PMC:1472690 / 36597-37867 JSONTXT

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(Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T19219","span":{"begin":643,"end":649},"obj":"Protein"},{"id":"T19218","span":{"begin":615,"end":621},"obj":"Protein"},{"id":"T19217","span":{"begin":589,"end":594},"obj":"Protein"},{"id":"T19216","span":{"begin":530,"end":533},"obj":"Protein"},{"id":"T19215","span":{"begin":504,"end":507},"obj":"Protein"},{"id":"T19214","span":{"begin":476,"end":479},"obj":"Protein"},{"id":"T19213","span":{"begin":370,"end":375},"obj":"Protein"},{"id":"T19212","span":{"begin":347,"end":350},"obj":"Protein"},{"id":"T19211","span":{"begin":89,"end":93},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T19367","span":{"begin":504,"end":507},"obj":"http://www.uniprot.org/uniprot/P21579"},{"id":"T19366","span":{"begin":504,"end":507},"obj":"http://www.uniprot.org/uniprot/Q04206"},{"id":"T19365","span":{"begin":476,"end":479},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T19364","span":{"begin":370,"end":375},"obj":"http://www.uniprot.org/uniprot/Q04864"},{"id":"T19363","span":{"begin":530,"end":533},"obj":"http://www.uniprot.org/uniprot/Q00653"},{"id":"T19362","span":{"begin":347,"end":350},"obj":"http://www.uniprot.org/uniprot/Q00653"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"GO-BP","denotations":[{"id":"T19201","span":{"begin":1261,"end":1263},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19200","span":{"begin":1100,"end":1111},"obj":"http://purl.obolibrary.org/obo/GO_0016791"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"GO-MF","denotations":[{"id":"T19240","span":{"begin":1261,"end":1263},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19239","span":{"begin":1100,"end":1111},"obj":"http://purl.obolibrary.org/obo/GO_0016791"},{"id":"T19238","span":{"begin":1133,"end":1141},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19237","span":{"begin":1014,"end":1024},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19236","span":{"begin":711,"end":721},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19235","span":{"begin":443,"end":453},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19234","span":{"begin":242,"end":252},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19233","span":{"begin":47,"end":63},"obj":"http://purl.obolibrary.org/obo/GO_0005515"},{"id":"T19232","span":{"begin":47,"end":54},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T19231","span":{"begin":43,"end":54},"obj":"http://purl.obolibrary.org/obo/GO_0003677"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"GO-CC","denotations":[{"id":"T19253","span":{"begin":882,"end":890},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T19252","span":{"begin":686,"end":690},"obj":"http://purl.obolibrary.org/obo/GO_0071735"},{"id":"T19251","span":{"begin":568,"end":572},"obj":"http://purl.obolibrary.org/obo/GO_0071735"},{"id":"T19250","span":{"begin":1133,"end":1141},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19249","span":{"begin":1014,"end":1024},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19248","span":{"begin":711,"end":721},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19247","span":{"begin":443,"end":453},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19246","span":{"begin":242,"end":252},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19245","span":{"begin":1133,"end":1141},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T19244","span":{"begin":1014,"end":1024},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T19243","span":{"begin":711,"end":721},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T19242","span":{"begin":443,"end":453},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T19241","span":{"begin":242,"end":252},"obj":"http://purl.obolibrary.org/obo/GO_0019815"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"sentences","denotations":[{"id":"T19199","span":{"begin":1165,"end":1270},"obj":"Sentence"},{"id":"T19198","span":{"begin":902,"end":1164},"obj":"Sentence"},{"id":"T19197","span":{"begin":826,"end":901},"obj":"Sentence"},{"id":"T19196","span":{"begin":18,"end":825},"obj":"Sentence"},{"id":"T19195","span":{"begin":0,"end":17},"obj":"Sentence"},{"id":"T251","span":{"begin":0,"end":17},"obj":"Sentence"},{"id":"T252","span":{"begin":18,"end":825},"obj":"Sentence"},{"id":"T253","span":{"begin":826,"end":901},"obj":"Sentence"},{"id":"T254","span":{"begin":902,"end":1164},"obj":"Sentence"},{"id":"T255","span":{"begin":1165,"end":1248},"obj":"Sentence"},{"id":"T256","span":{"begin":1249,"end":1270},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"simple1","denotations":[{"id":"T19274","span":{"begin":643,"end":649},"obj":"Protein"},{"id":"T19273","span":{"begin":615,"end":621},"obj":"Protein"},{"id":"T19272","span":{"begin":589,"end":594},"obj":"Protein"},{"id":"T19271","span":{"begin":530,"end":533},"obj":"Protein"},{"id":"T19270","span":{"begin":504,"end":507},"obj":"Protein"},{"id":"T19269","span":{"begin":476,"end":479},"obj":"Protein"},{"id":"T19268","span":{"begin":370,"end":375},"obj":"Protein"},{"id":"T19267","span":{"begin":347,"end":350},"obj":"Protein"},{"id":"T19266","span":{"begin":89,"end":93},"obj":"Protein"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T19697","span":{"begin":643,"end":649},"obj":"Protein"},{"id":"T19696","span":{"begin":615,"end":621},"obj":"Protein"},{"id":"T19695","span":{"begin":589,"end":594},"obj":"Protein"},{"id":"T19694","span":{"begin":530,"end":533},"obj":"Protein"},{"id":"T19693","span":{"begin":504,"end":507},"obj":"Protein"},{"id":"T19692","span":{"begin":476,"end":479},"obj":"Protein"},{"id":"T19691","span":{"begin":370,"end":375},"obj":"Protein"},{"id":"T19690","span":{"begin":347,"end":350},"obj":"Protein"},{"id":"T19689","span":{"begin":89,"end":93},"obj":"Protein"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T19376","span":{"begin":643,"end":649},"obj":"Protein"},{"id":"T19375","span":{"begin":615,"end":621},"obj":"Protein"},{"id":"T19374","span":{"begin":589,"end":594},"obj":"Protein"},{"id":"T19373","span":{"begin":530,"end":533},"obj":"Protein"},{"id":"T19372","span":{"begin":504,"end":507},"obj":"Protein"},{"id":"T19371","span":{"begin":476,"end":479},"obj":"Protein"},{"id":"T19370","span":{"begin":370,"end":375},"obj":"Protein"},{"id":"T19369","span":{"begin":347,"end":350},"obj":"Protein"},{"id":"T19368","span":{"begin":89,"end":93},"obj":"Protein"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

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(Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T19230","span":{"begin":753,"end":756},"obj":"Protein"},{"id":"T19229","span":{"begin":670,"end":699},"obj":"Protein"},{"id":"T19228","span":{"begin":596,"end":600},"obj":"Protein"},{"id":"T19227","span":{"begin":584,"end":594},"obj":"Protein"},{"id":"T19226","span":{"begin":557,"end":582},"obj":"Protein"},{"id":"T19225","span":{"begin":525,"end":529},"obj":"Protein"},{"id":"T19224","span":{"begin":499,"end":503},"obj":"Protein"},{"id":"T19223","span":{"begin":466,"end":479},"obj":"Protein"},{"id":"T19222","span":{"begin":342,"end":350},"obj":"Protein"},{"id":"T19221","span":{"begin":89,"end":93},"obj":"Protein"},{"id":"T19220","span":{"begin":80,"end":84},"obj":"Protein"},{"id":"T19256","span":{"begin":1091,"end":1111},"obj":"Protein"},{"id":"T19255","span":{"begin":896,"end":899},"obj":"Protein"},{"id":"T19254","span":{"begin":800,"end":803},"obj":"Protein"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"testone","denotations":[{"id":"T19185","span":{"begin":643,"end":649},"obj":"Protein"},{"id":"T19184","span":{"begin":615,"end":621},"obj":"Protein"},{"id":"T19183","span":{"begin":589,"end":594},"obj":"Protein"},{"id":"T19182","span":{"begin":530,"end":533},"obj":"Protein"},{"id":"T19181","span":{"begin":504,"end":507},"obj":"Protein"},{"id":"T19180","span":{"begin":476,"end":479},"obj":"Protein"},{"id":"T19179","span":{"begin":370,"end":375},"obj":"Protein"},{"id":"T19178","span":{"begin":347,"end":350},"obj":"Protein"},{"id":"T19177","span":{"begin":89,"end":93},"obj":"Protein"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}

{"project":"test3","denotations":[{"id":"T19194","span":{"begin":643,"end":649},"obj":"Protein"},{"id":"T19193","span":{"begin":615,"end":621},"obj":"Protein"},{"id":"T19192","span":{"begin":589,"end":594},"obj":"Protein"},{"id":"T19191","span":{"begin":530,"end":533},"obj":"Protein"},{"id":"T19190","span":{"begin":504,"end":507},"obj":"Protein"},{"id":"T19189","span":{"begin":476,"end":479},"obj":"Protein"},{"id":"T19188","span":{"begin":370,"end":375},"obj":"Protein"},{"id":"T19187","span":{"begin":347,"end":350},"obj":"Protein"},{"id":"T19186","span":{"begin":89,"end":93},"obj":"Protein"}],"text":"Immuno (Dot) blot\nTo determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher \u0026 Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022. The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad). After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA). The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA)."}