"},{"id":"T5156","span":{"begin":1154,"end":1157},"obj":"NN"},{"id":"T5157","span":{"begin":1157,"end":1158},"obj":"-RRB-"},{"id":"T5158","span":{"begin":1159,"end":1160},"obj":"DT"},{"id":"T5159","span":{"begin":1190,"end":1196},"obj":"NN"},{"id":"T5160","span":{"begin":1161,"end":1174},"obj":"NN"},{"id":"T5161","span":{"begin":1175,"end":1181},"obj":"VBN"},{"id":"T5162","span":{"begin":1174,"end":1175},"obj":"HYPH"},{"id":"T5163","span":{"begin":1182,"end":1189},"obj":"NN"},{"id":"T5164","span":{"begin":1196,"end":1198},"obj":","},{"id":"T5165","span":{"begin":1198,"end":1208},"obj":"NN"},{"id":"T5166","span":{"begin":1209,"end":1217},"obj":"NN"},{"id":"T5167","span":{"begin":1218,"end":1220},"obj":"IN"},{"id":"T5168","span":{"begin":1221,"end":1227},"obj":"RB"},{"id":"T5169","span":{"begin":1228,"end":1234},"obj":"JJ"},{"id":"T5170","span":{"begin":1235,"end":1248},"obj":"NNS"},{"id":"T5171","span":{"begin":1249,"end":1252},"obj":"VBD"},{"id":"T5172","span":{"begin":1253,"end":1259},"obj":"RB"},{"id":"T5173","span":{"begin":1270,"end":1272},"obj":"IN"},{"id":"T5174","span":{"begin":1273,"end":1275},"obj":"IN"},{"id":"T5175","span":{"begin":1276,"end":1281},"obj":"NN"},{"id":"T5176","span":{"begin":1289,"end":1296},"obj":"NNS"},{"id":"T5177","span":{"begin":1282,"end":1288},"obj":"JJ"},{"id":"T5178","span":{"begin":1297,"end":1298},"obj":"-LRB-"},{"id":"T5179","span":{"begin":1305,"end":1307},"obj":"NN"},{"id":"T5180","span":{"begin":1298,"end":1304},"obj":"NN"},{"id":"T5181","span":{"begin":1308,"end":1311},"obj":"CC"},{"id":"T5182","span":{"begin":1312,"end":1314},"obj":"NN"},{"id":"T5183","span":{"begin":1314,"end":1315},"obj":"-RRB-"},{"id":"T5184","span":{"begin":1315,"end":1316},"obj":"."},{"id":"T5185","span":{"begin":1316,"end":1535},"obj":"sentence"},{"id":"T5186","span":{"begin":1317,"end":1326},"obj":"JJ"},{"id":"T5187","span":{"begin":1327,"end":1334},"obj":"NNS"},{"id":"T5188","span":{"begin":1340,"end":1349},"obj":"VBN"},{"id":"T5189","span":{"begin":1335,"end":1339},"obj":"VBD"},{"id":"T5190","span":{"begin":1350,"end":1354},"obj":"IN"},{"id":"T5191","span":{"begin":1355,"end":1359},"obj":"NNS"},{"id":"T5192","span":{"begin":1360,"end":1369},"obj":"JJ"},{"id":"T5198","span":{"begin":1382,"end":1392},"obj":"NN"},{"id":"T5199","span":{"begin":1393,"end":1401},"obj":"JJ"},{"id":"T5200","span":{"begin":1392,"end":1393},"obj":"HYPH"},{"id":"T5201","span":{"begin":1407,"end":1411},"obj":"RB"},{"id":"T5202","span":{"begin":1412,"end":1420},"obj":"VBN"},{"id":"T5203","span":{"begin":1421,"end":1424},"obj":"IN"},{"id":"T5204","span":{"begin":1425,"end":1428},"obj":"NN"},{"id":"T5205","span":{"begin":1429,"end":1438},"obj":"NN"},{"id":"T5206","span":{"begin":1439,"end":1442},"obj":"CC"},{"id":"T5207","span":{"begin":1443,"end":1453},"obj":"NN"},{"id":"T5208","span":{"begin":1454,"end":1462},"obj":"NN"},{"id":"T5209","span":{"begin":1463,"end":1464},"obj":"-LRB-"},{"id":"T5210","span":{"begin":1464,"end":1466},"obj":"CD"},{"id":"T5211","span":{"begin":1466,"end":1467},"obj":"-RRB-"},{"id":"T5212","span":{"begin":1467,"end":1469},"obj":","},{"id":"T5213","span":{"begin":1469,"end":1473},"obj":"IN"},{"id":"T5193","span":{"begin":1370,"end":1373},"obj":"IN"},{"id":"T5194","span":{"begin":1374,"end":1378},"obj":"NN"},{"id":"T5195","span":{"begin":1378,"end":1380},"obj":","},{"id":"T5196","span":{"begin":1380,"end":1381},"obj":"DT"},{"id":"T5197","span":{"begin":1402,"end":1406},"obj":"NN"},{"id":"T5214","span":{"begin":1474,"end":1481},"obj":"JJ"},{"id":"T5215","span":{"begin":1482,"end":1489},"obj":"NNS"},{"id":"T5216","span":{"begin":1490,"end":1491},"obj":"-LRB-"},{"id":"T5217","span":{"begin":1498,"end":1500},"obj":"NN"},{"id":"T5218","span":{"begin":1491,"end":1497},"obj":"NN"},{"id":"T5219","span":{"begin":1501,"end":1504},"obj":"CC"},{"id":"T5220","span":{"begin":1505,"end":1507},"obj":"NN"},{"id":"T5221","span":{"begin":1507,"end":1508},"obj":":"},{"id":"T5222","span":{"begin":1509,"end":1523},"obj":"NN"},{"id":"T5223","span":{"begin":1528,"end":1533},"obj":"VBN"},{"id":"T5224","span":{"begin":1524,"end":1527},"obj":"RB"},{"id":"T5225","span":{"begin":1533,"end":1534},"obj":"-RRB-"},{"id":"T5226","span":{"begin":1534,"end":1535},"obj":"."}],"relations":[{"id":"R3083","pred":"aux","subj":"T4933","obj":"T4934"},{"id":"R3084","pred":"advcl","subj":"T4934","obj":"T4935"},{"id":"R3085","pred":"mark","subj":"T4936","obj":"T4937"},{"id":"R3086","pred":"ccomp","subj":"T4937","obj":"T4934"},{"id":"R3087","pred":"compound","subj":"T4938","obj":"T4939"},{"id":"R3088","pred":"nsubj","subj":"T4939","obj":"T4937"},{"id":"R3089","pred":"dobj","subj":"T4940","obj":"T4937"},{"id":"R3090","pred":"acl","subj":"T4941","obj":"T4940"},{"id":"R3091","pred":"agent","subj":"T4942","obj":"T4941"},{"id":"R3092","pred":"pobj","subj":"T4943","obj":"T4942"},{"id":"R3093","pred":"prep","subj":"T4944","obj":"T4941"},{"id":"R3094","pred":"prep","subj":"T4945","obj":"T4944"},{"id":"R3095","pred":"compound","subj":"T4946","obj":"T4947"},{"id":"R3096","pred":"pobj","subj":"T4947","obj":"T4945"},{"id":"R3097","pred":"punct","subj":"T4948","obj":"T4935"},{"id":"R3098","pred":"nsubj","subj":"T4949","obj":"T4935"},{"id":"R3099","pred":"dobj","subj":"T4950","obj":"T4935"},{"id":"R3100","pred":"dep","subj":"T4951","obj":"T4952"},{"id":"R3101","pred":"relcl","subj":"T4952","obj":"T4950"},{"id":"R3102","pred":"advmod","subj":"T4953","obj":"T4954"},{"id":"R3103","pred":"acomp","subj":"T4954","obj":"T4952"},{"id":"R3104","pred":"prep","subj":"T4955","obj":"T4954"},{"id":"R3105","pred":"pobj","subj":"T4956","obj":"T4955"},{"id":"R3106","pred":"cc","subj":"T4957","obj":"T4956"},{"id":"R3107","pred":"conj","subj":"T4958","obj":"T4956"},{"id":"R3108","pred":"punct","subj":"T4959","obj":"T4935"},{"id":"R3109","pred":"nsubj","subj":"T4961","obj":"T4962"},{"id":"R3110","pred":"det","subj":"T4963","obj":"T4964"},{"id":"R3111","pred":"attr","subj":"T4964","obj":"T4962"},{"id":"R3112","pred":"dep","subj":"T4965","obj":"T4966"},{"id":"R3113","pred":"relcl","subj":"T4966","obj":"T4964"},{"id":"R3114","pred":"acomp","subj":"T4967","obj":"T4966"},{"id":"R3115","pred":"prep","subj":"T4968","obj":"T4967"},{"id":"R3116","pred":"det","subj":"T4969","obj":"T4970"},{"id":"R3117","pred":"pobj","subj":"T4970","obj":"T4968"},{"id":"R3118","pred":"prep","subj":"T4971","obj":"T4970"},{"id":"R3119","pred":"advmod","subj":"T4972","obj":"T4973"},{"id":"R3120","pred":"amod","subj":"T4973","obj":"T4974"},{"id":"R3121","pred":"pobj","subj":"T4974","obj":"T4971"},{"id":"R3122","pred":"prep","subj":"T4975","obj":"T4970"},{"id":"R3123","pred":"pobj","subj":"T4976","obj":"T4975"},{"id":"R3124","pred":"prep","subj":"T4977","obj":"T4976"},{"id":"R3125","pred":"amod","subj":"T4978","obj":"T4979"},{"id":"R3126","pred":"pobj","subj":"T4979","obj":"T4977"},{"id":"R3127","pred":"punct","subj":"T4980","obj":"T4979"},{"id":"R3128","pred":"prep","subj":"T4981","obj":"T4979"},{"id":"R3129","pred":"pobj","subj":"T4982","obj":"T4981"},{"id":"R3130","pred":"punct","subj":"T4983","obj":"T4984"},{"id":"R3131","pred":"parataxis","subj":"T4984","obj":"T4966"},{"id":"R3132","pred":"punct","subj":"T4985","obj":"T4984"},{"id":"R3133","pred":"punct","subj":"T4986","obj":"T4962"},{"id":"R3134","pred":"prep","subj":"T4988","obj":"T4989"},{"id":"R3135","pred":"compound","subj":"T4990","obj":"T4991"},{"id":"R3136","pred":"pobj","subj":"T4991","obj":"T4988"},{"id":"R3137","pred":"punct","subj":"T4992","obj":"T4989"},{"id":"R3138","pred":"nmod","subj":"T4993","obj":"T4994"},{"id":"R3139","pred":"nsubj","subj":"T4994","obj":"T4989"},{"id":"R3140","pred":"punct","subj":"T4995","obj":"T4993"},{"id":"R3141","pred":"nummod","subj":"T4996","obj":"T4993"},{"id":"R3142","pred":"compound","subj":"T4997","obj":"T4994"},{"id":"R3143","pred":"amod","subj":"T4998","obj":"T4999"},{"id":"R3144","pred":"dobj","subj":"T4999","obj":"T4989"},{"id":"R3145","pred":"punct","subj":"T5000","obj":"T4999"},{"id":"R3146","pred":"dep","subj":"T5001","obj":"T5002"},{"id":"R3147","pred":"relcl","subj":"T5002","obj":"T4999"},{"id":"R3148","pred":"npadvmod","subj":"T5003","obj":"T5004"},{"id":"R3149","pred":"amod","subj":"T5004","obj":"T5007"},{"id":"R3150","pred":"punct","subj":"T5005","obj":"T5003"},{"id":"R3151","pred":"nummod","subj":"T5006","obj":"T5003"},{"id":"R3152","pred":"dobj","subj":"T5007","obj":"T5002"},{"id":"R3153","pred":"prep","subj":"T5008","obj":"T5007"},{"id":"R3154","pred":"pobj","subj":"T5009","obj":"T5008"},{"id":"R3155","pred":"punct","subj":"T5010","obj":"T5011"},{"id":"R3156","pred":"parataxis","subj":"T5011","obj":"T4989"},{"id":"R3157","pred":"punct","subj":"T5012","obj":"T5011"},{"id":"R3158","pred":"punct","subj":"T5013","obj":"T4989"},{"id":"R3159","pred":"prep","subj":"T5015","obj":"T5016"},{"id":"R3160","pred":"pobj","subj":"T5017","obj":"T5015"},{"id":"R3161","pred":"punct","subj":"T5018","obj":"T5016"},{"id":"R3162","pred":"nmod","subj":"T5019","obj":"T5020"},{"id":"R3163","pred":"nsubj","subj":"T5020","obj":"T5016"},{"id":"R3164","pred":"punct","subj":"T5021","obj":"T5019"},{"id":"R3165","pred":"punct","subj":"T5022","obj":"T5019"},{"id":"R3166","pred":"punct","subj":"T5023","obj":"T5019"},{"id":"R3167","pred":"advmod","subj":"T5024","obj":"T5025"},{"id":"R3168","pred":"acomp","subj":"T5025","obj":"T5016"},{"id":"R3169","pred":"prep","subj":"T5026","obj":"T5016"},{"id":"R3170","pred":"amod","subj":"T5027","obj":"T5028"},{"id":"R3171","pred":"pobj","subj":"T5028","obj":"T5026"},{"id":"R3172","pred":"compound","subj":"T5029","obj":"T5028"},{"id":"R3173","pred":"punct","subj":"T5030","obj":"T5031"},{"id":"R3174","pred":"parataxis","subj":"T5031","obj":"T5016"},{"id":"R3175","pred":"nummod","subj":"T5032","obj":"T5031"},{"id":"R3176","pred":"punct","subj":"T5033","obj":"T5031"},{"id":"R3177","pred":"punct","subj":"T5034","obj":"T5031"},{"id":"R3178","pred":"punct","subj":"T5035","obj":"T5016"},{"id":"R3179","pred":"cc","subj":"T5036","obj":"T5016"},{"id":"R3180","pred":"conj","subj":"T5037","obj":"T5016"},{"id":"R3181","pred":"prep","subj":"T5038","obj":"T5037"},{"id":"R3182","pred":"pobj","subj":"T5039","obj":"T5038"},{"id":"R3183","pred":"prep","subj":"T5040","obj":"T5037"},{"id":"R3184","pred":"det","subj":"T5041","obj":"T5042"},{"id":"R3185","pred":"pobj","subj":"T5042","obj":"T5040"},{"id":"R3186","pred":"amod","subj":"T5043","obj":"T5042"},{"id":"R3187","pred":"prep","subj":"T5044","obj":"T5043"},{"id":"R3188","pred":"det","subj":"T5045","obj":"T5046"},{"id":"R3189","pred":"pobj","subj":"T5046","obj":"T5044"},{"id":"R3190","pred":"compound","subj":"T5047","obj":"T5048"},{"id":"R3191","pred":"compound","subj":"T5048","obj":"T5046"},{"id":"R3192","pred":"punct","subj":"T5049","obj":"T5048"},{"id":"R3193","pred":"punct","subj":"T5050","obj":"T5051"},{"id":"R3194","pred":"parataxis","subj":"T5051","obj":"T5037"},{"id":"R3195","pred":"compound","subj":"T5052","obj":"T5051"},{"id":"R3196","pred":"punct","subj":"T5053","obj":"T5051"},{"id":"R3197","pred":"punct","subj":"T5054","obj":"T5016"},{"id":"R3198","pred":"nsubj","subj":"T5056","obj":"T5057"},{"id":"R3199","pred":"prep","subj":"T5058","obj":"T5056"},{"id":"R3200","pred":"nmod","subj":"T5059","obj":"T5060"},{"id":"R3201","pred":"nmod","subj":"T5060","obj":"T5062"},{"id":"R3202","pred":"punct","subj":"T5061","obj":"T5060"},{"id":"R3203","pred":"pobj","subj":"T5062","obj":"T5058"},{"id":"R3204","pred":"nmod","subj":"T5063","obj":"T5062"},{"id":"R3205","pred":"punct","subj":"T5064","obj":"T5063"},{"id":"R3206","pred":"punct","subj":"T5065","obj":"T5063"},{"id":"R3207","pred":"punct","subj":"T5066","obj":"T5063"},{"id":"R3208","pred":"advmod","subj":"T5067","obj":"T5057"},{"id":"R3209","pred":"prep","subj":"T5068","obj":"T5057"},{"id":"R3210","pred":"det","subj":"T5069","obj":"T5070"},{"id":"R3211","pred":"pobj","subj":"T5070","obj":"T5068"},{"id":"R3212","pred":"prep","subj":"T5071","obj":"T5057"},{"id":"R3213","pred":"pcomp","subj":"T5072","obj":"T5071"},{"id":"R3214","pred":"dobj","subj":"T5073","obj":"T5072"},{"id":"R3215","pred":"punct","subj":"T5074","obj":"T5075"},{"id":"R3216","pred":"parataxis","subj":"T5075","obj":"T5072"},{"id":"R3217","pred":"compound","subj":"T5076","obj":"T5075"},{"id":"R3218","pred":"punct","subj":"T5077","obj":"T5075"},{"id":"R3219","pred":"punct","subj":"T5078","obj":"T5072"},{"id":"R3220","pred":"advmod","subj":"T5079","obj":"T5080"},{"id":"R3221","pred":"prep","subj":"T5080","obj":"T5072"},{"id":"R3222","pred":"det","subj":"T5081","obj":"T5082"},{"id":"R3223","pred":"pobj","subj":"T5082","obj":"T5080"},{"id":"R3224","pred":"compound","subj":"T5083","obj":"T5082"},{"id":"R3225","pred":"dep","subj":"T5084","obj":"T5085"},{"id":"R3226","pred":"relcl","subj":"T5085","obj":"T5082"},{"id":"R3227","pred":"compound","subj":"T5086","obj":"T5087"},{"id":"R3228","pred":"dobj","subj":"T5087","obj":"T5085"},{"id":"R3229","pred":"punct","subj":"T5088","obj":"T5089"},{"id":"R3230","pred":"parataxis","subj":"T5089","obj":"T5057"},{"id":"R3231","pred":"punct","subj":"T5090","obj":"T5089"},{"id":"R3232","pred":"punct","subj":"T5091","obj":"T5057"},{"id":"R3233","pred":"expl","subj":"T5093","obj":"T5094"},{"id":"R3234","pred":"det","subj":"T5095","obj":"T5096"},{"id":"R3235","pred":"attr","subj":"T5096","obj":"T5094"},{"id":"R3236","pred":"prep","subj":"T5097","obj":"T5096"},{"id":"R3237","pred":"nmod","subj":"T5098","obj":"T5099"},{"id":"R3238","pred":"pobj","subj":"T5099","obj":"T5097"},{"id":"R3239","pred":"nummod","subj":"T5100","obj":"T5098"},{"id":"R3240","pred":"cc","subj":"T5101","obj":"T5099"},{"id":"R3241","pred":"amod","subj":"T5102","obj":"T5103"},{"id":"R3242","pred":"conj","subj":"T5103","obj":"T5099"},{"id":"R3243","pred":"prep","subj":"T5104","obj":"T5096"},{"id":"R3244","pred":"det","subj":"T5105","obj":"T5106"},{"id":"R3245","pred":"pobj","subj":"T5106","obj":"T5104"},{"id":"R3246","pred":"punct","subj":"T5107","obj":"T5094"},{"id":"R3247","pred":"prep","subj":"T5108","obj":"T5094"},{"id":"R3248","pred":"pobj","subj":"T5109","obj":"T5108"},{"id":"R3249","pred":"acl","subj":"T5110","obj":"T5109"},{"id":"R3250","pred":"prep","subj":"T5111","obj":"T5110"},{"id":"R3251","pred":"nmod","subj":"T5112","obj":"T5113"},{"id":"R3252","pred":"pobj","subj":"T5113","obj":"T5111"},{"id":"R3253","pred":"amod","subj":"T5114","obj":"T5113"},{"id":"R3254","pred":"punct","subj":"T5115","obj":"T5116"},{"id":"R3255","pred":"parataxis","subj":"T5116","obj":"T5094"},{"id":"R3256","pred":"compound","subj":"T5117","obj":"T5116"},{"id":"R3257","pred":"punct","subj":"T5118","obj":"T5116"},{"id":"R3258","pred":"punct","subj":"T5119","obj":"T5094"},{"id":"R3259","pred":"prep","subj":"T5121","obj":"T5122"},{"id":"R3260","pred":"pobj","subj":"T5123","obj":"T5121"},{"id":"R3261","pred":"prep","subj":"T5124","obj":"T5123"},{"id":"R3262","pred":"det","subj":"T5125","obj":"T5126"},{"id":"R3263","pred":"pobj","subj":"T5126","obj":"T5124"},{"id":"R3264","pred":"amod","subj":"T5127","obj":"T5126"},{"id":"R3265","pred":"prep","subj":"T5128","obj":"T5126"},{"id":"R3266","pred":"compound","subj":"T5129","obj":"T5130"},{"id":"R3267","pred":"compound","subj":"T5130","obj":"T5132"},{"id":"R3268","pred":"punct","subj":"T5131","obj":"T5130"},{"id":"R3269","pred":"compound","subj":"T5132","obj":"T5133"},{"id":"R3270","pred":"pobj","subj":"T5133","obj":"T5128"},{"id":"R3271","pred":"punct","subj":"T5134","obj":"T5135"},{"id":"R3272","pred":"parataxis","subj":"T5135","obj":"T5126"},{"id":"R3273","pred":"compound","subj":"T5136","obj":"T5135"},{"id":"R3274","pred":"punct","subj":"T5137","obj":"T5135"},{"id":"R3275","pred":"punct","subj":"T5138","obj":"T5126"},{"id":"R3276","pred":"prep","subj":"T5139","obj":"T5140"},{"id":"R3277","pred":"relcl","subj":"T5140","obj":"T5126"},{"id":"R3278","pred":"pobj","subj":"T5141","obj":"T5139"},{"id":"R3279","pred":"nsubj","subj":"T5142","obj":"T5140"},{"id":"R3280","pred":"acomp","subj":"T5143","obj":"T5140"},{"id":"R3281","pred":"prep","subj":"T5144","obj":"T5140"},{"id":"R3282","pred":"pobj","subj":"T5145","obj":"T5144"},{"id":"R3283","pred":"aux","subj":"T5146","obj":"T5147"},{"id":"R3284","pred":"advcl","subj":"T5147","obj":"T5140"},{"id":"R3285","pred":"punct","subj":"T5148","obj":"T5149"},{"id":"R3286","pred":"parataxis","subj":"T5149","obj":"T5147"},{"id":"R3287","pred":"prep","subj":"T5150","obj":"T5149"},{"id":"R3288","pred":"compound","subj":"T5151","obj":"T5152"},{"id":"R3289","pred":"pobj","subj":"T5152","obj":"T5150"},{"id":"R3290","pred":"punct","subj":"T5153","obj":"T5149"},{"id":"R3291","pred":"compound","subj":"T5154","obj":"T5149"},{"id":"R3292","pred":"cc","subj":"T5155","obj":"T5149"},{"id":"R3293","pred":"conj","subj":"T5156","obj":"T5149"},{"id":"R3294","pred":"punct","subj":"T5157","obj":"T5149"},{"id":"R3295","pred":"det","subj":"T5158","obj":"T5159"},{"id":"R3296","pred":"dobj","subj":"T5159","obj":"T5147"},{"id":"R3297","pred":"npadvmod","subj":"T5160","obj":"T5161"},{"id":"R3298","pred":"amod","subj":"T5161","obj":"T5159"},{"id":"R3299","pred":"punct","subj":"T5162","obj":"T5161"},{"id":"R3300","pred":"compound","subj":"T5163","obj":"T5159"},{"id":"R3301","pred":"punct","subj":"T5164","obj":"T5122"},{"id":"R3302","pred":"compound","subj":"T5165","obj":"T5166"},{"id":"R3303","pred":"nsubjpass","subj":"T5166","obj":"T5122"},{"id":"R3304","pred":"prep","subj":"T5167","obj":"T5166"},{"id":"R3305","pred":"advmod","subj":"T5168","obj":"T5169"},{"id":"R3306","pred":"amod","subj":"T5169","obj":"T5170"},{"id":"R3307","pred":"pobj","subj":"T5170","obj":"T5167"},{"id":"R3308","pred":"auxpass","subj":"T5171","obj":"T5122"},{"id":"R3309","pred":"advmod","subj":"T5172","obj":"T5122"},{"id":"R3310","pred":"prep","subj":"T5173","obj":"T5122"},{"id":"R3311","pred":"prep","subj":"T5174","obj":"T5173"},{"id":"R3312","pred":"nmod","subj":"T5175","obj":"T5176"},{"id":"R3313","pred":"pobj","subj":"T5176","obj":"T5174"},{"id":"R3314","pred":"amod","subj":"T5177","obj":"T5176"},{"id":"R3315","pred":"punct","subj":"T5178","obj":"T5179"},{"id":"R3317","pred":"compound","subj":"T5180","obj":"T5179"},{"id":"R3346","pred":"punct","subj":"T5211","obj":"T5210"},{"id":"R3347","pred":"punct","subj":"T5212","obj":"T5188"},{"id":"R3348","pred":"prep","subj":"T5213","obj":"T5188"},{"id":"R3349","pred":"amod","subj":"T5214","obj":"T5215"},{"id":"R3350","pred":"pobj","subj":"T5215","obj":"T5213"},{"id":"R3351","pred":"punct","subj":"T5216","obj":"T5217"},{"id":"R3352","pred":"parataxis","subj":"T5217","obj":"T5188"},{"id":"R3353","pred":"compound","subj":"T5218","obj":"T5217"},{"id":"R3354","pred":"cc","subj":"T5219","obj":"T5217"},{"id":"R3355","pred":"conj","subj":"T5220","obj":"T5217"},{"id":"R3356","pred":"punct","subj":"T5221","obj":"T5217"},{"id":"R3357","pred":"nsubj","subj":"T5222","obj":"T5223"},{"id":"R3358","pred":"ccomp","subj":"T5223","obj":"T5217"},{"id":"R3359","pred":"neg","subj":"T5224","obj":"T5223"},{"id":"R3360","pred":"punct","subj":"T5225","obj":"T5217"},{"id":"R3361","pred":"punct","subj":"T5226","obj":"T5188"},{"id":"R3316","pred":"parataxis","subj":"T5179","obj":"T5122"},{"id":"R3318","pred":"cc","subj":"T5181","obj":"T5179"},{"id":"R3319","pred":"conj","subj":"T5182","obj":"T5179"},{"id":"R3320","pred":"punct","subj":"T5183","obj":"T5179"},{"id":"R3321","pred":"punct","subj":"T5184","obj":"T5122"},{"id":"R3322","pred":"amod","subj":"T5186","obj":"T5187"},{"id":"R3323","pred":"nsubjpass","subj":"T5187","obj":"T5188"},{"id":"R3324","pred":"auxpass","subj":"T5189","obj":"T5188"},{"id":"R3325","pred":"prep","subj":"T5190","obj":"T5188"},{"id":"R3326","pred":"pobj","subj":"T5191","obj":"T5190"},{"id":"R3327","pred":"amod","subj":"T5192","obj":"T5191"},{"id":"R3328","pred":"prep","subj":"T5193","obj":"T5192"},{"id":"R3329","pred":"pobj","subj":"T5194","obj":"T5193"},{"id":"R3330","pred":"punct","subj":"T5195","obj":"T5194"},{"id":"R3331","pred":"det","subj":"T5196","obj":"T5197"},{"id":"R3332","pred":"appos","subj":"T5197","obj":"T5194"},{"id":"R3333","pred":"npadvmod","subj":"T5198","obj":"T5199"},{"id":"R3334","pred":"amod","subj":"T5199","obj":"T5197"},{"id":"R3335","pred":"punct","subj":"T5200","obj":"T5199"},{"id":"R3336","pred":"advmod","subj":"T5201","obj":"T5202"},{"id":"R3337","pred":"acl","subj":"T5202","obj":"T5197"},{"id":"R3338","pred":"prep","subj":"T5203","obj":"T5202"},{"id":"R3339","pred":"compound","subj":"T5204","obj":"T5205"},{"id":"R3340","pred":"pobj","subj":"T5205","obj":"T5203"},{"id":"R3341","pred":"cc","subj":"T5206","obj":"T5205"},{"id":"R3342","pred":"compound","subj":"T5207","obj":"T5208"},{"id":"R3343","pred":"conj","subj":"T5208","obj":"T5205"},{"id":"R3344","pred":"punct","subj":"T5209","obj":"T5210"},{"id":"R3345","pred":"parataxis","subj":"T5210","obj":"T5188"}],"text":"To determine if TRIP13 deficiency prevents apoptosis triggered by asynapsis as in C. elegans, we analyzed mice that were doubly mutant for Spo11 and Trip13. SPO11 is a transesterase that is essential for the creation of genetically programmed DSB during leptonema of many organisms, including mice [18]. In C. elegans, spo-11 mutant gametes have extensive asynapsis, which triggers PCH-2 dependent apoptosis in pachynema [2]. In mice, Spo11 −/− spermatocytes are severely defective in homologous chromosome synapsis [34,35], and arrest with chromosomes in a state characteristic of the zygotene/pachytene transition (Figure 3H). Spermatocytes in Trip13Gt/Gt Spo11 −/− testes progressed maximally to that point before undergoing death (Figs 3I), well before the spindle checkpoint that eliminates achiasmate spermatocytes [36]. There was no evidence of metaphase I spermatocytes or postmeiotic spermatids in these testes, unlike those seen in Trip13 single mutants (Figure 3G). In contrast to the complete synapsis in Trip13Gt/Gt pachytene spermatocytes (Figure 5A), in which SPO11 is available in leptonema to initiate (via DSB induction, Figure S2A and S2B) a recombination-driven homolog search, chromosome synapsis in doubly mutant spermatocytes was highly disrupted as in Spo11 single mutants (Figure 5B and 5C). Identical studies were performed with mice deficient for Mei1, a vertebrate-specific gene also required for DSB formation and chromosome synapsis [37], with similar results (Figure 3J and 3K; immunocytology not shown)."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T4581","span":{"begin":16,"end":22},"obj":"PR:000016672"},{"id":"T4582","span":{"begin":43,"end":52},"obj":"GO:0006915"},{"id":"T4583","span":{"begin":67,"end":75},"obj":"GO:0007129"},{"id":"T4584","span":{"begin":82,"end":92},"obj":"NCBITaxon:6239"},{"id":"T4585","span":{"begin":106,"end":110},"obj":"NCBITaxon:10088"},{"id":"T4586","span":{"begin":139,"end":144},"obj":"PR:000015553"},{"id":"T4587","span":{"begin":149,"end":155},"obj":"PR:000016672"},{"id":"T4588","span":{"begin":157,"end":162},"obj":"PR:000015553"},{"id":"T4589","span":{"begin":220,"end":231},"obj":"SO:0000704"},{"id":"T4590","span":{"begin":254,"end":263},"obj":"GO:0000237"},{"id":"T4591","span":{"begin":272,"end":281},"obj":"NCBITaxon:1"},{"id":"T4592","span":{"begin":293,"end":297},"obj":"NCBITaxon:10088"},{"id":"T4593","span":{"begin":307,"end":317},"obj":"NCBITaxon:6239"},{"id":"T4594","span":{"begin":319,"end":325},"obj":"PR:Q22236"},{"id":"T4595","span":{"begin":333,"end":340},"obj":"CL:0000300"},{"id":"T4596","span":{"begin":357,"end":365},"obj":"GO:0007129"},{"id":"T4597","span":{"begin":382,"end":387},"obj":"PR:P38126"},{"id":"T4598","span":{"begin":398,"end":407},"obj":"GO:0006915"},{"id":"T4599","span":{"begin":411,"end":420},"obj":"GO:0000239"},{"id":"T4600","span":{"begin":429,"end":433},"obj":"NCBITaxon:10088"},{"id":"T4601","span":{"begin":435,"end":440},"obj":"PR:000015553"},{"id":"T4602","span":{"begin":445,"end":458},"obj":"CL:0000017"},{"id":"T4603","span":{"begin":485,"end":515},"obj":"GO:0007129"},{"id":"T4604","span":{"begin":586,"end":594},"obj":"GO:0000238"},{"id":"T4605","span":{"begin":595,"end":604},"obj":"GO:0000239"},{"id":"T4606","span":{"begin":629,"end":642},"obj":"CL:0000017"},{"id":"T4607","span":{"begin":646,"end":652},"obj":"PR:000016672"},{"id":"T4608","span":{"begin":658,"end":663},"obj":"PR:000015553"},{"id":"T4609","span":{"begin":668,"end":674},"obj":"UBERON:0000473"},{"id":"T4610","span":{"begin":728,"end":733},"obj":"GO:0016265"},{"id":"T4611","span":{"begin":761,"end":768},"obj":"GO:0005819"},{"id":"T4612","span":{"begin":761,"end":779},"obj":"GO:0031577"},{"id":"T4613","span":{"begin":797,"end":806},"obj":"GO:0005712"},{"id":"T4614","span":{"begin":807,"end":820},"obj":"CL:0000017"},{"id":"T4615","span":{"begin":852,"end":863},"obj":"GO:0007132"},{"id":"T4616","span":{"begin":864,"end":877},"obj":"CL:0000017"},{"id":"T4617","span":{"begin":885,"end":892},"obj":"GO:0007126"},{"id":"T4618","span":{"begin":893,"end":903},"obj":"CL:0000018"},{"id":"T4619","span":{"begin":913,"end":919},"obj":"UBERON:0000473"},{"id":"T4620","span":{"begin":942,"end":948},"obj":"PR:000016672"},{"id":"T4621","span":{"begin":1005,"end":1013},"obj":"GO:0007129"},{"id":"T4622","span":{"begin":1017,"end":1023},"obj":"PR:000016672"},{"id":"T4623","span":{"begin":1029,"end":1038},"obj":"GO:0000239"},{"id":"T4624","span":{"begin":1039,"end":1052},"obj":"CL:0000017"},{"id":"T4625","span":{"begin":1075,"end":1080},"obj":"PR:000015553"},{"id":"T4626","span":{"begin":1097,"end":1106},"obj":"GO:0000237"},{"id":"T4627","span":{"begin":1198,"end":1217},"obj":"GO:0007129"},{"id":"T4628","span":{"begin":1235,"end":1248},"obj":"CL:0000017"},{"id":"T4629","span":{"begin":1276,"end":1281},"obj":"PR:000015553"},{"id":"T4630","span":{"begin":1355,"end":1359},"obj":"NCBITaxon:10088"},{"id":"T4631","span":{"begin":1374,"end":1378},"obj":"PR:000031227"},{"id":"T4632","span":{"begin":1382,"end":1392},"obj":"NCBITaxon:7742"},{"id":"T4633","span":{"begin":1402,"end":1406},"obj":"SO:0000704"},{"id":"T4634","span":{"begin":1443,"end":1462},"obj":"GO:0007129"}],"text":"To determine if TRIP13 deficiency prevents apoptosis triggered by asynapsis as in C. elegans, we analyzed mice that were doubly mutant for Spo11 and Trip13. SPO11 is a transesterase that is essential for the creation of genetically programmed DSB during leptonema of many organisms, including mice [18]. In C. elegans, spo-11 mutant gametes have extensive asynapsis, which triggers PCH-2 dependent apoptosis in pachynema [2]. In mice, Spo11 −/− spermatocytes are severely defective in homologous chromosome synapsis [34,35], and arrest with chromosomes in a state characteristic of the zygotene/pachytene transition (Figure 3H). Spermatocytes in Trip13Gt/Gt Spo11 −/− testes progressed maximally to that point before undergoing death (Figs 3I), well before the spindle checkpoint that eliminates achiasmate spermatocytes [36]. There was no evidence of metaphase I spermatocytes or postmeiotic spermatids in these testes, unlike those seen in Trip13 single mutants (Figure 3G). In contrast to the complete synapsis in Trip13Gt/Gt pachytene spermatocytes (Figure 5A), in which SPO11 is available in leptonema to initiate (via DSB induction, Figure S2A and S2B) a recombination-driven homolog search, chromosome synapsis in doubly mutant spermatocytes was highly disrupted as in Spo11 single mutants (Figure 5B and 5C). Identical studies were performed with mice deficient for Mei1, a vertebrate-specific gene also required for DSB formation and chromosome synapsis [37], with similar results (Figure 3J and 3K; immunocytology not shown)."}

{"project":"2_test","denotations":[{"id":"17696610-11242108-85497914","span":{"begin":299,"end":301},"obj":"11242108"},{"id":"17696610-16339446-85497915","span":{"begin":422,"end":423},"obj":"16339446"},{"id":"17696610-11106739-85497916","span":{"begin":517,"end":519},"obj":"11106739"},{"id":"17696610-11106738-85497917","span":{"begin":520,"end":522},"obj":"11106738"},{"id":"17696610-11686299-85497918","span":{"begin":822,"end":824},"obj":"11686299"},{"id":"17696610-14668445-85497919","span":{"begin":1464,"end":1466},"obj":"14668445"},{"id":"T61286","span":{"begin":299,"end":301},"obj":"11242108"},{"id":"T89712","span":{"begin":422,"end":423},"obj":"16339446"},{"id":"T45258","span":{"begin":517,"end":519},"obj":"11106739"},{"id":"T41168","span":{"begin":520,"end":522},"obj":"11106738"},{"id":"T50334","span":{"begin":822,"end":824},"obj":"11686299"},{"id":"T60577","span":{"begin":1464,"end":1466},"obj":"14668445"}],"text":"To determine if TRIP13 deficiency prevents apoptosis triggered by asynapsis as in C. elegans, we analyzed mice that were doubly mutant for Spo11 and Trip13. SPO11 is a transesterase that is essential for the creation of genetically programmed DSB during leptonema of many organisms, including mice [18]. In C. elegans, spo-11 mutant gametes have extensive asynapsis, which triggers PCH-2 dependent apoptosis in pachynema [2]. In mice, Spo11 −/− spermatocytes are severely defective in homologous chromosome synapsis [34,35], and arrest with chromosomes in a state characteristic of the zygotene/pachytene transition (Figure 3H). Spermatocytes in Trip13Gt/Gt Spo11 −/− testes progressed maximally to that point before undergoing death (Figs 3I), well before the spindle checkpoint that eliminates achiasmate spermatocytes [36]. There was no evidence of metaphase I spermatocytes or postmeiotic spermatids in these testes, unlike those seen in Trip13 single mutants (Figure 3G). In contrast to the complete synapsis in Trip13Gt/Gt pachytene spermatocytes (Figure 5A), in which SPO11 is available in leptonema to initiate (via DSB induction, Figure S2A and S2B) a recombination-driven homolog search, chromosome synapsis in doubly mutant spermatocytes was highly disrupted as in Spo11 single mutants (Figure 5B and 5C). Identical studies were performed with mice deficient for Mei1, a vertebrate-specific gene also required for DSB formation and chromosome synapsis [37], with similar results (Figure 3J and 3K; immunocytology not shown)."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T4723","span":{"begin":16,"end":22},"obj":"PR_EXT:000016672"},{"id":"T4724","span":{"begin":43,"end":52},"obj":"GO:0006915"},{"id":"T4725","span":{"begin":67,"end":75},"obj":"GO:0007129"},{"id":"T4726","span":{"begin":82,"end":92},"obj":"NCBITaxon:6239"},{"id":"T4727","span":{"begin":106,"end":110},"obj":"NCBITaxon:10088"},{"id":"T4728","span":{"begin":128,"end":134},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T4729","span":{"begin":139,"end":144},"obj":"PR_EXT:000015553"},{"id":"T4730","span":{"begin":149,"end":155},"obj":"PR_EXT:000016672"},{"id":"T4731","span":{"begin":157,"end":162},"obj":"PR_EXT:000015553"},{"id":"T4732","span":{"begin":220,"end":231},"obj":"SO_EXT:0000704"},{"id":"T4733","span":{"begin":254,"end":263},"obj":"GO:0000237"},{"id":"T4734","span":{"begin":272,"end":281},"obj":"NCBITaxon:1"},{"id":"T4735","span":{"begin":293,"end":297},"obj":"NCBITaxon:10088"},{"id":"T4736","span":{"begin":307,"end":317},"obj":"NCBITaxon:6239"},{"id":"T4737","span":{"begin":319,"end":325},"obj":"PR_EXT:Q22236"},{"id":"T4738","span":{"begin":326,"end":332},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T4739","span":{"begin":333,"end":340},"obj":"CL:0000300"},{"id":"T4740","span":{"begin":357,"end":365},"obj":"GO:0007129"},{"id":"T4741","span":{"begin":382,"end":387},"obj":"PR_EXT:P38126"},{"id":"T4742","span":{"begin":398,"end":407},"obj":"GO:0006915"},{"id":"T4743","span":{"begin":411,"end":420},"obj":"GO:0000239"},{"id":"T4744","span":{"begin":429,"end":433},"obj":"NCBITaxon:10088"},{"id":"T4745","span":{"begin":435,"end":440},"obj":"PR_EXT:000015553"},{"id":"T4746","span":{"begin":441,"end":442},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4747","span":{"begin":443,"end":444},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4748","span":{"begin":445,"end":458},"obj":"CL:0000017"},{"id":"T4749","span":{"begin":485,"end":515},"obj":"GO:0007129"},{"id":"T4750","span":{"begin":496,"end":506},"obj":"GO_SO_EXT:chromosome"},{"id":"T4751","span":{"begin":541,"end":552},"obj":"GO_SO_EXT:chromosome"},{"id":"T4752","span":{"begin":586,"end":594},"obj":"GO:0000238"},{"id":"T4753","span":{"begin":595,"end":604},"obj":"GO:0000239"},{"id":"T4754","span":{"begin":629,"end":642},"obj":"CL:0000017"},{"id":"T4755","span":{"begin":646,"end":652},"obj":"PR_EXT:000016672"},{"id":"T4756","span":{"begin":658,"end":663},"obj":"PR_EXT:000015553"},{"id":"T4757","span":{"begin":664,"end":665},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4758","span":{"begin":666,"end":667},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4759","span":{"begin":668,"end":674},"obj":"UBERON:0000473"},{"id":"T4760","span":{"begin":728,"end":733},"obj":"GO:0016265"},{"id":"T4761","span":{"begin":761,"end":768},"obj":"GO:0005819"},{"id":"T4762","span":{"begin":761,"end":779},"obj":"GO:0031577"},{"id":"T4763","span":{"begin":785,"end":795},"obj":"GO_EXT:killing"},{"id":"T4764","span":{"begin":797,"end":806},"obj":"GO:0005712"},{"id":"T4765","span":{"begin":807,"end":820},"obj":"CL:0000017"},{"id":"T4766","span":{"begin":852,"end":863},"obj":"GO:0007132"},{"id":"T4767","span":{"begin":864,"end":877},"obj":"CL:0000017"},{"id":"T4768","span":{"begin":885,"end":892},"obj":"GO:0007126"},{"id":"T4769","span":{"begin":893,"end":903},"obj":"CL:0000018"},{"id":"T4770","span":{"begin":913,"end":919},"obj":"UBERON:0000473"},{"id":"T4771","span":{"begin":942,"end":948},"obj":"PR_EXT:000016672"},{"id":"T4772","span":{"begin":956,"end":963},"obj":"SO_EXT:sequence_altered_entity"},{"id":"T4773","span":{"begin":1005,"end":1013},"obj":"GO:0007129"},{"id":"T4774","span":{"begin":1017,"end":1023},"obj":"PR_EXT:000016672"},{"id":"T4775","span":{"begin":1029,"end":1038},"obj":"GO:0000239"},{"id":"T4776","span":{"begin":1039,"end":1052},"obj":"CL:0000017"},{"id":"T4777","span":{"begin":1075,"end":1080},"obj":"PR_EXT:000015553"},{"id":"T4778","span":{"begin":1097,"end":1106},"obj":"GO:0000237"},{"id":"T4779","span":{"begin":1161,"end":1174},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T4780","span":{"begin":1198,"end":1208},"obj":"GO_SO_EXT:chromosome"},{"id":"T4781","span":{"begin":1198,"end":1217},"obj":"GO:0007129"},{"id":"T4782","span":{"begin":1228,"end":1234},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T4783","span":{"begin":1235,"end":1248},"obj":"CL:0000017"},{"id":"T4786","span":{"begin":1355,"end":1359},"obj":"NCBITaxon:10088"},{"id":"T4784","span":{"begin":1276,"end":1281},"obj":"PR_EXT:000015553"},{"id":"T4785","span":{"begin":1289,"end":1296},"obj":"SO_EXT:sequence_altered_entity"},{"id":"T4787","span":{"begin":1374,"end":1378},"obj":"PR_EXT:000031227"},{"id":"T4788","span":{"begin":1382,"end":1392},"obj":"NCBITaxon:7742"},{"id":"T4789","span":{"begin":1402,"end":1406},"obj":"SO_EXT:0000704"},{"id":"T4790","span":{"begin":1443,"end":1453},"obj":"GO_SO_EXT:chromosome"},{"id":"T4791","span":{"begin":1443,"end":1462},"obj":"GO:0007129"}],"text":"To determine if TRIP13 deficiency prevents apoptosis triggered by asynapsis as in C. elegans, we analyzed mice that were doubly mutant for Spo11 and Trip13. SPO11 is a transesterase that is essential for the creation of genetically programmed DSB during leptonema of many organisms, including mice [18]. In C. elegans, spo-11 mutant gametes have extensive asynapsis, which triggers PCH-2 dependent apoptosis in pachynema [2]. In mice, Spo11 −/− spermatocytes are severely defective in homologous chromosome synapsis [34,35], and arrest with chromosomes in a state characteristic of the zygotene/pachytene transition (Figure 3H). Spermatocytes in Trip13Gt/Gt Spo11 −/− testes progressed maximally to that point before undergoing death (Figs 3I), well before the spindle checkpoint that eliminates achiasmate spermatocytes [36]. There was no evidence of metaphase I spermatocytes or postmeiotic spermatids in these testes, unlike those seen in Trip13 single mutants (Figure 3G). In contrast to the complete synapsis in Trip13Gt/Gt pachytene spermatocytes (Figure 5A), in which SPO11 is available in leptonema to initiate (via DSB induction, Figure S2A and S2B) a recombination-driven homolog search, chromosome synapsis in doubly mutant spermatocytes was highly disrupted as in Spo11 single mutants (Figure 5B and 5C). Identical studies were performed with mice deficient for Mei1, a vertebrate-specific gene also required for DSB formation and chromosome synapsis [37], with similar results (Figure 3J and 3K; immunocytology not shown)."}