PMC:13902 / 19270-20265 JSONTXT

{"project":"Colil","denotations":[{"id":"T55","span":{"begin":991,"end":993},"obj":"2350783"},{"id":"T56","span":{"begin":586,"end":588},"obj":"2465297"},{"id":"T57","span":{"begin":910,"end":912},"obj":"1764993"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/"}],"text":"Immunohistochemical staining was done using rabbit polyclonal antibodies raised against synthetic peptides that correspond to regions in the mature forms of TGF-β1, TGF-β2, and TGF-β3. Antibodies to TGF-β1 were raised against a synthetic peptide corresponding to residues 1-30 of the mature protein in either the Laboratory of Chemoprevention (anti-TGF-β1-LC; NIH, Bethesda, MD, USA) or the Collagen Corporation (anti-TGF-β1-CC; Palo Alto, CA, USA). These antibodies were raised against different preparations of the 1-30 peptide, and they recognize different epitopes of this peptide [19]. The LC antibody usually stains intracellular TGF-β1, whereas the CC antibody stains extracellular TGF-β1. Anti-TGF-β2 (sc-90; Santa Cruz Biotechnologies Inc) was raised to a peptide corresponding to residues 72-99 of the mature TGF-β2. Anti-TGF-β3 (anti-50-60-β3-LC) was raised against residues 50-60 of mature TGF-β3 [20]. Anti-LTBP (Ab39) was raised against the purified full-length platelet LTBP [21]."}