PMC:1472690 / 8949-10204 JSONTXT

{"project":"pmc-enju-pas","denotations":[{"id":"T21837","span":{"begin":1253,"end":1254},"obj":"-RRB-"},{"id":"T21836","span":{"begin":1252,"end":1253},"obj":"CD"},{"id":"T21835","span":{"begin":1250,"end":1251},"obj":"SYM"},{"id":"T21834","span":{"begin":1248,"end":1249},"obj":"NN"},{"id":"T21833","span":{"begin":1247,"end":1248},"obj":"-LRB-"},{"id":"T21832","span":{"begin":1239,"end":1246},"obj":"NN"},{"id":"T21831","span":{"begin":1232,"end":1238},"obj":"NN"},{"id":"T21830","span":{"begin":1229,"end":1231},"obj":"TO"},{"id":"T21829","span":{"begin":1221,"end":1228},"obj":"NN"},{"id":"T21828","span":{"begin":1216,"end":1220},"obj":"IN"},{"id":"T21827","span":{"begin":1210,"end":1215},"obj":"VB"},{"id":"T21826","span":{"begin":1206,"end":1209},"obj":"VB"},{"id":"T21825","span":{"begin":1204,"end":1205},"obj":"-RRB-"},{"id":"T21824","span":{"begin":1200,"end":1204},"obj":"CD"},{"id":"T21823","span":{"begin":1198,"end":1199},"obj":"SYM"},{"id":"T21822","span":{"begin":1196,"end":1197},"obj":"NN"},{"id":"T21821","span":{"begin":1194,"end":1195},"obj":"SYM"},{"id":"T21820","span":{"begin":1192,"end":1193},"obj":"SYM"},{"id":"T21819","span":{"begin":1191,"end":1192},"obj":"-LRB-"},{"id":"T21818","span":{"begin":1177,"end":1190},"obj":"NN"},{"id":"T21817","span":{"begin":1173,"end":1176},"obj":"CC"},{"id":"T21816","span":{"begin":1169,"end":1172},"obj":"NN"},{"id":"T21815","span":{"begin":1166,"end":1168},"obj":"VB"},{"id":"T21814","span":{"begin":1163,"end":1165},"obj":"TO"},{"id":"T21813","span":{"begin":1157,"end":1162},"obj":"JJ"},{"id":"T21812","span":{"begin":1153,"end":1156},"obj":"VB"},{"id":"T21811","span":{"begin":1148,"end":1152},"obj":"VB"},{"id":"T21810","span":{"begin":1141,"end":1147},"obj":"NN"},{"id":"T21809","span":{"begin":1133,"end":1140},"obj":"NN"},{"id":"T21808","span":{"begin":1126,"end":1132},"obj":"NN"},{"id":"T21807","span":{"begin":1123,"end":1124},"obj":"-RRB-"},{"id":"T21806","span":{"begin":1118,"end":1123},"obj":"NN"},{"id":"T21805","span":{"begin":1108,"end":1117},"obj":"NN"},{"id":"T21804","span":{"begin":1107,"end":1108},"obj":"-LRB-"},{"id":"T21803","span":{"begin":1101,"end":1106},"obj":"NN"},{"id":"T21802","span":{"begin":1089,"end":1100},"obj":"JJ"},{"id":"T21801","span":{"begin":1085,"end":1088},"obj":"DT"},{"id":"T21800","span":{"begin":1081,"end":1084},"obj":"CC"},{"id":"T21799","span":{"begin":1071,"end":1080},"obj":"NN"},{"id":"T21798","span":{"begin":1066,"end":1070},"obj":"DT"},{"id":"T21797","span":{"begin":1063,"end":1065},"obj":"IN"},{"id":"T21796","span":{"begin":1048,"end":1062},"obj":"NN"},{"id":"T21795","span":{"begin":1035,"end":1047},"obj":"JJ"},{"id":"T21794","span":{"begin":1032,"end":1034},"obj":"IN"},{"id":"T21793","span":{"begin":1027,"end":1031},"obj":"RB"},{"id":"T21792","span":{"begin":1024,"end":1026},"obj":"RB"},{"id":"T21791","span":{"begin":1022,"end":1023},"obj":"-RRB-"},{"id":"T21790","span":{"begin":1020,"end":1022},"obj":"NN"},{"id":"T21789","span":{"begin":1016,"end":1019},"obj":"CD"},{"id":"T21788","span":{"begin":1015,"end":1016},"obj":"-LRB-"},{"id":"T21787","span":{"begin":1005,"end":1014},"obj":"NN"},{"id":"T21786","span":{"begin":998,"end":1004},"obj":"NN"},{"id":"T21785","span":{"begin":995,"end":997},"obj":"CC"},{"id":"T21784","span":{"begin":993,"end":994},"obj":"-RRB-"},{"id":"T21783","span":{"begin":991,"end":993},"obj":"NN"},{"id":"T21782","span":{"begin":987,"end":990},"obj":"CD"},{"id":"T21781","span":{"begin":983,"end":986},"obj":"CC"},{"id":"T21780","span":{"begin":981,"end":982},"obj":"CD"},{"id":"T21779","span":{"begin":980,"end":981},"obj":"-LRB-"},{"id":"T21778","span":{"begin":977,"end":979},"obj":"NN"},{"id":"T21777","span":{"begin":967,"end":976},"obj":"VB"},{"id":"T21776","span":{"begin":958,"end":966},"obj":"NN"},{"id":"T21775","span":{"begin":950,"end":957},"obj":"NN"},{"id":"T21774","span":{"begin":941,"end":949},"obj":"NN"},{"id":"T21773","span":{"begin":938,"end":940},"obj":"IN"},{"id":"T21772","span":{"begin":923,"end":937},"obj":"NN"},{"id":"T21771","span":{"begin":913,"end":922},"obj":"JJ"},{"id":"T21770","span":{"begin":906,"end":911},"obj":"VB"},{"id":"T21769","span":{"begin":903,"end":905},"obj":"VB"},{"id":"T21768","span":{"begin":892,"end":902},"obj":"NN"},{"id":"T21767","span":{"begin":877,"end":891},"obj":"JJ"},{"id":"T21766","span":{"begin":875,"end":876},"obj":"DT"},{"id":"T21765","span":{"begin":870,"end":874},"obj":"IN"},{"id":"T21764","span":{"begin":856,"end":869},"obj":"NN"},{"id":"T21763","span":{"begin":853,"end":855},"obj":"NNP"},{"id":"T21762","span":{"begin":847,"end":852},"obj":"NNP"},{"id":"T21761","span":{"begin":844,"end":846},"obj":"IN"},{"id":"T21760","span":{"begin":842,"end":843},"obj":"NN"},{"id":"T21759","span":{"begin":840,"end":841},"obj":"CD"},{"id":"T21758","span":{"begin":836,"end":839},"obj":"IN"},{"id":"T21757","span":{"begin":832,"end":835},"obj":"NN"},{"id":"T21756","span":{"begin":830,"end":831},"obj":"SYM"},{"id":"T21755","span":{"begin":827,"end":829},"obj":"NN"},{"id":"T21754","span":{"begin":823,"end":826},"obj":"CC"},{"id":"T21753","span":{"begin":816,"end":822},"obj":"NN"},{"id":"T21752","span":{"begin":814,"end":815},"obj":"SYM"},{"id":"T21751","span":{"begin":811,"end":813},"obj":"NN"},{"id":"T21750","span":{"begin":809,"end":810},"obj":"-COMMA-"},{"id":"T21749","span":{"begin":808,"end":809},"obj":"-RRB-"},{"id":"T21748","span":{"begin":806,"end":808},"obj":"NN"},{"id":"T21747","span":{"begin":802,"end":805},"obj":"CD"},{"id":"T21746","span":{"begin":801,"end":802},"obj":"-LRB-"},{"id":"T21745","span":{"begin":798,"end":800},"obj":"NN"},{"id":"T21744","span":{"begin":796,"end":797},"obj":"-COMMA-"},{"id":"T21743","span":{"begin":790,"end":796},"obj":"NN"},{"id":"T21742","span":{"begin":788,"end":789},"obj":"-COMMA-"},{"id":"T21741","span":{"begin":785,"end":788},"obj":"NN"},{"id":"T21740","span":{"begin":783,"end":784},"obj":"-COMMA-"},{"id":"T21739","span":{"begin":776,"end":783},"obj":"NN"},{"id":"T21738","span":{"begin":769,"end":775},"obj":"NN"},{"id":"T21737","span":{"begin":764,"end":768},"obj":"IN"},{"id":"T21736","span":{"begin":754,"end":763},"obj":"VB"},{"id":"T21735","span":{"begin":749,"end":753},"obj":"VB"},{"id":"T21734","span":{"begin":744,"end":748},"obj":"NN"},{"id":"T21733","span":{"begin":738,"end":743},"obj":"JJ"},{"id":"T21732","span":{"begin":727,"end":736},"obj":"NN"},{"id":"T21731","span":{"begin":724,"end":726},"obj":"CC"},{"id":"T21730","span":{"begin":722,"end":723},"obj":"-COMMA-"},{"id":"T21729","span":{"begin":720,"end":722},"obj":"NN"},{"id":"T21728","span":{"begin":718,"end":719},"obj":"-COMMA-"},{"id":"T21727","span":{"begin":712,"end":718},"obj":"NN"},{"id":"T21726","span":{"begin":709,"end":711},"obj":"TO"},{"id":"T21725","span":{"begin":700,"end":708},"obj":"VB"},{"id":"T21724","span":{"begin":698,"end":699},"obj":"-COMMA-"},{"id":"T21723","span":{"begin":692,"end":698},"obj":"NN"},{"id":"T21722","span":{"begin":690,"end":691},"obj":"SYM"},{"id":"T21721","span":{"begin":687,"end":689},"obj":"NN"},{"id":"T21720","span":{"begin":677,"end":686},"obj":"VB"},{"id":"T21719","span":{"begin":672,"end":676},"obj":"NN"},{"id":"T21718","span":{"begin":669,"end":671},"obj":"IN"},{"id":"T21717","span":{"begin":661,"end":668},"obj":"NN"},{"id":"T21716","span":{"begin":657,"end":660},"obj":"NN"},{"id":"T21715","span":{"begin":647,"end":656},"obj":"VB"},{"id":"T21714","span":{"begin":645,"end":646},"obj":"-COMMA-"},{"id":"T21713","span":{"begin":644,"end":645},"obj":"NN"},{"id":"T21712","span":{"begin":641,"end":642},"obj":"-RRB-"},{"id":"T21711","span":{"begin":640,"end":641},"obj":"CD"},{"id":"T21710","span":{"begin":638,"end":639},"obj":"SYM"},{"id":"T21709","span":{"begin":636,"end":637},"obj":"NN"},{"id":"T21708","span":{"begin":635,"end":636},"obj":"-LRB-"},{"id":"T21707","span":{"begin":629,"end":634},"obj":"VB"},{"id":"T21706","span":{"begin":626,"end":628},"obj":"VB"},{"id":"T21705","span":{"begin":615,"end":625},"obj":"NN"},{"id":"T21704","span":{"begin":600,"end":614},"obj":"JJ"},{"id":"T21703","span":{"begin":598,"end":599},"obj":"DT"},{"id":"T21702","span":{"begin":593,"end":597},"obj":"IN"},{"id":"T21701","span":{"begin":579,"end":592},"obj":"NN"},{"id":"T21700","span":{"begin":576,"end":578},"obj":"DT"},{"id":"T21699","span":{"begin":562,"end":574},"obj":"RB"},{"id":"T21698","span":{"begin":560,"end":561},"obj":"-COMMA-"},{"id":"T21697","span":{"begin":556,"end":560},"obj":"CD"},{"id":"T21696","span":{"begin":552,"end":555},"obj":"CC"},{"id":"T21695","span":{"begin":529,"end":551},"obj":"NN"},{"id":"T21694","span":{"begin":526,"end":528},"obj":"IN"},{"id":"T21693","span":{"begin":515,"end":525},"obj":"NN"},{"id":"T21692","span":{"begin":513,"end":514},"obj":"DT"},{"id":"T21691","span":{"begin":503,"end":512},"obj":"VB"},{"id":"T21690","span":{"begin":498,"end":502},"obj":"NN"},{"id":"T21689","span":{"begin":493,"end":497},"obj":"JJ"},{"id":"T21688","span":{"begin":489,"end":492},"obj":"DT"},{"id":"T21687","span":{"begin":484,"end":488},"obj":"IN"},{"id":"T21686","span":{"begin":478,"end":483},"obj":"NN"},{"id":"T21685","span":{"begin":475,"end":476},"obj":"-RRB-"},{"id":"T21684","span":{"begin":470,"end":475},"obj":"NN"},{"id":"T21683","span":{"begin":467,"end":469},"obj":"DT"},{"id":"T21682","span":{"begin":466,"end":467},"obj":"-LRB-"},{"id":"T21681","span":{"begin":460,"end":465},"obj":"NN"},{"id":"T21680","span":{"begin":455,"end":459},"obj":"JJ"},{"id":"T21679","span":{"begin":452,"end":454},"obj":"TO"},{"id":"T21678","span":{"begin":443,"end":451},"obj":"VB"},{"id":"T21677","span":{"begin":435,"end":442},"obj":"NN"},{"id":"T21676","span":{"begin":421,"end":434},"obj":"NN"},{"id":"T21675","span":{"begin":418,"end":420},"obj":"CC"},{"id":"T21674","span":{"begin":407,"end":417},"obj":"NN"},{"id":"T21673","span":{"begin":405,"end":406},"obj":"-COMMA-"},{"id":"T21672","span":{"begin":397,"end":405},"obj":"NN"},{"id":"T21671","span":{"begin":395,"end":396},"obj":"-COMMA-"},{"id":"T21670","span":{"begin":387,"end":395},"obj":"NN"},{"id":"T21669","span":{"begin":383,"end":386},"obj":"DT"},{"id":"T21668","span":{"begin":380,"end":382},"obj":"IN"},{"id":"T21667","span":{"begin":371,"end":379},"obj":"NN"},{"id":"T21666","span":{"begin":359,"end":370},"obj":"JJ"},{"id":"T21665","span":{"begin":350,"end":358},"obj":"VB"},{"id":"T21664","span":{"begin":345,"end":349},"obj":"NN"},{"id":"T21663","span":{"begin":339,"end":344},"obj":"JJ"},{"id":"T21662","span":{"begin":335,"end":338},"obj":"DT"},{"id":"T21661","span":{"begin":330,"end":334},"obj":"IN"},{"id":"T21660","span":{"begin":323,"end":329},"obj":"NN"},{"id":"T21659","span":{"begin":320,"end":321},"obj":"-RRB-"},{"id":"T21658","span":{"begin":319,"end":320},"obj":"CD"},{"id":"T21657","span":{"begin":313,"end":318},"obj":"JJ"},{"id":"T21656","span":{"begin":312,"end":313},"obj":"-LRB-"},{"id":"T21655","span":{"begin":302,"end":311},"obj":"NN"},{"id":"T21654","span":{"begin":297,"end":301},"obj":"NN"},{"id":"T21653","span":{"begin":292,"end":296},"obj":"NN"},{"id":"T21652","span":{"begin":288,"end":291},"obj":"CC"},{"id":"T21651","span":{"begin":283,"end":287},"obj":"NN"},{"id":"T21650","span":{"begin":281,"end":282},"obj":"-COMMA-"},{"id":"T21649","span":{"begin":277,"end":281},"obj":"NN"},{"id":"T21648","span":{"begin":275,"end":276},"obj":"-COMMA-"},{"id":"T21647","span":{"begin":271,"end":275},"obj":"NN"},{"id":"T21646","span":{"begin":267,"end":270},"obj":"DT"},{"id":"T21645","span":{"begin":264,"end":266},"obj":"TO"},{"id":"T21644","span":{"begin":250,"end":263},"obj":"VB"},{"id":"T21643","span":{"begin":244,"end":249},"obj":"NN"},{"id":"T21642","span":{"begin":228,"end":243},"obj":"NN"},{"id":"T21641","span":{"begin":218,"end":227},"obj":"NN"},{"id":"T21640","span":{"begin":215,"end":217},"obj":"CC"},{"id":"T21639","span":{"begin":208,"end":214},"obj":"NN"},{"id":"T21638","span":{"begin":206,"end":207},"obj":"-COMMA-"},{"id":"T21637","span":{"begin":202,"end":206},"obj":"NN"},{"id":"T21636","span":{"begin":200,"end":201},"obj":"-COMMA-"},{"id":"T21635","span":{"begin":196,"end":200},"obj":"NN"},{"id":"T21634","span":{"begin":184,"end":195},"obj":"JJ"},{"id":"T21633","span":{"begin":182,"end":183},"obj":"DT"},{"id":"T21632","span":{"begin":177,"end":181},"obj":"IN"},{"id":"T21631","span":{"begin":167,"end":176},"obj":"VB"},{"id":"T21630","span":{"begin":162,"end":166},"obj":"VB"},{"id":"T21629","span":{"begin":153,"end":161},"obj":"NN"},{"id":"T21628","span":{"begin":145,"end":152},"obj":"NNP"},{"id":"T21627","span":{"begin":139,"end":144},"obj":"NNP"},{"id":"T21626","span":{"begin":136,"end":138},"obj":"IN"},{"id":"T21625","span":{"begin":134,"end":135},"obj":"NN"},{"id":"T21624","span":{"begin":132,"end":133},"obj":"CD"},{"id":"T21623","span":{"begin":128,"end":131},"obj":"IN"},{"id":"T21622","span":{"begin":126,"end":127},"obj":"-RRB-"},{"id":"T21621","span":{"begin":124,"end":126},"obj":"NN"},{"id":"T21620","span":{"begin":120,"end":123},"obj":"CD"},{"id":"T21619","span":{"begin":116,"end":119},"obj":"CC"},{"id":"T21618","span":{"begin":114,"end":115},"obj":"CD"},{"id":"T21617","span":{"begin":113,"end":114},"obj":"-LRB-"},{"id":"T21616","span":{"begin":110,"end":112},"obj":"NN"},{"id":"T21615","span":{"begin":107,"end":109},"obj":"CC"},{"id":"T21614","span":{"begin":99,"end":106},"obj":"NN"},{"id":"T21613","span":{"begin":92,"end":98},"obj":"NN"},{"id":"T21612","span":{"begin":87,"end":91},"obj":"IN"},{"id":"T21611","span":{"begin":77,"end":86},"obj":"VB"},{"id":"T21610","span":{"begin":72,"end":76},"obj":"VB"},{"id":"T21609","span":{"begin":67,"end":71},"obj":"NN"},{"id":"T21608","span":{"begin":61,"end":66},"obj":"JJ"},{"id":"T21607","span":{"begin":53,"end":59},"obj":"NN"},{"id":"T21606","span":{"begin":49,"end":52},"obj":"CC"},{"id":"T21605","span":{"begin":44,"end":48},"obj":"NN"},{"id":"T21604","span":{"begin":42,"end":43},"obj":"-COMMA-"},{"id":"T21603","span":{"begin":38,"end":42},"obj":"NN"},{"id":"T21602","span":{"begin":35,"end":37},"obj":"IN"},{"id":"T21601","span":{"begin":27,"end":34},"obj":"NN"},{"id":"T21600","span":{"begin":23,"end":26},"obj":"NN"},{"id":"T21599","span":{"begin":20,"end":22},"obj":"TO"},{"id":"T21598","span":{"begin":16,"end":19},"obj":"VB"},{"id":"T21597","span":{"begin":13,"end":15},"obj":"NN"},{"id":"T21596","span":{"begin":11,"end":12},"obj":"-COMMA-"},{"id":"T21595","span":{"begin":10,"end":11},"obj":"DT"}],"relations":[{"id":"R14768","pred":"arg1Of","subj":"T21597","obj":"T21595"},{"id":"R14769","pred":"arg1Of","subj":"T21597","obj":"T21596"},{"id":"R14770","pred":"arg1Of","subj":"T21597","obj":"T21598"},{"id":"R14771","pred":"arg1Of","subj":"T21598","obj":"T21599"},{"id":"R14772","pred":"arg2Of","subj":"T21601","obj":"T21599"},{"id":"R14773","pred":"arg1Of","subj":"T21601","obj":"T21600"},{"id":"R14774","pred":"arg1Of","subj":"T21601","obj":"T21602"},{"id":"R14775","pred":"arg1Of","subj":"T21603","obj":"T21604"},{"id":"R14776","pred":"arg1Of","subj":"T21604","obj":"T21606"},{"id":"R14777","pred":"arg2Of","subj":"T21605","obj":"T21604"},{"id":"R14778","pred":"arg2Of","subj":"T21606","obj":"T21602"},{"id":"R14779","pred":"arg2Of","subj":"T21607","obj":"T21606"},{"id":"R14780","pred":"arg1Of","subj":"T21609","obj":"T21608"},{"id":"R14781","pred":"arg1Of","subj":"T21609","obj":"T21610"},{"id":"R14782","pred":"arg2Of","subj":"T21609","obj":"T21611"},{"id":"R14783","pred":"arg2Of","subj":"T21611","obj":"T21610"},{"id":"R14784","pred":"arg1Of","subj":"T21611","obj":"T21612"},{"id":"R14785","pred":"arg1Of","subj":"T21611","obj":"T21615"},{"id":"R14786","pred":"arg2Of","subj":"T21614","obj":"T21612"},{"id":"R14787","pred":"arg1Of","subj":"T21614","obj":"T21613"},{"id":"R14788","pred":"arg1Of","subj":"T21616","obj":"T21617"},{"id":"R14789","pred":"arg1Of","subj":"T21616","obj":"T21623"},{"id":"R14790","pred":"arg1Of","subj":"T21616","obj":"T21630"},{"id":"R14791","pred":"arg2Of","subj":"T21616","obj":"T21631"},{"id":"R14792","pred":"arg1Of","subj":"T21618","obj":"T21619"},{"id":"R14793","pred":"arg2Of","subj":"T21620","obj":"T21619"},{"id":"R14794","pred":"arg2Of","subj":"T21621","obj":"T21617"},{"id":"R14795","pred":"arg1Of","subj":"T21621","obj":"T21618"},{"id":"R14796","pred":"arg1Of","subj":"T21621","obj":"T21620"},{"id":"R14797","pred":"arg3Of","subj":"T21622","obj":"T21617"},{"id":"R14798","pred":"arg2Of","subj":"T21625","obj":"T21623"},{"id":"R14799","pred":"arg1Of","subj":"T21625","obj":"T21624"},{"id":"R14800","pred":"arg1Of","subj":"T21625","obj":"T21626"},{"id":"R14801","pred":"arg1Of","subj":"T21628","obj":"T21627"},{"id":"R14802","pred":"arg2Of","subj":"T21629","obj":"T21626"},{"id":"R14803","pred":"arg1Of","subj":"T21629","obj":"T21628"},{"id":"R14804","pred":"arg2Of","subj":"T21631","obj":"T21615"},{"id":"R14805","pred":"arg2Of","subj":"T21631","obj":"T21630"},{"id":"R14806","pred":"arg1Of","subj":"T21631","obj":"T21632"},{"id":"R14807","pred":"arg1Of","subj":"T21635","obj":"T21636"},{"id":"R14808","pred":"arg1Of","subj":"T21636","obj":"T21638"},{"id":"R14809","pred":"arg2Of","subj":"T21637","obj":"T21636"},{"id":"R14810","pred":"arg1Of","subj":"T21638","obj":"T21640"},{"id":"R14811","pred":"arg2Of","subj":"T21639","obj":"T21638"},{"id":"R14812","pred":"arg2Of","subj":"T21641","obj":"T21640"},{"id":"R14813","pred":"arg2Of","subj":"T21643","obj":"T21632"},{"id":"R14814","pred":"arg1Of","subj":"T21643","obj":"T21633"},{"id":"R14815","pred":"arg1Of","subj":"T21643","obj":"T21634"},{"id":"R14816","pred":"arg1Of","subj":"T21643","obj":"T21635"},{"id":"R14817","pred":"arg1Of","subj":"T21643","obj":"T21637"},{"id":"R14818","pred":"arg1Of","subj":"T21643","obj":"T21639"},{"id":"R14819","pred":"arg1Of","subj":"T21643","obj":"T21641"},{"id":"R14820","pred":"arg1Of","subj":"T21643","obj":"T21642"},{"id":"R14821","pred":"arg1Of","subj":"T21643","obj":"T21644"},{"id":"R14822","pred":"arg1Of","subj":"T21644","obj":"T21645"},{"id":"R14823","pred":"arg1Of","subj":"T21647","obj":"T21648"},{"id":"R14824","pred":"arg1Of","subj":"T21648","obj":"T21650"},{"id":"R14825","pred":"arg2Of","subj":"T21649","obj":"T21648"},{"id":"R14826","pred":"arg1Of","subj":"T21650","obj":"T21652"},{"id":"R14827","pred":"arg2Of","subj":"T21651","obj":"T21650"},{"id":"R14828","pred":"arg2Of","subj":"T21653","obj":"T21652"},{"id":"R14829","pred":"arg2Of","subj":"T21655","obj":"T21645"},{"id":"R14830","pred":"arg1Of","subj":"T21655","obj":"T21646"},{"id":"R14831","pred":"arg1Of","subj":"T21655","obj":"T21647"},{"id":"R14832","pred":"arg1Of","subj":"T21655","obj":"T21649"},{"id":"R14833","pred":"arg1Of","subj":"T21655","obj":"T21651"},{"id":"R14834","pred":"arg1Of","subj":"T21655","obj":"T21653"},{"id":"R14835","pred":"arg1Of","subj":"T21655","obj":"T21654"},{"id":"R14836","pred":"arg1Of","subj":"T21655","obj":"T21656"},{"id":"R14837","pred":"arg2Of","subj":"T21658","obj":"T21656"},{"id":"R14838","pred":"arg1Of","subj":"T21658","obj":"T21657"},{"id":"R14839","pred":"arg3Of","subj":"T21659","obj":"T21656"},{"id":"R14840","pred":"arg1Of","subj":"T21660","obj":"T21661"},{"id":"R14841","pred":"arg1Of","subj":"T21660","obj":"T21665"},{"id":"R14842","pred":"arg2Of","subj":"T21664","obj":"T21661"},{"id":"R14843","pred":"arg1Of","subj":"T21664","obj":"T21662"},{"id":"R14844","pred":"arg1Of","subj":"T21664","obj":"T21663"},{"id":"R14845","pred":"arg1Of","subj":"T21665","obj":"T21678"},{"id":"R14846","pred":"arg2Of","subj":"T21667","obj":"T21665"},{"id":"R14847","pred":"arg1Of","subj":"T21667","obj":"T21666"},{"id":"R14848","pred":"arg1Of","subj":"T21667","obj":"T21668"},{"id":"R14849","pred":"arg1Of","subj":"T21670","obj":"T21671"},{"id":"R14850","pred":"arg1Of","subj":"T21671","obj":"T21673"},{"id":"R14851","pred":"arg2Of","subj":"T21672","obj":"T21671"},{"id":"R14852","pred":"arg1Of","subj":"T21673","obj":"T21675"},{"id":"R14853","pred":"arg2Of","subj":"T21674","obj":"T21673"},{"id":"R14854","pred":"arg2Of","subj":"T21676","obj":"T21675"},{"id":"R14855","pred":"arg2Of","subj":"T21677","obj":"T21668"},{"id":"R14856","pred":"arg1Of","subj":"T21677","obj":"T21669"},{"id":"R14857","pred":"arg1Of","subj":"T21677","obj":"T21670"},{"id":"R14858","pred":"arg1Of","subj":"T21677","obj":"T21672"},{"id":"R14859","pred":"arg1Of","subj":"T21677","obj":"T21674"},{"id":"R14860","pred":"arg1Of","subj":"T21677","obj":"T21676"},{"id":"R14861","pred":"arg2Of","subj":"T21679","obj":"T21678"},{"id":"R14862","pred":"arg2Of","subj":"T21681","obj":"T21679"},{"id":"R14863","pred":"arg1Of","subj":"T21681","obj":"T21680"},{"id":"R14864","pred":"arg1Of","subj":"T21681","obj":"T21682"},{"id":"R14865","pred":"arg2Of","subj":"T21684","obj":"T21682"},{"id":"R14866","pred":"arg1Of","subj":"T21684","obj":"T21683"},{"id":"R14867","pred":"arg3Of","subj":"T21685","obj":"T21682"},{"id":"R14868","pred":"arg1Of","subj":"T21686","obj":"T21687"},{"id":"R14869","pred":"arg1Of","subj":"T21686","obj":"T21691"},{"id":"R14870","pred":"arg2Of","subj":"T21690","obj":"T21687"},{"id":"R14871","pred":"arg1Of","subj":"T21690","obj":"T21688"},{"id":"R14872","pred":"arg1Of","subj":"T21690","obj":"T21689"},{"id":"R14873","pred":"arg1Of","subj":"T21691","obj":"T21698"},{"id":"R14874","pred":"arg1Of","subj":"T21691","obj":"T21699"},{"id":"R14875","pred":"arg2Of","subj":"T21693","obj":"T21691"},{"id":"R14876","pred":"arg1Of","subj":"T21693","obj":"T21692"},{"id":"R14877","pred":"arg1Of","subj":"T21693","obj":"T21694"},{"id":"R14878","pred":"arg1Of","subj":"T21695","obj":"T21696"},{"id":"R14879","pred":"arg2Of","subj":"T21696","obj":"T21694"},{"id":"R14880","pred":"arg2Of","subj":"T21697","obj":"T21696"},{"id":"R14881","pred":"arg1Of","subj":"T21701","obj":"T21700"},{"id":"R14882","pred":"arg1Of","subj":"T21701","obj":"T21702"},{"id":"R14883","pred":"arg1Of","subj":"T21701","obj":"T21706"},{"id":"R14884","pred":"arg2Of","subj":"T21701","obj":"T21707"},{"id":"R14885","pred":"arg2Of","subj":"T21705","obj":"T21702"},{"id":"R14886","pred":"arg1Of","subj":"T21705","obj":"T21703"},{"id":"R14887","pred":"arg1Of","subj":"T21705","obj":"T21704"},{"id":"R14888","pred":"arg2Of","subj":"T21707","obj":"T21706"},{"id":"R14889","pred":"arg1Of","subj":"T21707","obj":"T21708"},{"id":"R14890","pred":"arg2Of","subj":"T21709","obj":"T21708"},{"id":"R14891","pred":"arg1Of","subj":"T21709","obj":"T21710"},{"id":"R14892","pred":"arg1Of","subj":"T21709","obj":"T21711"},{"id":"R14893","pred":"arg3Of","subj":"T21712","obj":"T21708"},{"id":"R14894","pred":"arg1Of","subj":"T21713","obj":"T21715"},{"id":"R14895","pred":"arg1Of","subj":"T21715","obj":"T21714"},{"id":"R14896","pred":"arg2Of","subj":"T21717","obj":"T21715"},{"id":"R14897","pred":"arg1Of","subj":"T21717","obj":"T21716"},{"id":"R14898","pred":"arg1Of","subj":"T21717","obj":"T21718"},{"id":"R14899","pred":"arg1Of","subj":"T21717","obj":"T21720"},{"id":"R14900","pred":"arg1Of","subj":"T21717","obj":"T21724"},{"id":"R14901","pred":"arg1Of","subj":"T21717","obj":"T21725"},{"id":"R14902","pred":"arg2Of","subj":"T21719","obj":"T21718"},{"id":"R14903","pred":"arg2Of","subj":"T21723","obj":"T21720"},{"id":"R14904","pred":"arg1Of","subj":"T21723","obj":"T21721"},{"id":"R14905","pred":"arg1Of","subj":"T21723","obj":"T21722"},{"id":"R14906","pred":"arg2Of","subj":"T21726","obj":"T21725"},{"id":"R14907","pred":"arg1Of","subj":"T21727","obj":"T21728"},{"id":"R14908","pred":"arg1Of","subj":"T21728","obj":"T21731"},{"id":"R14909","pred":"arg2Of","subj":"T21729","obj":"T21728"},{"id":"R14910","pred":"arg2Of","subj":"T21731","obj":"T21726"},{"id":"R14911","pred":"arg1Of","subj":"T21731","obj":"T21730"},{"id":"R14912","pred":"arg2Of","subj":"T21732","obj":"T21731"},{"id":"R14913","pred":"arg1Of","subj":"T21734","obj":"T21733"},{"id":"R14914","pred":"arg1Of","subj":"T21734","obj":"T21735"},{"id":"R14915","pred":"arg2Of","subj":"T21734","obj":"T21736"},{"id":"R14916","pred":"arg1Of","subj":"T21734","obj":"T21751"},{"id":"R14917","pred":"arg2Of","subj":"T21736","obj":"T21735"},{"id":"R14918","pred":"arg1Of","subj":"T21736","obj":"T21737"},{"id":"R14919","pred":"modOf","subj":"T21736","obj":"T21751"},{"id":"R14920","pred":"arg1Of","subj":"T21739","obj":"T21738"},{"id":"R14921","pred":"arg1Of","subj":"T21739","obj":"T21740"},{"id":"R14922","pred":"arg1Of","subj":"T21740","obj":"T21742"},{"id":"R14923","pred":"arg2Of","subj":"T21741","obj":"T21740"},{"id":"R14924","pred":"arg1Of","subj":"T21742","obj":"T21744"},{"id":"R14925","pred":"arg2Of","subj":"T21743","obj":"T21742"},{"id":"R14926","pred":"arg2Of","subj":"T21744","obj":"T21737"},{"id":"R14927","pred":"arg2Of","subj":"T21745","obj":"T21744"},{"id":"R14928","pred":"arg1Of","subj":"T21745","obj":"T21746"},{"id":"R14929","pred":"arg2Of","subj":"T21748","obj":"T21746"},{"id":"R14930","pred":"arg1Of","subj":"T21748","obj":"T21747"},{"id":"R14931","pred":"arg3Of","subj":"T21749","obj":"T21746"},{"id":"R14932","pred":"arg1Of","subj":"T21751","obj":"T21750"},{"id":"R14933","pred":"arg1Of","subj":"T21753","obj":"T21754"},{"id":"R14934","pred":"arg1Of","subj":"T21754","obj":"T21758"},{"id":"R14935","pred":"arg1Of","subj":"T21754","obj":"T21769"},{"id":"R14936","pred":"arg2Of","subj":"T21754","obj":"T21770"},{"id":"R14937","pred":"arg2Of","subj":"T21757","obj":"T21754"},{"id":"R14938","pred":"arg1Of","subj":"T21757","obj":"T21755"},{"id":"R14939","pred":"arg1Of","subj":"T21757","obj":"T21756"},{"id":"R14940","pred":"arg2Of","subj":"T21760","obj":"T21758"},{"id":"R14941","pred":"arg1Of","subj":"T21760","obj":"T21759"},{"id":"R14942","pred":"arg1Of","subj":"T21760","obj":"T21761"},{"id":"R14943","pred":"arg1Of","subj":"T21763","obj":"T21762"},{"id":"R14944","pred":"arg2Of","subj":"T21764","obj":"T21761"},{"id":"R14945","pred":"arg1Of","subj":"T21764","obj":"T21763"},{"id":"R14946","pred":"arg1Of","subj":"T21764","obj":"T21765"},{"id":"R14947","pred":"arg2Of","subj":"T21768","obj":"T21765"},{"id":"R14948","pred":"arg1Of","subj":"T21768","obj":"T21766"},{"id":"R14949","pred":"arg1Of","subj":"T21768","obj":"T21767"},{"id":"R14950","pred":"arg2Of","subj":"T21770","obj":"T21751"},{"id":"R14951","pred":"arg1Of","subj":"T21770","obj":"T21752"},{"id":"R14952","pred":"arg2Of","subj":"T21770","obj":"T21769"},{"id":"R14953","pred":"arg1Of","subj":"T21772","obj":"T21771"},{"id":"R14954","pred":"arg1Of","subj":"T21772","obj":"T21773"},{"id":"R14955","pred":"arg1Of","subj":"T21772","obj":"T21777"},{"id":"R14956","pred":"arg1Of","subj":"T21772","obj":"T21794"},{"id":"R14957","pred":"arg2Of","subj":"T21776","obj":"T21773"},{"id":"R14958","pred":"arg1Of","subj":"T21776","obj":"T21774"},{"id":"R14959","pred":"arg1Of","subj":"T21776","obj":"T21775"},{"id":"R14960","pred":"arg1Of","subj":"T21778","obj":"T21779"},{"id":"R14961","pred":"arg1Of","subj":"T21778","obj":"T21785"},{"id":"R14962","pred":"arg1Of","subj":"T21780","obj":"T21781"},{"id":"R14963","pred":"arg2Of","subj":"T21782","obj":"T21781"},{"id":"R14964","pred":"arg2Of","subj":"T21783","obj":"T21779"},{"id":"R14965","pred":"arg1Of","subj":"T21783","obj":"T21780"},{"id":"R14966","pred":"arg1Of","subj":"T21783","obj":"T21782"},{"id":"R14967","pred":"arg3Of","subj":"T21784","obj":"T21779"},{"id":"R14968","pred":"arg2Of","subj":"T21785","obj":"T21777"},{"id":"R14969","pred":"arg2Of","subj":"T21787","obj":"T21785"},{"id":"R14970","pred":"arg1Of","subj":"T21787","obj":"T21786"},{"id":"R14971","pred":"arg1Of","subj":"T21787","obj":"T21788"},{"id":"R14972","pred":"arg2Of","subj":"T21790","obj":"T21788"},{"id":"R14973","pred":"arg1Of","subj":"T21790","obj":"T21789"},{"id":"R14974","pred":"arg3Of","subj":"T21791","obj":"T21788"},{"id":"R14975","pred":"arg1Of","subj":"T21794","obj":"T21792"},{"id":"R14976","pred":"arg1Of","subj":"T21794","obj":"T21793"},{"id":"R14977","pred":"arg1Of","subj":"T21794","obj":"T21800"},{"id":"R14978","pred":"arg2Of","subj":"T21796","obj":"T21794"},{"id":"R14979","pred":"arg1Of","subj":"T21796","obj":"T21795"},{"id":"R14980","pred":"arg1Of","subj":"T21796","obj":"T21797"},{"id":"R14981","pred":"arg2Of","subj":"T21799","obj":"T21797"},{"id":"R14982","pred":"arg1Of","subj":"T21799","obj":"T21798"},{"id":"R14983","pred":"arg2Of","subj":"T21803","obj":"T21800"},{"id":"R14984","pred":"arg1Of","subj":"T21803","obj":"T21801"},{"id":"R14985","pred":"arg1Of","subj":"T21803","obj":"T21802"},{"id":"R14986","pred":"arg1Of","subj":"T21803","obj":"T21804"},{"id":"R14987","pred":"arg2Of","subj":"T21806","obj":"T21804"},{"id":"R14988","pred":"arg1Of","subj":"T21806","obj":"T21805"},{"id":"R14989","pred":"arg3Of","subj":"T21807","obj":"T21804"},{"id":"R14990","pred":"arg1Of","subj":"T21810","obj":"T21808"},{"id":"R14991","pred":"arg1Of","subj":"T21810","obj":"T21809"},{"id":"R14992","pred":"arg1Of","subj":"T21810","obj":"T21811"},{"id":"R14993","pred":"arg2Of","subj":"T21810","obj":"T21812"},{"id":"R14994","pred":"arg2Of","subj":"T21812","obj":"T21811"},{"id":"R14995","pred":"arg3Of","subj":"T21813","obj":"T21812"},{"id":"R14996","pred":"modOf","subj":"T21813","obj":"T21826"},{"id":"R14997","pred":"arg1Of","subj":"T21815","obj":"T21814"},{"id":"R14998","pred":"arg1Of","subj":"T21815","obj":"T21826"},{"id":"R14999","pred":"arg2Of","subj":"T21815","obj":"T21827"},{"id":"R15000","pred":"arg1Of","subj":"T21816","obj":"T21817"},{"id":"R15001","pred":"arg2Of","subj":"T21817","obj":"T21815"},{"id":"R15002","pred":"arg1Of","subj":"T21817","obj":"T21819"},{"id":"R15003","pred":"arg2Of","subj":"T21818","obj":"T21817"},{"id":"R15004","pred":"arg2Of","subj":"T21822","obj":"T21819"},{"id":"R15005","pred":"arg1Of","subj":"T21822","obj":"T21820"},{"id":"R15006","pred":"arg1Of","subj":"T21822","obj":"T21821"},{"id":"R15007","pred":"arg1Of","subj":"T21822","obj":"T21823"},{"id":"R15008","pred":"arg1Of","subj":"T21822","obj":"T21824"},{"id":"R15009","pred":"arg3Of","subj":"T21825","obj":"T21819"},{"id":"R15010","pred":"arg2Of","subj":"T21827","obj":"T21826"},{"id":"R15011","pred":"arg1Of","subj":"T21827","obj":"T21828"},{"id":"R15012","pred":"arg2Of","subj":"T21829","obj":"T21828"},{"id":"R15013","pred":"arg1Of","subj":"T21829","obj":"T21830"},{"id":"R15014","pred":"arg2Of","subj":"T21832","obj":"T21830"},{"id":"R15015","pred":"arg1Of","subj":"T21832","obj":"T21831"},{"id":"R15016","pred":"arg1Of","subj":"T21832","obj":"T21833"},{"id":"R15017","pred":"arg2Of","subj":"T21834","obj":"T21833"},{"id":"R15018","pred":"arg1Of","subj":"T21834","obj":"T21835"},{"id":"R15019","pred":"arg1Of","subj":"T21834","obj":"T21836"},{"id":"R15020","pred":"arg3Of","subj":"T21837","obj":"T21833"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T21515","span":{"begin":998,"end":1004},"obj":"Protein"},{"id":"T21514","span":{"begin":816,"end":822},"obj":"Protein"},{"id":"T21513","span":{"begin":790,"end":796},"obj":"Protein"},{"id":"T21512","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T21511","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T21510","span":{"begin":692,"end":698},"obj":"Protein"},{"id":"T21509","span":{"begin":557,"end":560},"obj":"Protein"},{"id":"T21508","span":{"begin":548,"end":551},"obj":"Protein"},{"id":"T21507","span":{"begin":534,"end":538},"obj":"Protein"},{"id":"T21506","span":{"begin":426,"end":430},"obj":"Protein"},{"id":"T21505","span":{"begin":407,"end":413},"obj":"Protein"},{"id":"T21504","span":{"begin":387,"end":391},"obj":"Protein"},{"id":"T21503","span":{"begin":292,"end":296},"obj":"Protein"},{"id":"T21502","span":{"begin":283,"end":287},"obj":"Protein"},{"id":"T21501","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T21500","span":{"begin":271,"end":275},"obj":"Protein"},{"id":"T21499","span":{"begin":223,"end":227},"obj":"Protein"},{"id":"T21498","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T21497","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T21496","span":{"begin":53,"end":59},"obj":"Protein"},{"id":"T21495","span":{"begin":38,"end":42},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T21844","span":{"begin":548,"end":551},"obj":"http://www.uniprot.org/uniprot/Q04206"},{"id":"T21843","span":{"begin":283,"end":287},"obj":"http://www.uniprot.org/uniprot/P05231"},{"id":"T21842","span":{"begin":277,"end":281},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T21841","span":{"begin":534,"end":538},"obj":"http://www.uniprot.org/uniprot/P10145"},{"id":"T21840","span":{"begin":426,"end":430},"obj":"http://www.uniprot.org/uniprot/P10145"},{"id":"T21839","span":{"begin":292,"end":296},"obj":"http://www.uniprot.org/uniprot/P10145"},{"id":"T21838","span":{"begin":223,"end":227},"obj":"http://www.uniprot.org/uniprot/P10145"},{"id":"T21853","span":{"begin":1111,"end":1117},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21852","span":{"begin":998,"end":1004},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21851","span":{"begin":816,"end":822},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21850","span":{"begin":790,"end":796},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21849","span":{"begin":727,"end":733},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21848","span":{"begin":712,"end":718},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21847","span":{"begin":692,"end":698},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21846","span":{"begin":557,"end":560},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T21845","span":{"begin":548,"end":551},"obj":"http://www.uniprot.org/uniprot/P21579"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"UBERON-AE","denotations":[{"id":"T21460","span":{"begin":498,"end":502},"obj":"http://purl.obolibrary.org/obo/UBERON_0000033"},{"id":"T21459","span":{"begin":345,"end":349},"obj":"http://purl.obolibrary.org/obo/UBERON_0000033"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"GO-MF","denotations":[{"id":"T21527","span":{"begin":283,"end":287},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T21526","span":{"begin":534,"end":538},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T21525","span":{"begin":426,"end":430},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T21524","span":{"begin":292,"end":296},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T21523","span":{"begin":223,"end":227},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T21522","span":{"begin":27,"end":42},"obj":"http://purl.obolibrary.org/obo/GO_0051525"},{"id":"T21521","span":{"begin":950,"end":957},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T21520","span":{"begin":661,"end":668},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T21519","span":{"begin":27,"end":34},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T21518","span":{"begin":946,"end":957},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T21517","span":{"begin":657,"end":668},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T21516","span":{"begin":23,"end":34},"obj":"http://purl.obolibrary.org/obo/GO_0003677"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"sentences","denotations":[{"id":"T21469","span":{"begin":1126,"end":1255},"obj":"Sentence"},{"id":"T21468","span":{"begin":913,"end":1125},"obj":"Sentence"},{"id":"T21467","span":{"begin":738,"end":912},"obj":"Sentence"},{"id":"T21466","span":{"begin":644,"end":737},"obj":"Sentence"},{"id":"T21465","span":{"begin":576,"end":643},"obj":"Sentence"},{"id":"T21464","span":{"begin":478,"end":575},"obj":"Sentence"},{"id":"T21463","span":{"begin":323,"end":477},"obj":"Sentence"},{"id":"T21462","span":{"begin":61,"end":322},"obj":"Sentence"},{"id":"T21461","span":{"begin":10,"end":60},"obj":"Sentence"},{"id":"T68","span":{"begin":0,"end":60},"obj":"Sentence"},{"id":"T69","span":{"begin":61,"end":144},"obj":"Sentence"},{"id":"T70","span":{"begin":145,"end":322},"obj":"Sentence"},{"id":"T71","span":{"begin":323,"end":477},"obj":"Sentence"},{"id":"T72","span":{"begin":478,"end":575},"obj":"Sentence"},{"id":"T73","span":{"begin":576,"end":643},"obj":"Sentence"},{"id":"T74","span":{"begin":644,"end":737},"obj":"Sentence"},{"id":"T75","span":{"begin":738,"end":852},"obj":"Sentence"},{"id":"T76","span":{"begin":853,"end":912},"obj":"Sentence"},{"id":"T77","span":{"begin":913,"end":1125},"obj":"Sentence"},{"id":"T78","span":{"begin":1126,"end":1255},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"simple1","denotations":[{"id":"T21594","span":{"begin":998,"end":1004},"obj":"Protein"},{"id":"T21593","span":{"begin":816,"end":822},"obj":"Protein"},{"id":"T21592","span":{"begin":790,"end":796},"obj":"Protein"},{"id":"T21591","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T21590","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T21589","span":{"begin":692,"end":698},"obj":"Protein"},{"id":"T21588","span":{"begin":557,"end":560},"obj":"Protein"},{"id":"T21587","span":{"begin":548,"end":551},"obj":"Protein"},{"id":"T21586","span":{"begin":534,"end":538},"obj":"Protein"},{"id":"T21585","span":{"begin":426,"end":430},"obj":"Protein"},{"id":"T21584","span":{"begin":407,"end":413},"obj":"Protein"},{"id":"T21583","span":{"begin":387,"end":391},"obj":"Protein"},{"id":"T21582","span":{"begin":292,"end":296},"obj":"Protein"},{"id":"T21581","span":{"begin":283,"end":287},"obj":"Protein"},{"id":"T21580","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T21579","span":{"begin":271,"end":275},"obj":"Protein"},{"id":"T21578","span":{"begin":223,"end":227},"obj":"Protein"},{"id":"T21577","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T21576","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T21575","span":{"begin":53,"end":59},"obj":"Protein"},{"id":"T21574","span":{"begin":38,"end":42},"obj":"Protein"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T22281","span":{"begin":27,"end":34},"obj":"Binding"},{"id":"T22280","span":{"begin":998,"end":1004},"obj":"Protein"},{"id":"T22279","span":{"begin":816,"end":822},"obj":"Protein"},{"id":"T22278","span":{"begin":790,"end":796},"obj":"Protein"},{"id":"T22277","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T22276","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T22275","span":{"begin":692,"end":698},"obj":"Protein"},{"id":"T22274","span":{"begin":557,"end":560},"obj":"Protein"},{"id":"T22273","span":{"begin":548,"end":551},"obj":"Protein"},{"id":"T22272","span":{"begin":534,"end":538},"obj":"Protein"},{"id":"T22271","span":{"begin":426,"end":430},"obj":"Protein"},{"id":"T22270","span":{"begin":407,"end":413},"obj":"Protein"},{"id":"T22269","span":{"begin":387,"end":391},"obj":"Protein"},{"id":"T22268","span":{"begin":292,"end":296},"obj":"Protein"},{"id":"T22267","span":{"begin":283,"end":287},"obj":"Protein"},{"id":"T22266","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T22265","span":{"begin":271,"end":275},"obj":"Protein"},{"id":"T22264","span":{"begin":223,"end":227},"obj":"Protein"},{"id":"T22263","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T22262","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T22261","span":{"begin":53,"end":59},"obj":"Protein"},{"id":"T22260","span":{"begin":38,"end":42},"obj":"Protein"}],"relations":[{"id":"R15305","pred":"themeOf","subj":"T22260","obj":"T22281"},{"id":"R15306","pred":"themeOf","subj":"T22261","obj":"T22281"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T21854","span":{"begin":38,"end":42},"obj":"Protein"},{"id":"T21878","span":{"begin":661,"end":668},"obj":"Binding"},{"id":"T21877","span":{"begin":647,"end":656},"obj":"Negative_regulation"},{"id":"T21876","span":{"begin":27,"end":34},"obj":"Binding"},{"id":"T21875","span":{"begin":16,"end":19},"obj":"Positive_regulation"},{"id":"T21874","span":{"begin":998,"end":1004},"obj":"Protein"},{"id":"T21873","span":{"begin":816,"end":822},"obj":"Protein"},{"id":"T21872","span":{"begin":790,"end":796},"obj":"Protein"},{"id":"T21871","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T21870","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T21869","span":{"begin":692,"end":698},"obj":"Protein"},{"id":"T21868","span":{"begin":557,"end":560},"obj":"Protein"},{"id":"T21867","span":{"begin":548,"end":551},"obj":"Protein"},{"id":"T21866","span":{"begin":534,"end":538},"obj":"Protein"},{"id":"T21865","span":{"begin":426,"end":430},"obj":"Protein"},{"id":"T21864","span":{"begin":407,"end":413},"obj":"Protein"},{"id":"T21863","span":{"begin":387,"end":391},"obj":"Protein"},{"id":"T21862","span":{"begin":292,"end":296},"obj":"Protein"},{"id":"T21861","span":{"begin":283,"end":287},"obj":"Protein"},{"id":"T21860","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T21859","span":{"begin":271,"end":275},"obj":"Protein"},{"id":"T21858","span":{"begin":223,"end":227},"obj":"Protein"},{"id":"T21857","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T21856","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T21855","span":{"begin":53,"end":59},"obj":"Protein"}],"relations":[{"id":"R15021","pred":"themeOf","subj":"T21854","obj":"T21876"},{"id":"R15022","pred":"themeOf","subj":"T21855","obj":"T21876"},{"id":"R15023","pred":"themeOf","subj":"T21869","obj":"T21878"},{"id":"R15024","pred":"themeOf","subj":"T21876","obj":"T21875"},{"id":"R15025","pred":"themeOf","subj":"T21876","obj":"T21875"},{"id":"R15026","pred":"themeOf","subj":"T21878","obj":"T21877"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"DLUT931","denotations":[{"id":"T21904","span":{"begin":647,"end":656},"obj":"Negative_regulation"},{"id":"T21903","span":{"begin":661,"end":668},"obj":"Binding"},{"id":"T21902","span":{"begin":250,"end":263},"obj":"Positive_regulation"},{"id":"T21901","span":{"begin":16,"end":19},"obj":"Positive_regulation"},{"id":"T21900","span":{"begin":27,"end":34},"obj":"Binding"},{"id":"T21899","span":{"begin":998,"end":1004},"obj":"Protein"},{"id":"T21898","span":{"begin":816,"end":822},"obj":"Protein"},{"id":"T21897","span":{"begin":790,"end":796},"obj":"Protein"},{"id":"T21896","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T21895","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T21894","span":{"begin":692,"end":698},"obj":"Protein"},{"id":"T21893","span":{"begin":557,"end":560},"obj":"Protein"},{"id":"T21892","span":{"begin":548,"end":551},"obj":"Protein"},{"id":"T21891","span":{"begin":534,"end":538},"obj":"Protein"},{"id":"T21890","span":{"begin":426,"end":430},"obj":"Protein"},{"id":"T21889","span":{"begin":407,"end":413},"obj":"Protein"},{"id":"T21888","span":{"begin":387,"end":391},"obj":"Protein"},{"id":"T21887","span":{"begin":292,"end":296},"obj":"Protein"},{"id":"T21886","span":{"begin":283,"end":287},"obj":"Protein"},{"id":"T21885","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T21884","span":{"begin":271,"end":275},"obj":"Protein"},{"id":"T21883","span":{"begin":223,"end":227},"obj":"Protein"},{"id":"T21882","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T21881","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T21880","span":{"begin":53,"end":59},"obj":"Protein"},{"id":"T21879","span":{"begin":38,"end":42},"obj":"Protein"}],"relations":[{"id":"R15027","pred":"themeOf","subj":"T21879","obj":"T21900"},{"id":"R15028","pred":"themeOf","subj":"T21879","obj":"T21900"},{"id":"R15029","pred":"themeOf","subj":"T21880","obj":"T21900"},{"id":"R15030","pred":"themeOf","subj":"T21884","obj":"T21902"},{"id":"R15031","pred":"themeOf","subj":"T21885","obj":"T21902"},{"id":"R15032","pred":"themeOf","subj":"T21886","obj":"T21902"},{"id":"R15033","pred":"themeOf","subj":"T21894","obj":"T21903"},{"id":"R15034","pred":"themeOf","subj":"T21900","obj":"T21901"},{"id":"R15035","pred":"themeOf","subj":"T21900","obj":"T21901"},{"id":"R15036","pred":"themeOf","subj":"T21903","obj":"T21904"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T22259","span":{"begin":798,"end":809},"obj":"Protein"},{"id":"T22258","span":{"begin":431,"end":434},"obj":"Entity"},{"id":"T22257","span":{"begin":218,"end":227},"obj":"Protein"},{"id":"T22256","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T22255","span":{"begin":44,"end":48},"obj":"Protein"},{"id":"T22254","span":{"begin":271,"end":282},"obj":"Protein"},{"id":"T22253","span":{"begin":264,"end":311},"obj":"Protein"},{"id":"T22252","span":{"begin":785,"end":788},"obj":"Entity"},{"id":"T22251","span":{"begin":67,"end":71},"obj":"Entity"},{"id":"T22250","span":{"begin":790,"end":813},"obj":"Protein"},{"id":"T22249","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T22248","span":{"begin":977,"end":994},"obj":"Protein"},{"id":"T22247","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T22246","span":{"begin":145,"end":152},"obj":"Entity"},{"id":"T22245","span":{"begin":264,"end":311},"obj":"Entity"},{"id":"T22244","span":{"begin":1111,"end":1117},"obj":"Protein"},{"id":"T22243","span":{"begin":738,"end":748},"obj":"Entity"},{"id":"T22242","span":{"begin":557,"end":561},"obj":"Protein"},{"id":"T22241","span":{"begin":407,"end":417},"obj":"Entity"},{"id":"T22240","span":{"begin":397,"end":405},"obj":"Entity"},{"id":"T22239","span":{"begin":53,"end":59},"obj":"Protein"},{"id":"T22238","span":{"begin":421,"end":425},"obj":"Protein"},{"id":"T22237","span":{"begin":687,"end":689},"obj":"Protein"},{"id":"T22236","span":{"begin":832,"end":851},"obj":"Entity"},{"id":"T22235","span":{"begin":941,"end":957},"obj":"Protein"},{"id":"T22234","span":{"begin":218,"end":243},"obj":"Entity"},{"id":"T22233","span":{"begin":672,"end":676},"obj":"Protein"},{"id":"T22232","span":{"begin":38,"end":42},"obj":"Protein"},{"id":"T22231","span":{"begin":383,"end":395},"obj":"Entity"},{"id":"T22230","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T22229","span":{"begin":132,"end":135},"obj":"Entity"},{"id":"T22228","span":{"begin":1108,"end":1122},"obj":"Protein"},{"id":"T22227","span":{"begin":218,"end":227},"obj":"Protein"},{"id":"T22226","span":{"begin":816,"end":822},"obj":"Protein"},{"id":"T22225","span":{"begin":998,"end":1004},"obj":"Protein"},{"id":"T22224","span":{"begin":426,"end":430},"obj":"Protein"},{"id":"T22223","span":{"begin":223,"end":227},"obj":"Protein"},{"id":"T22222","span":{"begin":692,"end":699},"obj":"Protein"},{"id":"T22221","span":{"begin":397,"end":405},"obj":"Protein"},{"id":"T22220","span":{"begin":153,"end":161},"obj":"Entity"},{"id":"T22219","span":{"begin":292,"end":296},"obj":"Protein"},{"id":"T22218","span":{"begin":421,"end":442},"obj":"Entity"},{"id":"T22217","span":{"begin":790,"end":810},"obj":"Protein"},{"id":"T22216","span":{"begin":202,"end":206},"obj":"Protein"},{"id":"T22215","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T22214","span":{"begin":283,"end":287},"obj":"Protein"}],"relations":[{"id":"R15304","pred":"partOf","subj":"T22245","obj":"T22254"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T22185","span":{"begin":1250,"end":1251},"obj":"SYM"},{"id":"T22184","span":{"begin":1248,"end":1249},"obj":"FW"},{"id":"T22183","span":{"begin":1247,"end":1248},"obj":"-LRB-"},{"id":"T22182","span":{"begin":1239,"end":1246},"obj":"NN"},{"id":"T22181","span":{"begin":1232,"end":1238},"obj":"JJ"},{"id":"T22180","span":{"begin":1229,"end":1231},"obj":"TO"},{"id":"T22179","span":{"begin":1221,"end":1228},"obj":"NN"},{"id":"T22178","span":{"begin":1216,"end":1220},"obj":"IN"},{"id":"T22177","span":{"begin":1210,"end":1215},"obj":"VBN"},{"id":"T22176","span":{"begin":1206,"end":1209},"obj":"VBP"},{"id":"T22175","span":{"begin":1204,"end":1205},"obj":"-RRB-"},{"id":"T22174","span":{"begin":1200,"end":1204},"obj":"CD"},{"id":"T22173","span":{"begin":1198,"end":1199},"obj":"FW"},{"id":"T22172","span":{"begin":1196,"end":1197},"obj":"FW"},{"id":"T22171","span":{"begin":1194,"end":1195},"obj":"SYM"},{"id":"T22170","span":{"begin":1192,"end":1193},"obj":"SYM"},{"id":"T22169","span":{"begin":1191,"end":1192},"obj":"-LRB-"},{"id":"T22168","span":{"begin":1177,"end":1190},"obj":"NNS"},{"id":"T22167","span":{"begin":1173,"end":1176},"obj":"CC"},{"id":"T22166","span":{"begin":1169,"end":1172},"obj":"NNP"},{"id":"T22165","span":{"begin":1167,"end":1168},"obj":"CD"},{"id":"T22164","span":{"begin":1166,"end":1167},"obj":"CD"},{"id":"T22163","span":{"begin":1163,"end":1165},"obj":"TO"},{"id":"T22162","span":{"begin":1157,"end":1162},"obj":"JJ"},{"id":"T22161","span":{"begin":1153,"end":1156},"obj":"VBN"},{"id":"T22160","span":{"begin":1148,"end":1152},"obj":"VBD"},{"id":"T22159","span":{"begin":1141,"end":1147},"obj":"NNS"},{"id":"T22158","span":{"begin":1133,"end":1140},"obj":"NN"},{"id":"T22157","span":{"begin":1126,"end":1132},"obj":"NN"},{"id":"T22156","span":{"begin":1124,"end":1125},"obj":"."},{"id":"T22155","span":{"begin":1123,"end":1124},"obj":"-RRB-"},{"id":"T22154","span":{"begin":1122,"end":1123},"obj":"."},{"id":"T22153","span":{"begin":1118,"end":1122},"obj":"NN"},{"id":"T22152","span":{"begin":1115,"end":1117},"obj":"CD"},{"id":"T22151","span":{"begin":1108,"end":1115},"obj":"NNP"},{"id":"T22150","span":{"begin":1107,"end":1108},"obj":"-LRB-"},{"id":"T22149","span":{"begin":1101,"end":1106},"obj":"NN"},{"id":"T22148","span":{"begin":1089,"end":1100},"obj":"JJ"},{"id":"T22147","span":{"begin":1085,"end":1088},"obj":"DT"},{"id":"T22146","span":{"begin":1081,"end":1084},"obj":"CC"},{"id":"T22145","span":{"begin":1071,"end":1080},"obj":"NNS"},{"id":"T22144","span":{"begin":1066,"end":1070},"obj":"DT"},{"id":"T22143","span":{"begin":1063,"end":1065},"obj":"IN"},{"id":"T22142","span":{"begin":1048,"end":1062},"obj":"NN"},{"id":"T22141","span":{"begin":1035,"end":1047},"obj":"JJ"},{"id":"T22140","span":{"begin":1032,"end":1034},"obj":"IN"},{"id":"T22139","span":{"begin":1027,"end":1031},"obj":"RB"},{"id":"T22138","span":{"begin":1024,"end":1026},"obj":"RB"},{"id":"T22137","span":{"begin":1022,"end":1023},"obj":"-RRB-"},{"id":"T22136","span":{"begin":1020,"end":1022},"obj":"NN"},{"id":"T22135","span":{"begin":1016,"end":1019},"obj":"CD"},{"id":"T22134","span":{"begin":1015,"end":1016},"obj":"-LRB-"},{"id":"T22133","span":{"begin":1005,"end":1014},"obj":"NN"},{"id":"T22132","span":{"begin":998,"end":1004},"obj":"CD"},{"id":"T22131","span":{"begin":995,"end":997},"obj":"CC"},{"id":"T22130","span":{"begin":993,"end":994},"obj":"-RRB-"},{"id":"T22129","span":{"begin":991,"end":993},"obj":"NN"},{"id":"T22128","span":{"begin":987,"end":990},"obj":"CD"},{"id":"T22127","span":{"begin":983,"end":986},"obj":"CC"},{"id":"T22126","span":{"begin":981,"end":982},"obj":"CD"},{"id":"T22125","span":{"begin":980,"end":981},"obj":"-LRB-"},{"id":"T22124","span":{"begin":977,"end":979},"obj":"NNP"},{"id":"T22123","span":{"begin":967,"end":976},"obj":"VBG"},{"id":"T22122","span":{"begin":958,"end":966},"obj":"NN"},{"id":"T22121","span":{"begin":950,"end":957},"obj":"JJ"},{"id":"T22120","span":{"begin":941,"end":949},"obj":"NNP"},{"id":"T22119","span":{"begin":938,"end":940},"obj":"IN"},{"id":"T22118","span":{"begin":923,"end":937},"obj":"NN"},{"id":"T22117","span":{"begin":913,"end":922},"obj":"JJ"},{"id":"T22116","span":{"begin":911,"end":912},"obj":"."},{"id":"T22115","span":{"begin":906,"end":911},"obj":"VBN"},{"id":"T22114","span":{"begin":903,"end":905},"obj":"VBZ"},{"id":"T22113","span":{"begin":892,"end":902},"obj":"NN"},{"id":"T22112","span":{"begin":877,"end":891},"obj":"NN"},{"id":"T22111","span":{"begin":875,"end":876},"obj":"DT"},{"id":"T22110","span":{"begin":870,"end":874},"obj":"IN"},{"id":"T22109","span":{"begin":856,"end":869},"obj":"NN"},{"id":"T22108","span":{"begin":853,"end":855},"obj":"DT"},{"id":"T22107","span":{"begin":851,"end":852},"obj":"."},{"id":"T22106","span":{"begin":850,"end":851},"obj":"NNP"},{"id":"T22105","span":{"begin":849,"end":850},"obj":"CD"},{"id":"T22104","span":{"begin":847,"end":849},"obj":"CD"},{"id":"T22103","span":{"begin":844,"end":846},"obj":"IN"},{"id":"T22102","span":{"begin":842,"end":843},"obj":"NN"},{"id":"T22101","span":{"begin":840,"end":841},"obj":"CD"},{"id":"T22100","span":{"begin":836,"end":839},"obj":"IN"},{"id":"T22099","span":{"begin":832,"end":835},"obj":"NNP"},{"id":"T22098","span":{"begin":830,"end":831},"obj":"NNP"},{"id":"T22097","span":{"begin":827,"end":829},"obj":"NNP"},{"id":"T22096","span":{"begin":823,"end":826},"obj":"CC"},{"id":"T22095","span":{"begin":816,"end":822},"obj":"NNP"},{"id":"T22094","span":{"begin":814,"end":815},"obj":"NNP"},{"id":"T22093","span":{"begin":811,"end":813},"obj":"NNP"},{"id":"T22092","span":{"begin":809,"end":810},"obj":","},{"id":"T22091","span":{"begin":808,"end":809},"obj":"-RRB-"},{"id":"T22090","span":{"begin":806,"end":808},"obj":"NN"},{"id":"T22089","span":{"begin":802,"end":805},"obj":"CD"},{"id":"T22088","span":{"begin":801,"end":802},"obj":"-LRB-"},{"id":"T22087","span":{"begin":798,"end":800},"obj":"NNP"},{"id":"T22086","span":{"begin":796,"end":797},"obj":","},{"id":"T22085","span":{"begin":790,"end":796},"obj":"NNP"},{"id":"T22084","span":{"begin":788,"end":789},"obj":","},{"id":"T22083","span":{"begin":785,"end":788},"obj":"NNP"},{"id":"T22082","span":{"begin":783,"end":784},"obj":","},{"id":"T22081","span":{"begin":776,"end":783},"obj":"NN"},{"id":"T22080","span":{"begin":769,"end":775},"obj":"NN"},{"id":"T22079","span":{"begin":764,"end":768},"obj":"IN"},{"id":"T22078","span":{"begin":754,"end":763},"obj":"VBN"},{"id":"T22077","span":{"begin":749,"end":753},"obj":"VBD"},{"id":"T22076","span":{"begin":744,"end":748},"obj":"NNP"},{"id":"T22075","span":{"begin":738,"end":743},"obj":"NNP"},{"id":"T22074","span":{"begin":736,"end":737},"obj":"."},{"id":"T22073","span":{"begin":734,"end":736},"obj":"NNP"},{"id":"T22072","span":{"begin":733,"end":734},"obj":"CD"},{"id":"T22071","span":{"begin":727,"end":733},"obj":"CD"},{"id":"T22070","span":{"begin":724,"end":726},"obj":"CC"},{"id":"T22069","span":{"begin":722,"end":723},"obj":","},{"id":"T22068","span":{"begin":720,"end":722},"obj":"NNP"},{"id":"T22067","span":{"begin":718,"end":719},"obj":","},{"id":"T22066","span":{"begin":712,"end":718},"obj":"CD"},{"id":"T22065","span":{"begin":709,"end":711},"obj":"TO"},{"id":"T22064","span":{"begin":700,"end":708},"obj":"VBN"},{"id":"T22063","span":{"begin":698,"end":699},"obj":","},{"id":"T22062","span":{"begin":692,"end":698},"obj":"NNP"},{"id":"T22061","span":{"begin":690,"end":691},"obj":"NNP"},{"id":"T22060","span":{"begin":687,"end":689},"obj":"NNP"},{"id":"T22059","span":{"begin":677,"end":686},"obj":"VBG"},{"id":"T22058","span":{"begin":672,"end":676},"obj":"NNP"},{"id":"T22057","span":{"begin":669,"end":671},"obj":"IN"},{"id":"T22056","span":{"begin":661,"end":668},"obj":"JJ"},{"id":"T22055","span":{"begin":657,"end":660},"obj":"NNP"},{"id":"T22054","span":{"begin":647,"end":656},"obj":"VBD"},{"id":"T22053","span":{"begin":645,"end":646},"obj":","},{"id":"T22052","span":{"begin":644,"end":645},"obj":"NNP"},{"id":"T22051","span":{"begin":642,"end":643},"obj":"."},{"id":"T22050","span":{"begin":641,"end":642},"obj":"-RRB-"},{"id":"T22049","span":{"begin":640,"end":641},"obj":"CD"},{"id":"T22048","span":{"begin":638,"end":639},"obj":"SYM"},{"id":"T22047","span":{"begin":636,"end":637},"obj":"FW"},{"id":"T22046","span":{"begin":635,"end":636},"obj":"-LRB-"},{"id":"T22045","span":{"begin":629,"end":634},"obj":"VBN"},{"id":"T22044","span":{"begin":626,"end":628},"obj":"VBZ"},{"id":"T22043","span":{"begin":615,"end":625},"obj":"NN"},{"id":"T22042","span":{"begin":600,"end":614},"obj":"NN"},{"id":"T22041","span":{"begin":598,"end":599},"obj":"DT"},{"id":"T22040","span":{"begin":593,"end":597},"obj":"IN"},{"id":"T22039","span":{"begin":579,"end":592},"obj":"NN"},{"id":"T22038","span":{"begin":576,"end":578},"obj":"DT"},{"id":"T22037","span":{"begin":574,"end":575},"obj":"."},{"id":"T22036","span":{"begin":562,"end":574},"obj":"RB"},{"id":"T22035","span":{"begin":560,"end":561},"obj":","},{"id":"T22034","span":{"begin":557,"end":560},"obj":"NN"},{"id":"T22033","span":{"begin":556,"end":557},"obj":":"},{"id":"T22032","span":{"begin":552,"end":555},"obj":"CC"},{"id":"T22031","span":{"begin":529,"end":551},"obj":"NN"},{"id":"T22030","span":{"begin":526,"end":528},"obj":"IN"},{"id":"T22029","span":{"begin":515,"end":525},"obj":"NN"},{"id":"T22028","span":{"begin":513,"end":514},"obj":"DT"},{"id":"T22027","span":{"begin":503,"end":512},"obj":"VBZ"},{"id":"T22026","span":{"begin":498,"end":502},"obj":"NN"},{"id":"T22025","span":{"begin":493,"end":497},"obj":"JJ"},{"id":"T22024","span":{"begin":489,"end":492},"obj":"DT"},{"id":"T22023","span":{"begin":484,"end":488},"obj":"IN"},{"id":"T22022","span":{"begin":478,"end":483},"obj":"NNP"},{"id":"T22021","span":{"begin":476,"end":477},"obj":"."},{"id":"T22020","span":{"begin":475,"end":476},"obj":"-RRB-"},{"id":"T22019","span":{"begin":470,"end":475},"obj":"NN"},{"id":"T22018","span":{"begin":467,"end":469},"obj":"DT"},{"id":"T22017","span":{"begin":466,"end":467},"obj":"-LRB-"},{"id":"T22016","span":{"begin":460,"end":465},"obj":"NN"},{"id":"T22015","span":{"begin":455,"end":459},"obj":"JJ"},{"id":"T22014","span":{"begin":452,"end":454},"obj":"TO"},{"id":"T22013","span":{"begin":443,"end":451},"obj":"VBN"},{"id":"T22012","span":{"begin":435,"end":442},"obj":"JJ"},{"id":"T22011","span":{"begin":421,"end":434},"obj":"NNP"},{"id":"T22010","span":{"begin":418,"end":420},"obj":"CC"},{"id":"T22009","span":{"begin":407,"end":417},"obj":"NNP"},{"id":"T22008","span":{"begin":405,"end":406},"obj":","},{"id":"T22007","span":{"begin":397,"end":405},"obj":"NNP"},{"id":"T22006","span":{"begin":395,"end":396},"obj":","},{"id":"T22005","span":{"begin":387,"end":395},"obj":"NNP"},{"id":"T22004","span":{"begin":383,"end":386},"obj":"DT"},{"id":"T22003","span":{"begin":380,"end":382},"obj":"IN"},{"id":"T22002","span":{"begin":371,"end":379},"obj":"NN"},{"id":"T22001","span":{"begin":359,"end":370},"obj":"JJ"},{"id":"T22000","span":{"begin":350,"end":358},"obj":"VBP"},{"id":"T21999","span":{"begin":345,"end":349},"obj":"NN"},{"id":"T21998","span":{"begin":339,"end":344},"obj":"JJ"},{"id":"T21997","span":{"begin":335,"end":338},"obj":"DT"},{"id":"T21996","span":{"begin":330,"end":334},"obj":"IN"},{"id":"T21995","span":{"begin":323,"end":329},"obj":"NNS"},{"id":"T21994","span":{"begin":321,"end":322},"obj":"."},{"id":"T21993","span":{"begin":320,"end":321},"obj":"-RRB-"},{"id":"T21992","span":{"begin":319,"end":320},"obj":"CD"},{"id":"T21991","span":{"begin":313,"end":318},"obj":"NNP"},{"id":"T21990","span":{"begin":312,"end":313},"obj":"-LRB-"},{"id":"T21989","span":{"begin":302,"end":311},"obj":"NNS"},{"id":"T21988","span":{"begin":297,"end":301},"obj":"NN"},{"id":"T21987","span":{"begin":292,"end":296},"obj":"NNP"},{"id":"T21986","span":{"begin":288,"end":291},"obj":"CC"},{"id":"T21985","span":{"begin":283,"end":287},"obj":"NNP"},{"id":"T21984","span":{"begin":281,"end":282},"obj":","},{"id":"T21983","span":{"begin":277,"end":281},"obj":"NNP"},{"id":"T21982","span":{"begin":275,"end":276},"obj":","},{"id":"T21981","span":{"begin":271,"end":275},"obj":"NNP"},{"id":"T21980","span":{"begin":267,"end":270},"obj":"DT"},{"id":"T21979","span":{"begin":264,"end":266},"obj":"TO"},{"id":"T21978","span":{"begin":250,"end":263},"obj":"JJ"},{"id":"T21977","span":{"begin":244,"end":249},"obj":"NN"},{"id":"T21976","span":{"begin":228,"end":243},"obj":"NN"},{"id":"T21975","span":{"begin":218,"end":227},"obj":"JJ"},{"id":"T21974","span":{"begin":215,"end":217},"obj":"CC"},{"id":"T21973","span":{"begin":208,"end":214},"obj":"NNP"},{"id":"T21972","span":{"begin":206,"end":207},"obj":","},{"id":"T21971","span":{"begin":202,"end":206},"obj":"NNP"},{"id":"T21970","span":{"begin":200,"end":201},"obj":","},{"id":"T22188","span":{"begin":1254,"end":1255},"obj":"."},{"id":"T22187","span":{"begin":1253,"end":1254},"obj":"-RRB-"},{"id":"T22186","span":{"begin":1252,"end":1253},"obj":"LS"},{"id":"T21969","span":{"begin":196,"end":200},"obj":"NNP"},{"id":"T21968","span":{"begin":184,"end":195},"obj":"JJ"},{"id":"T21967","span":{"begin":182,"end":183},"obj":"DT"},{"id":"T21966","span":{"begin":177,"end":181},"obj":"IN"},{"id":"T21965","span":{"begin":167,"end":176},"obj":"VBN"},{"id":"T21964","span":{"begin":162,"end":166},"obj":"VBD"},{"id":"T21963","span":{"begin":153,"end":161},"obj":"NNS"},{"id":"T21962","span":{"begin":145,"end":152},"obj":"NNP"},{"id":"T21961","span":{"begin":142,"end":144},"obj":"NNP"},{"id":"T21960","span":{"begin":141,"end":142},"obj":"CD"},{"id":"T21959","span":{"begin":139,"end":141},"obj":"CD"},{"id":"T21958","span":{"begin":136,"end":138},"obj":"IN"},{"id":"T21957","span":{"begin":134,"end":135},"obj":"NN"},{"id":"T21956","span":{"begin":132,"end":133},"obj":"CD"},{"id":"T21955","span":{"begin":128,"end":131},"obj":"IN"},{"id":"T21954","span":{"begin":126,"end":127},"obj":"-RRB-"},{"id":"T21953","span":{"begin":124,"end":126},"obj":"NN"},{"id":"T21952","span":{"begin":120,"end":123},"obj":"CD"},{"id":"T21951","span":{"begin":116,"end":119},"obj":"CC"},{"id":"T21950","span":{"begin":114,"end":115},"obj":"CD"},{"id":"T21949","span":{"begin":113,"end":114},"obj":"-LRB-"},{"id":"T21948","span":{"begin":110,"end":112},"obj":"NNP"},{"id":"T21947","span":{"begin":107,"end":109},"obj":"CC"},{"id":"T21946","span":{"begin":99,"end":106},"obj":"NN"},{"id":"T21945","span":{"begin":92,"end":98},"obj":"NN"},{"id":"T21944","span":{"begin":87,"end":91},"obj":"IN"},{"id":"T21943","span":{"begin":77,"end":86},"obj":"VBN"},{"id":"T21942","span":{"begin":72,"end":76},"obj":"VBD"},{"id":"T21941","span":{"begin":67,"end":71},"obj":"NNP"},{"id":"T21940","span":{"begin":61,"end":66},"obj":"NNP"},{"id":"T21939","span":{"begin":59,"end":60},"obj":"."},{"id":"T21938","span":{"begin":53,"end":59},"obj":"NNP"},{"id":"T21937","span":{"begin":49,"end":52},"obj":"CC"},{"id":"T21936","span":{"begin":44,"end":48},"obj":"NNP"},{"id":"T21935","span":{"begin":42,"end":43},"obj":","},{"id":"T21934","span":{"begin":38,"end":42},"obj":"NNP"},{"id":"T21933","span":{"begin":35,"end":37},"obj":"IN"},{"id":"T21932","span":{"begin":27,"end":34},"obj":"JJ"},{"id":"T21931","span":{"begin":23,"end":26},"obj":"NNP"},{"id":"T21930","span":{"begin":20,"end":22},"obj":"TO"},{"id":"T21929","span":{"begin":16,"end":19},"obj":"VBD"},{"id":"T21928","span":{"begin":13,"end":15},"obj":"NNP"},{"id":"T21927","span":{"begin":11,"end":12},"obj":","},{"id":"T21926","span":{"begin":10,"end":11},"obj":"DT"}],"relations":[{"id":"R15037","pred":"advmod","subj":"T21926","obj":"T21929"},{"id":"R15038","pred":"punct","subj":"T21927","obj":"T21929"},{"id":"R15039","pred":"nsubj","subj":"T21928","obj":"T21929"},{"id":"R15040","pred":"ROOT","subj":"T21929","obj":"T21929"},{"id":"R15041","pred":"prep","subj":"T21930","obj":"T21929"},{"id":"R15042","pred":"pobj","subj":"T21931","obj":"T21930"},{"id":"R15043","pred":"advcl","subj":"T21932","obj":"T21929"},{"id":"R15044","pred":"prep","subj":"T21933","obj":"T21932"},{"id":"R15045","pred":"pobj","subj":"T21934","obj":"T21933"},{"id":"R15046","pred":"punct","subj":"T21935","obj":"T21934"},{"id":"R15047","pred":"conj","subj":"T21936","obj":"T21934"},{"id":"R15048","pred":"cc","subj":"T21937","obj":"T21936"},{"id":"R15049","pred":"conj","subj":"T21938","obj":"T21936"},{"id":"R15050","pred":"punct","subj":"T21939","obj":"T21929"},{"id":"R15051","pred":"compound","subj":"T21940","obj":"T21941"},{"id":"R15052","pred":"nsubjpass","subj":"T21941","obj":"T21943"},{"id":"R15053","pred":"auxpass","subj":"T21942","obj":"T21943"},{"id":"R15054","pred":"ROOT","subj":"T21943","obj":"T21943"},{"id":"R15055","pred":"prep","subj":"T21944","obj":"T21943"},{"id":"R15056","pred":"compound","subj":"T21945","obj":"T21946"},{"id":"R15057","pred":"pobj","subj":"T21946","obj":"T21944"},{"id":"R15058","pred":"cc","subj":"T21947","obj":"T21946"},{"id":"R15059","pred":"conj","subj":"T21948","obj":"T21946"},{"id":"R15060","pred":"punct","subj":"T21949","obj":"T21950"},{"id":"R15061","pred":"nummod","subj":"T21950","obj":"T21948"},{"id":"R15062","pred":"cc","subj":"T21951","obj":"T21948"},{"id":"R15063","pred":"nummod","subj":"T21952","obj":"T21953"},{"id":"R15064","pred":"conj","subj":"T21953","obj":"T21948"},{"id":"R15065","pred":"punct","subj":"T21954","obj":"T21953"},{"id":"R15066","pred":"prep","subj":"T21955","obj":"T21943"},{"id":"R15067","pred":"nummod","subj":"T21956","obj":"T21957"},{"id":"R15068","pred":"pobj","subj":"T21957","obj":"T21955"},{"id":"R15069","pred":"prep","subj":"T21958","obj":"T21957"},{"id":"R15070","pred":"nummod","subj":"T21959","obj":"T21960"},{"id":"R15071","pred":"compound","subj":"T21960","obj":"T21962"},{"id":"R15072","pred":"compound","subj":"T21961","obj":"T21962"},{"id":"R15073","pred":"compound","subj":"T21962","obj":"T21963"},{"id":"R15074","pred":"pobj","subj":"T21963","obj":"T21958"},{"id":"R15075","pred":"auxpass","subj":"T21964","obj":"T21965"},{"id":"R15076","pred":"conj","subj":"T21965","obj":"T21943"},{"id":"R15077","pred":"prep","subj":"T21966","obj":"T21965"},{"id":"R15078","pred":"det","subj":"T21967","obj":"T21969"},{"id":"R15079","pred":"compound","subj":"T21968","obj":"T21969"},{"id":"R15080","pred":"pobj","subj":"T21969","obj":"T21966"},{"id":"R15081","pred":"punct","subj":"T21970","obj":"T21969"},{"id":"R15082","pred":"conj","subj":"T21971","obj":"T21969"},{"id":"R15083","pred":"punct","subj":"T21972","obj":"T21971"},{"id":"R15084","pred":"conj","subj":"T21973","obj":"T21971"},{"id":"R15085","pred":"cc","subj":"T21974","obj":"T21973"},{"id":"R15086","pred":"amod","subj":"T21975","obj":"T21977"},{"id":"R15087","pred":"compound","subj":"T21976","obj":"T21977"},{"id":"R15088","pred":"conj","subj":"T21977","obj":"T21973"},{"id":"R15089","pred":"amod","subj":"T21978","obj":"T21977"},{"id":"R15090","pred":"prep","subj":"T21979","obj":"T21978"},{"id":"R15091","pred":"det","subj":"T21980","obj":"T21981"},{"id":"R15092","pred":"nmod","subj":"T21981","obj":"T21989"},{"id":"R15093","pred":"punct","subj":"T21982","obj":"T21981"},{"id":"R15094","pred":"conj","subj":"T21983","obj":"T21981"},{"id":"R15095","pred":"punct","subj":"T21984","obj":"T21983"},{"id":"R15096","pred":"conj","subj":"T21985","obj":"T21983"},{"id":"R15097","pred":"cc","subj":"T21986","obj":"T21985"},{"id":"R15098","pred":"conj","subj":"T21987","obj":"T21985"},{"id":"R15099","pred":"compound","subj":"T21988","obj":"T21989"},{"id":"R15100","pred":"pobj","subj":"T21989","obj":"T21979"},{"id":"R15101","pred":"punct","subj":"T21990","obj":"T21989"},{"id":"R15102","pred":"nmod","subj":"T21991","obj":"T21993"},{"id":"R15103","pred":"nummod","subj":"T21992","obj":"T21991"},{"id":"R15104","pred":"punct","subj":"T21993","obj":"T21989"},{"id":"R15105","pred":"punct","subj":"T21994","obj":"T21943"},{"id":"R15106","pred":"nsubj","subj":"T21995","obj":"T22000"},{"id":"R15107","pred":"prep","subj":"T21996","obj":"T21995"},{"id":"R15108","pred":"det","subj":"T21997","obj":"T21999"},{"id":"R15109","pred":"amod","subj":"T21998","obj":"T21999"},{"id":"R15110","pred":"pobj","subj":"T21999","obj":"T21996"},{"id":"R15111","pred":"ROOT","subj":"T22000","obj":"T22000"},{"id":"R15112","pred":"amod","subj":"T22001","obj":"T22002"},{"id":"R15113","pred":"dobj","subj":"T22002","obj":"T22000"},{"id":"R15114","pred":"prep","subj":"T22003","obj":"T22002"},{"id":"R15115","pred":"det","subj":"T22004","obj":"T22005"},{"id":"R15116","pred":"pobj","subj":"T22005","obj":"T22003"},{"id":"R15117","pred":"punct","subj":"T22006","obj":"T22005"},{"id":"R15118","pred":"conj","subj":"T22007","obj":"T22005"},{"id":"R15119","pred":"punct","subj":"T22008","obj":"T22007"},{"id":"R15120","pred":"conj","subj":"T22009","obj":"T22007"},{"id":"R15121","pred":"cc","subj":"T22010","obj":"T22009"},{"id":"R15122","pred":"conj","subj":"T22011","obj":"T22009"},{"id":"R15123","pred":"amod","subj":"T22012","obj":"T22013"},{"id":"R15124","pred":"prep","subj":"T22013","obj":"T22000"},{"id":"R15125","pred":"prep","subj":"T22014","obj":"T22013"},{"id":"R15126","pred":"amod","subj":"T22015","obj":"T22016"},{"id":"R15127","pred":"pobj","subj":"T22016","obj":"T22014"},{"id":"R15128","pred":"punct","subj":"T22017","obj":"T22016"},{"id":"R15129","pred":"det","subj":"T22018","obj":"T22019"},{"id":"R15130","pred":"appos","subj":"T22019","obj":"T22016"},{"id":"R15131","pred":"punct","subj":"T22020","obj":"T22016"},{"id":"R15132","pred":"punct","subj":"T22021","obj":"T22000"},{"id":"R15133","pred":"nsubj","subj":"T22022","obj":"T22027"},{"id":"R15134","pred":"prep","subj":"T22023","obj":"T22022"},{"id":"R15135","pred":"det","subj":"T22024","obj":"T22026"},{"id":"R15136","pred":"amod","subj":"T22025","obj":"T22026"},{"id":"R15137","pred":"pobj","subj":"T22026","obj":"T22023"},{"id":"R15138","pred":"ROOT","subj":"T22027","obj":"T22027"},{"id":"R15139","pred":"det","subj":"T22028","obj":"T22029"},{"id":"R15140","pred":"dobj","subj":"T22029","obj":"T22027"},{"id":"R15141","pred":"prep","subj":"T22030","obj":"T22029"},{"id":"R15142","pred":"pobj","subj":"T22031","obj":"T22030"},{"id":"R15143","pred":"cc","subj":"T22032","obj":"T22029"},{"id":"R15144","pred":"punct","subj":"T22033","obj":"T22034"},{"id":"R15145","pred":"conj","subj":"T22034","obj":"T22029"},{"id":"R15146","pred":"punct","subj":"T22035","obj":"T22034"},{"id":"R15147","pred":"conj","subj":"T22036","obj":"T22034"},{"id":"R15148","pred":"punct","subj":"T22037","obj":"T22027"},{"id":"R15149","pred":"det","subj":"T22038","obj":"T22039"},{"id":"R15150","pred":"nsubjpass","subj":"T22039","obj":"T22045"},{"id":"R15151","pred":"prep","subj":"T22040","obj":"T22039"},{"id":"R15152","pred":"det","subj":"T22041","obj":"T22043"},{"id":"R15153","pred":"compound","subj":"T22042","obj":"T22043"},{"id":"R15154","pred":"pobj","subj":"T22043","obj":"T22040"},{"id":"R15155","pred":"auxpass","subj":"T22044","obj":"T22045"},{"id":"R15156","pred":"ROOT","subj":"T22045","obj":"T22045"},{"id":"R15157","pred":"punct","subj":"T22046","obj":"T22045"},{"id":"R15158","pred":"advmod","subj":"T22047","obj":"T22049"},{"id":"R15159","pred":"punct","subj":"T22048","obj":"T22049"},{"id":"R15160","pred":"npadvmod","subj":"T22049","obj":"T22045"},{"id":"R15161","pred":"punct","subj":"T22050","obj":"T22049"},{"id":"R15162","pred":"punct","subj":"T22051","obj":"T22045"},{"id":"R15163","pred":"compound","subj":"T22052","obj":"T22055"},{"id":"R15164","pred":"punct","subj":"T22053","obj":"T22054"},{"id":"R15165","pred":"compound","subj":"T22054","obj":"T22055"},{"id":"R15166","pred":"nsubj","subj":"T22055","obj":"T22064"},{"id":"R15167","pred":"amod","subj":"T22056","obj":"T22055"},{"id":"R15168","pred":"prep","subj":"T22057","obj":"T22056"},{"id":"R15169","pred":"pobj","subj":"T22058","obj":"T22057"},{"id":"R15170","pred":"prep","subj":"T22059","obj":"T22064"},{"id":"R15171","pred":"compound","subj":"T22060","obj":"T22062"},{"id":"R15172","pred":"compound","subj":"T22061","obj":"T22062"},{"id":"R15173","pred":"pobj","subj":"T22062","obj":"T22059"},{"id":"R15174","pred":"punct","subj":"T22063","obj":"T22064"},{"id":"R15175","pred":"ROOT","subj":"T22064","obj":"T22064"},{"id":"R15176","pred":"prep","subj":"T22065","obj":"T22064"},{"id":"R15177","pred":"pobj","subj":"T22066","obj":"T22065"},{"id":"R15178","pred":"punct","subj":"T22067","obj":"T22066"},{"id":"R15179","pred":"conj","subj":"T22068","obj":"T22066"},{"id":"R15180","pred":"punct","subj":"T22069","obj":"T22068"},{"id":"R15181","pred":"cc","subj":"T22070","obj":"T22068"},{"id":"R15182","pred":"nummod","subj":"T22071","obj":"T22073"},{"id":"R15183","pred":"nummod","subj":"T22072","obj":"T22073"},{"id":"R15184","pred":"conj","subj":"T22073","obj":"T22068"},{"id":"R15185","pred":"punct","subj":"T22074","obj":"T22064"},{"id":"R15186","pred":"compound","subj":"T22075","obj":"T22076"},{"id":"R15187","pred":"nsubjpass","subj":"T22076","obj":"T22078"},{"id":"R15188","pred":"auxpass","subj":"T22077","obj":"T22078"},{"id":"R15189","pred":"ROOT","subj":"T22078","obj":"T22078"},{"id":"R15190","pred":"prep","subj":"T22079","obj":"T22078"},{"id":"R15191","pred":"compound","subj":"T22080","obj":"T22081"},{"id":"R15192","pred":"pobj","subj":"T22081","obj":"T22079"},{"id":"R15193","pred":"punct","subj":"T22082","obj":"T22081"},{"id":"R15194","pred":"conj","subj":"T22083","obj":"T22081"},{"id":"R15195","pred":"punct","subj":"T22084","obj":"T22083"},{"id":"R15196","pred":"conj","subj":"T22085","obj":"T22083"},{"id":"R15197","pred":"punct","subj":"T22086","obj":"T22085"},{"id":"R15198","pred":"conj","subj":"T22087","obj":"T22085"},{"id":"R15199","pred":"punct","subj":"T22088","obj":"T22090"},{"id":"R15200","pred":"nummod","subj":"T22089","obj":"T22090"},{"id":"R15201","pred":"appos","subj":"T22090","obj":"T22087"},{"id":"R15202","pred":"punct","subj":"T22091","obj":"T22090"},{"id":"R15203","pred":"punct","subj":"T22092","obj":"T22087"},{"id":"R15204","pred":"compound","subj":"T22093","obj":"T22095"},{"id":"R15205","pred":"compound","subj":"T22094","obj":"T22095"},{"id":"R15206","pred":"conj","subj":"T22095","obj":"T22087"},{"id":"R15207","pred":"cc","subj":"T22096","obj":"T22095"},{"id":"R15208","pred":"compound","subj":"T22097","obj":"T22099"},{"id":"R15209","pred":"compound","subj":"T22098","obj":"T22099"},{"id":"R15210","pred":"conj","subj":"T22099","obj":"T22095"},{"id":"R15211","pred":"prep","subj":"T22100","obj":"T22078"},{"id":"R15212","pred":"nummod","subj":"T22101","obj":"T22102"},{"id":"R15213","pred":"pobj","subj":"T22102","obj":"T22100"},{"id":"R15214","pred":"prep","subj":"T22103","obj":"T22102"},{"id":"R15215","pred":"nummod","subj":"T22104","obj":"T22105"},{"id":"R15216","pred":"nummod","subj":"T22105","obj":"T22106"},{"id":"R15217","pred":"pobj","subj":"T22106","obj":"T22103"},{"id":"R15218","pred":"punct","subj":"T22107","obj":"T22078"},{"id":"R15219","pred":"det","subj":"T22108","obj":"T22109"},{"id":"R15220","pred":"nsubjpass","subj":"T22109","obj":"T22115"},{"id":"R15221","pred":"prep","subj":"T22110","obj":"T22109"},{"id":"R15222","pred":"det","subj":"T22111","obj":"T22113"},{"id":"R15223","pred":"compound","subj":"T22112","obj":"T22113"},{"id":"R15224","pred":"pobj","subj":"T22113","obj":"T22110"},{"id":"R15225","pred":"auxpass","subj":"T22114","obj":"T22115"},{"id":"R15226","pred":"ROOT","subj":"T22115","obj":"T22115"},{"id":"R15227","pred":"punct","subj":"T22116","obj":"T22115"},{"id":"R15228","pred":"amod","subj":"T22117","obj":"T22118"},{"id":"R15229","pred":"ROOT","subj":"T22118","obj":"T22118"},{"id":"R15230","pred":"prep","subj":"T22119","obj":"T22118"},{"id":"R15231","pred":"pobj","subj":"T22120","obj":"T22119"},{"id":"R15232","pred":"amod","subj":"T22121","obj":"T22122"},{"id":"R15233","pred":"pobj","subj":"T22122","obj":"T22119"},{"id":"R15234","pred":"prep","subj":"T22123","obj":"T22118"},{"id":"R15235","pred":"dobj","subj":"T22124","obj":"T22123"},{"id":"R15236","pred":"punct","subj":"T22125","obj":"T22126"},{"id":"R15237","pred":"nummod","subj":"T22126","obj":"T22124"},{"id":"R15238","pred":"cc","subj":"T22127","obj":"T22124"},{"id":"R15239","pred":"nummod","subj":"T22128","obj":"T22129"},{"id":"R15240","pred":"conj","subj":"T22129","obj":"T22124"},{"id":"R15241","pred":"punct","subj":"T22130","obj":"T22129"},{"id":"R15242","pred":"cc","subj":"T22131","obj":"T22129"},{"id":"R15243","pred":"nummod","subj":"T22132","obj":"T22133"},{"id":"R15244","pred":"conj","subj":"T22133","obj":"T22129"},{"id":"R15245","pred":"punct","subj":"T22134","obj":"T22136"},{"id":"R15246","pred":"nummod","subj":"T22135","obj":"T22136"},{"id":"R15247","pred":"appos","subj":"T22136","obj":"T22133"},{"id":"R15248","pred":"punct","subj":"T22137","obj":"T22136"},{"id":"R15249","pred":"advmod","subj":"T22138","obj":"T22140"},{"id":"R15250","pred":"advmod","subj":"T22139","obj":"T22140"},{"id":"R15251","pred":"cc","subj":"T22140","obj":"T22133"},{"id":"R15252","pred":"amod","subj":"T22141","obj":"T22142"},{"id":"R15253","pred":"conj","subj":"T22142","obj":"T22133"},{"id":"R15254","pred":"prep","subj":"T22143","obj":"T22133"},{"id":"R15255","pred":"det","subj":"T22144","obj":"T22145"},{"id":"R15256","pred":"pobj","subj":"T22145","obj":"T22143"},{"id":"R15257","pred":"cc","subj":"T22146","obj":"T22145"},{"id":"R15258","pred":"det","subj":"T22147","obj":"T22149"},{"id":"R15259","pred":"amod","subj":"T22148","obj":"T22149"},{"id":"R15260","pred":"appos","subj":"T22149","obj":"T22129"},{"id":"R15261","pred":"punct","subj":"T22150","obj":"T22149"},{"id":"R15262","pred":"quantmod","subj":"T22151","obj":"T22153"},{"id":"R15263","pred":"nummod","subj":"T22152","obj":"T22153"},{"id":"R15264","pred":"appos","subj":"T22153","obj":"T22149"},{"id":"R15265","pred":"punct","subj":"T22154","obj":"T22149"},{"id":"R15266","pred":"punct","subj":"T22155","obj":"T22129"},{"id":"R15267","pred":"punct","subj":"T22156","obj":"T22118"},{"id":"R15268","pred":"compound","subj":"T22157","obj":"T22159"},{"id":"R15269","pred":"compound","subj":"T22158","obj":"T22159"},{"id":"R15270","pred":"nsubjpass","subj":"T22159","obj":"T22161"},{"id":"R15271","pred":"auxpass","subj":"T22160","obj":"T22161"},{"id":"R15272","pred":"ROOT","subj":"T22161","obj":"T22161"},{"id":"R15273","pred":"oprd","subj":"T22162","obj":"T22161"},{"id":"R15274","pred":"prep","subj":"T22163","obj":"T22161"},{"id":"R15275","pred":"nummod","subj":"T22164","obj":"T22166"},{"id":"R15276","pred":"nummod","subj":"T22165","obj":"T22166"},{"id":"R15277","pred":"pobj","subj":"T22166","obj":"T22163"},{"id":"R15278","pred":"cc","subj":"T22167","obj":"T22166"},{"id":"R15279","pred":"conj","subj":"T22168","obj":"T22166"},{"id":"R15280","pred":"punct","subj":"T22169","obj":"T22168"},{"id":"R15281","pred":"punct","subj":"T22170","obj":"T22172"},{"id":"R15282","pred":"punct","subj":"T22171","obj":"T22172"},{"id":"R15283","pred":"appos","subj":"T22172","obj":"T22168"},{"id":"R15284","pred":"nmod","subj":"T22173","obj":"T22174"},{"id":"R15285","pred":"npadvmod","subj":"T22174","obj":"T22172"},{"id":"R15286","pred":"punct","subj":"T22175","obj":"T22168"},{"id":"R15287","pred":"auxpass","subj":"T22176","obj":"T22177"},{"id":"R15288","pred":"conj","subj":"T22177","obj":"T22161"},{"id":"R15289","pred":"prep","subj":"T22178","obj":"T22177"},{"id":"R15290","pred":"pobj","subj":"T22179","obj":"T22178"},{"id":"R15291","pred":"prep","subj":"T22180","obj":"T22179"},{"id":"R15292","pred":"amod","subj":"T22181","obj":"T22182"},{"id":"R15293","pred":"pobj","subj":"T22182","obj":"T22180"},{"id":"R15294","pred":"punct","subj":"T22183","obj":"T22186"},{"id":"R15295","pred":"advmod","subj":"T22184","obj":"T22185"},{"id":"R15296","pred":"punct","subj":"T22185","obj":"T22186"},{"id":"R15297","pred":"parataxis","subj":"T22186","obj":"T22177"},{"id":"R15298","pred":"punct","subj":"T22187","obj":"T22186"},{"id":"R15299","pred":"punct","subj":"T22188","obj":"T22161"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T21552","span":{"begin":941,"end":949},"obj":"Protein"},{"id":"T21551","span":{"begin":661,"end":668},"obj":"Binding"},{"id":"T21541","span":{"begin":387,"end":395},"obj":"Protein"},{"id":"T21540","span":{"begin":283,"end":287},"obj":"Protein"},{"id":"T21539","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T21538","span":{"begin":271,"end":275},"obj":"Protein"},{"id":"T21537","span":{"begin":218,"end":243},"obj":"Protein"},{"id":"T21536","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T21535","span":{"begin":202,"end":206},"obj":"Protein"},{"id":"T21534","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T21533","span":{"begin":27,"end":34},"obj":"Binding"},{"id":"T21532","span":{"begin":27,"end":34},"obj":"Binding"},{"id":"T21531","span":{"begin":27,"end":34},"obj":"Binding"},{"id":"T21530","span":{"begin":53,"end":59},"obj":"Protein"},{"id":"T21529","span":{"begin":44,"end":48},"obj":"Protein"},{"id":"T21528","span":{"begin":38,"end":42},"obj":"Protein"},{"id":"T21550","span":{"begin":720,"end":722},"obj":"Protein"},{"id":"T21549","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T21548","span":{"begin":672,"end":676},"obj":"Protein"},{"id":"T21547","span":{"begin":557,"end":560},"obj":"Protein"},{"id":"T21546","span":{"begin":548,"end":551},"obj":"Protein"},{"id":"T21545","span":{"begin":529,"end":547},"obj":"Protein"},{"id":"T21544","span":{"begin":421,"end":442},"obj":"Protein"},{"id":"T21543","span":{"begin":407,"end":417},"obj":"Protein"},{"id":"T21542","span":{"begin":397,"end":405},"obj":"Protein"}],"relations":[{"id":"R14762","pred":"themeOf","subj":"T21528","obj":"T21531"},{"id":"R14763","pred":"themeOf","subj":"T21529","obj":"T21532"},{"id":"R14764","pred":"themeOf","subj":"T21530","obj":"T21533"},{"id":"R14765","pred":"themeOf","subj":"T21548","obj":"T21551"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"testone","denotations":[{"id":"T21413","span":{"begin":661,"end":668},"obj":"Binding"},{"id":"T21412","span":{"begin":27,"end":34},"obj":"Binding"},{"id":"T21411","span":{"begin":16,"end":19},"obj":"Positive_regulation"},{"id":"T21410","span":{"begin":998,"end":1004},"obj":"Protein"},{"id":"T21409","span":{"begin":816,"end":822},"obj":"Protein"},{"id":"T21408","span":{"begin":790,"end":796},"obj":"Protein"},{"id":"T21407","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T21406","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T21405","span":{"begin":692,"end":698},"obj":"Protein"},{"id":"T21404","span":{"begin":557,"end":560},"obj":"Protein"},{"id":"T21403","span":{"begin":548,"end":551},"obj":"Protein"},{"id":"T21402","span":{"begin":534,"end":538},"obj":"Protein"},{"id":"T21401","span":{"begin":426,"end":430},"obj":"Protein"},{"id":"T21400","span":{"begin":407,"end":413},"obj":"Protein"},{"id":"T21399","span":{"begin":387,"end":391},"obj":"Protein"},{"id":"T21398","span":{"begin":292,"end":296},"obj":"Protein"},{"id":"T21397","span":{"begin":283,"end":287},"obj":"Protein"},{"id":"T21396","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T21395","span":{"begin":271,"end":275},"obj":"Protein"},{"id":"T21394","span":{"begin":223,"end":227},"obj":"Protein"},{"id":"T21393","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T21392","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T21391","span":{"begin":53,"end":59},"obj":"Protein"},{"id":"T21390","span":{"begin":38,"end":42},"obj":"Protein"}],"relations":[{"id":"R14746","pred":"themeOf","subj":"T21390","obj":"T21412"},{"id":"R14747","pred":"themeOf","subj":"T21391","obj":"T21412"},{"id":"R14748","pred":"themeOf","subj":"T21405","obj":"T21413"},{"id":"R14749","pred":"themeOf","subj":"T21406","obj":"T21413"},{"id":"R14750","pred":"themeOf","subj":"T21407","obj":"T21413"},{"id":"R14751","pred":"themeOf","subj":"T21412","obj":"T21411"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"test3","denotations":[{"id":"T21444","span":{"begin":292,"end":296},"obj":"Protein"},{"id":"T21443","span":{"begin":283,"end":287},"obj":"Protein"},{"id":"T21442","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T21441","span":{"begin":271,"end":275},"obj":"Protein"},{"id":"T21440","span":{"begin":223,"end":227},"obj":"Protein"},{"id":"T21439","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T21438","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T21437","span":{"begin":53,"end":59},"obj":"Protein"},{"id":"T21436","span":{"begin":38,"end":42},"obj":"Protein"},{"id":"T21435","span":{"begin":27,"end":34},"obj":"Binding"},{"id":"T21434","span":{"begin":998,"end":1004},"obj":"Protein"},{"id":"T21433","span":{"begin":816,"end":822},"obj":"Protein"},{"id":"T21432","span":{"begin":790,"end":796},"obj":"Protein"},{"id":"T21431","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T21430","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T21429","span":{"begin":692,"end":698},"obj":"Protein"},{"id":"T21428","span":{"begin":557,"end":560},"obj":"Protein"},{"id":"T21427","span":{"begin":548,"end":551},"obj":"Protein"},{"id":"T21426","span":{"begin":534,"end":538},"obj":"Protein"},{"id":"T21425","span":{"begin":426,"end":430},"obj":"Protein"},{"id":"T21424","span":{"begin":407,"end":413},"obj":"Protein"},{"id":"T21423","span":{"begin":387,"end":391},"obj":"Protein"},{"id":"T21422","span":{"begin":292,"end":296},"obj":"Protein"},{"id":"T21421","span":{"begin":283,"end":287},"obj":"Protein"},{"id":"T21420","span":{"begin":277,"end":281},"obj":"Protein"},{"id":"T21419","span":{"begin":271,"end":275},"obj":"Protein"},{"id":"T21418","span":{"begin":223,"end":227},"obj":"Protein"},{"id":"T21417","span":{"begin":208,"end":214},"obj":"Protein"},{"id":"T21416","span":{"begin":196,"end":200},"obj":"Protein"},{"id":"T21415","span":{"begin":53,"end":59},"obj":"Protein"},{"id":"T21414","span":{"begin":38,"end":42},"obj":"Protein"},{"id":"T21458","span":{"begin":998,"end":1004},"obj":"Protein"},{"id":"T21457","span":{"begin":816,"end":822},"obj":"Protein"},{"id":"T21456","span":{"begin":790,"end":796},"obj":"Protein"},{"id":"T21455","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T21454","span":{"begin":712,"end":718},"obj":"Protein"},{"id":"T21453","span":{"begin":692,"end":698},"obj":"Protein"},{"id":"T21452","span":{"begin":677,"end":686},"obj":"Positive_regulation"},{"id":"T21451","span":{"begin":661,"end":668},"obj":"Binding"},{"id":"T21450","span":{"begin":557,"end":560},"obj":"Protein"},{"id":"T21449","span":{"begin":548,"end":551},"obj":"Protein"},{"id":"T21448","span":{"begin":534,"end":538},"obj":"Protein"},{"id":"T21447","span":{"begin":426,"end":430},"obj":"Protein"},{"id":"T21446","span":{"begin":407,"end":413},"obj":"Protein"},{"id":"T21445","span":{"begin":387,"end":391},"obj":"Protein"}],"relations":[{"id":"R14752","pred":"themeOf","subj":"T21436","obj":"T21435"},{"id":"R14753","pred":"themeOf","subj":"T21437","obj":"T21435"},{"id":"R14754","pred":"themeOf","subj":"T21451","obj":"T21452"},{"id":"R14755","pred":"themeOf","subj":"T21453","obj":"T21451"},{"id":"R14756","pred":"themeOf","subj":"T21454","obj":"T21451"},{"id":"R14757","pred":"themeOf","subj":"T21455","obj":"T21451"}],"text":"Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}