PMC:1382200 / 36053-37043 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T23680","span":{"begin":54,"end":59},"obj":"NN"},{"id":"T23679","span":{"begin":60,"end":65},"obj":"NN"},{"id":"T23678","span":{"begin":50,"end":53},"obj":"DT"},{"id":"T23677","span":{"begin":41,"end":49},"obj":"VB"},{"id":"T23676","span":{"begin":38,"end":40},"obj":"TO"},{"id":"T23675","span":{"begin":33,"end":37},"obj":"VBN"},{"id":"T23674","span":{"begin":25,"end":32},"obj":"NNS"},{"id":"T23673","span":{"begin":9,"end":24},"obj":"NN"}],"relations":[{"id":"R7153","pred":"compound","subj":"T23673","obj":"T23674"},{"id":"R7154","pred":"acl","subj":"T23675","obj":"T23674"},{"id":"R7155","pred":"aux","subj":"T23676","obj":"T23677"},{"id":"R7156","pred":"advcl","subj":"T23677","obj":"T23675"},{"id":"R7157","pred":"det","subj":"T23678","obj":"T23679"},{"id":"R7158","pred":"dobj","subj":"T23679","obj":"T23677"},{"id":"R7159","pred":"compound","subj":"T23680","obj":"T23679"}],"text":"Table 1 Oligonucleotide primers used to genotype the Capn2 locus\nAllele Primer Location Oligonucleotide Sequence\nSingle-Step\nBoth Sense Intron 4 5'-GGGCCCCCATTGCCTCTTAGC-3'\nWild-type Antisense Exon 7 5'- GGATTCCTGATGCGGATCAATTTCTGC-3'\nMutant Antisense PGK-Neo 5'-CCTCGAAGTCGAGGTCGATCC-3'\nNested PCR\nWild-Type (Diagnostic) Sense #1 Intron 6 5'-CAACATCATAAGCAACGGAGAACGC-3'\nSense #2 Intron 6 5'-GCCTGTGACAGAAGTACCACCAG-3'\nAntisense #1 Intron 7 5'-CTCCTCGGCCCTCCCTGTAG-3'\nAntisense #2 Exon 7 5'-GGATTCCTGATGCGGATCAATTTCTGC-3'\nMutant (Diagnostic) Sense #1 Intron 6 5'-CAACATCATAAGCAACGGAGAACGC-3'\nSense #2 Intron 6 5'-GCCTGTGACAGAAGTACCACCAG-3'\nAntisense #1 PGK-Neo 5'-CCTACCCGGTAGAATTGACCTGC-3'\nAntisense #2 PGK-Neo 5'-GACCTGCAGGGGCCCTC-3'\nBoth (Internal Control) Sense #1 Intron 4 5-GGGATGAAGGCTCCCTGTTGC-3'\nSense #2 Exon 5 5'-GGGGGTGCCACCACGGAA -3'\nAntisense #1 Intron 5 5'-CCCCTAGAGGACTATTGCCTGG-3'\nAntisense #2 Intron 5 5'-GATGCACCCATGCCTTAAGGAGC-3'"}

{"project":"craft-ca-core-dev","denotations":[{"id":"T23669","span":{"begin":54,"end":59},"obj":"PR:000005015"},{"id":"T23668","span":{"begin":25,"end":32},"obj":"SO:0000112"}],"text":"Table 1 Oligonucleotide primers used to genotype the Capn2 locus\nAllele Primer Location Oligonucleotide Sequence\nSingle-Step\nBoth Sense Intron 4 5'-GGGCCCCCATTGCCTCTTAGC-3'\nWild-type Antisense Exon 7 5'- GGATTCCTGATGCGGATCAATTTCTGC-3'\nMutant Antisense PGK-Neo 5'-CCTCGAAGTCGAGGTCGATCC-3'\nNested PCR\nWild-Type (Diagnostic) Sense #1 Intron 6 5'-CAACATCATAAGCAACGGAGAACGC-3'\nSense #2 Intron 6 5'-GCCTGTGACAGAAGTACCACCAG-3'\nAntisense #1 Intron 7 5'-CTCCTCGGCCCTCCCTGTAG-3'\nAntisense #2 Exon 7 5'-GGATTCCTGATGCGGATCAATTTCTGC-3'\nMutant (Diagnostic) Sense #1 Intron 6 5'-CAACATCATAAGCAACGGAGAACGC-3'\nSense #2 Intron 6 5'-GCCTGTGACAGAAGTACCACCAG-3'\nAntisense #1 PGK-Neo 5'-CCTACCCGGTAGAATTGACCTGC-3'\nAntisense #2 PGK-Neo 5'-GACCTGCAGGGGCCCTC-3'\nBoth (Internal Control) Sense #1 Intron 4 5-GGGATGAAGGCTCCCTGTTGC-3'\nSense #2 Exon 5 5'-GGGGGTGCCACCACGGAA -3'\nAntisense #1 Intron 5 5'-CCCCTAGAGGACTATTGCCTGG-3'\nAntisense #2 Intron 5 5'-GATGCACCCATGCCTTAAGGAGC-3'"}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T23671","span":{"begin":25,"end":32},"obj":"SO_EXT:0000112"},{"id":"T23670","span":{"begin":9,"end":24},"obj":"CHEBI_SO_EXT:oligonucleotide"},{"id":"T23672","span":{"begin":54,"end":59},"obj":"PR_EXT:000005015"}],"text":"Table 1 Oligonucleotide primers used to genotype the Capn2 locus\nAllele Primer Location Oligonucleotide Sequence\nSingle-Step\nBoth Sense Intron 4 5'-GGGCCCCCATTGCCTCTTAGC-3'\nWild-type Antisense Exon 7 5'- GGATTCCTGATGCGGATCAATTTCTGC-3'\nMutant Antisense PGK-Neo 5'-CCTCGAAGTCGAGGTCGATCC-3'\nNested PCR\nWild-Type (Diagnostic) Sense #1 Intron 6 5'-CAACATCATAAGCAACGGAGAACGC-3'\nSense #2 Intron 6 5'-GCCTGTGACAGAAGTACCACCAG-3'\nAntisense #1 Intron 7 5'-CTCCTCGGCCCTCCCTGTAG-3'\nAntisense #2 Exon 7 5'-GGATTCCTGATGCGGATCAATTTCTGC-3'\nMutant (Diagnostic) Sense #1 Intron 6 5'-CAACATCATAAGCAACGGAGAACGC-3'\nSense #2 Intron 6 5'-GCCTGTGACAGAAGTACCACCAG-3'\nAntisense #1 PGK-Neo 5'-CCTACCCGGTAGAATTGACCTGC-3'\nAntisense #2 PGK-Neo 5'-GACCTGCAGGGGCCCTC-3'\nBoth (Internal Control) Sense #1 Intron 4 5-GGGATGAAGGCTCCCTGTTGC-3'\nSense #2 Exon 5 5'-GGGGGTGCCACCACGGAA -3'\nAntisense #1 Intron 5 5'-CCCCTAGAGGACTATTGCCTGG-3'\nAntisense #2 Intron 5 5'-GATGCACCCATGCCTTAAGGAGC-3'"}