PMC:1448208 / 521-964 JSONTXT

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    craft-sa-dev

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create a murine model of classic MSUD, we used gene targeting and embryonic stem cell technologies to create a mouse line that lacked a functional E2 subunit gene of branched-chain keto acid dehydrogenase. To create a murine model of intermediate MSUD, we used transgenic technology to express a human E2 cDNA on the knockout background. Mice of both models were characterized at the molecular, biochemical, and whole animal levels."}

    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T782","span":{"begin":20,"end":26},"obj":"NCBITaxon:39107"},{"id":"T783","span":{"begin":58,"end":62},"obj":"SO_EXT:0000704"},{"id":"T784","span":{"begin":77,"end":86},"obj":"UBERON:0000922"},{"id":"T785","span":{"begin":77,"end":96},"obj":"CL:0002322"},{"id":"T786","span":{"begin":92,"end":96},"obj":"CL_GO_EXT:cell"},{"id":"T787","span":{"begin":122,"end":127},"obj":"NCBITaxon:10088"},{"id":"T788","span":{"begin":158,"end":168},"obj":"PR_EXT:000006300"},{"id":"T789","span":{"begin":169,"end":173},"obj":"SO_EXT:0000704"},{"id":"T790","span":{"begin":192,"end":201},"obj":"CHEBI:25754"},{"id":"T791","span":{"begin":229,"end":235},"obj":"NCBITaxon:39107"},{"id":"T792","span":{"begin":272,"end":282},"obj":"SO_EXT:transgenic_entity"},{"id":"T793","span":{"begin":297,"end":304},"obj":"GO:0010467"},{"id":"T794","span":{"begin":307,"end":312},"obj":"NCBITaxon:9606"},{"id":"T795","span":{"begin":313,"end":315},"obj":"PR_EXT:000006300"},{"id":"T796","span":{"begin":316,"end":320},"obj":"SO_EXT:cDNA"},{"id":"T797","span":{"begin":349,"end":353},"obj":"NCBITaxon:10088"},{"id":"T798","span":{"begin":395,"end":404},"obj":"CHEBI_EXT:polyatomic_entity_or_group"},{"id":"T799","span":{"begin":406,"end":417},"obj":"CHEBI_EXT:biochemical"},{"id":"T800","span":{"begin":429,"end":435},"obj":"NCBITaxon:33208"}],"text":"Methods\nTo create a murine model of classic MSUD, we used gene targeting and embryonic stem cell technologies to create a mouse line that lacked a functional E2 subunit gene of branched-chain keto acid dehydrogenase. To create a murine model of intermediate MSUD, we used transgenic technology to express a human E2 cDNA on the knockout background. Mice of both models were characterized at the molecular, biochemical, and whole animal levels."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T734","span":{"begin":20,"end":26},"obj":"NCBITaxon:39107"},{"id":"T735","span":{"begin":58,"end":62},"obj":"SO:0000704"},{"id":"T736","span":{"begin":77,"end":86},"obj":"UBERON:0000922"},{"id":"T737","span":{"begin":77,"end":96},"obj":"CL:0002322"},{"id":"T738","span":{"begin":122,"end":127},"obj":"NCBITaxon:10088"},{"id":"T739","span":{"begin":158,"end":168},"obj":"PR:000006300"},{"id":"T740","span":{"begin":169,"end":173},"obj":"SO:0000704"},{"id":"T741","span":{"begin":192,"end":201},"obj":"CHEBI:25754"},{"id":"T742","span":{"begin":229,"end":235},"obj":"NCBITaxon:39107"},{"id":"T743","span":{"begin":297,"end":304},"obj":"GO:0010467"},{"id":"T744","span":{"begin":307,"end":312},"obj":"NCBITaxon:9606"},{"id":"T745","span":{"begin":313,"end":315},"obj":"PR:000006300"},{"id":"T746","span":{"begin":349,"end":353},"obj":"NCBITaxon:10088"},{"id":"T747","span":{"begin":429,"end":435},"obj":"NCBITaxon:33208"}],"text":"Methods\nTo create a murine model of classic MSUD, we used gene targeting and embryonic stem cell technologies to create a mouse line that lacked a functional E2 subunit gene of branched-chain keto acid dehydrogenase. To create a murine model of intermediate MSUD, we used transgenic technology to express a human E2 cDNA on the knockout background. Mice of both models were characterized at the molecular, biochemical, and whole animal levels."}