PMC:1382200 / 38823-39094
Annnotations
craft-sa-dev
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20232 | 270-271 | . | denotes | . |
T20231 | 263-270 | NNS | denotes | minutes |
T20230 | 259-262 | CD | denotes | ten |
T20229 | 256-258 | IN | denotes | of |
T20228 | 241-250 | NN | denotes | extension |
T20227 | 235-240 | JJ | denotes | final |
T20226 | 251-255 | NN | denotes | step |
T20225 | 233-234 | DT | denotes | a |
T20224 | 228-232 | IN | denotes | with |
T20223 | 226-228 | , | denotes | , |
T20222 | 224-226 | NN | denotes | °C |
T20221 | 222-224 | CD | denotes | 72 |
T20220 | 219-221 | IN | denotes | at |
T20219 | 209-218 | NN | denotes | extension |
T20218 | 202-208 | NN | denotes | minute |
T20217 | 198-201 | CD | denotes | one |
T20216 | 194-197 | CC | denotes | and |
T20215 | 192-194 | , | denotes | , |
T20214 | 190-192 | NN | denotes | °C |
T20213 | 188-190 | CD | denotes | 56 |
T20212 | 185-187 | IN | denotes | at |
T20211 | 175-184 | VBG | denotes | annealing |
T20210 | 168-174 | NN | denotes | minute |
T20209 | 164-167 | CD | denotes | one |
T20208 | 162-164 | , | denotes | , |
T20207 | 160-162 | NN | denotes | °C |
T20206 | 158-160 | CD | denotes | 95 |
T20205 | 155-157 | IN | denotes | at |
T20204 | 135-141 | NN | denotes | minute |
T20203 | 142-154 | NN | denotes | denaturation |
T20202 | 131-134 | CD | denotes | one |
T20201 | 128-130 | IN | denotes | of |
T20200 | 121-127 | NNS | denotes | cycles |
T20199 | 118-120 | CD | denotes | 35 |
T20198 | 116-118 | , | denotes | , |
T20197 | 114-116 | NN | denotes | °C |
T20196 | 112-114 | CD | denotes | 95 |
T20195 | 109-111 | IN | denotes | at |
T20194 | 89-95 | NN | denotes | minute |
T20193 | 84-88 | CD | denotes | five |
T20192 | 76-83 | JJ | denotes | initial |
T20191 | 96-108 | NN | denotes | denaturation |
T20190 | 73-75 | DT | denotes | an |
T20189 | 63-72 | VBG | denotes | entailing |
T20188 | 48-51 | NN | denotes | PCR |
T20187 | 52-62 | NNS | denotes | conditions |
T20186 | 38-47 | JJ | denotes | identical |
T20185 | 32-37 | VBG | denotes | using |
T20184 | 28-31 | RP | denotes | out |
T20183 | 15-19 | VBD | denotes | were |
T20182 | 20-27 | VBN | denotes | carried |
T20181 | 5-14 | NNS | denotes | reactions |
T20180 | 1-4 | DT | denotes | All |
T20179 | 0-271 | sentence | denotes | All reactions were carried out using identical PCR conditions entailing an initial five minute denaturation at 95°C, 35 cycles of one minute denaturation at 95°C, one minute annealing at 56°C, and one minute extension at 72°C, with a final extension step of ten minutes. |
R6072 | T20180 | T20181 | det | All,reactions |
R6073 | T20181 | T20182 | nsubjpass | reactions,carried |
R6074 | T20183 | T20182 | auxpass | were,carried |
R6075 | T20184 | T20182 | prt | out,carried |
R6076 | T20185 | T20182 | advcl | using,carried |
R6077 | T20186 | T20187 | amod | identical,conditions |
R6078 | T20187 | T20185 | dobj | conditions,using |
R6079 | T20188 | T20187 | compound | PCR,conditions |
R6080 | T20189 | T20187 | acl | entailing,conditions |
R6081 | T20190 | T20191 | det | an,denaturation |
R6082 | T20191 | T20189 | dobj | denaturation,entailing |
R6083 | T20192 | T20191 | amod | initial,denaturation |
R6084 | T20193 | T20194 | nummod | five,minute |
R6085 | T20194 | T20191 | compound | minute,denaturation |
R6086 | T20195 | T20191 | prep | at,denaturation |
R6087 | T20196 | T20197 | nummod | 95,°C |
R6088 | T20197 | T20195 | pobj | °C,at |
R6089 | T20198 | T20191 | punct | ", ",denaturation |
R6090 | T20199 | T20200 | nummod | 35,cycles |
R6091 | T20200 | T20191 | conj | cycles,denaturation |
R6092 | T20201 | T20200 | prep | of,cycles |
R6093 | T20202 | T20203 | nummod | one,denaturation |
R6094 | T20203 | T20201 | pobj | denaturation,of |
R6095 | T20204 | T20203 | compound | minute,denaturation |
R6096 | T20205 | T20200 | prep | at,cycles |
R6097 | T20206 | T20207 | nummod | 95,°C |
R6098 | T20207 | T20205 | pobj | °C,at |
R6099 | T20208 | T20200 | punct | ", ",cycles |
R6100 | T20209 | T20210 | nummod | one,minute |
R6101 | T20210 | T20200 | conj | minute,cycles |
R6102 | T20211 | T20210 | amod | annealing,minute |
R6103 | T20212 | T20210 | prep | at,minute |
R6104 | T20213 | T20214 | nummod | 56,°C |
R6105 | T20214 | T20212 | pobj | °C,at |
R6106 | T20215 | T20210 | punct | ", ",minute |
R6107 | T20216 | T20210 | cc | and,minute |
R6108 | T20217 | T20218 | nummod | one,minute |
R6109 | T20218 | T20219 | compound | minute,extension |
R6110 | T20219 | T20210 | conj | extension,minute |
R6111 | T20220 | T20219 | prep | at,extension |
R6112 | T20221 | T20222 | nummod | 72,°C |
R6113 | T20222 | T20220 | pobj | °C,at |
R6114 | T20223 | T20189 | punct | ", ",entailing |
R6115 | T20224 | T20189 | prep | with,entailing |
R6116 | T20225 | T20226 | det | a,step |
R6117 | T20226 | T20224 | pobj | step,with |
R6118 | T20227 | T20226 | amod | final,step |
R6119 | T20228 | T20226 | compound | extension,step |
R6120 | T20229 | T20226 | prep | of,step |
R6121 | T20230 | T20231 | nummod | ten,minutes |
R6122 | T20231 | T20229 | pobj | minutes,of |
R6123 | T20232 | T20182 | punct | .,carried |
craft-ca-core-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19413 | 175-184 | GO:0097617 | denotes | annealing |
craft-ca-core-ex-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T19529 | 175-184 | GO:0097617 | denotes | annealing |
T19528 | 142-154 | GO_EXT:macromolecule_denaturation | denotes | denaturation |
T19527 | 96-108 | GO_EXT:macromolecule_denaturation | denotes | denaturation |