PMC:1472690 / 36615-37422 JSONTXT

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T19219","span":{"begin":625,"end":631},"obj":"Protein"},{"id":"T19218","span":{"begin":597,"end":603},"obj":"Protein"},{"id":"T19217","span":{"begin":571,"end":576},"obj":"Protein"},{"id":"T19216","span":{"begin":512,"end":515},"obj":"Protein"},{"id":"T19215","span":{"begin":486,"end":489},"obj":"Protein"},{"id":"T19214","span":{"begin":458,"end":461},"obj":"Protein"},{"id":"T19213","span":{"begin":352,"end":357},"obj":"Protein"},{"id":"T19212","span":{"begin":329,"end":332},"obj":"Protein"},{"id":"T19211","span":{"begin":71,"end":75},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T19367","span":{"begin":486,"end":489},"obj":"http://www.uniprot.org/uniprot/P21579"},{"id":"T19366","span":{"begin":486,"end":489},"obj":"http://www.uniprot.org/uniprot/Q04206"},{"id":"T19365","span":{"begin":458,"end":461},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T19364","span":{"begin":352,"end":357},"obj":"http://www.uniprot.org/uniprot/Q04864"},{"id":"T19363","span":{"begin":512,"end":515},"obj":"http://www.uniprot.org/uniprot/Q00653"},{"id":"T19362","span":{"begin":329,"end":332},"obj":"http://www.uniprot.org/uniprot/Q00653"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022."}

{"project":"GO-MF","denotations":[{"id":"T19236","span":{"begin":693,"end":703},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19235","span":{"begin":425,"end":435},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19234","span":{"begin":224,"end":234},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19233","span":{"begin":29,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0005515"},{"id":"T19232","span":{"begin":29,"end":36},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T19231","span":{"begin":25,"end":36},"obj":"http://purl.obolibrary.org/obo/GO_0003677"}],"text":"To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022."}

{"project":"GO-CC","denotations":[{"id":"T19252","span":{"begin":668,"end":672},"obj":"http://purl.obolibrary.org/obo/GO_0071735"},{"id":"T19251","span":{"begin":550,"end":554},"obj":"http://purl.obolibrary.org/obo/GO_0071735"},{"id":"T19248","span":{"begin":693,"end":703},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19247","span":{"begin":425,"end":435},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19246","span":{"begin":224,"end":234},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19243","span":{"begin":693,"end":703},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T19242","span":{"begin":425,"end":435},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T19241","span":{"begin":224,"end":234},"obj":"http://purl.obolibrary.org/obo/GO_0019815"}],"text":"To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022."}

{"project":"sentences","denotations":[{"id":"T19196","span":{"begin":0,"end":807},"obj":"Sentence"},{"id":"T252","span":{"begin":0,"end":807},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022."}

{"project":"simple1","denotations":[{"id":"T19274","span":{"begin":625,"end":631},"obj":"Protein"},{"id":"T19273","span":{"begin":597,"end":603},"obj":"Protein"},{"id":"T19272","span":{"begin":571,"end":576},"obj":"Protein"},{"id":"T19271","span":{"begin":512,"end":515},"obj":"Protein"},{"id":"T19270","span":{"begin":486,"end":489},"obj":"Protein"},{"id":"T19269","span":{"begin":458,"end":461},"obj":"Protein"},{"id":"T19268","span":{"begin":352,"end":357},"obj":"Protein"},{"id":"T19267","span":{"begin":329,"end":332},"obj":"Protein"},{"id":"T19266","span":{"begin":71,"end":75},"obj":"Protein"}],"text":"To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T19697","span":{"begin":625,"end":631},"obj":"Protein"},{"id":"T19696","span":{"begin":597,"end":603},"obj":"Protein"},{"id":"T19695","span":{"begin":571,"end":576},"obj":"Protein"},{"id":"T19694","span":{"begin":512,"end":515},"obj":"Protein"},{"id":"T19693","span":{"begin":486,"end":489},"obj":"Protein"},{"id":"T19692","span":{"begin":458,"end":461},"obj":"Protein"},{"id":"T19691","span":{"begin":352,"end":357},"obj":"Protein"},{"id":"T19690","span":{"begin":329,"end":332},"obj":"Protein"},{"id":"T19689","span":{"begin":71,"end":75},"obj":"Protein"}],"text":"To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T19376","span":{"begin":625,"end":631},"obj":"Protein"},{"id":"T19375","span":{"begin":597,"end":603},"obj":"Protein"},{"id":"T19374","span":{"begin":571,"end":576},"obj":"Protein"},{"id":"T19373","span":{"begin":512,"end":515},"obj":"Protein"},{"id":"T19372","span":{"begin":486,"end":489},"obj":"Protein"},{"id":"T19371","span":{"begin":458,"end":461},"obj":"Protein"},{"id":"T19370","span":{"begin":352,"end":357},"obj":"Protein"},{"id":"T19369","span":{"begin":329,"end":332},"obj":"Protein"},{"id":"T19368","span":{"begin":71,"end":75},"obj":"Protein"}],"text":"To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022."}

{"project":"DLUT931","denotations":[{"id":"T19385","span":{"begin":625,"end":631},"obj":"Protein"},{"id":"T19384","span":{"begin":597,"end":603},"obj":"Protein"},{"id":"T19383","span":{"begin":571,"end":576},"obj":"Protein"},{"id":"T19382","span":{"begin":512,"end":515},"obj":"Protein"},{"id":"T19381","span":{"begin":486,"end":489},"obj":"Protein"},{"id":"T19380","span":{"begin":458,"end":461},"obj":"Protein"},{"id":"T19379","span":{"begin":352,"end":357},"obj":"Protein"},{"id":"T19378","span":{"begin":329,"end":332},"obj":"Protein"},{"id":"T19377","span":{"begin":71,"end":75},"obj":"Protein"}],"text":"To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022."}

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