PMC:1853120 / 47377-48011 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/1853120","sourcedb":"PMC","sourceid":"1853120","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1853120","text":"Histological analysis and TUNEL assay.\nDissected testes were fixed in Bouin's fixative or phosphate-buffered formalin overnight at 4 °C, progressively dehydrated in a graded ethanol series, and embedded in paraffin wax. Sections (6 μm) were deparaffinized, rehydrated, and stained with hematoxylin and eosin. For TUNEL analyses, deparaffinized sections were treated with proteinase K for 15 min and quenched in 3.0% hydrogen peroxide in PBS for 5 min at room temperature. 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