Id |
Subject |
Object |
Predicate |
Lexical cue |
T5031 |
0-1 |
DT |
denotes |
A |
T5032 |
14-22 |
NN |
denotes |
subclone |
T5033 |
2-9 |
JJ |
denotes |
genomic |
T5034 |
10-13 |
NN |
denotes |
DNA |
T5035 |
123-131 |
VBN |
denotes |
obtained |
T5036 |
23-25 |
IN |
denotes |
of |
T5037 |
26-32 |
NN |
denotes |
Strain |
T5038 |
41-44 |
NN |
denotes |
DNA |
T5039 |
33-36 |
CD |
denotes |
129 |
T5040 |
37-40 |
NN |
denotes |
SvJ |
T5041 |
36-37 |
HYPH |
denotes |
/ |
T5042 |
45-49 |
WDT |
denotes |
that |
T5043 |
50-59 |
VBD |
denotes |
contained |
T5044 |
60-61 |
DT |
denotes |
a |
T5045 |
62-69 |
NN |
denotes |
portion |
T5046 |
70-72 |
IN |
denotes |
of |
T5047 |
73-78 |
NNS |
denotes |
Exons |
T5048 |
79-80 |
CD |
denotes |
4 |
T5049 |
81-84 |
CC |
denotes |
and |
T5050 |
85-86 |
CD |
denotes |
5 |
T5051 |
87-89 |
IN |
denotes |
of |
T5052 |
90-93 |
DT |
denotes |
the |
T5053 |
97-101 |
NN |
denotes |
gene |
T5054 |
94-96 |
NN |
denotes |
E2 |
T5055 |
102-105 |
CC |
denotes |
and |
T5056 |
106-114 |
NN |
denotes |
flanking |
T5057 |
115-118 |
NN |
denotes |
DNA |
T5058 |
119-122 |
VBD |
denotes |
was |
T5059 |
132-136 |
IN |
denotes |
from |
T5060 |
137-138 |
DT |
denotes |
a |
T5061 |
148-155 |
NN |
denotes |
library |
T5062 |
139-141 |
NN |
denotes |
P1 |
T5063 |
142-147 |
NN |
denotes |
phage |
T5064 |
156-160 |
IN |
denotes |
from |
T5065 |
161-167 |
NNP |
denotes |
Genome |
T5066 |
177-181 |
NNP |
denotes |
Inc. |
T5067 |
168-175 |
NNP |
denotes |
Systems |
T5068 |
175-177 |
, |
denotes |
, |
T5069 |
181-183 |
, |
denotes |
, |
T5070 |
183-184 |
-LRB- |
denotes |
( |
T5071 |
215-222 |
NNP |
denotes |
Library |
T5072 |
184-187 |
NNP |
denotes |
St. |
T5073 |
188-193 |
NNP |
denotes |
Louis |
T5074 |
193-195 |
, |
denotes |
, |
T5075 |
195-197 |
NNP |
denotes |
MO |
T5076 |
197-198 |
: |
denotes |
; |
T5077 |
199-200 |
SYM |
denotes |
' |
T5078 |
200-201 |
CD |
denotes |
3 |
T5079 |
202-205 |
NN |
denotes |
Hit |
T5080 |
201-202 |
HYPH |
denotes |
- |
T5081 |
206-211 |
NNP |
denotes |
Mouse |
T5082 |
212-214 |
NNP |
denotes |
ES |
T5083 |
222-223 |
POS |
denotes |
' |
T5084 |
223-224 |
-RRB- |
denotes |
) |
T5085 |
224-225 |
. |
denotes |
. |
T5086 |
225-465 |
sentence |
denotes |
A positive/negative replacement type gene targeting vector [21] was created by replacing a 1.67 kb EcoRV-Smal fragment that included a portion of Exon 4 and all of Exon 5 with the PGKneo marker gene (See Fig. 1A) from the pPNT vector [22]. |
T5087 |
226-227 |
DT |
denotes |
A |
T5088 |
278-284 |
NN |
denotes |
vector |
T5089 |
228-236 |
JJ |
denotes |
positive |
T5090 |
237-245 |
JJ |
denotes |
negative |
T5091 |
236-237 |
HYPH |
denotes |
/ |
T5092 |
246-257 |
NN |
denotes |
replacement |
T5093 |
258-262 |
NN |
denotes |
type |
T5094 |
263-267 |
NN |
denotes |
gene |
T5095 |
268-277 |
NN |
denotes |
targeting |
T5096 |
294-301 |
VBN |
denotes |
created |
T5097 |
285-286 |
-LRB- |
denotes |
[ |
T5098 |
286-288 |
CD |
denotes |
21 |
T5099 |
288-289 |
-RRB- |
denotes |
] |
T5100 |
290-293 |
VBD |
denotes |
was |
T5101 |
302-304 |
IN |
denotes |
by |
T5102 |
305-314 |
VBG |
denotes |
replacing |
T5103 |
315-316 |
DT |
denotes |
a |
T5104 |
336-344 |
NN |
denotes |
fragment |
T5105 |
317-321 |
CD |
denotes |
1.67 |
T5106 |
322-324 |
NN |
denotes |
kb |
T5107 |
325-330 |
NN |
denotes |
EcoRV |
T5108 |
331-335 |
NN |
denotes |
Smal |
T5109 |
330-331 |
HYPH |
denotes |
- |
T5110 |
345-349 |
WDT |
denotes |
that |
T5111 |
350-358 |
VBD |
denotes |
included |
T5112 |
359-360 |
DT |
denotes |
a |
T5113 |
361-368 |
NN |
denotes |
portion |
T5114 |
369-371 |
IN |
denotes |
of |
T5115 |
372-376 |
NN |
denotes |
Exon |
T5116 |
377-378 |
CD |
denotes |
4 |
T5117 |
379-382 |
CC |
denotes |
and |
T5118 |
383-386 |
DT |
denotes |
all |
T5119 |
387-389 |
IN |
denotes |
of |
T5120 |
390-394 |
NN |
denotes |
Exon |
T5121 |
395-396 |
CD |
denotes |
5 |
T5122 |
397-401 |
IN |
denotes |
with |
T5123 |
402-405 |
DT |
denotes |
the |
T5124 |
420-424 |
NN |
denotes |
gene |
T5125 |
406-412 |
NN |
denotes |
PGKneo |
T5126 |
413-419 |
NN |
denotes |
marker |
T5127 |
425-426 |
-LRB- |
denotes |
( |
T5128 |
426-429 |
VB |
denotes |
See |
T5129 |
430-434 |
NN |
denotes |
Fig. |
T5130 |
435-437 |
NN |
denotes |
1A |
T5131 |
437-438 |
-RRB- |
denotes |
) |
T5132 |
439-443 |
IN |
denotes |
from |
T5133 |
444-447 |
DT |
denotes |
the |
T5134 |
453-459 |
NN |
denotes |
vector |
T5135 |
448-452 |
NN |
denotes |
pPNT |
T5136 |
460-461 |
-LRB- |
denotes |
[ |
T5137 |
461-463 |
CD |
denotes |
22 |
T5138 |
463-464 |
-RRB- |
denotes |
] |
T5139 |
464-465 |
. |
denotes |
. |
T5140 |
465-599 |
sentence |
denotes |
The targeting construct was linearized with NotI and electroporated into R1 ES cells [23] under conditions described previously [24]. |
T5141 |
466-469 |
DT |
denotes |
The |
T5142 |
480-489 |
NN |
denotes |
construct |
T5143 |
470-479 |
NN |
denotes |
targeting |
T5144 |
494-504 |
VBN |
denotes |
linearized |
T5145 |
490-493 |
VBD |
denotes |
was |
T5146 |
505-509 |
IN |
denotes |
with |
T5147 |
510-514 |
NN |
denotes |
NotI |
T5148 |
515-518 |
CC |
denotes |
and |
T5149 |
519-533 |
VBN |
denotes |
electroporated |
T5150 |
534-538 |
IN |
denotes |
into |
T5151 |
539-541 |
NN |
denotes |
R1 |
T5152 |
545-550 |
NNS |
denotes |
cells |
T5153 |
542-544 |
NN |
denotes |
ES |
T5154 |
551-552 |
-LRB- |
denotes |
[ |
T5155 |
552-554 |
CD |
denotes |
23 |
T5156 |
554-555 |
-RRB- |
denotes |
] |
T5157 |
556-561 |
IN |
denotes |
under |
T5158 |
562-572 |
NNS |
denotes |
conditions |
T5159 |
573-582 |
VBN |
denotes |
described |
T5160 |
583-593 |
RB |
denotes |
previously |
T5161 |
594-595 |
-LRB- |
denotes |
[ |
T5162 |
595-597 |
CD |
denotes |
24 |
T5163 |
597-598 |
-RRB- |
denotes |
] |
T5164 |
598-599 |
. |
denotes |
. |
T5165 |
599-741 |
sentence |
denotes |
ES cells were selected with G418 (300 μg/ml; Life Technologies Inc., Gaithersburg, MD) and gancyclovir (2 μM; gift of Syntex, Palo Alto, CA). |
T5166 |
600-602 |
NN |
denotes |
ES |
T5167 |
603-608 |
NNS |
denotes |
cells |
T5168 |
614-622 |
VBN |
denotes |
selected |
T5169 |
609-613 |
VBD |
denotes |
were |
T5170 |
623-627 |
IN |
denotes |
with |
T5171 |
628-632 |
NN |
denotes |
G418 |
T5172 |
633-634 |
-LRB- |
denotes |
( |
T5173 |
663-667 |
NNP |
denotes |
Inc. |
T5174 |
634-637 |
CD |
denotes |
300 |
T5175 |
638-640 |
NN |
denotes |
μg |
T5176 |
640-641 |
SYM |
denotes |
/ |
T5177 |
641-643 |
NN |
denotes |
ml |
T5178 |
643-644 |
: |
denotes |
; |
T5179 |
645-649 |
NNP |
denotes |
Life |
T5180 |
650-662 |
NNP |
denotes |
Technologies |
T5181 |
667-669 |
, |
denotes |
, |
T5182 |
669-681 |
NNP |
denotes |
Gaithersburg |
T5183 |
681-683 |
, |
denotes |
, |
T5184 |
683-685 |
NNP |
denotes |
MD |
T5185 |
685-686 |
-RRB- |
denotes |
) |
T5186 |
687-690 |
CC |
denotes |
and |
T5187 |
691-702 |
NN |
denotes |
gancyclovir |
T5188 |
703-704 |
-LRB- |
denotes |
( |
T5189 |
704-705 |
CD |
denotes |
2 |
T5190 |
706-708 |
NN |
denotes |
μM |
T5191 |
708-709 |
: |
denotes |
; |
T5192 |
710-714 |
NN |
denotes |
gift |
T5193 |
715-717 |
IN |
denotes |
of |
T5194 |
718-724 |
NNP |
denotes |
Syntex |
T5195 |
724-726 |
, |
denotes |
, |
T5196 |
726-730 |
NNP |
denotes |
Palo |
T5197 |
731-735 |
NNP |
denotes |
Alto |
T5198 |
735-737 |
, |
denotes |
, |
T5199 |
737-739 |
NNP |
denotes |
CA |
T5200 |
739-740 |
-RRB- |
denotes |
) |
T5201 |
740-741 |
. |
denotes |
. |
T5202 |
741-854 |
sentence |
denotes |
Doubly resistant clones were screened for gene targeting by Southern blot analysis of BglI digested genomic DNA. |
T5203 |
742-748 |
RB |
denotes |
Doubly |
T5204 |
749-758 |
JJ |
denotes |
resistant |
T5205 |
759-765 |
NNS |
denotes |
clones |
T5206 |
771-779 |
VBN |
denotes |
screened |
T5207 |
766-770 |
VBD |
denotes |
were |
T5208 |
780-783 |
IN |
denotes |
for |
T5209 |
784-788 |
NN |
denotes |
gene |
T5210 |
789-798 |
NN |
denotes |
targeting |
T5211 |
799-801 |
IN |
denotes |
by |
T5212 |
802-810 |
NNP |
denotes |
Southern |
T5213 |
811-815 |
NN |
denotes |
blot |
T5214 |
816-824 |
NN |
denotes |
analysis |
T5215 |
825-827 |
IN |
denotes |
of |
T5216 |
828-832 |
NN |
denotes |
BglI |
T5217 |
850-853 |
NN |
denotes |
DNA |
T5218 |
833-841 |
VBN |
denotes |
digested |
T5219 |
842-849 |
JJ |
denotes |
genomic |
T5220 |
853-854 |
. |
denotes |
. |
T5221 |
854-957 |
sentence |
denotes |
Blots were hybridized with an Exon 6 specific probe that was external to the gene targeting construct. |
T5222 |
855-860 |
NNS |
denotes |
Blots |
T5223 |
866-876 |
VBN |
denotes |
hybridized |
T5224 |
861-865 |
VBD |
denotes |
were |
T5225 |
877-881 |
IN |
denotes |
with |
T5226 |
882-884 |
DT |
denotes |
an |
T5227 |
901-906 |
NN |
denotes |
probe |
T5228 |
885-889 |
NN |
denotes |
Exon |
T5229 |
892-900 |
JJ |
denotes |
specific |
T5230 |
890-891 |
CD |
denotes |
6 |
T5231 |
907-911 |
WDT |
denotes |
that |
T5232 |
912-915 |
VBD |
denotes |
was |
T5233 |
916-924 |
JJ |
denotes |
external |
T5234 |
925-927 |
IN |
denotes |
to |
T5235 |
928-931 |
DT |
denotes |
the |
T5236 |
947-956 |
NN |
denotes |
construct |
T5237 |
932-936 |
NN |
denotes |
gene |
T5238 |
937-946 |
NN |
denotes |
targeting |
T5239 |
956-957 |
. |
denotes |
. |
T5240 |
957-1077 |
sentence |
denotes |
Correctly targeted ES cells were injected into C57BL/6J blastocysts to produce chimeric mice using standard procedures. |
T5241 |
958-967 |
RB |
denotes |
Correctly |
T5242 |
968-976 |
VBN |
denotes |
targeted |
T5243 |
980-985 |
NNS |
denotes |
cells |
T5244 |
977-979 |
NN |
denotes |
ES |
T5245 |
991-999 |
VBN |
denotes |
injected |
T5246 |
986-990 |
VBD |
denotes |
were |
T5247 |
1000-1004 |
IN |
denotes |
into |
T5248 |
1005-1010 |
NN |
denotes |
C57BL |
T5249 |
1011-1013 |
NN |
denotes |
6J |
T5250 |
1010-1011 |
HYPH |
denotes |
/ |
T5251 |
1014-1025 |
NNS |
denotes |
blastocysts |
T5252 |
1026-1028 |
TO |
denotes |
to |
T5253 |
1029-1036 |
VB |
denotes |
produce |
T5254 |
1037-1045 |
JJ |
denotes |
chimeric |
T5255 |
1046-1050 |
NNS |
denotes |
mice |
T5256 |
1051-1056 |
VBG |
denotes |
using |
T5257 |
1057-1065 |
JJ |
denotes |
standard |
T5258 |
1066-1076 |
NNS |
denotes |
procedures |
T5259 |
1076-1077 |
. |
denotes |
. |
T5260 |
1077-1234 |
sentence |
denotes |
Heterozygous offspring from germline competent chimeras were intercrossed to produce wild type (+/+), heterozygous (+/-) and homozygous knockout (-/-) mice. |
T5261 |
1078-1090 |
JJ |
denotes |
Heterozygous |
T5262 |
1091-1100 |
NN |
denotes |
offspring |
T5263 |
1139-1151 |
VBN |
denotes |
intercrossed |
T5264 |
1101-1105 |
IN |
denotes |
from |
T5265 |
1106-1114 |
NN |
denotes |
germline |
T5266 |
1115-1124 |
JJ |
denotes |
competent |
T5267 |
1125-1133 |
NNS |
denotes |
chimeras |
T5268 |
1134-1138 |
VBD |
denotes |
were |
T5269 |
1152-1154 |
TO |
denotes |
to |
T5270 |
1155-1162 |
VB |
denotes |
produce |
T5271 |
1163-1167 |
JJ |
denotes |
wild |
T5272 |
1168-1172 |
NN |
denotes |
type |
T5273 |
1214-1222 |
NN |
denotes |
knockout |
T5274 |
1173-1174 |
-LRB- |
denotes |
( |
T5275 |
1176-1177 |
SYM |
denotes |
+ |
T5276 |
1174-1175 |
SYM |
denotes |
+ |
T5277 |
1175-1176 |
HYPH |
denotes |
/ |
T5278 |
1177-1178 |
-RRB- |
denotes |
) |
T5279 |
1178-1180 |
, |
denotes |
, |
T5280 |
1180-1192 |
JJ |
denotes |
heterozygous |
T5281 |
1193-1194 |
-LRB- |
denotes |
( |
T5282 |
1196-1197 |
SYM |
denotes |
- |
T5283 |
1194-1195 |
SYM |
denotes |
+ |
T5284 |
1195-1196 |
HYPH |
denotes |
/ |
T5285 |
1197-1198 |
-RRB- |
denotes |
) |
T5286 |
1199-1202 |
CC |
denotes |
and |
T5287 |
1203-1213 |
JJ |
denotes |
homozygous |
T5288 |
1229-1233 |
NNS |
denotes |
mice |
T5289 |
1223-1224 |
-LRB- |
denotes |
( |
T5290 |
1226-1227 |
SYM |
denotes |
- |
T5291 |
1224-1225 |
SYM |
denotes |
- |
T5292 |
1225-1226 |
HYPH |
denotes |
/ |
T5293 |
1227-1228 |
-RRB- |
denotes |
) |
T5294 |
1233-1234 |
. |
denotes |
. |
T5295 |
1234-1284 |
sentence |
denotes |
At all generations, +/- breeding pairs were used. |
T5296 |
1235-1237 |
IN |
denotes |
At |
T5297 |
1279-1283 |
VBN |
denotes |
used |
T5298 |
1238-1241 |
DT |
denotes |
all |
T5299 |
1242-1253 |
NNS |
denotes |
generations |
T5300 |
1253-1255 |
, |
denotes |
, |
T5301 |
1255-1256 |
SYM |
denotes |
+ |
T5302 |
1257-1258 |
SYM |
denotes |
- |
T5303 |
1256-1257 |
HYPH |
denotes |
/ |
T5304 |
1268-1273 |
NNS |
denotes |
pairs |
T5305 |
1259-1267 |
VBG |
denotes |
breeding |
T5306 |
1274-1278 |
VBD |
denotes |
were |
T5307 |
1283-1284 |
. |
denotes |
. |
T5308 |
1284-1355 |
sentence |
denotes |
Results presented here are from mice derived from the F2+ generations. |
T5309 |
1285-1292 |
NNS |
denotes |
Results |
T5310 |
1308-1311 |
VBP |
denotes |
are |
T5311 |
1293-1302 |
VBN |
denotes |
presented |
T5312 |
1303-1307 |
RB |
denotes |
here |
T5313 |
1312-1316 |
IN |
denotes |
from |
T5314 |
1317-1321 |
NNS |
denotes |
mice |
T5315 |
1322-1329 |
VBN |
denotes |
derived |
T5316 |
1330-1334 |
IN |
denotes |
from |
T5317 |
1335-1338 |
DT |
denotes |
the |
T5318 |
1343-1354 |
NNS |
denotes |
generations |
T5319 |
1339-1341 |
NN |
denotes |
F2 |
T5320 |
1341-1342 |
SYM |
denotes |
+ |
T5321 |
1354-1355 |
. |
denotes |
. |
T5322 |
1355-1431 |
sentence |
denotes |
All animals were of a mixed C57BL/6J × Strain 129Sv/SvJ genetic background. |
T5323 |
1356-1359 |
DT |
denotes |
All |
T5324 |
1360-1367 |
NNS |
denotes |
animals |
T5325 |
1368-1372 |
VBD |
denotes |
were |
T5326 |
1373-1375 |
IN |
denotes |
of |
T5327 |
1376-1377 |
DT |
denotes |
a |
T5328 |
1420-1430 |
NN |
denotes |
background |
T5329 |
1378-1383 |
VBN |
denotes |
mixed |
T5330 |
1384-1389 |
NN |
denotes |
C57BL |
T5331 |
1390-1392 |
NN |
denotes |
6J |
T5332 |
1389-1390 |
HYPH |
denotes |
/ |
T5333 |
1393-1394 |
SYM |
denotes |
× |
T5334 |
1395-1401 |
NN |
denotes |
Strain |
T5335 |
1408-1411 |
NN |
denotes |
SvJ |
T5336 |
1402-1407 |
NN |
denotes |
129Sv |
T5337 |
1407-1408 |
HYPH |
denotes |
/ |
T5338 |
1412-1419 |
JJ |
denotes |
genetic |
T5339 |
1430-1431 |
. |
denotes |
. |
R1315 |
T5031 |
T5032 |
det |
A,subclone |
R1316 |
T5032 |
T5035 |
nsubjpass |
subclone,obtained |
R1317 |
T5033 |
T5032 |
amod |
genomic,subclone |
R1318 |
T5034 |
T5032 |
compound |
DNA,subclone |
R1319 |
T5036 |
T5032 |
prep |
of,subclone |
R1320 |
T5037 |
T5038 |
nmod |
Strain,DNA |
R1321 |
T5038 |
T5036 |
pobj |
DNA,of |
R1322 |
T5039 |
T5040 |
nummod |
129,SvJ |
R1323 |
T5040 |
T5038 |
compound |
SvJ,DNA |
R1324 |
T5041 |
T5040 |
punct |
/,SvJ |
R1325 |
T5042 |
T5043 |
dep |
that,contained |
R1326 |
T5043 |
T5032 |
relcl |
contained,subclone |
R1327 |
T5044 |
T5045 |
det |
a,portion |
R1328 |
T5045 |
T5043 |
dobj |
portion,contained |
R1329 |
T5046 |
T5045 |
prep |
of,portion |
R1330 |
T5047 |
T5048 |
nmod |
Exons,4 |
R1331 |
T5048 |
T5046 |
pobj |
4,of |
R1332 |
T5049 |
T5048 |
cc |
and,4 |
R1333 |
T5050 |
T5048 |
conj |
5,4 |
R1334 |
T5051 |
T5048 |
prep |
of,4 |
R1335 |
T5052 |
T5053 |
det |
the,gene |
R1336 |
T5053 |
T5051 |
pobj |
gene,of |
R1337 |
T5054 |
T5053 |
compound |
E2,gene |
R1338 |
T5055 |
T5053 |
cc |
and,gene |
R1339 |
T5056 |
T5057 |
compound |
flanking,DNA |
R1340 |
T5057 |
T5053 |
conj |
DNA,gene |
R1341 |
T5058 |
T5035 |
auxpass |
was,obtained |
R1342 |
T5059 |
T5035 |
prep |
from,obtained |
R1343 |
T5060 |
T5061 |
det |
a,library |
R1344 |
T5061 |
T5059 |
pobj |
library,from |
R1345 |
T5062 |
T5063 |
compound |
P1,phage |
R1346 |
T5063 |
T5061 |
compound |
phage,library |
R1347 |
T5064 |
T5061 |
prep |
from,library |
R1348 |
T5065 |
T5066 |
nmod |
Genome,Inc. |
R1349 |
T5066 |
T5064 |
pobj |
Inc.,from |
R1350 |
T5067 |
T5066 |
nmod |
Systems,Inc. |
R1351 |
T5068 |
T5066 |
punct |
", ",Inc. |
R1352 |
T5069 |
T5066 |
punct |
", ",Inc. |
R1353 |
T5070 |
T5071 |
punct |
(,Library |
R1354 |
T5071 |
T5066 |
poss |
Library,Inc. |
R1355 |
T5072 |
T5073 |
compound |
St.,Louis |
R1356 |
T5073 |
T5071 |
dep |
Louis,Library |
R1357 |
T5074 |
T5073 |
punct |
", ",Louis |
R1358 |
T5075 |
T5073 |
npadvmod |
MO,Louis |
R1359 |
T5076 |
T5071 |
punct |
;,Library |
R1360 |
T5077 |
T5071 |
punct |
',Library |
R1361 |
T5078 |
T5079 |
nummod |
3,Hit |
R1362 |
T5079 |
T5071 |
compound |
Hit,Library |
R1363 |
T5080 |
T5079 |
punct |
-,Hit |
R1364 |
T5081 |
T5082 |
compound |
Mouse,ES |
R1365 |
T5082 |
T5071 |
compound |
ES,Library |
R1366 |
T5083 |
T5071 |
dep |
',Library |
R1367 |
T5084 |
T5071 |
punct |
),Library |
R1368 |
T5085 |
T5035 |
punct |
.,obtained |
R1369 |
T5087 |
T5088 |
det |
A,vector |
R1370 |
T5088 |
T5096 |
nsubjpass |
vector,created |
R1371 |
T5089 |
T5090 |
amod |
positive,negative |
R1372 |
T5090 |
T5088 |
amod |
negative,vector |
R1373 |
T5091 |
T5090 |
punct |
/,negative |
R1374 |
T5092 |
T5093 |
compound |
replacement,type |
R1375 |
T5093 |
T5088 |
compound |
type,vector |
R1376 |
T5094 |
T5095 |
compound |
gene,targeting |
R1377 |
T5095 |
T5088 |
compound |
targeting,vector |
R1378 |
T5097 |
T5098 |
punct |
[,21 |
R1379 |
T5098 |
T5088 |
parataxis |
21,vector |
R1380 |
T5099 |
T5098 |
punct |
],21 |
R1381 |
T5100 |
T5096 |
auxpass |
was,created |
R1382 |
T5101 |
T5096 |
prep |
by,created |
R1383 |
T5102 |
T5101 |
pcomp |
replacing,by |
R1384 |
T5103 |
T5104 |
det |
a,fragment |
R1385 |
T5104 |
T5102 |
dobj |
fragment,replacing |
R1386 |
T5105 |
T5106 |
nummod |
1.67,kb |
R1387 |
T5106 |
T5104 |
compound |
kb,fragment |
R1388 |
T5107 |
T5108 |
compound |
EcoRV,Smal |
R1389 |
T5108 |
T5104 |
compound |
Smal,fragment |
R1390 |
T5109 |
T5108 |
punct |
-,Smal |
R1391 |
T5110 |
T5111 |
dep |
that,included |
R1392 |
T5111 |
T5104 |
relcl |
included,fragment |
R1393 |
T5112 |
T5113 |
det |
a,portion |
R1394 |
T5113 |
T5111 |
dobj |
portion,included |
R1395 |
T5114 |
T5113 |
prep |
of,portion |
R1396 |
T5115 |
T5114 |
pobj |
Exon,of |
R1397 |
T5116 |
T5115 |
nummod |
4,Exon |
R1398 |
T5117 |
T5113 |
cc |
and,portion |
R1399 |
T5118 |
T5113 |
conj |
all,portion |
R1400 |
T5119 |
T5118 |
prep |
of,all |
R1401 |
T5120 |
T5119 |
pobj |
Exon,of |
R1402 |
T5121 |
T5120 |
nummod |
5,Exon |
R1403 |
T5122 |
T5113 |
prep |
with,portion |
R1404 |
T5123 |
T5124 |
det |
the,gene |
R1405 |
T5124 |
T5122 |
pobj |
gene,with |
R1406 |
T5125 |
T5124 |
compound |
PGKneo,gene |
R1407 |
T5126 |
T5124 |
compound |
marker,gene |
R1408 |
T5127 |
T5128 |
punct |
(,See |
R1409 |
T5128 |
T5124 |
parataxis |
See,gene |
R1410 |
T5129 |
T5130 |
compound |
Fig.,1A |
R1411 |
T5130 |
T5128 |
dobj |
1A,See |
R1412 |
T5131 |
T5128 |
punct |
),See |
R1413 |
T5132 |
T5124 |
prep |
from,gene |
R1414 |
T5133 |
T5134 |
det |
the,vector |
R1415 |
T5134 |
T5132 |
pobj |
vector,from |
R1416 |
T5135 |
T5134 |
compound |
pPNT,vector |
R1417 |
T5136 |
T5137 |
punct |
[,22 |
R1418 |
T5137 |
T5111 |
parataxis |
22,included |
R1419 |
T5138 |
T5137 |
punct |
],22 |
R1420 |
T5139 |
T5096 |
punct |
.,created |
R1421 |
T5141 |
T5142 |
det |
The,construct |
R1422 |
T5142 |
T5144 |
nsubjpass |
construct,linearized |
R1423 |
T5143 |
T5142 |
compound |
targeting,construct |
R1424 |
T5145 |
T5144 |
auxpass |
was,linearized |
R1425 |
T5146 |
T5144 |
prep |
with,linearized |
R1426 |
T5147 |
T5146 |
pobj |
NotI,with |
R1427 |
T5148 |
T5144 |
cc |
and,linearized |
R1428 |
T5149 |
T5144 |
conj |
electroporated,linearized |
R1429 |
T5150 |
T5149 |
prep |
into,electroporated |
R1430 |
T5151 |
T5152 |
compound |
R1,cells |
R1431 |
T5152 |
T5150 |
pobj |
cells,into |
R1432 |
T5153 |
T5152 |
compound |
ES,cells |
R1433 |
T5154 |
T5155 |
punct |
[,23 |
R1434 |
T5155 |
T5152 |
parataxis |
23,cells |
R1435 |
T5156 |
T5155 |
punct |
],23 |
R1436 |
T5157 |
T5149 |
prep |
under,electroporated |
R1437 |
T5158 |
T5157 |
pobj |
conditions,under |
R1438 |
T5159 |
T5158 |
acl |
described,conditions |
R1439 |
T5160 |
T5159 |
advmod |
previously,described |
R1440 |
T5161 |
T5162 |
punct |
[,24 |
R1441 |
T5162 |
T5159 |
parataxis |
24,described |
R1442 |
T5163 |
T5162 |
punct |
],24 |
R1443 |
T5164 |
T5144 |
punct |
.,linearized |
R1444 |
T5166 |
T5167 |
compound |
ES,cells |
R1445 |
T5167 |
T5168 |
nsubjpass |
cells,selected |
R1446 |
T5169 |
T5168 |
auxpass |
were,selected |
R1447 |
T5170 |
T5168 |
prep |
with,selected |
R1448 |
T5171 |
T5170 |
pobj |
G418,with |
R1449 |
T5172 |
T5173 |
punct |
(,Inc. |
R1450 |
T5173 |
T5171 |
parataxis |
Inc.,G418 |
R1451 |
T5174 |
T5175 |
nummod |
300,μg |
R1452 |
T5175 |
T5173 |
dep |
μg,Inc. |
R1453 |
T5176 |
T5177 |
punct |
/,ml |
R1454 |
T5177 |
T5175 |
prep |
ml,μg |
R1455 |
T5178 |
T5173 |
punct |
;,Inc. |
R1456 |
T5179 |
T5173 |
compound |
Life,Inc. |
R1457 |
T5180 |
T5173 |
compound |
Technologies,Inc. |
R1458 |
T5181 |
T5173 |
punct |
", ",Inc. |
R1459 |
T5182 |
T5173 |
npadvmod |
Gaithersburg,Inc. |
R1460 |
T5183 |
T5173 |
punct |
", ",Inc. |
R1461 |
T5184 |
T5173 |
npadvmod |
MD,Inc. |
R1462 |
T5185 |
T5173 |
punct |
),Inc. |
R1463 |
T5186 |
T5171 |
cc |
and,G418 |
R1464 |
T5187 |
T5171 |
conj |
gancyclovir,G418 |
R1465 |
T5188 |
T5187 |
punct |
(,gancyclovir |
R1466 |
T5189 |
T5190 |
nummod |
2,μM |
R1467 |
T5190 |
T5187 |
nmod |
μM,gancyclovir |
R1468 |
T5191 |
T5187 |
punct |
;,gancyclovir |
R1469 |
T5192 |
T5187 |
nmod |
gift,gancyclovir |
R1470 |
T5193 |
T5192 |
prep |
of,gift |
R1471 |
T5194 |
T5193 |
pobj |
Syntex,of |
R1472 |
T5195 |
T5194 |
punct |
", ",Syntex |
R1473 |
T5196 |
T5197 |
compound |
Palo,Alto |
R1474 |
T5197 |
T5194 |
npadvmod |
Alto,Syntex |
R1475 |
T5198 |
T5194 |
punct |
", ",Syntex |
R1476 |
T5199 |
T5194 |
npadvmod |
CA,Syntex |
R1477 |
T5200 |
T5187 |
punct |
),gancyclovir |
R1478 |
T5201 |
T5168 |
punct |
.,selected |
R1479 |
T5203 |
T5204 |
advmod |
Doubly,resistant |
R1480 |
T5204 |
T5205 |
amod |
resistant,clones |
R1481 |
T5205 |
T5206 |
nsubjpass |
clones,screened |
R1482 |
T5207 |
T5206 |
auxpass |
were,screened |
R1483 |
T5208 |
T5206 |
prep |
for,screened |
R1484 |
T5209 |
T5210 |
compound |
gene,targeting |
R1485 |
T5210 |
T5208 |
pobj |
targeting,for |
R1486 |
T5211 |
T5206 |
prep |
by,screened |
R1487 |
T5212 |
T5213 |
compound |
Southern,blot |
R1488 |
T5213 |
T5214 |
compound |
blot,analysis |
R1489 |
T5214 |
T5211 |
pobj |
analysis,by |
R1490 |
T5215 |
T5214 |
prep |
of,analysis |
R1491 |
T5216 |
T5217 |
nmod |
BglI,DNA |
R1492 |
T5217 |
T5215 |
pobj |
DNA,of |
R1493 |
T5218 |
T5217 |
amod |
digested,DNA |
R1494 |
T5219 |
T5217 |
amod |
genomic,DNA |
R1495 |
T5220 |
T5206 |
punct |
.,screened |
R1496 |
T5222 |
T5223 |
nsubjpass |
Blots,hybridized |
R1497 |
T5224 |
T5223 |
auxpass |
were,hybridized |
R1498 |
T5225 |
T5223 |
prep |
with,hybridized |
R1499 |
T5226 |
T5227 |
det |
an,probe |
R1500 |
T5227 |
T5225 |
pobj |
probe,with |
R1501 |
T5228 |
T5229 |
npadvmod |
Exon,specific |
R1502 |
T5229 |
T5227 |
amod |
specific,probe |
R1503 |
T5230 |
T5228 |
nummod |
6,Exon |
R1504 |
T5231 |
T5232 |
dep |
that,was |
R1505 |
T5232 |
T5227 |
relcl |
was,probe |
R1506 |
T5233 |
T5232 |
acomp |
external,was |
R1507 |
T5234 |
T5233 |
prep |
to,external |
R1508 |
T5235 |
T5236 |
det |
the,construct |
R1509 |
T5236 |
T5234 |
pobj |
construct,to |
R1510 |
T5237 |
T5238 |
compound |
gene,targeting |
R1511 |
T5238 |
T5236 |
compound |
targeting,construct |
R1512 |
T5239 |
T5223 |
punct |
.,hybridized |
R1513 |
T5241 |
T5242 |
advmod |
Correctly,targeted |
R1514 |
T5242 |
T5243 |
amod |
targeted,cells |
R1515 |
T5243 |
T5245 |
nsubjpass |
cells,injected |
R1516 |
T5244 |
T5243 |
compound |
ES,cells |
R1517 |
T5246 |
T5245 |
auxpass |
were,injected |
R1518 |
T5247 |
T5245 |
prep |
into,injected |
R1519 |
T5248 |
T5249 |
compound |
C57BL,6J |
R1520 |
T5249 |
T5251 |
compound |
6J,blastocysts |
R1521 |
T5250 |
T5249 |
punct |
/,6J |
R1522 |
T5251 |
T5247 |
pobj |
blastocysts,into |
R1523 |
T5252 |
T5253 |
aux |
to,produce |
R1524 |
T5253 |
T5245 |
advcl |
produce,injected |
R1525 |
T5254 |
T5255 |
amod |
chimeric,mice |
R1526 |
T5255 |
T5253 |
dobj |
mice,produce |
R1527 |
T5256 |
T5253 |
advcl |
using,produce |
R1528 |
T5257 |
T5258 |
amod |
standard,procedures |
R1529 |
T5258 |
T5256 |
dobj |
procedures,using |
R1530 |
T5259 |
T5245 |
punct |
.,injected |
R1531 |
T5261 |
T5262 |
amod |
Heterozygous,offspring |
R1532 |
T5262 |
T5263 |
nsubjpass |
offspring,intercrossed |
R1533 |
T5264 |
T5262 |
prep |
from,offspring |
R1534 |
T5265 |
T5266 |
npadvmod |
germline,competent |
R1535 |
T5266 |
T5267 |
amod |
competent,chimeras |
R1536 |
T5267 |
T5264 |
pobj |
chimeras,from |
R1537 |
T5268 |
T5263 |
auxpass |
were,intercrossed |
R1538 |
T5269 |
T5270 |
aux |
to,produce |
R1539 |
T5270 |
T5263 |
advcl |
produce,intercrossed |
R1540 |
T5271 |
T5272 |
amod |
wild,type |
R1541 |
T5272 |
T5273 |
nmod |
type,knockout |
R1542 |
T5273 |
T5288 |
nmod |
knockout,mice |
R1543 |
T5274 |
T5275 |
punct |
(,+ |
R1544 |
T5275 |
T5272 |
punct |
+,type |
R1545 |
T5276 |
T5275 |
punct |
+,+ |
R1546 |
T5277 |
T5275 |
punct |
/,+ |
R1547 |
T5278 |
T5275 |
punct |
),+ |
R1548 |
T5279 |
T5272 |
punct |
", ",type |
R1549 |
T5280 |
T5272 |
conj |
heterozygous,type |
R1550 |
T5281 |
T5282 |
punct |
(,- |
R1551 |
T5282 |
T5280 |
punct |
-,heterozygous |
R1552 |
T5283 |
T5282 |
punct |
+,- |
R1553 |
T5284 |
T5282 |
punct |
/,- |
R1554 |
T5285 |
T5282 |
punct |
),- |
R1555 |
T5286 |
T5280 |
cc |
and,heterozygous |
R1556 |
T5287 |
T5280 |
conj |
homozygous,heterozygous |
R1557 |
T5288 |
T5270 |
dobj |
mice,produce |
R1558 |
T5289 |
T5290 |
punct |
(,- |
R1559 |
T5290 |
T5273 |
punct |
-,knockout |
R1560 |
T5291 |
T5290 |
punct |
-,- |
R1561 |
T5292 |
T5290 |
punct |
/,- |
R1562 |
T5293 |
T5290 |
punct |
),- |
R1563 |
T5294 |
T5263 |
punct |
.,intercrossed |
R1564 |
T5296 |
T5297 |
prep |
At,used |
R1565 |
T5298 |
T5299 |
det |
all,generations |
R1566 |
T5299 |
T5296 |
pobj |
generations,At |
R1567 |
T5300 |
T5297 |
punct |
", ",used |
R1568 |
T5301 |
T5302 |
punct |
+,- |
R1569 |
T5302 |
T5304 |
punct |
-,pairs |
R1570 |
T5303 |
T5302 |
punct |
/,- |
R1571 |
T5304 |
T5297 |
nsubjpass |
pairs,used |
R1572 |
T5305 |
T5304 |
amod |
breeding,pairs |
R1573 |
T5306 |
T5297 |
auxpass |
were,used |
R1574 |
T5307 |
T5297 |
punct |
.,used |
R1575 |
T5309 |
T5310 |
nsubj |
Results,are |
R1576 |
T5311 |
T5309 |
acl |
presented,Results |
R1577 |
T5312 |
T5311 |
advmod |
here,presented |
R1578 |
T5313 |
T5310 |
prep |
from,are |
R1579 |
T5314 |
T5313 |
pobj |
mice,from |
R1580 |
T5315 |
T5314 |
acl |
derived,mice |
R1581 |
T5316 |
T5315 |
prep |
from,derived |
R1582 |
T5317 |
T5318 |
det |
the,generations |
R1583 |
T5318 |
T5316 |
pobj |
generations,from |
R1584 |
T5319 |
T5318 |
nmod |
F2,generations |
R1585 |
T5320 |
T5319 |
punct |
+,F2 |
R1586 |
T5321 |
T5310 |
punct |
.,are |
R1587 |
T5323 |
T5324 |
det |
All,animals |
R1588 |
T5324 |
T5325 |
nsubj |
animals,were |
R1589 |
T5326 |
T5325 |
prep |
of,were |
R1590 |
T5327 |
T5328 |
det |
a,background |
R1591 |
T5328 |
T5326 |
pobj |
background,of |
R1592 |
T5329 |
T5328 |
amod |
mixed,background |
R1593 |
T5330 |
T5331 |
nmod |
C57BL,6J |
R1594 |
T5331 |
T5328 |
nmod |
6J,background |
R1595 |
T5332 |
T5331 |
punct |
/,6J |
R1596 |
T5333 |
T5331 |
punct |
×,6J |
R1597 |
T5334 |
T5335 |
compound |
Strain,SvJ |
R1598 |
T5335 |
T5331 |
appos |
SvJ,6J |
R1599 |
T5336 |
T5335 |
compound |
129Sv,SvJ |
R1600 |
T5337 |
T5335 |
punct |
/,SvJ |
R1601 |
T5338 |
T5328 |
amod |
genetic,background |
R1602 |
T5339 |
T5325 |
punct |
.,were |