Id |
Subject |
Object |
Predicate |
Lexical cue |
T926 |
0-7 |
NNS |
denotes |
Methods |
T927 |
7-216 |
sentence |
denotes |
To create a murine model of classic MSUD, we used gene targeting and embryonic stem cell technologies to create a mouse line that lacked a functional E2 subunit gene of branched-chain keto acid dehydrogenase. |
T928 |
8-10 |
TO |
denotes |
To |
T929 |
11-17 |
VB |
denotes |
create |
T930 |
53-57 |
VBD |
denotes |
used |
T931 |
18-19 |
DT |
denotes |
a |
T932 |
27-32 |
NN |
denotes |
model |
T933 |
20-26 |
JJ |
denotes |
murine |
T934 |
33-35 |
IN |
denotes |
of |
T935 |
36-43 |
JJ |
denotes |
classic |
T936 |
44-48 |
NN |
denotes |
MSUD |
T937 |
48-50 |
, |
denotes |
, |
T938 |
50-52 |
PRP |
denotes |
we |
T939 |
58-62 |
NN |
denotes |
gene |
T940 |
63-72 |
NN |
denotes |
targeting |
T941 |
73-76 |
CC |
denotes |
and |
T942 |
77-86 |
JJ |
denotes |
embryonic |
T943 |
92-96 |
NN |
denotes |
cell |
T944 |
87-91 |
NN |
denotes |
stem |
T945 |
97-109 |
NNS |
denotes |
technologies |
T946 |
110-112 |
TO |
denotes |
to |
T947 |
113-119 |
VB |
denotes |
create |
T948 |
120-121 |
DT |
denotes |
a |
T949 |
128-132 |
NN |
denotes |
line |
T950 |
122-127 |
NN |
denotes |
mouse |
T951 |
133-137 |
WDT |
denotes |
that |
T952 |
138-144 |
VBD |
denotes |
lacked |
T953 |
145-146 |
DT |
denotes |
a |
T954 |
169-173 |
NN |
denotes |
gene |
T955 |
147-157 |
JJ |
denotes |
functional |
T956 |
158-160 |
NN |
denotes |
E2 |
T957 |
161-168 |
NN |
denotes |
subunit |
T958 |
174-176 |
IN |
denotes |
of |
T959 |
177-185 |
VBN |
denotes |
branched |
T960 |
186-191 |
NN |
denotes |
chain |
T961 |
185-186 |
HYPH |
denotes |
- |
T962 |
202-215 |
NN |
denotes |
dehydrogenase |
T963 |
192-196 |
NN |
denotes |
keto |
T964 |
197-201 |
NN |
denotes |
acid |
T965 |
215-216 |
. |
denotes |
. |
T966 |
216-348 |
sentence |
denotes |
To create a murine model of intermediate MSUD, we used transgenic technology to express a human E2 cDNA on the knockout background. |
T967 |
217-219 |
TO |
denotes |
To |
T968 |
220-226 |
VB |
denotes |
create |
T969 |
267-271 |
VBD |
denotes |
used |
T970 |
227-228 |
DT |
denotes |
a |
T971 |
236-241 |
NN |
denotes |
model |
T972 |
229-235 |
JJ |
denotes |
murine |
T973 |
242-244 |
IN |
denotes |
of |
T974 |
245-257 |
JJ |
denotes |
intermediate |
T975 |
258-262 |
NN |
denotes |
MSUD |
T976 |
262-264 |
, |
denotes |
, |
T977 |
264-266 |
PRP |
denotes |
we |
T978 |
272-282 |
JJ |
denotes |
transgenic |
T979 |
283-293 |
NN |
denotes |
technology |
T980 |
294-296 |
TO |
denotes |
to |
T981 |
297-304 |
VB |
denotes |
express |
T982 |
305-306 |
DT |
denotes |
a |
T983 |
316-320 |
NN |
denotes |
cDNA |
T984 |
307-312 |
JJ |
denotes |
human |
T985 |
313-315 |
NN |
denotes |
E2 |
T986 |
321-323 |
IN |
denotes |
on |
T987 |
324-327 |
DT |
denotes |
the |
T988 |
337-347 |
NN |
denotes |
background |
T989 |
328-336 |
NN |
denotes |
knockout |
T990 |
347-348 |
. |
denotes |
. |
T991 |
348-443 |
sentence |
denotes |
Mice of both models were characterized at the molecular, biochemical, and whole animal levels. |
T992 |
349-353 |
NNS |
denotes |
Mice |
T993 |
374-387 |
VBN |
denotes |
characterized |
T994 |
354-356 |
IN |
denotes |
of |
T995 |
357-361 |
DT |
denotes |
both |
T996 |
362-368 |
NNS |
denotes |
models |
T997 |
369-373 |
VBD |
denotes |
were |
T998 |
388-390 |
IN |
denotes |
at |
T999 |
391-394 |
DT |
denotes |
the |
T1000 |
436-442 |
NNS |
denotes |
levels |
T1001 |
395-404 |
JJ |
denotes |
molecular |
T1002 |
404-406 |
, |
denotes |
, |
T1003 |
406-417 |
JJ |
denotes |
biochemical |
T1004 |
417-419 |
, |
denotes |
, |
T1005 |
419-422 |
CC |
denotes |
and |
T1006 |
423-428 |
JJ |
denotes |
whole |
T1007 |
429-435 |
NN |
denotes |
animal |
T1008 |
442-443 |
. |
denotes |
. |
R78 |
T928 |
T929 |
aux |
To,create |
R79 |
T929 |
T930 |
advcl |
create,used |
R80 |
T931 |
T932 |
det |
a,model |
R81 |
T932 |
T929 |
dobj |
model,create |
R83 |
T934 |
T932 |
prep |
of,model |
R84 |
T935 |
T936 |
amod |
classic,MSUD |
R85 |
T936 |
T934 |
pobj |
MSUD,of |
R86 |
T937 |
T930 |
punct |
", ",used |
R87 |
T938 |
T930 |
nsubj |
we,used |
R88 |
T939 |
T940 |
compound |
gene,targeting |
R89 |
T940 |
T930 |
dobj |
targeting,used |
R90 |
T941 |
T940 |
cc |
and,targeting |
R91 |
T942 |
T943 |
amod |
embryonic,cell |
R92 |
T943 |
T945 |
compound |
cell,technologies |
R94 |
T945 |
T940 |
conj |
technologies,targeting |
R95 |
T946 |
T947 |
aux |
to,create |
R96 |
T947 |
T930 |
advcl |
create,used |
R97 |
T948 |
T949 |
det |
a,line |
R98 |
T949 |
T947 |
dobj |
line,create |
R100 |
T951 |
T952 |
dep |
that,lacked |
R101 |
T952 |
T949 |
relcl |
lacked,line |
R102 |
T953 |
T954 |
det |
a,gene |
R103 |
T954 |
T952 |
dobj |
gene,lacked |
R107 |
T958 |
T954 |
prep |
of,gene |
R108 |
T959 |
T960 |
amod |
branched,chain |
R109 |
T960 |
T962 |
compound |
chain,dehydrogenase |
R111 |
T962 |
T958 |
pobj |
dehydrogenase,of |
R114 |
T965 |
T930 |
punct |
.,used |
R115 |
T967 |
T968 |
aux |
To,create |
R116 |
T968 |
T969 |
advcl |
create,used |
R117 |
T970 |
T971 |
det |
a,model |
R118 |
T971 |
T968 |
dobj |
model,create |
R120 |
T973 |
T971 |
prep |
of,model |
R121 |
T974 |
T975 |
amod |
intermediate,MSUD |
R122 |
T975 |
T973 |
pobj |
MSUD,of |
R123 |
T976 |
T969 |
punct |
", ",used |
R124 |
T977 |
T969 |
nsubj |
we,used |
R125 |
T978 |
T979 |
amod |
transgenic,technology |
R126 |
T979 |
T969 |
dobj |
technology,used |
R127 |
T980 |
T981 |
aux |
to,express |
R128 |
T981 |
T969 |
advcl |
express,used |
R129 |
T982 |
T983 |
det |
a,cDNA |
R130 |
T983 |
T981 |
dobj |
cDNA,express |
R133 |
T986 |
T983 |
prep |
on,cDNA |
R134 |
T987 |
T988 |
det |
the,background |
R135 |
T988 |
T986 |
pobj |
background,on |
R137 |
T990 |
T969 |
punct |
.,used |
R138 |
T992 |
T993 |
nsubjpass |
Mice,characterized |
R139 |
T994 |
T992 |
prep |
of,Mice |
R140 |
T995 |
T996 |
det |
both,models |
R141 |
T996 |
T994 |
pobj |
models,of |
R142 |
T997 |
T993 |
auxpass |
were,characterized |
R143 |
T998 |
T993 |
prep |
at,characterized |
R144 |
T999 |
T1000 |
det |
the,levels |
R145 |
T1000 |
T998 |
pobj |
levels,at |
R153 |
T1008 |
T993 |
punct |
.,characterized |
R82 |
T933 |
T932 |
amod |
murine,model |
R93 |
T944 |
T943 |
compound |
stem,cell |
R99 |
T950 |
T949 |
compound |
mouse,line |
R104 |
T955 |
T954 |
amod |
functional,gene |
R105 |
T956 |
T954 |
compound |
E2,gene |
R106 |
T957 |
T954 |
compound |
subunit,gene |
R110 |
T961 |
T960 |
punct |
-,chain |
R112 |
T963 |
T964 |
compound |
keto,acid |
R113 |
T964 |
T962 |
compound |
acid,dehydrogenase |
R119 |
T972 |
T971 |
amod |
murine,model |
R131 |
T984 |
T983 |
amod |
human,cDNA |
R132 |
T985 |
T983 |
compound |
E2,cDNA |
R136 |
T989 |
T988 |
compound |
knockout,background |
R146 |
T1001 |
T1000 |
amod |
molecular,levels |
R147 |
T1002 |
T1001 |
punct |
", ",molecular |
R148 |
T1003 |
T1001 |
conj |
biochemical,molecular |
R149 |
T1004 |
T1003 |
punct |
", ",biochemical |
R150 |
T1005 |
T1003 |
cc |
and,biochemical |
R151 |
T1006 |
T1007 |
amod |
whole,animal |
R152 |
T1007 |
T1003 |
conj |
animal,biochemical |