Table 1  Oligonucleotide primers used to genotype the Capn2 locus
Allele  Primer  Location  Oligonucleotide Sequence
Single-Step
Both  Sense  Intron 4  5'-GGGCCCCCATTGCCTCTTAGC-3'
Wild-type  Antisense  Exon 7  5'- GGATTCCTGATGCGGATCAATTTCTGC-3'
Mutant  Antisense  PGK-Neo  5'-CCTCGAAGTCGAGGTCGATCC-3'
Nested PCR
Wild-Type (Diagnostic)  Sense #1  Intron 6  5'-CAACATCATAAGCAACGGAGAACGC-3'
Sense #2  Intron 6  5'-GCCTGTGACAGAAGTACCACCAG-3'
Antisense #1  Intron 7  5'-CTCCTCGGCCCTCCCTGTAG-3'
Antisense #2  Exon 7  5'-GGATTCCTGATGCGGATCAATTTCTGC-3'
Mutant (Diagnostic)  Sense #1  Intron 6  5'-CAACATCATAAGCAACGGAGAACGC-3'
Sense #2  Intron 6  5'-GCCTGTGACAGAAGTACCACCAG-3'
Antisense #1  PGK-Neo  5'-CCTACCCGGTAGAATTGACCTGC-3'
Antisense #2  PGK-Neo  5'-GACCTGCAGGGGCCCTC-3'
Both (Internal Control)  Sense #1  Intron 4  5-GGGATGAAGGCTCCCTGTTGC-3'
Sense #2  Exon 5  5'-GGGGGTGCCACCACGGAA -3'
Antisense #1  Intron 5  5'-CCCCTAGAGGACTATTGCCTGG-3'
Antisense #2  Intron 5  5'-GATGCACCCATGCCTTAAGGAGC-3'