PMC:1420271 / 10268-10733 JSONTXT

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    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T6141","span":{"begin":28,"end":36},"obj":"CL:0002322"},{"id":"T6142","span":{"begin":62,"end":68},"obj":"SO:0000440"},{"id":"T6143","span":{"begin":92,"end":100},"obj":"CL:0002322"},{"id":"T6144","span":{"begin":109,"end":113},"obj":"CHEBI:42768"},{"id":"T6145","span":{"begin":164,"end":168},"obj":"PR:000006847"},{"id":"T6146","span":{"begin":244,"end":248},"obj":"PR:000006847"},{"id":"T6147","span":{"begin":301,"end":307},"obj":"SO:0000440"},{"id":"T6148","span":{"begin":330,"end":336},"obj":"SO:0000440"},{"id":"T6149","span":{"begin":419,"end":425},"obj":"SO:0000440"}],"text":"To obtain homozygous mutant ES cells, we introduced the β-geo vector into HygR heterozygous ES cells. Of 105 G418-resistant colonies tested, 49 were homozygous for ESG1 deletion. Northern blot and western blot analyses confirmed the absence of ESG1 in these cells (Figure 4C). In 29 clones, the β-geo vector had replaced the HygR vector, such that the cells remained heterozygous. In the remaining 27 clones, the β-geo vector was integrated at non-homologous sites."}

    craft-ca-core-ex-dev

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    craft-sa-dev

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obtain homozygous mutant ES cells, we introduced the β-geo vector into HygR heterozygous ES cells. Of 105 G418-resistant colonies tested, 49 were homozygous for ESG1 deletion. Northern blot and western blot analyses confirmed the absence of ESG1 in these cells (Figure 4C). In 29 clones, the β-geo vector had replaced the HygR vector, such that the cells remained heterozygous. In the remaining 27 clones, the β-geo vector was integrated at non-homologous sites."}