PMC:1448208 / 11495-12295 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T6426","span":{"begin":0,"end":10},"obj":"NN"},{"id":"T6427","span":{"begin":11,"end":13},"obj":"IN"},{"id":"T6428","span":{"begin":14,"end":26},"obj":"JJ"},{"id":"T6429","span":{"begin":39,"end":44},"obj":"NN"},{"id":"T6430","span":{"begin":27,"end":31},"obj":"NN"},{"id":"T6431","span":{"begin":32,"end":38},"obj":"JJ"},{"id":"T6432","span":{"begin":44,"end":301},"obj":"sentence"},{"id":"T6433","span":{"begin":45,"end":48},"obj":"DT"},{"id":"T6434","span":{"begin":75,"end":80},"obj":"NNS"},{"id":"T6435","span":{"begin":49,"end":56},"obj":"JJ"},{"id":"T6436","span":{"begin":57,"end":60},"obj":"NN"},{"id":"T6437","span":{"begin":61,"end":63},"obj":"NN"},{"id":"T6438","span":{"begin":60,"end":61},"obj":"HYPH"},{"id":"T6439","span":{"begin":64,"end":74},"obj":"JJ"},{"id":"T6440","span":{"begin":95,"end":100},"obj":"VBN"},{"id":"T6441","span":{"begin":81,"end":89},"obj":"VBN"},{"id":"T6442","span":{"begin":90,"end":94},"obj":"VBD"},{"id":"T6443","span":{"begin":101,"end":114},"obj":"RB"},{"id":"T6444","span":{"begin":115,"end":117},"obj":"IN"},{"id":"T6445","span":{"begin":118,"end":122},"obj":"NNS"},{"id":"T6446","span":{"begin":123,"end":127},"obj":"WDT"},{"id":"T6447","span":{"begin":128,"end":132},"obj":"VBD"},{"id":"T6448","span":{"begin":133,"end":145},"obj":"JJ"},{"id":"T6449","span":{"begin":146,"end":149},"obj":"IN"},{"id":"T6450","span":{"begin":150,"end":154},"obj":"CC"},{"id":"T6451","span":{"begin":162,"end":170},"obj":"NN"},{"id":"T6452","span":{"begin":155,"end":158},"obj":"DT"},{"id":"T6453","span":{"begin":159,"end":161},"obj":"NN"},{"id":"T6454","span":{"begin":171,"end":174},"obj":"CC"},{"id":"T6455","span":{"begin":175,"end":178},"obj":"DT"},{"id":"T6456","span":{"begin":187,"end":196},"obj":"NN"},{"id":"T6457","span":{"begin":179,"end":182},"obj":"NN"},{"id":"T6458","span":{"begin":183,"end":186},"obj":"NN"},{"id":"T6459","span":{"begin":182,"end":183},"obj":"HYPH"},{"id":"T6460","span":{"begin":197,"end":198},"obj":"-LRB-"},{"id":"T6461","span":{"begin":289,"end":299},"obj":"NN"},{"id":"T6462","span":{"begin":198,"end":200},"obj":"NN"},{"id":"T6463","span":{"begin":215,"end":220},"obj":"NN"},{"id":"T6464","span":{"begin":200,"end":201},"obj":"-LRB-"},{"id":"T6465","span":{"begin":201,"end":207},"obj":"NN"},{"id":"T6466","span":{"begin":207,"end":208},"obj":"-RRB-"},{"id":"T6467","span":{"begin":208,"end":211},"obj":"NN"},{"id":"T6468","span":{"begin":212,"end":213},"obj":"NN"},{"id":"T6469","span":{"begin":211,"end":212},"obj":"HYPH"},{"id":"T6470","span":{"begin":213,"end":214},"obj":":"},{"id":"T6471","span":{"begin":221,"end":225},"obj":"CD"},{"id":"T6472","span":{"begin":225,"end":226},"obj":":"},{"id":"T6473","span":{"begin":227,"end":230},"obj":"DT"},{"id":"T6474","span":{"begin":239,"end":249},"obj":"NNP"},{"id":"T6475","span":{"begin":231,"end":238},"obj":"NNP"},{"id":"T6476","span":{"begin":249,"end":251},"obj":","},{"id":"T6477","span":{"begin":251,"end":254},"obj":"NNP"},{"id":"T6478","span":{"begin":255,"end":261},"obj":"NNP"},{"id":"T6479","span":{"begin":261,"end":263},"obj":","},{"id":"T6480","span":{"begin":263,"end":265},"obj":"NNP"},{"id":"T6481","span":{"begin":265,"end":266},"obj":":"},{"id":"T6482","span":{"begin":267,"end":271},"obj":"NN"},{"id":"T6483","span":{"begin":272,"end":273},"obj":"SYM"},{"id":"T6484","span":{"begin":274,"end":277},"obj":"NN"},{"id":"T6485","span":{"begin":278,"end":279},"obj":"SYM"},{"id":"T6486","span":{"begin":280,"end":285},"obj":"NN"},{"id":"T6487","span":{"begin":286,"end":288},"obj":"NN"},{"id":"T6488","span":{"begin":285,"end":286},"obj":"HYPH"},{"id":"T6489","span":{"begin":299,"end":300},"obj":"-RRB-"},{"id":"T6490","span":{"begin":300,"end":301},"obj":"."},{"id":"T6491","span":{"begin":301,"end":602},"obj":"sentence"},{"id":"T6492","span":{"begin":302,"end":315},"obj":"NN"},{"id":"T6493","span":{"begin":387,"end":395},"obj":"VBD"},{"id":"T6494","span":{"begin":316,"end":318},"obj":"IN"},{"id":"T6495","span":{"begin":319,"end":326},"obj":"NNS"},{"id":"T6496","span":{"begin":327,"end":331},"obj":"WDT"},{"id":"T6497","span":{"begin":332,"end":336},"obj":"VBD"},{"id":"T6498","span":{"begin":337,"end":349},"obj":"JJ"},{"id":"T6499","span":{"begin":350,"end":353},"obj":"IN"},{"id":"T6500","span":{"begin":354,"end":358},"obj":"DT"},{"id":"T6501","span":{"begin":359,"end":369},"obj":"NNS"},{"id":"T6502","span":{"begin":370,"end":373},"obj":"CC"},{"id":"T6503","span":{"begin":374,"end":377},"obj":"DT"},{"id":"T6504","span":{"begin":378,"end":386},"obj":"NN"},{"id":"T6505","span":{"begin":396,"end":398},"obj":"IN"},{"id":"T6506","span":{"begin":399,"end":402},"obj":"DT"},{"id":"T6507","span":{"begin":403,"end":413},"obj":"NN"},{"id":"T6508","span":{"begin":414,"end":416},"obj":"IN"},{"id":"T6509","span":{"begin":417,"end":421},"obj":"NNS"},{"id":"T6510","span":{"begin":422,"end":426},"obj":"IN"},{"id":"T6511","span":{"begin":427,"end":428},"obj":"DT"},{"id":"T6512","span":{"begin":429,"end":436},"obj":"NN"},{"id":"T6513","span":{"begin":437,"end":439},"obj":"IN"},{"id":"T6514","span":{"begin":440,"end":449},"obj":"NNS"},{"id":"T6515","span":{"begin":450,"end":459},"obj":"VBG"},{"id":"T6516","span":{"begin":460,"end":464},"obj":"DT"},{"id":"T6517","span":{"begin":465,"end":472},"obj":"NNS"},{"id":"T6518","span":{"begin":473,"end":477},"obj":"WDT"},{"id":"T6519","span":{"begin":478,"end":482},"obj":"VBD"},{"id":"T6520","span":{"begin":483,"end":493},"obj":"JJ"},{"id":"T6521","span":{"begin":494,"end":497},"obj":"IN"},{"id":"T6522","span":{"begin":498,"end":501},"obj":"DT"},{"id":"T6523","span":{"begin":502,"end":510},"obj":"NN"},{"id":"T6524","span":{"begin":511,"end":514},"obj":"CC"},{"id":"T6525","span":{"begin":515,"end":519},"obj":"VBD"},{"id":"T6526","span":{"begin":520,"end":528},"obj":"JJ"},{"id":"T6527","span":{"begin":529,"end":532},"obj":"IN"},{"id":"T6528","span":{"begin":533,"end":537},"obj":"DT"},{"id":"T6529","span":{"begin":538,"end":548},"obj":"NNS"},{"id":"T6530","span":{"begin":549,"end":550},"obj":"-LRB-"},{"id":"T6531","span":{"begin":553,"end":558},"obj":"VBP"},{"id":"T6532","span":{"begin":550,"end":552},"obj":"PRP"},{"id":"T6533","span":{"begin":559,"end":561},"obj":"IN"},{"id":"T6534","span":{"begin":562,"end":566},"obj":"DT"},{"id":"T6535","span":{"begin":567,"end":575},"obj":"NN"},{"id":"T6536","span":{"begin":576,"end":578},"obj":"IN"},{"id":"T6537","span":{"begin":579,"end":582},"obj":"DT"},{"id":"T6538","span":{"begin":592,"end":600},"obj":"NN"},{"id":"T6539","span":{"begin":583,"end":584},"obj":"``"},{"id":"T6540","span":{"begin":584,"end":590},"obj":"NN"},{"id":"T6541","span":{"begin":590,"end":591},"obj":"''"},{"id":"T6542","span":{"begin":600,"end":601},"obj":"-RRB-"},{"id":"T6543","span":{"begin":601,"end":602},"obj":"."},{"id":"T6544","span":{"begin":602,"end":800},"obj":"sentence"},{"id":"T6545","span":{"begin":603,"end":605},"obj":"IN"},{"id":"T6546","span":{"begin":678,"end":682},"obj":"VBD"},{"id":"T6547","span":{"begin":606,"end":609},"obj":"PRP$"},{"id":"T6548","span":{"begin":610,"end":618},"obj":"NN"},{"id":"T6549","span":{"begin":619,"end":622},"obj":"IN"},{"id":"T6550","span":{"begin":623,"end":631},"obj":"VBG"},{"id":"T6551","span":{"begin":632,"end":635},"obj":"DT"},{"id":"T6552","span":{"begin":652,"end":661},"obj":"NN"},{"id":"T6553","span":{"begin":636,"end":644},"obj":"JJ"},{"id":"T6554","span":{"begin":645,"end":651},"obj":"JJ"},{"id":"T6555","span":{"begin":662,"end":664},"obj":"IN"},{"id":"T6556","span":{"begin":665,"end":668},"obj":"DT"},{"id":"T6557","span":{"begin":669,"end":677},"obj":"NN"},{"id":"T6558","span":{"begin":765,"end":772},"obj":"VB"},{"id":"T6559","span":{"begin":683,"end":693},"obj":"JJ"},{"id":"T6560","span":{"begin":693,"end":695},"obj":","},{"id":"T6561","span":{"begin":695,"end":699},"obj":"RB"},{"id":"T6562","span":{"begin":700,"end":705},"obj":"DT"},{"id":"T6563","span":{"begin":726,"end":733},"obj":"NNS"},{"id":"T6564","span":{"begin":706,"end":716},"obj":"JJ"},{"id":"T6565","span":{"begin":717,"end":725},"obj":"NN"},{"id":"T6566","span":{"begin":734,"end":738},"obj":"WDT"},{"id":"T6567","span":{"begin":739,"end":742},"obj":"VBD"},{"id":"T6568","span":{"begin":743,"end":747},"obj":"DT"},{"id":"T6569","span":{"begin":748,"end":758},"obj":"NNS"},{"id":"T6570","span":{"begin":759,"end":764},"obj":"MD"},{"id":"T6571","span":{"begin":773,"end":779},"obj":"IN"},{"id":"T6572","span":{"begin":780,"end":783},"obj":"DT"},{"id":"T6573","span":{"begin":793,"end":799},"obj":"NN"},{"id":"T6574","span":{"begin":784,"end":792},"obj":"JJ"},{"id":"T6575","span":{"begin":799,"end":800},"obj":"."}],"relations":[{"id":"R1828","pred":"prep","subj":"T6427","obj":"T6426"},{"id":"R1829","pred":"amod","subj":"T6428","obj":"T6429"},{"id":"R1830","pred":"pobj","subj":"T6429","obj":"T6427"},{"id":"R1831","pred":"nmod","subj":"T6430","obj":"T6429"},{"id":"R1832","pred":"amod","subj":"T6431","obj":"T6429"},{"id":"R1833","pred":"det","subj":"T6433","obj":"T6434"},{"id":"R1834","pred":"nsubjpass","subj":"T6434","obj":"T6440"},{"id":"R1835","pred":"amod","subj":"T6435","obj":"T6434"},{"id":"R1836","pred":"nmod","subj":"T6436","obj":"T6437"},{"id":"R1837","pred":"nmod","subj":"T6437","obj":"T6434"},{"id":"R1838","pred":"punct","subj":"T6438","obj":"T6437"},{"id":"R1839","pred":"amod","subj":"T6439","obj":"T6434"},{"id":"R1840","pred":"acl","subj":"T6441","obj":"T6434"},{"id":"R1841","pred":"auxpass","subj":"T6442","obj":"T6440"},{"id":"R1842","pred":"advmod","subj":"T6443","obj":"T6440"},{"id":"R1843","pred":"prep","subj":"T6444","obj":"T6440"},{"id":"R1844","pred":"pobj","subj":"T6445","obj":"T6444"},{"id":"R1845","pred":"dep","subj":"T6446","obj":"T6447"},{"id":"R1846","pred":"relcl","subj":"T6447","obj":"T6445"},{"id":"R1847","pred":"acomp","subj":"T6448","obj":"T6447"},{"id":"R1848","pred":"prep","subj":"T6449","obj":"T6448"},{"id":"R1849","pred":"preconj","subj":"T6450","obj":"T6451"},{"id":"R1850","pred":"pobj","subj":"T6451","obj":"T6449"},{"id":"R1851","pred":"det","subj":"T6452","obj":"T6451"},{"id":"R1852","pred":"compound","subj":"T6453","obj":"T6451"},{"id":"R1853","pred":"cc","subj":"T6454","obj":"T6451"},{"id":"R1854","pred":"det","subj":"T6455","obj":"T6456"},{"id":"R1855","pred":"conj","subj":"T6456","obj":"T6451"},{"id":"R1856","pred":"compound","subj":"T6457","obj":"T6458"},{"id":"R1857","pred":"compound","subj":"T6458","obj":"T6456"},{"id":"R1858","pred":"punct","subj":"T6459","obj":"T6458"},{"id":"R1859","pred":"punct","subj":"T6460","obj":"T6461"},{"id":"R1860","pred":"parataxis","subj":"T6461","obj":"T6440"},{"id":"R1861","pred":"nmod","subj":"T6462","obj":"T6463"},{"id":"R1862","pred":"dep","subj":"T6463","obj":"T6461"},{"id":"R1863","pred":"punct","subj":"T6464","obj":"T6465"},{"id":"R1864","pred":"parataxis","subj":"T6465","obj":"T6463"},{"id":"R1865","pred":"punct","subj":"T6466","obj":"T6465"},{"id":"R1866","pred":"nmod","subj":"T6467","obj":"T6468"},{"id":"R1867","pred":"nmod","subj":"T6468","obj":"T6463"},{"id":"R1868","pred":"punct","subj":"T6469","obj":"T6468"},{"id":"R1869","pred":"punct","subj":"T6470","obj":"T6463"},{"id":"R1870","pred":"nummod","subj":"T6471","obj":"T6463"},{"id":"R1871","pred":"punct","subj":"T6472","obj":"T6461"},{"id":"R1872","pred":"det","subj":"T6473","obj":"T6474"},{"id":"R1873","pred":"dep","subj":"T6474","obj":"T6461"},{"id":"R1874","pred":"compound","subj":"T6475","obj":"T6474"},{"id":"R1875","pred":"punct","subj":"T6476","obj":"T6474"},{"id":"R1876","pred":"compound","subj":"T6477","obj":"T6478"},{"id":"R1877","pred":"npadvmod","subj":"T6478","obj":"T6474"},{"id":"R1878","pred":"punct","subj":"T6479","obj":"T6474"},{"id":"R1879","pred":"npadvmod","subj":"T6480","obj":"T6474"},{"id":"R1880","pred":"punct","subj":"T6481","obj":"T6461"},{"id":"R1881","pred":"nmod","subj":"T6482","obj":"T6461"},{"id":"R1882","pred":"punct","subj":"T6483","obj":"T6482"},{"id":"R1883","pred":"appos","subj":"T6484","obj":"T6482"},{"id":"R1884","pred":"punct","subj":"T6485","obj":"T6482"},{"id":"R1885","pred":"compound","subj":"T6486","obj":"T6487"},{"id":"R1886","pred":"appos","subj":"T6487","obj":"T6482"},{"id":"R1887","pred":"punct","subj":"T6488","obj":"T6487"},{"id":"R1888","pred":"punct","subj":"T6489","obj":"T6461"},{"id":"R1889","pred":"punct","subj":"T6490","obj":"T6440"},{"id":"R1890","pred":"nsubj","subj":"T6492","obj":"T6493"},{"id":"R1891","pred":"prep","subj":"T6494","obj":"T6492"},{"id":"R1892","pred":"pobj","subj":"T6495","obj":"T6494"},{"id":"R1893","pred":"dep","subj":"T6496","obj":"T6497"},{"id":"R1894","pred":"relcl","subj":"T6497","obj":"T6495"},{"id":"R1895","pred":"acomp","subj":"T6498","obj":"T6497"},{"id":"R1896","pred":"prep","subj":"T6499","obj":"T6498"},{"id":"R1897","pred":"det","subj":"T6500","obj":"T6501"},{"id":"R1898","pred":"pobj","subj":"T6501","obj":"T6499"},{"id":"R1899","pred":"cc","subj":"T6502","obj":"T6501"},{"id":"R1900","pred":"det","subj":"T6503","obj":"T6504"},{"id":"R1901","pred":"conj","subj":"T6504","obj":"T6501"},{"id":"R1902","pred":"prep","subj":"T6505","obj":"T6493"},{"id":"R1903","pred":"det","subj":"T6506","obj":"T6507"},{"id":"R1904","pred":"pobj","subj":"T6507","obj":"T6505"},{"id":"R1905","pred":"prep","subj":"T6508","obj":"T6507"},{"id":"R1906","pred":"pobj","subj":"T6509","obj":"T6508"},{"id":"R1907","pred":"prep","subj":"T6510","obj":"T6509"},{"id":"R1908","pred":"det","subj":"T6511","obj":"T6512"},{"id":"R1909","pred":"pobj","subj":"T6512","obj":"T6510"},{"id":"R1910","pred":"prep","subj":"T6513","obj":"T6512"},{"id":"R1911","pred":"pobj","subj":"T6514","obj":"T6513"},{"id":"R1912","pred":"prep","subj":"T6515","obj":"T6509"},{"id":"R1913","pred":"det","subj":"T6516","obj":"T6517"},{"id":"R1914","pred":"pobj","subj":"T6517","obj":"T6515"},{"id":"R1915","pred":"dep","subj":"T6518","obj":"T6519"},{"id":"R1916","pred":"relcl","subj":"T6519","obj":"T6517"},{"id":"R1917","pred":"acomp","subj":"T6520","obj":"T6519"},{"id":"R1918","pred":"prep","subj":"T6521","obj":"T6520"},{"id":"R1919","pred":"det","subj":"T6522","obj":"T6523"},{"id":"R1920","pred":"pobj","subj":"T6523","obj":"T6521"},{"id":"R1921","pred":"cc","subj":"T6524","obj":"T6519"},{"id":"R1922","pred":"conj","subj":"T6525","obj":"T6519"},{"id":"R1923","pred":"acomp","subj":"T6526","obj":"T6525"},{"id":"R1924","pred":"prep","subj":"T6527","obj":"T6526"},{"id":"R1925","pred":"det","subj":"T6528","obj":"T6529"},{"id":"R1926","pred":"pobj","subj":"T6529","obj":"T6527"},{"id":"R1927","pred":"punct","subj":"T6530","obj":"T6531"},{"id":"R1928","pred":"parataxis","subj":"T6531","obj":"T6525"},{"id":"R1929","pred":"nsubj","subj":"T6532","obj":"T6531"},{"id":"R1930","pred":"prep","subj":"T6533","obj":"T6531"},{"id":"R1931","pred":"det","subj":"T6534","obj":"T6535"},{"id":"R1932","pred":"pobj","subj":"T6535","obj":"T6533"},{"id":"R1933","pred":"prep","subj":"T6536","obj":"T6531"},{"id":"R1934","pred":"det","subj":"T6537","obj":"T6538"},{"id":"R1935","pred":"pobj","subj":"T6538","obj":"T6536"},{"id":"R1936","pred":"punct","subj":"T6539","obj":"T6538"},{"id":"R1937","pred":"nmod","subj":"T6540","obj":"T6538"},{"id":"R1938","pred":"punct","subj":"T6541","obj":"T6538"},{"id":"R1939","pred":"punct","subj":"T6542","obj":"T6531"},{"id":"R1940","pred":"punct","subj":"T6543","obj":"T6493"},{"id":"R1941","pred":"mark","subj":"T6545","obj":"T6546"},{"id":"R1942","pred":"advcl","subj":"T6546","obj":"T6558"},{"id":"R1943","pred":"poss","subj":"T6547","obj":"T6548"},{"id":"R1944","pred":"nsubj","subj":"T6548","obj":"T6546"},{"id":"R1945","pred":"prep","subj":"T6549","obj":"T6548"},{"id":"R1946","pred":"pcomp","subj":"T6550","obj":"T6549"},{"id":"R1947","pred":"det","subj":"T6551","obj":"T6552"},{"id":"R1948","pred":"dobj","subj":"T6552","obj":"T6550"},{"id":"R1949","pred":"amod","subj":"T6553","obj":"T6552"},{"id":"R1950","pred":"amod","subj":"T6554","obj":"T6552"},{"id":"R1951","pred":"prep","subj":"T6555","obj":"T6552"},{"id":"R1952","pred":"det","subj":"T6556","obj":"T6557"},{"id":"R1953","pred":"pobj","subj":"T6557","obj":"T6555"},{"id":"R1954","pred":"acomp","subj":"T6559","obj":"T6546"},{"id":"R1955","pred":"punct","subj":"T6560","obj":"T6558"},{"id":"R1956","pred":"advmod","subj":"T6561","obj":"T6558"},{"id":"R1957","pred":"det","subj":"T6562","obj":"T6563"},{"id":"R1958","pred":"nsubj","subj":"T6563","obj":"T6558"},{"id":"R1959","pred":"amod","subj":"T6564","obj":"T6563"},{"id":"R1960","pred":"compound","subj":"T6565","obj":"T6563"},{"id":"R1961","pred":"dep","subj":"T6566","obj":"T6567"},{"id":"R1962","pred":"relcl","subj":"T6567","obj":"T6563"},{"id":"R1963","pred":"det","subj":"T6568","obj":"T6569"},{"id":"R1964","pred":"dobj","subj":"T6569","obj":"T6567"},{"id":"R1965","pred":"aux","subj":"T6570","obj":"T6558"},{"id":"R1966","pred":"prep","subj":"T6571","obj":"T6558"},{"id":"R1967","pred":"det","subj":"T6572","obj":"T6573"},{"id":"R1968","pred":"pobj","subj":"T6573","obj":"T6571"},{"id":"R1969","pred":"amod","subj":"T6574","obj":"T6573"},{"id":"R1970","pred":"punct","subj":"T6575","obj":"T6558"}],"text":"Production of intermediate MSUD murine model\nThe various TRE-E2 transgenic lines produced were mated independently to mice that were heterozygous for both the E2 knockout and the LAP-tTA transgene [Tg(tTALap)Bjd/J; Stock 3272; The Jackson Laboratory, Bar Harbor, ME; NMRI × FVB × C57BL/6J background]. Interbreeding of animals that were heterozygous for both transgenes and the knockout resulted in the production of mice with a variety of genotypes including some animals that were homozygous for the knockout and were positive for both transgenes (we refer to this genotype as the \"rescue\" genotype). If our strategy for rescuing the neonatal lethal phenotype of the knockout were successful, then those homozygous knockout animals that had both transgenes would survive beyond the neonatal period."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T6401","span":{"begin":32,"end":38},"obj":"NCBITaxon:39107"},{"id":"T6402","span":{"begin":61,"end":63},"obj":"PR_EXT:000006300"},{"id":"T6403","span":{"begin":64,"end":74},"obj":"SO_EXT:transgenic_entity"},{"id":"T6404","span":{"begin":95,"end":100},"obj":"GO:0007618"},{"id":"T6405","span":{"begin":118,"end":122},"obj":"NCBITaxon:10088"},{"id":"T6406","span":{"begin":159,"end":161},"obj":"PR_EXT:000006300"},{"id":"T6407","span":{"begin":179,"end":182},"obj":"PR_EXT:000005308"},{"id":"T6408","span":{"begin":187,"end":196},"obj":"SO_EXT:0000902"},{"id":"T6409","span":{"begin":198,"end":200},"obj":"SO_EXT:transgenic_entity"},{"id":"T6410","span":{"begin":307,"end":315},"obj":"GO_EXT:breeding"},{"id":"T6411","span":{"begin":319,"end":326},"obj":"NCBITaxon:33208"},{"id":"T6412","span":{"begin":359,"end":369},"obj":"SO_EXT:0000902"},{"id":"T6413","span":{"begin":417,"end":421},"obj":"NCBITaxon:10088"},{"id":"T6414","span":{"begin":440,"end":449},"obj":"SO_EXT:genotype_or_entity_with_genotype"},{"id":"T6415","span":{"begin":465,"end":472},"obj":"NCBITaxon:33208"},{"id":"T6416","span":{"begin":538,"end":548},"obj":"SO_EXT:0000902"},{"id":"T6417","span":{"begin":567,"end":575},"obj":"SO_EXT:genotype_or_entity_with_genotype"},{"id":"T6418","span":{"begin":584,"end":590},"obj":"SO_EXT:sequence_rescue_process"},{"id":"T6419","span":{"begin":592,"end":600},"obj":"SO_EXT:genotype_or_entity_with_genotype"},{"id":"T6420","span":{"begin":623,"end":631},"obj":"SO_EXT:sequence_rescue_process"},{"id":"T6421","span":{"begin":636,"end":644},"obj":"PATO_UBERON_EXT:neonate_or_newborn"},{"id":"T6422","span":{"begin":645,"end":651},"obj":"GO_EXT:fatality_or_lethality"},{"id":"T6423","span":{"begin":726,"end":733},"obj":"NCBITaxon:33208"},{"id":"T6424","span":{"begin":748,"end":758},"obj":"SO_EXT:0000902"},{"id":"T6425","span":{"begin":784,"end":799},"obj":"UBERON:0007221"}],"text":"Production of intermediate MSUD murine model\nThe various TRE-E2 transgenic lines produced were mated independently to mice that were heterozygous for both the E2 knockout and the LAP-tTA transgene [Tg(tTALap)Bjd/J; Stock 3272; The Jackson Laboratory, Bar Harbor, ME; NMRI × FVB × C57BL/6J background]. Interbreeding of animals that were heterozygous for both transgenes and the knockout resulted in the production of mice with a variety of genotypes including some animals that were homozygous for the knockout and were positive for both transgenes (we refer to this genotype as the \"rescue\" genotype). If our strategy for rescuing the neonatal lethal phenotype of the knockout were successful, then those homozygous knockout animals that had both transgenes would survive beyond the neonatal period."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T6386","span":{"begin":32,"end":38},"obj":"NCBITaxon:39107"},{"id":"T6387","span":{"begin":61,"end":63},"obj":"PR:000006300"},{"id":"T6388","span":{"begin":95,"end":100},"obj":"GO:0007618"},{"id":"T6389","span":{"begin":118,"end":122},"obj":"NCBITaxon:10088"},{"id":"T6390","span":{"begin":159,"end":161},"obj":"PR:000006300"},{"id":"T6391","span":{"begin":179,"end":182},"obj":"PR:000005308"},{"id":"T6392","span":{"begin":187,"end":196},"obj":"SO:0000902"},{"id":"T6393","span":{"begin":319,"end":326},"obj":"NCBITaxon:33208"},{"id":"T6394","span":{"begin":359,"end":369},"obj":"SO:0000902"},{"id":"T6395","span":{"begin":417,"end":421},"obj":"NCBITaxon:10088"},{"id":"T6396","span":{"begin":465,"end":472},"obj":"NCBITaxon:33208"},{"id":"T6397","span":{"begin":538,"end":548},"obj":"SO:0000902"},{"id":"T6398","span":{"begin":726,"end":733},"obj":"NCBITaxon:33208"},{"id":"T6399","span":{"begin":748,"end":758},"obj":"SO:0000902"},{"id":"T6400","span":{"begin":784,"end":799},"obj":"UBERON:0007221"}],"text":"Production of intermediate MSUD murine model\nThe various TRE-E2 transgenic lines produced were mated independently to mice that were heterozygous for both the E2 knockout and the LAP-tTA transgene [Tg(tTALap)Bjd/J; Stock 3272; The Jackson Laboratory, Bar Harbor, ME; NMRI × FVB × C57BL/6J background]. Interbreeding of animals that were heterozygous for both transgenes and the knockout resulted in the production of mice with a variety of genotypes including some animals that were homozygous for the knockout and were positive for both transgenes (we refer to this genotype as the \"rescue\" genotype). If our strategy for rescuing the neonatal lethal phenotype of the knockout were successful, then those homozygous knockout animals that had both transgenes would survive beyond the neonatal period."}