PMC:13916 / 14954-15622 JSONTXT

{"project":"Colil","denotations":[{"id":"T28","span":{"begin":66,"end":68},"obj":"2910432"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/"}],"text":"Cells and cell culture\nThe estrogen receptor-positive, BG-1 line [13], which contains an abundant amount of estrogen receptors (600 fmol/100 μg DNA), was provided by J Boyd (Sloan-Kettering Cancer Center, New York, NY, USA). GPE86 and PA317 viral packaging cell lines were provided by AD Miller (Fred Hutchinson Cancer Center, Seattle, WA, USA). BG-1 cells were maintained in Dulbecco's modified eagle medium (DMEM)/F12 medium supplemented with 10% fetal calf serum (Summit, Fort Collins, CO, USA), and 50 units/ml penicillin/streptomycin. BG-1 cells arrest to gamma radiation consistent with a wildtype p53 phenotype. These cells were tested negative for mycoplasmas."}