PMC:17827 / 47172-48405 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/17827","sourcedb":"PMC","sourceid":"17827","source_url":"http://www.ncbi.nlm.nih.gov/pmc/17827","text":"Negative isolation from primary culture\nNegative isolation of SFB from primary cultures of RASM using magnetobead-coupled anti-CD14 mAbs proved successful, providing satisfactory numbers of cells and avoiding repeated passaging and numerous cumulative population doublings. With culture times as short as 7 days, fresh SFB express surface and intracellular antigens typical of the FB lineage and/or SFB features as reported at a tissue level (Fig. 6 and Table 4) [1,2,12,13,14,15, 21,22,23,24,25,28]. This approach may also reduce the risk of in vitro growth selection. In the case of conventionally isolated RA-SFB, for example, the higher the number of passages, the higher the percentage of cells with trisomy 7 [33]. Some SFB functions (eg cell density at saturation and expression of transcription factors), in contrast, decrease at later passages [5,32,48].\nWhile the use of enzymes for the initial tissue digestion represents at least a transient stress for the cells, this method may also represent an alternative to tissue-outgrowth techniques, known to select for premitotic FB [26]. This is important since the relative contribution of pre-mitotic or postmitotic FB to the pathogenetic features of RA is presently unclear.","divisions":[{"label":"Title","span":{"begin":0,"end":39}}],"tracks":[{"project":"2_test","denotations":[{"id":"11178129-8912499-4386878","span":{"begin":464,"end":465},"obj":"8912499"},{"id":"11178129-7632096-4386879","span":{"begin":466,"end":467},"obj":"7632096"},{"id":"11178129-2596570-4386880","span":{"begin":468,"end":470},"obj":"2596570"},{"id":"11178129-2694400-4386881","span":{"begin":471,"end":473},"obj":"2694400"},{"id":"11178129-2084514-4386882","span":{"begin":474,"end":476},"obj":"2084514"},{"id":"11178129-8003048-4386883","span":{"begin":477,"end":479},"obj":"8003048"},{"id":"11178129-6366990-4386884","span":{"begin":481,"end":483},"obj":"6366990"},{"id":"11178129-2442194-4386885","span":{"begin":484,"end":486},"obj":"2442194"},{"id":"11178129-7510485-4386886","span":{"begin":490,"end":492},"obj":"7510485"},{"id":"11178129-1411083-4386887","span":{"begin":493,"end":495},"obj":"1411083"},{"id":"11178129-1657009-4386888","span":{"begin":496,"end":498},"obj":"1657009"},{"id":"11178129-8522321-4386889","span":{"begin":716,"end":718},"obj":"8522321"},{"id":"11178129-2784799-4386890","span":{"begin":854,"end":855},"obj":"2784799"},{"id":"11178129-13877250-4386891","span":{"begin":856,"end":858},"obj":"13877250"},{"id":"11178129-8207219-4386892","span":{"begin":859,"end":861},"obj":"8207219"},{"id":"11178129-2042650-4386893","span":{"begin":1089,"end":1091},"obj":"2042650"}],"attributes":[{"subj":"11178129-8912499-4386878","pred":"source","obj":"2_test"},{"subj":"11178129-7632096-4386879","pred":"source","obj":"2_test"},{"subj":"11178129-2596570-4386880","pred":"source","obj":"2_test"},{"subj":"11178129-2694400-4386881","pred":"source","obj":"2_test"},{"subj":"11178129-2084514-4386882","pred":"source","obj":"2_test"},{"subj":"11178129-8003048-4386883","pred":"source","obj":"2_test"},{"subj":"11178129-6366990-4386884","pred":"source","obj":"2_test"},{"subj":"11178129-2442194-4386885","pred":"source","obj":"2_test"},{"subj":"11178129-7510485-4386886","pred":"source","obj":"2_test"},{"subj":"11178129-1411083-4386887","pred":"source","obj":"2_test"},{"subj":"11178129-1657009-4386888","pred":"source","obj":"2_test"},{"subj":"11178129-8522321-4386889","pred":"source","obj":"2_test"},{"subj":"11178129-2784799-4386890","pred":"source","obj":"2_test"},{"subj":"11178129-13877250-4386891","pred":"source","obj":"2_test"},{"subj":"11178129-8207219-4386892","pred":"source","obj":"2_test"},{"subj":"11178129-2042650-4386893","pred":"source","obj":"2_test"}]},{"project":"Colil","denotations":[{"id":"T88","span":{"begin":854,"end":855},"obj":"2784799"},{"id":"T89","span":{"begin":856,"end":858},"obj":"13877250"},{"id":"T90","span":{"begin":859,"end":861},"obj":"8207219"},{"id":"T91","span":{"begin":1089,"end":1091},"obj":"2042650"},{"id":"T92","span":{"begin":464,"end":465},"obj":"8912499"},{"id":"T93","span":{"begin":466,"end":467},"obj":"7632096"},{"id":"T94","span":{"begin":468,"end":470},"obj":"2596570"},{"id":"T95","span":{"begin":471,"end":473},"obj":"2694400"},{"id":"T96","span":{"begin":474,"end":476},"obj":"2084514"},{"id":"T97","span":{"begin":477,"end":479},"obj":"8003048"},{"id":"T98","span":{"begin":481,"end":483},"obj":"6366990"},{"id":"T99","span":{"begin":484,"end":486},"obj":"2442194"},{"id":"T100","span":{"begin":490,"end":492},"obj":"7510485"},{"id":"T101","span":{"begin":493,"end":495},"obj":"1411083"},{"id":"T102","span":{"begin":496,"end":498},"obj":"1657009"},{"id":"T103","span":{"begin":716,"end":718},"obj":"8522321"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/"}],"attributes":[{"subj":"T88","pred":"source","obj":"Colil"},{"subj":"T89","pred":"source","obj":"Colil"},{"subj":"T90","pred":"source","obj":"Colil"},{"subj":"T91","pred":"source","obj":"Colil"},{"subj":"T92","pred":"source","obj":"Colil"},{"subj":"T93","pred":"source","obj":"Colil"},{"subj":"T94","pred":"source","obj":"Colil"},{"subj":"T95","pred":"source","obj":"Colil"},{"subj":"T96","pred":"source","obj":"Colil"},{"subj":"T97","pred":"source","obj":"Colil"},{"subj":"T98","pred":"source","obj":"Colil"},{"subj":"T99","pred":"source","obj":"Colil"},{"subj":"T100","pred":"source","obj":"Colil"},{"subj":"T101","pred":"source","obj":"Colil"},{"subj":"T102","pred":"source","obj":"Colil"},{"subj":"T103","pred":"source","obj":"Colil"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#9396ec","default":true},{"id":"Colil","color":"#b0ec93"}]}]}}