PMC:1435744 / 654-1925 JSONTXT

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T927","span":{"begin":8,"end":12},"obj":"PR_EXT:000014441"},{"id":"T928","span":{"begin":16,"end":40},"obj":"SO_EXT:biological_conservation_process_or_quality"},{"id":"T929","span":{"begin":46,"end":55},"obj":"NCBITaxon:7955"},{"id":"T930","span":{"begin":64,"end":69},"obj":"NCBITaxon:9606"},{"id":"T931","span":{"begin":71,"end":80},"obj":"NCBITaxon:1"},{"id":"T932","span":{"begin":112,"end":125},"obj":"CL:0000210"},{"id":"T933","span":{"begin":112,"end":137},"obj":"GO:0042461"},{"id":"T934","span":{"begin":153,"end":162},"obj":"SO_EXT:biological_conservation_process_or_quality"},{"id":"T935","span":{"begin":208,"end":214},"obj":"SO_EXT:0000417"},{"id":"T936","span":{"begin":218,"end":222},"obj":"PR_EXT:000014441"},{"id":"T937","span":{"begin":252,"end":257},"obj":"NCBITaxon:10088"},{"id":"T938","span":{"begin":276,"end":284},"obj":"SO_EXT:0000855"},{"id":"T939","span":{"begin":286,"end":290},"obj":"PR_EXT:Q64028"},{"id":"T940","span":{"begin":291,"end":296},"obj":"PR_EXT:000012633"},{"id":"T941","span":{"begin":319,"end":329},"obj":"SO_EXT:epigenetic_modification_entity_or_process"},{"id":"T942","span":{"begin":330,"end":338},"obj":"CHEBI:36357"},{"id":"T943","span":{"begin":351,"end":360},"obj":"GO:0000785"},{"id":"T944","span":{"begin":351,"end":374},"obj":"GO:0016568"},{"id":"T945","span":{"begin":361,"end":374},"obj":"SO_EXT:sequence_alteration_entity_or_process"},{"id":"T946","span":{"begin":420,"end":424},"obj":"PR_EXT:000014441"},{"id":"T947","span":{"begin":453,"end":463},"obj":"GO:0065007"},{"id":"T948","span":{"begin":464,"end":468},"obj":"SO_EXT:0000704"},{"id":"T949","span":{"begin":464,"end":479},"obj":"GO:0010467"},{"id":"T950","span":{"begin":483,"end":496},"obj":"CL:0000210"},{"id":"T951","span":{"begin":483,"end":508},"obj":"GO:0042461"},{"id":"T952","span":{"begin":510,"end":514},"obj":"PR_EXT:000014441"},{"id":"T953","span":{"begin":533,"end":542},"obj":"GO:0010467"},{"id":"T954","span":{"begin":550,"end":564},"obj":"CL:0000210"},{"id":"T955","span":{"begin":572,"end":577},"obj":"GO:0007567"},{"id":"T956","span":{"begin":599,"end":613},"obj":"CL:0000210"},{"id":"T957","span":{"begin":631,"end":646},"obj":"GO_RO_EXT:developmental_differentiation_process"},{"id":"T958","span":{"begin":659,"end":664},"obj":"UBERON:0007023"},{"id":"T959","span":{"begin":665,"end":677},"obj":"UBERON:0001905"},{"id":"T960","span":{"begin":679,"end":692},"obj":"GO_EXT:transcription"},{"id":"T961","span":{"begin":696,"end":700},"obj":"PR_EXT:000014441"},{"id":"T962","span":{"begin":713,"end":722},"obj":"GO:0065007"},{"id":"T963","span":{"begin":730,"end":734},"obj":"CL:0000573"},{"id":"T964","span":{"begin":735,"end":738},"obj":"CL:0000604"},{"id":"T965","span":{"begin":751,"end":758},"obj":"CHEBI_PR_EXT:protein"},{"id":"T966","span":{"begin":759,"end":762},"obj":"PR_EXT:000005904"},{"id":"T967","span":{"begin":806,"end":810},"obj":"PR_EXT:000014441"},{"id":"T968","span":{"begin":811,"end":818},"obj":"CHEBI_PR_EXT:protein"},{"id":"T969","span":{"begin":858,"end":864},"obj":"SO_EXT:0000417"},{"id":"T970","span":{"begin":902,"end":906},"obj":"PR_EXT:000014441"},{"id":"T971","span":{"begin":907,"end":914},"obj":"CHEBI_PR_EXT:protein"},{"id":"T972","span":{"begin":915,"end":924},"obj":"GO_PATO_EXT:biological_localization_process_or_quality"},{"id":"T973","span":{"begin":939,"end":946},"obj":"GO:0005634"},{"id":"T974","span":{"begin":953,"end":957},"obj":"PR_EXT:000014441"},{"id":"T975","span":{"begin":965,"end":974},"obj":"GO:0010467"},{"id":"T976","span":{"begin":986,"end":991},"obj":"CL_GO_EXT:cell"},{"id":"T977","span":{"begin":1010,"end":1020},"obj":"GO_EXT:0045289"},{"id":"T978","span":{"begin":1043,"end":1047},"obj":"PR_EXT:000014441"},{"id":"T979","span":{"begin":1048,"end":1055},"obj":"CHEBI_PR_EXT:protein"},{"id":"T980","span":{"begin":1056,"end":1061},"obj":"SO_EXT:sequence_fusion_process"},{"id":"T981","span":{"begin":1065,"end":1069},"obj":"PR_EXT:P04386"},{"id":"T982","span":{"begin":1070,"end":1073},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T983","span":{"begin":1074,"end":1081},"obj":"CHEMINF_GO_EXT:chemical_binding_or_bond_formation"},{"id":"T984","span":{"begin":1082,"end":1088},"obj":"SO_EXT:0000417"},{"id":"T985","span":{"begin":1104,"end":1119},"obj":"GO_EXT:transcription"},{"id":"T986","span":{"begin":1175,"end":1179},"obj":"PR_EXT:000014441"},{"id":"T987","span":{"begin":1235,"end":1241},"obj":"SO_EXT:0000417"},{"id":"T988","span":{"begin":1253,"end":1263},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"}],"text":"Results\nmr-s is evolutionarily conserved from zebrafish through human, organisms through which the mechanism of photoreceptor development is also highly conserved. Phylogenetic analysis suggests that the SAM domain of mr-s is most closely related to a mouse polyhomeotic (ph) ortholog, Mph1/Rae28, which is known as an epigenetic molecule involved in chromatin modifications. These findings provide the possibility that mr-s may play a critical role by regulating gene expression in photoreceptor development. mr-s is preferentially expressed in the photoreceptors at postnatal day 3–6 (P3-6), when photoreceptors undergo terminal differentiation, and in the adult pineal gland. Transcription of mr-s is directly regulated by the cone-rod homeodomain protein Crx. Immunoprecipitation assay showed that the mr-s protein self-associates mainly through the SAM domain-containing region as well as ph. The mr-s protein localizes mainly in the nucleus, when mr-s is overexpressed in HEK293T cells. Moreover, in the luciferase assays, we found that mr-s protein fused to GAL4 DNA-binding domain functions as a transcriptional repressor. We revealed that the repression activity of mr-s is not due to a homophilic interaction through its SAM domain but to the C-terminal region."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T840","span":{"begin":8,"end":12},"obj":"PR:000014441"},{"id":"T841","span":{"begin":46,"end":55},"obj":"NCBITaxon:7955"},{"id":"T842","span":{"begin":64,"end":69},"obj":"NCBITaxon:9606"},{"id":"T843","span":{"begin":71,"end":80},"obj":"NCBITaxon:1"},{"id":"T844","span":{"begin":112,"end":125},"obj":"CL:0000210"},{"id":"T845","span":{"begin":112,"end":137},"obj":"GO:0042461"},{"id":"T846","span":{"begin":208,"end":214},"obj":"SO:0000417"},{"id":"T847","span":{"begin":218,"end":222},"obj":"PR:000014441"},{"id":"T848","span":{"begin":252,"end":257},"obj":"NCBITaxon:10088"},{"id":"T849","span":{"begin":276,"end":284},"obj":"SO:0000855"},{"id":"T850","span":{"begin":286,"end":290},"obj":"PR:Q64028"},{"id":"T851","span":{"begin":291,"end":296},"obj":"PR:000012633"},{"id":"T852","span":{"begin":330,"end":338},"obj":"CHEBI:36357"},{"id":"T853","span":{"begin":351,"end":360},"obj":"GO:0000785"},{"id":"T854","span":{"begin":351,"end":374},"obj":"GO:0016568"},{"id":"T855","span":{"begin":420,"end":424},"obj":"PR:000014441"},{"id":"T856","span":{"begin":453,"end":463},"obj":"GO:0065007"},{"id":"T857","span":{"begin":464,"end":468},"obj":"SO:0000704"},{"id":"T858","span":{"begin":464,"end":479},"obj":"GO:0010467"},{"id":"T859","span":{"begin":483,"end":496},"obj":"CL:0000210"},{"id":"T860","span":{"begin":483,"end":508},"obj":"GO:0042461"},{"id":"T861","span":{"begin":510,"end":514},"obj":"PR:000014441"},{"id":"T862","span":{"begin":533,"end":542},"obj":"GO:0010467"},{"id":"T863","span":{"begin":550,"end":564},"obj":"CL:0000210"},{"id":"T864","span":{"begin":572,"end":577},"obj":"GO:0007567"},{"id":"T865","span":{"begin":599,"end":613},"obj":"CL:0000210"},{"id":"T866","span":{"begin":659,"end":664},"obj":"UBERON:0007023"},{"id":"T867","span":{"begin":665,"end":677},"obj":"UBERON:0001905"},{"id":"T868","span":{"begin":696,"end":700},"obj":"PR:000014441"},{"id":"T869","span":{"begin":713,"end":722},"obj":"GO:0065007"},{"id":"T870","span":{"begin":730,"end":734},"obj":"CL:0000573"},{"id":"T871","span":{"begin":735,"end":738},"obj":"CL:0000604"},{"id":"T872","span":{"begin":759,"end":762},"obj":"PR:000005904"},{"id":"T873","span":{"begin":806,"end":810},"obj":"PR:000014441"},{"id":"T874","span":{"begin":858,"end":864},"obj":"SO:0000417"},{"id":"T875","span":{"begin":902,"end":906},"obj":"PR:000014441"},{"id":"T876","span":{"begin":939,"end":946},"obj":"GO:0005634"},{"id":"T877","span":{"begin":953,"end":957},"obj":"PR:000014441"},{"id":"T878","span":{"begin":965,"end":974},"obj":"GO:0010467"},{"id":"T879","span":{"begin":1043,"end":1047},"obj":"PR:000014441"},{"id":"T880","span":{"begin":1065,"end":1069},"obj":"PR:P04386"},{"id":"T881","span":{"begin":1082,"end":1088},"obj":"SO:0000417"},{"id":"T882","span":{"begin":1175,"end":1179},"obj":"PR:000014441"},{"id":"T883","span":{"begin":1235,"end":1241},"obj":"SO:0000417"}],"text":"Results\nmr-s is evolutionarily conserved from zebrafish through human, organisms through which the mechanism of photoreceptor development is also highly conserved. Phylogenetic analysis suggests that the SAM domain of mr-s is most closely related to a mouse polyhomeotic (ph) ortholog, Mph1/Rae28, which is known as an epigenetic molecule involved in chromatin modifications. These findings provide the possibility that mr-s may play a critical role by regulating gene expression in photoreceptor development. mr-s is preferentially expressed in the photoreceptors at postnatal day 3–6 (P3-6), when photoreceptors undergo terminal differentiation, and in the adult pineal gland. Transcription of mr-s is directly regulated by the cone-rod homeodomain protein Crx. Immunoprecipitation assay showed that the mr-s protein self-associates mainly through the SAM domain-containing region as well as ph. The mr-s protein localizes mainly in the nucleus, when mr-s is overexpressed in HEK293T cells. Moreover, in the luciferase assays, we found that mr-s protein fused to GAL4 DNA-binding domain functions as a transcriptional repressor. We revealed that the repression activity of mr-s is not due to a homophilic interaction through its SAM domain but to the C-terminal region."}

{"project":"craft-sa-dev","denotations":[{"id":"T1125","span":{"begin":0,"end":7},"obj":"NNS"},{"id":"T1126","span":{"begin":7,"end":163},"obj":"sentence"},{"id":"T1127","span":{"begin":8,"end":10},"obj":"NN"},{"id":"T1128","span":{"begin":11,"end":12},"obj":"NN"},{"id":"T1129","span":{"begin":10,"end":11},"obj":"HYPH"},{"id":"T1130","span":{"begin":31,"end":40},"obj":"VBN"},{"id":"T1131","span":{"begin":13,"end":15},"obj":"VBZ"},{"id":"T1132","span":{"begin":16,"end":30},"obj":"RB"},{"id":"T1133","span":{"begin":41,"end":45},"obj":"IN"},{"id":"T1134","span":{"begin":46,"end":55},"obj":"NN"},{"id":"T1135","span":{"begin":56,"end":63},"obj":"IN"},{"id":"T1136","span":{"begin":64,"end":69},"obj":"JJ"},{"id":"T1137","span":{"begin":69,"end":71},"obj":","},{"id":"T1138","span":{"begin":71,"end":80},"obj":"NNS"},{"id":"T1139","span":{"begin":81,"end":88},"obj":"IN"},{"id":"T1140","span":{"begin":153,"end":162},"obj":"VBN"},{"id":"T1141","span":{"begin":89,"end":94},"obj":"WDT"},{"id":"T1142","span":{"begin":95,"end":98},"obj":"DT"},{"id":"T1143","span":{"begin":99,"end":108},"obj":"NN"},{"id":"T1144","span":{"begin":109,"end":111},"obj":"IN"},{"id":"T1145","span":{"begin":112,"end":125},"obj":"NN"},{"id":"T1146","span":{"begin":126,"end":137},"obj":"NN"},{"id":"T1147","span":{"begin":138,"end":140},"obj":"VBZ"},{"id":"T1148","span":{"begin":141,"end":145},"obj":"RB"},{"id":"T1149","span":{"begin":146,"end":152},"obj":"RB"},{"id":"T1150","span":{"begin":162,"end":163},"obj":"."},{"id":"T1151","span":{"begin":163,"end":375},"obj":"sentence"},{"id":"T1152","span":{"begin":164,"end":176},"obj":"JJ"},{"id":"T1153","span":{"begin":177,"end":185},"obj":"NN"},{"id":"T1154","span":{"begin":186,"end":194},"obj":"VBZ"},{"id":"T1155","span":{"begin":195,"end":199},"obj":"IN"},{"id":"T1156","span":{"begin":239,"end":246},"obj":"VBN"},{"id":"T1157","span":{"begin":200,"end":203},"obj":"DT"},{"id":"T1158","span":{"begin":208,"end":214},"obj":"NN"},{"id":"T1159","span":{"begin":204,"end":207},"obj":"NN"},{"id":"T1160","span":{"begin":215,"end":217},"obj":"IN"},{"id":"T1161","span":{"begin":218,"end":220},"obj":"NN"},{"id":"T1162","span":{"begin":221,"end":222},"obj":"NN"},{"id":"T1163","span":{"begin":220,"end":221},"obj":"HYPH"},{"id":"T1164","span":{"begin":223,"end":225},"obj":"VBZ"},{"id":"T1165","span":{"begin":226,"end":230},"obj":"RBS"},{"id":"T1166","span":{"begin":231,"end":238},"obj":"RB"},{"id":"T1167","span":{"begin":247,"end":249},"obj":"IN"},{"id":"T1168","span":{"begin":250,"end":251},"obj":"DT"},{"id":"T1169","span":{"begin":276,"end":284},"obj":"NN"},{"id":"T1170","span":{"begin":252,"end":257},"obj":"NN"},{"id":"T1171","span":{"begin":258,"end":270},"obj":"NN"},{"id":"T1172","span":{"begin":271,"end":272},"obj":"-LRB-"},{"id":"T1173","span":{"begin":272,"end":274},"obj":"NN"},{"id":"T1174","span":{"begin":274,"end":275},"obj":"-RRB-"},{"id":"T1175","span":{"begin":284,"end":286},"obj":","},{"id":"T1176","span":{"begin":286,"end":290},"obj":"NN"},{"id":"T1177","span":{"begin":291,"end":296},"obj":"NN"},{"id":"T1178","span":{"begin":290,"end":291},"obj":"HYPH"},{"id":"T1179","span":{"begin":296,"end":298},"obj":","},{"id":"T1180","span":{"begin":298,"end":303},"obj":"WDT"},{"id":"T1181","span":{"begin":307,"end":312},"obj":"VBN"},{"id":"T1182","span":{"begin":304,"end":306},"obj":"VBZ"},{"id":"T1183","span":{"begin":313,"end":315},"obj":"IN"},{"id":"T1184","span":{"begin":316,"end":318},"obj":"DT"},{"id":"T1185","span":{"begin":330,"end":338},"obj":"NN"},{"id":"T1186","span":{"begin":319,"end":329},"obj":"JJ"},{"id":"T1187","span":{"begin":339,"end":347},"obj":"VBN"},{"id":"T1188","span":{"begin":348,"end":350},"obj":"IN"},{"id":"T1189","span":{"begin":351,"end":360},"obj":"NN"},{"id":"T1190","span":{"begin":361,"end":374},"obj":"NNS"},{"id":"T1191","span":{"begin":374,"end":375},"obj":"."},{"id":"T1192","span":{"begin":375,"end":509},"obj":"sentence"},{"id":"T1193","span":{"begin":376,"end":381},"obj":"DT"},{"id":"T1194","span":{"begin":382,"end":390},"obj":"NNS"},{"id":"T1195","span":{"begin":391,"end":398},"obj":"VBP"},{"id":"T1196","span":{"begin":399,"end":402},"obj":"DT"},{"id":"T1197","span":{"begin":403,"end":414},"obj":"NN"},{"id":"T1198","span":{"begin":415,"end":419},"obj":"IN"},{"id":"T1199","span":{"begin":429,"end":433},"obj":"VB"},{"id":"T1200","span":{"begin":420,"end":422},"obj":"NN"},{"id":"T1201","span":{"begin":423,"end":424},"obj":"NN"},{"id":"T1202","span":{"begin":422,"end":423},"obj":"HYPH"},{"id":"T1203","span":{"begin":425,"end":428},"obj":"MD"},{"id":"T1204","span":{"begin":434,"end":435},"obj":"DT"},{"id":"T1205","span":{"begin":445,"end":449},"obj":"NN"},{"id":"T1206","span":{"begin":436,"end":444},"obj":"JJ"},{"id":"T1207","span":{"begin":450,"end":452},"obj":"IN"},{"id":"T1208","span":{"begin":453,"end":463},"obj":"VBG"},{"id":"T1209","span":{"begin":464,"end":468},"obj":"NN"},{"id":"T1210","span":{"begin":469,"end":479},"obj":"NN"},{"id":"T1211","span":{"begin":480,"end":482},"obj":"IN"},{"id":"T1212","span":{"begin":483,"end":496},"obj":"NN"},{"id":"T1213","span":{"begin":497,"end":508},"obj":"NN"},{"id":"T1214","span":{"begin":508,"end":509},"obj":"."},{"id":"T1215","span":{"begin":509,"end":678},"obj":"sentence"},{"id":"T1216","span":{"begin":510,"end":512},"obj":"NN"},{"id":"T1217","span":{"begin":513,"end":514},"obj":"NN"},{"id":"T1218","span":{"begin":512,"end":513},"obj":"HYPH"},{"id":"T1219","span":{"begin":533,"end":542},"obj":"VBN"},{"id":"T1220","span":{"begin":515,"end":517},"obj":"VBZ"},{"id":"T1221","span":{"begin":518,"end":532},"obj":"RB"},{"id":"T1222","span":{"begin":543,"end":545},"obj":"IN"},{"id":"T1223","span":{"begin":546,"end":549},"obj":"DT"},{"id":"T1224","span":{"begin":550,"end":564},"obj":"NNS"},{"id":"T1225","span":{"begin":565,"end":567},"obj":"IN"},{"id":"T1226","span":{"begin":568,"end":577},"obj":"JJ"},{"id":"T1227","span":{"begin":582,"end":583},"obj":"CD"},{"id":"T1228","span":{"begin":578,"end":581},"obj":"NN"},{"id":"T1229","span":{"begin":583,"end":584},"obj":"SYM"},{"id":"T1230","span":{"begin":584,"end":585},"obj":"CD"},{"id":"T1231","span":{"begin":586,"end":587},"obj":"-LRB-"},{"id":"T1232","span":{"begin":587,"end":589},"obj":"NN"},{"id":"T1233","span":{"begin":589,"end":590},"obj":"SYM"},{"id":"T1234","span":{"begin":590,"end":591},"obj":"CD"},{"id":"T1235","span":{"begin":591,"end":592},"obj":"-RRB-"},{"id":"T1236","span":{"begin":592,"end":594},"obj":","},{"id":"T1237","span":{"begin":594,"end":598},"obj":"WRB"},{"id":"T1238","span":{"begin":614,"end":621},"obj":"VBP"},{"id":"T1239","span":{"begin":599,"end":613},"obj":"NNS"},{"id":"T1240","span":{"begin":622,"end":630},"obj":"JJ"},{"id":"T1241","span":{"begin":631,"end":646},"obj":"NN"},{"id":"T1242","span":{"begin":646,"end":648},"obj":","},{"id":"T1243","span":{"begin":648,"end":651},"obj":"CC"},{"id":"T1244","span":{"begin":652,"end":654},"obj":"IN"},{"id":"T1245","span":{"begin":655,"end":658},"obj":"DT"},{"id":"T1246","span":{"begin":672,"end":677},"obj":"NN"},{"id":"T1247","span":{"begin":659,"end":664},"obj":"JJ"},{"id":"T1248","span":{"begin":665,"end":671},"obj":"JJ"},{"id":"T1249","span":{"begin":677,"end":678},"obj":"."},{"id":"T1250","span":{"begin":678,"end":763},"obj":"sentence"},{"id":"T1251","span":{"begin":679,"end":692},"obj":"NN"},{"id":"T1252","span":{"begin":713,"end":722},"obj":"VBN"},{"id":"T1253","span":{"begin":693,"end":695},"obj":"IN"},{"id":"T1254","span":{"begin":696,"end":698},"obj":"NN"},{"id":"T1255","span":{"begin":699,"end":700},"obj":"NN"},{"id":"T1256","span":{"begin":698,"end":699},"obj":"HYPH"},{"id":"T1257","span":{"begin":701,"end":703},"obj":"VBZ"},{"id":"T1258","span":{"begin":704,"end":712},"obj":"RB"},{"id":"T1259","span":{"begin":723,"end":725},"obj":"IN"},{"id":"T1260","span":{"begin":726,"end":729},"obj":"DT"},{"id":"T1261","span":{"begin":751,"end":758},"obj":"NN"},{"id":"T1262","span":{"begin":730,"end":734},"obj":"NN"},{"id":"T1263","span":{"begin":735,"end":738},"obj":"NN"},{"id":"T1264","span":{"begin":734,"end":735},"obj":"HYPH"},{"id":"T1265","span":{"begin":739,"end":750},"obj":"NN"},{"id":"T1266","span":{"begin":759,"end":762},"obj":"NN"},{"id":"T1267","span":{"begin":762,"end":763},"obj":"."},{"id":"T1268","span":{"begin":763,"end":897},"obj":"sentence"},{"id":"T1269","span":{"begin":764,"end":783},"obj":"NN"},{"id":"T1270","span":{"begin":784,"end":789},"obj":"NN"},{"id":"T1271","span":{"begin":790,"end":796},"obj":"VBD"},{"id":"T1272","span":{"begin":797,"end":801},"obj":"IN"},{"id":"T1273","span":{"begin":824,"end":834},"obj":"VBZ"},{"id":"T1274","span":{"begin":802,"end":805},"obj":"DT"},{"id":"T1275","span":{"begin":811,"end":818},"obj":"NN"},{"id":"T1276","span":{"begin":806,"end":808},"obj":"NN"},{"id":"T1277","span":{"begin":809,"end":810},"obj":"NN"},{"id":"T1278","span":{"begin":808,"end":809},"obj":"HYPH"},{"id":"T1279","span":{"begin":819,"end":823},"obj":"NN"},{"id":"T1280","span":{"begin":823,"end":824},"obj":"HYPH"},{"id":"T1281","span":{"begin":835,"end":841},"obj":"RB"},{"id":"T1282","span":{"begin":842,"end":849},"obj":"IN"},{"id":"T1283","span":{"begin":850,"end":853},"obj":"DT"},{"id":"T1284","span":{"begin":876,"end":882},"obj":"NN"},{"id":"T1285","span":{"begin":854,"end":857},"obj":"NN"},{"id":"T1286","span":{"begin":858,"end":864},"obj":"NN"},{"id":"T1287","span":{"begin":865,"end":875},"obj":"VBG"},{"id":"T1288","span":{"begin":864,"end":865},"obj":"HYPH"},{"id":"T1289","span":{"begin":883,"end":885},"obj":"RB"},{"id":"T1290","span":{"begin":891,"end":893},"obj":"IN"},{"id":"T1291","span":{"begin":886,"end":890},"obj":"RB"},{"id":"T1292","span":{"begin":894,"end":896},"obj":"NN"},{"id":"T1293","span":{"begin":896,"end":897},"obj":"."},{"id":"T1294","span":{"begin":897,"end":992},"obj":"sentence"},{"id":"T1295","span":{"begin":898,"end":901},"obj":"DT"},{"id":"T1296","span":{"begin":907,"end":914},"obj":"NN"},{"id":"T1297","span":{"begin":902,"end":904},"obj":"NN"},{"id":"T1298","span":{"begin":905,"end":906},"obj":"NN"},{"id":"T1299","span":{"begin":904,"end":905},"obj":"HYPH"},{"id":"T1300","span":{"begin":915,"end":924},"obj":"VBZ"},{"id":"T1301","span":{"begin":925,"end":931},"obj":"RB"},{"id":"T1302","span":{"begin":932,"end":934},"obj":"IN"},{"id":"T1303","span":{"begin":935,"end":938},"obj":"DT"},{"id":"T1304","span":{"begin":939,"end":946},"obj":"NN"},{"id":"T1305","span":{"begin":946,"end":948},"obj":","},{"id":"T1306","span":{"begin":948,"end":952},"obj":"WRB"},{"id":"T1307","span":{"begin":961,"end":974},"obj":"VBN"},{"id":"T1308","span":{"begin":953,"end":955},"obj":"NN"},{"id":"T1309","span":{"begin":956,"end":957},"obj":"NN"},{"id":"T1310","span":{"begin":955,"end":956},"obj":"HYPH"},{"id":"T1311","span":{"begin":958,"end":960},"obj":"VBZ"},{"id":"T1312","span":{"begin":975,"end":977},"obj":"IN"},{"id":"T1313","span":{"begin":978,"end":985},"obj":"NN"},{"id":"T1314","span":{"begin":986,"end":991},"obj":"NNS"},{"id":"T1315","span":{"begin":991,"end":992},"obj":"."},{"id":"T1316","span":{"begin":992,"end":1130},"obj":"sentence"},{"id":"T1317","span":{"begin":993,"end":1001},"obj":"RB"},{"id":"T1318","span":{"begin":1032,"end":1037},"obj":"VBD"},{"id":"T1319","span":{"begin":1001,"end":1003},"obj":","},{"id":"T1320","span":{"begin":1003,"end":1005},"obj":"IN"},{"id":"T1321","span":{"begin":1006,"end":1009},"obj":"DT"},{"id":"T1322","span":{"begin":1021,"end":1027},"obj":"NNS"},{"id":"T1323","span":{"begin":1010,"end":1020},"obj":"NN"},{"id":"T1324","span":{"begin":1027,"end":1029},"obj":","},{"id":"T1325","span":{"begin":1029,"end":1031},"obj":"PRP"},{"id":"T1326","span":{"begin":1038,"end":1042},"obj":"IN"},{"id":"T1327","span":{"begin":1089,"end":1098},"obj":"VBZ"},{"id":"T1328","span":{"begin":1043,"end":1045},"obj":"NN"},{"id":"T1329","span":{"begin":1046,"end":1047},"obj":"NN"},{"id":"T1330","span":{"begin":1045,"end":1046},"obj":"HYPH"},{"id":"T1331","span":{"begin":1048,"end":1055},"obj":"NN"},{"id":"T1332","span":{"begin":1056,"end":1061},"obj":"VBN"},{"id":"T1333","span":{"begin":1062,"end":1064},"obj":"IN"},{"id":"T1334","span":{"begin":1065,"end":1069},"obj":"NN"},{"id":"T1335","span":{"begin":1082,"end":1088},"obj":"NN"},{"id":"T1336","span":{"begin":1070,"end":1073},"obj":"NN"},{"id":"T1337","span":{"begin":1074,"end":1081},"obj":"VBG"},{"id":"T1338","span":{"begin":1073,"end":1074},"obj":"HYPH"},{"id":"T1339","span":{"begin":1099,"end":1101},"obj":"IN"},{"id":"T1340","span":{"begin":1102,"end":1103},"obj":"DT"},{"id":"T1341","span":{"begin":1120,"end":1129},"obj":"NN"},{"id":"T1342","span":{"begin":1104,"end":1119},"obj":"JJ"},{"id":"T1343","span":{"begin":1129,"end":1130},"obj":"."},{"id":"T1344","span":{"begin":1130,"end":1271},"obj":"sentence"},{"id":"T1345","span":{"begin":1131,"end":1133},"obj":"PRP"},{"id":"T1346","span":{"begin":1134,"end":1142},"obj":"VBD"},{"id":"T1347","span":{"begin":1143,"end":1147},"obj":"IN"},{"id":"T1348","span":{"begin":1180,"end":1182},"obj":"VBZ"},{"id":"T1349","span":{"begin":1148,"end":1151},"obj":"DT"},{"id":"T1350","span":{"begin":1163,"end":1171},"obj":"NN"},{"id":"T1351","span":{"begin":1152,"end":1162},"obj":"NN"},{"id":"T1352","span":{"begin":1172,"end":1174},"obj":"IN"},{"id":"T1353","span":{"begin":1175,"end":1177},"obj":"NN"},{"id":"T1354","span":{"begin":1178,"end":1179},"obj":"NN"},{"id":"T1355","span":{"begin":1177,"end":1178},"obj":"HYPH"},{"id":"T1356","span":{"begin":1183,"end":1186},"obj":"RB"},{"id":"T1357","span":{"begin":1187,"end":1190},"obj":"IN"},{"id":"T1358","span":{"begin":1191,"end":1193},"obj":"IN"},{"id":"T1359","span":{"begin":1194,"end":1195},"obj":"DT"},{"id":"T1360","span":{"begin":1207,"end":1218},"obj":"NN"},{"id":"T1361","span":{"begin":1196,"end":1206},"obj":"JJ"},{"id":"T1362","span":{"begin":1219,"end":1226},"obj":"IN"},{"id":"T1363","span":{"begin":1227,"end":1230},"obj":"PRP$"},{"id":"T1364","span":{"begin":1235,"end":1241},"obj":"NN"},{"id":"T1365","span":{"begin":1231,"end":1234},"obj":"NN"},{"id":"T1366","span":{"begin":1242,"end":1245},"obj":"CC"},{"id":"T1367","span":{"begin":1246,"end":1248},"obj":"IN"},{"id":"T1368","span":{"begin":1249,"end":1252},"obj":"DT"},{"id":"T1369","span":{"begin":1264,"end":1270},"obj":"NN"},{"id":"T1370","span":{"begin":1253,"end":1254},"obj":"NN"},{"id":"T1371","span":{"begin":1255,"end":1263},"obj":"JJ"},{"id":"T1372","span":{"begin":1254,"end":1255},"obj":"HYPH"},{"id":"T1373","span":{"begin":1270,"end":1271},"obj":"."}],"relations":[{"id":"R112","pred":"nsubjpass","subj":"T1128","obj":"T1130"},{"id":"R113","pred":"punct","subj":"T1129","obj":"T1128"},{"id":"R123","pred":"relcl","subj":"T1140","obj":"T1138"},{"id":"R124","pred":"pobj","subj":"T1141","obj":"T1139"},{"id":"R125","pred":"det","subj":"T1142","obj":"T1143"},{"id":"R126","pred":"nsubjpass","subj":"T1143","obj":"T1140"},{"id":"R127","pred":"prep","subj":"T1144","obj":"T1143"},{"id":"R128","pred":"compound","subj":"T1145","obj":"T1146"},{"id":"R129","pred":"pobj","subj":"T1146","obj":"T1144"},{"id":"R130","pred":"auxpass","subj":"T1147","obj":"T1140"},{"id":"R131","pred":"advmod","subj":"T1148","obj":"T1140"},{"id":"R132","pred":"advmod","subj":"T1149","obj":"T1140"},{"id":"R137","pred":"ccomp","subj":"T1156","obj":"T1154"},{"id":"R138","pred":"det","subj":"T1157","obj":"T1158"},{"id":"R140","pred":"compound","subj":"T1159","obj":"T1158"},{"id":"R141","pred":"prep","subj":"T1160","obj":"T1158"},{"id":"R142","pred":"compound","subj":"T1161","obj":"T1162"},{"id":"R144","pred":"punct","subj":"T1163","obj":"T1162"},{"id":"R145","pred":"auxpass","subj":"T1164","obj":"T1156"},{"id":"R146","pred":"advmod","subj":"T1165","obj":"T1166"},{"id":"R147","pred":"advmod","subj":"T1166","obj":"T1156"},{"id":"R150","pred":"pobj","subj":"T1169","obj":"T1167"},{"id":"R151","pred":"nmod","subj":"T1170","obj":"T1169"},{"id":"R152","pred":"nmod","subj":"T1171","obj":"T1169"},{"id":"R153","pred":"punct","subj":"T1172","obj":"T1173"},{"id":"R154","pred":"parataxis","subj":"T1173","obj":"T1171"},{"id":"R155","pred":"punct","subj":"T1174","obj":"T1173"},{"id":"R158","pred":"appos","subj":"T1177","obj":"T1169"},{"id":"R159","pred":"punct","subj":"T1178","obj":"T1177"},{"id":"R162","pred":"relcl","subj":"T1181","obj":"T1169"},{"id":"R163","pred":"auxpass","subj":"T1182","obj":"T1181"},{"id":"R166","pred":"pobj","subj":"T1185","obj":"T1183"},{"id":"R167","pred":"amod","subj":"T1186","obj":"T1185"},{"id":"R178","pred":"acl","subj":"T1199","obj":"T1197"},{"id":"R179","pred":"compound","subj":"T1200","obj":"T1201"},{"id":"R181","pred":"punct","subj":"T1202","obj":"T1201"},{"id":"R182","pred":"aux","subj":"T1203","obj":"T1199"},{"id":"R184","pred":"dobj","subj":"T1205","obj":"T1199"},{"id":"R185","pred":"amod","subj":"T1206","obj":"T1205"},{"id":"R195","pred":"nsubjpass","subj":"T1217","obj":"T1219"},{"id":"R196","pred":"punct","subj":"T1218","obj":"T1217"},{"id":"R204","pred":"pobj","subj":"T1227","obj":"T1225"},{"id":"R205","pred":"nmod","subj":"T1228","obj":"T1227"},{"id":"R215","pred":"advcl","subj":"T1238","obj":"T1227"},{"id":"R216","pred":"nsubj","subj":"T1239","obj":"T1238"},{"id":"R223","pred":"pobj","subj":"T1246","obj":"T1244"},{"id":"R224","pred":"amod","subj":"T1247","obj":"T1246"},{"id":"R225","pred":"amod","subj":"T1248","obj":"T1246"},{"id":"R230","pred":"pobj","subj":"T1255","obj":"T1253"},{"id":"R231","pred":"punct","subj":"T1256","obj":"T1255"},{"id":"R236","pred":"pobj","subj":"T1261","obj":"T1259"},{"id":"R237","pred":"compound","subj":"T1262","obj":"T1263"},{"id":"R239","pred":"punct","subj":"T1264","obj":"T1263"},{"id":"R240","pred":"compound","subj":"T1265","obj":"T1261"},{"id":"R246","pred":"ccomp","subj":"T1273","obj":"T1271"},{"id":"R247","pred":"det","subj":"T1274","obj":"T1275"},{"id":"R248","pred":"nsubj","subj":"T1275","obj":"T1273"},{"id":"R249","pred":"compound","subj":"T1276","obj":"T1277"},{"id":"R250","pred":"compound","subj":"T1277","obj":"T1275"},{"id":"R251","pred":"punct","subj":"T1278","obj":"T1277"},{"id":"R252","pred":"dep","subj":"T1279","obj":"T1273"},{"id":"R253","pred":"punct","subj":"T1280","obj":"T1273"},{"id":"R257","pred":"pobj","subj":"T1284","obj":"T1282"},{"id":"R258","pred":"compound","subj":"T1285","obj":"T1286"},{"id":"R259","pred":"npadvmod","subj":"T1286","obj":"T1287"},{"id":"R261","pred":"punct","subj":"T1288","obj":"T1287"},{"id":"R263","pred":"cc","subj":"T1290","obj":"T1284"},{"id":"R264","pred":"advmod","subj":"T1291","obj":"T1290"},{"id":"R268","pred":"nsubj","subj":"T1296","obj":"T1300"},{"id":"R269","pred":"compound","subj":"T1297","obj":"T1298"},{"id":"R271","pred":"punct","subj":"T1299","obj":"T1298"},{"id":"R278","pred":"advcl","subj":"T1307","obj":"T1300"},{"id":"R279","pred":"compound","subj":"T1308","obj":"T1309"},{"id":"R281","pred":"punct","subj":"T1310","obj":"T1309"},{"id":"R282","pred":"auxpass","subj":"T1311","obj":"T1307"},{"id":"R291","pred":"pobj","subj":"T1322","obj":"T1320"},{"id":"R292","pred":"compound","subj":"T1323","obj":"T1322"},{"id":"R296","pred":"ccomp","subj":"T1327","obj":"T1318"},{"id":"R297","pred":"compound","subj":"T1328","obj":"T1329"},{"id":"R299","pred":"punct","subj":"T1330","obj":"T1329"},{"id":"R300","pred":"nsubj","subj":"T1331","obj":"T1327"},{"id":"R301","pred":"acl","subj":"T1332","obj":"T1331"},{"id":"R302","pred":"prep","subj":"T1333","obj":"T1332"},{"id":"R303","pred":"nmod","subj":"T1334","obj":"T1335"},{"id":"R304","pred":"pobj","subj":"T1335","obj":"T1333"},{"id":"R305","pred":"npadvmod","subj":"T1336","obj":"T1337"},{"id":"R306","pred":"amod","subj":"T1337","obj":"T1335"},{"id":"R307","pred":"punct","subj":"T1338","obj":"T1337"},{"id":"R310","pred":"pobj","subj":"T1341","obj":"T1339"},{"id":"R311","pred":"amod","subj":"T1342","obj":"T1341"},{"id":"R315","pred":"ccomp","subj":"T1348","obj":"T1346"},{"id":"R316","pred":"det","subj":"T1349","obj":"T1350"},{"id":"R318","pred":"compound","subj":"T1351","obj":"T1350"},{"id":"R319","pred":"prep","subj":"T1352","obj":"T1350"},{"id":"R320","pred":"compound","subj":"T1353","obj":"T1354"},{"id":"R322","pred":"punct","subj":"T1355","obj":"T1354"},{"id":"R327","pred":"pobj","subj":"T1360","obj":"T1358"},{"id":"R328","pred":"amod","subj":"T1361","obj":"T1360"},{"id":"R331","pred":"pobj","subj":"T1364","obj":"T1362"},{"id":"R332","pred":"compound","subj":"T1365","obj":"T1364"},{"id":"R336","pred":"pobj","subj":"T1369","obj":"T1367"},{"id":"R337","pred":"npadvmod","subj":"T1370","obj":"T1371"},{"id":"R339","pred":"punct","subj":"T1372","obj":"T1371"},{"id":"R111","pred":"compound","subj":"T1127","obj":"T1128"},{"id":"R114","pred":"auxpass","subj":"T1131","obj":"T1130"},{"id":"R115","pred":"advmod","subj":"T1132","obj":"T1130"},{"id":"R116","pred":"prep","subj":"T1133","obj":"T1130"},{"id":"R117","pred":"pobj","subj":"T1134","obj":"T1133"},{"id":"R118","pred":"prep","subj":"T1135","obj":"T1133"},{"id":"R119","pred":"pobj","subj":"T1136","obj":"T1135"},{"id":"R120","pred":"punct","subj":"T1137","obj":"T1130"},{"id":"R121","pred":"npadvmod","subj":"T1138","obj":"T1130"},{"id":"R122","pred":"prep","subj":"T1139","obj":"T1140"},{"id":"R133","pred":"punct","subj":"T1150","obj":"T1130"},{"id":"R134","pred":"amod","subj":"T1152","obj":"T1153"},{"id":"R135","pred":"nsubj","subj":"T1153","obj":"T1154"},{"id":"R136","pred":"mark","subj":"T1155","obj":"T1156"},{"id":"R139","pred":"nsubjpass","subj":"T1158","obj":"T1156"},{"id":"R143","pred":"pobj","subj":"T1162","obj":"T1160"},{"id":"R148","pred":"prep","subj":"T1167","obj":"T1156"},{"id":"R149","pred":"det","subj":"T1168","obj":"T1169"},{"id":"R156","pred":"punct","subj":"T1175","obj":"T1169"},{"id":"R157","pred":"compound","subj":"T1176","obj":"T1177"},{"id":"R160","pred":"punct","subj":"T1179","obj":"T1169"},{"id":"R161","pred":"dep","subj":"T1180","obj":"T1181"},{"id":"R164","pred":"prep","subj":"T1183","obj":"T1181"},{"id":"R165","pred":"det","subj":"T1184","obj":"T1185"},{"id":"R168","pred":"acl","subj":"T1187","obj":"T1185"},{"id":"R169","pred":"prep","subj":"T1188","obj":"T1187"},{"id":"R170","pred":"compound","subj":"T1189","obj":"T1190"},{"id":"R171","pred":"pobj","subj":"T1190","obj":"T1188"},{"id":"R172","pred":"punct","subj":"T1191","obj":"T1154"},{"id":"R173","pred":"det","subj":"T1193","obj":"T1194"},{"id":"R174","pred":"nsubj","subj":"T1194","obj":"T1195"},{"id":"R175","pred":"det","subj":"T1196","obj":"T1197"},{"id":"R176","pred":"dobj","subj":"T1197","obj":"T1195"},{"id":"R177","pred":"mark","subj":"T1198","obj":"T1199"},{"id":"R180","pred":"nsubj","subj":"T1201","obj":"T1199"},{"id":"R183","pred":"det","subj":"T1204","obj":"T1205"},{"id":"R186","pred":"prep","subj":"T1207","obj":"T1199"},{"id":"R187","pred":"pcomp","subj":"T1208","obj":"T1207"},{"id":"R188","pred":"compound","subj":"T1209","obj":"T1210"},{"id":"R189","pred":"dobj","subj":"T1210","obj":"T1208"},{"id":"R190","pred":"prep","subj":"T1211","obj":"T1208"},{"id":"R191","pred":"compound","subj":"T1212","obj":"T1213"},{"id":"R192","pred":"pobj","subj":"T1213","obj":"T1211"},{"id":"R193","pred":"punct","subj":"T1214","obj":"T1195"},{"id":"R194","pred":"compound","subj":"T1216","obj":"T1217"},{"id":"R197","pred":"auxpass","subj":"T1220","obj":"T1219"},{"id":"R198","pred":"advmod","subj":"T1221","obj":"T1219"},{"id":"R199","pred":"prep","subj":"T1222","obj":"T1219"},{"id":"R200","pred":"det","subj":"T1223","obj":"T1224"},{"id":"R201","pred":"pobj","subj":"T1224","obj":"T1222"},{"id":"R202","pred":"prep","subj":"T1225","obj":"T1219"},{"id":"R203","pred":"amod","subj":"T1226","obj":"T1227"},{"id":"R206","pred":"punct","subj":"T1229","obj":"T1230"},{"id":"R207","pred":"prep","subj":"T1230","obj":"T1227"},{"id":"R208","pred":"punct","subj":"T1231","obj":"T1232"},{"id":"R209","pred":"parataxis","subj":"T1232","obj":"T1227"},{"id":"R210","pred":"punct","subj":"T1233","obj":"T1234"},{"id":"R211","pred":"prep","subj":"T1234","obj":"T1232"},{"id":"R212","pred":"punct","subj":"T1235","obj":"T1232"},{"id":"R213","pred":"punct","subj":"T1236","obj":"T1227"},{"id":"R214","pred":"advmod","subj":"T1237","obj":"T1238"},{"id":"R217","pred":"amod","subj":"T1240","obj":"T1241"},{"id":"R218","pred":"dobj","subj":"T1241","obj":"T1238"},{"id":"R219","pred":"punct","subj":"T1242","obj":"T1219"},{"id":"R220","pred":"cc","subj":"T1243","obj":"T1244"},{"id":"R221","pred":"prep","subj":"T1244","obj":"T1219"},{"id":"R222","pred":"det","subj":"T1245","obj":"T1246"},{"id":"R226","pred":"punct","subj":"T1249","obj":"T1219"},{"id":"R227","pred":"nsubjpass","subj":"T1251","obj":"T1252"},{"id":"R228","pred":"prep","subj":"T1253","obj":"T1251"},{"id":"R229","pred":"compound","subj":"T1254","obj":"T1255"},{"id":"R232","pred":"auxpass","subj":"T1257","obj":"T1252"},{"id":"R233","pred":"advmod","subj":"T1258","obj":"T1252"},{"id":"R234","pred":"agent","subj":"T1259","obj":"T1252"},{"id":"R235","pred":"det","subj":"T1260","obj":"T1261"},{"id":"R238","pred":"compound","subj":"T1263","obj":"T1261"},{"id":"R241","pred":"appos","subj":"T1266","obj":"T1261"},{"id":"R242","pred":"punct","subj":"T1267","obj":"T1252"},{"id":"R243","pred":"compound","subj":"T1269","obj":"T1270"},{"id":"R244","pred":"nsubj","subj":"T1270","obj":"T1271"},{"id":"R245","pred":"mark","subj":"T1272","obj":"T1273"},{"id":"R254","pred":"advmod","subj":"T1281","obj":"T1282"},{"id":"R255","pred":"prep","subj":"T1282","obj":"T1273"},{"id":"R256","pred":"det","subj":"T1283","obj":"T1284"},{"id":"R260","pred":"amod","subj":"T1287","obj":"T1284"},{"id":"R262","pred":"advmod","subj":"T1289","obj":"T1290"},{"id":"R265","pred":"conj","subj":"T1292","obj":"T1284"},{"id":"R266","pred":"punct","subj":"T1293","obj":"T1271"},{"id":"R267","pred":"det","subj":"T1295","obj":"T1296"},{"id":"R270","pred":"compound","subj":"T1298","obj":"T1296"},{"id":"R272","pred":"advmod","subj":"T1301","obj":"T1302"},{"id":"R273","pred":"prep","subj":"T1302","obj":"T1300"},{"id":"R274","pred":"det","subj":"T1303","obj":"T1304"},{"id":"R275","pred":"pobj","subj":"T1304","obj":"T1302"},{"id":"R276","pred":"punct","subj":"T1305","obj":"T1300"},{"id":"R277","pred":"advmod","subj":"T1306","obj":"T1307"},{"id":"R280","pred":"nsubjpass","subj":"T1309","obj":"T1307"},{"id":"R283","pred":"prep","subj":"T1312","obj":"T1307"},{"id":"R284","pred":"compound","subj":"T1313","obj":"T1314"},{"id":"R285","pred":"pobj","subj":"T1314","obj":"T1312"},{"id":"R286","pred":"punct","subj":"T1315","obj":"T1300"},{"id":"R287","pred":"advmod","subj":"T1317","obj":"T1318"},{"id":"R288","pred":"punct","subj":"T1319","obj":"T1318"},{"id":"R289","pred":"prep","subj":"T1320","obj":"T1318"},{"id":"R290","pred":"det","subj":"T1321","obj":"T1322"},{"id":"R293","pred":"punct","subj":"T1324","obj":"T1318"},{"id":"R294","pred":"nsubj","subj":"T1325","obj":"T1318"},{"id":"R295","pred":"mark","subj":"T1326","obj":"T1327"},{"id":"R298","pred":"compound","subj":"T1329","obj":"T1331"},{"id":"R308","pred":"prep","subj":"T1339","obj":"T1327"},{"id":"R309","pred":"det","subj":"T1340","obj":"T1341"},{"id":"R312","pred":"punct","subj":"T1343","obj":"T1318"},{"id":"R313","pred":"nsubj","subj":"T1345","obj":"T1346"},{"id":"R314","pred":"mark","subj":"T1347","obj":"T1348"},{"id":"R317","pred":"nsubj","subj":"T1350","obj":"T1348"},{"id":"R321","pred":"pobj","subj":"T1354","obj":"T1352"},{"id":"R323","pred":"neg","subj":"T1356","obj":"T1348"},{"id":"R324","pred":"prep","subj":"T1357","obj":"T1348"},{"id":"R325","pred":"prep","subj":"T1358","obj":"T1357"},{"id":"R326","pred":"det","subj":"T1359","obj":"T1360"},{"id":"R329","pred":"prep","subj":"T1362","obj":"T1360"},{"id":"R330","pred":"poss","subj":"T1363","obj":"T1364"},{"id":"R333","pred":"cc","subj":"T1366","obj":"T1358"},{"id":"R334","pred":"conj","subj":"T1367","obj":"T1358"},{"id":"R335","pred":"det","subj":"T1368","obj":"T1369"},{"id":"R338","pred":"amod","subj":"T1371","obj":"T1369"},{"id":"R340","pred":"punct","subj":"T1373","obj":"T1346"}],"text":"Results\nmr-s is evolutionarily conserved from zebrafish through human, organisms through which the mechanism of photoreceptor development is also highly conserved. Phylogenetic analysis suggests that the SAM domain of mr-s is most closely related to a mouse polyhomeotic (ph) ortholog, Mph1/Rae28, which is known as an epigenetic molecule involved in chromatin modifications. These findings provide the possibility that mr-s may play a critical role by regulating gene expression in photoreceptor development. mr-s is preferentially expressed in the photoreceptors at postnatal day 3–6 (P3-6), when photoreceptors undergo terminal differentiation, and in the adult pineal gland. Transcription of mr-s is directly regulated by the cone-rod homeodomain protein Crx. Immunoprecipitation assay showed that the mr-s protein self-associates mainly through the SAM domain-containing region as well as ph. The mr-s protein localizes mainly in the nucleus, when mr-s is overexpressed in HEK293T cells. Moreover, in the luciferase assays, we found that mr-s protein fused to GAL4 DNA-binding domain functions as a transcriptional repressor. We revealed that the repression activity of mr-s is not due to a homophilic interaction through its SAM domain but to the C-terminal region."}