Id |
Subject |
Object |
Predicate |
Lexical cue |
T20781 |
0-3 |
NN |
denotes |
DNA |
T20782 |
23-31 |
VBN |
denotes |
isolated |
T20783 |
4-7 |
IN |
denotes |
for |
T20784 |
8-18 |
NN |
denotes |
genotyping |
T20785 |
19-22 |
VBD |
denotes |
was |
T20786 |
32-36 |
IN |
denotes |
from |
T20787 |
37-40 |
CD |
denotes |
1.0 |
T20788 |
41-44 |
CD |
denotes |
2.0 |
T20789 |
40-41 |
SYM |
denotes |
– |
T20790 |
45-47 |
NN |
denotes |
mm |
T20791 |
53-58 |
NNS |
denotes |
clips |
T20792 |
48-52 |
NN |
denotes |
tail |
T20794 |
62-71 |
VBG |
denotes |
digesting |
T20795 |
72-76 |
IN |
denotes |
with |
T20796 |
77-87 |
NN |
denotes |
Proteinase |
T20797 |
88-89 |
NN |
denotes |
K |
T20798 |
90-91 |
-LRB- |
denotes |
( |
T20799 |
91-97 |
NNP |
denotes |
Fisher |
T20800 |
97-98 |
-RRB- |
denotes |
) |
T20801 |
99-101 |
IN |
denotes |
at |
T20802 |
102-104 |
CD |
denotes |
55 |
T20803 |
104-106 |
NN |
denotes |
°C |
T20804 |
107-109 |
IN |
denotes |
in |
T20805 |
110-111 |
DT |
denotes |
a |
T20806 |
112-118 |
NN |
denotes |
buffer |
T20807 |
119-127 |
VBN |
denotes |
composed |
T20808 |
128-130 |
IN |
denotes |
of |
T20809 |
131-135 |
CD |
denotes |
0.45 |
T20810 |
135-136 |
NN |
denotes |
% |
T20811 |
137-141 |
NN |
denotes |
NP40 |
T20812 |
142-143 |
-LRB- |
denotes |
( |
T20813 |
143-148 |
NNP |
denotes |
Sigma |
T20814 |
148-149 |
-RRB- |
denotes |
) |
T20815 |
149-151 |
, |
denotes |
, |
T20816 |
151-155 |
CD |
denotes |
0.45 |
T20817 |
155-156 |
NN |
denotes |
% |
T20818 |
157-162 |
NN |
denotes |
Tween |
T20819 |
163-165 |
CD |
denotes |
20 |
T20820 |
166-167 |
-LRB- |
denotes |
( |
T20821 |
167-173 |
NNP |
denotes |
Fisher |
T20822 |
173-174 |
-RRB- |
denotes |
) |
T20823 |
175-178 |
CC |
denotes |
and |
T20824 |
179-181 |
NN |
denotes |
1X |
T20825 |
186-192 |
NN |
denotes |
buffer |
T20826 |
182-185 |
NN |
denotes |
PCR |
T20827 |
193-194 |
-LRB- |
denotes |
( |
T20828 |
194-201 |
NNP |
denotes |
Promega |
T20829 |
201-202 |
-RRB- |
denotes |
) |
T20830 |
202-203 |
. |
denotes |
. |
T20831 |
203-321 |
sentence |
denotes |
The product of this digestion was diluted (1:10) in sterile H2O and used for genotyping without further purification. |
T20832 |
204-207 |
DT |
denotes |
The |
T20833 |
208-215 |
NN |
denotes |
product |
T20834 |
238-245 |
VBN |
denotes |
diluted |
T20835 |
216-218 |
IN |
denotes |
of |
T20836 |
219-223 |
DT |
denotes |
this |
T20837 |
224-233 |
NN |
denotes |
digestion |
T20838 |
234-237 |
VBD |
denotes |
was |
T20839 |
246-247 |
-LRB- |
denotes |
( |
T20840 |
247-248 |
CD |
denotes |
1 |
T20841 |
248-249 |
SYM |
denotes |
: |
T20842 |
249-251 |
CD |
denotes |
10 |
T20843 |
251-252 |
-RRB- |
denotes |
) |
T20844 |
253-255 |
IN |
denotes |
in |
T20845 |
256-263 |
JJ |
denotes |
sterile |
T20846 |
264-267 |
NN |
denotes |
H2O |
T20847 |
268-271 |
CC |
denotes |
and |
T20848 |
272-276 |
VBN |
denotes |
used |
T20849 |
277-280 |
IN |
denotes |
for |
T20850 |
281-291 |
NN |
denotes |
genotyping |
T20851 |
292-299 |
IN |
denotes |
without |
T20852 |
300-307 |
JJ |
denotes |
further |
T20853 |
308-320 |
NN |
denotes |
purification |
T20854 |
320-321 |
. |
denotes |
. |
T20855 |
321-415 |
sentence |
denotes |
Microsatellite genotyping was performed using standard PCR and gel electrophoresis protocols. |
T20856 |
322-336 |
NN |
denotes |
Microsatellite |
T20857 |
337-347 |
NN |
denotes |
genotyping |
T20858 |
352-361 |
VBN |
denotes |
performed |
T20859 |
348-351 |
VBD |
denotes |
was |
T20860 |
362-367 |
VBG |
denotes |
using |
T20861 |
368-376 |
JJ |
denotes |
standard |
T20862 |
405-414 |
NNS |
denotes |
protocols |
T20863 |
377-380 |
NN |
denotes |
PCR |
T20864 |
381-384 |
CC |
denotes |
and |
T20865 |
385-388 |
NN |
denotes |
gel |
T20866 |
389-404 |
NN |
denotes |
electrophoresis |
T20867 |
414-415 |
. |
denotes |
. |
T20868 |
415-490 |
sentence |
denotes |
Reaction conditions for each marker are listed in Additional File 1 and 2. |
T20869 |
416-424 |
NN |
denotes |
Reaction |
T20870 |
425-435 |
NNS |
denotes |
conditions |
T20871 |
456-462 |
VBN |
denotes |
listed |
T20872 |
436-439 |
IN |
denotes |
for |
T20873 |
440-444 |
DT |
denotes |
each |
T20874 |
445-451 |
NN |
denotes |
marker |
T20875 |
452-455 |
VBP |
denotes |
are |
T20876 |
463-465 |
IN |
denotes |
in |
T20877 |
466-476 |
JJ |
denotes |
Additional |
T20878 |
482-483 |
CD |
denotes |
1 |
T20879 |
477-481 |
NN |
denotes |
File |
T20880 |
484-487 |
CC |
denotes |
and |
T20881 |
488-489 |
CD |
denotes |
2 |
T20882 |
489-490 |
. |
denotes |
. |
T20793 |
59-61 |
IN |
denotes |
by |
R6392 |
T20781 |
T20782 |
nsubjpass |
DNA,isolated |
R6393 |
T20783 |
T20781 |
prep |
for,DNA |
R6394 |
T20784 |
T20783 |
pobj |
genotyping,for |
R6395 |
T20785 |
T20782 |
auxpass |
was,isolated |
R6396 |
T20786 |
T20782 |
prep |
from,isolated |
R6397 |
T20787 |
T20788 |
quantmod |
1.0,2.0 |
R6398 |
T20788 |
T20790 |
nummod |
2.0,mm |
R6399 |
T20789 |
T20788 |
punct |
–,2.0 |
R6400 |
T20790 |
T20791 |
compound |
mm,clips |
R6401 |
T20791 |
T20786 |
pobj |
clips,from |
R6402 |
T20792 |
T20791 |
compound |
tail,clips |
R6403 |
T20793 |
T20782 |
prep |
by,isolated |
R6404 |
T20794 |
T20793 |
pcomp |
digesting,by |
R6405 |
T20795 |
T20794 |
prep |
with,digesting |
R6406 |
T20796 |
T20797 |
compound |
Proteinase,K |
R6407 |
T20797 |
T20795 |
pobj |
K,with |
R6408 |
T20798 |
T20799 |
punct |
(,Fisher |
R6409 |
T20799 |
T20797 |
parataxis |
Fisher,K |
R6410 |
T20800 |
T20799 |
punct |
),Fisher |
R6411 |
T20801 |
T20794 |
prep |
at,digesting |
R6412 |
T20802 |
T20803 |
nummod |
55,°C |
R6413 |
T20803 |
T20801 |
pobj |
°C,at |
R6414 |
T20804 |
T20794 |
prep |
in,digesting |
R6415 |
T20805 |
T20806 |
det |
a,buffer |
R6416 |
T20806 |
T20804 |
pobj |
buffer,in |
R6417 |
T20807 |
T20806 |
acl |
composed,buffer |
R6418 |
T20808 |
T20807 |
prep |
of,composed |
R6419 |
T20809 |
T20810 |
nummod |
0.45,% |
R6420 |
T20810 |
T20811 |
compound |
%,NP40 |
R6421 |
T20811 |
T20808 |
pobj |
NP40,of |
R6422 |
T20812 |
T20813 |
punct |
(,Sigma |
R6423 |
T20813 |
T20811 |
parataxis |
Sigma,NP40 |
R6424 |
T20814 |
T20813 |
punct |
),Sigma |
R6425 |
T20815 |
T20811 |
punct |
", ",NP40 |
R6426 |
T20816 |
T20817 |
nummod |
0.45,% |
R6427 |
T20817 |
T20818 |
compound |
%,Tween |
R6428 |
T20818 |
T20811 |
conj |
Tween,NP40 |
R6429 |
T20819 |
T20818 |
nummod |
20,Tween |
R6430 |
T20820 |
T20821 |
punct |
(,Fisher |
R6431 |
T20821 |
T20818 |
parataxis |
Fisher,Tween |
R6432 |
T20822 |
T20821 |
punct |
),Fisher |
R6433 |
T20823 |
T20818 |
cc |
and,Tween |
R6434 |
T20824 |
T20825 |
compound |
1X,buffer |
R6435 |
T20825 |
T20818 |
conj |
buffer,Tween |
R6436 |
T20826 |
T20825 |
compound |
PCR,buffer |
R6437 |
T20827 |
T20828 |
punct |
(,Promega |
R6438 |
T20828 |
T20825 |
parataxis |
Promega,buffer |
R6439 |
T20829 |
T20828 |
punct |
),Promega |
R6440 |
T20830 |
T20782 |
punct |
.,isolated |
R6441 |
T20832 |
T20833 |
det |
The,product |
R6442 |
T20833 |
T20834 |
nsubjpass |
product,diluted |
R6443 |
T20835 |
T20833 |
prep |
of,product |
R6444 |
T20836 |
T20837 |
det |
this,digestion |
R6445 |
T20837 |
T20835 |
pobj |
digestion,of |
R6446 |
T20838 |
T20834 |
auxpass |
was,diluted |
R6447 |
T20839 |
T20840 |
punct |
(,1 |
R6448 |
T20840 |
T20834 |
parataxis |
1,diluted |
R6449 |
T20841 |
T20842 |
punct |
:,10 |
R6450 |
T20842 |
T20840 |
prep |
10,1 |
R6451 |
T20843 |
T20840 |
punct |
),1 |
R6452 |
T20844 |
T20834 |
prep |
in,diluted |
R6453 |
T20845 |
T20846 |
amod |
sterile,H2O |
R6454 |
T20846 |
T20844 |
pobj |
H2O,in |
R6455 |
T20847 |
T20834 |
cc |
and,diluted |
R6456 |
T20848 |
T20834 |
conj |
used,diluted |
R6457 |
T20849 |
T20848 |
prep |
for,used |
R6458 |
T20850 |
T20849 |
pobj |
genotyping,for |
R6459 |
T20851 |
T20848 |
prep |
without,used |
R6460 |
T20852 |
T20853 |
amod |
further,purification |
R6461 |
T20853 |
T20851 |
pobj |
purification,without |
R6462 |
T20854 |
T20834 |
punct |
.,diluted |
R6463 |
T20856 |
T20857 |
compound |
Microsatellite,genotyping |
R6464 |
T20857 |
T20858 |
nsubjpass |
genotyping,performed |
R6465 |
T20859 |
T20858 |
auxpass |
was,performed |
R6466 |
T20860 |
T20858 |
advcl |
using,performed |
R6467 |
T20861 |
T20862 |
amod |
standard,protocols |
R6468 |
T20862 |
T20860 |
dobj |
protocols,using |
R6469 |
T20863 |
T20862 |
nmod |
PCR,protocols |
R6470 |
T20864 |
T20863 |
cc |
and,PCR |
R6471 |
T20865 |
T20866 |
compound |
gel,electrophoresis |
R6472 |
T20866 |
T20863 |
conj |
electrophoresis,PCR |
R6473 |
T20867 |
T20858 |
punct |
.,performed |
R6474 |
T20869 |
T20870 |
compound |
Reaction,conditions |
R6475 |
T20870 |
T20871 |
nsubjpass |
conditions,listed |
R6476 |
T20872 |
T20870 |
prep |
for,conditions |
R6477 |
T20873 |
T20874 |
det |
each,marker |
R6478 |
T20874 |
T20872 |
pobj |
marker,for |
R6479 |
T20875 |
T20871 |
auxpass |
are,listed |
R6480 |
T20876 |
T20871 |
prep |
in,listed |
R6481 |
T20877 |
T20878 |
amod |
Additional,1 |
R6482 |
T20878 |
T20876 |
pobj |
1,in |
R6483 |
T20879 |
T20878 |
nmod |
File,1 |
R6484 |
T20880 |
T20878 |
cc |
and,1 |
R6485 |
T20881 |
T20878 |
conj |
2,1 |
R6486 |
T20882 |
T20871 |
punct |
.,listed |