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Pronuclear microinjection was used to produce transgenic mice that harbored the TRE-E2 transgene. From injections at the University of Pittsburgh, 2 transgene positive founders were identified that led to the generation of 3 different transgenic lines (Lines A, B, \u0026 D). From the injections at the University of Cincinnati, we obtained 8 transgene positive founders. Subsequent breeding of these founders revealed that many of the animals had multiple transgene insertion sites that segregated. We established a total of 15 different transgenic lines from these founders. Limited resources allowed us to only focus on a total of 9 of these lines. These lines differed substantially in transgene copy number as compared by Southern blot analysis of tail DNA (data not shown)."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T15567","span":{"begin":840,"end":843},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T15566","span":{"begin":835,"end":839},"obj":"UBERON:0002415"},{"id":"T15540","span":{"begin":8,"end":12},"obj":"NCBITaxon:10088"},{"id":"T15539","span":{"begin":0,"end":3},"obj":"PR_EXT:000005308"},{"id":"T15565","span":{"begin":782,"end":786},"obj":"SO_EXT:sequence_copy_entity"},{"id":"T15564","span":{"begin":772,"end":781},"obj":"SO_EXT:0000902"},{"id":"T15563","span":{"begin":621,"end":631},"obj":"SO_EXT:transgenic_entity"},{"id":"T15562","span":{"begin":549,"end":564},"obj":"SO_EXT:0000366"},{"id":"T15561","span":{"begin":539,"end":548},"obj":"SO_EXT:0000902"},{"id":"T15560","span":{"begin":518,"end":525},"obj":"NCBITaxon:33208"},{"id":"T15559","span":{"begin":465,"end":473},"obj":"GO_EXT:breeding"},{"id":"T15558","span":{"begin":425,"end":434},"obj":"SO_EXT:0000902"},{"id":"T15557","span":{"begin":322,"end":332},"obj":"SO_EXT:transgenic_entity"},{"id":"T15556","span":{"begin":236,"end":245},"obj":"SO_EXT:0000902"},{"id":"T15555","span":{"begin":174,"end":183},"obj":"SO_EXT:0000902"},{"id":"T15554","span":{"begin":171,"end":173},"obj":"PR_EXT:000006300"},{"id":"T15553","span":{"begin":144,"end":148},"obj":"NCBITaxon:10088"},{"id":"T15552","span":{"begin":133,"end":143},"obj":"SO_EXT:transgenic_entity"},{"id":"T15551","span":{"begin":87,"end":97},"obj":"GO:0045120"},{"id":"T15550","span":{"begin":51,"end":52},"obj":"NCBITaxon:10088"},{"id":"T15549","span":{"begin":45,"end":46},"obj":"CHEBI_PR_EXT:protein"},{"id":"T15548","span":{"begin":42,"end":43},"obj":"UBERON_EXT:muscle_structure_or_tissue"},{"id":"T15547","span":{"begin":36,"end":37},"obj":"UBERON:0002113"},{"id":"T15546","span":{"begin":35,"end":36},"obj":"NCBITaxon:10088"},{"id":"T15545","span":{"begin":34,"end":35},"obj":"SO_EXT:transgenic_entity"},{"id":"T15544","span":{"begin":33,"end":34},"obj":"NCBITaxon:9606"},{"id":"T15543","span":{"begin":32,"end":33},"obj":"GO:0042571"},{"id":"T15542","span":{"begin":21,"end":22},"obj":"UBERON:0002107"},{"id":"T15541","span":{"begin":18,"end":21},"obj":"CHEBI_PR_EXT:protein"}],"text":"LAP-tTA mice were previously produced and characterized by the Bujard laboratory [34]. Pronuclear microinjection was used to produce transgenic mice that harbored the TRE-E2 transgene. From injections at the University of Pittsburgh, 2 transgene positive founders were identified that led to the generation of 3 different transgenic lines (Lines A, B, \u0026 D). From the injections at the University of Cincinnati, we obtained 8 transgene positive founders. Subsequent breeding of these founders revealed that many of the animals had multiple transgene insertion sites that segregated. We established a total of 15 different transgenic lines from these founders. Limited resources allowed us to only focus on a total of 9 of these lines. These lines differed substantially in transgene copy number as compared by Southern blot analysis of tail DNA (data not shown)."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T15399","span":{"begin":835,"end":839},"obj":"UBERON:0002415"},{"id":"T15398","span":{"begin":772,"end":781},"obj":"SO:0000902"},{"id":"T15397","span":{"begin":549,"end":564},"obj":"SO:0000366"},{"id":"T15396","span":{"begin":539,"end":548},"obj":"SO:0000902"},{"id":"T15395","span":{"begin":518,"end":525},"obj":"NCBITaxon:33208"},{"id":"T15394","span":{"begin":425,"end":434},"obj":"SO:0000902"},{"id":"T15393","span":{"begin":236,"end":245},"obj":"SO:0000902"},{"id":"T15392","span":{"begin":174,"end":183},"obj":"SO:0000902"},{"id":"T15391","span":{"begin":171,"end":173},"obj":"PR:000006300"},{"id":"T15390","span":{"begin":144,"end":148},"obj":"NCBITaxon:10088"},{"id":"T15389","span":{"begin":87,"end":97},"obj":"GO:0045120"},{"id":"T15388","span":{"begin":51,"end":52},"obj":"NCBITaxon:10088"},{"id":"T15387","span":{"begin":36,"end":37},"obj":"UBERON:0002113"},{"id":"T15386","span":{"begin":35,"end":36},"obj":"NCBITaxon:10088"},{"id":"T15385","span":{"begin":33,"end":34},"obj":"NCBITaxon:9606"},{"id":"T15384","span":{"begin":32,"end":33},"obj":"GO:0042571"},{"id":"T15383","span":{"begin":21,"end":22},"obj":"UBERON:0002107"},{"id":"T15382","span":{"begin":8,"end":12},"obj":"NCBITaxon:10088"},{"id":"T15381","span":{"begin":0,"end":3},"obj":"PR:000005308"}],"text":"LAP-tTA mice were previously produced and characterized by the Bujard laboratory [34]. Pronuclear microinjection was used to produce transgenic mice that harbored the TRE-E2 transgene. From injections at the University of Pittsburgh, 2 transgene positive founders were identified that led to the generation of 3 different transgenic lines (Lines A, B, \u0026 D). From the injections at the University of Cincinnati, we obtained 8 transgene positive founders. Subsequent breeding of these founders revealed that many of the animals had multiple transgene insertion sites that segregated. We established a total of 15 different transgenic lines from these founders. Limited resources allowed us to only focus on a total of 9 of these lines. These lines differed substantially in transgene copy number as compared by Southern blot analysis of tail DNA (data not shown)."}