> top > docs > PMC:1472690 > spans > 33414-34131 > annotations

PMC:1472690 / 33414-34131 JSONTXT

Annnotations TAB JSON ListView MergeView

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T17769 703-715 NN denotes BioWhittaker
T17768 702-703 -LRB- denotes (
T17767 690-701 NN denotes L-glutamine
T17766 687-689 NN denotes mM
T17765 683-686 CD denotes 200
T17764 681-682 NN denotes %
T17763 680-681 CD denotes 1
T17762 676-679 CC denotes and
T17761 674-675 -COMMA- denotes ,
T17760 673-674 -RRB- denotes )
T17759 668-673 NN denotes μg/ml
T17758 662-667 CD denotes 10000
T17757 661-662 -LRB- denotes (
T17756 648-660 NN denotes streptomycin
T17755 646-647 NN denotes %
T17754 645-646 CD denotes 1
T17753 643-644 -COMMA- denotes ,
T17752 642-643 -RRB- denotes )
T17751 638-642 NN denotes U/ml
T17750 632-637 CD denotes 10000
T17749 631-632 -LRB- denotes (
T17748 620-630 NN denotes penicillin
T17747 618-619 NN denotes %
T17746 617-618 CD denotes 1
T17745 615-616 -COMMA- denotes ,
T17744 614-615 -RRB- denotes )
T17743 607-614 NNP denotes Germany
T17742 605-606 -COMMA- denotes ,
T17741 596-605 NNP denotes Karlsruhe
T17740 594-595 -COMMA- denotes ,
T17739 584-594 NNP denotes Invitrogen
T17738 582-583 -COMMA- denotes ,
T17737 575-582 NN denotes low-LPS
T17736 573-574 -COMMA- denotes ,
T17735 570-573 NN denotes FCS
T17734 569-570 -LRB- denotes (
T17733 563-568 NN denotes serum
T17732 558-562 NN denotes calf
T17731 552-557 JJ denotes fetal
T17730 535-551 JJ denotes heat-inactivated
T17729 533-534 NN denotes %
T17728 531-533 CD denotes 10
T17727 520-530 VB denotes containing
T17726 518-519 -COMMA- denotes ,
T17725 517-518 -RRB- denotes )
T17724 509-517 JJ denotes LPS-free
T17723 507-508 -COMMA- denotes ,
T17722 500-507 NNP denotes Germany
T17721 498-499 -COMMA- denotes ,
T17720 488-498 NNP denotes Heidelberg
T17719 486-487 -COMMA- denotes ,
T17718 474-486 NNP denotes BioWhittaker
T17717 473-474 -LRB- denotes (
T17716 466-472 NN denotes medium
T17715 461-465 CD denotes 1640
T17714 456-460 NN denotes RPMI
T17713 453-455 IN denotes in
T17712 451-452 -RRB- denotes )
T17711 442-451 NN denotes cytometry
T17710 437-441 NN denotes flow
T17709 433-436 IN denotes for
T17708 432-433 -LRB- denotes (
T17707 423-431 NN denotes cells/ml
T17706 419-422 CD denotes 106
T17705 417-418 CC denotes ×
T17704 415-416 CD denotes 1
T17703 412-414 CC denotes or
T17702 410-411 -RRB- denotes )
T17701 404-410 NN denotes shifts
T17700 400-403 NN denotes gel
T17699 396-399 IN denotes for
T17698 395-396 -LRB- denotes (
T17697 388-394 NN denotes 106/ml
T17696 386-387 CC denotes ×
T17695 384-385 CD denotes 5
T17694 381-383 CC denotes or
T17693 379-380 -RRB- denotes )
T17692 367-379 NN denotes measurements
T17691 361-366 NN denotes ELISA
T17690 357-360 IN denotes for
T17689 356-357 -LRB- denotes (
T17688 347-355 NN denotes cells/ml
T17687 343-346 CD denotes 106
T17686 341-342 -COLON- denotes ×
T17685 339-340 CD denotes 3
T17684 336-338 IN denotes of
T17683 322-335 NN denotes concentration
T17682 316-321 JJ denotes final
T17681 314-315 DT denotes a
T17680 311-313 IN denotes at
T17679 302-310 VB denotes cultured
T17678 297-301 VB denotes were
T17677 292-296 NN denotes PBMC
T17676 290-291 -COMMA- denotes ,
T17675 281-290 NN denotes isolation
T17674 271-280 VB denotes Following
T17673 268-269 -RRB- denotes ]
T17672 263-268 CD denotes 54,55
T17671 262-263 -LRB- denotes [
T17670 253-261 NN denotes gradient
T17669 251-252 -RRB- denotes )
T17668 244-251 NNP denotes Germany
T17667 242-243 -COMMA- denotes ,
T17666 236-242 NNP denotes Berlin
T17665 234-235 -COMMA- denotes ,
T17664 226-234 NNP denotes Biochrom
T17663 225-226 -LRB- denotes (
T17662 210-224 JJ denotes Ficoll-Hypaque
T17661 208-209 DT denotes a
T17660 203-207 IN denotes over
T17659 193-202 VB denotes separated
T17658 188-192 VB denotes were
T17657 182-187 NN denotes coats
T17656 176-181 NN denotes buffy
T17655 172-175 DT denotes The
T17654 163-171 NNP denotes Luebeck.
T17653 160-162 IN denotes of
T17652 149-159 NNP denotes University
T17651 145-148 DT denotes the
T17650 142-144 IN denotes of
T17649 132-141 NN denotes committee
T17648 125-131 NN denotes ethics
T17647 121-124 DT denotes the
T17646 118-120 IN denotes of
T17645 109-117 NN denotes approval
T17644 105-108 DT denotes the
T17643 100-104 IN denotes with
T17642 96-99 CC denotes and
T17641 84-95 NN denotes declaration
T17640 75-83 NNP denotes Helsinki
T17639 71-74 DT denotes the
T17638 66-70 IN denotes with
T17637 55-65 NN denotes compliance
T17636 52-54 IN denotes in
T17635 45-51 NN denotes donors
T17634 39-44 NN denotes blood
T17633 31-38 JJ denotes healthy
T17632 26-30 IN denotes from
T17631 17-25 VB denotes obtained
T17630 12-16 VB denotes were
T17629 6-11 NN denotes coats
T17628 0-5 JJ denotes Buffy
T17770 715-716 -RRB- denotes )
R11916 T17629 T17628 arg1Of coats,Buffy
R11917 T17629 T17630 arg1Of coats,were
R11918 T17629 T17631 arg2Of coats,obtained
R11919 T17631 T17630 arg2Of obtained,were
R11920 T17631 T17632 arg1Of obtained,from
R11921 T17631 T17643 arg1Of obtained,with
R11922 T17632 T17642 arg1Of from,and
R11923 T17635 T17632 arg2Of donors,from
R11924 T17635 T17633 arg1Of donors,healthy
R11925 T17635 T17634 arg1Of donors,blood
R11926 T17635 T17636 arg1Of donors,in
R11927 T17637 T17636 arg2Of compliance,in
R11928 T17637 T17638 arg1Of compliance,with
R11929 T17641 T17638 arg2Of declaration,with
R11930 T17641 T17639 arg1Of declaration,the
R11931 T17641 T17640 arg1Of declaration,Helsinki
R11932 T17643 T17642 arg2Of with,and
R11933 T17645 T17643 arg2Of approval,with
R11934 T17645 T17644 arg1Of approval,the
R11935 T17645 T17646 arg1Of approval,of
R11936 T17649 T17646 arg2Of committee,of
R11937 T17649 T17647 arg1Of committee,the
R11938 T17649 T17648 arg1Of committee,ethics
R11939 T17649 T17650 arg1Of committee,of
R11940 T17649 T17658 modOf committee,were
R11941 T17652 T17650 arg2Of University,of
R11942 T17652 T17651 arg1Of University,the
R11943 T17652 T17653 arg1Of University,of
R11944 T17654 T17653 arg2Of Luebeck.,of
R11945 T17657 T17655 arg1Of coats,The
R11946 T17657 T17656 arg1Of coats,buffy
R11947 T17657 T17658 arg1Of coats,were
R11948 T17657 T17659 arg2Of coats,separated
R11949 T17659 T17658 arg2Of separated,were
R11950 T17659 T17660 arg1Of separated,over
R11951 T17659 T17671 arg1Of separated,[
R11952 T17662 T17663 arg1Of Ficoll-Hypaque,(
R11953 T17664 T17665 arg1Of Biochrom,","
R11954 T17665 T17663 arg2Of ",",(
R11955 T17665 T17667 arg1Of ",",","
R11956 T17666 T17665 arg2Of Berlin,","
R11957 T17668 T17667 arg2Of Germany,","
R11958 T17669 T17663 arg3Of ),(
R11959 T17670 T17660 arg2Of gradient,over
R11960 T17670 T17661 arg1Of gradient,a
R11961 T17670 T17662 arg1Of gradient,Ficoll-Hypaque
R11962 T17672 T17671 arg2Of "54,55",[
R11963 T17673 T17671 arg3Of ],[
R11964 T17675 T17674 arg2Of isolation,Following
R11965 T17677 T17678 arg1Of PBMC,were
R11966 T17677 T17679 arg2Of PBMC,cultured
R11967 T17679 T17674 arg1Of cultured,Following
R11968 T17679 T17676 arg1Of cultured,","
R11969 T17679 T17678 arg2Of cultured,were
R11970 T17679 T17680 arg1Of cultured,at
R11971 T17683 T17680 arg2Of concentration,at
R11972 T17683 T17681 arg1Of concentration,a
R11973 T17683 T17682 arg1Of concentration,final
R11974 T17683 T17684 arg1Of concentration,of
R11975 T17685 T17684 arg2Of 3,of
R11976 T17688 T17687 arg1Of cells/ml,106
R11977 T17688 T17689 arg1Of cells/ml,(
R11978 T17688 T17694 arg1Of cells/ml,or
R11979 T17690 T17689 arg2Of for,(
R11980 T17692 T17690 arg2Of measurements,for
R11981 T17692 T17691 arg1Of measurements,ELISA
R11982 T17693 T17689 arg3Of ),(
R11983 T17694 T17703 arg1Of or,or
R11984 T17695 T17696 arg1Of 5,×
R11985 T17696 T17694 arg2Of ×,or
R11986 T17696 T17698 arg1Of ×,(
R11987 T17697 T17696 arg2Of 106/ml,×
R11988 T17699 T17698 arg2Of for,(
R11989 T17701 T17699 arg2Of shifts,for
R11990 T17701 T17700 arg1Of shifts,gel
R11991 T17702 T17698 arg3Of ),(
R11992 T17703 T17680 arg3Of or,at
R11993 T17703 T17686 arg1Of or,×
R11994 T17704 T17705 arg1Of 1,×
R11995 T17706 T17705 arg2Of 106,×
R11996 T17707 T17704 arg1Of cells/ml,1
R11997 T17707 T17706 arg1Of cells/ml,106
R11998 T17707 T17708 arg1Of cells/ml,(
R11999 T17707 T17713 arg1Of cells/ml,in
R12000 T17707 T17726 arg1Of cells/ml,","
R12001 T17707 T17727 arg1Of cells/ml,containing
R12002 T17707 T17734 arg1Of cells/ml,(
R12003 T17707 T17745 arg1Of cells/ml,","
R12004 T17709 T17708 arg2Of for,(
R12005 T17711 T17709 arg2Of cytometry,for
R12006 T17711 T17710 arg1Of cytometry,flow
R12007 T17712 T17708 arg3Of ),(
R12008 T17716 T17713 arg2Of medium,in
R12009 T17716 T17714 arg1Of medium,RPMI
R12010 T17716 T17715 arg1Of medium,1640
R12011 T17716 T17717 arg1Of medium,(
R12012 T17718 T17719 arg1Of BioWhittaker,","
R12013 T17719 T17721 arg1Of ",",","
R12014 T17720 T17719 arg2Of Heidelberg,","
R12015 T17721 T17717 arg2Of ",",(
R12016 T17721 T17723 arg1Of ",",","
R12017 T17721 T17724 arg1Of ",",LPS-free
R12018 T17722 T17721 arg2Of Germany,","
R12019 T17725 T17717 arg3Of ),(
R12020 T17728 T17729 arg1Of 10,%
R12021 T17733 T17727 arg2Of serum,containing
R12022 T17733 T17728 arg1Of serum,10
R12023 T17733 T17730 arg1Of serum,heat-inactivated
R12024 T17733 T17731 arg1Of serum,fetal
R12025 T17733 T17732 arg1Of serum,calf
R12026 T17735 T17736 arg1Of FCS,","
R12027 T17735 T17738 arg1Of FCS,","
R12028 T17735 T17740 arg1Of FCS,","
R12029 T17737 T17736 arg2Of low-LPS,","
R12030 T17739 T17738 arg2Of Invitrogen,","
R12031 T17740 T17734 arg2Of ",",(
R12032 T17741 T17740 arg2Of Karlsruhe,","
R12033 T17741 T17742 arg1Of Karlsruhe,","
R12034 T17743 T17742 arg2Of Germany,","
R12035 T17744 T17734 arg3Of ),(
R12036 T17745 T17753 arg1Of ",",","
R12037 T17746 T17747 arg1Of 1,%
R12038 T17748 T17745 arg2Of penicillin,","
R12039 T17748 T17746 arg1Of penicillin,1
R12040 T17748 T17749 arg1Of penicillin,(
R12041 T17751 T17749 arg2Of U/ml,(
R12042 T17751 T17750 arg1Of U/ml,10000
R12043 T17752 T17749 arg3Of ),(
R12044 T17753 T17762 arg1Of ",",and
R12045 T17754 T17755 arg1Of 1,%
R12046 T17756 T17753 arg2Of streptomycin,","
R12047 T17756 T17754 arg1Of streptomycin,1
R12048 T17756 T17757 arg1Of streptomycin,(
R12049 T17759 T17757 arg2Of μg/ml,(
R12050 T17759 T17758 arg1Of μg/ml,10000
R12051 T17760 T17757 arg3Of ),(
R12052 T17762 T17703 arg2Of and,or
R12053 T17762 T17761 arg1Of and,","
R12054 T17765 T17766 arg1Of 200,mM
R12055 T17767 T17762 arg2Of L-glutamine,and
R12056 T17767 T17763 arg1Of L-glutamine,1
R12057 T17767 T17764 arg1Of L-glutamine,%
R12058 T17767 T17765 arg1Of L-glutamine,200
R12059 T17767 T17768 arg1Of L-glutamine,(
R12060 T17769 T17768 arg2Of BioWhittaker,(
R12061 T17770 T17768 arg3Of ),(

UBERON-AE

Id Subject Object Predicate Lexical cue
T17617 563-568 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T17616 39-44 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood

GO-CC

Id Subject Object Predicate Lexical cue
T17623 423-428 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17622 347-352 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T17620 271-717 Sentence denotes Following isolation, PBMC were cultured at a final concentration of 3 × 106 cells/ml (for ELISA measurements) or 5 × 106/ml (for gel shifts) or 1 × 106 cells/ml (for flow cytometry) in RPMI 1640 medium (BioWhittaker, Heidelberg, Germany, LPS-free), containing 10% heat-inactivated fetal calf serum (FCS, low-LPS, Invitrogen, Karlsruhe, Germany), 1% penicillin (10000 U/ml), 1% streptomycin (10000 μg/ml), and 1% 200 mM L-glutamine (BioWhittaker).
T17619 0-270 Sentence denotes Buffy coats were obtained from healthy blood donors in compliance with the Helsinki declaration and with the approval of the ethics committee of the University of Luebeck. The buffy coats were separated over a Ficoll-Hypaque (Biochrom, Berlin, Germany) gradient [54,55].
T232 0-171 Sentence denotes Buffy coats were obtained from healthy blood donors in compliance with the Helsinki declaration and with the approval of the ethics committee of the University of Luebeck.
T233 172-270 Sentence denotes The buffy coats were separated over a Ficoll-Hypaque (Biochrom, Berlin, Germany) gradient [54,55].
T234 271-717 Sentence denotes Following isolation, PBMC were cultured at a final concentration of 3 × 106 cells/ml (for ELISA measurements) or 5 × 106/ml (for gel shifts) or 1 × 106 cells/ml (for flow cytometry) in RPMI 1640 medium (BioWhittaker, Heidelberg, Germany, LPS-free), containing 10% heat-inactivated fetal calf serum (FCS, low-LPS, Invitrogen, Karlsruhe, Germany), 1% penicillin (10000 U/ml), 1% streptomycin (10000 μg/ml), and 1% 200 mM L-glutamine (BioWhittaker).

ICD10

Id Subject Object Predicate Lexical cue
T17621 39-51 http://purl.bioontology.org/ontology/ICD10/Z52.0 denotes blood donors

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T17930 617-630 Entity denotes 1% penicillin
T17929 680-716 Entity denotes 1% 200 mM L-glutamine (BioWhittaker)
T17927 292-296 Entity denotes PBMC
T17926 400-403 Entity denotes gel
T17925 645-675 Entity denotes 1% streptomycin (10000 μg/ml),
T17924 575-582 Entity denotes low-LPS
T17923 509-517 Entity denotes LPS-free

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T17922 716-717 . denotes .
T17921 715-716 -RRB- denotes )
T17920 703-715 NNP denotes BioWhittaker
T17919 702-703 -LRB- denotes (
T17918 690-701 NNP denotes L-glutamine
T17917 687-689 NNP denotes mM
T17916 683-686 CD denotes 200
T17915 681-682 NN denotes %
T17914 680-681 CD denotes 1
T17913 676-679 CC denotes and
T17912 674-675 , denotes ,
T17911 673-674 -RRB- denotes )
T17910 671-673 NN denotes ml
T17909 670-671 NN denotes /
T17908 668-670 NN denotes μg
T17907 662-667 CD denotes 10000
T17906 661-662 -LRB- denotes (
T17905 648-660 NN denotes streptomycin
T17904 646-647 NN denotes %
T17903 645-646 CD denotes 1
T17902 643-644 , denotes ,
T17901 642-643 -RRB- denotes )
T17900 638-642 NNP denotes U/ml
T17899 632-637 CD denotes 10000
T17898 631-632 -LRB- denotes (
T17897 620-630 NN denotes penicillin
T17896 618-619 NN denotes %
T17895 617-618 CD denotes 1
T17894 615-616 , denotes ,
T17893 614-615 -RRB- denotes )
T17892 607-614 NNP denotes Germany
T17891 605-606 , denotes ,
T17890 596-605 NNP denotes Karlsruhe
T17889 594-595 , denotes ,
T17888 584-594 NNP denotes Invitrogen
T17887 582-583 , denotes ,
T17886 575-582 NNP denotes low-LPS
T17885 573-574 , denotes ,
T17884 570-573 NNP denotes FCS
T17883 569-570 -LRB- denotes (
T17882 563-568 NN denotes serum
T17881 558-562 NN denotes calf
T17880 552-557 JJ denotes fetal
T17879 535-551 JJ denotes heat-inactivated
T17878 533-534 NN denotes %
T17877 531-533 CD denotes 10
T17876 520-530 VBG denotes containing
T17875 518-519 , denotes ,
T17874 517-518 -RRB- denotes )
T17873 509-517 JJ denotes LPS-free
T17872 507-508 , denotes ,
T17871 500-507 NNP denotes Germany
T17870 498-499 , denotes ,
T17869 488-498 NNP denotes Heidelberg
T17868 486-487 , denotes ,
T17867 474-486 NNP denotes BioWhittaker
T17866 473-474 -LRB- denotes (
T17865 466-472 NN denotes medium
T17864 461-465 CD denotes 1640
T17863 456-460 NNP denotes RPMI
T17862 453-455 IN denotes in
T17861 451-452 -RRB- denotes )
T17860 442-451 NN denotes cytometry
T17859 437-441 NN denotes flow
T17858 433-436 IN denotes for
T17857 432-433 -LRB- denotes (
T17856 423-431 NN denotes cells/ml
T17855 419-422 CD denotes 106
T17854 417-418 CD denotes ×
T17853 415-416 CD denotes 1
T17852 412-414 CC denotes or
T17851 410-411 -RRB- denotes )
T17850 404-410 NNS denotes shifts
T17849 400-403 NN denotes gel
T17848 396-399 IN denotes for
T17847 395-396 -LRB- denotes (
T17846 388-394 CD denotes 106/ml
T17845 386-387 NN denotes ×
T17844 384-385 CD denotes 5
T17843 381-383 CC denotes or
T17842 379-380 -RRB- denotes )
T17841 367-379 NNS denotes measurements
T17840 361-366 NNP denotes ELISA
T17839 357-360 IN denotes for
T17838 356-357 -LRB- denotes (
T17837 347-355 NN denotes cells/ml
T17836 343-346 CD denotes 106
T17835 341-342 CD denotes ×
T17834 339-340 CD denotes 3
T17833 336-338 IN denotes of
T17832 322-335 NN denotes concentration
T17831 316-321 JJ denotes final
T17830 314-315 DT denotes a
T17829 311-313 IN denotes at
T17828 302-310 VBN denotes cultured
T17827 297-301 VBD denotes were
T17826 292-296 NNP denotes PBMC
T17825 290-291 , denotes ,
T17824 281-290 NN denotes isolation
T17823 271-280 VBG denotes Following
T17822 269-270 . denotes .
T17821 268-269 NNP denotes ]
T17820 263-268 CD denotes 54,55
T17819 262-263 NNP denotes [
T17818 253-261 NN denotes gradient
T17817 251-252 -RRB- denotes )
T17816 244-251 NNP denotes Germany
T17815 242-243 , denotes ,
T17814 236-242 NNP denotes Berlin
T17813 234-235 , denotes ,
T17812 226-234 NNP denotes Biochrom
T17811 225-226 -LRB- denotes (
T17810 210-224 NNP denotes Ficoll-Hypaque
T17809 208-209 DT denotes a
T17808 203-207 IN denotes over
T17807 193-202 VBN denotes separated
T17806 188-192 VBD denotes were
T17805 182-187 NNS denotes coats
T17804 176-181 JJ denotes buffy
T17803 172-175 DT denotes The
T17802 170-171 . denotes .
T17801 163-170 NNP denotes Luebeck
T17800 160-162 IN denotes of
T17799 149-159 NNP denotes University
T17798 145-148 DT denotes the
T17797 142-144 IN denotes of
T17796 132-141 NN denotes committee
T17795 125-131 NNS denotes ethics
T17794 121-124 DT denotes the
T17793 118-120 IN denotes of
T17792 109-117 NN denotes approval
T17791 105-108 DT denotes the
T17790 100-104 IN denotes with
T17789 96-99 CC denotes and
T17788 84-95 NN denotes declaration
T17787 75-83 NNP denotes Helsinki
T17786 71-74 DT denotes the
T17785 66-70 IN denotes with
T17784 55-65 NN denotes compliance
T17783 52-54 IN denotes in
T17782 45-51 NNS denotes donors
T17781 39-44 NN denotes blood
T17780 31-38 JJ denotes healthy
T17779 26-30 IN denotes from
T17778 17-25 VBN denotes obtained
T17777 12-16 VBD denotes were
T17776 6-11 NNS denotes coats
T17775 0-5 NNP denotes Buffy
R12086 T17795 T17796 compound ethics,committee
R12087 T17796 T17793 pobj committee,of
R12088 T17797 T17796 prep of,committee
R12089 T17798 T17799 det the,University
R12090 T17799 T17797 pobj University,of
R12091 T17800 T17799 prep of,University
R12092 T17801 T17800 pobj Luebeck,of
R12093 T17802 T17778 punct .,obtained
R12094 T17803 T17805 det The,coats
R12095 T17804 T17805 amod buffy,coats
R12096 T17805 T17807 nsubjpass coats,separated
R12097 T17806 T17807 auxpass were,separated
R12098 T17807 T17807 ROOT separated,separated
R12099 T17808 T17807 prep over,separated
R12100 T17809 T17810 det a,Ficoll-Hypaque
R12101 T17810 T17808 pobj Ficoll-Hypaque,over
R12102 T17811 T17818 punct (,gradient
R12103 T17812 T17818 nmod Biochrom,gradient
R12104 T17813 T17812 punct ",",Biochrom
R12105 T17814 T17812 conj Berlin,Biochrom
R12106 T17815 T17814 punct ",",Berlin
R12107 T17816 T17814 conj Germany,Berlin
R12108 T17817 T17818 punct ),gradient
R12145 T17854 T17856 nummod ×,cells/ml
R12146 T17855 T17856 nummod 106,cells/ml
R12147 T17856 T17845 conj cells/ml,×
R12148 T17857 T17858 punct (,for
R12149 T17858 T17856 prep for,cells/ml
R12150 T17859 T17860 compound flow,cytometry
R12151 T17860 T17858 pobj cytometry,for
R12182 T17891 T17890 punct ",",Karlsruhe
R12183 T17892 T17890 conj Germany,Karlsruhe
R12184 T17893 T17890 punct ),Karlsruhe
R12185 T17894 T17888 punct ",",Invitrogen
R12186 T17895 T17896 nummod 1,%
R12187 T17896 T17897 compound %,penicillin
R12188 T17897 T17888 appos penicillin,Invitrogen
R12189 T17898 T17900 punct (,U/ml
R12190 T17899 T17900 nummod 10000,U/ml
R12191 T17900 T17897 appos U/ml,penicillin
R12192 T17901 T17900 punct ),U/ml
R12193 T17902 T17897 punct ",",penicillin
R12194 T17903 T17904 nummod 1,%
R12195 T17904 T17905 compound %,streptomycin
R12196 T17905 T17882 appos streptomycin,serum
R12197 T17906 T17905 punct (,streptomycin
R12198 T17907 T17910 nummod 10000,ml
R12199 T17908 T17910 compound μg,ml
R12200 T17909 T17910 compound /,ml
R12201 T17910 T17905 appos ml,streptomycin
R12202 T17911 T17905 punct ),streptomycin
R12203 T17912 T17905 punct ",",streptomycin
R12204 T17913 T17882 cc and,serum
R12205 T17914 T17915 nummod 1,%
R12206 T17915 T17876 npadvmod %,containing
R12207 T17916 T17918 nummod 200,L-glutamine
R12208 T17917 T17918 compound mM,L-glutamine
R12209 T17918 T17918 ROOT L-glutamine,L-glutamine
R12210 T17919 T17920 punct (,BioWhittaker
R12211 T17920 T17918 appos BioWhittaker,L-glutamine
R12212 T17921 T17918 punct ),L-glutamine
R12213 T17922 T17828 punct .,cultured
R12066 T17775 T17776 compound Buffy,coats
R12068 T17777 T17778 auxpass were,obtained
R12069 T17778 T17778 ROOT obtained,obtained
R12070 T17779 T17778 prep from,obtained
R12071 T17780 T17782 amod healthy,donors
R12072 T17781 T17782 compound blood,donors
R12073 T17782 T17779 pobj donors,from
R12074 T17783 T17778 prep in,obtained
R12075 T17784 T17783 pobj compliance,in
R12076 T17785 T17784 prep with,compliance
R12077 T17786 T17788 det the,declaration
R12078 T17787 T17788 compound Helsinki,declaration
R12079 T17788 T17785 pobj declaration,with
R12080 T17789 T17785 cc and,with
R12081 T17790 T17785 conj with,with
R12082 T17791 T17792 det the,approval
R12083 T17792 T17790 pobj approval,with
R12084 T17793 T17792 prep of,approval
R12085 T17794 T17796 det the,committee
R12109 T17818 T17821 compound gradient,]
R12110 T17819 T17821 compound [,]
R12111 T17820 T17821 compound "54,55",]
R12112 T17821 T17807 npadvmod ],separated
R12113 T17822 T17807 punct .,separated
R12114 T17823 T17828 prep Following,cultured
R12115 T17824 T17823 pobj isolation,Following
R12116 T17825 T17828 punct ",",cultured
R12117 T17826 T17828 nsubjpass PBMC,cultured
R12118 T17827 T17828 auxpass were,cultured
R12119 T17828 T17828 ROOT cultured,cultured
R12120 T17829 T17828 prep at,cultured
R12121 T17830 T17832 det a,concentration
R12122 T17831 T17832 amod final,concentration
R12123 T17832 T17829 pobj concentration,at
R12124 T17833 T17832 prep of,concentration
R12125 T17834 T17837 nummod 3,cells/ml
R12126 T17835 T17837 nummod ×,cells/ml
R12127 T17836 T17837 nummod 106,cells/ml
R12128 T17837 T17833 pobj cells/ml,of
R12129 T17838 T17837 punct (,cells/ml
R12130 T17839 T17837 prep for,cells/ml
R12131 T17840 T17841 compound ELISA,measurements
R12132 T17841 T17839 pobj measurements,for
R12133 T17842 T17837 punct ),cells/ml
R12134 T17843 T17832 cc or,concentration
R12135 T17844 T17845 nummod 5,×
R12136 T17845 T17876 nmod ×,containing
R12137 T17846 T17845 nummod 106/ml,×
R12138 T17847 T17848 punct (,for
R12139 T17848 T17845 prep for,×
R12140 T17849 T17850 compound gel,shifts
R12141 T17850 T17848 pobj shifts,for
R12142 T17851 T17848 punct ),for
R12143 T17852 T17845 cc or,×
R12144 T17853 T17856 nummod 1,cells/ml
R12152 T17861 T17858 punct ),for
R12153 T17862 T17856 prep in,cells/ml
R12154 T17863 T17865 nmod RPMI,medium
R12155 T17864 T17865 nummod 1640,medium
R12156 T17865 T17862 pobj medium,in
R12157 T17866 T17876 punct (,containing
R12158 T17867 T17876 dep BioWhittaker,containing
R12159 T17868 T17867 punct ",",BioWhittaker
R12160 T17869 T17867 conj Heidelberg,BioWhittaker
R12161 T17870 T17869 punct ",",Heidelberg
R12162 T17871 T17867 npadvmod Germany,BioWhittaker
R12163 T17872 T17867 punct ",",BioWhittaker
R12164 T17873 T17867 appos LPS-free,BioWhittaker
R12165 T17874 T17873 punct ),LPS-free
R12166 T17875 T17876 punct ",",containing
R12167 T17876 T17828 advcl containing,cultured
R12168 T17877 T17878 nummod 10,%
R12169 T17878 T17882 nmod %,serum
R12170 T17879 T17882 amod heat-inactivated,serum
R12171 T17880 T17881 amod fetal,calf
R12172 T17881 T17882 compound calf,serum
R12173 T17882 T17876 dobj serum,containing
R12174 T17883 T17905 punct (,streptomycin
R12175 T17884 T17905 nmod FCS,streptomycin
R12176 T17885 T17884 punct ",",FCS
R12177 T17886 T17884 conj low-LPS,FCS
R12178 T17887 T17886 punct ",",low-LPS
R12179 T17888 T17886 conj Invitrogen,low-LPS
R12180 T17889 T17888 punct ",",Invitrogen
R12181 T17890 T17888 conj Karlsruhe,Invitrogen