PMC:1481596 / 2368-2942 JSONTXT

{"project":"2_test","denotations":[{"id":"16722537-10688190-1694473","span":{"begin":165,"end":166},"obj":"10688190"},{"id":"16722537-11283351-1694474","span":{"begin":167,"end":168},"obj":"11283351"},{"id":"16722537-11805837-1694475","span":{"begin":243,"end":244},"obj":"11805837"},{"id":"16722537-11805826-1694476","span":{"begin":245,"end":246},"obj":"11805826"},{"id":"16722537-14743216-1694477","span":{"begin":571,"end":572},"obj":"14743216"}],"text":"Data on protein complexes are collected from the study of individual systems, and more recently through high-throughput experiments, such as yeast two-hybrid (Y2H) [1,2] and tandem affinity purification followed by mass spectrometry (TAP/MS) [3,4]. The TAP/MS approach helps pinpoint proteins that interact with a tagged bait protein, either directly or indirectly, and are thus suited to identify multi-protein complexes. In fact, several research groups have systematically applied TAP/MS technology to study protein complexes involved in different signaling pathways [5]."}