PMC:1447668 / 52561-53773 JSON TXT

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bionlp-st-ge-2016-reference-tees

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T23267","span":{"begin":28,"end":41},"obj":"Protein"},{"id":"T23268","span":{"begin":42,"end":52},"obj":"Gene_expression"},{"id":"T23269","span":{"begin":630,"end":635},"obj":"Protein"},{"id":"T23270","span":{"begin":641,"end":683},"obj":"Protein"},{"id":"T23271","span":{"begin":830,"end":844},"obj":"Protein"},{"id":"T23272","span":{"begin":968,"end":971},"obj":"Protein"},{"id":"T23273","span":{"begin":1017,"end":1021},"obj":"Protein"},{"id":"T23274","span":{"begin":972,"end":982},"obj":"Gene_expression"},{"id":"T23275","span":{"begin":1022,"end":1032},"obj":"Gene_expression"}],"relations":[{"id":"R17768","pred":"themeOf","subj":"T23267","obj":"T23268"},{"id":"R17769","pred":"themeOf","subj":"T23272","obj":"T23274"},{"id":"R17770","pred":"themeOf","subj":"T23273","obj":"T23275"}],"text":"Flow cytometric analysis of Foxp3 protein expression.\nCryopreserved PBMCs from HAM/TSP patients or HTLV-I-infected ACs were thawed and washed with FACS buffer (1× PBS, 0.1% NaN3, 5% FBS). Cells (1.5 × 106) were fixed by sequential formaldehyde/methanol fixation as follows. Cells were carefully resuspended in FACS buffer and fixed with 100 μl of reagent A (Fix \u0026 Perm kit; Caltag Laboratories, Burlingame, California, United States) at room temperature for 3 min followed by 2 ml of 70% methanol for 5 min at 4 °C. Cells were washed twice and permeabilized with 100 μl of reagent B (Fix \u0026 Perm kit) and stained for intracellular Foxp3 with mouse anti-human Foxp3 monoclonal antibody (0.5 μg of ab22510; Abcam) or the appropriate isotype control for 30 min. Cells were washed twice and stained with Cy5-conjugated goat anti-mouse immunoglobulin F(ab′)2 secondary antibody (Caltag Laboratories) for an additional 30 min. Cells were washed twice and stained for surface CD4 expression with PE-labeled anti-CD4 (BD) and CD25 expression with FITC-labeled anti-CD25 (BD). Cells were washed twice and analyzed on a FACSCalibur (BD). Data analysis was performed using FlowJo (Tree Star, Ashland, Oregon, United States)."}

sentences

{"project":"sentences","denotations":[{"id":"T22718","span":{"begin":0,"end":53},"obj":"Sentence"},{"id":"T22719","span":{"begin":54,"end":187},"obj":"Sentence"},{"id":"T22720","span":{"begin":188,"end":273},"obj":"Sentence"},{"id":"T22721","span":{"begin":274,"end":757},"obj":"Sentence"},{"id":"T22722","span":{"begin":758,"end":919},"obj":"Sentence"},{"id":"T22723","span":{"begin":920,"end":1066},"obj":"Sentence"},{"id":"T22724","span":{"begin":1067,"end":1126},"obj":"Sentence"},{"id":"T22725","span":{"begin":1127,"end":1212},"obj":"Sentence"},{"id":"T301","span":{"begin":0,"end":53},"obj":"Sentence"},{"id":"T302","span":{"begin":54,"end":187},"obj":"Sentence"},{"id":"T303","span":{"begin":188,"end":273},"obj":"Sentence"},{"id":"T304","span":{"begin":274,"end":515},"obj":"Sentence"},{"id":"T305","span":{"begin":516,"end":757},"obj":"Sentence"},{"id":"T306","span":{"begin":758,"end":919},"obj":"Sentence"},{"id":"T307","span":{"begin":920,"end":1066},"obj":"Sentence"},{"id":"T308","span":{"begin":1067,"end":1126},"obj":"Sentence"},{"id":"T309","span":{"begin":1127,"end":1212},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Flow cytometric analysis of Foxp3 protein expression.\nCryopreserved PBMCs from HAM/TSP patients or HTLV-I-infected ACs were thawed and washed with FACS buffer (1× PBS, 0.1% NaN3, 5% FBS). Cells (1.5 × 106) were fixed by sequential formaldehyde/methanol fixation as follows. Cells were carefully resuspended in FACS buffer and fixed with 100 μl of reagent A (Fix \u0026 Perm kit; Caltag Laboratories, Burlingame, California, United States) at room temperature for 3 min followed by 2 ml of 70% methanol for 5 min at 4 °C. Cells were washed twice and permeabilized with 100 μl of reagent B (Fix \u0026 Perm kit) and stained for intracellular Foxp3 with mouse anti-human Foxp3 monoclonal antibody (0.5 μg of ab22510; Abcam) or the appropriate isotype control for 30 min. Cells were washed twice and stained with Cy5-conjugated goat anti-mouse immunoglobulin F(ab′)2 secondary antibody (Caltag Laboratories) for an additional 30 min. Cells were washed twice and stained for surface CD4 expression with PE-labeled anti-CD4 (BD) and CD25 expression with FITC-labeled anti-CD25 (BD). Cells were washed twice and analyzed on a FACSCalibur (BD). Data analysis was performed using FlowJo (Tree Star, Ashland, Oregon, United States)."}

pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

GO-MF

{"project":"GO-MF","denotations":[{"id":"T23202","span":{"begin":675,"end":683},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T23203","span":{"begin":830,"end":844},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"Flow cytometric analysis of Foxp3 protein expression.\nCryopreserved PBMCs from HAM/TSP patients or HTLV-I-infected ACs were thawed and washed with FACS buffer (1× PBS, 0.1% NaN3, 5% FBS). Cells (1.5 × 106) were fixed by sequential formaldehyde/methanol fixation as follows. Cells were carefully resuspended in FACS buffer and fixed with 100 μl of reagent A (Fix \u0026 Perm kit; Caltag Laboratories, Burlingame, California, United States) at room temperature for 3 min followed by 2 ml of 70% methanol for 5 min at 4 °C. Cells were washed twice and permeabilized with 100 μl of reagent B (Fix \u0026 Perm kit) and stained for intracellular Foxp3 with mouse anti-human Foxp3 monoclonal antibody (0.5 μg of ab22510; Abcam) or the appropriate isotype control for 30 min. Cells were washed twice and stained with Cy5-conjugated goat anti-mouse immunoglobulin F(ab′)2 secondary antibody (Caltag Laboratories) for an additional 30 min. Cells were washed twice and stained for surface CD4 expression with PE-labeled anti-CD4 (BD) and CD25 expression with FITC-labeled anti-CD25 (BD). Cells were washed twice and analyzed on a FACSCalibur (BD). Data analysis was performed using FlowJo (Tree Star, Ashland, Oregon, United States)."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T23204","span":{"begin":616,"end":629},"obj":"http://purl.obolibrary.org/obo/GO_0005622"},{"id":"T23205","span":{"begin":675,"end":683},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T23206","span":{"begin":863,"end":871},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T23207","span":{"begin":675,"end":683},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T23208","span":{"begin":863,"end":871},"obj":"http://purl.obolibrary.org/obo/GO_0042571"}],"text":"Flow cytometric analysis of Foxp3 protein expression.\nCryopreserved PBMCs from HAM/TSP patients or HTLV-I-infected ACs were thawed and washed with FACS buffer (1× PBS, 0.1% NaN3, 5% FBS). Cells (1.5 × 106) were fixed by sequential formaldehyde/methanol fixation as follows. Cells were carefully resuspended in FACS buffer and fixed with 100 μl of reagent A (Fix \u0026 Perm kit; Caltag Laboratories, Burlingame, California, United States) at room temperature for 3 min followed by 2 ml of 70% methanol for 5 min at 4 °C. Cells were washed twice and permeabilized with 100 μl of reagent B (Fix \u0026 Perm kit) and stained for intracellular Foxp3 with mouse anti-human Foxp3 monoclonal antibody (0.5 μg of ab22510; Abcam) or the appropriate isotype control for 30 min. Cells were washed twice and stained with Cy5-conjugated goat anti-mouse immunoglobulin F(ab′)2 secondary antibody (Caltag Laboratories) for an additional 30 min. Cells were washed twice and stained for surface CD4 expression with PE-labeled anti-CD4 (BD) and CD25 expression with FITC-labeled anti-CD25 (BD). Cells were washed twice and analyzed on a FACSCalibur (BD). Data analysis was performed using FlowJo (Tree Star, Ashland, Oregon, United States)."}

events-check-again

{"project":"events-check-again","denotations":[{"id":"T23257","span":{"begin":28,"end":33},"obj":"Protein"},{"id":"T23258","span":{"begin":42,"end":52},"obj":"Gene_expression"},{"id":"T23259","span":{"begin":630,"end":635},"obj":"Protein"},{"id":"T23260","span":{"begin":658,"end":663},"obj":"Protein"},{"id":"T23261","span":{"begin":968,"end":971},"obj":"Protein"},{"id":"T23262","span":{"begin":972,"end":982},"obj":"Gene_expression"},{"id":"T23263","span":{"begin":1004,"end":1007},"obj":"Protein"},{"id":"T23264","span":{"begin":1017,"end":1021},"obj":"Protein"},{"id":"T23265","span":{"begin":1022,"end":1032},"obj":"Gene_expression"},{"id":"T23266","span":{"begin":1056,"end":1060},"obj":"Protein"}],"relations":[{"id":"R17765","pred":"themeOf","subj":"T23257","obj":"T23258"},{"id":"R17766","pred":"themeOf","subj":"T23261","obj":"T23262"},{"id":"R17767","pred":"themeOf","subj":"T23264","obj":"T23265"}],"attributes":[{"id":"M358","pred":"Speculation","subj":"T23262","obj":"true"},{"id":"M359","pred":"Speculation","subj":"T23265","obj":"true"},{"id":"M357","pred":"Speculation","subj":"T23258","obj":"true"}],"text":"Flow cytometric analysis of Foxp3 protein expression.\nCryopreserved PBMCs from HAM/TSP patients or HTLV-I-infected ACs were thawed and washed with FACS buffer (1× PBS, 0.1% NaN3, 5% FBS). Cells (1.5 × 106) were fixed by sequential formaldehyde/methanol fixation as follows. Cells were carefully resuspended in FACS buffer and fixed with 100 μl of reagent A (Fix \u0026 Perm kit; Caltag Laboratories, Burlingame, California, United States) at room temperature for 3 min followed by 2 ml of 70% methanol for 5 min at 4 °C. Cells were washed twice and permeabilized with 100 μl of reagent B (Fix \u0026 Perm kit) and stained for intracellular Foxp3 with mouse anti-human Foxp3 monoclonal antibody (0.5 μg of ab22510; Abcam) or the appropriate isotype control for 30 min. Cells were washed twice and stained with Cy5-conjugated goat anti-mouse immunoglobulin F(ab′)2 secondary antibody (Caltag Laboratories) for an additional 30 min. Cells were washed twice and stained for surface CD4 expression with PE-labeled anti-CD4 (BD) and CD25 expression with FITC-labeled anti-CD25 (BD). Cells were washed twice and analyzed on a FACSCalibur (BD). Data analysis was performed using FlowJo (Tree Star, Ashland, Oregon, United States)."}

bionlp-st-ge-2016-reference

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T22708","span":{"begin":28,"end":33},"obj":"Protein"},{"id":"T22709","span":{"begin":42,"end":52},"obj":"Gene_expression"},{"id":"T22710","span":{"begin":630,"end":635},"obj":"Protein"},{"id":"T22711","span":{"begin":658,"end":663},"obj":"Protein"},{"id":"T22712","span":{"begin":968,"end":971},"obj":"Protein"},{"id":"T22713","span":{"begin":972,"end":982},"obj":"Gene_expression"},{"id":"T22714","span":{"begin":1004,"end":1007},"obj":"Protein"},{"id":"T22715","span":{"begin":1017,"end":1021},"obj":"Protein"},{"id":"T22716","span":{"begin":1022,"end":1032},"obj":"Gene_expression"},{"id":"T22717","span":{"begin":1056,"end":1060},"obj":"Protein"}],"relations":[{"id":"R17281","pred":"themeOf","subj":"T22708","obj":"T22709"},{"id":"R17282","pred":"themeOf","subj":"T22712","obj":"T22713"},{"id":"R17283","pred":"themeOf","subj":"T22715","obj":"T22716"}],"attributes":[{"id":"M344","pred":"Speculation","subj":"T22713","obj":"true"},{"id":"M345","pred":"Speculation","subj":"T22716","obj":"true"},{"id":"M343","pred":"Speculation","subj":"T22709","obj":"true"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Flow cytometric analysis of Foxp3 protein expression.\nCryopreserved PBMCs from HAM/TSP patients or HTLV-I-infected ACs were thawed and washed with FACS buffer (1× PBS, 0.1% NaN3, 5% FBS). Cells (1.5 × 106) were fixed by sequential formaldehyde/methanol fixation as follows. Cells were carefully resuspended in FACS buffer and fixed with 100 μl of reagent A (Fix \u0026 Perm kit; Caltag Laboratories, Burlingame, California, United States) at room temperature for 3 min followed by 2 ml of 70% methanol for 5 min at 4 °C. Cells were washed twice and permeabilized with 100 μl of reagent B (Fix \u0026 Perm kit) and stained for intracellular Foxp3 with mouse anti-human Foxp3 monoclonal antibody (0.5 μg of ab22510; Abcam) or the appropriate isotype control for 30 min. Cells were washed twice and stained with Cy5-conjugated goat anti-mouse immunoglobulin F(ab′)2 secondary antibody (Caltag Laboratories) for an additional 30 min. Cells were washed twice and stained for surface CD4 expression with PE-labeled anti-CD4 (BD) and CD25 expression with FITC-labeled anti-CD25 (BD). Cells were washed twice and analyzed on a FACSCalibur (BD). Data analysis was performed using FlowJo (Tree Star, Ashland, Oregon, United States)."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T23187","span":{"begin":28,"end":33},"obj":"Q9BZS1"},{"id":"T23188","span":{"begin":630,"end":635},"obj":"Q9BZS1"},{"id":"T23189","span":{"begin":658,"end":663},"obj":"Q9BZS1"},{"id":"T23190","span":{"begin":968,"end":971},"obj":"P01730"},{"id":"T23191","span":{"begin":1004,"end":1007},"obj":"P01730"},{"id":"T23192","span":{"begin":1017,"end":1021},"obj":"P01589"},{"id":"T23193","span":{"begin":1056,"end":1060},"obj":"P01589"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Flow cytometric analysis of Foxp3 protein expression.\nCryopreserved PBMCs from HAM/TSP patients or HTLV-I-infected ACs were thawed and washed with FACS buffer (1× PBS, 0.1% NaN3, 5% FBS). Cells (1.5 × 106) were fixed by sequential formaldehyde/methanol fixation as follows. Cells were carefully resuspended in FACS buffer and fixed with 100 μl of reagent A (Fix \u0026 Perm kit; Caltag Laboratories, Burlingame, California, United States) at room temperature for 3 min followed by 2 ml of 70% methanol for 5 min at 4 °C. Cells were washed twice and permeabilized with 100 μl of reagent B (Fix \u0026 Perm kit) and stained for intracellular Foxp3 with mouse anti-human Foxp3 monoclonal antibody (0.5 μg of ab22510; Abcam) or the appropriate isotype control for 30 min. Cells were washed twice and stained with Cy5-conjugated goat anti-mouse immunoglobulin F(ab′)2 secondary antibody (Caltag Laboratories) for an additional 30 min. Cells were washed twice and stained for surface CD4 expression with PE-labeled anti-CD4 (BD) and CD25 expression with FITC-labeled anti-CD25 (BD). Cells were washed twice and analyzed on a FACSCalibur (BD). Data analysis was performed using FlowJo (Tree Star, Ashland, Oregon, United States)."}

test2

{"project":"test2","denotations":[{"id":"T22698","span":{"begin":28,"end":33},"obj":"Protein"},{"id":"T22699","span":{"begin":42,"end":52},"obj":"Gene_expression"},{"id":"T22700","span":{"begin":630,"end":635},"obj":"Protein"},{"id":"T22701","span":{"begin":658,"end":663},"obj":"Protein"},{"id":"T22702","span":{"begin":968,"end":971},"obj":"Protein"},{"id":"T22703","span":{"begin":972,"end":982},"obj":"Gene_expression"},{"id":"T22704","span":{"begin":1004,"end":1007},"obj":"Protein"},{"id":"T22705","span":{"begin":1017,"end":1021},"obj":"Protein"},{"id":"T22706","span":{"begin":1022,"end":1032},"obj":"Gene_expression"},{"id":"T22707","span":{"begin":1056,"end":1060},"obj":"Protein"}],"relations":[{"id":"R17277","pred":"themeOf","subj":"T22698","obj":"T22699"},{"id":"R17278","pred":"themeOf","subj":"T22702","obj":"T22703"},{"id":"R17279","pred":"themeOf","subj":"T22704","obj":"T22706"},{"id":"R17280","pred":"themeOf","subj":"T22705","obj":"T22706"}],"attributes":[{"id":"M340","pred":"Speculation","subj":"T22698","obj":"true"},{"id":"M341","pred":"Speculation","subj":"T22699","obj":"true"},{"id":"M342","pred":"Speculation","subj":"T22700","obj":"true"}],"text":"Flow cytometric analysis of Foxp3 protein expression.\nCryopreserved PBMCs from HAM/TSP patients or HTLV-I-infected ACs were thawed and washed with FACS buffer (1× PBS, 0.1% NaN3, 5% FBS). Cells (1.5 × 106) were fixed by sequential formaldehyde/methanol fixation as follows. Cells were carefully resuspended in FACS buffer and fixed with 100 μl of reagent A (Fix \u0026 Perm kit; Caltag Laboratories, Burlingame, California, United States) at room temperature for 3 min followed by 2 ml of 70% methanol for 5 min at 4 °C. Cells were washed twice and permeabilized with 100 μl of reagent B (Fix \u0026 Perm kit) and stained for intracellular Foxp3 with mouse anti-human Foxp3 monoclonal antibody (0.5 μg of ab22510; Abcam) or the appropriate isotype control for 30 min. Cells were washed twice and stained with Cy5-conjugated goat anti-mouse immunoglobulin F(ab′)2 secondary antibody (Caltag Laboratories) for an additional 30 min. Cells were washed twice and stained for surface CD4 expression with PE-labeled anti-CD4 (BD) and CD25 expression with FITC-labeled anti-CD25 (BD). Cells were washed twice and analyzed on a FACSCalibur (BD). Data analysis was performed using FlowJo (Tree Star, Ashland, Oregon, United States)."}

PMC:1447668 / 52561-53773 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:1447668 / 52561-53773 JSON TXT