PMC:1435744 / 17623-18265 JSONTXT

Annnotations TAB JSON ListView MergeView

    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T9690","span":{"begin":29,"end":36},"obj":"SO_EXT:0000440"},{"id":"T9691","span":{"begin":46,"end":55},"obj":"SO_EXT:sequence_alteration_entity_or_process"},{"id":"T9692","span":{"begin":85,"end":88},"obj":"PR_EXT:000005904"},{"id":"T9693","span":{"begin":89,"end":96},"obj":"CHEMINF_GO_EXT:chemical_binding_or_bond_formation"},{"id":"T9694","span":{"begin":89,"end":102},"obj":"SO_EXT:0000409"},{"id":"T9695","span":{"begin":137,"end":140},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T9696","span":{"begin":156,"end":159},"obj":"PR_EXT:000005904"},{"id":"T9697","span":{"begin":160,"end":170},"obj":"GO:0010467"},{"id":"T9698","span":{"begin":171,"end":177},"obj":"SO_EXT:0000440"},{"id":"T9699","span":{"begin":228,"end":231},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T9700","span":{"begin":292,"end":295},"obj":"PR_EXT:000005904"},{"id":"T9701","span":{"begin":395,"end":406},"obj":"GO:0009294"},{"id":"T9702","span":{"begin":440,"end":443},"obj":"PR_EXT:000005904"},{"id":"T9703","span":{"begin":492,"end":495},"obj":"PR_EXT:000005904"},{"id":"T9704","span":{"begin":496,"end":503},"obj":"CHEMINF_GO_EXT:chemical_binding_or_bond_formation"},{"id":"T9705","span":{"begin":496,"end":509},"obj":"SO_EXT:0000409"},{"id":"T9706","span":{"begin":513,"end":515},"obj":"SO_EXT:0000028"},{"id":"T9707","span":{"begin":524,"end":526},"obj":"SO_EXT:0000028"},{"id":"T9708","span":{"begin":527,"end":535},"obj":"SO_EXT:sequence_upstreamness"},{"id":"T9709","span":{"begin":545,"end":558},"obj":"GO_EXT:transcription"},{"id":"T9710","span":{"begin":545,"end":574},"obj":"SO_EXT:0000315"},{"id":"T9711","span":{"begin":602,"end":612},"obj":"GO:0065007"},{"id":"T9712","span":{"begin":616,"end":620},"obj":"PR_EXT:000014441"},{"id":"T9713","span":{"begin":621,"end":634},"obj":"GO_EXT:transcription"},{"id":"T9714","span":{"begin":638,"end":641},"obj":"PR_EXT:000005904"}],"text":"We also constructed reporter vectors in which mutations were introduced at the three Crx binding sites (Fig. 3D, mut1259, mut198, mut72, mut all). Then the Crx expression vector was co-introduced with mut1259, mut198, mut72 and mut all, respectively (Fig. 3F). The transactivaton activity by Crx was clearly reduced when mut198 or mut72 was co-introduced. On the other hand, when mut1259 was co-transfected, the transactivation activity by Crx was not altered. These results suggest that the Crx binding sites 72-bp and 198-bp upstream from the transcription initiation site are crucial for the direct regulation of mr-s transcription by Crx."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T9515","span":{"begin":29,"end":36},"obj":"SO:0000440"},{"id":"T9516","span":{"begin":85,"end":88},"obj":"PR:000005904"},{"id":"T9517","span":{"begin":89,"end":102},"obj":"SO:0000409"},{"id":"T9518","span":{"begin":156,"end":159},"obj":"PR:000005904"},{"id":"T9519","span":{"begin":160,"end":170},"obj":"GO:0010467"},{"id":"T9520","span":{"begin":171,"end":177},"obj":"SO:0000440"},{"id":"T9521","span":{"begin":292,"end":295},"obj":"PR:000005904"},{"id":"T9522","span":{"begin":395,"end":406},"obj":"GO:0009294"},{"id":"T9523","span":{"begin":440,"end":443},"obj":"PR:000005904"},{"id":"T9524","span":{"begin":492,"end":495},"obj":"PR:000005904"},{"id":"T9525","span":{"begin":496,"end":509},"obj":"SO:0000409"},{"id":"T9526","span":{"begin":513,"end":515},"obj":"SO:0000028"},{"id":"T9527","span":{"begin":524,"end":526},"obj":"SO:0000028"},{"id":"T9528","span":{"begin":545,"end":574},"obj":"SO:0000315"},{"id":"T9529","span":{"begin":602,"end":612},"obj":"GO:0065007"},{"id":"T9530","span":{"begin":616,"end":620},"obj":"PR:000014441"},{"id":"T9531","span":{"begin":638,"end":641},"obj":"PR:000005904"}],"text":"We also constructed reporter vectors in which mutations were introduced at the three Crx binding sites (Fig. 3D, mut1259, mut198, mut72, mut all). Then the Crx expression vector was co-introduced with mut1259, mut198, mut72 and mut all, respectively (Fig. 3F). The transactivaton activity by Crx was clearly reduced when mut198 or mut72 was co-introduced. On the other hand, when mut1259 was co-transfected, the transactivation activity by Crx was not altered. These results suggest that the Crx binding sites 72-bp and 198-bp upstream from the transcription initiation site are crucial for the direct regulation of mr-s transcription by Crx."}

    craft-sa-dev

    {"project":"craft-sa-dev","denotations":[{"id":"T10302","span":{"begin":0,"end":2},"obj":"PRP"},{"id":"T10303","span":{"begin":8,"end":19},"obj":"VBD"},{"id":"T10304","span":{"begin":3,"end":7},"obj":"RB"},{"id":"T10305","span":{"begin":20,"end":28},"obj":"NN"},{"id":"T10306","span":{"begin":29,"end":36},"obj":"NNS"},{"id":"T10307","span":{"begin":37,"end":39},"obj":"IN"},{"id":"T10308","span":{"begin":61,"end":71},"obj":"VBN"},{"id":"T10309","span":{"begin":40,"end":45},"obj":"WDT"},{"id":"T10310","span":{"begin":46,"end":55},"obj":"NNS"},{"id":"T10311","span":{"begin":56,"end":60},"obj":"VBD"},{"id":"T10312","span":{"begin":72,"end":74},"obj":"IN"},{"id":"T10313","span":{"begin":75,"end":78},"obj":"DT"},{"id":"T10314","span":{"begin":97,"end":102},"obj":"NNS"},{"id":"T10315","span":{"begin":79,"end":84},"obj":"CD"},{"id":"T10316","span":{"begin":85,"end":88},"obj":"NN"},{"id":"T10317","span":{"begin":89,"end":96},"obj":"NN"},{"id":"T10318","span":{"begin":103,"end":104},"obj":"-LRB-"},{"id":"T10319","span":{"begin":113,"end":120},"obj":"NN"},{"id":"T10320","span":{"begin":104,"end":108},"obj":"NN"},{"id":"T10321","span":{"begin":109,"end":111},"obj":"NN"},{"id":"T10322","span":{"begin":111,"end":113},"obj":","},{"id":"T10323","span":{"begin":120,"end":122},"obj":","},{"id":"T10324","span":{"begin":122,"end":128},"obj":"NN"},{"id":"T10325","span":{"begin":128,"end":130},"obj":","},{"id":"T10326","span":{"begin":130,"end":135},"obj":"NN"},{"id":"T10327","span":{"begin":135,"end":137},"obj":","},{"id":"T10328","span":{"begin":137,"end":140},"obj":"NN"},{"id":"T10329","span":{"begin":141,"end":144},"obj":"NN"},{"id":"T10330","span":{"begin":144,"end":145},"obj":"-RRB-"},{"id":"T10331","span":{"begin":145,"end":146},"obj":"."},{"id":"T10332","span":{"begin":146,"end":260},"obj":"sentence"},{"id":"T10333","span":{"begin":147,"end":151},"obj":"RB"},{"id":"T10334","span":{"begin":182,"end":195},"obj":"VBN"},{"id":"T10335","span":{"begin":152,"end":155},"obj":"DT"},{"id":"T10336","span":{"begin":171,"end":177},"obj":"NN"},{"id":"T10337","span":{"begin":156,"end":159},"obj":"NN"},{"id":"T10338","span":{"begin":160,"end":170},"obj":"NN"},{"id":"T10339","span":{"begin":178,"end":181},"obj":"VBD"},{"id":"T10340","span":{"begin":196,"end":200},"obj":"IN"},{"id":"T10341","span":{"begin":201,"end":208},"obj":"NN"},{"id":"T10342","span":{"begin":208,"end":210},"obj":","},{"id":"T10343","span":{"begin":210,"end":216},"obj":"NN"},{"id":"T10344","span":{"begin":216,"end":218},"obj":","},{"id":"T10345","span":{"begin":218,"end":223},"obj":"NN"},{"id":"T10346","span":{"begin":224,"end":227},"obj":"CC"},{"id":"T10347","span":{"begin":228,"end":231},"obj":"NN"},{"id":"T10348","span":{"begin":232,"end":235},"obj":"NN"},{"id":"T10349","span":{"begin":235,"end":237},"obj":","},{"id":"T10350","span":{"begin":237,"end":249},"obj":"RB"},{"id":"T10351","span":{"begin":250,"end":251},"obj":"-LRB-"},{"id":"T10352","span":{"begin":256,"end":258},"obj":"NN"},{"id":"T10353","span":{"begin":251,"end":255},"obj":"NN"},{"id":"T10354","span":{"begin":258,"end":259},"obj":"-RRB-"},{"id":"T10355","span":{"begin":259,"end":260},"obj":"."},{"id":"T10356","span":{"begin":260,"end":355},"obj":"sentence"},{"id":"T10357","span":{"begin":261,"end":264},"obj":"DT"},{"id":"T10358","span":{"begin":280,"end":288},"obj":"NN"},{"id":"T10359","span":{"begin":265,"end":279},"obj":"NN"},{"id":"T10360","span":{"begin":308,"end":315},"obj":"VBN"},{"id":"T10361","span":{"begin":289,"end":291},"obj":"IN"},{"id":"T10362","span":{"begin":292,"end":295},"obj":"NN"},{"id":"T10363","span":{"begin":296,"end":299},"obj":"VBD"},{"id":"T10364","span":{"begin":300,"end":307},"obj":"RB"},{"id":"T10365","span":{"begin":316,"end":320},"obj":"WRB"},{"id":"T10366","span":{"begin":341,"end":354},"obj":"VBN"},{"id":"T10367","span":{"begin":321,"end":327},"obj":"NN"},{"id":"T10368","span":{"begin":328,"end":330},"obj":"CC"},{"id":"T10369","span":{"begin":331,"end":336},"obj":"NN"},{"id":"T10370","span":{"begin":337,"end":340},"obj":"VBD"},{"id":"T10371","span":{"begin":354,"end":355},"obj":"."},{"id":"T10372","span":{"begin":355,"end":460},"obj":"sentence"},{"id":"T10373","span":{"begin":356,"end":358},"obj":"IN"},{"id":"T10374","span":{"begin":452,"end":459},"obj":"VBN"},{"id":"T10375","span":{"begin":359,"end":362},"obj":"DT"},{"id":"T10376","span":{"begin":369,"end":373},"obj":"NN"},{"id":"T10377","span":{"begin":363,"end":368},"obj":"JJ"},{"id":"T10378","span":{"begin":373,"end":375},"obj":","},{"id":"T10379","span":{"begin":375,"end":379},"obj":"WRB"},{"id":"T10380","span":{"begin":392,"end":406},"obj":"VBN"},{"id":"T10381","span":{"begin":380,"end":387},"obj":"NN"},{"id":"T10382","span":{"begin":388,"end":391},"obj":"VBD"},{"id":"T10383","span":{"begin":406,"end":408},"obj":","},{"id":"T10384","span":{"begin":408,"end":411},"obj":"DT"},{"id":"T10385","span":{"begin":428,"end":436},"obj":"NN"},{"id":"T10386","span":{"begin":412,"end":427},"obj":"NN"},{"id":"T10387","span":{"begin":437,"end":439},"obj":"IN"},{"id":"T10388","span":{"begin":440,"end":443},"obj":"NN"},{"id":"T10389","span":{"begin":444,"end":447},"obj":"VBD"},{"id":"T10390","span":{"begin":448,"end":451},"obj":"RB"},{"id":"T10391","span":{"begin":459,"end":460},"obj":"."},{"id":"T10392","span":{"begin":460,"end":642},"obj":"sentence"},{"id":"T10393","span":{"begin":461,"end":466},"obj":"DT"},{"id":"T10394","span":{"begin":467,"end":474},"obj":"NNS"},{"id":"T10395","span":{"begin":475,"end":482},"obj":"VBP"},{"id":"T10396","span":{"begin":483,"end":487},"obj":"IN"},{"id":"T10397","span":{"begin":575,"end":578},"obj":"VBP"},{"id":"T10398","span":{"begin":488,"end":491},"obj":"DT"},{"id":"T10399","span":{"begin":504,"end":509},"obj":"NNS"},{"id":"T10400","span":{"begin":492,"end":495},"obj":"NN"},{"id":"T10401","span":{"begin":496,"end":503},"obj":"NN"},{"id":"T10402","span":{"begin":510,"end":512},"obj":"CD"},{"id":"T10403","span":{"begin":513,"end":515},"obj":"NN"},{"id":"T10404","span":{"begin":512,"end":513},"obj":"HYPH"},{"id":"T10405","span":{"begin":527,"end":535},"obj":"RB"},{"id":"T10406","span":{"begin":516,"end":519},"obj":"CC"},{"id":"T10407","span":{"begin":520,"end":523},"obj":"CD"},{"id":"T10408","span":{"begin":524,"end":526},"obj":"NN"},{"id":"T10409","span":{"begin":523,"end":524},"obj":"HYPH"},{"id":"T10410","span":{"begin":536,"end":540},"obj":"IN"},{"id":"T10411","span":{"begin":541,"end":544},"obj":"DT"},{"id":"T10412","span":{"begin":570,"end":574},"obj":"NN"},{"id":"T10413","span":{"begin":545,"end":558},"obj":"NN"},{"id":"T10414","span":{"begin":559,"end":569},"obj":"NN"},{"id":"T10415","span":{"begin":579,"end":586},"obj":"JJ"},{"id":"T10416","span":{"begin":587,"end":590},"obj":"IN"},{"id":"T10417","span":{"begin":591,"end":594},"obj":"DT"},{"id":"T10418","span":{"begin":602,"end":612},"obj":"NN"},{"id":"T10419","span":{"begin":595,"end":601},"obj":"JJ"},{"id":"T10420","span":{"begin":613,"end":615},"obj":"IN"},{"id":"T10421","span":{"begin":616,"end":618},"obj":"NN"},{"id":"T10422","span":{"begin":619,"end":620},"obj":"NN"},{"id":"T10423","span":{"begin":618,"end":619},"obj":"HYPH"},{"id":"T10424","span":{"begin":621,"end":634},"obj":"NN"},{"id":"T10425","span":{"begin":635,"end":637},"obj":"IN"},{"id":"T10426","span":{"begin":638,"end":641},"obj":"NN"},{"id":"T10427","span":{"begin":641,"end":642},"obj":"."}],"relations":[{"id":"R2750","pred":"nsubj","subj":"T10302","obj":"T10303"},{"id":"R2751","pred":"advmod","subj":"T10304","obj":"T10303"},{"id":"R2752","pred":"compound","subj":"T10305","obj":"T10306"},{"id":"R2753","pred":"dobj","subj":"T10306","obj":"T10303"},{"id":"R2754","pred":"prep","subj":"T10307","obj":"T10308"},{"id":"R2755","pred":"relcl","subj":"T10308","obj":"T10306"},{"id":"R2756","pred":"pobj","subj":"T10309","obj":"T10307"},{"id":"R2757","pred":"nsubjpass","subj":"T10310","obj":"T10308"},{"id":"R2758","pred":"auxpass","subj":"T10311","obj":"T10308"},{"id":"R2759","pred":"prep","subj":"T10312","obj":"T10308"},{"id":"R2760","pred":"det","subj":"T10313","obj":"T10314"},{"id":"R2761","pred":"pobj","subj":"T10314","obj":"T10312"},{"id":"R2762","pred":"nummod","subj":"T10315","obj":"T10314"},{"id":"R2763","pred":"compound","subj":"T10316","obj":"T10317"},{"id":"R2764","pred":"compound","subj":"T10317","obj":"T10314"},{"id":"R2765","pred":"punct","subj":"T10318","obj":"T10319"},{"id":"R2766","pred":"parataxis","subj":"T10319","obj":"T10303"},{"id":"R2767","pred":"compound","subj":"T10320","obj":"T10321"},{"id":"R2768","pred":"dep","subj":"T10321","obj":"T10319"},{"id":"R2769","pred":"punct","subj":"T10322","obj":"T10319"},{"id":"R2770","pred":"punct","subj":"T10323","obj":"T10319"},{"id":"R2771","pred":"appos","subj":"T10324","obj":"T10319"},{"id":"R2772","pred":"punct","subj":"T10325","obj":"T10319"},{"id":"R2773","pred":"appos","subj":"T10326","obj":"T10319"},{"id":"R2774","pred":"punct","subj":"T10327","obj":"T10319"},{"id":"R2775","pred":"compound","subj":"T10328","obj":"T10329"},{"id":"R2776","pred":"appos","subj":"T10329","obj":"T10319"},{"id":"R2777","pred":"punct","subj":"T10330","obj":"T10319"},{"id":"R2778","pred":"punct","subj":"T10331","obj":"T10303"},{"id":"R2779","pred":"advmod","subj":"T10333","obj":"T10334"},{"id":"R2780","pred":"det","subj":"T10335","obj":"T10336"},{"id":"R2781","pred":"nsubjpass","subj":"T10336","obj":"T10334"},{"id":"R2782","pred":"compound","subj":"T10337","obj":"T10336"},{"id":"R2783","pred":"compound","subj":"T10338","obj":"T10336"},{"id":"R2784","pred":"auxpass","subj":"T10339","obj":"T10334"},{"id":"R2785","pred":"prep","subj":"T10340","obj":"T10334"},{"id":"R2786","pred":"pobj","subj":"T10341","obj":"T10340"},{"id":"R2787","pred":"punct","subj":"T10342","obj":"T10341"},{"id":"R2788","pred":"conj","subj":"T10343","obj":"T10341"},{"id":"R2789","pred":"punct","subj":"T10344","obj":"T10343"},{"id":"R2790","pred":"conj","subj":"T10345","obj":"T10343"},{"id":"R2791","pred":"cc","subj":"T10346","obj":"T10345"},{"id":"R2792","pred":"compound","subj":"T10347","obj":"T10348"},{"id":"R2793","pred":"conj","subj":"T10348","obj":"T10345"},{"id":"R2794","pred":"punct","subj":"T10349","obj":"T10334"},{"id":"R2795","pred":"advmod","subj":"T10350","obj":"T10334"},{"id":"R2796","pred":"punct","subj":"T10351","obj":"T10352"},{"id":"R2797","pred":"parataxis","subj":"T10352","obj":"T10334"},{"id":"R2798","pred":"compound","subj":"T10353","obj":"T10352"},{"id":"R2799","pred":"punct","subj":"T10354","obj":"T10352"},{"id":"R2800","pred":"punct","subj":"T10355","obj":"T10334"},{"id":"R2801","pred":"det","subj":"T10357","obj":"T10358"},{"id":"R2802","pred":"nsubjpass","subj":"T10358","obj":"T10360"},{"id":"R2803","pred":"compound","subj":"T10359","obj":"T10358"},{"id":"R2804","pred":"prep","subj":"T10361","obj":"T10358"},{"id":"R2805","pred":"pobj","subj":"T10362","obj":"T10361"},{"id":"R2806","pred":"auxpass","subj":"T10363","obj":"T10360"},{"id":"R2807","pred":"advmod","subj":"T10364","obj":"T10360"},{"id":"R2808","pred":"advmod","subj":"T10365","obj":"T10366"},{"id":"R2809","pred":"advcl","subj":"T10366","obj":"T10360"},{"id":"R2810","pred":"nsubjpass","subj":"T10367","obj":"T10366"},{"id":"R2811","pred":"cc","subj":"T10368","obj":"T10367"},{"id":"R2812","pred":"conj","subj":"T10369","obj":"T10367"},{"id":"R2813","pred":"auxpass","subj":"T10370","obj":"T10366"},{"id":"R2814","pred":"punct","subj":"T10371","obj":"T10360"},{"id":"R2815","pred":"prep","subj":"T10373","obj":"T10374"},{"id":"R2816","pred":"det","subj":"T10375","obj":"T10376"},{"id":"R2817","pred":"pobj","subj":"T10376","obj":"T10373"},{"id":"R2818","pred":"amod","subj":"T10377","obj":"T10376"},{"id":"R2819","pred":"punct","subj":"T10378","obj":"T10374"},{"id":"R2820","pred":"advmod","subj":"T10379","obj":"T10380"},{"id":"R2821","pred":"advcl","subj":"T10380","obj":"T10374"},{"id":"R2822","pred":"nsubjpass","subj":"T10381","obj":"T10380"},{"id":"R2823","pred":"auxpass","subj":"T10382","obj":"T10380"},{"id":"R2824","pred":"punct","subj":"T10383","obj":"T10374"},{"id":"R2825","pred":"det","subj":"T10384","obj":"T10385"},{"id":"R2826","pred":"nsubjpass","subj":"T10385","obj":"T10374"},{"id":"R2827","pred":"compound","subj":"T10386","obj":"T10385"},{"id":"R2828","pred":"prep","subj":"T10387","obj":"T10385"},{"id":"R2829","pred":"pobj","subj":"T10388","obj":"T10387"},{"id":"R2830","pred":"auxpass","subj":"T10389","obj":"T10374"},{"id":"R2831","pred":"neg","subj":"T10390","obj":"T10374"},{"id":"R2832","pred":"punct","subj":"T10391","obj":"T10374"},{"id":"R2833","pred":"det","subj":"T10393","obj":"T10394"},{"id":"R2834","pred":"nsubj","subj":"T10394","obj":"T10395"},{"id":"R2835","pred":"mark","subj":"T10396","obj":"T10397"},{"id":"R2836","pred":"ccomp","subj":"T10397","obj":"T10395"},{"id":"R2837","pred":"det","subj":"T10398","obj":"T10399"},{"id":"R2838","pred":"nsubj","subj":"T10399","obj":"T10397"},{"id":"R2839","pred":"compound","subj":"T10400","obj":"T10401"},{"id":"R2840","pred":"compound","subj":"T10401","obj":"T10399"},{"id":"R2841","pred":"nummod","subj":"T10402","obj":"T10403"},{"id":"R2842","pred":"npadvmod","subj":"T10403","obj":"T10405"},{"id":"R2843","pred":"punct","subj":"T10404","obj":"T10403"},{"id":"R2844","pred":"advmod","subj":"T10405","obj":"T10399"},{"id":"R2845","pred":"cc","subj":"T10406","obj":"T10403"},{"id":"R2846","pred":"nummod","subj":"T10407","obj":"T10408"},{"id":"R2847","pred":"conj","subj":"T10408","obj":"T10403"},{"id":"R2848","pred":"punct","subj":"T10409","obj":"T10408"},{"id":"R2849","pred":"prep","subj":"T10410","obj":"T10405"},{"id":"R2850","pred":"det","subj":"T10411","obj":"T10412"},{"id":"R2851","pred":"pobj","subj":"T10412","obj":"T10410"},{"id":"R2852","pred":"compound","subj":"T10413","obj":"T10412"},{"id":"R2853","pred":"compound","subj":"T10414","obj":"T10412"},{"id":"R2854","pred":"acomp","subj":"T10415","obj":"T10397"},{"id":"R2855","pred":"prep","subj":"T10416","obj":"T10415"},{"id":"R2856","pred":"det","subj":"T10417","obj":"T10418"},{"id":"R2857","pred":"pobj","subj":"T10418","obj":"T10416"},{"id":"R2858","pred":"amod","subj":"T10419","obj":"T10418"},{"id":"R2859","pred":"prep","subj":"T10420","obj":"T10418"},{"id":"R2860","pred":"compound","subj":"T10421","obj":"T10422"},{"id":"R2861","pred":"compound","subj":"T10422","obj":"T10424"},{"id":"R2862","pred":"punct","subj":"T10423","obj":"T10422"},{"id":"R2863","pred":"pobj","subj":"T10424","obj":"T10420"},{"id":"R2864","pred":"prep","subj":"T10425","obj":"T10418"},{"id":"R2865","pred":"pobj","subj":"T10426","obj":"T10425"},{"id":"R2866","pred":"punct","subj":"T10427","obj":"T10395"}],"text":"We also constructed reporter vectors in which mutations were introduced at the three Crx binding sites (Fig. 3D, mut1259, mut198, mut72, mut all). Then the Crx expression vector was co-introduced with mut1259, mut198, mut72 and mut all, respectively (Fig. 3F). The transactivaton activity by Crx was clearly reduced when mut198 or mut72 was co-introduced. On the other hand, when mut1259 was co-transfected, the transactivation activity by Crx was not altered. These results suggest that the Crx binding sites 72-bp and 198-bp upstream from the transcription initiation site are crucial for the direct regulation of mr-s transcription by Crx."}