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PMC:1382200 / 38823-39094 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T20232 270-271 . denotes .
T20231 263-270 NNS denotes minutes
T20230 259-262 CD denotes ten
T20229 256-258 IN denotes of
T20228 241-250 NN denotes extension
T20227 235-240 JJ denotes final
T20226 251-255 NN denotes step
T20225 233-234 DT denotes a
T20224 228-232 IN denotes with
T20223 226-228 , denotes ,
T20222 224-226 NN denotes °C
T20221 222-224 CD denotes 72
T20220 219-221 IN denotes at
T20219 209-218 NN denotes extension
T20218 202-208 NN denotes minute
T20217 198-201 CD denotes one
T20216 194-197 CC denotes and
T20215 192-194 , denotes ,
T20214 190-192 NN denotes °C
T20213 188-190 CD denotes 56
T20212 185-187 IN denotes at
T20211 175-184 VBG denotes annealing
T20210 168-174 NN denotes minute
T20209 164-167 CD denotes one
T20208 162-164 , denotes ,
T20207 160-162 NN denotes °C
T20206 158-160 CD denotes 95
T20205 155-157 IN denotes at
T20204 135-141 NN denotes minute
T20203 142-154 NN denotes denaturation
T20202 131-134 CD denotes one
T20201 128-130 IN denotes of
T20200 121-127 NNS denotes cycles
T20199 118-120 CD denotes 35
T20198 116-118 , denotes ,
T20197 114-116 NN denotes °C
T20196 112-114 CD denotes 95
T20195 109-111 IN denotes at
T20194 89-95 NN denotes minute
T20193 84-88 CD denotes five
T20192 76-83 JJ denotes initial
T20191 96-108 NN denotes denaturation
T20190 73-75 DT denotes an
T20189 63-72 VBG denotes entailing
T20188 48-51 NN denotes PCR
T20187 52-62 NNS denotes conditions
T20186 38-47 JJ denotes identical
T20185 32-37 VBG denotes using
T20184 28-31 RP denotes out
T20183 15-19 VBD denotes were
T20182 20-27 VBN denotes carried
T20181 5-14 NNS denotes reactions
T20180 1-4 DT denotes All
T20179 0-271 sentence denotes All reactions were carried out using identical PCR conditions entailing an initial five minute denaturation at 95°C, 35 cycles of one minute denaturation at 95°C, one minute annealing at 56°C, and one minute extension at 72°C, with a final extension step of ten minutes.
R6072 T20180 T20181 det All,reactions
R6073 T20181 T20182 nsubjpass reactions,carried
R6074 T20183 T20182 auxpass were,carried
R6075 T20184 T20182 prt out,carried
R6076 T20185 T20182 advcl using,carried
R6077 T20186 T20187 amod identical,conditions
R6078 T20187 T20185 dobj conditions,using
R6079 T20188 T20187 compound PCR,conditions
R6080 T20189 T20187 acl entailing,conditions
R6081 T20190 T20191 det an,denaturation
R6082 T20191 T20189 dobj denaturation,entailing
R6083 T20192 T20191 amod initial,denaturation
R6084 T20193 T20194 nummod five,minute
R6085 T20194 T20191 compound minute,denaturation
R6086 T20195 T20191 prep at,denaturation
R6087 T20196 T20197 nummod 95,°C
R6088 T20197 T20195 pobj °C,at
R6089 T20198 T20191 punct ", ",denaturation
R6090 T20199 T20200 nummod 35,cycles
R6091 T20200 T20191 conj cycles,denaturation
R6092 T20201 T20200 prep of,cycles
R6093 T20202 T20203 nummod one,denaturation
R6094 T20203 T20201 pobj denaturation,of
R6095 T20204 T20203 compound minute,denaturation
R6096 T20205 T20200 prep at,cycles
R6097 T20206 T20207 nummod 95,°C
R6098 T20207 T20205 pobj °C,at
R6099 T20208 T20200 punct ", ",cycles
R6100 T20209 T20210 nummod one,minute
R6101 T20210 T20200 conj minute,cycles
R6102 T20211 T20210 amod annealing,minute
R6103 T20212 T20210 prep at,minute
R6104 T20213 T20214 nummod 56,°C
R6105 T20214 T20212 pobj °C,at
R6106 T20215 T20210 punct ", ",minute
R6107 T20216 T20210 cc and,minute
R6108 T20217 T20218 nummod one,minute
R6109 T20218 T20219 compound minute,extension
R6110 T20219 T20210 conj extension,minute
R6111 T20220 T20219 prep at,extension
R6112 T20221 T20222 nummod 72,°C
R6113 T20222 T20220 pobj °C,at
R6114 T20223 T20189 punct ", ",entailing
R6115 T20224 T20189 prep with,entailing
R6116 T20225 T20226 det a,step
R6117 T20226 T20224 pobj step,with
R6118 T20227 T20226 amod final,step
R6119 T20228 T20226 compound extension,step
R6120 T20229 T20226 prep of,step
R6121 T20230 T20231 nummod ten,minutes
R6122 T20231 T20229 pobj minutes,of
R6123 T20232 T20182 punct .,carried

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T19413 175-184 GO:0097617 denotes annealing

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T19529 175-184 GO:0097617 denotes annealing
T19528 142-154 GO_EXT:macromolecule_denaturation denotes denaturation
T19527 96-108 GO_EXT:macromolecule_denaturation denotes denaturation