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PMC:1448208 / 12941-13248 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T7258 0-307 sentence denotes Following a 30 min incubation with purified goat IgG (50 (μg/ml) at 25°C and three additional washes with Buffer A, cells were incubated for 60 min with E2-specific antiserum [30] at 1 μg/ml followed by three washes in Buffer A and 60 minute incubation in fluorescently labeled second antibody (1–2 μg/ml).
T7259 1-10 VBG denotes Following
T7260 128-137 VBN denotes incubated
T7261 11-12 DT denotes a
T7262 20-30 NN denotes incubation
T7263 13-15 CD denotes 30
T7264 16-19 NN denotes min
T7265 31-35 IN denotes with
T7266 36-44 VBN denotes purified
T7267 50-53 NN denotes IgG
T7268 45-49 NN denotes goat
T7269 54-55 -LRB- denotes (
T7270 59-61 NN denotes μg
T7271 55-57 CD denotes 50
T7272 58-59 -LRB- denotes (
T7273 61-62 SYM denotes /
T7274 62-64 NN denotes ml
T7275 64-65 -RRB- denotes )
T7276 66-68 IN denotes at
T7277 69-71 CD denotes 25
T7278 71-73 NN denotes °C
T7279 74-77 CC denotes and
T7280 78-83 CD denotes three
T7281 95-101 NNS denotes washes
T7282 84-94 JJ denotes additional
T7283 102-106 IN denotes with
T7284 107-113 NN denotes Buffer
T7285 114-115 NN denotes A
T7286 115-117 , denotes ,
T7287 117-122 NNS denotes cells
T7288 123-127 VBD denotes were
T7289 138-141 IN denotes for
T7290 142-144 CD denotes 60
T7291 145-148 NN denotes min
T7292 149-153 IN denotes with
T7293 154-156 NN denotes E2
T7294 157-165 JJ denotes specific
T7295 156-157 HYPH denotes -
T7296 166-175 NN denotes antiserum
T7297 176-177 -LRB- denotes [
T7298 177-179 CD denotes 30
T7299 179-180 -RRB- denotes ]
T7300 181-183 IN denotes at
T7301 184-185 CD denotes 1
T7302 186-188 NN denotes μg
T7303 188-189 SYM denotes /
T7304 189-191 NN denotes ml
T7305 192-200 VBN denotes followed
T7306 201-203 IN denotes by
T7307 204-209 CD denotes three
T7308 210-216 NNS denotes washes
T7309 217-219 IN denotes in
T7310 220-226 NN denotes Buffer
T7311 227-228 NN denotes A
T7312 229-232 CC denotes and
T7313 233-235 CD denotes 60
T7314 236-242 NN denotes minute
T7315 243-253 NN denotes incubation
T7316 254-256 IN denotes in
T7317 257-270 RB denotes fluorescently
T7318 271-278 VBN denotes labeled
T7319 286-294 NN denotes antibody
T7320 279-285 JJ denotes second
T7321 295-296 -LRB- denotes (
T7322 300-302 NN denotes μg
T7323 296-297 CD denotes 1
T7324 298-299 CD denotes 2
T7325 297-298 SYM denotes –
T7326 302-303 SYM denotes /
T7327 303-305 NN denotes ml
T7328 305-306 -RRB- denotes )
T7329 306-307 . denotes .
R2079 T7259 T7260 prep Following,incubated
R2080 T7261 T7262 det a,incubation
R2081 T7262 T7259 pobj incubation,Following
R2082 T7263 T7264 nummod 30,min
R2083 T7264 T7262 compound min,incubation
R2084 T7265 T7262 prep with,incubation
R2085 T7266 T7267 amod purified,IgG
R2086 T7267 T7265 pobj IgG,with
R2087 T7268 T7267 compound goat,IgG
R2088 T7269 T7270 punct (,μg
R2089 T7270 T7267 parataxis μg,IgG
R2090 T7271 T7270 nummod 50,μg
R2091 T7272 T7270 punct (,μg
R2092 T7273 T7274 punct /,ml
R2093 T7274 T7270 prep ml,μg
R2094 T7275 T7270 punct ),μg
R2095 T7276 T7262 prep at,incubation
R2096 T7277 T7278 nummod 25,°C
R2097 T7278 T7276 pobj °C,at
R2098 T7279 T7262 cc and,incubation
R2099 T7280 T7281 nummod three,washes
R2100 T7281 T7262 conj washes,incubation
R2101 T7282 T7281 amod additional,washes
R2102 T7283 T7281 prep with,washes
R2103 T7284 T7285 compound Buffer,A
R2104 T7285 T7283 pobj A,with
R2105 T7286 T7260 punct ", ",incubated
R2106 T7287 T7260 nsubjpass cells,incubated
R2107 T7288 T7260 auxpass were,incubated
R2108 T7289 T7260 prep for,incubated
R2109 T7290 T7291 nummod 60,min
R2110 T7291 T7289 pobj min,for
R2111 T7292 T7260 prep with,incubated
R2112 T7293 T7294 npadvmod E2,specific
R2113 T7294 T7296 amod specific,antiserum
R2114 T7295 T7294 punct -,specific
R2115 T7296 T7292 pobj antiserum,with
R2116 T7297 T7298 punct [,30
R2117 T7298 T7296 parataxis 30,antiserum
R2118 T7299 T7298 punct ],30
R2119 T7300 T7296 prep at,antiserum
R2120 T7301 T7302 nummod 1,μg
R2121 T7302 T7300 pobj μg,at
R2122 T7303 T7304 punct /,ml
R2123 T7304 T7302 prep ml,μg
R2124 T7305 T7260 advcl followed,incubated
R2125 T7306 T7305 agent by,followed
R2126 T7307 T7308 nummod three,washes
R2127 T7308 T7306 pobj washes,by
R2128 T7309 T7308 prep in,washes
R2129 T7310 T7311 compound Buffer,A
R2130 T7311 T7309 pobj A,in
R2131 T7312 T7308 cc and,washes
R2132 T7313 T7314 nummod 60,minute
R2133 T7314 T7315 compound minute,incubation
R2134 T7315 T7308 conj incubation,washes
R2135 T7316 T7315 prep in,incubation
R2136 T7317 T7318 advmod fluorescently,labeled
R2137 T7318 T7319 amod labeled,antibody
R2138 T7319 T7316 pobj antibody,in
R2139 T7320 T7319 amod second,antibody
R2140 T7321 T7322 punct (,μg
R2141 T7322 T7260 parataxis μg,incubated
R2142 T7323 T7324 quantmod 1,2
R2143 T7324 T7322 nummod 2,μg
R2144 T7325 T7324 punct –,2
R2145 T7326 T7327 punct /,ml
R2146 T7327 T7322 prep ml,μg
R2147 T7328 T7322 punct ),μg
R2148 T7329 T7260 punct .,incubated

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7164 45-49 NCBITaxon:9925 denotes goat
T7165 50-53 GO:0071735 denotes IgG
T7166 107-113 CHEBI_CHMO_EXT:buffer_solution denotes Buffer
T7167 117-122 CL_GO_EXT:cell denotes cells
T7168 154-156 PR_EXT:000006300 denotes E2
T7169 170-175 UBERON:0001977 denotes serum
T7170 220-226 CHEBI_CHMO_EXT:buffer_solution denotes Buffer
T7171 271-278 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T7172 286-294 GO:0042571 denotes antibody

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7147 45-49 NCBITaxon:9925 denotes goat
T7148 50-53 GO:0071735 denotes IgG
T7149 154-156 PR:000006300 denotes E2
T7150 170-175 UBERON:0001977 denotes serum
T7151 286-294 GO:0042571 denotes antibody