PMC:1482699 / 34516-35006 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T20781","span":{"begin":0,"end":3},"obj":"NN"},{"id":"T20782","span":{"begin":23,"end":31},"obj":"VBN"},{"id":"T20783","span":{"begin":4,"end":7},"obj":"IN"},{"id":"T20784","span":{"begin":8,"end":18},"obj":"NN"},{"id":"T20785","span":{"begin":19,"end":22},"obj":"VBD"},{"id":"T20786","span":{"begin":32,"end":36},"obj":"IN"},{"id":"T20787","span":{"begin":37,"end":40},"obj":"CD"},{"id":"T20788","span":{"begin":41,"end":44},"obj":"CD"},{"id":"T20789","span":{"begin":40,"end":41},"obj":"SYM"},{"id":"T20790","span":{"begin":45,"end":47},"obj":"NN"},{"id":"T20791","span":{"begin":53,"end":58},"obj":"NNS"},{"id":"T20792","span":{"begin":48,"end":52},"obj":"NN"},{"id":"T20794","span":{"begin":62,"end":71},"obj":"VBG"},{"id":"T20795","span":{"begin":72,"end":76},"obj":"IN"},{"id":"T20796","span":{"begin":77,"end":87},"obj":"NN"},{"id":"T20797","span":{"begin":88,"end":89},"obj":"NN"},{"id":"T20798","span":{"begin":90,"end":91},"obj":"-LRB-"},{"id":"T20799","span":{"begin":91,"end":97},"obj":"NNP"},{"id":"T20800","span":{"begin":97,"end":98},"obj":"-RRB-"},{"id":"T20801","span":{"begin":99,"end":101},"obj":"IN"},{"id":"T20802","span":{"begin":102,"end":104},"obj":"CD"},{"id":"T20803","span":{"begin":104,"end":106},"obj":"NN"},{"id":"T20804","span":{"begin":107,"end":109},"obj":"IN"},{"id":"T20805","span":{"begin":110,"end":111},"obj":"DT"},{"id":"T20806","span":{"begin":112,"end":118},"obj":"NN"},{"id":"T20807","span":{"begin":119,"end":127},"obj":"VBN"},{"id":"T20808","span":{"begin":128,"end":130},"obj":"IN"},{"id":"T20809","span":{"begin":131,"end":135},"obj":"CD"},{"id":"T20810","span":{"begin":135,"end":136},"obj":"NN"},{"id":"T20811","span":{"begin":137,"end":141},"obj":"NN"},{"id":"T20812","span":{"begin":142,"end":143},"obj":"-LRB-"},{"id":"T20813","span":{"begin":143,"end":148},"obj":"NNP"},{"id":"T20814","span":{"begin":148,"end":149},"obj":"-RRB-"},{"id":"T20815","span":{"begin":149,"end":151},"obj":","},{"id":"T20816","span":{"begin":151,"end":155},"obj":"CD"},{"id":"T20817","span":{"begin":155,"end":156},"obj":"NN"},{"id":"T20818","span":{"begin":157,"end":162},"obj":"NN"},{"id":"T20819","span":{"begin":163,"end":165},"obj":"CD"},{"id":"T20820","span":{"begin":166,"end":167},"obj":"-LRB-"},{"id":"T20821","span":{"begin":167,"end":173},"obj":"NNP"},{"id":"T20822","span":{"begin":173,"end":174},"obj":"-RRB-"},{"id":"T20823","span":{"begin":175,"end":178},"obj":"CC"},{"id":"T20824","span":{"begin":179,"end":181},"obj":"NN"},{"id":"T20825","span":{"begin":186,"end":192},"obj":"NN"},{"id":"T20826","span":{"begin":182,"end":185},"obj":"NN"},{"id":"T20827","span":{"begin":193,"end":194},"obj":"-LRB-"},{"id":"T20828","span":{"begin":194,"end":201},"obj":"NNP"},{"id":"T20829","span":{"begin":201,"end":202},"obj":"-RRB-"},{"id":"T20830","span":{"begin":202,"end":203},"obj":"."},{"id":"T20831","span":{"begin":203,"end":321},"obj":"sentence"},{"id":"T20832","span":{"begin":204,"end":207},"obj":"DT"},{"id":"T20833","span":{"begin":208,"end":215},"obj":"NN"},{"id":"T20834","span":{"begin":238,"end":245},"obj":"VBN"},{"id":"T20835","span":{"begin":216,"end":218},"obj":"IN"},{"id":"T20836","span":{"begin":219,"end":223},"obj":"DT"},{"id":"T20837","span":{"begin":224,"end":233},"obj":"NN"},{"id":"T20838","span":{"begin":234,"end":237},"obj":"VBD"},{"id":"T20839","span":{"begin":246,"end":247},"obj":"-LRB-"},{"id":"T20840","span":{"begin":247,"end":248},"obj":"CD"},{"id":"T20841","span":{"begin":248,"end":249},"obj":"SYM"},{"id":"T20842","span":{"begin":249,"end":251},"obj":"CD"},{"id":"T20843","span":{"begin":251,"end":252},"obj":"-RRB-"},{"id":"T20844","span":{"begin":253,"end":255},"obj":"IN"},{"id":"T20845","span":{"begin":256,"end":263},"obj":"JJ"},{"id":"T20846","span":{"begin":264,"end":267},"obj":"NN"},{"id":"T20847","span":{"begin":268,"end":271},"obj":"CC"},{"id":"T20848","span":{"begin":272,"end":276},"obj":"VBN"},{"id":"T20849","span":{"begin":277,"end":280},"obj":"IN"},{"id":"T20850","span":{"begin":281,"end":291},"obj":"NN"},{"id":"T20851","span":{"begin":292,"end":299},"obj":"IN"},{"id":"T20852","span":{"begin":300,"end":307},"obj":"JJ"},{"id":"T20853","span":{"begin":308,"end":320},"obj":"NN"},{"id":"T20854","span":{"begin":320,"end":321},"obj":"."},{"id":"T20855","span":{"begin":321,"end":415},"obj":"sentence"},{"id":"T20856","span":{"begin":322,"end":336},"obj":"NN"},{"id":"T20857","span":{"begin":337,"end":347},"obj":"NN"},{"id":"T20858","span":{"begin":352,"end":361},"obj":"VBN"},{"id":"T20859","span":{"begin":348,"end":351},"obj":"VBD"},{"id":"T20860","span":{"begin":362,"end":367},"obj":"VBG"},{"id":"T20861","span":{"begin":368,"end":376},"obj":"JJ"},{"id":"T20862","span":{"begin":405,"end":414},"obj":"NNS"},{"id":"T20863","span":{"begin":377,"end":380},"obj":"NN"},{"id":"T20864","span":{"begin":381,"end":384},"obj":"CC"},{"id":"T20865","span":{"begin":385,"end":388},"obj":"NN"},{"id":"T20866","span":{"begin":389,"end":404},"obj":"NN"},{"id":"T20867","span":{"begin":414,"end":415},"obj":"."},{"id":"T20868","span":{"begin":415,"end":490},"obj":"sentence"},{"id":"T20869","span":{"begin":416,"end":424},"obj":"NN"},{"id":"T20870","span":{"begin":425,"end":435},"obj":"NNS"},{"id":"T20871","span":{"begin":456,"end":462},"obj":"VBN"},{"id":"T20872","span":{"begin":436,"end":439},"obj":"IN"},{"id":"T20873","span":{"begin":440,"end":444},"obj":"DT"},{"id":"T20874","span":{"begin":445,"end":451},"obj":"NN"},{"id":"T20875","span":{"begin":452,"end":455},"obj":"VBP"},{"id":"T20876","span":{"begin":463,"end":465},"obj":"IN"},{"id":"T20877","span":{"begin":466,"end":476},"obj":"JJ"},{"id":"T20878","span":{"begin":482,"end":483},"obj":"CD"},{"id":"T20879","span":{"begin":477,"end":481},"obj":"NN"},{"id":"T20880","span":{"begin":484,"end":487},"obj":"CC"},{"id":"T20881","span":{"begin":488,"end":489},"obj":"CD"},{"id":"T20882","span":{"begin":489,"end":490},"obj":"."},{"id":"T20793","span":{"begin":59,"end":61},"obj":"IN"}],"relations":[{"id":"R6392","pred":"nsubjpass","subj":"T20781","obj":"T20782"},{"id":"R6393","pred":"prep","subj":"T20783","obj":"T20781"},{"id":"R6394","pred":"pobj","subj":"T20784","obj":"T20783"},{"id":"R6395","pred":"auxpass","subj":"T20785","obj":"T20782"},{"id":"R6396","pred":"prep","subj":"T20786","obj":"T20782"},{"id":"R6397","pred":"quantmod","subj":"T20787","obj":"T20788"},{"id":"R6398","pred":"nummod","subj":"T20788","obj":"T20790"},{"id":"R6399","pred":"punct","subj":"T20789","obj":"T20788"},{"id":"R6400","pred":"compound","subj":"T20790","obj":"T20791"},{"id":"R6401","pred":"pobj","subj":"T20791","obj":"T20786"},{"id":"R6402","pred":"compound","subj":"T20792","obj":"T20791"},{"id":"R6403","pred":"prep","subj":"T20793","obj":"T20782"},{"id":"R6404","pred":"pcomp","subj":"T20794","obj":"T20793"},{"id":"R6405","pred":"prep","subj":"T20795","obj":"T20794"},{"id":"R6406","pred":"compound","subj":"T20796","obj":"T20797"},{"id":"R6407","pred":"pobj","subj":"T20797","obj":"T20795"},{"id":"R6408","pred":"punct","subj":"T20798","obj":"T20799"},{"id":"R6409","pred":"parataxis","subj":"T20799","obj":"T20797"},{"id":"R6410","pred":"punct","subj":"T20800","obj":"T20799"},{"id":"R6411","pred":"prep","subj":"T20801","obj":"T20794"},{"id":"R6412","pred":"nummod","subj":"T20802","obj":"T20803"},{"id":"R6413","pred":"pobj","subj":"T20803","obj":"T20801"},{"id":"R6414","pred":"prep","subj":"T20804","obj":"T20794"},{"id":"R6415","pred":"det","subj":"T20805","obj":"T20806"},{"id":"R6416","pred":"pobj","subj":"T20806","obj":"T20804"},{"id":"R6417","pred":"acl","subj":"T20807","obj":"T20806"},{"id":"R6418","pred":"prep","subj":"T20808","obj":"T20807"},{"id":"R6419","pred":"nummod","subj":"T20809","obj":"T20810"},{"id":"R6420","pred":"compound","subj":"T20810","obj":"T20811"},{"id":"R6421","pred":"pobj","subj":"T20811","obj":"T20808"},{"id":"R6422","pred":"punct","subj":"T20812","obj":"T20813"},{"id":"R6423","pred":"parataxis","subj":"T20813","obj":"T20811"},{"id":"R6424","pred":"punct","subj":"T20814","obj":"T20813"},{"id":"R6425","pred":"punct","subj":"T20815","obj":"T20811"},{"id":"R6426","pred":"nummod","subj":"T20816","obj":"T20817"},{"id":"R6427","pred":"compound","subj":"T20817","obj":"T20818"},{"id":"R6428","pred":"conj","subj":"T20818","obj":"T20811"},{"id":"R6429","pred":"nummod","subj":"T20819","obj":"T20818"},{"id":"R6430","pred":"punct","subj":"T20820","obj":"T20821"},{"id":"R6431","pred":"parataxis","subj":"T20821","obj":"T20818"},{"id":"R6432","pred":"punct","subj":"T20822","obj":"T20821"},{"id":"R6433","pred":"cc","subj":"T20823","obj":"T20818"},{"id":"R6434","pred":"compound","subj":"T20824","obj":"T20825"},{"id":"R6435","pred":"conj","subj":"T20825","obj":"T20818"},{"id":"R6436","pred":"compound","subj":"T20826","obj":"T20825"},{"id":"R6437","pred":"punct","subj":"T20827","obj":"T20828"},{"id":"R6438","pred":"parataxis","subj":"T20828","obj":"T20825"},{"id":"R6439","pred":"punct","subj":"T20829","obj":"T20828"},{"id":"R6440","pred":"punct","subj":"T20830","obj":"T20782"},{"id":"R6441","pred":"det","subj":"T20832","obj":"T20833"},{"id":"R6442","pred":"nsubjpass","subj":"T20833","obj":"T20834"},{"id":"R6443","pred":"prep","subj":"T20835","obj":"T20833"},{"id":"R6444","pred":"det","subj":"T20836","obj":"T20837"},{"id":"R6445","pred":"pobj","subj":"T20837","obj":"T20835"},{"id":"R6446","pred":"auxpass","subj":"T20838","obj":"T20834"},{"id":"R6447","pred":"punct","subj":"T20839","obj":"T20840"},{"id":"R6448","pred":"parataxis","subj":"T20840","obj":"T20834"},{"id":"R6449","pred":"punct","subj":"T20841","obj":"T20842"},{"id":"R6450","pred":"prep","subj":"T20842","obj":"T20840"},{"id":"R6451","pred":"punct","subj":"T20843","obj":"T20840"},{"id":"R6452","pred":"prep","subj":"T20844","obj":"T20834"},{"id":"R6453","pred":"amod","subj":"T20845","obj":"T20846"},{"id":"R6454","pred":"pobj","subj":"T20846","obj":"T20844"},{"id":"R6455","pred":"cc","subj":"T20847","obj":"T20834"},{"id":"R6456","pred":"conj","subj":"T20848","obj":"T20834"},{"id":"R6457","pred":"prep","subj":"T20849","obj":"T20848"},{"id":"R6458","pred":"pobj","subj":"T20850","obj":"T20849"},{"id":"R6459","pred":"prep","subj":"T20851","obj":"T20848"},{"id":"R6460","pred":"amod","subj":"T20852","obj":"T20853"},{"id":"R6461","pred":"pobj","subj":"T20853","obj":"T20851"},{"id":"R6462","pred":"punct","subj":"T20854","obj":"T20834"},{"id":"R6463","pred":"compound","subj":"T20856","obj":"T20857"},{"id":"R6464","pred":"nsubjpass","subj":"T20857","obj":"T20858"},{"id":"R6465","pred":"auxpass","subj":"T20859","obj":"T20858"},{"id":"R6466","pred":"advcl","subj":"T20860","obj":"T20858"},{"id":"R6467","pred":"amod","subj":"T20861","obj":"T20862"},{"id":"R6468","pred":"dobj","subj":"T20862","obj":"T20860"},{"id":"R6469","pred":"nmod","subj":"T20863","obj":"T20862"},{"id":"R6470","pred":"cc","subj":"T20864","obj":"T20863"},{"id":"R6471","pred":"compound","subj":"T20865","obj":"T20866"},{"id":"R6472","pred":"conj","subj":"T20866","obj":"T20863"},{"id":"R6473","pred":"punct","subj":"T20867","obj":"T20858"},{"id":"R6474","pred":"compound","subj":"T20869","obj":"T20870"},{"id":"R6475","pred":"nsubjpass","subj":"T20870","obj":"T20871"},{"id":"R6476","pred":"prep","subj":"T20872","obj":"T20870"},{"id":"R6477","pred":"det","subj":"T20873","obj":"T20874"},{"id":"R6478","pred":"pobj","subj":"T20874","obj":"T20872"},{"id":"R6479","pred":"auxpass","subj":"T20875","obj":"T20871"},{"id":"R6480","pred":"prep","subj":"T20876","obj":"T20871"},{"id":"R6481","pred":"amod","subj":"T20877","obj":"T20878"},{"id":"R6482","pred":"pobj","subj":"T20878","obj":"T20876"},{"id":"R6483","pred":"nmod","subj":"T20879","obj":"T20878"},{"id":"R6484","pred":"cc","subj":"T20880","obj":"T20878"},{"id":"R6485","pred":"conj","subj":"T20881","obj":"T20878"},{"id":"R6486","pred":"punct","subj":"T20882","obj":"T20871"}],"text":"DNA for genotyping was isolated from 1.0–2.0 mm tail clips by digesting with Proteinase K (Fisher) at 55°C in a buffer composed of 0.45% NP40 (Sigma), 0.45% Tween 20 (Fisher) and 1X PCR buffer (Promega). The product of this digestion was diluted (1:10) in sterile H2O and used for genotyping without further purification. Microsatellite genotyping was performed using standard PCR and gel electrophoresis protocols. Reaction conditions for each marker are listed in Additional File 1 and 2."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T20749","span":{"begin":0,"end":3},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T20750","span":{"begin":48,"end":52},"obj":"UBERON:0002415"},{"id":"T20751","span":{"begin":77,"end":87},"obj":"GO_EXT:0008233"},{"id":"T20752","span":{"begin":112,"end":118},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T20753","span":{"begin":137,"end":141},"obj":"CHEBI:63016"},{"id":"T20754","span":{"begin":157,"end":165},"obj":"CHEBI:53424"},{"id":"T20755","span":{"begin":186,"end":192},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T20756","span":{"begin":264,"end":267},"obj":"CHEBI:15377"},{"id":"T20757","span":{"begin":322,"end":336},"obj":"SO_EXT:microsatellite_unit_or_region"},{"id":"T20758","span":{"begin":445,"end":451},"obj":"CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag"}],"text":"DNA for genotyping was isolated from 1.0–2.0 mm tail clips by digesting with Proteinase K (Fisher) at 55°C in a buffer composed of 0.45% NP40 (Sigma), 0.45% Tween 20 (Fisher) and 1X PCR buffer (Promega). The product of this digestion was diluted (1:10) in sterile H2O and used for genotyping without further purification. Microsatellite genotyping was performed using standard PCR and gel electrophoresis protocols. Reaction conditions for each marker are listed in Additional File 1 and 2."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T20731","span":{"begin":48,"end":52},"obj":"UBERON:0002415"},{"id":"T20732","span":{"begin":137,"end":141},"obj":"CHEBI:63016"},{"id":"T20733","span":{"begin":157,"end":165},"obj":"CHEBI:53424"},{"id":"T20734","span":{"begin":264,"end":267},"obj":"CHEBI:15377"}],"text":"DNA for genotyping was isolated from 1.0–2.0 mm tail clips by digesting with Proteinase K (Fisher) at 55°C in a buffer composed of 0.45% NP40 (Sigma), 0.45% Tween 20 (Fisher) and 1X PCR buffer (Promega). The product of this digestion was diluted (1:10) in sterile H2O and used for genotyping without further purification. Microsatellite genotyping was performed using standard PCR and gel electrophoresis protocols. Reaction conditions for each marker are listed in Additional File 1 and 2."}