PMC:1540739 / 6650-7240 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T4718","span":{"begin":120,"end":128},"obj":"VBD"},{"id":"T4719","span":{"begin":7,"end":9},"obj":"PRP"},{"id":"T4720","span":{"begin":13,"end":22},"obj":"VB"},{"id":"T4721","span":{"begin":10,"end":12},"obj":"TO"},{"id":"T4722","span":{"begin":23,"end":27},"obj":"IN"},{"id":"T4723","span":{"begin":28,"end":30},"obj":"NN"},{"id":"T4724","span":{"begin":31,"end":35},"obj":"NN"},{"id":"T4725","span":{"begin":35,"end":37},"obj":","},{"id":"T4726","span":{"begin":37,"end":45},"obj":"VBG"},{"id":"T4727","span":{"begin":46,"end":55},"obj":"JJ"},{"id":"T4728","span":{"begin":56,"end":60},"obj":"NN"},{"id":"T4729","span":{"begin":61,"end":63},"obj":"IN"},{"id":"T4730","span":{"begin":64,"end":71},"obj":"JJ"},{"id":"T4731","span":{"begin":77,"end":82},"obj":"NNS"},{"id":"T4732","span":{"begin":72,"end":76},"obj":"NN"},{"id":"T4733","span":{"begin":82,"end":84},"obj":":"},{"id":"T4734","span":{"begin":84,"end":92},"obj":"JJ"},{"id":"T4735","span":{"begin":93,"end":97},"obj":"NN"},{"id":"T4736","span":{"begin":98,"end":102},"obj":"IN"},{"id":"T4737","span":{"begin":103,"end":108},"obj":"DT"},{"id":"T4738","span":{"begin":114,"end":119},"obj":"NNS"},{"id":"T4739","span":{"begin":109,"end":113},"obj":"NN"},{"id":"T4740","span":{"begin":129,"end":133},"obj":"IN"},{"id":"T4741","span":{"begin":134,"end":136},"obj":"DT"},{"id":"T4742","span":{"begin":145,"end":154},"obj":"NN"},{"id":"T4743","span":{"begin":137,"end":144},"obj":"JJ"},{"id":"T4744","span":{"begin":155,"end":157},"obj":"IN"},{"id":"T4745","span":{"begin":158,"end":160},"obj":"CD"},{"id":"T4746","span":{"begin":160,"end":161},"obj":"NN"},{"id":"T4747","span":{"begin":162,"end":164},"obj":"IN"},{"id":"T4748","span":{"begin":165,"end":168},"obj":"CD"},{"id":"T4749","span":{"begin":169,"end":173},"obj":"NNS"},{"id":"T4750","span":{"begin":174,"end":176},"obj":"IN"},{"id":"T4751","span":{"begin":177,"end":180},"obj":"NN"},{"id":"T4752","span":{"begin":181,"end":186},"obj":"NNS"},{"id":"T4753","span":{"begin":187,"end":190},"obj":"CC"},{"id":"T4754","span":{"begin":191,"end":193},"obj":"DT"},{"id":"T4755","span":{"begin":202,"end":211},"obj":"NN"},{"id":"T4756","span":{"begin":194,"end":201},"obj":"JJ"},{"id":"T4757","span":{"begin":212,"end":214},"obj":"IN"},{"id":"T4758","span":{"begin":215,"end":217},"obj":"CD"},{"id":"T4759","span":{"begin":217,"end":218},"obj":"NN"},{"id":"T4760","span":{"begin":219,"end":221},"obj":"IN"},{"id":"T4761","span":{"begin":222,"end":226},"obj":"CD"},{"id":"T4762","span":{"begin":227,"end":231},"obj":"NNS"},{"id":"T4763","span":{"begin":232,"end":234},"obj":"IN"},{"id":"T4764","span":{"begin":235,"end":239},"obj":"NN"},{"id":"T4765","span":{"begin":240,"end":245},"obj":"NNS"},{"id":"T4766","span":{"begin":246,"end":247},"obj":"-LRB-"},{"id":"T4767","span":{"begin":247,"end":249},"obj":"CD"},{"id":"T4768","span":{"begin":249,"end":250},"obj":"-RRB-"},{"id":"T4769","span":{"begin":250,"end":251},"obj":"."},{"id":"T4770","span":{"begin":251,"end":375},"obj":"sentence"},{"id":"T4771","span":{"begin":252,"end":255},"obj":"DT"},{"id":"T4772","span":{"begin":262,"end":269},"obj":"NN"},{"id":"T4773","span":{"begin":256,"end":258},"obj":"NN"},{"id":"T4774","span":{"begin":259,"end":261},"obj":"NN"},{"id":"T4775","span":{"begin":258,"end":259},"obj":"HYPH"},{"id":"T4776","span":{"begin":280,"end":288},"obj":"VBN"},{"id":"T4777","span":{"begin":270,"end":273},"obj":"VBD"},{"id":"T4778","span":{"begin":274,"end":279},"obj":"RB"},{"id":"T4779","span":{"begin":289,"end":291},"obj":"TO"},{"id":"T4780","span":{"begin":292,"end":299},"obj":"VB"},{"id":"T4781","span":{"begin":300,"end":301},"obj":"DT"},{"id":"T4782","span":{"begin":302,"end":306},"obj":"NN"},{"id":"T4783","span":{"begin":307,"end":310},"obj":"CC"},{"id":"T4784","span":{"begin":311,"end":312},"obj":"DT"},{"id":"T4785","span":{"begin":318,"end":322},"obj":"NN"},{"id":"T4786","span":{"begin":313,"end":317},"obj":"NN"},{"id":"T4787","span":{"begin":323,"end":330},"obj":"IN"},{"id":"T4788","span":{"begin":331,"end":334},"obj":"DT"},{"id":"T4789","span":{"begin":340,"end":345},"obj":"NNS"},{"id":"T4790","span":{"begin":335,"end":339},"obj":"NN"},{"id":"T4791","span":{"begin":345,"end":347},"obj":","},{"id":"T4792","span":{"begin":347,"end":354},"obj":"VBG"},{"id":"T4793","span":{"begin":355,"end":357},"obj":"IN"},{"id":"T4794","span":{"begin":358,"end":365},"obj":"NN"},{"id":"T4795","span":{"begin":372,"end":374},"obj":"NN"},{"id":"T4796","span":{"begin":366,"end":368},"obj":"NN"},{"id":"T4797","span":{"begin":368,"end":369},"obj":"HYPH"},{"id":"T4798","span":{"begin":369,"end":371},"obj":"NN"},{"id":"T4799","span":{"begin":371,"end":372},"obj":"HYPH"},{"id":"T4800","span":{"begin":374,"end":375},"obj":"."},{"id":"T4802","span":{"begin":376,"end":378},"obj":"PRP"},{"id":"T4803","span":{"begin":384,"end":392},"obj":"VBD"},{"id":"T4804","span":{"begin":379,"end":383},"obj":"RB"},{"id":"T4805","span":{"begin":393,"end":394},"obj":"DT"},{"id":"T4806","span":{"begin":403,"end":410},"obj":"NN"},{"id":"T4807","span":{"begin":395,"end":402},"obj":"NN"},{"id":"T4808","span":{"begin":411,"end":412},"obj":"-LRB-"},{"id":"T4809","span":{"begin":412,"end":417},"obj":"NN"},{"id":"T4810","span":{"begin":417,"end":419},"obj":","},{"id":"T4811","span":{"begin":419,"end":420},"obj":"DT"},{"id":"T4812","span":{"begin":426,"end":430},"obj":"NN"},{"id":"T4813","span":{"begin":421,"end":425},"obj":"NN"},{"id":"T4814","span":{"begin":431,"end":435},"obj":"IN"},{"id":"T4815","span":{"begin":436,"end":438},"obj":"NNP"},{"id":"T4816","span":{"begin":439,"end":446},"obj":"NNP"},{"id":"T4817","span":{"begin":446,"end":447},"obj":"-RRB-"},{"id":"T4818","span":{"begin":448,"end":450},"obj":"IN"},{"id":"T4819","span":{"begin":451,"end":456},"obj":"VBG"},{"id":"T4820","span":{"begin":457,"end":473},"obj":"NNS"},{"id":"T4821","span":{"begin":474,"end":476},"obj":"TO"},{"id":"T4822","span":{"begin":477,"end":484},"obj":"VB"},{"id":"T4823","span":{"begin":485,"end":486},"obj":"DT"},{"id":"T4824","span":{"begin":492,"end":496},"obj":"NN"},{"id":"T4825","span":{"begin":487,"end":491},"obj":"NN"},{"id":"T4826","span":{"begin":497,"end":507},"obj":"RB"},{"id":"T4827","span":{"begin":508,"end":510},"obj":"IN"},{"id":"T4828","span":{"begin":511,"end":514},"obj":"DT"},{"id":"T4829","span":{"begin":523,"end":527},"obj":"NN"},{"id":"T4830","span":{"begin":515,"end":522},"obj":"NN"},{"id":"T4831","span":{"begin":528,"end":531},"obj":"CC"},{"id":"T4832","span":{"begin":532,"end":541},"obj":"VB"},{"id":"T4833","span":{"begin":542,"end":543},"obj":"DT"},{"id":"T4834","span":{"begin":549,"end":553},"obj":"NN"},{"id":"T4835","span":{"begin":544,"end":548},"obj":"NN"},{"id":"T4836","span":{"begin":554,"end":562},"obj":"RB"},{"id":"T4837","span":{"begin":562,"end":564},"obj":","},{"id":"T4838","span":{"begin":564,"end":567},"obj":"CC"},{"id":"T4839","span":{"begin":568,"end":569},"obj":"DT"},{"id":"T4840","span":{"begin":575,"end":579},"obj":"NN"},{"id":"T4841","span":{"begin":570,"end":574},"obj":"NN"},{"id":"T4842","span":{"begin":580,"end":590},"obj":"RB"}],"relations":[{"id":"R1149","pred":"nsubj","subj":"T4719","obj":"T4720"},{"id":"R1151","pred":"aux","subj":"T4721","obj":"T4720"},{"id":"R1152","pred":"prep","subj":"T4722","obj":"T4720"},{"id":"R1153","pred":"compound","subj":"T4723","obj":"T4724"},{"id":"R1154","pred":"pobj","subj":"T4724","obj":"T4722"},{"id":"R1157","pred":"amod","subj":"T4727","obj":"T4728"},{"id":"R1158","pred":"dobj","subj":"T4728","obj":"T4726"},{"id":"R1159","pred":"prep","subj":"T4729","obj":"T4726"},{"id":"R1160","pred":"amod","subj":"T4730","obj":"T4731"},{"id":"R1161","pred":"pobj","subj":"T4731","obj":"T4729"},{"id":"R1162","pred":"compound","subj":"T4732","obj":"T4731"},{"id":"R1163","pred":"punct","subj":"T4733","obj":"T4718"},{"id":"R1164","pred":"amod","subj":"T4734","obj":"T4735"},{"id":"R1165","pred":"nsubj","subj":"T4735","obj":"T4718"},{"id":"R1166","pred":"prep","subj":"T4736","obj":"T4735"},{"id":"R1167","pred":"det","subj":"T4737","obj":"T4738"},{"id":"R1168","pred":"pobj","subj":"T4738","obj":"T4736"},{"id":"R1169","pred":"compound","subj":"T4739","obj":"T4738"},{"id":"R1170","pred":"prep","subj":"T4740","obj":"T4718"},{"id":"R1171","pred":"det","subj":"T4741","obj":"T4742"},{"id":"R1172","pred":"pobj","subj":"T4742","obj":"T4740"},{"id":"R1173","pred":"amod","subj":"T4743","obj":"T4742"},{"id":"R1174","pred":"prep","subj":"T4744","obj":"T4742"},{"id":"R1175","pred":"nummod","subj":"T4745","obj":"T4746"},{"id":"R1176","pred":"pobj","subj":"T4746","obj":"T4744"},{"id":"R1177","pred":"prep","subj":"T4747","obj":"T4742"},{"id":"R1178","pred":"nummod","subj":"T4748","obj":"T4749"},{"id":"R1179","pred":"pobj","subj":"T4749","obj":"T4747"},{"id":"R1180","pred":"prep","subj":"T4750","obj":"T4749"},{"id":"R1181","pred":"compound","subj":"T4751","obj":"T4752"},{"id":"R1182","pred":"pobj","subj":"T4752","obj":"T4750"},{"id":"R1183","pred":"cc","subj":"T4753","obj":"T4742"},{"id":"R1184","pred":"det","subj":"T4754","obj":"T4755"},{"id":"R1185","pred":"conj","subj":"T4755","obj":"T4742"},{"id":"R1186","pred":"amod","subj":"T4756","obj":"T4755"},{"id":"R1187","pred":"prep","subj":"T4757","obj":"T4755"},{"id":"R1188","pred":"nummod","subj":"T4758","obj":"T4759"},{"id":"R1189","pred":"pobj","subj":"T4759","obj":"T4757"},{"id":"R1190","pred":"prep","subj":"T4760","obj":"T4755"},{"id":"R1191","pred":"nummod","subj":"T4761","obj":"T4762"},{"id":"R1192","pred":"pobj","subj":"T4762","obj":"T4760"},{"id":"R1193","pred":"prep","subj":"T4763","obj":"T4762"},{"id":"R1194","pred":"compound","subj":"T4764","obj":"T4765"},{"id":"R1195","pred":"pobj","subj":"T4765","obj":"T4763"},{"id":"R1196","pred":"punct","subj":"T4766","obj":"T4767"},{"id":"R1197","pred":"parataxis","subj":"T4767","obj":"T4718"},{"id":"R1198","pred":"punct","subj":"T4768","obj":"T4767"},{"id":"R1199","pred":"punct","subj":"T4769","obj":"T4718"},{"id":"R1200","pred":"det","subj":"T4771","obj":"T4772"},{"id":"R1201","pred":"nsubjpass","subj":"T4772","obj":"T4776"},{"id":"R1202","pred":"compound","subj":"T4773","obj":"T4774"},{"id":"R1203","pred":"compound","subj":"T4774","obj":"T4772"},{"id":"R1204","pred":"punct","subj":"T4775","obj":"T4774"},{"id":"R1205","pred":"auxpass","subj":"T4777","obj":"T4776"},{"id":"R1206","pred":"advmod","subj":"T4778","obj":"T4776"},{"id":"R1207","pred":"aux","subj":"T4779","obj":"T4780"},{"id":"R1208","pred":"advcl","subj":"T4780","obj":"T4776"},{"id":"R1209","pred":"det","subj":"T4781","obj":"T4782"},{"id":"R1210","pred":"dobj","subj":"T4782","obj":"T4780"},{"id":"R1211","pred":"cc","subj":"T4783","obj":"T4782"},{"id":"R1212","pred":"det","subj":"T4784","obj":"T4785"},{"id":"R1213","pred":"conj","subj":"T4785","obj":"T4782"},{"id":"R1214","pred":"compound","subj":"T4786","obj":"T4785"},{"id":"R1215","pred":"prep","subj":"T4787","obj":"T4780"},{"id":"R1216","pred":"det","subj":"T4788","obj":"T4789"},{"id":"R1217","pred":"pobj","subj":"T4789","obj":"T4787"},{"id":"R1218","pred":"compound","subj":"T4790","obj":"T4789"},{"id":"R1219","pred":"punct","subj":"T4791","obj":"T4776"},{"id":"R1220","pred":"advcl","subj":"T4792","obj":"T4776"},{"id":"R1221","pred":"prep","subj":"T4793","obj":"T4792"},{"id":"R1222","pred":"compound","subj":"T4794","obj":"T4795"},{"id":"R1223","pred":"pobj","subj":"T4795","obj":"T4793"},{"id":"R1224","pred":"compound","subj":"T4796","obj":"T4795"},{"id":"R1225","pred":"punct","subj":"T4797","obj":"T4795"},{"id":"R1226","pred":"compound","subj":"T4798","obj":"T4795"},{"id":"R1227","pred":"punct","subj":"T4799","obj":"T4795"},{"id":"R1228","pred":"punct","subj":"T4800","obj":"T4776"},{"id":"R1229","pred":"nsubj","subj":"T4802","obj":"T4803"},{"id":"R1230","pred":"advmod","subj":"T4804","obj":"T4803"},{"id":"R1231","pred":"det","subj":"T4805","obj":"T4806"},{"id":"R1232","pred":"dobj","subj":"T4806","obj":"T4803"},{"id":"R1233","pred":"compound","subj":"T4807","obj":"T4806"},{"id":"R1234","pred":"punct","subj":"T4808","obj":"T4806"},{"id":"R1235","pred":"appos","subj":"T4809","obj":"T4806"},{"id":"R1236","pred":"punct","subj":"T4810","obj":"T4809"},{"id":"R1237","pred":"det","subj":"T4811","obj":"T4812"},{"id":"R1238","pred":"appos","subj":"T4812","obj":"T4809"},{"id":"R1239","pred":"compound","subj":"T4813","obj":"T4812"},{"id":"R1240","pred":"prep","subj":"T4814","obj":"T4812"},{"id":"R1241","pred":"compound","subj":"T4815","obj":"T4816"},{"id":"R1242","pred":"pobj","subj":"T4816","obj":"T4814"},{"id":"R1243","pred":"punct","subj":"T4817","obj":"T4803"},{"id":"R1244","pred":"prep","subj":"T4818","obj":"T4803"},{"id":"R1245","pred":"pcomp","subj":"T4819","obj":"T4818"},{"id":"R1246","pred":"dobj","subj":"T4820","obj":"T4819"},{"id":"R1247","pred":"aux","subj":"T4821","obj":"T4822"},{"id":"R1248","pred":"advcl","subj":"T4822","obj":"T4819"},{"id":"R1249","pred":"det","subj":"T4823","obj":"T4824"},{"id":"R1250","pred":"dobj","subj":"T4824","obj":"T4822"},{"id":"R1251","pred":"compound","subj":"T4825","obj":"T4824"},{"id":"R1252","pred":"advmod","subj":"T4826","obj":"T4824"},{"id":"R1253","pred":"prep","subj":"T4827","obj":"T4826"},{"id":"R1254","pred":"det","subj":"T4828","obj":"T4829"},{"id":"R1255","pred":"pobj","subj":"T4829","obj":"T4827"},{"id":"R1256","pred":"compound","subj":"T4830","obj":"T4829"},{"id":"R1257","pred":"cc","subj":"T4831","obj":"T4822"},{"id":"R1258","pred":"conj","subj":"T4832","obj":"T4822"},{"id":"R1259","pred":"det","subj":"T4833","obj":"T4834"},{"id":"R1260","pred":"dobj","subj":"T4834","obj":"T4832"},{"id":"R1261","pred":"compound","subj":"T4835","obj":"T4834"},{"id":"R1262","pred":"advmod","subj":"T4836","obj":"T4832"},{"id":"R1263","pred":"punct","subj":"T4837","obj":"T4832"},{"id":"R1264","pred":"cc","subj":"T4838","obj":"T4832"},{"id":"R1265","pred":"det","subj":"T4839","obj":"T4840"},{"id":"R1266","pred":"conj","subj":"T4840","obj":"T4832"},{"id":"R1267","pred":"compound","subj":"T4841","obj":"T4840"},{"id":"R1268","pred":"advmod","subj":"T4842","obj":"T4840"}],"text":"revent it to recombine with WT loxP, ensuring efficient RMCE in several cell lines: accurate RMCE with these loxP sites occurred with an average frequency of 81% at two loci in CHO cells and an average frequency of 69% at four loci in Hela cells (14). The L3-1L plasmid was first modified to include a ClaI and a FseI site between the LoxP sites, leading to plasmid L3-CF-1L. We next modified a puroΔTK plasmid (YTC37, a kind gift from A. Bradley) by using oligonucleotides to destroy a NotI site downstream of the puroΔTK gene and introduce a NotI site upstream, and a FseI site downstream"}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T4542","span":{"begin":13,"end":22},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T4543","span":{"begin":28,"end":30},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T4544","span":{"begin":31,"end":35},"obj":"SO_EXT:0000346"},{"id":"T4545","span":{"begin":72,"end":76},"obj":"CL_GO_EXT:cell"},{"id":"T4546","span":{"begin":109,"end":119},"obj":"SO_EXT:0000346"},{"id":"T4547","span":{"begin":181,"end":186},"obj":"CL_GO_EXT:cell"},{"id":"T4548","span":{"begin":240,"end":245},"obj":"CL_GO_EXT:cell"},{"id":"T4549","span":{"begin":262,"end":269},"obj":"SO_EXT:0000155"},{"id":"T4550","span":{"begin":280,"end":288},"obj":"SO_EXT:sequence_alteration_process"},{"id":"T4551","span":{"begin":335,"end":345},"obj":"SO_EXT:0000346"},{"id":"T4552","span":{"begin":358,"end":365},"obj":"SO_EXT:0000155"},{"id":"T4553","span":{"begin":384,"end":392},"obj":"SO_EXT:sequence_alteration_process"},{"id":"T4554","span":{"begin":403,"end":410},"obj":"SO_EXT:0000155"},{"id":"T4555","span":{"begin":457,"end":473},"obj":"CHEBI_SO_EXT:oligonucleotide"},{"id":"T4556","span":{"begin":497,"end":507},"obj":"SO_EXT:sequence_downstreamness"},{"id":"T4557","span":{"begin":523,"end":527},"obj":"SO_EXT:0000704"},{"id":"T4558","span":{"begin":554,"end":562},"obj":"SO_EXT:sequence_upstreamness"},{"id":"T4559","span":{"begin":580,"end":590},"obj":"SO_EXT:sequence_downstreamness"}],"text":"revent it to recombine with WT loxP, ensuring efficient RMCE in several cell lines: accurate RMCE with these loxP sites occurred with an average frequency of 81% at two loci in CHO cells and an average frequency of 69% at four loci in Hela cells (14). The L3-1L plasmid was first modified to include a ClaI and a FseI site between the LoxP sites, leading to plasmid L3-CF-1L. We next modified a puroΔTK plasmid (YTC37, a kind gift from A. Bradley) by using oligonucleotides to destroy a NotI site downstream of the puroΔTK gene and introduce a NotI site upstream, and a FseI site downstream"}

{"project":"craft-ca-core-dev","denotations":[{"id":"T4485","span":{"begin":31,"end":35},"obj":"SO:0000346"},{"id":"T4486","span":{"begin":109,"end":119},"obj":"SO:0000346"},{"id":"T4487","span":{"begin":262,"end":269},"obj":"SO:0000155"},{"id":"T4488","span":{"begin":335,"end":345},"obj":"SO:0000346"},{"id":"T4489","span":{"begin":358,"end":365},"obj":"SO:0000155"},{"id":"T4490","span":{"begin":403,"end":410},"obj":"SO:0000155"},{"id":"T4491","span":{"begin":523,"end":527},"obj":"SO:0000704"}],"text":"revent it to recombine with WT loxP, ensuring efficient RMCE in several cell lines: accurate RMCE with these loxP sites occurred with an average frequency of 81% at two loci in CHO cells and an average frequency of 69% at four loci in Hela cells (14). The L3-1L plasmid was first modified to include a ClaI and a FseI site between the LoxP sites, leading to plasmid L3-CF-1L. We next modified a puroΔTK plasmid (YTC37, a kind gift from A. Bradley) by using oligonucleotides to destroy a NotI site downstream of the puroΔTK gene and introduce a NotI site upstream, and a FseI site downstream"}

{"project":"2_test","denotations":[{"id":"16870721-16204450-76660902","span":{"begin":247,"end":249},"obj":"16204450"}],"text":"revent it to recombine with WT loxP, ensuring efficient RMCE in several cell lines: accurate RMCE with these loxP sites occurred with an average frequency of 81% at two loci in CHO cells and an average frequency of 69% at four loci in Hela cells (14). The L3-1L plasmid was first modified to include a ClaI and a FseI site between the LoxP sites, leading to plasmid L3-CF-1L. We next modified a puroΔTK plasmid (YTC37, a kind gift from A. Bradley) by using oligonucleotides to destroy a NotI site downstream of the puroΔTK gene and introduce a NotI site upstream, and a FseI site downstream"}