Id |
Subject |
Object |
Predicate |
Lexical cue |
T16088 |
860-861 |
. |
denotes |
. |
T16087 |
859-860 |
-RRB- |
denotes |
) |
T16086 |
850-853 |
RB |
denotes |
not |
T16085 |
845-849 |
NNS |
denotes |
data |
T16084 |
854-859 |
VBN |
denotes |
shown |
T16083 |
844-845 |
-LRB- |
denotes |
( |
T16082 |
840-843 |
NN |
denotes |
DNA |
T16081 |
835-839 |
NN |
denotes |
tail |
T16080 |
832-834 |
IN |
denotes |
of |
T16079 |
823-831 |
NN |
denotes |
analysis |
T16078 |
818-822 |
NN |
denotes |
blot |
T16077 |
809-817 |
NNP |
denotes |
Southern |
T16076 |
806-808 |
IN |
denotes |
by |
T16075 |
797-805 |
VBN |
denotes |
compared |
T16074 |
794-796 |
IN |
denotes |
as |
T16073 |
782-786 |
NN |
denotes |
copy |
T16072 |
787-793 |
NN |
denotes |
number |
T16071 |
772-781 |
NN |
denotes |
transgene |
T16070 |
769-771 |
IN |
denotes |
in |
T16069 |
755-768 |
RB |
denotes |
substantially |
T16068 |
746-754 |
VBD |
denotes |
differed |
T16067 |
740-745 |
NNS |
denotes |
lines |
T16066 |
734-739 |
DT |
denotes |
These |
T16065 |
733-861 |
sentence |
denotes |
These lines differed substantially in transgene copy number as compared by Southern blot analysis of tail DNA (data not shown). |
T16064 |
732-733 |
. |
denotes |
. |
T16063 |
727-732 |
NNS |
denotes |
lines |
T16062 |
721-726 |
DT |
denotes |
these |
T16061 |
718-720 |
IN |
denotes |
of |
T16060 |
716-717 |
CD |
denotes |
9 |
T16059 |
713-715 |
IN |
denotes |
of |
T16058 |
707-712 |
NN |
denotes |
total |
T16057 |
705-706 |
DT |
denotes |
a |
T16056 |
702-704 |
IN |
denotes |
on |
T16055 |
691-695 |
RB |
denotes |
only |
T16054 |
688-690 |
TO |
denotes |
to |
T16053 |
696-701 |
VB |
denotes |
focus |
T16052 |
685-687 |
PRP |
denotes |
us |
T16051 |
677-684 |
VBD |
denotes |
allowed |
T16050 |
667-676 |
NNS |
denotes |
resources |
T16049 |
659-666 |
VBN |
denotes |
Limited |
T16048 |
658-733 |
sentence |
denotes |
Limited resources allowed us to only focus on a total of 9 of these lines. |
T16047 |
657-658 |
. |
denotes |
. |
T16046 |
649-657 |
NNS |
denotes |
founders |
T16045 |
643-648 |
DT |
denotes |
these |
T16044 |
638-642 |
IN |
denotes |
from |
T16043 |
621-631 |
JJ |
denotes |
transgenic |
T16042 |
611-620 |
JJ |
denotes |
different |
T16041 |
632-637 |
NNS |
denotes |
lines |
T16040 |
608-610 |
CD |
denotes |
15 |
T16039 |
605-607 |
IN |
denotes |
of |
T16038 |
599-604 |
NN |
denotes |
total |
T16037 |
597-598 |
DT |
denotes |
a |
T16036 |
585-596 |
VBD |
denotes |
established |
T16035 |
582-584 |
PRP |
denotes |
We |
T16034 |
581-658 |
sentence |
denotes |
We established a total of 15 different transgenic lines from these founders. |
T16033 |
580-581 |
. |
denotes |
. |
T16032 |
570-580 |
VBD |
denotes |
segregated |
T16031 |
565-569 |
WDT |
denotes |
that |
T16030 |
549-558 |
NN |
denotes |
insertion |
T16029 |
539-548 |
NN |
denotes |
transgene |
T16028 |
559-564 |
NNS |
denotes |
sites |
T16027 |
530-538 |
JJ |
denotes |
multiple |
T16026 |
518-525 |
NNS |
denotes |
animals |
T16025 |
514-517 |
DT |
denotes |
the |
T16024 |
511-513 |
IN |
denotes |
of |
T16023 |
506-510 |
JJ |
denotes |
many |
T16022 |
526-529 |
VBD |
denotes |
had |
T16021 |
501-505 |
IN |
denotes |
that |
T16020 |
483-491 |
NNS |
denotes |
founders |
T16019 |
477-482 |
DT |
denotes |
these |
T16018 |
474-476 |
IN |
denotes |
of |
T16017 |
492-500 |
VBD |
denotes |
revealed |
T16016 |
465-473 |
NN |
denotes |
breeding |
T16015 |
454-464 |
JJ |
denotes |
Subsequent |
T16014 |
453-581 |
sentence |
denotes |
Subsequent breeding of these founders revealed that many of the animals had multiple transgene insertion sites that segregated. |
T16013 |
452-453 |
. |
denotes |
. |
T16012 |
435-443 |
JJ |
denotes |
positive |
T16011 |
425-434 |
NN |
denotes |
transgene |
T16010 |
444-452 |
NNS |
denotes |
founders |
T16009 |
423-424 |
CD |
denotes |
8 |
T16008 |
411-413 |
PRP |
denotes |
we |
T16007 |
409-411 |
, |
denotes |
, |
T16006 |
399-409 |
NNP |
denotes |
Cincinnati |
T16005 |
396-398 |
IN |
denotes |
of |
T16004 |
385-395 |
NNP |
denotes |
University |
T16003 |
381-384 |
DT |
denotes |
the |
T16002 |
378-380 |
IN |
denotes |
at |
T16001 |
367-377 |
NNS |
denotes |
injections |
T16000 |
363-366 |
DT |
denotes |
the |
T15999 |
414-422 |
VBD |
denotes |
obtained |
T15998 |
358-362 |
IN |
denotes |
From |
T15997 |
357-453 |
sentence |
denotes |
From the injections at the University of Cincinnati, we obtained 8 transgene positive founders. |
T15996 |
356-357 |
. |
denotes |
. |
T15995 |
355-356 |
-RRB- |
denotes |
) |
T15994 |
354-355 |
NN |
denotes |
D |
T15993 |
352-353 |
CC |
denotes |
& |
T15992 |
350-352 |
, |
denotes |
, |
T15991 |
349-350 |
NN |
denotes |
B |
T15990 |
347-349 |
, |
denotes |
, |
T15989 |
340-345 |
NNS |
denotes |
Lines |
T15988 |
346-347 |
NN |
denotes |
A |
T15987 |
339-340 |
-LRB- |
denotes |
( |
T15986 |
322-332 |
JJ |
denotes |
transgenic |
T15985 |
312-321 |
JJ |
denotes |
different |
T15984 |
333-338 |
NNS |
denotes |
lines |
T15983 |
310-311 |
CD |
denotes |
3 |
T15982 |
307-309 |
IN |
denotes |
of |
T15981 |
296-306 |
NN |
denotes |
generation |
T15980 |
292-295 |
DT |
denotes |
the |
T15979 |
289-291 |
IN |
denotes |
to |
T15978 |
285-288 |
VBD |
denotes |
led |
T15977 |
280-284 |
WDT |
denotes |
that |
T15976 |
264-268 |
VBD |
denotes |
were |
T15975 |
246-254 |
JJ |
denotes |
positive |
T15974 |
236-245 |
NN |
denotes |
transgene |
T15973 |
255-263 |
NNS |
denotes |
founders |
T15972 |
234-235 |
CD |
denotes |
2 |
T15971 |
232-234 |
, |
denotes |
, |
T15970 |
222-232 |
NNP |
denotes |
Pittsburgh |
T15969 |
219-221 |
IN |
denotes |
of |
T15968 |
208-218 |
NNP |
denotes |
University |
T15967 |
204-207 |
DT |
denotes |
the |
T15966 |
201-203 |
IN |
denotes |
at |
T15965 |
190-200 |
NNS |
denotes |
injections |
T15964 |
269-279 |
VBN |
denotes |
identified |
T15963 |
185-189 |
IN |
denotes |
From |
T15962 |
184-357 |
sentence |
denotes |
From injections at the University of Pittsburgh, 2 transgene positive founders were identified that led to the generation of 3 different transgenic lines (Lines A, B, & D). |
T15961 |
183-184 |
. |
denotes |
. |
T15960 |
170-171 |
HYPH |
denotes |
- |
T15938 |
59-62 |
DT |
denotes |
the |
T15937 |
56-58 |
IN |
denotes |
by |
T15936 |
52-55 |
VBN |
denotes |
zed |
T15935 |
51-52 |
NNS |
denotes |
i |
T15934 |
51-86 |
sentence |
denotes |
ized by the Bujard laboratory [34]. |
T15900 |
8-12 |
NNS |
denotes |
mice |
T15899 |
3-4 |
HYPH |
denotes |
- |
T15898 |
4-7 |
NN |
denotes |
tTA |
T15897 |
0-3 |
NN |
denotes |
LAP |
T15896 |
0-13 |
sentence |
denotes |
LAP-tTA mice |
T15911 |
24-25 |
NN |
denotes |
u |
T15910 |
23-24 |
VB |
denotes |
o |
T15909 |
23-29 |
sentence |
denotes |
ously |
T15908 |
22-23 |
JJ |
denotes |
i |
T15907 |
21-22 |
NN |
denotes |
v |
T15906 |
18-21 |
NN |
denotes |
pre |
T15905 |
18-23 |
sentence |
denotes |
previ |
T15904 |
15-17 |
VB |
denotes |
re |
T15903 |
13-14 |
VBD |
denotes |
w |
T15902 |
14-15 |
VBN |
denotes |
e |
T15901 |
13-18 |
sentence |
denotes |
were |
T15917 |
32-33 |
NN |
denotes |
d |
T15916 |
29-32 |
NN |
denotes |
pro |
T15915 |
29-36 |
sentence |
denotes |
produce |
T15914 |
27-28 |
JJ |
denotes |
y |
T15913 |
26-27 |
JJ |
denotes |
l |
T15912 |
25-26 |
NNS |
denotes |
s |
T15959 |
171-173 |
NN |
denotes |
E2 |
T15958 |
167-170 |
NN |
denotes |
TRE |
T15957 |
174-183 |
NN |
denotes |
transgene |
T15956 |
163-166 |
DT |
denotes |
the |
T15955 |
154-162 |
VBD |
denotes |
harbored |
T15954 |
149-153 |
WDT |
denotes |
that |
T15953 |
144-148 |
NNS |
denotes |
mice |
T15952 |
133-143 |
JJ |
denotes |
transgenic |
T15951 |
125-132 |
VB |
denotes |
produce |
T15950 |
122-124 |
TO |
denotes |
to |
T15949 |
113-116 |
VBD |
denotes |
was |
T15948 |
117-121 |
VBN |
denotes |
used |
T15947 |
98-112 |
NN |
denotes |
microinjection |
T15946 |
87-97 |
JJ |
denotes |
Pronuclear |
T15945 |
86-184 |
sentence |
denotes |
Pronuclear microinjection was used to produce transgenic mice that harbored the TRE-E2 transgene. |
T15944 |
85-86 |
. |
denotes |
. |
T15943 |
84-85 |
-RRB- |
denotes |
] |
T15942 |
82-84 |
CD |
denotes |
34 |
T15941 |
81-82 |
-LRB- |
denotes |
[ |
T15940 |
63-69 |
NNP |
denotes |
Bujard |
T15939 |
70-80 |
NN |
denotes |
laboratory |
T15933 |
49-50 |
VB |
denotes |
e |
T15932 |
48-49 |
TO |
denotes |
t |
T15931 |
47-48 |
DT |
denotes |
c |
T15930 |
46-47 |
VBN |
denotes |
a |
T15929 |
45-46 |
NN |
denotes |
r |
T15928 |
50-51 |
VBN |
denotes |
r |
T15927 |
44-45 |
DT |
denotes |
a |
T15926 |
44-51 |
sentence |
denotes |
aracter |
T15925 |
43-44 |
DT |
denotes |
h |
T15924 |
42-43 |
NN |
denotes |
c |
T15923 |
38-41 |
CC |
denotes |
and |
T15922 |
36-37 |
NN |
denotes |
d |
T15921 |
36-44 |
sentence |
denotes |
d and ch |
T15920 |
35-36 |
NNS |
denotes |
e |
T15919 |
34-35 |
JJ |
denotes |
c |
T15918 |
33-34 |
JJ |
denotes |
u |
R4008 |
T15941 |
T15942 |
punct |
[,34 |
R4009 |
T15942 |
T15936 |
parataxis |
34,zed |
R4010 |
T15943 |
T15942 |
punct |
],34 |
R4011 |
T15946 |
T15947 |
amod |
Pronuclear,microinjection |
R4012 |
T15947 |
T15948 |
nsubjpass |
microinjection,used |
R4013 |
T15949 |
T15948 |
auxpass |
was,used |
R4014 |
T15950 |
T15951 |
aux |
to,produce |
R4015 |
T15951 |
T15948 |
advcl |
produce,used |
R4016 |
T15952 |
T15953 |
amod |
transgenic,mice |
R4017 |
T15953 |
T15951 |
dobj |
mice,produce |
R4018 |
T15954 |
T15955 |
dep |
that,harbored |
R4019 |
T15955 |
T15953 |
relcl |
harbored,mice |
R4020 |
T15956 |
T15957 |
det |
the,transgene |
R4021 |
T15957 |
T15955 |
dobj |
transgene,harbored |
R4022 |
T15958 |
T15959 |
compound |
TRE,E2 |
R4023 |
T15959 |
T15957 |
compound |
E2,transgene |
R4024 |
T15960 |
T15959 |
punct |
-,E2 |
R4025 |
T15961 |
T15948 |
punct |
.,used |
R4026 |
T15963 |
T15964 |
prep |
From,identified |
R4027 |
T15965 |
T15963 |
pobj |
injections,From |
R4028 |
T15966 |
T15965 |
prep |
at,injections |
R4029 |
T15967 |
T15968 |
det |
the,University |
R4030 |
T15968 |
T15966 |
pobj |
University,at |
R4031 |
T15969 |
T15968 |
prep |
of,University |
R4032 |
T15970 |
T15969 |
pobj |
Pittsburgh,of |
R4033 |
T15971 |
T15964 |
punct |
", ",identified |
R4034 |
T15972 |
T15973 |
nummod |
2,founders |
R4035 |
T15973 |
T15964 |
nsubjpass |
founders,identified |
R4036 |
T15974 |
T15975 |
npadvmod |
transgene,positive |
R4037 |
T15975 |
T15973 |
amod |
positive,founders |
R4038 |
T15976 |
T15964 |
auxpass |
were,identified |
R4039 |
T15977 |
T15978 |
dep |
that,led |
R4040 |
T15978 |
T15964 |
ccomp |
led,identified |
R4041 |
T15979 |
T15978 |
prep |
to,led |
R4042 |
T15980 |
T15981 |
det |
the,generation |
R4043 |
T15981 |
T15979 |
pobj |
generation,to |
R4044 |
T15982 |
T15981 |
prep |
of,generation |
R4045 |
T15983 |
T15984 |
nummod |
3,lines |
R4046 |
T15984 |
T15982 |
pobj |
lines,of |
R4047 |
T15985 |
T15984 |
amod |
different,lines |
R4048 |
T15986 |
T15984 |
amod |
transgenic,lines |
R4049 |
T15987 |
T15988 |
punct |
(,A |
R4050 |
T15988 |
T15984 |
parataxis |
A,lines |
R4051 |
T15989 |
T15988 |
compound |
Lines,A |
R4052 |
T15990 |
T15988 |
punct |
", ",A |
R4053 |
T15991 |
T15988 |
conj |
B,A |
R4054 |
T15992 |
T15991 |
punct |
", ",B |
R4055 |
T15993 |
T15991 |
cc |
&,B |
R4056 |
T15994 |
T15991 |
conj |
D,B |
R4057 |
T15995 |
T15988 |
punct |
),A |
R4058 |
T15996 |
T15964 |
punct |
.,identified |
R4059 |
T15998 |
T15999 |
prep |
From,obtained |
R4060 |
T16000 |
T16001 |
det |
the,injections |
R4061 |
T16001 |
T15998 |
pobj |
injections,From |
R4062 |
T16002 |
T16001 |
prep |
at,injections |
R4063 |
T16003 |
T16004 |
det |
the,University |
R4064 |
T16004 |
T16002 |
pobj |
University,at |
R4065 |
T16005 |
T16004 |
prep |
of,University |
R4066 |
T16006 |
T16005 |
pobj |
Cincinnati,of |
R4067 |
T16007 |
T15999 |
punct |
", ",obtained |
R4068 |
T16008 |
T15999 |
nsubj |
we,obtained |
R4069 |
T16009 |
T16010 |
nummod |
8,founders |
R4070 |
T16010 |
T15999 |
dobj |
founders,obtained |
R4071 |
T16011 |
T16012 |
npadvmod |
transgene,positive |
R4072 |
T16012 |
T16010 |
amod |
positive,founders |
R4073 |
T16013 |
T15999 |
punct |
.,obtained |
R4074 |
T16015 |
T16016 |
amod |
Subsequent,breeding |
R4075 |
T16016 |
T16017 |
nsubj |
breeding,revealed |
R4076 |
T16018 |
T16016 |
prep |
of,breeding |
R4077 |
T16019 |
T16020 |
det |
these,founders |
R4078 |
T16020 |
T16018 |
pobj |
founders,of |
R4079 |
T16021 |
T16022 |
mark |
that,had |
R4080 |
T16022 |
T16017 |
ccomp |
had,revealed |
R4081 |
T16023 |
T16022 |
nsubj |
many,had |
R4082 |
T16024 |
T16023 |
prep |
of,many |
R4083 |
T16025 |
T16026 |
det |
the,animals |
R4084 |
T16026 |
T16024 |
pobj |
animals,of |
R4085 |
T16027 |
T16028 |
amod |
multiple,sites |
R4086 |
T16028 |
T16022 |
dobj |
sites,had |
R4087 |
T16029 |
T16028 |
compound |
transgene,sites |
R4088 |
T16030 |
T16028 |
compound |
insertion,sites |
R4089 |
T16031 |
T16032 |
dep |
that,segregated |
R4090 |
T16032 |
T16028 |
relcl |
segregated,sites |
R4091 |
T16033 |
T16017 |
punct |
.,revealed |
R4092 |
T16035 |
T16036 |
nsubj |
We,established |
R4093 |
T16037 |
T16038 |
det |
a,total |
R4094 |
T16038 |
T16036 |
dobj |
total,established |
R4095 |
T16039 |
T16038 |
prep |
of,total |
R4096 |
T16040 |
T16041 |
nummod |
15,lines |
R4097 |
T16041 |
T16039 |
pobj |
lines,of |
R4098 |
T16042 |
T16041 |
amod |
different,lines |
R4099 |
T16043 |
T16041 |
amod |
transgenic,lines |
R4100 |
T16044 |
T16036 |
prep |
from,established |
R4101 |
T16045 |
T16046 |
det |
these,founders |
R4102 |
T16046 |
T16044 |
pobj |
founders,from |
R4103 |
T16047 |
T16036 |
punct |
.,established |
R4104 |
T16049 |
T16050 |
amod |
Limited,resources |
R4105 |
T16050 |
T16051 |
nsubj |
resources,allowed |
R4106 |
T16052 |
T16053 |
nsubj |
us,focus |
R4107 |
T16053 |
T16051 |
ccomp |
focus,allowed |
R4108 |
T16054 |
T16053 |
aux |
to,focus |
R4109 |
T16055 |
T16053 |
advmod |
only,focus |
R4110 |
T16056 |
T16053 |
prep |
on,focus |
R4111 |
T16057 |
T16058 |
det |
a,total |
R4112 |
T16058 |
T16056 |
pobj |
total,on |
R4113 |
T16059 |
T16058 |
prep |
of,total |
R4114 |
T16060 |
T16059 |
pobj |
9,of |
R4115 |
T16061 |
T16060 |
prep |
of,9 |
R4116 |
T16062 |
T16063 |
det |
these,lines |
R4117 |
T16063 |
T16061 |
pobj |
lines,of |
R4118 |
T16064 |
T16051 |
punct |
.,allowed |
R4119 |
T16066 |
T16067 |
det |
These,lines |
R4120 |
T16067 |
T16068 |
nsubj |
lines,differed |
R4121 |
T16069 |
T16068 |
advmod |
substantially,differed |
R4122 |
T16070 |
T16068 |
prep |
in,differed |
R4123 |
T16071 |
T16072 |
compound |
transgene,number |
R4124 |
T16072 |
T16070 |
pobj |
number,in |
R4125 |
T16073 |
T16072 |
compound |
copy,number |
R4126 |
T16074 |
T16075 |
mark |
as,compared |
R4127 |
T16075 |
T16068 |
advcl |
compared,differed |
R4128 |
T16076 |
T16075 |
prep |
by,compared |
R4129 |
T16077 |
T16078 |
compound |
Southern,blot |
R4130 |
T16078 |
T16079 |
compound |
blot,analysis |
R4131 |
T16079 |
T16076 |
pobj |
analysis,by |
R4132 |
T16080 |
T16079 |
prep |
of,analysis |
R4133 |
T16081 |
T16082 |
compound |
tail,DNA |
R4134 |
T16082 |
T16080 |
pobj |
DNA,of |
R4135 |
T16083 |
T16084 |
punct |
(,shown |
R4136 |
T16084 |
T16068 |
parataxis |
shown,differed |
R4137 |
T16085 |
T16084 |
nsubj |
data,shown |
R4138 |
T16086 |
T16084 |
neg |
not,shown |
R4139 |
T16087 |
T16084 |
punct |
),shown |
R4140 |
T16088 |
T16068 |
punct |
.,differed |
R3993 |
T15897 |
T15898 |
nmod |
LAP,tTA |
R3994 |
T15898 |
T15900 |
nmod |
tTA,mice |
R3995 |
T15899 |
T15898 |
punct |
-,tTA |
R3996 |
T15903 |
T15902 |
auxpass |
w,e |
R3997 |
T15904 |
T15902 |
advcl |
re,e |
R3998 |
T15919 |
T15920 |
amod |
c,e |
R3999 |
T15923 |
T15922 |
cc |
and,d |
R4000 |
T15924 |
T15922 |
conj |
c,d |
R4001 |
T15930 |
T15929 |
acl |
a,r |
R4002 |
T15932 |
T15928 |
aux |
t,r |
R4003 |
T15933 |
T15928 |
auxpass |
e,r |
R4004 |
T15937 |
T15936 |
agent |
by,zed |
R4005 |
T15938 |
T15939 |
det |
the,laboratory |
R4006 |
T15939 |
T15937 |
pobj |
laboratory,by |
R4007 |
T15940 |
T15939 |
compound |
Bujard,laboratory |