Id |
Subject |
Object |
Predicate |
Lexical cue |
T6759 |
0-10 |
NN |
denotes |
Expression |
T6760 |
11-13 |
IN |
denotes |
of |
T6761 |
14-19 |
NN |
denotes |
DMRT7 |
T6762 |
20-28 |
NNS |
denotes |
Proteins |
T6763 |
29-31 |
IN |
denotes |
in |
T6764 |
32-38 |
NN |
denotes |
Testis |
T6765 |
38-206 |
sentence |
denotes |
Our previous mRNA expression analysis suggested a possible meiotic function for Dmrt7, based on the expression of Dmrt7 mRNA in the fetal gonads of the two sexes [23]. |
T6766 |
39-42 |
PRP$ |
denotes |
Our |
T6767 |
68-76 |
NN |
denotes |
analysis |
T6768 |
43-51 |
JJ |
denotes |
previous |
T6769 |
52-56 |
NN |
denotes |
mRNA |
T6770 |
57-67 |
NN |
denotes |
expression |
T6771 |
77-86 |
VBD |
denotes |
suggested |
T6772 |
87-88 |
DT |
denotes |
a |
T6773 |
106-114 |
NN |
denotes |
function |
T6774 |
89-97 |
JJ |
denotes |
possible |
T6775 |
98-105 |
JJ |
denotes |
meiotic |
T6776 |
115-118 |
IN |
denotes |
for |
T6777 |
119-124 |
NN |
denotes |
Dmrt7 |
T6778 |
124-126 |
, |
denotes |
, |
T6779 |
126-131 |
VBN |
denotes |
based |
T6780 |
132-134 |
IN |
denotes |
on |
T6781 |
135-138 |
DT |
denotes |
the |
T6782 |
139-149 |
NN |
denotes |
expression |
T6783 |
150-152 |
IN |
denotes |
of |
T6784 |
153-158 |
NN |
denotes |
Dmrt7 |
T6785 |
159-163 |
NN |
denotes |
mRNA |
T6786 |
164-166 |
IN |
denotes |
in |
T6787 |
167-170 |
DT |
denotes |
the |
T6788 |
177-183 |
NNS |
denotes |
gonads |
T6789 |
171-176 |
JJ |
denotes |
fetal |
T6790 |
184-186 |
IN |
denotes |
of |
T6791 |
187-190 |
DT |
denotes |
the |
T6792 |
195-200 |
NNS |
denotes |
sexes |
T6793 |
191-194 |
CD |
denotes |
two |
T6794 |
201-202 |
-LRB- |
denotes |
[ |
T6795 |
202-204 |
CD |
denotes |
23 |
T6796 |
204-205 |
-RRB- |
denotes |
] |
T6797 |
205-206 |
. |
denotes |
. |
T6798 |
206-450 |
sentence |
denotes |
In the fetal ovary, Dmrt7 mRNA was detected primarily from E13.5 to E15.5, the time during which meiosis progresses from pre-meiotic replication to the pachytene stage [4], whereas Dmrt7 expression in the non-meiotic fetal testis was very low. |
T6799 |
207-209 |
IN |
denotes |
In |
T6800 |
242-250 |
VBN |
denotes |
detected |
T6801 |
210-213 |
DT |
denotes |
the |
T6802 |
220-225 |
NN |
denotes |
ovary |
T6803 |
214-219 |
JJ |
denotes |
fetal |
T6804 |
225-227 |
, |
denotes |
, |
T6805 |
227-232 |
NN |
denotes |
Dmrt7 |
T6806 |
233-237 |
NN |
denotes |
mRNA |
T6807 |
238-241 |
VBD |
denotes |
was |
T6808 |
251-260 |
RB |
denotes |
primarily |
T6809 |
261-265 |
IN |
denotes |
from |
T6810 |
266-271 |
NN |
denotes |
E13.5 |
T6811 |
272-274 |
IN |
denotes |
to |
T6812 |
275-280 |
NN |
denotes |
E15.5 |
T6813 |
280-282 |
, |
denotes |
, |
T6814 |
282-285 |
DT |
denotes |
the |
T6815 |
286-290 |
NN |
denotes |
time |
T6816 |
291-297 |
IN |
denotes |
during |
T6817 |
312-322 |
VBZ |
denotes |
progresses |
T6818 |
298-303 |
WDT |
denotes |
which |
T6819 |
304-311 |
NN |
denotes |
meiosis |
T6820 |
323-327 |
IN |
denotes |
from |
T6821 |
328-339 |
JJ |
denotes |
pre-meiotic |
T6822 |
340-351 |
NN |
denotes |
replication |
T6823 |
352-354 |
IN |
denotes |
to |
T6824 |
355-358 |
DT |
denotes |
the |
T6825 |
369-374 |
NN |
denotes |
stage |
T6826 |
359-368 |
NN |
denotes |
pachytene |
T6827 |
375-376 |
-LRB- |
denotes |
[ |
T6828 |
376-377 |
CD |
denotes |
4 |
T6829 |
377-378 |
-RRB- |
denotes |
] |
T6830 |
378-380 |
, |
denotes |
, |
T6831 |
380-387 |
IN |
denotes |
whereas |
T6832 |
437-440 |
VBD |
denotes |
was |
T6833 |
388-393 |
NN |
denotes |
Dmrt7 |
T6834 |
394-404 |
NN |
denotes |
expression |
T6835 |
405-407 |
IN |
denotes |
in |
T6836 |
408-411 |
DT |
denotes |
the |
T6837 |
430-436 |
NN |
denotes |
testis |
T6838 |
412-423 |
JJ |
denotes |
non-meiotic |
T6839 |
424-429 |
JJ |
denotes |
fetal |
T6840 |
441-445 |
RB |
denotes |
very |
T6841 |
446-449 |
JJ |
denotes |
low |
T6842 |
449-450 |
. |
denotes |
. |
T6843 |
450-736 |
sentence |
denotes |
Because this earlier work did not examine adult Dmrt7 expression, we first performed reverse transcriptase (RT)-PCR on mRNA from ten adult organs and detected strong Dmrt7 mRNA expression in the testis and a trace of expression in heart, but not in any other tissue tested (Figure 1A). |
T6844 |
451-458 |
IN |
denotes |
Because |
T6845 |
485-492 |
VB |
denotes |
examine |
T6846 |
459-463 |
DT |
denotes |
this |
T6847 |
472-476 |
NN |
denotes |
work |
T6848 |
464-471 |
JJR |
denotes |
earlier |
T6849 |
477-480 |
VBD |
denotes |
did |
T6850 |
481-484 |
RB |
denotes |
not |
T6851 |
526-535 |
VBD |
denotes |
performed |
T6852 |
493-498 |
JJ |
denotes |
adult |
T6853 |
505-515 |
NN |
denotes |
expression |
T6856 |
517-519 |
PRP |
denotes |
we |
T6857 |
520-525 |
RB |
denotes |
first |
T6858 |
536-543 |
JJ |
denotes |
reverse |
T6859 |
544-557 |
NN |
denotes |
transcriptase |
T6860 |
563-566 |
NN |
denotes |
PCR |
T6861 |
558-559 |
-LRB- |
denotes |
( |
T6862 |
559-561 |
NN |
denotes |
RT |
T6863 |
561-562 |
-RRB- |
denotes |
) |
T6864 |
562-563 |
HYPH |
denotes |
- |
T6865 |
567-569 |
IN |
denotes |
on |
T6866 |
570-574 |
NN |
denotes |
mRNA |
T6867 |
575-579 |
IN |
denotes |
from |
T6868 |
580-583 |
CD |
denotes |
ten |
T6869 |
590-596 |
NNS |
denotes |
organs |
T6870 |
584-589 |
JJ |
denotes |
adult |
T6871 |
597-600 |
CC |
denotes |
and |
T6872 |
601-609 |
VBN |
denotes |
detected |
T6873 |
610-616 |
JJ |
denotes |
strong |
T6874 |
628-638 |
NN |
denotes |
expression |
T6875 |
617-622 |
NN |
denotes |
Dmrt7 |
T6876 |
623-627 |
NN |
denotes |
mRNA |
T6877 |
639-641 |
IN |
denotes |
in |
T6878 |
642-645 |
DT |
denotes |
the |
T6879 |
646-652 |
NN |
denotes |
testis |
T6880 |
653-656 |
CC |
denotes |
and |
T6881 |
657-658 |
DT |
denotes |
a |
T6882 |
659-664 |
NN |
denotes |
trace |
T6883 |
665-667 |
IN |
denotes |
of |
T6884 |
668-678 |
NN |
denotes |
expression |
T6885 |
679-681 |
IN |
denotes |
in |
T6886 |
682-687 |
NN |
denotes |
heart |
T6887 |
687-689 |
, |
denotes |
, |
T6888 |
689-692 |
CC |
denotes |
but |
T6889 |
693-696 |
RB |
denotes |
not |
T6890 |
697-699 |
IN |
denotes |
in |
T6891 |
700-703 |
DT |
denotes |
any |
T6892 |
710-716 |
NN |
denotes |
tissue |
T6893 |
704-709 |
JJ |
denotes |
other |
T6894 |
717-723 |
VBN |
denotes |
tested |
T6895 |
724-725 |
-LRB- |
denotes |
( |
T6896 |
732-734 |
NN |
denotes |
1A |
T6897 |
725-731 |
NN |
denotes |
Figure |
T6898 |
734-735 |
-RRB- |
denotes |
) |
T6899 |
735-736 |
. |
denotes |
. |
T6900 |
736-1003 |
sentence |
denotes |
We examined the timing of Dmrt7 mRNA expression during postnatal testis development and detected strong expression beginning at 2 wk, which roughly coincides with the onset of the pachytene stage during the first synchronous wave of spermatogenesis (Figure 1B) [37]. |
T6901 |
737-739 |
PRP |
denotes |
We |
T6902 |
740-748 |
VBD |
denotes |
examined |
T6903 |
749-752 |
DT |
denotes |
the |
T6904 |
753-759 |
NN |
denotes |
timing |
T6905 |
760-762 |
IN |
denotes |
of |
T6906 |
763-768 |
NN |
denotes |
Dmrt7 |
T6907 |
774-784 |
NN |
denotes |
expression |
T6908 |
769-773 |
NN |
denotes |
mRNA |
T6909 |
785-791 |
IN |
denotes |
during |
T6910 |
792-801 |
JJ |
denotes |
postnatal |
T6911 |
809-820 |
NN |
denotes |
development |
T6912 |
802-808 |
NN |
denotes |
testis |
T6913 |
821-824 |
CC |
denotes |
and |
T6914 |
825-833 |
VBD |
denotes |
detected |
T6915 |
834-840 |
JJ |
denotes |
strong |
T6916 |
841-851 |
NN |
denotes |
expression |
T6917 |
852-861 |
VBG |
denotes |
beginning |
T6918 |
862-864 |
IN |
denotes |
at |
T6919 |
865-866 |
CD |
denotes |
2 |
T6920 |
867-869 |
NNS |
denotes |
wk |
T6921 |
869-871 |
, |
denotes |
, |
T6922 |
871-876 |
WDT |
denotes |
which |
T6923 |
885-894 |
VBZ |
denotes |
coincides |
T6924 |
877-884 |
RB |
denotes |
roughly |
T6925 |
895-899 |
IN |
denotes |
with |
T6926 |
900-903 |
DT |
denotes |
the |
T6927 |
904-909 |
NN |
denotes |
onset |
T6928 |
910-912 |
IN |
denotes |
of |
T6929 |
913-916 |
DT |
denotes |
the |
T6930 |
927-932 |
NN |
denotes |
stage |
T6931 |
917-926 |
NN |
denotes |
pachytene |
T6932 |
933-939 |
IN |
denotes |
during |
T6933 |
940-943 |
DT |
denotes |
the |
T6934 |
962-966 |
NN |
denotes |
wave |
T6935 |
944-949 |
JJ |
denotes |
first |
T6936 |
950-961 |
JJ |
denotes |
synchronous |
T6937 |
967-969 |
IN |
denotes |
of |
T6938 |
970-985 |
NN |
denotes |
spermatogenesis |
T6939 |
986-987 |
-LRB- |
denotes |
( |
T6940 |
994-996 |
NN |
denotes |
1B |
T6941 |
987-993 |
NN |
denotes |
Figure |
T6942 |
996-997 |
-RRB- |
denotes |
) |
T6943 |
998-999 |
-LRB- |
denotes |
[ |
T6944 |
999-1001 |
CD |
denotes |
37 |
T6945 |
1001-1002 |
-RRB- |
denotes |
] |
T6946 |
1002-1003 |
. |
denotes |
. |
T6947 |
1003-1004 |
sentence |
denotes |
|
T6948 |
1823-1935 |
sentence |
denotes |
To investigate DMRT7 protein expression, we generated an antibody against the C-terminal portion of the protein. |
T6949 |
1823-1825 |
TO |
denotes |
To |
T6950 |
1826-1837 |
VB |
denotes |
investigate |
T6951 |
1867-1876 |
VBD |
denotes |
generated |
T6952 |
1838-1843 |
NN |
denotes |
DMRT7 |
T6953 |
1852-1862 |
NN |
denotes |
expression |
T6954 |
1844-1851 |
NN |
denotes |
protein |
T6955 |
1862-1864 |
, |
denotes |
, |
T6956 |
1864-1866 |
PRP |
denotes |
we |
T6957 |
1877-1879 |
DT |
denotes |
an |
T6958 |
1880-1888 |
NN |
denotes |
antibody |
T6959 |
1889-1896 |
IN |
denotes |
against |
T6960 |
1897-1900 |
DT |
denotes |
the |
T6961 |
1912-1919 |
NN |
denotes |
portion |
T6962 |
1901-1902 |
NN |
denotes |
C |
T6963 |
1903-1911 |
JJ |
denotes |
terminal |
T6964 |
1902-1903 |
HYPH |
denotes |
- |
T6965 |
1920-1922 |
IN |
denotes |
of |
T6966 |
1923-1926 |
DT |
denotes |
the |
T6967 |
1927-1934 |
NN |
denotes |
protein |
T6968 |
1934-1935 |
. |
denotes |
. |
T6969 |
1935-2069 |
sentence |
denotes |
The antibody was raised against a unique region lacking the DM domain in order to avoid cross-reaction with other DM domain proteins. |
T6970 |
1936-1939 |
DT |
denotes |
The |
T6971 |
1940-1948 |
NN |
denotes |
antibody |
T6972 |
1953-1959 |
VBN |
denotes |
raised |
T6973 |
1949-1952 |
VBD |
denotes |
was |
T6974 |
1960-1967 |
IN |
denotes |
against |
T6975 |
1968-1969 |
DT |
denotes |
a |
T6976 |
1977-1983 |
NN |
denotes |
region |
T6977 |
1970-1976 |
JJ |
denotes |
unique |
T6978 |
1984-1991 |
VBG |
denotes |
lacking |
T6979 |
1992-1995 |
DT |
denotes |
the |
T6980 |
1999-2005 |
NN |
denotes |
domain |
T6981 |
1996-1998 |
NN |
denotes |
DM |
T6982 |
2006-2008 |
IN |
denotes |
in |
T6983 |
2009-2014 |
NN |
denotes |
order |
T6984 |
2015-2017 |
TO |
denotes |
to |
T6985 |
2018-2023 |
VB |
denotes |
avoid |
T6986 |
2024-2038 |
NN |
denotes |
cross-reaction |
T6987 |
2039-2043 |
IN |
denotes |
with |
T6988 |
2044-2049 |
JJ |
denotes |
other |
T6989 |
2060-2068 |
NN |
denotes |
proteins |
T6990 |
2050-2052 |
NN |
denotes |
DM |
T6991 |
2053-2059 |
NN |
denotes |
domain |
T6992 |
2068-2069 |
. |
denotes |
. |
T6993 |
2069-2345 |
sentence |
denotes |
Immunofluorescent staining with purified DMRT7 antisera showed that DMRT7 protein is expressed predominantly in mid- to late-pachytene spermatocytes (Figure 1C), as well as in sperm, and is not detectable in other germ cell types including spermatogonia and round spermatids. |
T6994 |
2070-2087 |
JJ |
denotes |
Immunofluorescent |
T6995 |
2088-2096 |
NN |
denotes |
staining |
T6996 |
2126-2132 |
VBD |
denotes |
showed |
T6997 |
2097-2101 |
IN |
denotes |
with |
T6998 |
2102-2110 |
VBN |
denotes |
purified |
T6999 |
2117-2125 |
NNS |
denotes |
antisera |
T7000 |
2111-2116 |
NN |
denotes |
DMRT7 |
T7001 |
2133-2137 |
IN |
denotes |
that |
T7002 |
2155-2164 |
VBN |
denotes |
expressed |
T7003 |
2138-2143 |
NN |
denotes |
DMRT7 |
T7004 |
2144-2151 |
NN |
denotes |
protein |
T7005 |
2152-2154 |
VBZ |
denotes |
is |
T7006 |
2165-2178 |
RB |
denotes |
predominantly |
T7007 |
2179-2181 |
IN |
denotes |
in |
T7008 |
2182-2185 |
JJ |
denotes |
mid |
T7009 |
2195-2204 |
NN |
denotes |
pachytene |
T7010 |
2185-2186 |
HYPH |
denotes |
- |
T7011 |
2187-2189 |
IN |
denotes |
to |
T7012 |
2190-2194 |
JJ |
denotes |
late |
T7013 |
2194-2195 |
HYPH |
denotes |
- |
T7014 |
2205-2218 |
NNS |
denotes |
spermatocytes |
T7015 |
2219-2220 |
-LRB- |
denotes |
( |
T7016 |
2227-2229 |
NN |
denotes |
1C |
T7017 |
2220-2226 |
NN |
denotes |
Figure |
T7018 |
2229-2230 |
-RRB- |
denotes |
) |
T7019 |
2230-2232 |
, |
denotes |
, |
T7020 |
2232-2234 |
RB |
denotes |
as |
T7021 |
2240-2242 |
IN |
denotes |
as |
T7022 |
2235-2239 |
RB |
denotes |
well |
T7023 |
2243-2245 |
IN |
denotes |
in |
T7024 |
2246-2251 |
NN |
denotes |
sperm |
T7025 |
2251-2253 |
, |
denotes |
, |
T7026 |
2253-2256 |
CC |
denotes |
and |
T7027 |
2257-2259 |
VBZ |
denotes |
is |
T7028 |
2260-2263 |
RB |
denotes |
not |
T7029 |
2264-2274 |
JJ |
denotes |
detectable |
T7030 |
2275-2277 |
IN |
denotes |
in |
T7031 |
2278-2283 |
JJ |
denotes |
other |
T7032 |
2294-2299 |
NNS |
denotes |
types |
T7033 |
2284-2288 |
NN |
denotes |
germ |
T7034 |
2289-2293 |
NN |
denotes |
cell |
T7035 |
2300-2309 |
VBG |
denotes |
including |
T7036 |
2310-2323 |
NNS |
denotes |
spermatogonia |
T7037 |
2324-2327 |
CC |
denotes |
and |
T7038 |
2328-2333 |
JJ |
denotes |
round |
T7039 |
2334-2344 |
NNS |
denotes |
spermatids |
T7040 |
2344-2345 |
. |
denotes |
. |
T7041 |
2345-2459 |
sentence |
denotes |
We did not detect DMRT7 protein in somatic cells such as Sertoli cells, peritubular myoid cells, or Leydig cells. |
T7042 |
2346-2348 |
PRP |
denotes |
We |
T7043 |
2357-2363 |
VB |
denotes |
detect |
T7044 |
2349-2352 |
VBD |
denotes |
did |
T7045 |
2353-2356 |
RB |
denotes |
not |
T7046 |
2364-2369 |
NN |
denotes |
DMRT7 |
T7047 |
2370-2377 |
NN |
denotes |
protein |
T7048 |
2378-2380 |
IN |
denotes |
in |
T7049 |
2381-2388 |
JJ |
denotes |
somatic |
T7050 |
2389-2394 |
NNS |
denotes |
cells |
T7051 |
2395-2399 |
JJ |
denotes |
such |
T7052 |
2400-2402 |
IN |
denotes |
as |
T7053 |
2403-2410 |
NNP |
denotes |
Sertoli |
T7054 |
2411-2416 |
NNS |
denotes |
cells |
T7055 |
2416-2418 |
, |
denotes |
, |
T7056 |
2418-2429 |
JJ |
denotes |
peritubular |
T7057 |
2436-2441 |
NNS |
denotes |
cells |
T7058 |
2430-2435 |
JJ |
denotes |
myoid |
T7059 |
2441-2443 |
, |
denotes |
, |
T7060 |
2443-2445 |
CC |
denotes |
or |
T7061 |
2446-2452 |
NNP |
denotes |
Leydig |
T7062 |
2453-2458 |
NNS |
denotes |
cells |
T7063 |
2458-2459 |
. |
denotes |
. |
T7064 |
2459-2638 |
sentence |
denotes |
To more precisely determine the pachytene stages of DMRT7 expression, we double-stained with an antibody to GATA1, which is expressed in Sertoli cells from stages VII to IX [38]. |
T7065 |
2460-2462 |
TO |
denotes |
To |
T7066 |
2478-2487 |
VB |
denotes |
determine |
T7067 |
2463-2467 |
RBR |
denotes |
more |
T7068 |
2468-2477 |
RB |
denotes |
precisely |
T7069 |
2540-2547 |
VBN |
denotes |
stained |
T7070 |
2488-2491 |
DT |
denotes |
the |
T7071 |
2502-2508 |
NNS |
denotes |
stages |
T7072 |
2492-2501 |
NN |
denotes |
pachytene |
T7073 |
2509-2511 |
IN |
denotes |
of |
T7074 |
2512-2517 |
NN |
denotes |
DMRT7 |
T7075 |
2518-2528 |
NN |
denotes |
expression |
T7076 |
2528-2530 |
, |
denotes |
, |
T7077 |
2530-2532 |
PRP |
denotes |
we |
T7078 |
2533-2539 |
RB |
denotes |
double |
T7079 |
2539-2540 |
HYPH |
denotes |
- |
T7080 |
2548-2552 |
IN |
denotes |
with |
T7081 |
2553-2555 |
DT |
denotes |
an |
T7082 |
2556-2564 |
NN |
denotes |
antibody |
T7083 |
2565-2567 |
IN |
denotes |
to |
T7084 |
2568-2573 |
NN |
denotes |
GATA1 |
T7085 |
2573-2575 |
, |
denotes |
, |
T7086 |
2575-2580 |
WDT |
denotes |
which |
T7087 |
2584-2593 |
VBN |
denotes |
expressed |
T7088 |
2581-2583 |
VBZ |
denotes |
is |
T7089 |
2594-2596 |
IN |
denotes |
in |
T7090 |
2597-2604 |
NNP |
denotes |
Sertoli |
T7091 |
2605-2610 |
NNS |
denotes |
cells |
T7092 |
2611-2615 |
IN |
denotes |
from |
T7093 |
2616-2622 |
NNS |
denotes |
stages |
T7094 |
2623-2626 |
CD |
denotes |
VII |
T7095 |
2627-2629 |
IN |
denotes |
to |
T7096 |
2630-2632 |
CD |
denotes |
IX |
T7097 |
2633-2634 |
-LRB- |
denotes |
[ |
T7098 |
2634-2636 |
CD |
denotes |
38 |
T7099 |
2636-2637 |
-RRB- |
denotes |
] |
T7100 |
2637-2638 |
. |
denotes |
. |
T7101 |
2638-2810 |
sentence |
denotes |
This confirmed that DMRT7 is expressed in mid- to late-pachytene spermatocytes, starting slightly earlier than stage VII and extending through stage IX (unpublished data). |
T7102 |
2639-2643 |
DT |
denotes |
This |
T7103 |
2644-2653 |
VBD |
denotes |
confirmed |
T7104 |
2654-2658 |
IN |
denotes |
that |
T7105 |
2668-2677 |
VBN |
denotes |
expressed |
T7106 |
2659-2664 |
NN |
denotes |
DMRT7 |
T7107 |
2665-2667 |
VBZ |
denotes |
is |
T7108 |
2678-2680 |
IN |
denotes |
in |
T7109 |
2681-2684 |
JJ |
denotes |
mid |
T7110 |
2694-2703 |
NN |
denotes |
pachytene |
T7111 |
2684-2685 |
HYPH |
denotes |
- |
T7112 |
2686-2688 |
IN |
denotes |
to |
T7113 |
2689-2693 |
JJ |
denotes |
late |
T7114 |
2693-2694 |
HYPH |
denotes |
- |
T7115 |
2704-2717 |
NNS |
denotes |
spermatocytes |
T7116 |
2717-2719 |
, |
denotes |
, |
T7117 |
2719-2727 |
VBG |
denotes |
starting |
T7118 |
2728-2736 |
RB |
denotes |
slightly |
T7119 |
2737-2744 |
RBR |
denotes |
earlier |
T7120 |
2745-2749 |
IN |
denotes |
than |
T7121 |
2750-2755 |
NN |
denotes |
stage |
T7122 |
2756-2759 |
CD |
denotes |
VII |
T7123 |
2760-2763 |
CC |
denotes |
and |
T7124 |
2764-2773 |
VBG |
denotes |
extending |
T7125 |
2774-2781 |
IN |
denotes |
through |
T7126 |
2782-2787 |
NN |
denotes |
stage |
T7127 |
2788-2790 |
CD |
denotes |
IX |
T7128 |
2791-2792 |
-LRB- |
denotes |
( |
T7129 |
2804-2808 |
NNS |
denotes |
data |
T7130 |
2792-2803 |
JJ |
denotes |
unpublished |
T7131 |
2808-2809 |
-RRB- |
denotes |
) |
T7132 |
2809-2810 |
. |
denotes |
. |
T7133 |
2810-2963 |
sentence |
denotes |
Within pachytene spermatocytes, DMRT7 is concentrated in the XY body, or sex body, a densely staining chromatin domain that harbors the sex chromosomes. |
T7134 |
2811-2817 |
IN |
denotes |
Within |
T7135 |
2852-2864 |
VBN |
denotes |
concentrated |
T7136 |
2818-2827 |
NN |
denotes |
pachytene |
T7137 |
2828-2841 |
NNS |
denotes |
spermatocytes |
T7138 |
2841-2843 |
, |
denotes |
, |
T7139 |
2843-2848 |
NN |
denotes |
DMRT7 |
T7140 |
2849-2851 |
VBZ |
denotes |
is |
T7141 |
2865-2867 |
IN |
denotes |
in |
T7142 |
2868-2871 |
DT |
denotes |
the |
T7143 |
2875-2879 |
NN |
denotes |
body |
T7144 |
2872-2874 |
NN |
denotes |
XY |
T7145 |
2879-2881 |
, |
denotes |
, |
T7146 |
2881-2883 |
CC |
denotes |
or |
T7147 |
2884-2887 |
NN |
denotes |
sex |
T7148 |
2888-2892 |
NN |
denotes |
body |
T7149 |
2892-2894 |
, |
denotes |
, |
T7150 |
2894-2895 |
DT |
denotes |
a |
T7151 |
2923-2929 |
NN |
denotes |
domain |
T7152 |
2896-2903 |
RB |
denotes |
densely |
T7153 |
2904-2912 |
VBG |
denotes |
staining |
T7154 |
2913-2922 |
NN |
denotes |
chromatin |
T7155 |
2930-2934 |
WDT |
denotes |
that |
T7156 |
2935-2942 |
VBZ |
denotes |
harbors |
T7157 |
2943-2946 |
DT |
denotes |
the |
T7158 |
2951-2962 |
NNS |
denotes |
chromosomes |
T7159 |
2947-2950 |
NN |
denotes |
sex |
T7160 |
2962-2963 |
. |
denotes |
. |
T7161 |
2963-3121 |
sentence |
denotes |
These undergo transcriptional inactivation and heterochromatinization as a result of their incomplete pairing during prophase of mammalian male meiosis [17]. |
T7162 |
2964-2969 |
DT |
denotes |
These |
T7163 |
2970-2977 |
VBP |
denotes |
undergo |
T7164 |
2978-2993 |
JJ |
denotes |
transcriptional |
T7165 |
2994-3006 |
NN |
denotes |
inactivation |
T7166 |
3007-3010 |
CC |
denotes |
and |
T7167 |
3011-3033 |
NN |
denotes |
heterochromatinization |
T7168 |
3034-3036 |
IN |
denotes |
as |
T7169 |
3037-3038 |
DT |
denotes |
a |
T7170 |
3039-3045 |
NN |
denotes |
result |
T7171 |
3046-3048 |
IN |
denotes |
of |
T7172 |
3049-3054 |
PRP$ |
denotes |
their |
T7173 |
3066-3073 |
NN |
denotes |
pairing |
T7174 |
3055-3065 |
JJ |
denotes |
incomplete |
T7175 |
3074-3080 |
IN |
denotes |
during |
T7176 |
3081-3089 |
NN |
denotes |
prophase |
T7177 |
3090-3092 |
IN |
denotes |
of |
T7178 |
3093-3102 |
JJ |
denotes |
mammalian |
T7179 |
3108-3115 |
NN |
denotes |
meiosis |
T7180 |
3103-3107 |
JJ |
denotes |
male |
T7181 |
3116-3117 |
-LRB- |
denotes |
[ |
T7182 |
3117-3119 |
CD |
denotes |
17 |
T7183 |
3119-3120 |
-RRB- |
denotes |
] |
T7184 |
3120-3121 |
. |
denotes |
. |
T7185 |
3121-3334 |
sentence |
denotes |
To verify DMRT7 protein expression in the XY body, we double-stained mouse testis sections for DMRT7 and small ubiquitin-related modifier 1 (SUMO-1), which is concentrated in the XY body during pachynema [39,40]. |
T7186 |
3122-3124 |
TO |
denotes |
To |
T7187 |
3125-3131 |
VB |
denotes |
verify |
T7188 |
3183-3190 |
VBD |
denotes |
stained |
T7189 |
3132-3137 |
NN |
denotes |
DMRT7 |
T7190 |
3146-3156 |
NN |
denotes |
expression |
T7191 |
3138-3145 |
NN |
denotes |
protein |
T7192 |
3157-3159 |
IN |
denotes |
in |
T7193 |
3160-3163 |
DT |
denotes |
the |
T7194 |
3167-3171 |
NN |
denotes |
body |
T7195 |
3164-3166 |
NN |
denotes |
XY |
T7196 |
3171-3173 |
, |
denotes |
, |
T7197 |
3173-3175 |
PRP |
denotes |
we |
T7198 |
3176-3182 |
RB |
denotes |
double |
T7199 |
3182-3183 |
HYPH |
denotes |
- |
T7200 |
3191-3196 |
NN |
denotes |
mouse |
T7201 |
3197-3203 |
NN |
denotes |
testis |
T7202 |
3204-3212 |
NNS |
denotes |
sections |
T7203 |
3213-3216 |
IN |
denotes |
for |
T7204 |
3217-3222 |
NN |
denotes |
DMRT7 |
T7205 |
3223-3226 |
CC |
denotes |
and |
T7206 |
3227-3232 |
JJ |
denotes |
small |
T7207 |
3251-3259 |
NN |
denotes |
modifier |
T7208 |
3233-3242 |
NN |
denotes |
ubiquitin |
T7209 |
3243-3250 |
VBN |
denotes |
related |
T7210 |
3242-3243 |
HYPH |
denotes |
- |
T7211 |
3260-3261 |
CD |
denotes |
1 |
T7212 |
3262-3263 |
-LRB- |
denotes |
( |
T7213 |
3263-3267 |
NN |
denotes |
SUMO |
T7214 |
3267-3268 |
HYPH |
denotes |
- |
T7215 |
3268-3269 |
CD |
denotes |
1 |
T7216 |
3269-3270 |
-RRB- |
denotes |
) |
T7217 |
3270-3272 |
, |
denotes |
, |
T7218 |
3272-3277 |
WDT |
denotes |
which |
T7219 |
3281-3293 |
VBN |
denotes |
concentrated |
T7220 |
3278-3280 |
VBZ |
denotes |
is |
T7221 |
3294-3296 |
IN |
denotes |
in |
T7222 |
3297-3300 |
DT |
denotes |
the |
T7223 |
3304-3308 |
NN |
denotes |
body |
T7224 |
3301-3303 |
NN |
denotes |
XY |
T7225 |
3309-3315 |
IN |
denotes |
during |
T7226 |
3316-3325 |
NN |
denotes |
pachynema |
T7227 |
3326-3327 |
-LRB- |
denotes |
[ |
T7228 |
3330-3332 |
CD |
denotes |
40 |
T7229 |
3327-3329 |
CD |
denotes |
39 |
T7230 |
3329-3330 |
, |
denotes |
, |
T7231 |
3332-3333 |
-RRB- |
denotes |
] |
T7232 |
3333-3334 |
. |
denotes |
. |
T7233 |
3334-3455 |
sentence |
denotes |
DMRT7 and SUMO-1 were colocalized, confirming that DMRT7 protein is preferentially localized to the XY body (Figure 1D). |
T7234 |
3335-3340 |
NN |
denotes |
DMRT7 |
T7235 |
3357-3368 |
VBN |
denotes |
colocalized |
T7236 |
3341-3344 |
CC |
denotes |
and |
T7237 |
3345-3349 |
NN |
denotes |
SUMO |
T7238 |
3349-3350 |
HYPH |
denotes |
- |
T7239 |
3350-3351 |
CD |
denotes |
1 |
T7240 |
3352-3356 |
VBD |
denotes |
were |
T7241 |
3368-3370 |
, |
denotes |
, |
T7242 |
3370-3380 |
VBG |
denotes |
confirming |
T7243 |
3381-3385 |
IN |
denotes |
that |
T7244 |
3418-3427 |
VBN |
denotes |
localized |
T7245 |
3386-3391 |
NN |
denotes |
DMRT7 |
T7246 |
3392-3399 |
NN |
denotes |
protein |
T7247 |
3400-3402 |
VBZ |
denotes |
is |
T7248 |
3403-3417 |
RB |
denotes |
preferentially |
T7249 |
3428-3430 |
IN |
denotes |
to |
T7250 |
3431-3434 |
DT |
denotes |
the |
T7251 |
3438-3442 |
NN |
denotes |
body |
T7252 |
3435-3437 |
NN |
denotes |
XY |
T7253 |
3443-3444 |
-LRB- |
denotes |
( |
T7254 |
3451-3453 |
NN |
denotes |
1D |
T7255 |
3444-3450 |
NN |
denotes |
Figure |
T7256 |
3453-3454 |
-RRB- |
denotes |
) |
T7257 |
3454-3455 |
. |
denotes |
. |
T7258 |
3455-3587 |
sentence |
denotes |
We also confirmed XY body localization of DMRT7 by double staining for other markers including Ub-H2A and γH2AX (unpublished data). |
T7259 |
3456-3458 |
PRP |
denotes |
We |
T7260 |
3464-3473 |
VBD |
denotes |
confirmed |
T7261 |
3459-3463 |
RB |
denotes |
also |
T7262 |
3474-3476 |
NN |
denotes |
XY |
T7263 |
3482-3494 |
NN |
denotes |
localization |
T7264 |
3477-3481 |
NN |
denotes |
body |
T7265 |
3495-3497 |
IN |
denotes |
of |
T7266 |
3498-3503 |
NN |
denotes |
DMRT7 |
T7267 |
3504-3506 |
IN |
denotes |
by |
T7268 |
3507-3513 |
JJ |
denotes |
double |
T7269 |
3514-3522 |
NN |
denotes |
staining |
T7270 |
3523-3526 |
IN |
denotes |
for |
T7271 |
3527-3532 |
JJ |
denotes |
other |
T7272 |
3533-3540 |
NNS |
denotes |
markers |
T7273 |
3541-3550 |
VBG |
denotes |
including |
T7274 |
3551-3553 |
NN |
denotes |
Ub |
T7275 |
3554-3557 |
NN |
denotes |
H2A |
T7276 |
3553-3554 |
HYPH |
denotes |
- |
T7277 |
3558-3561 |
CC |
denotes |
and |
T7278 |
3562-3567 |
NN |
denotes |
γH2AX |
T7279 |
3568-3569 |
-LRB- |
denotes |
( |
T7280 |
3581-3585 |
NNS |
denotes |
data |
T7281 |
3569-3580 |
JJ |
denotes |
unpublished |
T7282 |
3585-3586 |
-RRB- |
denotes |
) |
T7283 |
3586-3587 |
. |
denotes |
. |
T7284 |
3587-3678 |
sentence |
denotes |
DMRT7 is not preferentially localized to the XY body at all stages but instead is dynamic. |
T7285 |
3588-3593 |
NN |
denotes |
DMRT7 |
T7286 |
3616-3625 |
VBN |
denotes |
localized |
T7287 |
3594-3596 |
VBZ |
denotes |
is |
T7288 |
3597-3600 |
RB |
denotes |
not |
T7289 |
3601-3615 |
RB |
denotes |
preferentially |
T7290 |
3626-3628 |
IN |
denotes |
to |
T7291 |
3629-3632 |
DT |
denotes |
the |
T7292 |
3636-3640 |
NN |
denotes |
body |
T7293 |
3633-3635 |
NN |
denotes |
XY |
T7294 |
3641-3643 |
IN |
denotes |
at |
T7295 |
3644-3647 |
DT |
denotes |
all |
T7296 |
3648-3654 |
NNS |
denotes |
stages |
T7297 |
3655-3658 |
CC |
denotes |
but |
T7298 |
3659-3666 |
RB |
denotes |
instead |
T7299 |
3667-3669 |
VBZ |
denotes |
is |
T7300 |
3670-3677 |
JJ |
denotes |
dynamic |
T7301 |
3677-3678 |
. |
denotes |
. |
T7302 |
3678-3880 |
sentence |
denotes |
Based on epithelial staging, it appears that DMRT7 localizes to the XY body from mid- to late-pachynema, becomes diffusely distributed in late-pachynema, and disappears in diplonema (unpublished data). |
T7303 |
3679-3684 |
VBN |
denotes |
Based |
T7304 |
3711-3718 |
VBZ |
denotes |
appears |
T7305 |
3685-3687 |
IN |
denotes |
on |
T7306 |
3688-3698 |
JJ |
denotes |
epithelial |
T7307 |
3699-3706 |
NN |
denotes |
staging |
T7308 |
3706-3708 |
, |
denotes |
, |
T7309 |
3708-3710 |
PRP |
denotes |
it |
T7310 |
3719-3723 |
IN |
denotes |
that |
T7311 |
3730-3739 |
VBZ |
denotes |
localizes |
T7312 |
3724-3729 |
NN |
denotes |
DMRT7 |
T7313 |
3740-3742 |
IN |
denotes |
to |
T7314 |
3743-3746 |
DT |
denotes |
the |
T7315 |
3750-3754 |
NN |
denotes |
body |
T7316 |
3747-3749 |
NN |
denotes |
XY |
T7317 |
3755-3759 |
IN |
denotes |
from |
T7318 |
3760-3763 |
JJ |
denotes |
mid |
T7319 |
3773-3782 |
NN |
denotes |
pachynema |
T7320 |
3763-3764 |
HYPH |
denotes |
- |
T7321 |
3765-3767 |
IN |
denotes |
to |
T7322 |
3768-3772 |
JJ |
denotes |
late |
T7323 |
3772-3773 |
HYPH |
denotes |
- |
T7324 |
3782-3784 |
, |
denotes |
, |
T7325 |
3784-3791 |
VBZ |
denotes |
becomes |
T7326 |
3792-3801 |
RB |
denotes |
diffusely |
T7327 |
3802-3813 |
JJ |
denotes |
distributed |
T7328 |
3814-3816 |
IN |
denotes |
in |
T7329 |
3817-3821 |
JJ |
denotes |
late |
T7330 |
3822-3831 |
NN |
denotes |
pachynema |
T7331 |
3821-3822 |
HYPH |
denotes |
- |
T7332 |
3831-3833 |
, |
denotes |
, |
T7333 |
3833-3836 |
CC |
denotes |
and |
T7334 |
3837-3847 |
VBZ |
denotes |
disappears |
T7335 |
3848-3850 |
IN |
denotes |
in |
T7336 |
3851-3860 |
NN |
denotes |
diplonema |
T7337 |
3861-3862 |
-LRB- |
denotes |
( |
T7338 |
3874-3878 |
NNS |
denotes |
data |
T7339 |
3862-3873 |
JJ |
denotes |
unpublished |
T7340 |
3878-3879 |
-RRB- |
denotes |
) |
T7341 |
3879-3880 |
. |
denotes |
. |
T7342 |
3880-3956 |
sentence |
denotes |
This localization was confirmed by staining of meiotic spreads (Figure S1). |
T7343 |
3881-3885 |
DT |
denotes |
This |
T7344 |
3886-3898 |
NN |
denotes |
localization |
T7345 |
3903-3912 |
VBN |
denotes |
confirmed |
T7346 |
3899-3902 |
VBD |
denotes |
was |
T7347 |
3913-3915 |
IN |
denotes |
by |
T7348 |
3916-3924 |
NN |
denotes |
staining |
T7349 |
3925-3927 |
IN |
denotes |
of |
T7350 |
3928-3935 |
JJ |
denotes |
meiotic |
T7351 |
3936-3943 |
NNS |
denotes |
spreads |
T7352 |
3944-3945 |
-LRB- |
denotes |
( |
T7353 |
3952-3954 |
NN |
denotes |
S1 |
T7354 |
3945-3951 |
NN |
denotes |
Figure |
T7355 |
3954-3955 |
-RRB- |
denotes |
) |
T7356 |
3955-3956 |
. |
denotes |
. |
T7357 |
3956-4086 |
sentence |
denotes |
DMRT7 also is specifically localized in sperm, with antibody staining mainly in the perinuclear ring of the sperm head manchette. |
T7358 |
3957-3962 |
NN |
denotes |
DMRT7 |
T7359 |
3984-3993 |
VBN |
denotes |
localized |
T7360 |
3963-3967 |
RB |
denotes |
also |
T7361 |
3968-3970 |
VBZ |
denotes |
is |
T7362 |
3971-3983 |
RB |
denotes |
specifically |
T7363 |
3994-3996 |
IN |
denotes |
in |
T7364 |
3997-4002 |
NN |
denotes |
sperm |
T7365 |
4002-4004 |
, |
denotes |
, |
T7366 |
4004-4008 |
IN |
denotes |
with |
T7367 |
4009-4017 |
NN |
denotes |
antibody |
T7368 |
4018-4026 |
NN |
denotes |
staining |
T7369 |
4027-4033 |
RB |
denotes |
mainly |
T7370 |
4034-4036 |
IN |
denotes |
in |
T7371 |
4037-4040 |
DT |
denotes |
the |
T7372 |
4053-4057 |
NN |
denotes |
ring |
T7373 |
4041-4052 |
JJ |
denotes |
perinuclear |
T7374 |
4058-4060 |
IN |
denotes |
of |
T7375 |
4061-4064 |
DT |
denotes |
the |
T7376 |
4076-4085 |
NN |
denotes |
manchette |
T7377 |
4065-4070 |
NN |
denotes |
sperm |
T7378 |
4071-4075 |
NN |
denotes |
head |
T7379 |
4085-4086 |
. |
denotes |
. |
T7380 |
4086-4214 |
sentence |
denotes |
This staining coincided with the epithelial stages in which DMRT7 localizes to the XY body in spermatocytes (Figure 1C and 1D). |
T7381 |
4087-4091 |
DT |
denotes |
This |
T7382 |
4092-4100 |
NN |
denotes |
staining |
T7383 |
4101-4110 |
VBD |
denotes |
coincided |
T7384 |
4111-4115 |
IN |
denotes |
with |
T7385 |
4116-4119 |
DT |
denotes |
the |
T7386 |
4131-4137 |
NNS |
denotes |
stages |
T7387 |
4120-4130 |
JJ |
denotes |
epithelial |
T7388 |
4138-4140 |
IN |
denotes |
in |
T7389 |
4153-4162 |
VBZ |
denotes |
localizes |
T7390 |
4141-4146 |
WDT |
denotes |
which |
T7391 |
4147-4152 |
NN |
denotes |
DMRT7 |
T7392 |
4163-4165 |
IN |
denotes |
to |
T7393 |
4166-4169 |
DT |
denotes |
the |
T7394 |
4173-4177 |
NN |
denotes |
body |
T7395 |
4170-4172 |
NN |
denotes |
XY |
T7396 |
4178-4180 |
IN |
denotes |
in |
T7397 |
4181-4194 |
NNS |
denotes |
spermatocytes |
T7398 |
4195-4196 |
-LRB- |
denotes |
( |
T7399 |
4203-4205 |
NN |
denotes |
1C |
T7400 |
4196-4202 |
NN |
denotes |
Figure |
T7401 |
4206-4209 |
CC |
denotes |
and |
T7402 |
4210-4212 |
NN |
denotes |
1D |
T7403 |
4212-4213 |
-RRB- |
denotes |
) |
T7404 |
4213-4214 |
. |
denotes |
. |
T27915 |
1664-1665 |
HYPH |
denotes |
- |
T27803 |
1206-1207 |
-LRB- |
denotes |
( |
T27759 |
1014-1024 |
NN |
denotes |
Expression |
T27760 |
1025-1027 |
IN |
denotes |
of |
T27761 |
1028-1033 |
NN |
denotes |
Dmrt7 |
T27762 |
1034-1038 |
NN |
denotes |
mRNA |
T27763 |
1039-1042 |
CC |
denotes |
and |
T27764 |
1043-1050 |
NN |
denotes |
Protein |
T27765 |
1050-1116 |
sentence |
denotes |
(A) RT-PCR analysis of Dmrt7 mRNA from ten organs of adult mouse. |
T27766 |
1051-1052 |
-LRB- |
denotes |
( |
T27767 |
1052-1053 |
LS |
denotes |
A |
T27768 |
1062-1070 |
NN |
denotes |
analysis |
T27769 |
1053-1054 |
-RRB- |
denotes |
) |
T27770 |
1055-1057 |
NN |
denotes |
RT |
T27771 |
1058-1061 |
NN |
denotes |
PCR |
T27772 |
1057-1058 |
HYPH |
denotes |
- |
T27773 |
1071-1073 |
IN |
denotes |
of |
T27774 |
1074-1079 |
NN |
denotes |
Dmrt7 |
T27775 |
1080-1084 |
NN |
denotes |
mRNA |
T27776 |
1085-1089 |
IN |
denotes |
from |
T27777 |
1090-1093 |
CD |
denotes |
ten |
T27778 |
1094-1100 |
NNS |
denotes |
organs |
T27779 |
1101-1103 |
IN |
denotes |
of |
T27780 |
1104-1109 |
JJ |
denotes |
adult |
T27781 |
1110-1115 |
NN |
denotes |
mouse |
T27782 |
1115-1116 |
. |
denotes |
. |
T27783 |
1116-1219 |
sentence |
denotes |
cDNA from each organ was amplified with primers specific for Dmrt7 (top row) and β-actin (bottom row). |
T27784 |
1117-1121 |
NN |
denotes |
cDNA |
T27785 |
1142-1151 |
VBN |
denotes |
amplified |
T27786 |
1122-1126 |
IN |
denotes |
from |
T27787 |
1127-1131 |
DT |
denotes |
each |
T27788 |
1132-1137 |
NN |
denotes |
organ |
T27789 |
1138-1141 |
VBD |
denotes |
was |
T27790 |
1152-1156 |
IN |
denotes |
with |
T27791 |
1157-1164 |
NNS |
denotes |
primers |
T27792 |
1165-1173 |
JJ |
denotes |
specific |
T27793 |
1174-1177 |
IN |
denotes |
for |
T27794 |
1178-1183 |
NN |
denotes |
Dmrt7 |
T27795 |
1184-1185 |
-LRB- |
denotes |
( |
T27796 |
1189-1192 |
NN |
denotes |
row |
T27797 |
1185-1188 |
JJ |
denotes |
top |
T27798 |
1192-1193 |
-RRB- |
denotes |
) |
T27799 |
1194-1197 |
CC |
denotes |
and |
T27800 |
1198-1199 |
NN |
denotes |
β |
T27801 |
1200-1205 |
NN |
denotes |
actin |
T27802 |
1199-1200 |
HYPH |
denotes |
- |
T27804 |
1214-1217 |
NN |
denotes |
row |
T27805 |
1207-1213 |
JJ |
denotes |
bottom |
T27806 |
1217-1218 |
-RRB- |
denotes |
) |
T27807 |
1218-1219 |
. |
denotes |
. |
T27808 |
1219-1288 |
sentence |
denotes |
(B) Dmrt7 mRNA expression during the first round of spermatogenesis. |
T27809 |
1220-1221 |
-LRB- |
denotes |
( |
T27810 |
1221-1222 |
LS |
denotes |
B |
T27811 |
1235-1245 |
NN |
denotes |
expression |
T27812 |
1222-1223 |
-RRB- |
denotes |
) |
T27813 |
1224-1229 |
NN |
denotes |
Dmrt7 |
T27814 |
1230-1234 |
NN |
denotes |
mRNA |
T27815 |
1246-1252 |
IN |
denotes |
during |
T27816 |
1253-1256 |
DT |
denotes |
the |
T27817 |
1263-1268 |
NN |
denotes |
round |
T27818 |
1257-1262 |
JJ |
denotes |
first |
T27819 |
1269-1271 |
IN |
denotes |
of |
T27820 |
1272-1287 |
NN |
denotes |
spermatogenesis |
T27821 |
1287-1288 |
. |
denotes |
. |
T27822 |
1288-1375 |
sentence |
denotes |
cDNAs obtained from testis at the indicated days after birth were amplified as in (A). |
T27823 |
1289-1294 |
NNS |
denotes |
cDNAs |
T27824 |
1355-1364 |
VBN |
denotes |
amplified |
T27825 |
1295-1303 |
VBN |
denotes |
obtained |
T27826 |
1304-1308 |
IN |
denotes |
from |
T27827 |
1309-1315 |
NN |
denotes |
testis |
T27828 |
1316-1318 |
IN |
denotes |
at |
T27829 |
1319-1322 |
DT |
denotes |
the |
T27830 |
1333-1337 |
NNS |
denotes |
days |
T27831 |
1323-1332 |
VBN |
denotes |
indicated |
T27832 |
1338-1343 |
IN |
denotes |
after |
T27833 |
1344-1349 |
NN |
denotes |
birth |
T27834 |
1350-1354 |
VBD |
denotes |
were |
T27835 |
1365-1367 |
IN |
denotes |
as |
T27836 |
1368-1370 |
IN |
denotes |
in |
T27837 |
1371-1372 |
-LRB- |
denotes |
( |
T27838 |
1372-1373 |
NN |
denotes |
A |
T27839 |
1373-1374 |
-RRB- |
denotes |
) |
T27840 |
1374-1375 |
. |
denotes |
. |
T27841 |
1375-1405 |
sentence |
denotes |
(C) DMRT7 protein expression. |
T27842 |
1376-1377 |
-LRB- |
denotes |
( |
T27843 |
1377-1378 |
LS |
denotes |
C |
T27844 |
1394-1404 |
NN |
denotes |
expression |
T27845 |
1378-1379 |
-RRB- |
denotes |
) |
T27846 |
1380-1385 |
NN |
denotes |
DMRT7 |
T27847 |
1386-1393 |
NN |
denotes |
protein |
T27848 |
1404-1405 |
. |
denotes |
. |
T27849 |
1405-1518 |
sentence |
denotes |
Immunofluorescence of testis sections from 6-wk-old male stained with antibody to DMRT7 (green) and DAPI (blue). |
T27850 |
1406-1424 |
NN |
denotes |
Immunofluorescence |
T27851 |
1425-1427 |
IN |
denotes |
of |
T27852 |
1428-1434 |
NN |
denotes |
testis |
T27853 |
1435-1443 |
NNS |
denotes |
sections |
T27854 |
1444-1448 |
IN |
denotes |
from |
T27855 |
1449-1450 |
CD |
denotes |
6 |
T27856 |
1451-1453 |
NN |
denotes |
wk |
T27857 |
1450-1451 |
HYPH |
denotes |
- |
T27858 |
1454-1457 |
JJ |
denotes |
old |
T27859 |
1453-1454 |
HYPH |
denotes |
- |
T27860 |
1458-1462 |
JJ |
denotes |
male |
T27861 |
1463-1470 |
VBN |
denotes |
stained |
T27862 |
1471-1475 |
IN |
denotes |
with |
T27863 |
1476-1484 |
NN |
denotes |
antibody |
T27864 |
1485-1487 |
IN |
denotes |
to |
T27865 |
1488-1493 |
NN |
denotes |
DMRT7 |
T27866 |
1494-1495 |
-LRB- |
denotes |
( |
T27867 |
1495-1500 |
JJ |
denotes |
green |
T27868 |
1500-1501 |
-RRB- |
denotes |
) |
T27869 |
1502-1505 |
CC |
denotes |
and |
T27870 |
1506-1510 |
NN |
denotes |
DAPI |
T27871 |
1511-1512 |
-LRB- |
denotes |
( |
T27872 |
1512-1516 |
JJ |
denotes |
blue |
T27873 |
1516-1517 |
-RRB- |
denotes |
) |
T27874 |
1517-1518 |
. |
denotes |
. |
T27875 |
1518-1565 |
sentence |
denotes |
(D) DMRT7 subcellular localization to XY body. |
T27876 |
1519-1520 |
-LRB- |
denotes |
( |
T27877 |
1520-1521 |
LS |
denotes |
D |
T27878 |
1541-1553 |
NN |
denotes |
localization |
T27879 |
1521-1522 |
-RRB- |
denotes |
) |
T27880 |
1523-1528 |
NN |
denotes |
DMRT7 |
T27881 |
1529-1540 |
JJ |
denotes |
subcellular |
T27882 |
1554-1556 |
IN |
denotes |
to |
T27883 |
1557-1559 |
NN |
denotes |
XY |
T27884 |
1560-1564 |
NN |
denotes |
body |
T27885 |
1564-1565 |
. |
denotes |
. |
T27886 |
1565-1659 |
sentence |
denotes |
Testis sections from 6-wk-old male stained with antibodies to DMRT7 (red) and SUMO-1 (green). |
T27887 |
1566-1572 |
NN |
denotes |
Testis |
T27888 |
1573-1581 |
NNS |
denotes |
sections |
T27889 |
1582-1586 |
IN |
denotes |
from |
T27890 |
1587-1588 |
CD |
denotes |
6 |
T27891 |
1589-1591 |
NN |
denotes |
wk |
T27892 |
1588-1589 |
HYPH |
denotes |
- |
T27893 |
1592-1595 |
JJ |
denotes |
old |
T27894 |
1591-1592 |
HYPH |
denotes |
- |
T27895 |
1596-1600 |
JJ |
denotes |
male |
T27896 |
1601-1608 |
VBN |
denotes |
stained |
T27897 |
1609-1613 |
IN |
denotes |
with |
T27898 |
1614-1624 |
NNS |
denotes |
antibodies |
T27899 |
1625-1627 |
IN |
denotes |
to |
T27900 |
1628-1633 |
NN |
denotes |
DMRT7 |
T27901 |
1634-1635 |
-LRB- |
denotes |
( |
T27902 |
1635-1638 |
JJ |
denotes |
red |
T27903 |
1638-1639 |
-RRB- |
denotes |
) |
T27904 |
1640-1643 |
CC |
denotes |
and |
T27905 |
1644-1648 |
NN |
denotes |
SUMO |
T27906 |
1648-1649 |
HYPH |
denotes |
- |
T27907 |
1649-1650 |
CD |
denotes |
1 |
T27908 |
1651-1652 |
-LRB- |
denotes |
( |
T27909 |
1652-1657 |
JJ |
denotes |
green |
T27910 |
1657-1658 |
-RRB- |
denotes |
) |
T27911 |
1658-1659 |
. |
denotes |
. |
T27912 |
1659-1695 |
sentence |
denotes |
SUMO-1 is localized to the XY body. |
T27913 |
1660-1664 |
NN |
denotes |
SUMO |
T27914 |
1670-1679 |
VBN |
denotes |
localized |
T27916 |
1665-1666 |
CD |
denotes |
1 |
T27917 |
1667-1669 |
VBZ |
denotes |
is |
T27918 |
1680-1682 |
IN |
denotes |
to |
T27919 |
1683-1686 |
DT |
denotes |
the |
T27920 |
1690-1694 |
NN |
denotes |
body |
T27921 |
1687-1689 |
NN |
denotes |
XY |
T27922 |
1694-1695 |
. |
denotes |
. |
T27923 |
1695-1745 |
sentence |
denotes |
Right-most panel shows merge of other two panels. |
T27924 |
1696-1706 |
JJ |
denotes |
Right-most |
T27925 |
1707-1712 |
NN |
denotes |
panel |
T27926 |
1713-1718 |
VBZ |
denotes |
shows |
T27927 |
1719-1724 |
NN |
denotes |
merge |
T27928 |
1725-1727 |
IN |
denotes |
of |
T27929 |
1728-1733 |
JJ |
denotes |
other |
T27930 |
1738-1744 |
NNS |
denotes |
panels |
T27931 |
1734-1737 |
CD |
denotes |
two |
T27932 |
1744-1745 |
. |
denotes |
. |
T27933 |
1745-1822 |
sentence |
denotes |
Inserts show higher magnification of pachytene spermatocytes with XY bodies. |
T27934 |
1746-1753 |
NNS |
denotes |
Inserts |
T27935 |
1754-1758 |
VBP |
denotes |
show |
T27936 |
1759-1765 |
JJR |
denotes |
higher |
T27937 |
1766-1779 |
NN |
denotes |
magnification |
T27938 |
1780-1782 |
IN |
denotes |
of |
T27939 |
1783-1792 |
NN |
denotes |
pachytene |
T27940 |
1793-1806 |
NNS |
denotes |
spermatocytes |
T27941 |
1807-1811 |
IN |
denotes |
with |
T27942 |
1812-1814 |
NN |
denotes |
XY |
T27943 |
1815-1821 |
NNS |
denotes |
bodies |
T27944 |
1821-1822 |
. |
denotes |
. |
T6854 |
499-504 |
NN |
denotes |
Dmrt7 |
T6855 |
515-517 |
, |
denotes |
, |
R7704 |
T27760 |
T27759 |
prep |
of,Expression |
R7705 |
T27761 |
T27762 |
compound |
Dmrt7,mRNA |
R7706 |
T27762 |
T27760 |
pobj |
mRNA,of |
R7707 |
T27763 |
T27762 |
cc |
and,mRNA |
R7708 |
T27764 |
T27762 |
conj |
Protein,mRNA |
R7709 |
T27766 |
T27767 |
punct |
(,A |
R7710 |
T27767 |
T27768 |
meta |
A,analysis |
R7711 |
T27769 |
T27767 |
punct |
),A |
R7712 |
T27770 |
T27771 |
compound |
RT,PCR |
R7713 |
T27771 |
T27768 |
compound |
PCR,analysis |
R7714 |
T27772 |
T27771 |
punct |
-,PCR |
R7715 |
T27773 |
T27768 |
prep |
of,analysis |
R7716 |
T27774 |
T27775 |
compound |
Dmrt7,mRNA |
R7717 |
T27775 |
T27773 |
pobj |
mRNA,of |
R7718 |
T27776 |
T27775 |
prep |
from,mRNA |
R7719 |
T27777 |
T27778 |
nummod |
ten,organs |
R7720 |
T27778 |
T27776 |
pobj |
organs,from |
R7721 |
T27779 |
T27778 |
prep |
of,organs |
R7722 |
T27780 |
T27781 |
amod |
adult,mouse |
R7723 |
T27781 |
T27779 |
pobj |
mouse,of |
R7724 |
T27782 |
T27768 |
punct |
.,analysis |
R7725 |
T27784 |
T27785 |
nsubjpass |
cDNA,amplified |
R7726 |
T27786 |
T27784 |
prep |
from,cDNA |
R7727 |
T27787 |
T27788 |
det |
each,organ |
R7728 |
T27788 |
T27786 |
pobj |
organ,from |
R7729 |
T27789 |
T27785 |
auxpass |
was,amplified |
R7730 |
T27790 |
T27785 |
prep |
with,amplified |
R7731 |
T27791 |
T27790 |
pobj |
primers,with |
R7732 |
T27792 |
T27791 |
amod |
specific,primers |
R7733 |
T27793 |
T27792 |
prep |
for,specific |
R7734 |
T27794 |
T27793 |
pobj |
Dmrt7,for |
R7735 |
T27795 |
T27796 |
punct |
(,row |
R7736 |
T27796 |
T27794 |
parataxis |
row,Dmrt7 |
R7737 |
T27797 |
T27796 |
amod |
top,row |
R7738 |
T27798 |
T27796 |
punct |
),row |
R7739 |
T27799 |
T27794 |
cc |
and,Dmrt7 |
R7740 |
T27800 |
T27801 |
compound |
β,actin |
R7741 |
T27801 |
T27794 |
conj |
actin,Dmrt7 |
R7742 |
T27802 |
T27801 |
punct |
-,actin |
R7743 |
T27803 |
T27804 |
punct |
(,row |
R7744 |
T27804 |
T27801 |
parataxis |
row,actin |
R7745 |
T27805 |
T27804 |
amod |
bottom,row |
R7746 |
T27806 |
T27804 |
punct |
),row |
R7747 |
T27807 |
T27785 |
punct |
.,amplified |
R7748 |
T27809 |
T27810 |
punct |
(,B |
R7749 |
T27810 |
T27811 |
meta |
B,expression |
R7750 |
T27812 |
T27810 |
punct |
),B |
R7751 |
T27813 |
T27811 |
compound |
Dmrt7,expression |
R7752 |
T27814 |
T27811 |
compound |
mRNA,expression |
R7753 |
T27815 |
T27811 |
prep |
during,expression |
R7754 |
T27816 |
T27817 |
det |
the,round |
R7755 |
T27817 |
T27815 |
pobj |
round,during |
R7756 |
T27818 |
T27817 |
amod |
first,round |
R7757 |
T27819 |
T27817 |
prep |
of,round |
R7758 |
T27820 |
T27819 |
pobj |
spermatogenesis,of |
R7759 |
T27821 |
T27811 |
punct |
.,expression |
R7760 |
T27823 |
T27824 |
nsubjpass |
cDNAs,amplified |
R7761 |
T27825 |
T27823 |
acl |
obtained,cDNAs |
R7762 |
T27826 |
T27825 |
prep |
from,obtained |
R7763 |
T27827 |
T27826 |
pobj |
testis,from |
R7764 |
T27828 |
T27825 |
prep |
at,obtained |
R7765 |
T27829 |
T27830 |
det |
the,days |
R7766 |
T27830 |
T27828 |
pobj |
days,at |
R7767 |
T27831 |
T27830 |
amod |
indicated,days |
R7768 |
T27832 |
T27830 |
prep |
after,days |
R7769 |
T27833 |
T27832 |
pobj |
birth,after |
R7770 |
T27834 |
T27824 |
auxpass |
were,amplified |
R7771 |
T27835 |
T27824 |
prep |
as,amplified |
R7772 |
T27836 |
T27835 |
prep |
in,as |
R7773 |
T27837 |
T27836 |
punct |
(,in |
R7774 |
T27838 |
T27836 |
pobj |
A,in |
R7775 |
T27839 |
T27824 |
punct |
),amplified |
R7776 |
T27840 |
T27824 |
punct |
.,amplified |
R7777 |
T27842 |
T27843 |
punct |
(,C |
R7778 |
T27843 |
T27844 |
meta |
C,expression |
R7779 |
T27845 |
T27843 |
punct |
),C |
R7780 |
T27846 |
T27844 |
compound |
DMRT7,expression |
R7781 |
T27847 |
T27844 |
compound |
protein,expression |
R7782 |
T27848 |
T27844 |
punct |
.,expression |
R7783 |
T27851 |
T27850 |
prep |
of,Immunofluorescence |
R7784 |
T27852 |
T27853 |
compound |
testis,sections |
R7785 |
T27853 |
T27851 |
pobj |
sections,of |
R7786 |
T27854 |
T27853 |
prep |
from,sections |
R7787 |
T27855 |
T27856 |
nummod |
6,wk |
R7788 |
T27856 |
T27858 |
npadvmod |
wk,old |
R7789 |
T27857 |
T27856 |
punct |
-,wk |
R7790 |
T27858 |
T27860 |
amod |
old,male |
R7791 |
T27859 |
T27858 |
punct |
-,old |
R7792 |
T27860 |
T27854 |
pobj |
male,from |
R7793 |
T27861 |
T27850 |
acl |
stained,Immunofluorescence |
R7794 |
T27862 |
T27861 |
prep |
with,stained |
R7795 |
T27863 |
T27862 |
pobj |
antibody,with |
R7796 |
T27864 |
T27863 |
prep |
to,antibody |
R7797 |
T27865 |
T27864 |
pobj |
DMRT7,to |
R7798 |
T27866 |
T27867 |
punct |
(,green |
R7799 |
T27867 |
T27865 |
parataxis |
green,DMRT7 |
R7800 |
T27868 |
T27867 |
punct |
),green |
R7801 |
T27869 |
T27865 |
cc |
and,DMRT7 |
R7802 |
T27870 |
T27865 |
conj |
DAPI,DMRT7 |
R7803 |
T27871 |
T27872 |
punct |
(,blue |
R7804 |
T27872 |
T27870 |
parataxis |
blue,DAPI |
R7805 |
T27873 |
T27872 |
punct |
),blue |
R7806 |
T27874 |
T27850 |
punct |
.,Immunofluorescence |
R7807 |
T27876 |
T27877 |
punct |
(,D |
R7808 |
T27877 |
T27878 |
meta |
D,localization |
R7809 |
T27879 |
T27878 |
punct |
),localization |
R7810 |
T27880 |
T27878 |
nmod |
DMRT7,localization |
R7811 |
T27881 |
T27878 |
amod |
subcellular,localization |
R7812 |
T27882 |
T27878 |
prep |
to,localization |
R7813 |
T27883 |
T27884 |
compound |
XY,body |
R7814 |
T27884 |
T27882 |
pobj |
body,to |
R7815 |
T27885 |
T27878 |
punct |
.,localization |
R7816 |
T27887 |
T27888 |
compound |
Testis,sections |
R7817 |
T27889 |
T27888 |
prep |
from,sections |
R7818 |
T27890 |
T27891 |
nummod |
6,wk |
R7819 |
T27891 |
T27893 |
npadvmod |
wk,old |
R7820 |
T27892 |
T27891 |
punct |
-,wk |
R7821 |
T27893 |
T27895 |
amod |
old,male |
R7822 |
T27894 |
T27893 |
punct |
-,old |
R7823 |
T27895 |
T27889 |
pobj |
male,from |
R7824 |
T27896 |
T27888 |
acl |
stained,sections |
R7825 |
T27897 |
T27896 |
prep |
with,stained |
R7826 |
T27898 |
T27897 |
pobj |
antibodies,with |
R7827 |
T27899 |
T27898 |
prep |
to,antibodies |
R7828 |
T27900 |
T27899 |
pobj |
DMRT7,to |
R7829 |
T27901 |
T27902 |
punct |
(,red |
R7830 |
T27902 |
T27900 |
parataxis |
red,DMRT7 |
R7831 |
T27903 |
T27902 |
punct |
),red |
R7832 |
T27904 |
T27900 |
cc |
and,DMRT7 |
R7833 |
T27905 |
T27900 |
conj |
SUMO,DMRT7 |
R7834 |
T27906 |
T27905 |
punct |
-,SUMO |
R7835 |
T27907 |
T27905 |
nummod |
1,SUMO |
R7836 |
T27908 |
T27909 |
punct |
(,green |
R7837 |
T27909 |
T27905 |
parataxis |
green,SUMO |
R7838 |
T27910 |
T27909 |
punct |
),green |
R7839 |
T27911 |
T27888 |
punct |
.,sections |
R7840 |
T27913 |
T27914 |
nsubjpass |
SUMO,localized |
R7841 |
T27915 |
T27913 |
punct |
-,SUMO |
R7842 |
T27916 |
T27913 |
nummod |
1,SUMO |
R7843 |
T27917 |
T27914 |
auxpass |
is,localized |
R7844 |
T27918 |
T27914 |
prep |
to,localized |
R7845 |
T27919 |
T27920 |
det |
the,body |
R7846 |
T27920 |
T27918 |
pobj |
body,to |
R7847 |
T27921 |
T27920 |
compound |
XY,body |
R7848 |
T27922 |
T27914 |
punct |
.,localized |
R7849 |
T27924 |
T27925 |
amod |
Right-most,panel |
R7850 |
T27925 |
T27926 |
nsubj |
panel,shows |
R7851 |
T27927 |
T27926 |
dobj |
merge,shows |
R7852 |
T27928 |
T27927 |
prep |
of,merge |
R7853 |
T27929 |
T27930 |
amod |
other,panels |
R7854 |
T27930 |
T27928 |
pobj |
panels,of |
R7855 |
T27931 |
T27930 |
nummod |
two,panels |
R7856 |
T27932 |
T27926 |
punct |
.,shows |
R7857 |
T27934 |
T27935 |
nsubj |
Inserts,show |
R7858 |
T27936 |
T27937 |
amod |
higher,magnification |
R7859 |
T27937 |
T27935 |
dobj |
magnification,show |
R7860 |
T27938 |
T27937 |
prep |
of,magnification |
R7861 |
T27939 |
T27940 |
compound |
pachytene,spermatocytes |
R7862 |
T27940 |
T27938 |
pobj |
spermatocytes,of |
R7863 |
T27941 |
T27940 |
prep |
with,spermatocytes |
R7864 |
T27942 |
T27943 |
compound |
XY,bodies |
R7865 |
T27943 |
T27941 |
pobj |
bodies,with |
R7866 |
T27944 |
T27935 |
punct |
.,show |
R1459 |
T6760 |
T6759 |
prep |
of,Expression |
R1460 |
T6761 |
T6762 |
compound |
DMRT7,Proteins |
R1461 |
T6762 |
T6760 |
pobj |
Proteins,of |
R1462 |
T6763 |
T6759 |
prep |
in,Expression |
R1463 |
T6764 |
T6763 |
pobj |
Testis,in |
R1464 |
T6766 |
T6767 |
poss |
Our,analysis |
R1465 |
T6767 |
T6771 |
nsubj |
analysis,suggested |
R1466 |
T6768 |
T6767 |
amod |
previous,analysis |
R1467 |
T6769 |
T6770 |
compound |
mRNA,expression |
R1468 |
T6770 |
T6767 |
compound |
expression,analysis |
R1469 |
T6772 |
T6773 |
det |
a,function |
R1470 |
T6773 |
T6771 |
dobj |
function,suggested |
R1471 |
T6774 |
T6773 |
amod |
possible,function |
R1472 |
T6775 |
T6773 |
amod |
meiotic,function |
R1473 |
T6776 |
T6773 |
prep |
for,function |
R1474 |
T6777 |
T6776 |
pobj |
Dmrt7,for |
R1475 |
T6778 |
T6771 |
punct |
", ",suggested |
R1476 |
T6779 |
T6771 |
prep |
based,suggested |
R1477 |
T6780 |
T6779 |
prep |
on,based |
R1478 |
T6781 |
T6782 |
det |
the,expression |
R1479 |
T6782 |
T6780 |
pobj |
expression,on |
R1480 |
T6783 |
T6782 |
prep |
of,expression |
R1481 |
T6784 |
T6785 |
compound |
Dmrt7,mRNA |
R1482 |
T6785 |
T6783 |
pobj |
mRNA,of |
R1483 |
T6786 |
T6782 |
prep |
in,expression |
R1484 |
T6787 |
T6788 |
det |
the,gonads |
R1485 |
T6788 |
T6786 |
pobj |
gonads,in |
R1486 |
T6789 |
T6788 |
amod |
fetal,gonads |
R1487 |
T6790 |
T6788 |
prep |
of,gonads |
R1488 |
T6791 |
T6792 |
det |
the,sexes |
R1489 |
T6792 |
T6790 |
pobj |
sexes,of |
R1490 |
T6793 |
T6792 |
nummod |
two,sexes |
R1491 |
T6794 |
T6795 |
punct |
[,23 |
R1492 |
T6795 |
T6771 |
parataxis |
23,suggested |
R1493 |
T6796 |
T6795 |
punct |
],23 |
R1494 |
T6797 |
T6771 |
punct |
.,suggested |
R1495 |
T6799 |
T6800 |
prep |
In,detected |
R1496 |
T6801 |
T6802 |
det |
the,ovary |
R1497 |
T6802 |
T6799 |
pobj |
ovary,In |
R1498 |
T6803 |
T6802 |
amod |
fetal,ovary |
R1499 |
T6804 |
T6800 |
punct |
", ",detected |
R1500 |
T6805 |
T6806 |
compound |
Dmrt7,mRNA |
R1501 |
T6806 |
T6800 |
nsubjpass |
mRNA,detected |
R1502 |
T6807 |
T6800 |
auxpass |
was,detected |
R1503 |
T6808 |
T6800 |
advmod |
primarily,detected |
R1504 |
T6809 |
T6800 |
prep |
from,detected |
R1505 |
T6810 |
T6809 |
pobj |
E13.5,from |
R1506 |
T6811 |
T6809 |
prep |
to,from |
R1507 |
T6812 |
T6811 |
pobj |
E15.5,to |
R1508 |
T6813 |
T6800 |
punct |
", ",detected |
R1509 |
T6814 |
T6815 |
det |
the,time |
R1510 |
T6815 |
T6800 |
npadvmod |
time,detected |
R1511 |
T6816 |
T6817 |
prep |
during,progresses |
R1512 |
T6817 |
T6815 |
relcl |
progresses,time |
R1513 |
T6818 |
T6816 |
pobj |
which,during |
R1514 |
T6819 |
T6817 |
nsubj |
meiosis,progresses |
R1515 |
T6820 |
T6817 |
prep |
from,progresses |
R1516 |
T6821 |
T6822 |
amod |
pre-meiotic,replication |
R1517 |
T6822 |
T6820 |
pobj |
replication,from |
R1518 |
T6823 |
T6820 |
prep |
to,from |
R1519 |
T6824 |
T6825 |
det |
the,stage |
R1520 |
T6825 |
T6823 |
pobj |
stage,to |
R1521 |
T6826 |
T6825 |
compound |
pachytene,stage |
R1522 |
T6827 |
T6828 |
punct |
[,4 |
R1523 |
T6828 |
T6800 |
parataxis |
4,detected |
R1524 |
T6829 |
T6828 |
punct |
],4 |
R1525 |
T6830 |
T6800 |
punct |
", ",detected |
R1526 |
T6831 |
T6832 |
mark |
whereas,was |
R1527 |
T6832 |
T6800 |
advcl |
was,detected |
R1528 |
T6833 |
T6834 |
compound |
Dmrt7,expression |
R1529 |
T6834 |
T6832 |
nsubj |
expression,was |
R1530 |
T6835 |
T6834 |
prep |
in,expression |
R1531 |
T6836 |
T6837 |
det |
the,testis |
R1532 |
T6837 |
T6835 |
pobj |
testis,in |
R1533 |
T6838 |
T6837 |
amod |
non-meiotic,testis |
R1534 |
T6839 |
T6837 |
amod |
fetal,testis |
R1535 |
T6840 |
T6841 |
advmod |
very,low |
R1536 |
T6841 |
T6832 |
acomp |
low,was |
R1537 |
T6842 |
T6800 |
punct |
.,detected |
R1538 |
T6844 |
T6845 |
mark |
Because,examine |
R1539 |
T6845 |
T6851 |
advcl |
examine,performed |
R1540 |
T6846 |
T6847 |
det |
this,work |
R1541 |
T6847 |
T6845 |
nsubj |
work,examine |
R1542 |
T6848 |
T6847 |
amod |
earlier,work |
R1543 |
T6849 |
T6845 |
aux |
did,examine |
R1544 |
T6850 |
T6845 |
neg |
not,examine |
R1545 |
T6852 |
T6853 |
amod |
adult,expression |
R1546 |
T6853 |
T6845 |
dobj |
expression,examine |
R1547 |
T6854 |
T6853 |
compound |
Dmrt7,expression |
R1548 |
T6855 |
T6851 |
punct |
", ",performed |
R1549 |
T6856 |
T6851 |
nsubj |
we,performed |
R1550 |
T6857 |
T6851 |
advmod |
first,performed |
R1551 |
T6858 |
T6859 |
amod |
reverse,transcriptase |
R1552 |
T6859 |
T6860 |
nmod |
transcriptase,PCR |
R1553 |
T6860 |
T6851 |
dobj |
PCR,performed |
R1554 |
T6861 |
T6859 |
punct |
(,transcriptase |
R1555 |
T6862 |
T6859 |
appos |
RT,transcriptase |
R1556 |
T6863 |
T6860 |
punct |
),PCR |
R1557 |
T6864 |
T6860 |
punct |
-,PCR |
R1558 |
T6865 |
T6860 |
prep |
on,PCR |
R1559 |
T6866 |
T6865 |
pobj |
mRNA,on |
R1560 |
T6867 |
T6866 |
prep |
from,mRNA |
R1561 |
T6868 |
T6869 |
nummod |
ten,organs |
R1562 |
T6869 |
T6867 |
pobj |
organs,from |
R1563 |
T6870 |
T6869 |
amod |
adult,organs |
R1564 |
T6871 |
T6851 |
cc |
and,performed |
R1565 |
T6872 |
T6851 |
conj |
detected,performed |
R1566 |
T6873 |
T6874 |
amod |
strong,expression |
R1567 |
T6874 |
T6872 |
dobj |
expression,detected |
R1568 |
T6875 |
T6874 |
compound |
Dmrt7,expression |
R1569 |
T6876 |
T6874 |
compound |
mRNA,expression |
R1570 |
T6877 |
T6874 |
prep |
in,expression |
R1571 |
T6878 |
T6879 |
det |
the,testis |
R1572 |
T6879 |
T6877 |
pobj |
testis,in |
R1573 |
T6880 |
T6874 |
cc |
and,expression |
R1574 |
T6881 |
T6882 |
det |
a,trace |
R1575 |
T6882 |
T6874 |
conj |
trace,expression |
R1576 |
T6883 |
T6882 |
prep |
of,trace |
R1577 |
T6884 |
T6883 |
pobj |
expression,of |
R1578 |
T6885 |
T6882 |
prep |
in,trace |
R1579 |
T6886 |
T6885 |
pobj |
heart,in |
R1580 |
T6887 |
T6885 |
punct |
", ",in |
R1581 |
T6888 |
T6885 |
cc |
but,in |
R1582 |
T6889 |
T6888 |
neg |
not,but |
R1583 |
T6890 |
T6885 |
conj |
in,in |
R1584 |
T6891 |
T6892 |
det |
any,tissue |
R1585 |
T6892 |
T6890 |
pobj |
tissue,in |
R1586 |
T6893 |
T6892 |
amod |
other,tissue |
R1587 |
T6894 |
T6892 |
acl |
tested,tissue |
R1588 |
T6895 |
T6896 |
punct |
(,1A |
R1589 |
T6896 |
T6872 |
parataxis |
1A,detected |
R1590 |
T6897 |
T6896 |
compound |
Figure,1A |
R1591 |
T6898 |
T6896 |
punct |
),1A |
R1592 |
T6899 |
T6851 |
punct |
.,performed |
R1593 |
T6901 |
T6902 |
nsubj |
We,examined |
R1594 |
T6903 |
T6904 |
det |
the,timing |
R1595 |
T6904 |
T6902 |
dobj |
timing,examined |
R1596 |
T6905 |
T6904 |
prep |
of,timing |
R1597 |
T6906 |
T6907 |
compound |
Dmrt7,expression |
R1598 |
T6907 |
T6905 |
pobj |
expression,of |
R1599 |
T6908 |
T6907 |
compound |
mRNA,expression |
R1600 |
T6909 |
T6904 |
prep |
during,timing |
R1601 |
T6910 |
T6911 |
amod |
postnatal,development |
R1602 |
T6911 |
T6909 |
pobj |
development,during |
R1603 |
T6912 |
T6911 |
compound |
testis,development |
R1604 |
T6913 |
T6902 |
cc |
and,examined |
R1605 |
T6914 |
T6902 |
conj |
detected,examined |
R1606 |
T6915 |
T6916 |
amod |
strong,expression |
R1607 |
T6916 |
T6914 |
dobj |
expression,detected |
R1608 |
T6917 |
T6916 |
acl |
beginning,expression |
R1609 |
T6918 |
T6917 |
prep |
at,beginning |
R1610 |
T6919 |
T6920 |
nummod |
2,wk |
R1611 |
T6920 |
T6918 |
pobj |
wk,at |
R1612 |
T6921 |
T6916 |
punct |
", ",expression |
R1613 |
T6922 |
T6923 |
dep |
which,coincides |
R1614 |
T6923 |
T6916 |
relcl |
coincides,expression |
R1615 |
T6924 |
T6923 |
advmod |
roughly,coincides |
R1616 |
T6925 |
T6923 |
prep |
with,coincides |
R1617 |
T6926 |
T6927 |
det |
the,onset |
R1618 |
T6927 |
T6925 |
pobj |
onset,with |
R1619 |
T6928 |
T6927 |
prep |
of,onset |
R1620 |
T6929 |
T6930 |
det |
the,stage |
R1621 |
T6930 |
T6928 |
pobj |
stage,of |
R1622 |
T6931 |
T6930 |
compound |
pachytene,stage |
R1623 |
T6932 |
T6927 |
prep |
during,onset |
R1625 |
T6934 |
T6932 |
pobj |
wave,during |
R1626 |
T6935 |
T6934 |
amod |
first,wave |
R1627 |
T6936 |
T6934 |
amod |
synchronous,wave |
R1628 |
T6937 |
T6934 |
prep |
of,wave |
R1629 |
T6938 |
T6937 |
pobj |
spermatogenesis,of |
R1630 |
T6939 |
T6940 |
punct |
(,1B |
R1631 |
T6940 |
T6923 |
parataxis |
1B,coincides |
R1632 |
T6941 |
T6940 |
compound |
Figure,1B |
R1633 |
T6942 |
T6940 |
punct |
),1B |
R1634 |
T6943 |
T6944 |
punct |
[,37 |
R1635 |
T6944 |
T6914 |
parataxis |
37,detected |
R1636 |
T6945 |
T6944 |
punct |
],37 |
R1637 |
T6946 |
T6902 |
punct |
.,examined |
R1638 |
T6949 |
T6950 |
aux |
To,investigate |
R1639 |
T6950 |
T6951 |
advcl |
investigate,generated |
R1640 |
T6952 |
T6953 |
compound |
DMRT7,expression |
R1641 |
T6953 |
T6950 |
dobj |
expression,investigate |
R1642 |
T6954 |
T6953 |
compound |
protein,expression |
R1643 |
T6955 |
T6951 |
punct |
", ",generated |
R1644 |
T6956 |
T6951 |
nsubj |
we,generated |
R1645 |
T6957 |
T6958 |
det |
an,antibody |
R1646 |
T6958 |
T6951 |
dobj |
antibody,generated |
R1647 |
T6959 |
T6958 |
prep |
against,antibody |
R1648 |
T6960 |
T6961 |
det |
the,portion |
R1649 |
T6961 |
T6959 |
pobj |
portion,against |
R1650 |
T6962 |
T6963 |
npadvmod |
C,terminal |
R1651 |
T6963 |
T6961 |
amod |
terminal,portion |
R1652 |
T6964 |
T6963 |
punct |
-,terminal |
R1653 |
T6965 |
T6961 |
prep |
of,portion |
R1654 |
T6966 |
T6967 |
det |
the,protein |
R1655 |
T6967 |
T6965 |
pobj |
protein,of |
R1656 |
T6968 |
T6951 |
punct |
.,generated |
R1657 |
T6970 |
T6971 |
det |
The,antibody |
R1658 |
T6971 |
T6972 |
nsubjpass |
antibody,raised |
R1659 |
T6973 |
T6972 |
auxpass |
was,raised |
R1660 |
T6974 |
T6972 |
prep |
against,raised |
R1661 |
T6975 |
T6976 |
det |
a,region |
R1662 |
T6976 |
T6974 |
pobj |
region,against |
R1663 |
T6977 |
T6976 |
amod |
unique,region |
R1664 |
T6978 |
T6976 |
acl |
lacking,region |
R1665 |
T6979 |
T6980 |
det |
the,domain |
R1666 |
T6980 |
T6978 |
dobj |
domain,lacking |
R1667 |
T6981 |
T6980 |
compound |
DM,domain |
R1668 |
T6982 |
T6972 |
prep |
in,raised |
R1669 |
T6983 |
T6982 |
pobj |
order,in |
R1670 |
T6984 |
T6985 |
aux |
to,avoid |
R1671 |
T6985 |
T6983 |
acl |
avoid,order |
R1672 |
T6986 |
T6985 |
dobj |
cross-reaction,avoid |
R1673 |
T6987 |
T6986 |
prep |
with,cross-reaction |
R1674 |
T6988 |
T6989 |
amod |
other,proteins |
R1675 |
T6989 |
T6987 |
pobj |
proteins,with |
R1676 |
T6990 |
T6991 |
compound |
DM,domain |
R1677 |
T6991 |
T6989 |
compound |
domain,proteins |
R1678 |
T6992 |
T6972 |
punct |
.,raised |
R1679 |
T6994 |
T6995 |
amod |
Immunofluorescent,staining |
R1680 |
T6995 |
T6996 |
nsubj |
staining,showed |
R1681 |
T6997 |
T6995 |
prep |
with,staining |
R1682 |
T6998 |
T6999 |
amod |
purified,antisera |
R1683 |
T6999 |
T6997 |
pobj |
antisera,with |
R1684 |
T7000 |
T6999 |
compound |
DMRT7,antisera |
R1685 |
T7001 |
T7002 |
mark |
that,expressed |
R1686 |
T7002 |
T6996 |
ccomp |
expressed,showed |
R1687 |
T7003 |
T7004 |
compound |
DMRT7,protein |
R1688 |
T7004 |
T7002 |
nsubjpass |
protein,expressed |
R1689 |
T7005 |
T7002 |
auxpass |
is,expressed |
R1690 |
T7006 |
T7002 |
advmod |
predominantly,expressed |
R1691 |
T7007 |
T7002 |
prep |
in,expressed |
R1692 |
T7008 |
T7009 |
amod |
mid,pachytene |
R1693 |
T7009 |
T7014 |
compound |
pachytene,spermatocytes |
R1694 |
T7010 |
T7008 |
punct |
-,mid |
R1695 |
T7011 |
T7008 |
prep |
to,mid |
R1696 |
T7012 |
T7011 |
amod |
late,to |
R1697 |
T7013 |
T7009 |
punct |
-,pachytene |
R1698 |
T7014 |
T7007 |
pobj |
spermatocytes,in |
R1699 |
T7015 |
T7016 |
punct |
(,1C |
R1700 |
T7016 |
T7014 |
parataxis |
1C,spermatocytes |
R1701 |
T7017 |
T7016 |
compound |
Figure,1C |
R1702 |
T7018 |
T7016 |
punct |
),1C |
R1703 |
T7019 |
T7007 |
punct |
", ",in |
R1704 |
T7020 |
T7021 |
advmod |
as,as |
R1705 |
T7021 |
T7007 |
cc |
as,in |
R1706 |
T7022 |
T7021 |
advmod |
well,as |
R1707 |
T7023 |
T7007 |
conj |
in,in |
R1708 |
T7024 |
T7023 |
pobj |
sperm,in |
R1709 |
T7025 |
T7002 |
punct |
", ",expressed |
R1710 |
T7026 |
T7002 |
cc |
and,expressed |
R1711 |
T7027 |
T7002 |
conj |
is,expressed |
R1712 |
T7028 |
T7027 |
neg |
not,is |
R1713 |
T7029 |
T7027 |
acomp |
detectable,is |
R1714 |
T7030 |
T7027 |
prep |
in,is |
R1715 |
T7031 |
T7032 |
amod |
other,types |
R1716 |
T7032 |
T7030 |
pobj |
types,in |
R1717 |
T7033 |
T7034 |
compound |
germ,cell |
R1718 |
T7034 |
T7032 |
compound |
cell,types |
R1719 |
T7035 |
T7032 |
prep |
including,types |
R1720 |
T7036 |
T7035 |
pobj |
spermatogonia,including |
R1721 |
T7037 |
T7036 |
cc |
and,spermatogonia |
R1722 |
T7038 |
T7039 |
amod |
round,spermatids |
R1723 |
T7039 |
T7036 |
conj |
spermatids,spermatogonia |
R1724 |
T7040 |
T6996 |
punct |
.,showed |
R1725 |
T7042 |
T7043 |
nsubj |
We,detect |
R1726 |
T7044 |
T7043 |
aux |
did,detect |
R1727 |
T7045 |
T7043 |
neg |
not,detect |
R1728 |
T7046 |
T7047 |
compound |
DMRT7,protein |
R1729 |
T7047 |
T7043 |
dobj |
protein,detect |
R1730 |
T7048 |
T7043 |
prep |
in,detect |
R1731 |
T7049 |
T7050 |
amod |
somatic,cells |
R1732 |
T7050 |
T7048 |
pobj |
cells,in |
R1733 |
T7051 |
T7052 |
amod |
such,as |
R1734 |
T7052 |
T7050 |
prep |
as,cells |
R1735 |
T7053 |
T7054 |
compound |
Sertoli,cells |
R1736 |
T7054 |
T7052 |
pobj |
cells,as |
R1737 |
T7055 |
T7054 |
punct |
", ",cells |
R1738 |
T7056 |
T7057 |
amod |
peritubular,cells |
R1739 |
T7057 |
T7054 |
conj |
cells,cells |
R1740 |
T7058 |
T7057 |
amod |
myoid,cells |
R1741 |
T7059 |
T7057 |
punct |
", ",cells |
R1742 |
T7060 |
T7057 |
cc |
or,cells |
R1743 |
T7061 |
T7062 |
compound |
Leydig,cells |
R1744 |
T7062 |
T7057 |
conj |
cells,cells |
R1745 |
T7063 |
T7043 |
punct |
.,detect |
R1746 |
T7065 |
T7066 |
aux |
To,determine |
R1747 |
T7066 |
T7069 |
advcl |
determine,stained |
R1748 |
T7067 |
T7068 |
advmod |
more,precisely |
R1749 |
T7068 |
T7066 |
advmod |
precisely,determine |
R1750 |
T7070 |
T7071 |
det |
the,stages |
R1751 |
T7071 |
T7066 |
dobj |
stages,determine |
R1752 |
T7072 |
T7071 |
compound |
pachytene,stages |
R1753 |
T7073 |
T7071 |
prep |
of,stages |
R1754 |
T7074 |
T7075 |
compound |
DMRT7,expression |
R1755 |
T7075 |
T7073 |
pobj |
expression,of |
R1756 |
T7076 |
T7069 |
punct |
", ",stained |
R1757 |
T7077 |
T7069 |
nsubj |
we,stained |
R1758 |
T7078 |
T7069 |
advmod |
double,stained |
R1759 |
T7079 |
T7069 |
punct |
-,stained |
R1760 |
T7080 |
T7069 |
prep |
with,stained |
R1761 |
T7081 |
T7082 |
det |
an,antibody |
R1762 |
T7082 |
T7080 |
pobj |
antibody,with |
R1763 |
T7083 |
T7082 |
prep |
to,antibody |
R1764 |
T7084 |
T7083 |
pobj |
GATA1,to |
R1765 |
T7085 |
T7084 |
punct |
", ",GATA1 |
R1766 |
T7086 |
T7087 |
dep |
which,expressed |
R1767 |
T7087 |
T7084 |
relcl |
expressed,GATA1 |
R1768 |
T7088 |
T7087 |
auxpass |
is,expressed |
R1769 |
T7089 |
T7087 |
prep |
in,expressed |
R1770 |
T7090 |
T7091 |
compound |
Sertoli,cells |
R1771 |
T7091 |
T7089 |
pobj |
cells,in |
R1772 |
T7092 |
T7091 |
prep |
from,cells |
R1773 |
T7093 |
T7094 |
nmod |
stages,VII |
R1774 |
T7094 |
T7092 |
pobj |
VII,from |
R1775 |
T7095 |
T7094 |
prep |
to,VII |
R1776 |
T7096 |
T7095 |
pobj |
IX,to |
R1777 |
T7097 |
T7098 |
punct |
[,38 |
R1778 |
T7098 |
T7069 |
parataxis |
38,stained |
R1779 |
T7099 |
T7098 |
punct |
],38 |
R1780 |
T7100 |
T7069 |
punct |
.,stained |
R1781 |
T7102 |
T7103 |
nsubj |
This,confirmed |
R1782 |
T7104 |
T7105 |
mark |
that,expressed |
R1783 |
T7105 |
T7103 |
ccomp |
expressed,confirmed |
R1784 |
T7106 |
T7105 |
nsubjpass |
DMRT7,expressed |
R1785 |
T7107 |
T7105 |
auxpass |
is,expressed |
R1786 |
T7108 |
T7105 |
prep |
in,expressed |
R1787 |
T7109 |
T7110 |
amod |
mid,pachytene |
R1788 |
T7110 |
T7115 |
compound |
pachytene,spermatocytes |
R1789 |
T7111 |
T7109 |
punct |
-,mid |
R1790 |
T7112 |
T7109 |
prep |
to,mid |
R1791 |
T7113 |
T7112 |
amod |
late,to |
R1792 |
T7114 |
T7110 |
punct |
-,pachytene |
R1793 |
T7115 |
T7108 |
pobj |
spermatocytes,in |
R1794 |
T7116 |
T7105 |
punct |
", ",expressed |
R1795 |
T7117 |
T7105 |
advcl |
starting,expressed |
R1796 |
T7118 |
T7119 |
advmod |
slightly,earlier |
R1797 |
T7119 |
T7117 |
advmod |
earlier,starting |
R1798 |
T7120 |
T7119 |
prep |
than,earlier |
R1799 |
T7121 |
T7120 |
pobj |
stage,than |
R1800 |
T7122 |
T7121 |
nummod |
VII,stage |
R1801 |
T7123 |
T7117 |
cc |
and,starting |
R1802 |
T7124 |
T7117 |
conj |
extending,starting |
R1803 |
T7125 |
T7124 |
prep |
through,extending |
R1804 |
T7126 |
T7125 |
pobj |
stage,through |
R1805 |
T7127 |
T7126 |
nummod |
IX,stage |
R1806 |
T7128 |
T7129 |
punct |
(,data |
R1807 |
T7129 |
T7103 |
meta |
data,confirmed |
R1808 |
T7130 |
T7129 |
amod |
unpublished,data |
R1809 |
T7131 |
T7129 |
punct |
),data |
R1810 |
T7132 |
T7103 |
punct |
.,confirmed |
R1811 |
T7134 |
T7135 |
prep |
Within,concentrated |
R1812 |
T7136 |
T7137 |
compound |
pachytene,spermatocytes |
R1813 |
T7137 |
T7134 |
pobj |
spermatocytes,Within |
R1814 |
T7138 |
T7135 |
punct |
", ",concentrated |
R1815 |
T7139 |
T7135 |
nsubjpass |
DMRT7,concentrated |
R1816 |
T7140 |
T7135 |
auxpass |
is,concentrated |
R1817 |
T7141 |
T7135 |
prep |
in,concentrated |
R1818 |
T7142 |
T7143 |
det |
the,body |
R1819 |
T7143 |
T7141 |
pobj |
body,in |
R1820 |
T7144 |
T7143 |
compound |
XY,body |
R1821 |
T7145 |
T7143 |
punct |
", ",body |
R1822 |
T7146 |
T7143 |
cc |
or,body |
R1823 |
T7147 |
T7148 |
compound |
sex,body |
R1824 |
T7148 |
T7143 |
conj |
body,body |
R1825 |
T7149 |
T7143 |
punct |
", ",body |
R1826 |
T7150 |
T7151 |
det |
a,domain |
R1827 |
T7151 |
T7143 |
appos |
domain,body |
R1828 |
T7152 |
T7153 |
advmod |
densely,staining |
R1829 |
T7153 |
T7151 |
amod |
staining,domain |
R1830 |
T7154 |
T7151 |
compound |
chromatin,domain |
R1831 |
T7155 |
T7156 |
dep |
that,harbors |
R1832 |
T7156 |
T7151 |
relcl |
harbors,domain |
R1833 |
T7157 |
T7158 |
det |
the,chromosomes |
R1834 |
T7158 |
T7156 |
dobj |
chromosomes,harbors |
R1835 |
T7159 |
T7158 |
compound |
sex,chromosomes |
R1836 |
T7160 |
T7135 |
punct |
.,concentrated |
R1837 |
T7162 |
T7163 |
nsubj |
These,undergo |
R1838 |
T7164 |
T7165 |
amod |
transcriptional,inactivation |
R1839 |
T7165 |
T7163 |
dobj |
inactivation,undergo |
R1840 |
T7166 |
T7165 |
cc |
and,inactivation |
R1841 |
T7167 |
T7165 |
conj |
heterochromatinization,inactivation |
R1842 |
T7168 |
T7163 |
prep |
as,undergo |
R1843 |
T7169 |
T7170 |
det |
a,result |
R1844 |
T7170 |
T7168 |
pobj |
result,as |
R1845 |
T7171 |
T7170 |
prep |
of,result |
R1846 |
T7172 |
T7173 |
poss |
their,pairing |
R1847 |
T7173 |
T7171 |
pobj |
pairing,of |
R1848 |
T7174 |
T7173 |
amod |
incomplete,pairing |
R1849 |
T7175 |
T7173 |
prep |
during,pairing |
R1850 |
T7176 |
T7175 |
pobj |
prophase,during |
R1851 |
T7177 |
T7176 |
prep |
of,prophase |
R1852 |
T7178 |
T7179 |
amod |
mammalian,meiosis |
R1853 |
T7179 |
T7177 |
pobj |
meiosis,of |
R1854 |
T7180 |
T7179 |
amod |
male,meiosis |
R1855 |
T7181 |
T7182 |
punct |
[,17 |
R1856 |
T7182 |
T7163 |
parataxis |
17,undergo |
R1857 |
T7183 |
T7182 |
punct |
],17 |
R1858 |
T7184 |
T7163 |
punct |
.,undergo |
R1859 |
T7186 |
T7187 |
aux |
To,verify |
R1860 |
T7187 |
T7188 |
advcl |
verify,stained |
R1861 |
T7189 |
T7190 |
compound |
DMRT7,expression |
R1862 |
T7190 |
T7187 |
dobj |
expression,verify |
R1863 |
T7191 |
T7190 |
compound |
protein,expression |
R1864 |
T7192 |
T7187 |
prep |
in,verify |
R1865 |
T7193 |
T7194 |
det |
the,body |
R1866 |
T7194 |
T7192 |
pobj |
body,in |
R1867 |
T7195 |
T7194 |
compound |
XY,body |
R1868 |
T7196 |
T7188 |
punct |
", ",stained |
R1869 |
T7197 |
T7188 |
nsubj |
we,stained |
R1870 |
T7198 |
T7188 |
advmod |
double,stained |
R1871 |
T7199 |
T7188 |
punct |
-,stained |
R1872 |
T7200 |
T7201 |
compound |
mouse,testis |
R1873 |
T7201 |
T7202 |
compound |
testis,sections |
R1874 |
T7202 |
T7188 |
dobj |
sections,stained |
R1875 |
T7203 |
T7188 |
prep |
for,stained |
R1876 |
T7204 |
T7203 |
pobj |
DMRT7,for |
R1877 |
T7205 |
T7204 |
cc |
and,DMRT7 |
R1878 |
T7206 |
T7207 |
amod |
small,modifier |
R1879 |
T7207 |
T7204 |
conj |
modifier,DMRT7 |
R1880 |
T7208 |
T7209 |
npadvmod |
ubiquitin,related |
R1881 |
T7209 |
T7207 |
amod |
related,modifier |
R1882 |
T7210 |
T7209 |
punct |
-,related |
R1883 |
T7211 |
T7207 |
nummod |
1,modifier |
R1884 |
T7212 |
T7207 |
punct |
(,modifier |
R1885 |
T7213 |
T7207 |
appos |
SUMO,modifier |
R1886 |
T7214 |
T7213 |
punct |
-,SUMO |
R1887 |
T7215 |
T7213 |
nummod |
1,SUMO |
R1888 |
T7216 |
T7207 |
punct |
),modifier |
R1889 |
T7217 |
T7207 |
punct |
", ",modifier |
R1890 |
T7218 |
T7219 |
dep |
which,concentrated |
R1891 |
T7219 |
T7207 |
relcl |
concentrated,modifier |
R1892 |
T7220 |
T7219 |
auxpass |
is,concentrated |
R1893 |
T7221 |
T7219 |
prep |
in,concentrated |
R1894 |
T7222 |
T7223 |
det |
the,body |
R1895 |
T7223 |
T7221 |
pobj |
body,in |
R1896 |
T7224 |
T7223 |
compound |
XY,body |
R1897 |
T7225 |
T7219 |
prep |
during,concentrated |
R1898 |
T7226 |
T7225 |
pobj |
pachynema,during |
R1899 |
T7227 |
T7228 |
punct |
[,40 |
R1900 |
T7228 |
T7188 |
parataxis |
40,stained |
R1901 |
T7229 |
T7228 |
nummod |
39,40 |
R1902 |
T7230 |
T7228 |
punct |
",",40 |
R1903 |
T7231 |
T7228 |
punct |
],40 |
R1904 |
T7232 |
T7188 |
punct |
.,stained |
R1905 |
T7234 |
T7235 |
nsubjpass |
DMRT7,colocalized |
R1906 |
T7236 |
T7234 |
cc |
and,DMRT7 |
R1907 |
T7237 |
T7234 |
conj |
SUMO,DMRT7 |
R1908 |
T7238 |
T7237 |
punct |
-,SUMO |
R1909 |
T7239 |
T7237 |
nummod |
1,SUMO |
R1910 |
T7240 |
T7235 |
auxpass |
were,colocalized |
R1911 |
T7241 |
T7235 |
punct |
", ",colocalized |
R1912 |
T7242 |
T7235 |
advcl |
confirming,colocalized |
R1913 |
T7243 |
T7244 |
mark |
that,localized |
R1914 |
T7244 |
T7242 |
ccomp |
localized,confirming |
R1915 |
T7245 |
T7246 |
compound |
DMRT7,protein |
R1916 |
T7246 |
T7244 |
nsubjpass |
protein,localized |
R1917 |
T7247 |
T7244 |
auxpass |
is,localized |
R1918 |
T7248 |
T7244 |
advmod |
preferentially,localized |
R1919 |
T7249 |
T7244 |
prep |
to,localized |
R1920 |
T7250 |
T7251 |
det |
the,body |
R1921 |
T7251 |
T7249 |
pobj |
body,to |
R1922 |
T7252 |
T7251 |
compound |
XY,body |
R1923 |
T7253 |
T7254 |
punct |
(,1D |
R1924 |
T7254 |
T7235 |
parataxis |
1D,colocalized |
R1925 |
T7255 |
T7254 |
compound |
Figure,1D |
R1926 |
T7256 |
T7254 |
punct |
),1D |
R1927 |
T7257 |
T7235 |
punct |
.,colocalized |
R1928 |
T7259 |
T7260 |
nsubj |
We,confirmed |
R1929 |
T7261 |
T7260 |
advmod |
also,confirmed |
R1930 |
T7262 |
T7263 |
compound |
XY,localization |
R1931 |
T7263 |
T7260 |
dobj |
localization,confirmed |
R1932 |
T7264 |
T7263 |
compound |
body,localization |
R1933 |
T7265 |
T7263 |
prep |
of,localization |
R1934 |
T7266 |
T7265 |
pobj |
DMRT7,of |
R1935 |
T7267 |
T7260 |
prep |
by,confirmed |
R1936 |
T7268 |
T7269 |
amod |
double,staining |
R1937 |
T7269 |
T7267 |
pobj |
staining,by |
R1938 |
T7270 |
T7269 |
prep |
for,staining |
R1939 |
T7271 |
T7272 |
amod |
other,markers |
R1940 |
T7272 |
T7270 |
pobj |
markers,for |
R1941 |
T7273 |
T7272 |
prep |
including,markers |
R1942 |
T7274 |
T7275 |
compound |
Ub,H2A |
R1943 |
T7275 |
T7273 |
pobj |
H2A,including |
R1944 |
T7276 |
T7275 |
punct |
-,H2A |
R1945 |
T7277 |
T7275 |
cc |
and,H2A |
R1946 |
T7278 |
T7275 |
conj |
γH2AX,H2A |
R1947 |
T7279 |
T7280 |
punct |
(,data |
R1948 |
T7280 |
T7260 |
meta |
data,confirmed |
R1949 |
T7281 |
T7280 |
amod |
unpublished,data |
R1950 |
T7282 |
T7280 |
punct |
),data |
R1951 |
T7283 |
T7260 |
punct |
.,confirmed |
R1952 |
T7285 |
T7286 |
nsubjpass |
DMRT7,localized |
R1953 |
T7287 |
T7286 |
auxpass |
is,localized |
R1954 |
T7288 |
T7286 |
neg |
not,localized |
R1955 |
T7289 |
T7286 |
advmod |
preferentially,localized |
R1956 |
T7290 |
T7286 |
prep |
to,localized |
R1957 |
T7291 |
T7292 |
det |
the,body |
R1958 |
T7292 |
T7290 |
pobj |
body,to |
R1959 |
T7293 |
T7292 |
compound |
XY,body |
R1960 |
T7294 |
T7286 |
prep |
at,localized |
R1961 |
T7295 |
T7296 |
det |
all,stages |
R1962 |
T7296 |
T7294 |
pobj |
stages,at |
R1963 |
T7297 |
T7286 |
cc |
but,localized |
R1964 |
T7298 |
T7299 |
advmod |
instead,is |
R1965 |
T7299 |
T7286 |
conj |
is,localized |
R1966 |
T7300 |
T7299 |
acomp |
dynamic,is |
R1967 |
T7301 |
T7286 |
punct |
.,localized |
R1968 |
T7303 |
T7304 |
prep |
Based,appears |
R1969 |
T7305 |
T7303 |
prep |
on,Based |
R1970 |
T7306 |
T7307 |
amod |
epithelial,staging |
R1971 |
T7307 |
T7305 |
pobj |
staging,on |
R1972 |
T7308 |
T7304 |
punct |
", ",appears |
R1973 |
T7309 |
T7304 |
nsubj |
it,appears |
R1974 |
T7310 |
T7311 |
mark |
that,localizes |
R1975 |
T7311 |
T7304 |
ccomp |
localizes,appears |
R1976 |
T7312 |
T7311 |
nsubj |
DMRT7,localizes |
R1977 |
T7313 |
T7311 |
prep |
to,localizes |
R1978 |
T7314 |
T7315 |
det |
the,body |
R1979 |
T7315 |
T7313 |
pobj |
body,to |
R1980 |
T7316 |
T7315 |
compound |
XY,body |
R1981 |
T7317 |
T7311 |
prep |
from,localizes |
R1982 |
T7318 |
T7319 |
amod |
mid,pachynema |
R1983 |
T7319 |
T7317 |
pobj |
pachynema,from |
R1984 |
T7320 |
T7318 |
punct |
-,mid |
R1985 |
T7321 |
T7318 |
prep |
to,mid |
R1986 |
T7322 |
T7321 |
amod |
late,to |
R1987 |
T7323 |
T7319 |
punct |
-,pachynema |
R1988 |
T7324 |
T7311 |
punct |
", ",localizes |
R1989 |
T7325 |
T7311 |
conj |
becomes,localizes |
R1990 |
T7326 |
T7327 |
advmod |
diffusely,distributed |
R1991 |
T7327 |
T7325 |
acomp |
distributed,becomes |
R1992 |
T7328 |
T7325 |
prep |
in,becomes |
R1993 |
T7329 |
T7330 |
amod |
late,pachynema |
R1994 |
T7330 |
T7328 |
pobj |
pachynema,in |
R1995 |
T7331 |
T7330 |
punct |
-,pachynema |
R1996 |
T7332 |
T7325 |
punct |
", ",becomes |
R1997 |
T7333 |
T7325 |
cc |
and,becomes |
R1998 |
T7334 |
T7325 |
conj |
disappears,becomes |
R1999 |
T7335 |
T7334 |
prep |
in,disappears |
R2000 |
T7336 |
T7335 |
pobj |
diplonema,in |
R2001 |
T7337 |
T7338 |
punct |
(,data |
R2002 |
T7338 |
T7304 |
meta |
data,appears |
R2003 |
T7339 |
T7338 |
amod |
unpublished,data |
R2004 |
T7340 |
T7338 |
punct |
),data |
R2005 |
T7341 |
T7304 |
punct |
.,appears |
R2006 |
T7343 |
T7344 |
det |
This,localization |
R2007 |
T7344 |
T7345 |
nsubjpass |
localization,confirmed |
R2008 |
T7346 |
T7345 |
auxpass |
was,confirmed |
R2009 |
T7347 |
T7345 |
prep |
by,confirmed |
R2010 |
T7348 |
T7347 |
pobj |
staining,by |
R2011 |
T7349 |
T7348 |
prep |
of,staining |
R2012 |
T7350 |
T7351 |
amod |
meiotic,spreads |
R2013 |
T7351 |
T7349 |
pobj |
spreads,of |
R2014 |
T7352 |
T7353 |
punct |
(,S1 |
R2015 |
T7353 |
T7345 |
parataxis |
S1,confirmed |
R2016 |
T7354 |
T7353 |
compound |
Figure,S1 |
R2017 |
T7355 |
T7353 |
punct |
),S1 |
R2018 |
T7356 |
T7345 |
punct |
.,confirmed |
R2019 |
T7358 |
T7359 |
nsubjpass |
DMRT7,localized |
R2020 |
T7360 |
T7359 |
advmod |
also,localized |
R2021 |
T7361 |
T7359 |
auxpass |
is,localized |
R2022 |
T7362 |
T7359 |
advmod |
specifically,localized |
R2023 |
T7363 |
T7359 |
prep |
in,localized |
R2024 |
T7364 |
T7363 |
pobj |
sperm,in |
R2025 |
T7365 |
T7359 |
punct |
", ",localized |
R2026 |
T7366 |
T7359 |
prep |
with,localized |
R2027 |
T7367 |
T7368 |
compound |
antibody,staining |
R2028 |
T7368 |
T7366 |
pobj |
staining,with |
R2029 |
T7369 |
T7370 |
advmod |
mainly,in |
R2030 |
T7370 |
T7368 |
prep |
in,staining |
R2031 |
T7371 |
T7372 |
det |
the,ring |
R2032 |
T7372 |
T7370 |
pobj |
ring,in |
R2033 |
T7373 |
T7372 |
amod |
perinuclear,ring |
R2034 |
T7374 |
T7372 |
prep |
of,ring |
R2035 |
T7375 |
T7376 |
det |
the,manchette |
R2036 |
T7376 |
T7374 |
pobj |
manchette,of |
R2037 |
T7377 |
T7378 |
compound |
sperm,head |
R2038 |
T7378 |
T7376 |
compound |
head,manchette |
R2039 |
T7379 |
T7359 |
punct |
.,localized |
R2040 |
T7381 |
T7382 |
det |
This,staining |
R2041 |
T7382 |
T7383 |
nsubj |
staining,coincided |
R2042 |
T7384 |
T7383 |
prep |
with,coincided |
R2043 |
T7385 |
T7386 |
det |
the,stages |
R2044 |
T7386 |
T7384 |
pobj |
stages,with |
R2045 |
T7387 |
T7386 |
amod |
epithelial,stages |
R2046 |
T7388 |
T7389 |
prep |
in,localizes |
R2047 |
T7389 |
T7386 |
relcl |
localizes,stages |
R2048 |
T7390 |
T7388 |
pobj |
which,in |
R2049 |
T7391 |
T7389 |
nsubj |
DMRT7,localizes |
R2050 |
T7392 |
T7389 |
prep |
to,localizes |
R2051 |
T7393 |
T7394 |
det |
the,body |
R2052 |
T7394 |
T7392 |
pobj |
body,to |
R2053 |
T7395 |
T7394 |
compound |
XY,body |
R2054 |
T7396 |
T7394 |
prep |
in,body |
R2055 |
T7397 |
T7396 |
pobj |
spermatocytes,in |
R2056 |
T7398 |
T7399 |
punct |
(,1C |
R2057 |
T7399 |
T7383 |
parataxis |
1C,coincided |
R2058 |
T7400 |
T7399 |
compound |
Figure,1C |
R2059 |
T7401 |
T7399 |
cc |
and,1C |
R2060 |
T7402 |
T7399 |
conj |
1D,1C |
R2061 |
T7403 |
T7399 |
punct |
),1C |
R2062 |
T7404 |
T7383 |
punct |
.,coincided |
R1624 |
T6933 |
T6934 |
det |
the,wave |