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PMC:1472690 / 36597-37867 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T19361 1268-1269 -RRB- denotes )
T19360 1265-1268 NNP denotes USA
T19359 1263-1264 -COMMA- denotes ,
T19358 1261-1263 NNP denotes CA
T19357 1259-1260 -COMMA- denotes ,
T19356 1249-1259 NNP denotes Burlingame
T19355 1244-1248 NNP denotes Inc.
T19354 1231-1243 NNP denotes Laboratories
T19353 1224-1230 NNP denotes Vector
T19352 1223-1224 -LRB- denotes (
T19351 1219-1222 NN denotes kit
T19350 1210-1218 NN denotes staining
T19349 1198-1209 NNP denotes Vectastain®
T19348 1194-1197 DT denotes the
T19347 1188-1193 VB denotes using
T19346 1178-1187 VB denotes developed
T19345 1174-1177 VB denotes was
T19344 1169-1173 NN denotes blot
T19343 1165-1168 DT denotes The
T19342 1162-1163 -RRB- denotes )
T19341 1159-1162 NN denotes BSA
T19340 1157-1158 NN denotes %
T19339 1152-1157 NN denotes PBS/1
T19338 1149-1151 IN denotes in
T19337 1145-1148 CD denotes 500
T19336 1144-1145 -COLON- denotes :
T19335 1143-1144 CD denotes 1
T19334 1142-1143 -LRB- denotes (
T19333 1133-1141 NN denotes antibody
T19332 1123-1132 JJ denotes secondary
T19331 1100-1122 JJ denotes phosphatase-conjugated
T19330 1091-1099 NN denotes alkaline
T19329 1087-1090 DT denotes the
T19328 1082-1086 IN denotes with
T19327 1072-1081 VB denotes incubated
T19326 1068-1071 CC denotes and
T19325 1062-1067 RB denotes again
T19324 1055-1061 VB denotes washed
T19323 1053-1054 -COMMA- denotes ,
T19322 1050-1053 NN denotes BSA
T19321 1048-1049 NN denotes %
T19320 1043-1048 NN denotes PBS/1
T19319 1040-1042 IN denotes in
T19306 964-967 DT denotes the
T19305 962-963 -COMMA- denotes ,
T19304 961-962 -RRB- denotes )
T19303 954-961 NNP denotes Germany
T19302 952-953 -COMMA- denotes ,
T19301 941-952 NNP denotes Deisenhofen
T19300 939-940 -COMMA- denotes ,
T19299 934-939 NNP denotes Fluka
T19298 933-934 -LRB- denotes (
T19297 929-932 NN denotes BSA
T19296 927-928 NN denotes %
T19295 922-927 NN denotes PBS/3
T19294 917-921 IN denotes with
T19293 908-916 VB denotes blocking
T19292 902-907 IN denotes After
T19291 899-900 -RRB- denotes )
T19290 892-899 NN denotes Bio-Rad
T19289 891-892 -LRB- denotes (
T19288 882-890 NN denotes membrane
T19287 867-881 NN denotes nitrocellulose
T19286 865-866 DT denotes a
T19285 860-864 IN denotes onto
T19284 852-859 VB denotes blotted
T19283 847-851 VB denotes were
T19282 838-846 NN denotes proteins
T19281 830-837 JJ denotes nuclear
T19280 826-829 DT denotes The
T19279 13-17 NN denotes blot
T19278 11-12 -RRB- denotes )
T19277 8-11 NN denotes Dot
T19276 7-8 -LRB- denotes (
T19275 0-6 NN denotes Immuno
T19318 1035-1039 CD denotes 2000
T19317 1034-1035 -COLON- denotes :
T19316 1033-1034 CD denotes 1
T19315 1025-1032 VB denotes diluted
T19314 1014-1024 NN denotes antibodies
T19313 1006-1013 JJ denotes primary
T19312 1002-1005 DT denotes the
T19311 997-1001 IN denotes with
T19310 987-996 RB denotes overnight
T19309 977-986 VB denotes incubated
T19308 973-976 VB denotes was
T19307 968-972 NN denotes blot
R13198 T19288 T19285 arg2Of membrane,onto
R13199 T19288 T19286 arg1Of membrane,a
R13200 T19288 T19287 arg1Of membrane,nitrocellulose
R13201 T19288 T19289 arg1Of membrane,(
R13202 T19290 T19289 arg2Of Bio-Rad,(
R13203 T19291 T19289 arg3Of ),(
R13204 T19293 T19292 arg2Of blocking,After
R13205 T19293 T19294 arg1Of blocking,with
R13206 T19293 T19298 arg1Of blocking,(
R13207 T19297 T19294 arg2Of BSA,with
R13208 T19297 T19295 arg1Of BSA,PBS/3
R13209 T19297 T19296 arg1Of BSA,%
R13210 T19299 T19300 arg1Of Fluka,","
R13211 T19300 T19302 arg1Of ",",","
R13212 T19301 T19300 arg2Of Deisenhofen,","
R13213 T19302 T19298 arg2Of ",",(
R13214 T19303 T19302 arg2Of Germany,","
R13215 T19304 T19298 arg3Of ),(
R13216 T19307 T19293 arg1Of blot,blocking
R13217 T19307 T19306 arg1Of blot,the
R13218 T19307 T19308 arg1Of blot,was
R13219 T19307 T19309 arg2Of blot,incubated
R13220 T19309 T19292 arg1Of incubated,After
R13221 T19309 T19305 arg1Of incubated,","
R13222 T19309 T19308 arg2Of incubated,was
R13223 T19309 T19310 arg1Of incubated,overnight
R13224 T19309 T19311 arg1Of incubated,with
R13225 T19309 T19317 arg1Of incubated,:
R13226 T19314 T19311 arg2Of antibodies,with
R13227 T19314 T19312 arg1Of antibodies,the
R13228 T19314 T19313 arg1Of antibodies,primary
R13229 T19314 T19315 arg2Of antibodies,diluted
R13230 T19316 T19315 arg3Of 1,diluted
R13231 T19318 T19319 arg1Of 2000,in
R13232 T19318 T19324 arg1Of 2000,washed
R13233 T19318 T19327 arg2Of 2000,incubated
R13234 T19322 T19319 arg2Of BSA,in
R13235 T19322 T19320 arg1Of BSA,PBS/1
R13236 T19322 T19321 arg1Of BSA,%
R13237 T19324 T19325 arg1Of washed,again
R13238 T19324 T19326 arg1Of washed,and
R13239 T19326 T19317 arg2Of and,:
R13240 T19326 T19323 arg1Of and,","
R13241 T19327 T19326 arg2Of incubated,and
R13242 T19327 T19328 arg1Of incubated,with
R13243 T19333 T19328 arg2Of antibody,with
R13244 T19333 T19329 arg1Of antibody,the
R13245 T19333 T19330 arg1Of antibody,alkaline
R13246 T19333 T19331 arg1Of antibody,phosphatase-conjugated
R13247 T19333 T19332 arg1Of antibody,secondary
R13248 T19333 T19334 arg1Of antibody,(
R13249 T19335 T19336 arg1Of 1,:
R13250 T19336 T19334 arg2Of :,(
R13251 T19336 T19338 arg1Of :,in
R13252 T19337 T19336 arg2Of 500,:
R13253 T19341 T19338 arg2Of BSA,in
R13254 T19341 T19339 arg1Of BSA,PBS/1
R13255 T19341 T19340 arg1Of BSA,%
R13256 T19342 T19334 arg3Of ),(
R13257 T19344 T19343 arg1Of blot,The
R13258 T19344 T19345 arg1Of blot,was
R13259 T19344 T19346 arg2Of blot,developed
R13260 T19346 T19345 arg2Of developed,was
R13261 T19347 T19346 arg3Of using,developed
R13262 T19351 T19347 arg2Of kit,using
R13263 T19351 T19348 arg1Of kit,the
R13264 T19351 T19349 arg1Of kit,Vectastain®
R13265 T19351 T19350 arg1Of kit,staining
R13266 T19351 T19352 arg1Of kit,(
R13267 T19356 T19352 arg2Of Burlingame,(
R13268 T19356 T19353 arg1Of Burlingame,Vector
R13269 T19356 T19354 arg1Of Burlingame,Laboratories
R13270 T19356 T19355 arg1Of Burlingame,Inc.
R13271 T19356 T19357 arg1Of Burlingame,","
R13272 T19358 T19357 arg2Of CA,","
R13273 T19358 T19359 arg1Of CA,","
R13274 T19360 T19359 arg2Of USA,","
R13275 T19361 T19352 arg3Of ),(
R13188 T19275 T19276 arg1Of Immuno,(
R13189 T19277 T19276 arg2Of Dot,(
R13190 T19278 T19276 arg3Of ),(
R13191 T19279 T19275 arg1Of blot,Immuno
R13192 T19282 T19280 arg1Of proteins,The
R13193 T19282 T19281 arg1Of proteins,nuclear
R13194 T19282 T19283 arg1Of proteins,were
R13195 T19282 T19284 arg2Of proteins,blotted
R13196 T19284 T19283 arg2Of blotted,were
R13197 T19284 T19285 arg1Of blotted,onto

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T19219 643-649 Protein denotes NFATc1
T19218 615-621 Protein denotes NFATc2
T19217 589-594 Protein denotes c-rel
T19216 530-533 Protein denotes p52
T19215 504-507 Protein denotes p65
T19214 476-479 Protein denotes p50
T19213 370-375 Protein denotes c-Rel
T19212 347-350 Protein denotes p52
T19211 89-93 Protein denotes NFAT

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T19367 504-507 http://www.uniprot.org/uniprot/P21579 denotes p65
T19366 504-507 http://www.uniprot.org/uniprot/Q04206 denotes p65
T19365 476-479 http://www.uniprot.org/uniprot/P19838 denotes p50
T19364 370-375 http://www.uniprot.org/uniprot/Q04864 denotes c-Rel
T19363 530-533 http://www.uniprot.org/uniprot/Q00653 denotes p52
T19362 347-350 http://www.uniprot.org/uniprot/Q00653 denotes p52

GO-BP

Id Subject Object Predicate Lexical cue
T19201 1261-1263 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T19200 1100-1111 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-MF

Id Subject Object Predicate Lexical cue
T19240 1261-1263 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T19239 1100-1111 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T19238 1133-1141 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T19237 1014-1024 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T19236 711-721 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T19235 443-453 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T19234 242-252 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T19233 47-63 http://purl.obolibrary.org/obo/GO_0005515 denotes binding proteins
T19232 47-54 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T19231 43-54 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding

GO-CC

Id Subject Object Predicate Lexical cue
T19253 882-890 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T19252 686-690 http://purl.obolibrary.org/obo/GO_0071735 denotes IgG1
T19251 568-572 http://purl.obolibrary.org/obo/GO_0071735 denotes IgG1
T19250 1133-1141 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T19249 1014-1024 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T19248 711-721 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T19247 443-453 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T19246 242-252 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T19245 1133-1141 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T19244 1014-1024 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T19243 711-721 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T19242 443-453 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T19241 242-252 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T19199 1165-1270 Sentence denotes The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA).
T19198 902-1164 Sentence denotes After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA).
T19197 826-901 Sentence denotes The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad).
T19196 18-825 Sentence denotes To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher & Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022.
T19195 0-17 Sentence denotes Immuno (Dot) blot
T251 0-17 Sentence denotes Immuno (Dot) blot
T252 18-825 Sentence denotes To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher & Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022.
T253 826-901 Sentence denotes The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad).
T254 902-1164 Sentence denotes After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA).
T255 1165-1248 Sentence denotes The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc.
T256 1249-1270 Sentence denotes Burlingame, CA, USA).

simple1

Id Subject Object Predicate Lexical cue
T19274 643-649 Protein denotes NFATc1
T19273 615-621 Protein denotes NFATc2
T19272 589-594 Protein denotes c-rel
T19271 530-533 Protein denotes p52
T19270 504-507 Protein denotes p65
T19269 476-479 Protein denotes p50
T19268 370-375 Protein denotes c-Rel
T19267 347-350 Protein denotes p52
T19266 89-93 Protein denotes NFAT

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T19697 643-649 Protein denotes NFATc1
T19696 615-621 Protein denotes NFATc2
T19695 589-594 Protein denotes c-rel
T19694 530-533 Protein denotes p52
T19693 504-507 Protein denotes p65
T19692 476-479 Protein denotes p50
T19691 370-375 Protein denotes c-Rel
T19690 347-350 Protein denotes p52
T19689 89-93 Protein denotes NFAT

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T19376 643-649 Protein denotes NFATc1
T19375 615-621 Protein denotes NFATc2
T19374 589-594 Protein denotes c-rel
T19373 530-533 Protein denotes p52
T19372 504-507 Protein denotes p65
T19371 476-479 Protein denotes p50
T19370 370-375 Protein denotes c-Rel
T19369 347-350 Protein denotes p52
T19368 89-93 Protein denotes NFAT

DLUT931

Id Subject Object Predicate Lexical cue
T19385 643-649 Protein denotes NFATc1
T19384 615-621 Protein denotes NFATc2
T19383 589-594 Protein denotes c-rel
T19382 530-533 Protein denotes p52
T19381 504-507 Protein denotes p65
T19380 476-479 Protein denotes p50
T19379 370-375 Protein denotes c-Rel
T19378 347-350 Protein denotes p52
T19377 89-93 Protein denotes NFAT

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T19688 512-519 Entity denotes sc-109,
T19687 370-383 Protein denotes c-Rel (61 kD)
T19686 757-772 Entity denotes AP-conjugate sc
T19685 419-433 Entity denotes PMA-stimulated
T19684 242-252 Entity denotes antibodies
T19683 39-63 Protein denotes the DNA binding proteins
T19682 615-628 Protein denotes NFATc2 (M-20)
T19681 494-511 Protein denotes anti-NFκB p65 (A)
T19680 589-601 Protein denotes c-rel (N466)
T19679 1152-1162 Protein denotes PBS/1% BSA
T19678 666-699 Protein denotes two goat polyclonal IgG1 antisera
T19677 466-485 Protein denotes anti-NFκB p50 (NLS)
T19676 596-600 Entity denotes N466
T19675 826-846 Protein denotes The nuclear proteins
T19674 1043-1054 Protein denotes PBS/1% BSA,
T19673 113-116 Protein denotes SRC
T19672 395-417 Entity denotes Jurkat nuclear extract
T19671 584-604 Entity denotes anti-c-rel (N466) sc
T19670 80-84 Protein denotes NFκB
T19669 701-734 Entity denotes secondary antibodies (0.8 μg/dot)
T19668 632-649 Protein denotes 1151, anti-NFATc1
T19667 651-655 Protein denotes K-18
T19666 111-132 Protein denotes a SRC96D SNS minifold
T19665 89-94 Protein denotes NFAT,
T19664 1014-1024 Entity denotes antibodies
T19663 435-464 Entity denotes primary antibodies (1 μg/dot)
T19662 605-631 Entity denotes 272, anti-NFATc2 (M-20) sc
T19661 548-582 Protein denotes a rabbit polyclonal IgG1 antiserum
T19660 922-932 Protein denotes PBS/3% BSA
T19659 757-772 Entity denotes AP-conjugate sc
T19658 512-542 Entity denotes sc-109, anti-NFκB p52 (447) sc
T19657 347-358 Protein denotes p52 (80 kD)
T19656 1087-1141 Entity denotes the alkaline phosphatase-conjugated secondary antibody
T19655 530-539 Protein denotes p52 (447)
T19654 342-361 Protein denotes NFκB p52 (80 kD) sc

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T19639 1269-1270 . denotes .
T19638 1244-1248 NNP denotes Inc.
T19637 1231-1243 NNPS denotes Laboratories
T19636 1224-1230 NNP denotes Vector
T19635 1223-1224 -LRB- denotes (
T19634 1219-1222 NN denotes kit
T19633 1210-1218 VBG denotes staining
T19632 1208-1209 NN denotes ®
T19631 1198-1208 NNP denotes Vectastain
T19630 1194-1197 DT denotes the
T19629 1188-1193 VBG denotes using
T19628 1178-1187 VBN denotes developed
T19627 1174-1177 VBD denotes was
T19626 1169-1173 NN denotes blot
T19625 1165-1168 DT denotes The
T19624 1163-1164 . denotes .
T19623 1162-1163 -RRB- denotes )
T19622 1159-1162 NNP denotes BSA
T19621 1157-1158 NN denotes %
T19620 1152-1157 CD denotes PBS/1
T19619 1149-1151 IN denotes in
T19618 1143-1148 CD denotes 1:500
T19617 1142-1143 -LRB- denotes (
T19616 1133-1141 NN denotes antibody
T19615 1123-1132 JJ denotes secondary
T19614 1100-1122 JJ denotes phosphatase-conjugated
T19613 1091-1099 JJ denotes alkaline
T19612 1087-1090 DT denotes the
T19611 1082-1086 IN denotes with
T19610 1072-1081 VBN denotes incubated
T19609 1068-1071 CC denotes and
T19569 860-864 IN denotes onto
T19568 852-859 VBN denotes blotted
T19567 847-851 VBD denotes were
T19566 838-846 NNS denotes proteins
T19565 830-837 JJ denotes nuclear
T19564 826-829 DT denotes The
T19563 824-825 . denotes .
T19562 817-824 NN denotes sc-2022
T19561 804-816 NNP denotes AP-conjugate
T19560 800-803 NNP denotes IgG
T19559 790-799 JJ denotes anti-goat
T19558 783-789 JJ denotes donkey
T19557 779-782 CC denotes and
T19556 777-778 , denotes ,
T19555 770-777 JJ denotes sc-2007
T19554 757-769 NNP denotes AP-conjugate
T19553 753-756 NNP denotes IgG
T19552 741-752 JJ denotes anti-rabbit
T19551 736-740 VB denotes goat
T19550 734-735 : denotes :
T19549 733-734 -RRB- denotes )
T19548 730-733 VBP denotes dot
T19547 729-730 NN denotes /
T19546 727-729 NN denotes μg
T19545 723-726 CD denotes 0.8
T19544 722-723 -LRB- denotes (
T19543 711-721 NNS denotes antibodies
T19542 701-710 JJ denotes secondary
T19541 699-700 : denotes ;
T19540 691-699 NN denotes antisera
T19539 686-690 JJ denotes IgG1
T19538 675-685 JJ denotes polyclonal
T19537 670-674 NN denotes goat
T19536 666-669 CD denotes two
T19535 664-665 , denotes ,
T19534 657-664 JJ denotes sc-1149
T19533 655-656 -RRB- denotes )
T19532 651-655 NN denotes K-18
T19531 650-651 -LRB- denotes (
T19530 638-649 JJ denotes anti-NFATc1
T19529 636-637 , denotes ,
T19528 629-636 JJ denotes sc-1151
T19527 627-628 -RRB- denotes )
T19526 623-627 NN denotes M-20
T19525 622-623 -LRB- denotes (
T19524 610-621 JJ denotes anti-NFATc2
T19523 608-609 , denotes ,
T19522 602-608 JJ denotes sc-272
T19521 600-601 -RRB- denotes )
T19520 596-600 NNP denotes N466
T19519 595-596 -LRB- denotes (
T19518 584-594 JJ denotes anti-c-rel
T19517 582-583 , denotes ,
T19516 573-582 NN denotes antiserum
T19515 568-572 JJ denotes IgG1
T19514 557-567 NN denotes polyclonal
T19513 550-556 NN denotes rabbit
T19512 548-549 DT denotes a
T19511 546-547 , denotes ,
T19510 540-546 JJ denotes sc-848
T19509 538-539 -RRB- denotes )
T19508 535-538 CD denotes 447
T19507 534-535 -LRB- denotes (
T19506 530-533 NN denotes p52
T19505 520-529 JJ denotes anti-NFκB
T19504 518-519 , denotes ,
T19503 512-518 JJ denotes sc-109
T19502 510-511 -RRB- denotes )
T19501 509-510 NNP denotes A
T19500 508-509 -LRB- denotes (
T19499 504-507 NNS denotes p65
T19498 494-503 JJ denotes anti-NFκB
T19497 492-493 , denotes ,
T19496 486-492 JJ denotes sc-114
T19495 484-485 -RRB- denotes )
T19494 481-484 NNP denotes NLS
T19493 480-481 -LRB- denotes (
T19492 476-479 JJ denotes p50
T19491 466-475 JJ denotes anti-NFκB
T19490 464-465 : denotes :
T19489 463-464 -RRB- denotes )
T19488 460-463 VBP denotes dot
T19487 459-460 NN denotes /
T19486 457-459 NN denotes μg
T19485 455-456 CD denotes 1
T19484 454-455 -LRB- denotes (
T19483 443-453 NNS denotes antibodies
T19482 435-442 JJ denotes primary
T19481 433-434 : denotes ;
T19480 419-433 JJ denotes PMA-stimulated
T19479 417-418 , denotes ,
T19478 410-417 VB denotes extract
T19477 402-409 JJ denotes nuclear
T19476 395-401 NNP denotes Jurkat
T19475 393-394 : denotes ;
T19474 384-393 NN denotes sc-4030WB
T19473 382-383 -RRB- denotes )
T19472 380-382 NN denotes kD
T19471 377-379 CD denotes 61
T19470 376-377 -LRB- denotes (
T19469 370-375 NNP denotes c-Rel
T19468 368-369 , denotes ,
T19467 359-368 NN denotes sc-4095WB
T19466 357-358 -RRB- denotes )
T19465 355-357 NNP denotes kD
T19464 352-354 CD denotes 80
T19463 351-352 -LRB- denotes (
T19462 347-350 CD denotes p52
T19461 342-346 NNP denotes NFκB
T19460 340-341 : denotes :
T19459 339-340 -RRB- denotes )
T19458 333-339 NN denotes ng/dot
T19457 330-332 CD denotes 10
T19456 329-330 -LRB- denotes (
T19455 320-328 NNS denotes controls
T19454 311-319 JJ denotes positive
T19453 309-310 : denotes :
T19452 308-309 -RRB- denotes )
T19451 301-308 NNP denotes Germany
T19450 299-300 , denotes ,
T19449 289-299 NNP denotes Heidelberg
T19448 287-288 , denotes ,
T19447 274-287 NNP denotes Biotechnology
T19446 269-273 NNP denotes Cruz
T19445 263-268 NNP denotes Santa
T19444 258-262 IN denotes from
T19443 254-257 DT denotes all
T19442 253-254 -LRB- denotes (
T19441 242-252 NNS denotes antibodies
T19440 238-241 CC denotes and
T19439 229-237 NNS denotes controls
T19438 220-228 JJ denotes positive
T19437 210-219 VBG denotes following
T19436 206-209 DT denotes the
T19435 201-205 IN denotes with
T19434 191-200 VBN denotes performed
T19433 186-190 VBD denotes were
T19432 184-185 -RRB- denotes )
T19431 177-184 NNP denotes Germany
T19430 175-176 , denotes ,
T19429 169-175 NNP denotes Dassel
T19428 167-168 , denotes ,
T19427 161-167 NNP denotes Schüll
T19426 159-160 CC denotes &
T19425 148-158 NNP denotes Schleicher
T19424 147-148 -LRB- denotes (
T19423 139-146 NN denotes blotter
T19422 135-138 VBP denotes dot
T19421 133-134 PRP denotes I
T19420 124-132 NN denotes minifold
T19419 120-123 NNP denotes SNS
T19418 113-119 NNP denotes SRC96D
T19417 111-112 DT denotes a
T19416 105-110 VBG denotes using
T19415 99-104 NNS denotes blots
T19414 95-98 VBP denotes dot
T19413 93-94 , denotes ,
T19412 89-93 NNP denotes NFAT
T19411 85-88 CC denotes and
T19410 80-84 NNP denotes NFκB
T19409 77-79 TO denotes to
T19408 69-76 VBN denotes related
T19407 64-68 VBD denotes were
T19406 55-63 NNS denotes proteins
T19405 47-54 JJ denotes binding
T19404 43-46 NNP denotes DNA
T19403 39-42 DT denotes the
T19402 31-38 IN denotes whether
T19401 21-30 VB denotes determine
T19400 18-20 TO denotes To
T19399 13-17 NN denotes blot
T19398 11-12 -RRB- denotes )
T19397 8-11 NNP denotes Dot
T19396 7-8 -LRB- denotes (
T19395 0-6 NNP denotes Immuno
T19608 1062-1067 RB denotes again
T19607 1055-1061 VBD denotes washed
T19606 1053-1054 , denotes ,
T19605 1050-1053 NNP denotes BSA
T19604 1048-1049 NN denotes %
T19603 1043-1048 CD denotes PBS/1
T19602 1040-1042 IN denotes in
T19601 1033-1039 CD denotes 1:2000
T19600 1025-1032 VBN denotes diluted
T19599 1014-1024 NNS denotes antibodies
T19598 1006-1013 JJ denotes primary
T19597 1002-1005 DT denotes the
T19596 997-1001 IN denotes with
T19595 987-996 JJ denotes overnight
T19594 977-986 VBN denotes incubated
T19593 973-976 VBD denotes was
T19592 968-972 NN denotes blot
T19591 964-967 DT denotes the
T19590 962-963 , denotes ,
T19589 961-962 -RRB- denotes )
T19588 954-961 NNP denotes Germany
T19587 952-953 , denotes ,
T19586 941-952 NNP denotes Deisenhofen
T19585 939-940 , denotes ,
T19584 934-939 NNP denotes Fluka
T19583 933-934 -LRB- denotes (
T19582 929-932 NNP denotes BSA
T19581 927-928 NN denotes %
T19580 922-927 CD denotes PBS/3
T19579 917-921 IN denotes with
T19578 908-916 VBG denotes blocking
T19577 902-907 IN denotes After
T19576 900-901 . denotes .
T19575 899-900 -RRB- denotes )
T19574 892-899 NNP denotes Bio-Rad
T19573 891-892 -LRB- denotes (
T19572 882-890 NN denotes membrane
T19571 867-881 JJ denotes nitrocellulose
T19570 865-866 DT denotes a
R13277 T19396 T19399 punct (,blot
R13278 T19397 T19399 nmod Dot,blot
R13279 T19398 T19399 punct ),blot
R13280 T19399 T19395 appos blot,Immuno
R13281 T19400 T19401 aux To,determine
R13282 T19401 T19478 ccomp determine,extract
R13283 T19402 T19408 mark whether,related
R13284 T19403 T19406 det the,proteins
R13285 T19404 T19406 nmod DNA,proteins
R13286 T19405 T19406 amod binding,proteins
R13287 T19406 T19408 nsubjpass proteins,related
R13288 T19407 T19408 auxpass were,related
R13289 T19408 T19401 ccomp related,determine
R13290 T19409 T19408 prep to,related
R13291 T19410 T19409 pobj NFκB,to
R13292 T19411 T19410 cc and,NFκB
R13293 T19412 T19410 conj NFAT,NFκB
R13294 T19413 T19401 punct ",",determine
R13295 T19414 T19478 ccomp dot,extract
R13296 T19415 T19414 dobj blots,dot
R13297 T19416 T19414 advcl using,dot
R13298 T19417 T19420 det a,minifold
R13299 T19418 T19420 compound SRC96D,minifold
R13300 T19419 T19420 compound SNS,minifold
R13301 T19420 T19416 dobj minifold,using
R13302 T19421 T19422 nsubj I,dot
R13303 T19422 T19478 ccomp dot,extract
R13304 T19423 T19422 dobj blotter,dot
R13305 T19424 T19434 punct (,performed
R13306 T19425 T19434 nsubjpass Schleicher,performed
R13307 T19426 T19425 cc &,Schleicher
R13308 T19427 T19425 conj Schüll,Schleicher
R13309 T19428 T19427 punct ",",Schüll
R13310 T19429 T19427 conj Dassel,Schüll
R13311 T19430 T19429 punct ",",Dassel
R13312 T19431 T19429 conj Germany,Dassel
R13313 T19432 T19434 punct ),performed
R13314 T19433 T19434 auxpass were,performed
R13315 T19434 T19478 ccomp performed,extract
R13316 T19435 T19434 prep with,performed
R13317 T19436 T19439 det the,controls
R13318 T19437 T19439 amod following,controls
R13319 T19438 T19439 amod positive,controls
R13320 T19439 T19435 pobj controls,with
R13321 T19440 T19439 cc and,controls
R13322 T19441 T19439 conj antibodies,controls
R13323 T19442 T19439 punct (,controls
R13324 T19443 T19444 advmod all,from
R13325 T19444 T19439 prep from,controls
R13326 T19445 T19446 compound Santa,Cruz
R13327 T19446 T19447 compound Cruz,Biotechnology
R13328 T19447 T19444 pobj Biotechnology,from
R13329 T19448 T19447 punct ",",Biotechnology
R13330 T19449 T19447 conj Heidelberg,Biotechnology
R13331 T19450 T19449 punct ",",Heidelberg
R13332 T19451 T19447 appos Germany,Biotechnology
R13333 T19452 T19439 punct ),controls
R13334 T19453 T19439 punct :,controls
R13335 T19454 T19455 amod positive,controls
R13336 T19455 T19439 appos controls,controls
R13337 T19456 T19455 punct (,controls
R13338 T19457 T19458 nummod 10,ng/dot
R13339 T19458 T19455 appos ng/dot,controls
R13340 T19459 T19455 punct ),controls
R13341 T19460 T19439 punct :,controls
R13342 T19461 T19462 compound NFκB,p52
R13343 T19462 T19439 appos p52,controls
R13344 T19463 T19465 punct (,kD
R13345 T19464 T19465 nummod 80,kD
R13346 T19465 T19462 appos kD,p52
R13347 T19466 T19439 punct ),controls
R13348 T19467 T19434 dobj sc-4095WB,performed
R13349 T19468 T19467 punct ",",sc-4095WB
R13350 T19469 T19467 appos c-Rel,sc-4095WB
R13351 T19470 T19472 punct (,kD
R13352 T19471 T19472 nummod 61,kD
R13353 T19472 T19469 appos kD,c-Rel
R13354 T19473 T19472 punct ),kD
R13355 T19474 T19469 amod sc-4030WB,c-Rel
R13356 T19475 T19478 punct ;,extract
R13357 T19476 T19478 nsubj Jurkat,extract
R13358 T19477 T19478 amod nuclear,extract
R13359 T19478 T19478 ROOT extract,extract
R13360 T19479 T19478 punct ",",extract
R13361 T19480 T19478 dep PMA-stimulated,extract
R13362 T19481 T19478 punct ;,extract
R13363 T19482 T19483 amod primary,antibodies
R13364 T19483 T19478 dobj antibodies,extract
R13365 T19484 T19483 punct (,antibodies
R13366 T19485 T19486 nummod 1,μg
R13367 T19486 T19488 compound μg,dot
R13368 T19487 T19488 compound /,dot
R13369 T19488 T19483 appos dot,antibodies
R13370 T19489 T19483 punct ),antibodies
R13371 T19490 T19483 punct :,antibodies
R13372 T19491 T19492 compound anti-NFκB,p50
R13373 T19492 T19506 nmod p50,p52
R13374 T19493 T19494 punct (,NLS
R13375 T19494 T19492 appos NLS,p50
R13376 T19495 T19494 punct ),NLS
R13377 T19496 T19499 amod sc-114,p65
R13378 T19497 T19499 punct ",",p65
R13379 T19498 T19499 amod anti-NFκB,p65
R13380 T19499 T19492 appos p65,p50
R13381 T19500 T19499 punct (,p65
R13382 T19501 T19499 appos A,p65
R13383 T19502 T19499 punct ),p65
R13384 T19503 T19506 nmod sc-109,p52
R13385 T19504 T19503 punct ",",sc-109
R13386 T19505 T19506 amod anti-NFκB,p52
R13387 T19506 T19483 appos p52,antibodies
R13388 T19507 T19506 punct (,p52
R13389 T19508 T19506 appos 447,p52
R13390 T19509 T19506 punct ),p52
R13391 T19510 T19506 conj sc-848,p52
R13392 T19511 T19510 punct ",",sc-848
R13393 T19512 T19516 det a,antiserum
R13394 T19513 T19516 nmod rabbit,antiserum
R13395 T19514 T19516 nmod polyclonal,antiserum
R13396 T19515 T19516 amod IgG1,antiserum
R13397 T19516 T19510 conj antiserum,sc-848
R13398 T19517 T19516 punct ",",antiserum
R13399 T19518 T19516 conj anti-c-rel,antiserum
R13400 T19519 T19518 punct (,anti-c-rel
R13401 T19520 T19518 appos N466,anti-c-rel
R13402 T19521 T19518 punct ),anti-c-rel
R13403 T19522 T19516 conj sc-272,antiserum
R13404 T19523 T19522 punct ",",sc-272
R13405 T19524 T19522 conj anti-NFATc2,sc-272
R13406 T19525 T19524 punct (,anti-NFATc2
R13407 T19526 T19524 appos M-20,anti-NFATc2
R13408 T19527 T19524 punct ),anti-NFATc2
R13409 T19528 T19540 nmod sc-1151,antisera
R13410 T19529 T19528 punct ",",sc-1151
R13411 T19530 T19528 conj anti-NFATc1,sc-1151
R13412 T19531 T19530 punct (,anti-NFATc1
R13413 T19532 T19530 appos K-18,anti-NFATc1
R13414 T19533 T19530 punct ),anti-NFATc1
R13415 T19534 T19528 conj sc-1149,sc-1151
R13416 T19535 T19534 punct ",",sc-1149
R13417 T19536 T19540 nummod two,antisera
R13418 T19537 T19540 nmod goat,antisera
R13419 T19538 T19540 nmod polyclonal,antisera
R13420 T19539 T19540 amod IgG1,antisera
R13421 T19540 T19522 dep antisera,sc-272
R13422 T19541 T19483 punct ;,antibodies
R13423 T19542 T19543 amod secondary,antibodies
R13424 T19543 T19483 appos antibodies,antibodies
R13425 T19544 T19548 punct (,dot
R13426 T19545 T19546 nummod 0.8,μg
R13427 T19546 T19548 compound μg,dot
R13428 T19547 T19548 compound /,dot
R13429 T19548 T19543 appos dot,antibodies
R13430 T19549 T19548 punct ),dot
R13431 T19550 T19543 punct :,antibodies
R13432 T19551 T19552 compound goat,anti-rabbit
R13433 T19552 T19555 compound anti-rabbit,sc-2007
R13434 T19553 T19554 compound IgG,AP-conjugate
R13435 T19554 T19555 compound AP-conjugate,sc-2007
R13436 T19555 T19543 appos sc-2007,antibodies
R13437 T19556 T19555 punct ",",sc-2007
R13438 T19557 T19555 cc and,sc-2007
R13439 T19558 T19559 amod donkey,anti-goat
R13440 T19559 T19562 amod anti-goat,sc-2022
R13441 T19560 T19561 compound IgG,AP-conjugate
R13442 T19561 T19562 compound AP-conjugate,sc-2022
R13443 T19562 T19555 conj sc-2022,sc-2007
R13444 T19563 T19478 punct .,extract
R13445 T19564 T19566 det The,proteins
R13446 T19565 T19566 amod nuclear,proteins
R13447 T19566 T19568 nsubjpass proteins,blotted
R13448 T19567 T19568 auxpass were,blotted
R13449 T19568 T19568 ROOT blotted,blotted
R13450 T19569 T19568 prep onto,blotted
R13451 T19570 T19572 det a,membrane
R13452 T19571 T19572 amod nitrocellulose,membrane
R13453 T19572 T19569 pobj membrane,onto
R13454 T19573 T19574 punct (,Bio-Rad
R13455 T19574 T19572 appos Bio-Rad,membrane
R13456 T19575 T19568 punct ),blotted
R13457 T19576 T19568 punct .,blotted
R13458 T19577 T19594 prep After,incubated
R13459 T19578 T19577 pcomp blocking,After
R13460 T19579 T19578 prep with,blocking
R13461 T19580 T19581 nummod PBS/3,%
R13462 T19581 T19584 nmod %,Fluka
R13463 T19582 T19584 nmod BSA,Fluka
R13464 T19583 T19584 punct (,Fluka
R13465 T19584 T19579 pobj Fluka,with
R13466 T19585 T19584 punct ",",Fluka
R13467 T19586 T19584 conj Deisenhofen,Fluka
R13468 T19587 T19586 punct ",",Deisenhofen
R13469 T19588 T19586 conj Germany,Deisenhofen
R13470 T19589 T19584 punct ),Fluka
R13471 T19590 T19594 punct ",",incubated
R13472 T19591 T19592 det the,blot
R13473 T19592 T19594 nsubjpass blot,incubated
R13474 T19593 T19594 auxpass was,incubated
R13475 T19594 T19594 ROOT incubated,incubated
R13476 T19595 T19594 advmod overnight,incubated
R13477 T19596 T19594 prep with,incubated
R13478 T19597 T19599 det the,antibodies
R13479 T19598 T19599 amod primary,antibodies
R13480 T19599 T19596 pobj antibodies,with
R13481 T19600 T19599 acl diluted,antibodies
R13482 T19601 T19600 npadvmod 1:2000,diluted
R13483 T19602 T19601 prep in,1:2000
R13484 T19603 T19604 nummod PBS/1,%
R13485 T19604 T19602 pobj %,in
R13486 T19605 T19600 npadvmod BSA,diluted
R13487 T19606 T19607 punct ",",washed
R13488 T19607 T19594 dep washed,incubated
R13489 T19608 T19607 advmod again,washed
R13490 T19609 T19607 cc and,washed
R13491 T19610 T19607 conj incubated,washed
R13492 T19611 T19610 prep with,incubated
R13493 T19612 T19616 det the,antibody
R13494 T19613 T19616 amod alkaline,antibody
R13495 T19614 T19616 amod phosphatase-conjugated,antibody
R13496 T19615 T19616 amod secondary,antibody
R13497 T19616 T19611 pobj antibody,with
R13498 T19617 T19616 punct (,antibody
R13499 T19618 T19616 appos 1:500,antibody
R13500 T19619 T19618 prep in,1:500
R13501 T19620 T19621 nummod PBS/1,%
R13502 T19621 T19622 compound %,BSA
R13503 T19622 T19619 pobj BSA,in
R13504 T19623 T19616 punct ),antibody
R13505 T19624 T19594 punct .,incubated
R13506 T19625 T19626 det The,blot
R13507 T19626 T19628 nsubjpass blot,developed
R13508 T19627 T19628 auxpass was,developed
R13509 T19628 T19628 ROOT developed,developed
R13510 T19629 T19628 advcl using,developed
R13511 T19630 T19632 det the,®
R13512 T19631 T19632 compound Vectastain,®
R13513 T19632 T19629 dobj ®,using
R13514 T19633 T19629 conj staining,using
R13515 T19634 T19633 dobj kit,staining
R13516 T19635 T19634 punct (,kit
R13517 T19636 T19638 compound Vector,Inc.
R13518 T19637 T19638 compound Laboratories,Inc.
R13519 T19638 T19634 appos Inc.,kit
R13520 T19639 T19628 punct .,developed
R13276 T19395 T19401 nsubj Immuno,determine

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T19230 753-756 Protein denotes IgG
T19229 670-699 Protein denotes goat polyclonal IgG1 antisera
T19228 596-600 Protein denotes N466
T19227 584-594 Protein denotes anti-c-rel
T19226 557-582 Protein denotes polyclonal IgG1 antiserum
T19225 525-529 Protein denotes NFκB
T19224 499-503 Protein denotes NFκB
T19223 466-479 Protein denotes anti-NFκB p50
T19222 342-350 Protein denotes NFκB p52
T19221 89-93 Protein denotes NFAT
T19220 80-84 Protein denotes NFκB
T19256 1091-1111 Protein denotes alkaline phosphatase
T19255 896-899 Protein denotes Rad
T19254 800-803 Protein denotes IgG

testone

Id Subject Object Predicate Lexical cue
T19185 643-649 Protein denotes NFATc1
T19184 615-621 Protein denotes NFATc2
T19183 589-594 Protein denotes c-rel
T19182 530-533 Protein denotes p52
T19181 504-507 Protein denotes p65
T19180 476-479 Protein denotes p50
T19179 370-375 Protein denotes c-Rel
T19178 347-350 Protein denotes p52
T19177 89-93 Protein denotes NFAT

test3

Id Subject Object Predicate Lexical cue
T19194 643-649 Protein denotes NFATc1
T19193 615-621 Protein denotes NFATc2
T19192 589-594 Protein denotes c-rel
T19191 530-533 Protein denotes p52
T19190 504-507 Protein denotes p65
T19189 476-479 Protein denotes p50
T19188 370-375 Protein denotes c-Rel
T19187 347-350 Protein denotes p52
T19186 89-93 Protein denotes NFAT