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PMC:1540739 / 8943-10206 JSONTXT

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Id Subject Object Predicate Lexical cue
T5764 0-2 TO denotes To
T5765 3-7 VB denotes make
T5766 43-52 VBD denotes subcloned
T5767 8-9 DT denotes a
T5768 25-32 NN denotes protein
T5769 10-13 NN denotes p53
T5770 14-17 NN denotes GFP
T5771 13-14 HYPH denotes -
T5772 18-24 NN denotes fusion
T5773 32-34 , denotes ,
T5774 34-36 PRP denotes we
T5775 37-42 RB denotes first
T5776 53-54 DT denotes a
T5777 69-77 NN denotes fragment
T5778 55-60 NN denotes SacII
T5779 61-68 NN denotes HindIII
T5780 60-61 HYPH denotes -
T5781 78-80 IN denotes of
T5782 81-84 DT denotes the
T5783 89-94 NN denotes locus
T5784 85-88 NN denotes p53
T5785 95-96 -LRB- denotes (
T5786 96-109 VBG denotes corresponding
T5787 110-112 IN denotes to
T5788 113-117 NN denotes part
T5789 118-120 IN denotes of
T5790 121-125 NN denotes exon
T5791 126-128 CD denotes 10
T5792 129-131 IN denotes to
T5793 132-141 NNS denotes sequences
T5794 142-152 RB denotes downstream
T5795 153-155 IN denotes of
T5796 156-159 DT denotes the
T5797 160-164 NN denotes gene
T5798 164-165 -RRB- denotes )
T5799 166-170 IN denotes into
T5800 171-174 NN denotes pBS
T5801 174-176 , denotes ,
T5802 176-180 RB denotes then
T5803 181-188 VBD denotes mutated
T5804 189-192 DT denotes the
T5805 201-205 NN denotes site
T5806 193-200 NN denotes HindIII
T5807 206-210 IN denotes into
T5808 211-212 DT denotes a
T5809 218-222 NN denotes site
T5810 213-217 NN denotes FseI
T5811 222-223 . denotes .
T5812 223-614 sentence denotes We next mutated the C-terminal part of the p53 gene in two rounds of PCR mutagenesis, first with primers 5′-GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC-3′ and 5′-GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC-3′, which removed the stop codon and introduced a BamHI site, then with primers 5′-GACGGATCCCCTCTGAATTCCGTCCCCATCACCA-3′ and 5′-TGGTGATGGGGACGGAATACAGAGGGGATCCGTC-3′, which introduced an EcoRI site.
T5813 224-226 PRP denotes We
T5814 232-239 VBD denotes mutated
T5815 227-231 RB denotes next
T5816 240-243 DT denotes the
T5817 255-259 NN denotes part
T5818 244-245 NN denotes C
T5819 246-254 JJ denotes terminal
T5820 245-246 HYPH denotes -
T5821 260-262 IN denotes of
T5822 263-266 DT denotes the
T5823 271-275 NN denotes gene
T5824 267-270 NN denotes p53
T5825 276-278 IN denotes in
T5826 279-282 CD denotes two
T5827 283-289 NNS denotes rounds
T5828 290-292 IN denotes of
T5829 293-296 NN denotes PCR
T5830 297-308 NN denotes mutagenesis
T5831 308-310 , denotes ,
T5832 310-315 RB denotes first
T5833 316-320 IN denotes with
T5834 321-328 NNS denotes primers
T5835 332-368 NN denotes GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
T5836 329-330 CD denotes 5
T5837 330-331 SYM denotes ′
T5838 331-332 HYPH denotes -
T5839 368-369 HYPH denotes -
T5840 369-370 CD denotes 3
T5841 370-371 SYM denotes ′
T5842 372-375 CC denotes and
T5843 376-377 CD denotes 5
T5844 379-415 NN denotes GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
T5845 377-378 SYM denotes ′
T5846 378-379 HYPH denotes -
T5847 415-416 HYPH denotes -
T5848 416-417 CD denotes 3
T5849 417-418 SYM denotes ′
T5850 418-420 , denotes ,
T5851 420-425 WDT denotes which
T5852 426-433 VBD denotes removed
T5853 434-437 DT denotes the
T5854 443-448 NN denotes codon
T5855 438-442 NN denotes stop
T5856 449-452 CC denotes and
T5857 453-463 VBD denotes introduced
T5858 464-465 DT denotes a
T5859 472-476 NN denotes site
T5860 466-471 NN denotes BamHI
T5861 476-478 , denotes ,
T5862 478-482 RB denotes then
T5863 483-487 IN denotes with
T5864 488-495 NNS denotes primers
T5865 499-533 NN denotes GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
T5866 496-497 CD denotes 5
T5867 497-498 SYM denotes ′
T5868 498-499 HYPH denotes -
T5869 533-534 HYPH denotes -
T5870 534-535 CD denotes 3
T5871 535-536 SYM denotes ′
T5872 537-540 CC denotes and
T5873 541-542 CD denotes 5
T5874 544-578 NN denotes TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
T5875 542-543 SYM denotes ′
T5876 543-544 HYPH denotes -
T5877 578-579 HYPH denotes -
T5878 579-580 CD denotes 3
T5879 580-581 SYM denotes ′
T5880 581-583 , denotes ,
T5881 583-588 WDT denotes which
T5882 589-599 VBD denotes introduced
T5883 600-602 DT denotes an
T5884 609-613 NN denotes site
T5885 603-608 NN denotes EcoRI
T5886 613-614 . denotes .
T5887 614-801 sentence denotes We verified the sequence from the mutated plasmid, then digested it with BamHI and EcoRI, to insert in frame GFP sequences from a Bam HI-EcoRI fragment of plasmid phr-GFP-1 (Stratagene).
T5888 615-617 PRP denotes We
T5889 618-626 VBD denotes verified
T5890 627-630 DT denotes the
T5891 631-639 NN denotes sequence
T5892 640-644 IN denotes from
T5893 645-648 DT denotes the
T5894 657-664 NN denotes plasmid
T5895 649-656 VBN denotes mutated
T5896 664-666 , denotes ,
T5897 666-670 RB denotes then
T5898 671-679 VBD denotes digested
T5899 680-682 PRP denotes it
T5900 683-687 IN denotes with
T5901 688-693 NN denotes BamHI
T5902 694-697 CC denotes and
T5903 698-703 NN denotes EcoRI
T5904 703-705 , denotes ,
T5905 705-707 TO denotes to
T5906 708-714 VB denotes insert
T5907 715-717 IN denotes in
T5908 718-723 NN denotes frame
T5909 724-727 NN denotes GFP
T5910 728-737 NNS denotes sequences
T5911 738-742 IN denotes from
T5912 743-744 DT denotes a
T5913 758-766 NN denotes fragment
T5914 745-748 NN denotes Bam
T5915 752-757 NN denotes EcoRI
T5916 749-751 NN denotes HI
T5917 751-752 HYPH denotes -
T5918 767-769 IN denotes of
T5919 770-777 NN denotes plasmid
T5920 782-785 NN denotes GFP
T5921 778-781 NN denotes phr
T5922 781-782 HYPH denotes -
T5923 785-786 HYPH denotes -
T5924 786-787 CD denotes 1
T5925 788-789 -LRB- denotes (
T5926 789-799 NNP denotes Stratagene
T5927 799-800 -RRB- denotes )
T5928 800-801 . denotes .
T5929 801-1044 sentence denotes We verified the sequence of this p53-GFP fusion fragment, then swapped it in the L3-p53PmlEagPuroΔTK-1L plasmid (see above) by HindIII and FseI digestion, resulting in the p53GFP exchange construct, the sequence which was verified before use.
T5930 802-804 PRP denotes We
T5931 805-813 VBD denotes verified
T5932 814-817 DT denotes the
T5933 818-826 NN denotes sequence
T5934 827-829 IN denotes of
T5935 830-834 DT denotes this
T5936 850-858 NN denotes fragment
T5937 835-838 NN denotes p53
T5938 839-842 NN denotes GFP
T5939 838-839 HYPH denotes -
T5940 843-849 NN denotes fusion
T5941 858-860 , denotes ,
T5942 860-864 RB denotes then
T5943 865-872 VBD denotes swapped
T5944 873-875 PRP denotes it
T5945 876-878 IN denotes in
T5946 879-882 DT denotes the
T5947 906-913 NN denotes plasmid
T5948 883-885 NN denotes L3
T5949 903-905 NN denotes 1L
T5950 885-886 HYPH denotes -
T5951 886-902 NN denotes p53PmlEagPuroΔTK
T5952 902-903 HYPH denotes -
T5953 914-915 -LRB- denotes (
T5954 915-918 VB denotes see
T5955 919-924 RB denotes above
T5956 924-925 -RRB- denotes )
T5957 926-928 IN denotes by
T5958 929-936 NN denotes HindIII
T5959 946-955 NN denotes digestion
T5960 937-940 CC denotes and
T5961 941-945 NN denotes FseI
T5962 955-957 , denotes ,
T5963 957-966 VBG denotes resulting
T5964 967-969 IN denotes in
T5965 970-973 DT denotes the
T5966 990-999 NN denotes construct
T5967 974-980 NN denotes p53GFP
T5968 981-989 NN denotes exchange
T5969 999-1001 , denotes ,
T5970 1001-1004 DT denotes the
T5971 1005-1013 NN denotes sequence
T5972 1014-1019 WDT denotes which
T5973 1024-1032 VBN denotes verified
T5974 1020-1023 VBD denotes was
T5975 1033-1039 IN denotes before
T5976 1040-1043 NN denotes use
T5977 1043-1044 . denotes .
T5978 1044-1220 sentence denotes The p53ΔPGFP exchange construct was engineered by combining sequences from the p53GFP exchange plasmid and sequences from the p53ΔP targeting construct described recently (5).
T5979 1045-1048 DT denotes The
T5980 1067-1076 NN denotes construct
T5981 1049-1057 NN denotes p53ΔPGFP
T5982 1058-1066 NN denotes exchange
T5983 1081-1091 VBN denotes engineered
T5984 1077-1080 VBD denotes was
T5985 1092-1094 IN denotes by
T5986 1095-1104 VBG denotes combining
T5987 1105-1114 NNS denotes sequences
T5988 1115-1119 IN denotes from
T5989 1120-1123 DT denotes the
T5990 1140-1147 NN denotes plasmid
T5991 1124-1130 NN denotes p53GFP
T5992 1131-1139 NN denotes exchange
T5993 1148-1151 CC denotes and
T5994 1152-1161 NNS denotes sequences
T5995 1162-1166 IN denotes from
T5996 1167-1170 DT denotes the
T5997 1187-1196 NN denotes construct
T5998 1171-1176 NN denotes p53ΔP
T5999 1177-1186 NN denotes targeting
T6000 1197-1206 VBN denotes described
T6001 1207-1215 RB denotes recently
T6002 1216-1217 -LRB- denotes (
T6003 1217-1218 CD denotes 5
T6004 1218-1219 -RRB- denotes )
T6005 1219-1220 . denotes .
T6006 1220-1263 sentence denotes Its sequence was also verified before use.
T6007 1221-1224 PRP$ denotes Its
T6008 1225-1233 NN denotes sequence
T6009 1243-1251 VBN denotes verified
T6010 1234-1237 VBD denotes was
T6011 1238-1242 RB denotes also
T6012 1252-1258 IN denotes before
T6013 1259-1262 NN denotes use
T6014 1262-1263 . denotes .
R1628 T5764 T5765 aux To,make
R1629 T5765 T5766 advcl make,subcloned
R1630 T5767 T5768 det a,protein
R1631 T5768 T5765 dobj protein,make
R1632 T5769 T5770 compound p53,GFP
R1633 T5770 T5768 compound GFP,protein
R1634 T5771 T5770 punct -,GFP
R1635 T5772 T5768 compound fusion,protein
R1636 T5773 T5766 punct ", ",subcloned
R1637 T5774 T5766 nsubj we,subcloned
R1638 T5775 T5766 advmod first,subcloned
R1639 T5776 T5777 det a,fragment
R1640 T5777 T5766 dobj fragment,subcloned
R1641 T5778 T5779 compound SacII,HindIII
R1642 T5779 T5777 compound HindIII,fragment
R1643 T5780 T5779 punct -,HindIII
R1644 T5781 T5777 prep of,fragment
R1645 T5782 T5783 det the,locus
R1646 T5783 T5781 pobj locus,of
R1647 T5784 T5783 compound p53,locus
R1648 T5785 T5777 punct (,fragment
R1649 T5786 T5777 acl corresponding,fragment
R1650 T5787 T5786 prep to,corresponding
R1651 T5788 T5787 pobj part,to
R1652 T5789 T5788 prep of,part
R1653 T5790 T5789 pobj exon,of
R1654 T5791 T5790 nummod 10,exon
R1655 T5792 T5786 prep to,corresponding
R1656 T5793 T5792 pobj sequences,to
R1657 T5794 T5793 advmod downstream,sequences
R1658 T5795 T5794 prep of,downstream
R1659 T5796 T5797 det the,gene
R1660 T5797 T5795 pobj gene,of
R1661 T5798 T5766 punct ),subcloned
R1662 T5799 T5766 prep into,subcloned
R1663 T5800 T5799 pobj pBS,into
R1664 T5801 T5766 punct ", ",subcloned
R1665 T5802 T5803 advmod then,mutated
R1666 T5803 T5766 dep mutated,subcloned
R1667 T5804 T5805 det the,site
R1668 T5805 T5803 dobj site,mutated
R1669 T5806 T5805 compound HindIII,site
R1670 T5807 T5803 prep into,mutated
R1671 T5808 T5809 det a,site
R1672 T5809 T5807 pobj site,into
R1673 T5810 T5809 compound FseI,site
R1674 T5811 T5766 punct .,subcloned
R1675 T5813 T5814 nsubj We,mutated
R1676 T5815 T5814 advmod next,mutated
R1677 T5816 T5817 det the,part
R1678 T5817 T5814 dobj part,mutated
R1679 T5818 T5819 npadvmod C,terminal
R1680 T5819 T5817 amod terminal,part
R1681 T5820 T5819 punct -,terminal
R1682 T5821 T5817 prep of,part
R1683 T5822 T5823 det the,gene
R1684 T5823 T5821 pobj gene,of
R1685 T5824 T5823 compound p53,gene
R1686 T5825 T5814 prep in,mutated
R1687 T5826 T5827 nummod two,rounds
R1688 T5827 T5825 pobj rounds,in
R1689 T5828 T5827 prep of,rounds
R1690 T5829 T5830 compound PCR,mutagenesis
R1691 T5830 T5828 pobj mutagenesis,of
R1692 T5831 T5814 punct ", ",mutated
R1693 T5832 T5833 advmod first,with
R1694 T5833 T5814 prep with,mutated
R1695 T5834 T5835 nmod primers,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1696 T5835 T5833 pobj GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC,with
R1697 T5836 T5835 nummod 5,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1698 T5837 T5836 punct ′,5
R1699 T5838 T5835 punct -,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1700 T5839 T5835 punct -,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1701 T5840 T5835 nummod 3,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1702 T5841 T5835 punct ′,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1703 T5842 T5835 cc and,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1704 T5843 T5844 nummod 5,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1705 T5844 T5835 conj GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1706 T5845 T5843 punct ′,5
R1707 T5846 T5844 punct -,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1708 T5847 T5844 punct -,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1709 T5848 T5844 nummod 3,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1710 T5849 T5844 punct ′,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1711 T5850 T5835 punct ", ",GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1712 T5851 T5852 dep which,removed
R1713 T5852 T5835 relcl removed,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1714 T5853 T5854 det the,codon
R1715 T5854 T5852 dobj codon,removed
R1716 T5855 T5854 compound stop,codon
R1717 T5856 T5852 cc and,removed
R1718 T5857 T5852 conj introduced,removed
R1719 T5858 T5859 det a,site
R1720 T5859 T5857 dobj site,introduced
R1721 T5860 T5859 compound BamHI,site
R1722 T5861 T5833 punct ", ",with
R1723 T5862 T5863 advmod then,with
R1724 T5863 T5833 prep with,with
R1725 T5864 T5865 nmod primers,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1726 T5865 T5863 pobj GACGGATCCCCTCTGAATTCCGTCCCCATCACCA,with
R1727 T5866 T5865 nummod 5,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1728 T5867 T5866 punct ′,5
R1729 T5868 T5865 punct -,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1730 T5869 T5865 punct -,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1731 T5870 T5865 nummod 3,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1732 T5871 T5865 punct ′,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1733 T5872 T5865 cc and,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1734 T5873 T5874 nummod 5,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1735 T5874 T5865 conj TGGTGATGGGGACGGAATACAGAGGGGATCCGTC,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1736 T5875 T5873 punct ′,5
R1737 T5876 T5874 punct -,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1738 T5877 T5874 punct -,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1739 T5878 T5874 nummod 3,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1740 T5879 T5874 punct ′,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1741 T5880 T5865 punct ", ",GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1742 T5881 T5882 dep which,introduced
R1743 T5882 T5865 relcl introduced,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1744 T5883 T5884 det an,site
R1745 T5884 T5882 dobj site,introduced
R1746 T5885 T5884 compound EcoRI,site
R1747 T5886 T5814 punct .,mutated
R1748 T5888 T5889 nsubj We,verified
R1749 T5890 T5891 det the,sequence
R1750 T5891 T5889 dobj sequence,verified
R1751 T5892 T5891 prep from,sequence
R1752 T5893 T5894 det the,plasmid
R1753 T5894 T5892 pobj plasmid,from
R1754 T5895 T5894 amod mutated,plasmid
R1755 T5896 T5889 punct ", ",verified
R1756 T5897 T5898 advmod then,digested
R1757 T5898 T5889 dep digested,verified
R1758 T5899 T5898 dobj it,digested
R1759 T5900 T5898 prep with,digested
R1760 T5901 T5900 pobj BamHI,with
R1761 T5902 T5901 cc and,BamHI
R1762 T5903 T5901 conj EcoRI,BamHI
R1763 T5904 T5889 punct ", ",verified
R1764 T5905 T5906 aux to,insert
R1765 T5906 T5889 advcl insert,verified
R1766 T5907 T5906 prep in,insert
R1767 T5908 T5909 compound frame,GFP
R1768 T5909 T5910 compound GFP,sequences
R1769 T5910 T5907 pobj sequences,in
R1770 T5911 T5910 prep from,sequences
R1771 T5912 T5913 det a,fragment
R1772 T5913 T5911 pobj fragment,from
R1773 T5914 T5915 compound Bam,EcoRI
R1774 T5915 T5913 compound EcoRI,fragment
R1775 T5916 T5915 compound HI,EcoRI
R1776 T5917 T5915 punct -,EcoRI
R1777 T5918 T5913 prep of,fragment
R1778 T5919 T5920 compound plasmid,GFP
R1779 T5920 T5918 pobj GFP,of
R1780 T5921 T5920 compound phr,GFP
R1781 T5922 T5920 punct -,GFP
R1782 T5923 T5920 punct -,GFP
R1783 T5924 T5920 nummod 1,GFP
R1784 T5925 T5926 punct (,Stratagene
R1785 T5926 T5913 parataxis Stratagene,fragment
R1786 T5927 T5926 punct ),Stratagene
R1787 T5928 T5889 punct .,verified
R1788 T5930 T5931 nsubj We,verified
R1789 T5932 T5933 det the,sequence
R1790 T5933 T5931 dobj sequence,verified
R1791 T5934 T5933 prep of,sequence
R1792 T5935 T5936 det this,fragment
R1793 T5936 T5934 pobj fragment,of
R1794 T5937 T5938 compound p53,GFP
R1795 T5938 T5940 compound GFP,fusion
R1796 T5939 T5938 punct -,GFP
R1797 T5940 T5936 compound fusion,fragment
R1798 T5941 T5931 punct ", ",verified
R1799 T5942 T5943 advmod then,swapped
R1800 T5943 T5931 dep swapped,verified
R1801 T5944 T5943 dobj it,swapped
R1802 T5945 T5943 prep in,swapped
R1803 T5946 T5947 det the,plasmid
R1804 T5947 T5945 pobj plasmid,in
R1805 T5948 T5949 compound L3,1L
R1806 T5949 T5947 compound 1L,plasmid
R1807 T5950 T5949 punct -,1L
R1808 T5951 T5949 compound p53PmlEagPuroΔTK,1L
R1809 T5952 T5949 punct -,1L
R1810 T5953 T5954 punct (,see
R1811 T5954 T5943 parataxis see,swapped
R1812 T5955 T5954 advmod above,see
R1813 T5956 T5954 punct ),see
R1814 T5957 T5943 prep by,swapped
R1815 T5958 T5959 nmod HindIII,digestion
R1816 T5959 T5957 pobj digestion,by
R1817 T5960 T5958 cc and,HindIII
R1818 T5961 T5958 conj FseI,HindIII
R1819 T5962 T5943 punct ", ",swapped
R1820 T5963 T5943 advcl resulting,swapped
R1821 T5964 T5963 prep in,resulting
R1822 T5965 T5966 det the,construct
R1823 T5966 T5964 pobj construct,in
R1824 T5967 T5968 compound p53GFP,exchange
R1825 T5968 T5966 compound exchange,construct
R1826 T5969 T5966 punct ", ",construct
R1827 T5970 T5971 det the,sequence
R1828 T5971 T5966 appos sequence,construct
R1829 T5972 T5973 dep which,verified
R1830 T5973 T5971 relcl verified,sequence
R1831 T5974 T5973 auxpass was,verified
R1832 T5975 T5973 prep before,verified
R1833 T5976 T5975 pobj use,before
R1834 T5977 T5931 punct .,verified
R1835 T5979 T5980 det The,construct
R1836 T5980 T5983 nsubjpass construct,engineered
R1837 T5981 T5982 compound p53ΔPGFP,exchange
R1838 T5982 T5980 compound exchange,construct
R1839 T5984 T5983 auxpass was,engineered
R1840 T5985 T5983 prep by,engineered
R1841 T5986 T5985 pcomp combining,by
R1842 T5987 T5986 dobj sequences,combining
R1843 T5988 T5987 prep from,sequences
R1844 T5989 T5990 det the,plasmid
R1845 T5990 T5988 pobj plasmid,from
R1846 T5991 T5990 compound p53GFP,plasmid
R1847 T5992 T5990 compound exchange,plasmid
R1848 T5993 T5987 cc and,sequences
R1849 T5994 T5987 conj sequences,sequences
R1850 T5995 T5994 prep from,sequences
R1851 T5996 T5997 det the,construct
R1852 T5997 T5995 pobj construct,from
R1853 T5998 T5997 compound p53ΔP,construct
R1854 T5999 T5997 compound targeting,construct
R1855 T6000 T5987 acl described,sequences
R1856 T6001 T6000 advmod recently,described
R1857 T6002 T6003 punct (,5
R1858 T6003 T5986 parataxis 5,combining
R1859 T6004 T6003 punct ),5
R1860 T6005 T5983 punct .,engineered
R1861 T6007 T6008 poss Its,sequence
R1862 T6008 T6009 nsubjpass sequence,verified
R1863 T6010 T6009 auxpass was,verified
R1864 T6011 T6009 advmod also,verified
R1865 T6012 T6009 prep before,verified
R1866 T6013 T6012 pobj use,before
R1867 T6014 T6009 punct .,verified

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T5702 10-13 PR_EXT:000003035 denotes p53
T5703 18-24 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T5704 25-32 CHEBI_PR_EXT:protein denotes protein
T5705 61-68 PR_EXT:P43870 denotes HindIII
T5706 85-88 PR_EXT:000003035 denotes p53
T5707 113-125 SO_EXT:0000852 denotes part of exon
T5708 132-141 SO_EXT:biological_sequence denotes sequences
T5709 142-152 SO_EXT:sequence_downstreamness denotes downstream
T5710 160-164 SO_EXT:0000704 denotes gene
T5711 181-188 SO_EXT:sequence_alteration_process denotes mutated
T5712 193-200 PR_EXT:P43870 denotes HindIII
T5713 232-239 SO_EXT:sequence_alteration_process denotes mutated
T5714 244-254 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T5716 255-262,271-275 SO_EXT:0000831 denotes part of gene
T5717 267-270 PR_EXT:000003035 denotes p53
T5718 297-308 SO_EXT:sequence_alteration_process denotes mutagenesis
T5719 321-328 SO_EXT:0000112 denotes primers
T5720 438-448 SO_EXT:0000319 denotes stop codon
T5721 466-471 PR_EXT:P23940 denotes BamHI
T5722 488-495 SO_EXT:0000112 denotes primers
T5723 631-639 SO_EXT:biological_sequence denotes sequence
T5724 649-656 SO_EXT:sequence_alteration_process denotes mutated
T5725 657-664 SO_EXT:0000155 denotes plasmid
T5726 688-693 PR_EXT:P23940 denotes BamHI
T5727 708-714 SO_EXT:sequence_insertion_process denotes insert
T5728 715-723 SO:0001817 denotes in frame
T5729 728-737 SO_EXT:biological_sequence denotes sequences
T5730 745-751 PR_EXT:P23940 denotes Bam HI
T5731 770-777 SO_EXT:0000155 denotes plasmid
T5732 818-826 SO_EXT:biological_sequence denotes sequence
T5733 835-838 PR_EXT:000003035 denotes p53
T5734 843-849 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T5735 906-913 SO_EXT:0000155 denotes plasmid
T5736 929-936 PR_EXT:P43870 denotes HindIII
T5737 974-977 PR_EXT:000003035 denotes p53
T5738 981-989 SO_EXT:sequence_substitution_process denotes exchange
T5739 990-999 SO_EXT:engineered_biological_sequence denotes construct
T5740 1005-1013 SO_EXT:biological_sequence denotes sequence
T5741 1049-1052 PR_EXT:000003035 denotes p53
T5742 1058-1066 SO_EXT:sequence_substitution_process denotes exchange
T5743 1067-1076 SO_EXT:engineered_biological_sequence denotes construct
T5744 1105-1114 SO_EXT:biological_sequence denotes sequences
T5745 1124-1127 PR_EXT:000003035 denotes p53
T5746 1131-1139 SO_EXT:sequence_substitution_process denotes exchange
T5747 1140-1147 SO_EXT:0000155 denotes plasmid
T5748 1152-1161 SO_EXT:biological_sequence denotes sequences
T5749 1171-1174 PR_EXT:000003035 denotes p53
T5750 1187-1196 SO_EXT:engineered_biological_sequence denotes construct
T5751 1225-1233 SO_EXT:biological_sequence denotes sequence

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T5671 10-13 PR:000003035 denotes p53
T5672 61-68 PR:P43870 denotes HindIII
T5673 85-88 PR:000003035 denotes p53
T5674 113-125 SO:0000852 denotes part of exon
T5675 160-164 SO:0000704 denotes gene
T5676 193-200 PR:P43870 denotes HindIII
T5678 255-262,271-275 SO:0000831 denotes part of gene
T5679 267-270 PR:000003035 denotes p53
T5680 321-328 SO:0000112 denotes primers
T5681 438-448 SO:0000319 denotes stop codon
T5682 466-471 PR:P23940 denotes BamHI
T5683 488-495 SO:0000112 denotes primers
T5684 657-664 SO:0000155 denotes plasmid
T5685 688-693 PR:P23940 denotes BamHI
T5686 715-723 SO:0001817 denotes in frame
T5687 745-751 PR:P23940 denotes Bam HI
T5688 770-777 SO:0000155 denotes plasmid
T5689 835-838 PR:000003035 denotes p53
T5690 906-913 SO:0000155 denotes plasmid
T5691 929-936 PR:P43870 denotes HindIII
T5692 974-977 PR:000003035 denotes p53
T5693 1049-1052 PR:000003035 denotes p53
T5694 1124-1127 PR:000003035 denotes p53
T5695 1140-1147 SO:0000155 denotes plasmid
T5696 1171-1174 PR:000003035 denotes p53

2_test

Id Subject Object Predicate Lexical cue
16870721-16616333-76660903 1217-1218 16616333 denotes 5