PMC:1382200 / 39398-39615 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1382200","sourcedb":"PMC","sourceid":"1382200","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1382200","text":" Isolated embryos were then digested for five hours at 55°C in 20 μL of lysis buffer (50 mM Tris-HCl, 0.5% Triton X-100, 200 μL/mL proteinase K, pH 8.0), followed by ten minutes at 95°C to inactivate the proteinase K.","tracks":[{"project":"craft-sa-dev","denotations":[{"id":"T20576","span":{"begin":119,"end":121},"obj":","},{"id":"T20575","span":{"begin":116,"end":119},"obj":"CD"},{"id":"T20574","span":{"begin":115,"end":116},"obj":"HYPH"},{"id":"T20573","span":{"begin":107,"end":113},"obj":"NNP"},{"id":"T20572","span":{"begin":114,"end":115},"obj":"NN"},{"id":"T20571","span":{"begin":105,"end":106},"obj":"NN"},{"id":"T20570","span":{"begin":102,"end":105},"obj":"CD"},{"id":"T20569","span":{"begin":100,"end":102},"obj":","},{"id":"T20568","span":{"begin":96,"end":97},"obj":"HYPH"},{"id":"T20567","span":{"begin":92,"end":96},"obj":"NN"},{"id":"T20566","span":{"begin":97,"end":100},"obj":"NN"},{"id":"T20565","span":{"begin":89,"end":91},"obj":"NN"},{"id":"T20564","span":{"begin":86,"end":88},"obj":"CD"},{"id":"T20563","span":{"begin":145,"end":147},"obj":"NN"},{"id":"T20562","span":{"begin":85,"end":86},"obj":"-LRB-"},{"id":"T20561","span":{"begin":78,"end":84},"obj":"NN"},{"id":"T20560","span":{"begin":72,"end":77},"obj":"NN"},{"id":"T20559","span":{"begin":69,"end":71},"obj":"IN"},{"id":"T20558","span":{"begin":66,"end":68},"obj":"NN"},{"id":"T20557","span":{"begin":63,"end":65},"obj":"CD"},{"id":"T20556","span":{"begin":60,"end":62},"obj":"IN"},{"id":"T20555","span":{"begin":57,"end":59},"obj":"NN"},{"id":"T20554","span":{"begin":55,"end":57},"obj":"CD"},{"id":"T20553","span":{"begin":52,"end":54},"obj":"IN"},{"id":"T20552","span":{"begin":46,"end":51},"obj":"NNS"},{"id":"T20551","span":{"begin":41,"end":45},"obj":"CD"},{"id":"T20550","span":{"begin":37,"end":40},"obj":"IN"},{"id":"T20549","span":{"begin":23,"end":27},"obj":"RB"},{"id":"T20548","span":{"begin":18,"end":22},"obj":"VBD"},{"id":"T20547","span":{"begin":28,"end":36},"obj":"VBN"},{"id":"T20546","span":{"begin":10,"end":17},"obj":"NNS"},{"id":"T20545","span":{"begin":1,"end":9},"obj":"VBN"},{"id":"T20544","span":{"begin":0,"end":217},"obj":"sentence"},{"id":"T20599","span":{"begin":216,"end":217},"obj":"."},{"id":"T20598","span":{"begin":204,"end":214},"obj":"NN"},{"id":"T20597","span":{"begin":215,"end":216},"obj":"NN"},{"id":"T20596","span":{"begin":200,"end":203},"obj":"DT"},{"id":"T20595","span":{"begin":189,"end":199},"obj":"VB"},{"id":"T20594","span":{"begin":186,"end":188},"obj":"TO"},{"id":"T20593","span":{"begin":183,"end":185},"obj":"NN"},{"id":"T20592","span":{"begin":181,"end":183},"obj":"CD"},{"id":"T20591","span":{"begin":178,"end":180},"obj":"IN"},{"id":"T20590","span":{"begin":170,"end":177},"obj":"NNS"},{"id":"T20589","span":{"begin":166,"end":169},"obj":"CD"},{"id":"T20588","span":{"begin":163,"end":165},"obj":"IN"},{"id":"T20587","span":{"begin":154,"end":162},"obj":"VBN"},{"id":"T20586","span":{"begin":152,"end":154},"obj":","},{"id":"T20585","span":{"begin":151,"end":152},"obj":"-RRB-"},{"id":"T20584","span":{"begin":148,"end":151},"obj":"CD"},{"id":"T20583","span":{"begin":143,"end":145},"obj":","},{"id":"T20582","span":{"begin":131,"end":141},"obj":"NN"},{"id":"T20581","span":{"begin":128,"end":130},"obj":"NN"},{"id":"T20580","span":{"begin":127,"end":128},"obj":"SYM"},{"id":"T20579","span":{"begin":142,"end":143},"obj":"NN"},{"id":"T20578","span":{"begin":125,"end":127},"obj":"NN"},{"id":"T20577","span":{"begin":121,"end":124},"obj":"CD"}],"relations":[{"id":"R6171","pred":"amod","subj":"T20545","obj":"T20546"},{"id":"R6172","pred":"nsubjpass","subj":"T20546","obj":"T20547"},{"id":"R6173","pred":"auxpass","subj":"T20548","obj":"T20547"},{"id":"R6174","pred":"advmod","subj":"T20549","obj":"T20547"},{"id":"R6175","pred":"prep","subj":"T20550","obj":"T20547"},{"id":"R6176","pred":"nummod","subj":"T20551","obj":"T20552"},{"id":"R6177","pred":"pobj","subj":"T20552","obj":"T20550"},{"id":"R6178","pred":"prep","subj":"T20553","obj":"T20547"},{"id":"R6179","pred":"nummod","subj":"T20554","obj":"T20555"},{"id":"R6180","pred":"pobj","subj":"T20555","obj":"T20553"},{"id":"R6181","pred":"prep","subj":"T20556","obj":"T20547"},{"id":"R6182","pred":"nummod","subj":"T20557","obj":"T20558"},{"id":"R6183","pred":"pobj","subj":"T20558","obj":"T20556"},{"id":"R6184","pred":"prep","subj":"T20559","obj":"T20558"},{"id":"R6185","pred":"compound","subj":"T20560","obj":"T20561"},{"id":"R6186","pred":"pobj","subj":"T20561","obj":"T20559"},{"id":"R6187","pred":"punct","subj":"T20562","obj":"T20563"},{"id":"R6188","pred":"parataxis","subj":"T20563","obj":"T20561"},{"id":"R6189","pred":"nummod","subj":"T20564","obj":"T20565"},{"id":"R6190","pred":"compound","subj":"T20565","obj":"T20566"},{"id":"R6191","pred":"dep","subj":"T20566","obj":"T20563"},{"id":"R6192","pred":"compound","subj":"T20567","obj":"T20566"},{"id":"R6193","pred":"punct","subj":"T20568","obj":"T20566"},{"id":"R6194","pred":"punct","subj":"T20569","obj":"T20563"},{"id":"R6195","pred":"nummod","subj":"T20570","obj":"T20571"},{"id":"R6196","pred":"compound","subj":"T20571","obj":"T20572"},{"id":"R6197","pred":"dep","subj":"T20572","obj":"T20563"},{"id":"R6198","pred":"compound","subj":"T20573","obj":"T20572"},{"id":"R6199","pred":"punct","subj":"T20574","obj":"T20572"},{"id":"R6200","pred":"nummod","subj":"T20575","obj":"T20572"},{"id":"R6201","pred":"punct","subj":"T20576","obj":"T20563"},{"id":"R6202","pred":"nummod","subj":"T20577","obj":"T20578"},{"id":"R6203","pred":"nmod","subj":"T20578","obj":"T20579"},{"id":"R6204","pred":"dep","subj":"T20579","obj":"T20563"},{"id":"R6205","pred":"punct","subj":"T20580","obj":"T20581"},{"id":"R6206","pred":"prep","subj":"T20581","obj":"T20578"},{"id":"R6207","pred":"compound","subj":"T20582","obj":"T20579"},{"id":"R6208","pred":"punct","subj":"T20583","obj":"T20563"},{"id":"R6209","pred":"nummod","subj":"T20584","obj":"T20563"},{"id":"R6210","pred":"punct","subj":"T20585","obj":"T20563"},{"id":"R6211","pred":"punct","subj":"T20586","obj":"T20561"},{"id":"R6212","pred":"acl","subj":"T20587","obj":"T20561"},{"id":"R6213","pred":"agent","subj":"T20588","obj":"T20587"},{"id":"R6214","pred":"nummod","subj":"T20589","obj":"T20590"},{"id":"R6215","pred":"pobj","subj":"T20590","obj":"T20588"},{"id":"R6216","pred":"prep","subj":"T20591","obj":"T20590"},{"id":"R6217","pred":"nummod","subj":"T20592","obj":"T20593"},{"id":"R6218","pred":"pobj","subj":"T20593","obj":"T20591"},{"id":"R6219","pred":"aux","subj":"T20594","obj":"T20595"},{"id":"R6220","pred":"advcl","subj":"T20595","obj":"T20587"},{"id":"R6221","pred":"det","subj":"T20596","obj":"T20597"},{"id":"R6222","pred":"dobj","subj":"T20597","obj":"T20595"},{"id":"R6223","pred":"compound","subj":"T20598","obj":"T20597"},{"id":"R6224","pred":"punct","subj":"T20599","obj":"T20547"}],"attributes":[{"subj":"T20576","pred":"source","obj":"craft-sa-dev"},{"subj":"T20575","pred":"source","obj":"craft-sa-dev"},{"subj":"T20574","pred":"source","obj":"craft-sa-dev"},{"subj":"T20573","pred":"source","obj":"craft-sa-dev"},{"subj":"T20572","pred":"source","obj":"craft-sa-dev"},{"subj":"T20571","pred":"source","obj":"craft-sa-dev"},{"subj":"T20570","pred":"source","obj":"craft-sa-dev"},{"subj":"T20569","pred":"source","obj":"craft-sa-dev"},{"subj":"T20568","pred":"source","obj":"craft-sa-dev"},{"subj":"T20567","pred":"source","obj":"craft-sa-dev"},{"subj":"T20566","pred":"source","obj":"craft-sa-dev"},{"subj":"T20565","pred":"source","obj":"craft-sa-dev"},{"subj":"T20564","pred":"source","obj":"craft-sa-dev"},{"subj":"T20563","pred":"source","obj":"craft-sa-dev"},{"subj":"T20562","pred":"source","obj":"craft-sa-dev"},{"subj":"T20561","pred":"source","obj":"craft-sa-dev"},{"subj":"T20560","pred":"source","obj":"craft-sa-dev"},{"subj":"T20559","pred":"source","obj":"craft-sa-dev"},{"subj":"T20558","pred":"source","obj":"craft-sa-dev"},{"subj":"T20557","pred":"source","obj":"craft-sa-dev"},{"subj":"T20556","pred":"source","obj":"craft-sa-dev"},{"subj":"T20555","pred":"source","obj":"craft-sa-dev"},{"subj":"T20554","pred":"source","obj":"craft-sa-dev"},{"subj":"T20553","pred":"source","obj":"craft-sa-dev"},{"subj":"T20552","pred":"source","obj":"craft-sa-dev"},{"subj":"T20551","pred":"source","obj":"craft-sa-dev"},{"subj":"T20550","pred":"source","obj":"craft-sa-dev"},{"subj":"T20549","pred":"source","obj":"craft-sa-dev"},{"subj":"T20548","pred":"source","obj":"craft-sa-dev"},{"subj":"T20547","pred":"source","obj":"craft-sa-dev"},{"subj":"T20546","pred":"source","obj":"craft-sa-dev"},{"subj":"T20545","pred":"source","obj":"craft-sa-dev"},{"subj":"T20544","pred":"source","obj":"craft-sa-dev"},{"subj":"T20599","pred":"source","obj":"craft-sa-dev"},{"subj":"T20598","pred":"source","obj":"craft-sa-dev"},{"subj":"T20597","pred":"source","obj":"craft-sa-dev"},{"subj":"T20596","pred":"source","obj":"craft-sa-dev"},{"subj":"T20595","pred":"source","obj":"craft-sa-dev"},{"subj":"T20594","pred":"source","obj":"craft-sa-dev"},{"subj":"T20593","pred":"source","obj":"craft-sa-dev"},{"subj":"T20592","pred":"source","obj":"craft-sa-dev"},{"subj":"T20591","pred":"source","obj":"craft-sa-dev"},{"subj":"T20590","pred":"source","obj":"craft-sa-dev"},{"subj":"T20589","pred":"source","obj":"craft-sa-dev"},{"subj":"T20588","pred":"source","obj":"craft-sa-dev"},{"subj":"T20587","pred":"source","obj":"craft-sa-dev"},{"subj":"T20586","pred":"source","obj":"craft-sa-dev"},{"subj":"T20585","pred":"source","obj":"craft-sa-dev"},{"subj":"T20584","pred":"source","obj":"craft-sa-dev"},{"subj":"T20583","pred":"source","obj":"craft-sa-dev"},{"subj":"T20582","pred":"source","obj":"craft-sa-dev"},{"subj":"T20581","pred":"source","obj":"craft-sa-dev"},{"subj":"T20580","pred":"source","obj":"craft-sa-dev"},{"subj":"T20579","pred":"source","obj":"craft-sa-dev"},{"subj":"T20578","pred":"source","obj":"craft-sa-dev"},{"subj":"T20577","pred":"source","obj":"craft-sa-dev"}]},{"project":"craft-ca-core-dev","denotations":[{"id":"T20470","span":{"begin":107,"end":119},"obj":"CHEBI:9750"},{"id":"T20469","span":{"begin":97,"end":100},"obj":"CHEBI:17883"},{"id":"T20468","span":{"begin":92,"end":96},"obj":"CHEBI:9754"},{"id":"T20467","span":{"begin":72,"end":77},"obj":"GO:0019835"},{"id":"T20466","span":{"begin":10,"end":17},"obj":"UBERON:0000922"}],"attributes":[{"subj":"T20470","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T20469","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T20468","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T20467","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T20466","pred":"source","obj":"craft-ca-core-dev"}]},{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T20491","span":{"begin":204,"end":214},"obj":"GO_EXT:0008233"},{"id":"T20490","span":{"begin":131,"end":141},"obj":"GO_EXT:0008233"},{"id":"T20489","span":{"begin":107,"end":119},"obj":"CHEBI:9750"},{"id":"T20488","span":{"begin":97,"end":100},"obj":"CHEBI:17883"},{"id":"T20487","span":{"begin":92,"end":96},"obj":"CHEBI:9754"},{"id":"T20486","span":{"begin":78,"end":84},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T20485","span":{"begin":72,"end":77},"obj":"GO:0019835"},{"id":"T20484","span":{"begin":10,"end":17},"obj":"UBERON:0000922"}],"attributes":[{"subj":"T20491","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T20490","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T20489","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T20488","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T20487","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T20486","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T20485","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T20484","pred":"source","obj":"craft-ca-core-ex-dev"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"craft-sa-dev","color":"#93d0ec","default":true},{"id":"craft-ca-core-dev","color":"#eaec93"},{"id":"craft-ca-core-ex-dev","color":"#d493ec"}]}]}}