PMC:1564426 / 20242-20927 JSONTXT

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T9575","span":{"begin":1,"end":4},"obj":"PR_EXT:000004122"},{"id":"T9576","span":{"begin":5,"end":16},"obj":"SO_EXT:0000673"},{"id":"T9577","span":{"begin":39,"end":41},"obj":"GO_EXT:reverse_transcription_or_reverse_transcriptase"},{"id":"T9578","span":{"begin":51,"end":58},"obj":"SO_EXT:0000112"},{"id":"T9579","span":{"begin":68,"end":72},"obj":"SO_EXT:0000147"},{"id":"T9580","span":{"begin":100,"end":103},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T9581","span":{"begin":136,"end":142},"obj":"UBERON:0000479"},{"id":"T9582","span":{"begin":177,"end":179},"obj":"GO_EXT:reverse_transcription_or_reverse_transcriptase"},{"id":"T9583","span":{"begin":180,"end":191},"obj":"SO_EXT:0000006"},{"id":"T9584","span":{"begin":197,"end":199},"obj":"SO_EXT:0000028"},{"id":"T9585","span":{"begin":210,"end":219},"obj":"SO_EXT:sequence_truncation_process"},{"id":"T9586","span":{"begin":220,"end":223},"obj":"PR_EXT:000004122"},{"id":"T9587","span":{"begin":225,"end":228},"obj":"PR_EXT:000004122"},{"id":"T9588","span":{"begin":234,"end":240},"obj":"SO_EXT:0001023"},{"id":"T9589","span":{"begin":253,"end":260},"obj":"UBERON:0000479"},{"id":"T9590","span":{"begin":271,"end":282},"obj":"GO_EXT:recombinase"},{"id":"T9591","span":{"begin":298,"end":307},"obj":"GO:0010467"},{"id":"T9592","span":{"begin":315,"end":318},"obj":"PR_EXT:000009454"},{"id":"T9593","span":{"begin":332,"end":336},"obj":"NCBITaxon:10088"},{"id":"T9594","span":{"begin":391,"end":394},"obj":"PR_EXT:000009454"},{"id":"T9595","span":{"begin":408,"end":413},"obj":"NCBITaxon:10088"},{"id":"T9596","span":{"begin":414,"end":421},"obj":"UBERON:0000479"},{"id":"T9597","span":{"begin":437,"end":442},"obj":"UBERON:0002107"},{"id":"T9598","span":{"begin":446,"end":449},"obj":"PR_EXT:000009454"},{"id":"T9599","span":{"begin":463,"end":467},"obj":"NCBITaxon:10088"},{"id":"T9600","span":{"begin":499,"end":508},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T9601","span":{"begin":509,"end":515},"obj":"SO_EXT:0001023"},{"id":"T9602","span":{"begin":521,"end":523},"obj":"SO_EXT:0000028"},{"id":"T9603","span":{"begin":549,"end":552},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T9604","span":{"begin":599,"end":612},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T9605","span":{"begin":636,"end":642},"obj":"UBERON:0000479"},{"id":"T9606","span":{"begin":648,"end":656},"obj":"SO_EXT:genotype_or_entity_with_genotype"}],"text":" Apc transcripts were also analyzed by RT-PCR with primers spanning exon 14 (Figure 1A) using total RNA isolated from the corresponding tissue samples. We detected the expected RT-PCR product (313 bp) from the truncated Apc (ApcΔ580) allele only in the tissues where Cre recombinase is known to be expressed in the K14-cre–positive mice. However, this product was not detected in either the K14-cre–negative mouse tissues samples or the liver of K14-cre–positive mice, and only the product from the wild-type allele (528 bp) was detected from these RNA samples, further confirming that Cre-mediated recombination has taken place in the tissue- and genotype-specific manner (Figure 3B)."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T9493","span":{"begin":1,"end":4},"obj":"PR:000004122"},{"id":"T9494","span":{"begin":5,"end":16},"obj":"SO:0000673"},{"id":"T9495","span":{"begin":51,"end":58},"obj":"SO:0000112"},{"id":"T9496","span":{"begin":68,"end":72},"obj":"SO:0000147"},{"id":"T9497","span":{"begin":136,"end":142},"obj":"UBERON:0000479"},{"id":"T9498","span":{"begin":180,"end":191},"obj":"SO:0000006"},{"id":"T9499","span":{"begin":197,"end":199},"obj":"SO:0000028"},{"id":"T9500","span":{"begin":220,"end":223},"obj":"PR:000004122"},{"id":"T9501","span":{"begin":225,"end":228},"obj":"PR:000004122"},{"id":"T9502","span":{"begin":234,"end":240},"obj":"SO:0001023"},{"id":"T9503","span":{"begin":253,"end":260},"obj":"UBERON:0000479"},{"id":"T9504","span":{"begin":298,"end":307},"obj":"GO:0010467"},{"id":"T9505","span":{"begin":315,"end":318},"obj":"PR:000009454"},{"id":"T9506","span":{"begin":332,"end":336},"obj":"NCBITaxon:10088"},{"id":"T9507","span":{"begin":391,"end":394},"obj":"PR:000009454"},{"id":"T9508","span":{"begin":408,"end":413},"obj":"NCBITaxon:10088"},{"id":"T9509","span":{"begin":414,"end":421},"obj":"UBERON:0000479"},{"id":"T9510","span":{"begin":437,"end":442},"obj":"UBERON:0002107"},{"id":"T9511","span":{"begin":446,"end":449},"obj":"PR:000009454"},{"id":"T9512","span":{"begin":463,"end":467},"obj":"NCBITaxon:10088"},{"id":"T9513","span":{"begin":509,"end":515},"obj":"SO:0001023"},{"id":"T9514","span":{"begin":521,"end":523},"obj":"SO:0000028"},{"id":"T9515","span":{"begin":636,"end":642},"obj":"UBERON:0000479"}],"text":" Apc transcripts were also analyzed by RT-PCR with primers spanning exon 14 (Figure 1A) using total RNA isolated from the corresponding tissue samples. We detected the expected RT-PCR product (313 bp) from the truncated Apc (ApcΔ580) allele only in the tissues where Cre recombinase is known to be expressed in the K14-cre–positive mice. However, this product was not detected in either the K14-cre–negative mouse tissues samples or the liver of K14-cre–positive mice, and only the product from the wild-type allele (528 bp) was detected from these RNA samples, further confirming that Cre-mediated recombination has taken place in the tissue- and genotype-specific manner (Figure 3B)."}

{"project":"craft-sa-dev","denotations":[{"id":"T9831","span":{"begin":1,"end":4},"obj":"NN"},{"id":"T9832","span":{"begin":5,"end":16},"obj":"NNS"},{"id":"T9833","span":{"begin":27,"end":35},"obj":"VBN"},{"id":"T9834","span":{"begin":17,"end":21},"obj":"VBD"},{"id":"T9835","span":{"begin":22,"end":26},"obj":"RB"},{"id":"T9836","span":{"begin":36,"end":38},"obj":"IN"},{"id":"T9837","span":{"begin":39,"end":41},"obj":"NN"},{"id":"T9838","span":{"begin":42,"end":45},"obj":"NN"},{"id":"T9839","span":{"begin":41,"end":42},"obj":"HYPH"},{"id":"T9840","span":{"begin":46,"end":50},"obj":"IN"},{"id":"T9841","span":{"begin":51,"end":58},"obj":"NNS"},{"id":"T9842","span":{"begin":59,"end":67},"obj":"VBG"},{"id":"T9843","span":{"begin":68,"end":72},"obj":"NN"},{"id":"T9844","span":{"begin":73,"end":75},"obj":"CD"},{"id":"T9845","span":{"begin":76,"end":77},"obj":"-LRB-"},{"id":"T9846","span":{"begin":84,"end":86},"obj":"NN"},{"id":"T9847","span":{"begin":77,"end":83},"obj":"NN"},{"id":"T9848","span":{"begin":86,"end":87},"obj":"-RRB-"},{"id":"T9849","span":{"begin":88,"end":93},"obj":"VBG"},{"id":"T9850","span":{"begin":94,"end":99},"obj":"JJ"},{"id":"T9851","span":{"begin":100,"end":103},"obj":"NN"},{"id":"T9852","span":{"begin":104,"end":112},"obj":"VBN"},{"id":"T9853","span":{"begin":113,"end":117},"obj":"IN"},{"id":"T9854","span":{"begin":118,"end":121},"obj":"DT"},{"id":"T9855","span":{"begin":143,"end":150},"obj":"NNS"},{"id":"T9856","span":{"begin":122,"end":135},"obj":"VBG"},{"id":"T9857","span":{"begin":136,"end":142},"obj":"NN"},{"id":"T9858","span":{"begin":150,"end":151},"obj":"."},{"id":"T9859","span":{"begin":151,"end":337},"obj":"sentence"},{"id":"T9860","span":{"begin":152,"end":154},"obj":"PRP"},{"id":"T9861","span":{"begin":155,"end":163},"obj":"VBD"},{"id":"T9862","span":{"begin":164,"end":167},"obj":"DT"},{"id":"T9863","span":{"begin":184,"end":191},"obj":"NN"},{"id":"T9864","span":{"begin":168,"end":176},"obj":"VBN"},{"id":"T9865","span":{"begin":177,"end":179},"obj":"NN"},{"id":"T9866","span":{"begin":180,"end":183},"obj":"NN"},{"id":"T9867","span":{"begin":179,"end":180},"obj":"HYPH"},{"id":"T9868","span":{"begin":192,"end":193},"obj":"-LRB-"},{"id":"T9869","span":{"begin":197,"end":199},"obj":"NN"},{"id":"T9870","span":{"begin":193,"end":196},"obj":"CD"},{"id":"T9871","span":{"begin":199,"end":200},"obj":"-RRB-"},{"id":"T9872","span":{"begin":201,"end":205},"obj":"IN"},{"id":"T9873","span":{"begin":206,"end":209},"obj":"DT"},{"id":"T9874","span":{"begin":234,"end":240},"obj":"NN"},{"id":"T9875","span":{"begin":210,"end":219},"obj":"VBN"},{"id":"T9876","span":{"begin":220,"end":223},"obj":"NN"},{"id":"T9877","span":{"begin":224,"end":225},"obj":"-LRB-"},{"id":"T9878","span":{"begin":225,"end":232},"obj":"NN"},{"id":"T9879","span":{"begin":232,"end":233},"obj":"-RRB-"},{"id":"T9880","span":{"begin":241,"end":245},"obj":"RB"},{"id":"T9881","span":{"begin":246,"end":248},"obj":"IN"},{"id":"T9882","span":{"begin":249,"end":252},"obj":"DT"},{"id":"T9883","span":{"begin":253,"end":260},"obj":"NNS"},{"id":"T9884","span":{"begin":261,"end":266},"obj":"WRB"},{"id":"T9885","span":{"begin":286,"end":291},"obj":"VBN"},{"id":"T9886","span":{"begin":267,"end":270},"obj":"NN"},{"id":"T9887","span":{"begin":271,"end":282},"obj":"NN"},{"id":"T9888","span":{"begin":283,"end":285},"obj":"VBZ"},{"id":"T9889","span":{"begin":292,"end":294},"obj":"TO"},{"id":"T9890","span":{"begin":298,"end":307},"obj":"VBN"},{"id":"T9891","span":{"begin":295,"end":297},"obj":"VB"},{"id":"T9892","span":{"begin":308,"end":310},"obj":"IN"},{"id":"T9893","span":{"begin":311,"end":314},"obj":"DT"},{"id":"T9894","span":{"begin":332,"end":336},"obj":"NNS"},{"id":"T9895","span":{"begin":315,"end":318},"obj":"NN"},{"id":"T9896","span":{"begin":319,"end":322},"obj":"NN"},{"id":"T9897","span":{"begin":318,"end":319},"obj":"HYPH"},{"id":"T9898","span":{"begin":323,"end":331},"obj":"JJ"},{"id":"T9899","span":{"begin":322,"end":323},"obj":"HYPH"},{"id":"T9900","span":{"begin":336,"end":337},"obj":"."},{"id":"T9901","span":{"begin":337,"end":685},"obj":"sentence"},{"id":"T9902","span":{"begin":338,"end":345},"obj":"RB"},{"id":"T9903","span":{"begin":368,"end":376},"obj":"VBN"},{"id":"T9904","span":{"begin":345,"end":347},"obj":","},{"id":"T9905","span":{"begin":347,"end":351},"obj":"DT"},{"id":"T9906","span":{"begin":352,"end":359},"obj":"NN"},{"id":"T9907","span":{"begin":360,"end":363},"obj":"VBD"},{"id":"T9908","span":{"begin":364,"end":367},"obj":"RB"},{"id":"T9909","span":{"begin":377,"end":379},"obj":"IN"},{"id":"T9910","span":{"begin":380,"end":386},"obj":"CC"},{"id":"T9911","span":{"begin":422,"end":429},"obj":"NNS"},{"id":"T9912","span":{"begin":387,"end":390},"obj":"DT"},{"id":"T9913","span":{"begin":391,"end":394},"obj":"NN"},{"id":"T9914","span":{"begin":395,"end":398},"obj":"NN"},{"id":"T9915","span":{"begin":394,"end":395},"obj":"HYPH"},{"id":"T9916","span":{"begin":399,"end":407},"obj":"JJ"},{"id":"T9917","span":{"begin":398,"end":399},"obj":"HYPH"},{"id":"T9918","span":{"begin":408,"end":413},"obj":"NN"},{"id":"T9919","span":{"begin":414,"end":421},"obj":"NNS"},{"id":"T9920","span":{"begin":430,"end":432},"obj":"CC"},{"id":"T9921","span":{"begin":433,"end":436},"obj":"DT"},{"id":"T9922","span":{"begin":437,"end":442},"obj":"NN"},{"id":"T9923","span":{"begin":443,"end":445},"obj":"IN"},{"id":"T9924","span":{"begin":446,"end":449},"obj":"NN"},{"id":"T9925","span":{"begin":450,"end":453},"obj":"NN"},{"id":"T9926","span":{"begin":449,"end":450},"obj":"HYPH"},{"id":"T9927","span":{"begin":454,"end":462},"obj":"JJ"},{"id":"T9928","span":{"begin":453,"end":454},"obj":"HYPH"},{"id":"T9929","span":{"begin":463,"end":467},"obj":"NNS"},{"id":"T9930","span":{"begin":467,"end":469},"obj":","},{"id":"T9931","span":{"begin":469,"end":472},"obj":"CC"},{"id":"T9932","span":{"begin":473,"end":477},"obj":"RB"},{"id":"T9933","span":{"begin":482,"end":489},"obj":"NN"},{"id":"T9934","span":{"begin":478,"end":481},"obj":"DT"},{"id":"T9935","span":{"begin":529,"end":537},"obj":"VBN"},{"id":"T9936","span":{"begin":490,"end":494},"obj":"IN"},{"id":"T9937","span":{"begin":495,"end":498},"obj":"DT"},{"id":"T9938","span":{"begin":509,"end":515},"obj":"NN"},{"id":"T9939","span":{"begin":499,"end":503},"obj":"JJ"},{"id":"T9940","span":{"begin":504,"end":508},"obj":"NN"},{"id":"T9941","span":{"begin":503,"end":504},"obj":"HYPH"},{"id":"T9942","span":{"begin":516,"end":517},"obj":"-LRB-"},{"id":"T9943","span":{"begin":521,"end":523},"obj":"NN"},{"id":"T9944","span":{"begin":517,"end":520},"obj":"CD"},{"id":"T9945","span":{"begin":523,"end":524},"obj":"-RRB-"},{"id":"T9946","span":{"begin":525,"end":528},"obj":"VBD"},{"id":"T9947","span":{"begin":538,"end":542},"obj":"IN"},{"id":"T9948","span":{"begin":543,"end":548},"obj":"DT"},{"id":"T9949","span":{"begin":553,"end":560},"obj":"NNS"},{"id":"T9950","span":{"begin":549,"end":552},"obj":"NN"},{"id":"T9951","span":{"begin":560,"end":562},"obj":","},{"id":"T9952","span":{"begin":562,"end":569},"obj":"RB"},{"id":"T9953","span":{"begin":570,"end":580},"obj":"VBG"},{"id":"T9954","span":{"begin":581,"end":585},"obj":"IN"},{"id":"T9955","span":{"begin":617,"end":622},"obj":"VBN"},{"id":"T9956","span":{"begin":586,"end":589},"obj":"NN"},{"id":"T9957","span":{"begin":590,"end":598},"obj":"JJ"},{"id":"T9958","span":{"begin":589,"end":590},"obj":"HYPH"},{"id":"T9959","span":{"begin":599,"end":612},"obj":"NN"},{"id":"T9960","span":{"begin":613,"end":616},"obj":"VBZ"},{"id":"T9961","span":{"begin":623,"end":628},"obj":"NN"},{"id":"T9962","span":{"begin":629,"end":631},"obj":"IN"},{"id":"T9963","span":{"begin":632,"end":635},"obj":"DT"},{"id":"T9964","span":{"begin":666,"end":672},"obj":"NN"},{"id":"T9965","span":{"begin":636,"end":642},"obj":"NN"},{"id":"T9966","span":{"begin":657,"end":665},"obj":"JJ"},{"id":"T9967","span":{"begin":642,"end":643},"obj":"HYPH"},{"id":"T9968","span":{"begin":644,"end":647},"obj":"CC"},{"id":"T9969","span":{"begin":648,"end":656},"obj":"NN"},{"id":"T9970","span":{"begin":656,"end":657},"obj":"HYPH"},{"id":"T9971","span":{"begin":673,"end":674},"obj":"-LRB-"},{"id":"T9972","span":{"begin":681,"end":683},"obj":"NN"},{"id":"T9973","span":{"begin":674,"end":680},"obj":"NN"},{"id":"T9974","span":{"begin":683,"end":684},"obj":"-RRB-"},{"id":"T9975","span":{"begin":684,"end":685},"obj":"."}],"relations":[{"id":"R2893","pred":"compound","subj":"T9831","obj":"T9832"},{"id":"R2894","pred":"nsubjpass","subj":"T9832","obj":"T9833"},{"id":"R2895","pred":"auxpass","subj":"T9834","obj":"T9833"},{"id":"R2896","pred":"advmod","subj":"T9835","obj":"T9833"},{"id":"R2897","pred":"prep","subj":"T9836","obj":"T9833"},{"id":"R2898","pred":"compound","subj":"T9837","obj":"T9838"},{"id":"R2899","pred":"pobj","subj":"T9838","obj":"T9836"},{"id":"R2900","pred":"punct","subj":"T9839","obj":"T9838"},{"id":"R2901","pred":"prep","subj":"T9840","obj":"T9838"},{"id":"R2902","pred":"pobj","subj":"T9841","obj":"T9840"},{"id":"R2903","pred":"acl","subj":"T9842","obj":"T9841"},{"id":"R2904","pred":"dobj","subj":"T9843","obj":"T9842"},{"id":"R2905","pred":"nummod","subj":"T9844","obj":"T9843"},{"id":"R2906","pred":"punct","subj":"T9845","obj":"T9846"},{"id":"R2907","pred":"parataxis","subj":"T9846","obj":"T9833"},{"id":"R2908","pred":"compound","subj":"T9847","obj":"T9846"},{"id":"R2909","pred":"punct","subj":"T9848","obj":"T9846"},{"id":"R2910","pred":"advcl","subj":"T9849","obj":"T9833"},{"id":"R2911","pred":"amod","subj":"T9850","obj":"T9851"},{"id":"R2912","pred":"dobj","subj":"T9851","obj":"T9849"},{"id":"R2913","pred":"acl","subj":"T9852","obj":"T9851"},{"id":"R2914","pred":"prep","subj":"T9853","obj":"T9852"},{"id":"R2915","pred":"det","subj":"T9854","obj":"T9855"},{"id":"R2916","pred":"pobj","subj":"T9855","obj":"T9853"},{"id":"R2917","pred":"amod","subj":"T9856","obj":"T9855"},{"id":"R2918","pred":"compound","subj":"T9857","obj":"T9855"},{"id":"R2919","pred":"punct","subj":"T9858","obj":"T9833"},{"id":"R2920","pred":"nsubj","subj":"T9860","obj":"T9861"},{"id":"R2921","pred":"det","subj":"T9862","obj":"T9863"},{"id":"R2922","pred":"dobj","subj":"T9863","obj":"T9861"},{"id":"R2923","pred":"amod","subj":"T9864","obj":"T9863"},{"id":"R2924","pred":"compound","subj":"T9865","obj":"T9866"},{"id":"R2925","pred":"compound","subj":"T9866","obj":"T9863"},{"id":"R2926","pred":"punct","subj":"T9867","obj":"T9866"},{"id":"R2927","pred":"punct","subj":"T9868","obj":"T9869"},{"id":"R2928","pred":"parataxis","subj":"T9869","obj":"T9863"},{"id":"R2929","pred":"nummod","subj":"T9870","obj":"T9869"},{"id":"R2930","pred":"punct","subj":"T9871","obj":"T9869"},{"id":"R2931","pred":"prep","subj":"T9872","obj":"T9863"},{"id":"R2932","pred":"det","subj":"T9873","obj":"T9874"},{"id":"R2933","pred":"pobj","subj":"T9874","obj":"T9872"},{"id":"R2934","pred":"amod","subj":"T9875","obj":"T9876"},{"id":"R2935","pred":"nmod","subj":"T9876","obj":"T9874"},{"id":"R2936","pred":"punct","subj":"T9877","obj":"T9876"},{"id":"R2937","pred":"appos","subj":"T9878","obj":"T9876"},{"id":"R2938","pred":"punct","subj":"T9879","obj":"T9874"},{"id":"R2939","pred":"advmod","subj":"T9880","obj":"T9881"},{"id":"R2940","pred":"prep","subj":"T9881","obj":"T9861"},{"id":"R2941","pred":"det","subj":"T9882","obj":"T9883"},{"id":"R2942","pred":"pobj","subj":"T9883","obj":"T9881"},{"id":"R2943","pred":"advmod","subj":"T9884","obj":"T9885"},{"id":"R2944","pred":"advcl","subj":"T9885","obj":"T9883"},{"id":"R2945","pred":"compound","subj":"T9886","obj":"T9887"},{"id":"R2946","pred":"nsubjpass","subj":"T9887","obj":"T9885"},{"id":"R2947","pred":"auxpass","subj":"T9888","obj":"T9885"},{"id":"R2948","pred":"aux","subj":"T9889","obj":"T9890"},{"id":"R2949","pred":"xcomp","subj":"T9890","obj":"T9885"},{"id":"R2950","pred":"auxpass","subj":"T9891","obj":"T9890"},{"id":"R2951","pred":"prep","subj":"T9892","obj":"T9890"},{"id":"R2952","pred":"det","subj":"T9893","obj":"T9894"},{"id":"R2953","pred":"pobj","subj":"T9894","obj":"T9892"},{"id":"R2954","pred":"compound","subj":"T9895","obj":"T9896"},{"id":"R2955","pred":"npadvmod","subj":"T9896","obj":"T9898"},{"id":"R2956","pred":"punct","subj":"T9897","obj":"T9896"},{"id":"R2957","pred":"amod","subj":"T9898","obj":"T9894"},{"id":"R2958","pred":"punct","subj":"T9899","obj":"T9898"},{"id":"R2959","pred":"punct","subj":"T9900","obj":"T9861"},{"id":"R2960","pred":"advmod","subj":"T9902","obj":"T9903"},{"id":"R2961","pred":"punct","subj":"T9904","obj":"T9903"},{"id":"R2962","pred":"det","subj":"T9905","obj":"T9906"},{"id":"R2963","pred":"nsubjpass","subj":"T9906","obj":"T9903"},{"id":"R2964","pred":"auxpass","subj":"T9907","obj":"T9903"},{"id":"R2965","pred":"neg","subj":"T9908","obj":"T9903"},{"id":"R2966","pred":"prep","subj":"T9909","obj":"T9903"},{"id":"R2967","pred":"preconj","subj":"T9910","obj":"T9911"},{"id":"R2968","pred":"pobj","subj":"T9911","obj":"T9909"},{"id":"R2969","pred":"det","subj":"T9912","obj":"T9911"},{"id":"R2970","pred":"compound","subj":"T9913","obj":"T9914"},{"id":"R2971","pred":"npadvmod","subj":"T9914","obj":"T9916"},{"id":"R2972","pred":"punct","subj":"T9915","obj":"T9914"},{"id":"R2973","pred":"amod","subj":"T9916","obj":"T9911"},{"id":"R2974","pred":"punct","subj":"T9917","obj":"T9916"},{"id":"R2975","pred":"compound","subj":"T9918","obj":"T9919"},{"id":"R2976","pred":"compound","subj":"T9919","obj":"T9911"},{"id":"R2977","pred":"cc","subj":"T9920","obj":"T9911"},{"id":"R2978","pred":"det","subj":"T9921","obj":"T9922"},{"id":"R2979","pred":"conj","subj":"T9922","obj":"T9911"},{"id":"R2980","pred":"prep","subj":"T9923","obj":"T9922"},{"id":"R2981","pred":"compound","subj":"T9924","obj":"T9925"},{"id":"R2982","pred":"npadvmod","subj":"T9925","obj":"T9927"},{"id":"R2983","pred":"punct","subj":"T9926","obj":"T9925"},{"id":"R2984","pred":"amod","subj":"T9927","obj":"T9929"},{"id":"R2985","pred":"punct","subj":"T9928","obj":"T9927"},{"id":"R2986","pred":"pobj","subj":"T9929","obj":"T9923"},{"id":"R2987","pred":"punct","subj":"T9930","obj":"T9903"},{"id":"R2988","pred":"cc","subj":"T9931","obj":"T9903"},{"id":"R2989","pred":"advmod","subj":"T9932","obj":"T9933"},{"id":"R2990","pred":"nsubjpass","subj":"T9933","obj":"T9935"},{"id":"R2991","pred":"det","subj":"T9934","obj":"T9933"},{"id":"R2992","pred":"conj","subj":"T9935","obj":"T9903"},{"id":"R2993","pred":"prep","subj":"T9936","obj":"T9933"},{"id":"R2994","pred":"det","subj":"T9937","obj":"T9938"},{"id":"R2995","pred":"pobj","subj":"T9938","obj":"T9936"},{"id":"R2996","pred":"amod","subj":"T9939","obj":"T9940"},{"id":"R2997","pred":"compound","subj":"T9940","obj":"T9938"},{"id":"R2998","pred":"punct","subj":"T9941","obj":"T9940"},{"id":"R2999","pred":"punct","subj":"T9942","obj":"T9943"},{"id":"R3000","pred":"parataxis","subj":"T9943","obj":"T9933"},{"id":"R3001","pred":"nummod","subj":"T9944","obj":"T9943"},{"id":"R3002","pred":"punct","subj":"T9945","obj":"T9943"},{"id":"R3003","pred":"auxpass","subj":"T9946","obj":"T9935"},{"id":"R3004","pred":"prep","subj":"T9947","obj":"T9935"},{"id":"R3005","pred":"det","subj":"T9948","obj":"T9949"},{"id":"R3006","pred":"pobj","subj":"T9949","obj":"T9947"},{"id":"R3007","pred":"compound","subj":"T9950","obj":"T9949"},{"id":"R3008","pred":"punct","subj":"T9951","obj":"T9935"},{"id":"R3009","pred":"advmod","subj":"T9952","obj":"T9953"},{"id":"R3010","pred":"advcl","subj":"T9953","obj":"T9935"},{"id":"R3011","pred":"mark","subj":"T9954","obj":"T9955"},{"id":"R3012","pred":"ccomp","subj":"T9955","obj":"T9953"},{"id":"R3013","pred":"npadvmod","subj":"T9956","obj":"T9957"},{"id":"R3014","pred":"amod","subj":"T9957","obj":"T9959"},{"id":"R3015","pred":"punct","subj":"T9958","obj":"T9957"},{"id":"R3016","pred":"nsubj","subj":"T9959","obj":"T9955"},{"id":"R3017","pred":"aux","subj":"T9960","obj":"T9955"},{"id":"R3018","pred":"dobj","subj":"T9961","obj":"T9955"},{"id":"R3019","pred":"prep","subj":"T9962","obj":"T9955"},{"id":"R3020","pred":"det","subj":"T9963","obj":"T9964"},{"id":"R3021","pred":"pobj","subj":"T9964","obj":"T9962"},{"id":"R3022","pred":"npadvmod","subj":"T9965","obj":"T9966"},{"id":"R3023","pred":"amod","subj":"T9966","obj":"T9964"},{"id":"R3024","pred":"punct","subj":"T9967","obj":"T9965"},{"id":"R3025","pred":"cc","subj":"T9968","obj":"T9965"},{"id":"R3026","pred":"conj","subj":"T9969","obj":"T9965"},{"id":"R3027","pred":"punct","subj":"T9970","obj":"T9966"},{"id":"R3028","pred":"punct","subj":"T9971","obj":"T9972"},{"id":"R3029","pred":"parataxis","subj":"T9972","obj":"T9935"},{"id":"R3030","pred":"compound","subj":"T9973","obj":"T9972"},{"id":"R3031","pred":"punct","subj":"T9974","obj":"T9972"},{"id":"R3032","pred":"punct","subj":"T9975","obj":"T9903"}],"text":" Apc transcripts were also analyzed by RT-PCR with primers spanning exon 14 (Figure 1A) using total RNA isolated from the corresponding tissue samples. We detected the expected RT-PCR product (313 bp) from the truncated Apc (ApcΔ580) allele only in the tissues where Cre recombinase is known to be expressed in the K14-cre–positive mice. However, this product was not detected in either the K14-cre–negative mouse tissues samples or the liver of K14-cre–positive mice, and only the product from the wild-type allele (528 bp) was detected from these RNA samples, further confirming that Cre-mediated recombination has taken place in the tissue- and genotype-specific manner (Figure 3B)."}