PMC:1420271 / 5868-6067 JSONTXT

{"project":"craft-ca-core-dev","denotations":[{"id":"T3826","span":{"begin":40,"end":44},"obj":"PR:000006847"},{"id":"T3827","span":{"begin":45,"end":49},"obj":"SO:0000704"},{"id":"T3828","span":{"begin":67,"end":76},"obj":"NCBITaxon:2"},{"id":"T3829","span":{"begin":67,"end":98},"obj":"SO:0000153"},{"id":"T3830","span":{"begin":100,"end":103},"obj":"SO:0000153"},{"id":"T3831","span":{"begin":127,"end":133},"obj":"SO:0000112"},{"id":"T3832","span":{"begin":173,"end":177},"obj":"SO:0000704"},{"id":"T3833","span":{"begin":187,"end":198},"obj":"SO:0000336"}],"text":"\nTo obtain DNA fragments containing the ESG1 gene, we screened the bacterial artificial chromosome (BAC) DNA pool by PCR using primer pairs that would only amplify the real gene, not the pseudogenes."}

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