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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1481596","sourcedb":"PMC","sourceid":"1481596","source_url":"https://www.ncbi.nlm.nih.gov/pmc/1481596","text":"Data on protein complexes are collected from the study of individual systems, and more recently through high-throughput experiments, such as yeast two-hybrid (Y2H) [1,2] and tandem affinity purification followed by mass spectrometry (TAP/MS) [3,4]. The TAP/MS approach helps pinpoint proteins that interact with a tagged bait protein, either directly or indirectly, and are thus suited to identify multi-protein complexes. In fact, several research groups have systematically applied TAP/MS technology to study protein complexes involved in different signaling pathways [5].","tracks":[{"project":"2_test","denotations":[{"id":"16722537-10688190-1694473","span":{"begin":165,"end":166},"obj":"10688190"},{"id":"16722537-11283351-1694474","span":{"begin":167,"end":168},"obj":"11283351"},{"id":"16722537-11805837-1694475","span":{"begin":243,"end":244},"obj":"11805837"},{"id":"16722537-11805826-1694476","span":{"begin":245,"end":246},"obj":"11805826"},{"id":"16722537-14743216-1694477","span":{"begin":571,"end":572},"obj":"14743216"}],"attributes":[{"subj":"16722537-10688190-1694473","pred":"source","obj":"2_test"},{"subj":"16722537-11283351-1694474","pred":"source","obj":"2_test"},{"subj":"16722537-11805837-1694475","pred":"source","obj":"2_test"},{"subj":"16722537-11805826-1694476","pred":"source","obj":"2_test"},{"subj":"16722537-14743216-1694477","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#e6ec93","default":true}]}]}}