PMC:1448208 / 12941-13248 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1448208","sourcedb":"PMC","sourceid":"1448208","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1448208","text":" Following a 30 min incubation with purified goat IgG (50 (μg/ml) at 25°C and three additional washes with Buffer A, cells were incubated for 60 min with E2-specific antiserum [30] at 1 μg/ml followed by three washes in Buffer A and 60 minute incubation in fluorescently labeled second antibody (1–2 μg/ml).","tracks":[{"project":"craft-sa-dev","denotations":[{"id":"T7258","span":{"begin":0,"end":307},"obj":"sentence"},{"id":"T7259","span":{"begin":1,"end":10},"obj":"VBG"},{"id":"T7260","span":{"begin":128,"end":137},"obj":"VBN"},{"id":"T7261","span":{"begin":11,"end":12},"obj":"DT"},{"id":"T7262","span":{"begin":20,"end":30},"obj":"NN"},{"id":"T7263","span":{"begin":13,"end":15},"obj":"CD"},{"id":"T7264","span":{"begin":16,"end":19},"obj":"NN"},{"id":"T7265","span":{"begin":31,"end":35},"obj":"IN"},{"id":"T7266","span":{"begin":36,"end":44},"obj":"VBN"},{"id":"T7267","span":{"begin":50,"end":53},"obj":"NN"},{"id":"T7268","span":{"begin":45,"end":49},"obj":"NN"},{"id":"T7269","span":{"begin":54,"end":55},"obj":"-LRB-"},{"id":"T7270","span":{"begin":59,"end":61},"obj":"NN"},{"id":"T7271","span":{"begin":55,"end":57},"obj":"CD"},{"id":"T7272","span":{"begin":58,"end":59},"obj":"-LRB-"},{"id":"T7273","span":{"begin":61,"end":62},"obj":"SYM"},{"id":"T7274","span":{"begin":62,"end":64},"obj":"NN"},{"id":"T7275","span":{"begin":64,"end":65},"obj":"-RRB-"},{"id":"T7276","span":{"begin":66,"end":68},"obj":"IN"},{"id":"T7277","span":{"begin":69,"end":71},"obj":"CD"},{"id":"T7278","span":{"begin":71,"end":73},"obj":"NN"},{"id":"T7279","span":{"begin":74,"end":77},"obj":"CC"},{"id":"T7280","span":{"begin":78,"end":83},"obj":"CD"},{"id":"T7281","span":{"begin":95,"end":101},"obj":"NNS"},{"id":"T7282","span":{"begin":84,"end":94},"obj":"JJ"},{"id":"T7283","span":{"begin":102,"end":106},"obj":"IN"},{"id":"T7284","span":{"begin":107,"end":113},"obj":"NN"},{"id":"T7285","span":{"begin":114,"end":115},"obj":"NN"},{"id":"T7286","span":{"begin":115,"end":117},"obj":","},{"id":"T7287","span":{"begin":117,"end":122},"obj":"NNS"},{"id":"T7288","span":{"begin":123,"end":127},"obj":"VBD"},{"id":"T7289","span":{"begin":138,"end":141},"obj":"IN"},{"id":"T7290","span":{"begin":142,"end":144},"obj":"CD"},{"id":"T7291","span":{"begin":145,"end":148},"obj":"NN"},{"id":"T7292","span":{"begin":149,"end":153},"obj":"IN"},{"id":"T7293","span":{"begin":154,"end":156},"obj":"NN"},{"id":"T7294","span":{"begin":157,"end":165},"obj":"JJ"},{"id":"T7295","span":{"begin":156,"end":157},"obj":"HYPH"},{"id":"T7296","span":{"begin":166,"end":175},"obj":"NN"},{"id":"T7297","span":{"begin":176,"end":177},"obj":"-LRB-"},{"id":"T7298","span":{"begin":177,"end":179},"obj":"CD"},{"id":"T7299","span":{"begin":179,"end":180},"obj":"-RRB-"},{"id":"T7300","span":{"begin":181,"end":183},"obj":"IN"},{"id":"T7301","span":{"begin":184,"end":185},"obj":"CD"},{"id":"T7302","span":{"begin":186,"end":188},"obj":"NN"},{"id":"T7303","span":{"begin":188,"end":189},"obj":"SYM"},{"id":"T7304","span":{"begin":189,"end":191},"obj":"NN"},{"id":"T7305","span":{"begin":192,"end":200},"obj":"VBN"},{"id":"T7306","span":{"begin":201,"end":203},"obj":"IN"},{"id":"T7307","span":{"begin":204,"end":209},"obj":"CD"},{"id":"T7308","span":{"begin":210,"end":216},"obj":"NNS"},{"id":"T7309","span":{"begin":217,"end":219},"obj":"IN"},{"id":"T7310","span":{"begin":220,"end":226},"obj":"NN"},{"id":"T7311","span":{"begin":227,"end":228},"obj":"NN"},{"id":"T7312","span":{"begin":229,"end":232},"obj":"CC"},{"id":"T7313","span":{"begin":233,"end":235},"obj":"CD"},{"id":"T7314","span":{"begin":236,"end":242},"obj":"NN"},{"id":"T7315","span":{"begin":243,"end":253},"obj":"NN"},{"id":"T7316","span":{"begin":254,"end":256},"obj":"IN"},{"id":"T7317","span":{"begin":257,"end":270},"obj":"RB"},{"id":"T7318","span":{"begin":271,"end":278},"obj":"VBN"},{"id":"T7319","span":{"begin":286,"end":294},"obj":"NN"},{"id":"T7320","span":{"begin":279,"end":285},"obj":"JJ"},{"id":"T7321","span":{"begin":295,"end":296},"obj":"-LRB-"},{"id":"T7322","span":{"begin":300,"end":302},"obj":"NN"},{"id":"T7323","span":{"begin":296,"end":297},"obj":"CD"},{"id":"T7324","span":{"begin":298,"end":299},"obj":"CD"},{"id":"T7325","span":{"begin":297,"end":298},"obj":"SYM"},{"id":"T7326","span":{"begin":302,"end":303},"obj":"SYM"},{"id":"T7327","span":{"begin":303,"end":305},"obj":"NN"},{"id":"T7328","span":{"begin":305,"end":306},"obj":"-RRB-"},{"id":"T7329","span":{"begin":306,"end":307},"obj":"."}],"relations":[{"id":"R2079","pred":"prep","subj":"T7259","obj":"T7260"},{"id":"R2080","pred":"det","subj":"T7261","obj":"T7262"},{"id":"R2081","pred":"pobj","subj":"T7262","obj":"T7259"},{"id":"R2082","pred":"nummod","subj":"T7263","obj":"T7264"},{"id":"R2083","pred":"compound","subj":"T7264","obj":"T7262"},{"id":"R2084","pred":"prep","subj":"T7265","obj":"T7262"},{"id":"R2085","pred":"amod","subj":"T7266","obj":"T7267"},{"id":"R2086","pred":"pobj","subj":"T7267","obj":"T7265"},{"id":"R2087","pred":"compound","subj":"T7268","obj":"T7267"},{"id":"R2088","pred":"punct","subj":"T7269","obj":"T7270"},{"id":"R2089","pred":"parataxis","subj":"T7270","obj":"T7267"},{"id":"R2090","pred":"nummod","subj":"T7271","obj":"T7270"},{"id":"R2091","pred":"punct","subj":"T7272","obj":"T7270"},{"id":"R2092","pred":"punct","subj":"T7273","obj":"T7274"},{"id":"R2093","pred":"prep","subj":"T7274","obj":"T7270"},{"id":"R2094","pred":"punct","subj":"T7275","obj":"T7270"},{"id":"R2095","pred":"prep","subj":"T7276","obj":"T7262"},{"id":"R2096","pred":"nummod","subj":"T7277","obj":"T7278"},{"id":"R2097","pred":"pobj","subj":"T7278","obj":"T7276"},{"id":"R2098","pred":"cc","subj":"T7279","obj":"T7262"},{"id":"R2099","pred":"nummod","subj":"T7280","obj":"T7281"},{"id":"R2100","pred":"conj","subj":"T7281","obj":"T7262"},{"id":"R2101","pred":"amod","subj":"T7282","obj":"T7281"},{"id":"R2102","pred":"prep","subj":"T7283","obj":"T7281"},{"id":"R2103","pred":"compound","subj":"T7284","obj":"T7285"},{"id":"R2104","pred":"pobj","subj":"T7285","obj":"T7283"},{"id":"R2105","pred":"punct","subj":"T7286","obj":"T7260"},{"id":"R2106","pred":"nsubjpass","subj":"T7287","obj":"T7260"},{"id":"R2107","pred":"auxpass","subj":"T7288","obj":"T7260"},{"id":"R2108","pred":"prep","subj":"T7289","obj":"T7260"},{"id":"R2109","pred":"nummod","subj":"T7290","obj":"T7291"},{"id":"R2110","pred":"pobj","subj":"T7291","obj":"T7289"},{"id":"R2111","pred":"prep","subj":"T7292","obj":"T7260"},{"id":"R2112","pred":"npadvmod","subj":"T7293","obj":"T7294"},{"id":"R2113","pred":"amod","subj":"T7294","obj":"T7296"},{"id":"R2114","pred":"punct","subj":"T7295","obj":"T7294"},{"id":"R2115","pred":"pobj","subj":"T7296","obj":"T7292"},{"id":"R2116","pred":"punct","subj":"T7297","obj":"T7298"},{"id":"R2117","pred":"parataxis","subj":"T7298","obj":"T7296"},{"id":"R2118","pred":"punct","subj":"T7299","obj":"T7298"},{"id":"R2119","pred":"prep","subj":"T7300","obj":"T7296"},{"id":"R2120","pred":"nummod","subj":"T7301","obj":"T7302"},{"id":"R2121","pred":"pobj","subj":"T7302","obj":"T7300"},{"id":"R2122","pred":"punct","subj":"T7303","obj":"T7304"},{"id":"R2123","pred":"prep","subj":"T7304","obj":"T7302"},{"id":"R2124","pred":"advcl","subj":"T7305","obj":"T7260"},{"id":"R2125","pred":"agent","subj":"T7306","obj":"T7305"},{"id":"R2126","pred":"nummod","subj":"T7307","obj":"T7308"},{"id":"R2127","pred":"pobj","subj":"T7308","obj":"T7306"},{"id":"R2128","pred":"prep","subj":"T7309","obj":"T7308"},{"id":"R2129","pred":"compound","subj":"T7310","obj":"T7311"},{"id":"R2130","pred":"pobj","subj":"T7311","obj":"T7309"},{"id":"R2131","pred":"cc","subj":"T7312","obj":"T7308"},{"id":"R2132","pred":"nummod","subj":"T7313","obj":"T7314"},{"id":"R2133","pred":"compound","subj":"T7314","obj":"T7315"},{"id":"R2134","pred":"conj","subj":"T7315","obj":"T7308"},{"id":"R2135","pred":"prep","subj":"T7316","obj":"T7315"},{"id":"R2136","pred":"advmod","subj":"T7317","obj":"T7318"},{"id":"R2137","pred":"amod","subj":"T7318","obj":"T7319"},{"id":"R2138","pred":"pobj","subj":"T7319","obj":"T7316"},{"id":"R2139","pred":"amod","subj":"T7320","obj":"T7319"},{"id":"R2140","pred":"punct","subj":"T7321","obj":"T7322"},{"id":"R2141","pred":"parataxis","subj":"T7322","obj":"T7260"},{"id":"R2142","pred":"quantmod","subj":"T7323","obj":"T7324"},{"id":"R2143","pred":"nummod","subj":"T7324","obj":"T7322"},{"id":"R2144","pred":"punct","subj":"T7325","obj":"T7324"},{"id":"R2145","pred":"punct","subj":"T7326","obj":"T7327"},{"id":"R2146","pred":"prep","subj":"T7327","obj":"T7322"},{"id":"R2147","pred":"punct","subj":"T7328","obj":"T7322"},{"id":"R2148","pred":"punct","subj":"T7329","obj":"T7260"}],"attributes":[{"subj":"T7258","pred":"source","obj":"craft-sa-dev"},{"subj":"T7259","pred":"source","obj":"craft-sa-dev"},{"subj":"T7260","pred":"source","obj":"craft-sa-dev"},{"subj":"T7261","pred":"source","obj":"craft-sa-dev"},{"subj":"T7262","pred":"source","obj":"craft-sa-dev"},{"subj":"T7263","pred":"source","obj":"craft-sa-dev"},{"subj":"T7264","pred":"source","obj":"craft-sa-dev"},{"subj":"T7265","pred":"source","obj":"craft-sa-dev"},{"subj":"T7266","pred":"source","obj":"craft-sa-dev"},{"subj":"T7267","pred":"source","obj":"craft-sa-dev"},{"subj":"T7268","pred":"source","obj":"craft-sa-dev"},{"subj":"T7269","pred":"source","obj":"craft-sa-dev"},{"subj":"T7270","pred":"source","obj":"craft-sa-dev"},{"subj":"T7271","pred":"source","obj":"craft-sa-dev"},{"subj":"T7272","pred":"source","obj":"craft-sa-dev"},{"subj":"T7273","pred":"source","obj":"craft-sa-dev"},{"subj":"T7274","pred":"source","obj":"craft-sa-dev"},{"subj":"T7275","pred":"source","obj":"craft-sa-dev"},{"subj":"T7276","pred":"source","obj":"craft-sa-dev"},{"subj":"T7277","pred":"source","obj":"craft-sa-dev"},{"subj":"T7278","pred":"source","obj":"craft-sa-dev"},{"subj":"T7279","pred":"source","obj":"craft-sa-dev"},{"subj":"T7280","pred":"source","obj":"craft-sa-dev"},{"subj":"T7281","pred":"source","obj":"craft-sa-dev"},{"subj":"T7282","pred":"source","obj":"craft-sa-dev"},{"subj":"T7283","pred":"source","obj":"craft-sa-dev"},{"subj":"T7284","pred":"source","obj":"craft-sa-dev"},{"subj":"T7285","pred":"source","obj":"craft-sa-dev"},{"subj":"T7286","pred":"source","obj":"craft-sa-dev"},{"subj":"T7287","pred":"source","obj":"craft-sa-dev"},{"subj":"T7288","pred":"source","obj":"craft-sa-dev"},{"subj":"T7289","pred":"source","obj":"craft-sa-dev"},{"subj":"T7290","pred":"source","obj":"craft-sa-dev"},{"subj":"T7291","pred":"source","obj":"craft-sa-dev"},{"subj":"T7292","pred":"source","obj":"craft-sa-dev"},{"subj":"T7293","pred":"source","obj":"craft-sa-dev"},{"subj":"T7294","pred":"source","obj":"craft-sa-dev"},{"subj":"T7295","pred":"source","obj":"craft-sa-dev"},{"subj":"T7296","pred":"source","obj":"craft-sa-dev"},{"subj":"T7297","pred":"source","obj":"craft-sa-dev"},{"subj":"T7298","pred":"source","obj":"craft-sa-dev"},{"subj":"T7299","pred":"source","obj":"craft-sa-dev"},{"subj":"T7300","pred":"source","obj":"craft-sa-dev"},{"subj":"T7301","pred":"source","obj":"craft-sa-dev"},{"subj":"T7302","pred":"source","obj":"craft-sa-dev"},{"subj":"T7303","pred":"source","obj":"craft-sa-dev"},{"subj":"T7304","pred":"source","obj":"craft-sa-dev"},{"subj":"T7305","pred":"source","obj":"craft-sa-dev"},{"subj":"T7306","pred":"source","obj":"craft-sa-dev"},{"subj":"T7307","pred":"source","obj":"craft-sa-dev"},{"subj":"T7308","pred":"source","obj":"craft-sa-dev"},{"subj":"T7309","pred":"source","obj":"craft-sa-dev"},{"subj":"T7310","pred":"source","obj":"craft-sa-dev"},{"subj":"T7311","pred":"source","obj":"craft-sa-dev"},{"subj":"T7312","pred":"source","obj":"craft-sa-dev"},{"subj":"T7313","pred":"source","obj":"craft-sa-dev"},{"subj":"T7314","pred":"source","obj":"craft-sa-dev"},{"subj":"T7315","pred":"source","obj":"craft-sa-dev"},{"subj":"T7316","pred":"source","obj":"craft-sa-dev"},{"subj":"T7317","pred":"source","obj":"craft-sa-dev"},{"subj":"T7318","pred":"source","obj":"craft-sa-dev"},{"subj":"T7319","pred":"source","obj":"craft-sa-dev"},{"subj":"T7320","pred":"source","obj":"craft-sa-dev"},{"subj":"T7321","pred":"source","obj":"craft-sa-dev"},{"subj":"T7322","pred":"source","obj":"craft-sa-dev"},{"subj":"T7323","pred":"source","obj":"craft-sa-dev"},{"subj":"T7324","pred":"source","obj":"craft-sa-dev"},{"subj":"T7325","pred":"source","obj":"craft-sa-dev"},{"subj":"T7326","pred":"source","obj":"craft-sa-dev"},{"subj":"T7327","pred":"source","obj":"craft-sa-dev"},{"subj":"T7328","pred":"source","obj":"craft-sa-dev"},{"subj":"T7329","pred":"source","obj":"craft-sa-dev"}]},{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T7164","span":{"begin":45,"end":49},"obj":"NCBITaxon:9925"},{"id":"T7165","span":{"begin":50,"end":53},"obj":"GO:0071735"},{"id":"T7166","span":{"begin":107,"end":113},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T7167","span":{"begin":117,"end":122},"obj":"CL_GO_EXT:cell"},{"id":"T7168","span":{"begin":154,"end":156},"obj":"PR_EXT:000006300"},{"id":"T7169","span":{"begin":170,"end":175},"obj":"UBERON:0001977"},{"id":"T7170","span":{"begin":220,"end":226},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T7171","span":{"begin":271,"end":278},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T7172","span":{"begin":286,"end":294},"obj":"GO:0042571"}],"attributes":[{"subj":"T7164","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T7165","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T7166","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T7167","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T7168","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T7169","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T7170","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T7171","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T7172","pred":"source","obj":"craft-ca-core-ex-dev"}]},{"project":"craft-ca-core-dev","denotations":[{"id":"T7147","span":{"begin":45,"end":49},"obj":"NCBITaxon:9925"},{"id":"T7148","span":{"begin":50,"end":53},"obj":"GO:0071735"},{"id":"T7149","span":{"begin":154,"end":156},"obj":"PR:000006300"},{"id":"T7150","span":{"begin":170,"end":175},"obj":"UBERON:0001977"},{"id":"T7151","span":{"begin":286,"end":294},"obj":"GO:0042571"}],"attributes":[{"subj":"T7147","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T7148","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T7149","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T7150","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T7151","pred":"source","obj":"craft-ca-core-dev"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"craft-sa-dev","color":"#ece493","default":true},{"id":"craft-ca-core-ex-dev","color":"#ca93ec"},{"id":"craft-ca-core-dev","color":"#93ecb0"}]}]}}