PMC:1382200 / 39398-39615
Annnotations
craft-sa-dev
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20576 | 119-121 | , | denotes | , |
T20575 | 116-119 | CD | denotes | 100 |
T20574 | 115-116 | HYPH | denotes | - |
T20573 | 107-113 | NNP | denotes | Triton |
T20572 | 114-115 | NN | denotes | X |
T20571 | 105-106 | NN | denotes | % |
T20570 | 102-105 | CD | denotes | 0.5 |
T20569 | 100-102 | , | denotes | , |
T20568 | 96-97 | HYPH | denotes | - |
T20567 | 92-96 | NN | denotes | Tris |
T20566 | 97-100 | NN | denotes | HCl |
T20565 | 89-91 | NN | denotes | mM |
T20564 | 86-88 | CD | denotes | 50 |
T20563 | 145-147 | NN | denotes | pH |
T20562 | 85-86 | -LRB- | denotes | ( |
T20561 | 78-84 | NN | denotes | buffer |
T20560 | 72-77 | NN | denotes | lysis |
T20559 | 69-71 | IN | denotes | of |
T20558 | 66-68 | NN | denotes | μL |
T20557 | 63-65 | CD | denotes | 20 |
T20556 | 60-62 | IN | denotes | in |
T20555 | 57-59 | NN | denotes | °C |
T20554 | 55-57 | CD | denotes | 55 |
T20553 | 52-54 | IN | denotes | at |
T20552 | 46-51 | NNS | denotes | hours |
T20551 | 41-45 | CD | denotes | five |
T20550 | 37-40 | IN | denotes | for |
T20549 | 23-27 | RB | denotes | then |
T20548 | 18-22 | VBD | denotes | were |
T20547 | 28-36 | VBN | denotes | digested |
T20546 | 10-17 | NNS | denotes | embryos |
T20545 | 1-9 | VBN | denotes | Isolated |
T20544 | 0-217 | sentence | denotes | Isolated embryos were then digested for five hours at 55°C in 20 μL of lysis buffer (50 mM Tris-HCl, 0.5% Triton X-100, 200 μL/mL proteinase K, pH 8.0), followed by ten minutes at 95°C to inactivate the proteinase K. |
T20599 | 216-217 | . | denotes | . |
T20598 | 204-214 | NN | denotes | proteinase |
T20597 | 215-216 | NN | denotes | K |
T20596 | 200-203 | DT | denotes | the |
T20595 | 189-199 | VB | denotes | inactivate |
T20594 | 186-188 | TO | denotes | to |
T20593 | 183-185 | NN | denotes | °C |
T20592 | 181-183 | CD | denotes | 95 |
T20591 | 178-180 | IN | denotes | at |
T20590 | 170-177 | NNS | denotes | minutes |
T20589 | 166-169 | CD | denotes | ten |
T20588 | 163-165 | IN | denotes | by |
T20587 | 154-162 | VBN | denotes | followed |
T20586 | 152-154 | , | denotes | , |
T20585 | 151-152 | -RRB- | denotes | ) |
T20584 | 148-151 | CD | denotes | 8.0 |
T20583 | 143-145 | , | denotes | , |
T20582 | 131-141 | NN | denotes | proteinase |
T20581 | 128-130 | NN | denotes | mL |
T20580 | 127-128 | SYM | denotes | / |
T20579 | 142-143 | NN | denotes | K |
T20578 | 125-127 | NN | denotes | μL |
T20577 | 121-124 | CD | denotes | 200 |
R6171 | T20545 | T20546 | amod | Isolated,embryos |
R6172 | T20546 | T20547 | nsubjpass | embryos,digested |
R6173 | T20548 | T20547 | auxpass | were,digested |
R6174 | T20549 | T20547 | advmod | then,digested |
R6175 | T20550 | T20547 | prep | for,digested |
R6176 | T20551 | T20552 | nummod | five,hours |
R6177 | T20552 | T20550 | pobj | hours,for |
R6178 | T20553 | T20547 | prep | at,digested |
R6179 | T20554 | T20555 | nummod | 55,°C |
R6180 | T20555 | T20553 | pobj | °C,at |
R6181 | T20556 | T20547 | prep | in,digested |
R6182 | T20557 | T20558 | nummod | 20,μL |
R6183 | T20558 | T20556 | pobj | μL,in |
R6184 | T20559 | T20558 | prep | of,μL |
R6185 | T20560 | T20561 | compound | lysis,buffer |
R6186 | T20561 | T20559 | pobj | buffer,of |
R6187 | T20562 | T20563 | punct | (,pH |
R6188 | T20563 | T20561 | parataxis | pH,buffer |
R6189 | T20564 | T20565 | nummod | 50,mM |
R6190 | T20565 | T20566 | compound | mM,HCl |
R6191 | T20566 | T20563 | dep | HCl,pH |
R6192 | T20567 | T20566 | compound | Tris,HCl |
R6193 | T20568 | T20566 | punct | -,HCl |
R6194 | T20569 | T20563 | punct | ", ",pH |
R6195 | T20570 | T20571 | nummod | 0.5,% |
R6196 | T20571 | T20572 | compound | %,X |
R6197 | T20572 | T20563 | dep | X,pH |
R6198 | T20573 | T20572 | compound | Triton,X |
R6199 | T20574 | T20572 | punct | -,X |
R6200 | T20575 | T20572 | nummod | 100,X |
R6201 | T20576 | T20563 | punct | ", ",pH |
R6202 | T20577 | T20578 | nummod | 200,μL |
R6203 | T20578 | T20579 | nmod | μL,K |
R6204 | T20579 | T20563 | dep | K,pH |
R6205 | T20580 | T20581 | punct | /,mL |
R6206 | T20581 | T20578 | prep | mL,μL |
R6207 | T20582 | T20579 | compound | proteinase,K |
R6208 | T20583 | T20563 | punct | ", ",pH |
R6209 | T20584 | T20563 | nummod | 8.0,pH |
R6210 | T20585 | T20563 | punct | ),pH |
R6211 | T20586 | T20561 | punct | ", ",buffer |
R6212 | T20587 | T20561 | acl | followed,buffer |
R6213 | T20588 | T20587 | agent | by,followed |
R6214 | T20589 | T20590 | nummod | ten,minutes |
R6215 | T20590 | T20588 | pobj | minutes,by |
R6216 | T20591 | T20590 | prep | at,minutes |
R6217 | T20592 | T20593 | nummod | 95,°C |
R6218 | T20593 | T20591 | pobj | °C,at |
R6219 | T20594 | T20595 | aux | to,inactivate |
R6220 | T20595 | T20587 | advcl | inactivate,followed |
R6221 | T20596 | T20597 | det | the,K |
R6222 | T20597 | T20595 | dobj | K,inactivate |
R6223 | T20598 | T20597 | compound | proteinase,K |
R6224 | T20599 | T20547 | punct | .,digested |
craft-ca-core-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20470 | 107-119 | CHEBI:9750 | denotes | Triton X-100 |
T20469 | 97-100 | CHEBI:17883 | denotes | HCl |
T20468 | 92-96 | CHEBI:9754 | denotes | Tris |
T20467 | 72-77 | GO:0019835 | denotes | lysis |
T20466 | 10-17 | UBERON:0000922 | denotes | embryos |
craft-ca-core-ex-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20491 | 204-214 | GO_EXT:0008233 | denotes | proteinase |
T20490 | 131-141 | GO_EXT:0008233 | denotes | proteinase |
T20489 | 107-119 | CHEBI:9750 | denotes | Triton X-100 |
T20488 | 97-100 | CHEBI:17883 | denotes | HCl |
T20487 | 92-96 | CHEBI:9754 | denotes | Tris |
T20486 | 78-84 | CHEBI_CHMO_EXT:buffer_solution | denotes | buffer |
T20485 | 72-77 | GO:0019835 | denotes | lysis |
T20484 | 10-17 | UBERON:0000922 | denotes | embryos |