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PMC:1382200 / 35225-36052 JSONTXT

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Id Subject Object Predicate Lexical cue
T19768 588-589 -RRB- denotes )
T19767 581-586 NN denotes Table
T19766 587-588 NN denotes I
T19765 580-581 -LRB- denotes (
T19764 569-570 -RRB- denotes )
T19763 565-566 HYPH denotes -
T19762 562-565 NN denotes PGK
T19761 566-569 NN denotes Neo
T19760 561-562 -LRB- denotes (
T19759 554-560 NN denotes mutant
T19758 551-553 CC denotes or
T19757 549-550 -RRB- denotes )
T19756 548-549 CD denotes 7
T19755 543-547 NN denotes exon
T19754 542-543 -LRB- denotes (
T19753 571-579 NN denotes sequence
T19752 536-537 HYPH denotes -
T19751 532-536 JJ denotes wild
T19750 537-541 NN denotes type
T19749 525-531 CC denotes either
T19748 522-524 IN denotes to
T19747 511-521 VBD denotes hybridized
T19746 505-510 WDT denotes which
T19745 487-496 NN denotes antisense
T19744 497-504 NNS denotes primers
T19743 478-486 JJ denotes distinct
T19742 474-477 CC denotes and
T19741 472-474 , denotes ,
T19740 464-472 NN denotes homology
T19739 461-463 IN denotes of
T19738 451-456 JJ denotes short
T19737 457-460 NN denotes arm
T19736 447-450 DT denotes the
T19735 439-446 IN denotes outside
T19734 431-438 VBN denotes located
T19733 429-431 , denotes ,
T19732 415-416 -RRB- denotes )
T19731 414-415 CD denotes 4
T19730 407-413 NN denotes intron
T19729 406-407 -LRB- denotes (
T19728 417-422 NN denotes sense
T19727 399-405 JJ denotes common
T19726 423-429 NN denotes primer
T19725 397-398 DT denotes a
T19724 391-396 VBG denotes using
T19723 381-390 NNS denotes reactions
T19722 372-380 JJ denotes separate
T19721 369-371 IN denotes in
T19720 354-358 VBD denotes were
T19719 340-346 NN denotes mutant
T19718 347-353 NN denotes allele
T19717 336-339 DT denotes the
T19716 333-335 IN denotes of
T19715 322-324 NN denotes bp
T19714 316-321 CD denotes 2,711
T19713 325-332 NN denotes segment
T19712 314-315 DT denotes a
T19711 310-313 CC denotes and
T19710 297-298 HYPH denotes -
T19709 298-302 NN denotes type
T19708 293-297 JJ denotes wild
T19707 303-309 NN denotes allele
T19706 289-292 DT denotes the
T19705 286-288 IN denotes of
T19704 359-368 VBN denotes amplified
T19703 275-277 NN denotes bp
T19702 269-274 CD denotes 2,748
T19701 278-285 NN denotes segment
T19700 267-268 DT denotes A
T19699 266-590 sentence denotes A 2,748 bp segment of the wild-type allele and a 2,711 bp segment of the mutant allele were amplified in separate reactions using a common (intron 4) sense primer, located outside the short arm of homology, and distinct antisense primers which hybridized to either wild-type (exon 7) or mutant (PGK-Neo) sequence (Table I).
T19698 265-266 . denotes .
T19697 264-265 -RRB- denotes )
T19696 263-264 CD denotes 1
T19695 256-262 NN denotes Figure
T19694 255-256 -LRB- denotes (
T19693 245-254 NNS denotes weanlings
T19692 241-244 CC denotes and
T19691 233-240 NNS denotes embryos
T19690 215-232 JJ denotes post-implantation
T19689 210-214 IN denotes from
T19688 201-209 NNS denotes biopsies
T19687 198-200 CC denotes or
T19686 192-197 NNS denotes cells
T19685 189-191 NN denotes ES
T19684 178-188 VBG denotes genotyping
T19683 174-177 IN denotes for
T19682 163-173 JJ denotes sufficient
T19681 159-162 VBD denotes was
T19680 146-149 NN denotes PCR
T19679 140-141 HYPH denotes -
T19678 141-145 NN denotes step
T19677 134-140 JJ denotes single
T19676 150-158 NN denotes strategy
T19675 132-133 DT denotes A
T19674 131-266 sentence denotes A single-step PCR strategy was sufficient for genotyping ES cells or biopsies from post-implantation embryos and weanlings (Figure 1).
T19673 130-131 . denotes .
T19672 129-130 CD denotes 1
T19671 123-128 NN denotes Table
T19670 120-122 IN denotes in
T19669 109-112 VBP denotes are
T19668 85-100 NN denotes oligonucleotide
T19667 101-108 NNS denotes primers
T19666 81-84 DT denotes all
T19665 78-80 IN denotes of
T19664 113-119 VBN denotes listed
T19663 68-77 NNS denotes sequences
T19662 64-67 DT denotes The
T19661 63-131 sentence denotes The sequences of all oligonucleotide primers are listed in Table 1.
T19660 62-63 . denotes .
T19659 59-62 NN denotes DNA
T19658 51-58 JJ denotes genomic
T19657 48-50 IN denotes of
T19656 39-47 NN denotes analysis
T19655 35-38 NN denotes PCR
T19654 32-34 IN denotes by
T19653 28-31 RP denotes out
T19652 15-19 RB denotes also
T19651 11-14 VBD denotes was
T19650 20-27 VBN denotes carried
T19649 0-10 NN denotes Genotyping
T19817 826-827 . denotes .
T19816 812-821 NN denotes extension
T19815 806-811 JJ denotes final
T19814 799-805 NN denotes minute
T19813 795-798 CD denotes ten
T19812 822-826 NN denotes step
T19811 793-794 DT denotes a
T19810 788-792 IN denotes with
T19809 786-788 , denotes ,
T19808 784-786 NN denotes °C
T19807 782-784 CD denotes 72
T19806 779-781 IN denotes at
T19805 769-778 NN denotes extension
T19804 762-768 NN denotes minute
T19793 720-722 NN denotes °C
T19792 718-720 CD denotes 95
T19791 715-717 IN denotes at
T19790 702-714 NN denotes denaturation
T19789 695-701 NN denotes minute
T19788 691-694 CD denotes one
T19787 688-690 IN denotes of
T19786 681-687 NNS denotes cycles
T19785 678-680 CD denotes 30
T19784 676-678 , denotes ,
T19783 674-676 NN denotes °C
T19782 672-674 CD denotes 95
T19781 669-671 IN denotes at
T19780 651-663 NN denotes denaturation
T19779 643-650 JJ denotes initial
T19778 636-642 NN denotes minute
T19777 631-635 CD denotes five
T19776 664-668 NN denotes step
T19775 629-630 DT denotes a
T19774 620-628 VBD denotes included
T19773 595-608 VBG denotes thermocycling
T19772 609-619 NNS denotes parameters
T19771 591-594 DT denotes The
T19770 590-827 sentence denotes The thermocycling parameters included a five minute initial denaturation step at 95°C, 30 cycles of one minute denaturation at 95°C, one minute annealing at 56°C, and one minute extension at 72°C, with a ten minute final extension step.
T19769 589-590 . denotes .
T19803 758-761 CD denotes one
T19802 754-757 CC denotes and
T19801 752-754 , denotes ,
T19800 750-752 NN denotes °C
T19799 748-750 CD denotes 56
T19798 745-747 IN denotes at
T19797 735-744 VBG denotes annealing
T19796 728-734 NN denotes minute
T19795 724-727 CD denotes one
T19794 722-724 , denotes ,
R5578 T19649 T19650 nsubjpass Genotyping,carried
R5579 T19651 T19650 auxpass was,carried
R5580 T19652 T19650 advmod also,carried
R5581 T19653 T19650 prt out,carried
R5582 T19654 T19650 agent by,carried
R5583 T19655 T19656 compound PCR,analysis
R5584 T19656 T19654 pobj analysis,by
R5585 T19657 T19656 prep of,analysis
R5586 T19658 T19659 amod genomic,DNA
R5587 T19659 T19657 pobj DNA,of
R5588 T19660 T19650 punct .,carried
R5589 T19662 T19663 det The,sequences
R5590 T19663 T19664 nsubjpass sequences,listed
R5591 T19665 T19663 prep of,sequences
R5592 T19666 T19667 det all,primers
R5593 T19667 T19665 pobj primers,of
R5594 T19668 T19667 compound oligonucleotide,primers
R5595 T19669 T19664 auxpass are,listed
R5596 T19670 T19664 prep in,listed
R5597 T19671 T19670 pobj Table,in
R5598 T19672 T19671 nummod 1,Table
R5599 T19673 T19664 punct .,listed
R5600 T19675 T19676 det A,strategy
R5601 T19676 T19681 nsubj strategy,was
R5602 T19677 T19678 amod single,step
R5603 T19678 T19676 compound step,strategy
R5604 T19679 T19678 punct -,step
R5605 T19680 T19676 compound PCR,strategy
R5606 T19682 T19681 acomp sufficient,was
R5607 T19683 T19682 prep for,sufficient
R5608 T19684 T19683 pcomp genotyping,for
R5609 T19685 T19686 compound ES,cells
R5610 T19686 T19684 dobj cells,genotyping
R5611 T19687 T19686 cc or,cells
R5612 T19688 T19686 conj biopsies,cells
R5613 T19689 T19688 prep from,biopsies
R5614 T19690 T19691 amod post-implantation,embryos
R5615 T19691 T19689 pobj embryos,from
R5616 T19692 T19691 cc and,embryos
R5617 T19693 T19691 conj weanlings,embryos
R5618 T19694 T19695 punct (,Figure
R5619 T19695 T19684 parataxis Figure,genotyping
R5620 T19696 T19695 nummod 1,Figure
R5621 T19697 T19695 punct ),Figure
R5622 T19698 T19681 punct .,was
R5623 T19700 T19701 det A,segment
R5624 T19701 T19704 nsubjpass segment,amplified
R5625 T19702 T19703 nummod "2,748",bp
R5626 T19703 T19701 compound bp,segment
R5627 T19705 T19701 prep of,segment
R5628 T19706 T19707 det the,allele
R5629 T19707 T19705 pobj allele,of
R5630 T19708 T19709 amod wild,type
R5631 T19709 T19707 compound type,allele
R5632 T19710 T19709 punct -,type
R5633 T19711 T19701 cc and,segment
R5634 T19712 T19713 det a,segment
R5635 T19713 T19701 conj segment,segment
R5636 T19714 T19715 nummod "2,711",bp
R5637 T19715 T19713 compound bp,segment
R5638 T19716 T19713 prep of,segment
R5639 T19717 T19718 det the,allele
R5640 T19718 T19716 pobj allele,of
R5641 T19719 T19718 compound mutant,allele
R5642 T19720 T19704 auxpass were,amplified
R5643 T19721 T19704 prep in,amplified
R5644 T19722 T19723 amod separate,reactions
R5645 T19723 T19721 pobj reactions,in
R5646 T19724 T19704 advcl using,amplified
R5647 T19725 T19726 det a,primer
R5648 T19726 T19724 dobj primer,using
R5649 T19727 T19728 amod common,sense
R5650 T19728 T19726 compound sense,primer
R5651 T19729 T19730 punct (,intron
R5652 T19730 T19728 parataxis intron,sense
R5653 T19731 T19730 nummod 4,intron
R5654 T19732 T19730 punct ),intron
R5655 T19733 T19726 punct ", ",primer
R5656 T19734 T19726 acl located,primer
R5657 T19735 T19734 prep outside,located
R5658 T19736 T19737 det the,arm
R5659 T19737 T19735 pobj arm,outside
R5660 T19738 T19737 amod short,arm
R5661 T19739 T19737 prep of,arm
R5662 T19740 T19739 pobj homology,of
R5663 T19741 T19726 punct ", ",primer
R5664 T19742 T19726 cc and,primer
R5665 T19743 T19744 amod distinct,primers
R5666 T19744 T19726 conj primers,primer
R5667 T19745 T19744 compound antisense,primers
R5668 T19746 T19747 dep which,hybridized
R5669 T19747 T19744 relcl hybridized,primers
R5670 T19748 T19747 prep to,hybridized
R5671 T19749 T19750 preconj either,type
R5672 T19750 T19753 nmod type,sequence
R5673 T19751 T19750 amod wild,type
R5674 T19752 T19750 punct -,type
R5675 T19753 T19748 pobj sequence,to
R5676 T19754 T19755 punct (,exon
R5677 T19755 T19750 parataxis exon,type
R5678 T19756 T19755 nummod 7,exon
R5679 T19757 T19755 punct ),exon
R5680 T19758 T19750 cc or,type
R5681 T19759 T19750 conj mutant,type
R5682 T19760 T19761 punct (,Neo
R5683 T19761 T19759 parataxis Neo,mutant
R5684 T19762 T19761 compound PGK,Neo
R5685 T19763 T19761 punct -,Neo
R5686 T19764 T19761 punct ),Neo
R5687 T19765 T19766 punct (,I
R5688 T19766 T19747 parataxis I,hybridized
R5689 T19767 T19766 compound Table,I
R5690 T19768 T19766 punct ),I
R5691 T19769 T19704 punct .,amplified
R5692 T19771 T19772 det The,parameters
R5693 T19772 T19774 nsubj parameters,included
R5694 T19773 T19772 amod thermocycling,parameters
R5695 T19775 T19776 det a,step
R5696 T19776 T19774 dobj step,included
R5697 T19777 T19778 nummod five,minute
R5698 T19778 T19776 nmod minute,step
R5699 T19779 T19776 amod initial,step
R5700 T19780 T19776 compound denaturation,step
R5701 T19781 T19776 prep at,step
R5702 T19782 T19783 nummod 95,°C
R5703 T19783 T19781 pobj °C,at
R5704 T19784 T19776 punct ", ",step
R5705 T19785 T19786 nummod 30,cycles
R5706 T19786 T19776 conj cycles,step
R5707 T19787 T19786 prep of,cycles
R5716 T19796 T19786 conj minute,cycles
R5717 T19797 T19796 amod annealing,minute
R5718 T19798 T19796 prep at,minute
R5719 T19799 T19800 nummod 56,°C
R5720 T19800 T19798 pobj °C,at
R5721 T19801 T19796 punct ", ",minute
R5722 T19802 T19796 cc and,minute
R5723 T19803 T19804 nummod one,minute
R5724 T19804 T19805 compound minute,extension
R5725 T19805 T19796 conj extension,minute
R5726 T19806 T19805 prep at,extension
R5727 T19807 T19808 nummod 72,°C
R5728 T19808 T19806 pobj °C,at
R5729 T19809 T19776 punct ", ",step
R5730 T19810 T19776 prep with,step
R5731 T19811 T19812 det a,step
R5732 T19812 T19810 pobj step,with
R5733 T19813 T19814 nummod ten,minute
R5734 T19814 T19812 nmod minute,step
R5735 T19815 T19812 amod final,step
R5736 T19816 T19812 compound extension,step
R5737 T19817 T19774 punct .,included
R5708 T19788 T19789 nummod one,minute
R5709 T19789 T19790 compound minute,denaturation
R5710 T19790 T19787 pobj denaturation,of
R5711 T19791 T19786 prep at,cycles
R5712 T19792 T19793 nummod 95,°C
R5713 T19793 T19791 pobj °C,at
R5714 T19794 T19786 punct ", ",cycles
R5715 T19795 T19796 nummod one,minute

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T19358 407-413 SO:0000188 denotes intron
T19357 347-353 SO:0001023 denotes allele
T19356 322-324 SO:0000028 denotes bp
T19355 303-309 SO:0001023 denotes allele
T19354 275-277 SO:0000028 denotes bp
T19353 233-240 UBERON:0000922 denotes embryos
T19352 220-232 GO:0007566 denotes implantation
T19351 189-197 CL:0002322 denotes ES cells
T19350 101-108 SO:0000112 denotes primers
T19349 51-58 SO:0001026 denotes genomic
T19364 735-744 GO:0097617 denotes annealing
T19363 543-547 SO:0000147 denotes exon
T19362 511-521 GO:0097617 denotes hybridized
T19361 497-504 SO:0000112 denotes primers
T19360 487-496 SO:0000077 denotes antisense
T19359 423-429 SO:0000112 denotes primer

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T19462 735-744 GO:0097617 denotes annealing
T19461 702-714 GO_EXT:macromolecule_denaturation denotes denaturation
T19460 651-663 GO_EXT:macromolecule_denaturation denotes denaturation
T19459 571-579 SO_EXT:biological_sequence denotes sequence
T19458 566-569 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T19457 562-565 GO_EXT:phosphoglycerate_kinase denotes PGK
T19456 554-560 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T19455 543-547 SO_EXT:0000147 denotes exon
T19454 532-541 SO_EXT:wild_type_entity_or_quality denotes wild-type
T19453 511-521 GO:0097617 denotes hybridized
T19452 497-504 SO_EXT:0000112 denotes primers
T19451 487-496 SO:0000077 denotes antisense
T19450 423-429 SO_EXT:0000112 denotes primer
T19449 407-413 SO_EXT:0000188 denotes intron
T19448 347-353 SO_EXT:0001023 denotes allele
T19447 340-346 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T19446 322-324 SO_EXT:0000028 denotes bp
T19445 303-309 SO_EXT:0001023 denotes allele
T19444 293-302 SO_EXT:wild_type_entity_or_quality denotes wild-type
T19443 275-277 SO_EXT:0000028 denotes bp
T19442 233-240 UBERON:0000922 denotes embryos
T19441 220-232 GO:0007566 denotes implantation
T19440 192-197 CL_GO_EXT:cell denotes cells
T19439 189-197 CL:0002322 denotes ES cells
T19438 101-108 SO_EXT:0000112 denotes primers
T19437 85-100 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T19436 68-77 SO_EXT:biological_sequence denotes sequences
T19435 59-62 CHEBI_SO_EXT:DNA denotes DNA
T19434 51-62 SO_EXT:genomic_DNA denotes genomic DNA