PMC:1382200 / 35225-36052 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1382200","sourcedb":"PMC","sourceid":"1382200","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1382200","text":"Genotyping was also carried out by PCR analysis of genomic DNA. The sequences of all oligonucleotide primers are listed in Table 1. A single-step PCR strategy was sufficient for genotyping ES cells or biopsies from post-implantation embryos and weanlings (Figure 1). A 2,748 bp segment of the wild-type allele and a 2,711 bp segment of the mutant allele were amplified in separate reactions using a common (intron 4) sense primer, located outside the short arm of homology, and distinct antisense primers which hybridized to either wild-type (exon 7) or mutant (PGK-Neo) sequence (Table I). The thermocycling parameters included a five minute initial denaturation step at 95°C, 30 cycles of one minute denaturation at 95°C, one minute annealing at 56°C, and one minute extension at 72°C, with a ten minute final extension step.","tracks":[{"project":"craft-sa-dev","denotations":[{"id":"T19768","span":{"begin":588,"end":589},"obj":"-RRB-"},{"id":"T19767","span":{"begin":581,"end":586},"obj":"NN"},{"id":"T19766","span":{"begin":587,"end":588},"obj":"NN"},{"id":"T19765","span":{"begin":580,"end":581},"obj":"-LRB-"},{"id":"T19764","span":{"begin":569,"end":570},"obj":"-RRB-"},{"id":"T19763","span":{"begin":565,"end":566},"obj":"HYPH"},{"id":"T19762","span":{"begin":562,"end":565},"obj":"NN"},{"id":"T19761","span":{"begin":566,"end":569},"obj":"NN"},{"id":"T19760","span":{"begin":561,"end":562},"obj":"-LRB-"},{"id":"T19759","span":{"begin":554,"end":560},"obj":"NN"},{"id":"T19758","span":{"begin":551,"end":553},"obj":"CC"},{"id":"T19757","span":{"begin":549,"end":550},"obj":"-RRB-"},{"id":"T19756","span":{"begin":548,"end":549},"obj":"CD"},{"id":"T19755","span":{"begin":543,"end":547},"obj":"NN"},{"id":"T19754","span":{"begin":542,"end":543},"obj":"-LRB-"},{"id":"T19753","span":{"begin":571,"end":579},"obj":"NN"},{"id":"T19752","span":{"begin":536,"end":537},"obj":"HYPH"},{"id":"T19751","span":{"begin":532,"end":536},"obj":"JJ"},{"id":"T19750","span":{"begin":537,"end":541},"obj":"NN"},{"id":"T19749","span":{"begin":525,"end":531},"obj":"CC"},{"id":"T19748","span":{"begin":522,"end":524},"obj":"IN"},{"id":"T19747","span":{"begin":511,"end":521},"obj":"VBD"},{"id":"T19746","span":{"begin":505,"end":510},"obj":"WDT"},{"id":"T19745","span":{"begin":487,"end":496},"obj":"NN"},{"id":"T19744","span":{"begin":497,"end":504},"obj":"NNS"},{"id":"T19743","span":{"begin":478,"end":486},"obj":"JJ"},{"id":"T19742","span":{"begin":474,"end":477},"obj":"CC"},{"id":"T19741","span":{"begin":472,"end":474},"obj":","},{"id":"T19740","span":{"begin":464,"end":472},"obj":"NN"},{"id":"T19739","span":{"begin":461,"end":463},"obj":"IN"},{"id":"T19738","span":{"begin":451,"end":456},"obj":"JJ"},{"id":"T19737","span":{"begin":457,"end":460},"obj":"NN"},{"id":"T19736","span":{"begin":447,"end":450},"obj":"DT"},{"id":"T19735","span":{"begin":439,"end":446},"obj":"IN"},{"id":"T19734","span":{"begin":431,"end":438},"obj":"VBN"},{"id":"T19733","span":{"begin":429,"end":431},"obj":","},{"id":"T19732","span":{"begin":415,"end":416},"obj":"-RRB-"},{"id":"T19731","span":{"begin":414,"end":415},"obj":"CD"},{"id":"T19730","span":{"begin":407,"end":413},"obj":"NN"},{"id":"T19729","span":{"begin":406,"end":407},"obj":"-LRB-"},{"id":"T19728","span":{"begin":417,"end":422},"obj":"NN"},{"id":"T19727","span":{"begin":399,"end":405},"obj":"JJ"},{"id":"T19726","span":{"begin":423,"end":429},"obj":"NN"},{"id":"T19725","span":{"begin":397,"end":398},"obj":"DT"},{"id":"T19724","span":{"begin":391,"end":396},"obj":"VBG"},{"id":"T19723","span":{"begin":381,"end":390},"obj":"NNS"},{"id":"T19722","span":{"begin":372,"end":380},"obj":"JJ"},{"id":"T19721","span":{"begin":369,"end":371},"obj":"IN"},{"id":"T19720","span":{"begin":354,"end":358},"obj":"VBD"},{"id":"T19719","span":{"begin":340,"end":346},"obj":"NN"},{"id":"T19718","span":{"begin":347,"end":353},"obj":"NN"},{"id":"T19717","span":{"begin":336,"end":339},"obj":"DT"},{"id":"T19716","span":{"begin":333,"end":335},"obj":"IN"},{"id":"T19715","span":{"begin":322,"end":324},"obj":"NN"},{"id":"T19714","span":{"begin":316,"end":321},"obj":"CD"},{"id":"T19713","span":{"begin":325,"end":332},"obj":"NN"},{"id":"T19712","span":{"begin":314,"end":315},"obj":"DT"},{"id":"T19711","span":{"begin":310,"end":313},"obj":"CC"},{"id":"T19710","span":{"begin":297,"end":298},"obj":"HYPH"},{"id":"T19709","span":{"begin":298,"end":302},"obj":"NN"},{"id":"T19708","span":{"begin":293,"end":297},"obj":"JJ"},{"id":"T19707","span":{"begin":303,"end":309},"obj":"NN"},{"id":"T19706","span":{"begin":289,"end":292},"obj":"DT"},{"id":"T19705","span":{"begin":286,"end":288},"obj":"IN"},{"id":"T19704","span":{"begin":359,"end":368},"obj":"VBN"},{"id":"T19703","span":{"begin":275,"end":277},"obj":"NN"},{"id":"T19702","span":{"begin":269,"end":274},"obj":"CD"},{"id":"T19701","span":{"begin":278,"end":285},"obj":"NN"},{"id":"T19700","span":{"begin":267,"end":268},"obj":"DT"},{"id":"T19699","span":{"begin":266,"end":590},"obj":"sentence"},{"id":"T19698","span":{"begin":265,"end":266},"obj":"."},{"id":"T19697","span":{"begin":264,"end":265},"obj":"-RRB-"},{"id":"T19696","span":{"begin":263,"end":264},"obj":"CD"},{"id":"T19695","span":{"begin":256,"end":262},"obj":"NN"},{"id":"T19694","span":{"begin":255,"end":256},"obj":"-LRB-"},{"id":"T19693","span":{"begin":245,"end":254},"obj":"NNS"},{"id":"T19692","span":{"begin":241,"end":244},"obj":"CC"},{"id":"T19691","span":{"begin":233,"end":240},"obj":"NNS"},{"id":"T19690","span":{"begin":215,"end":232},"obj":"JJ"},{"id":"T19689","span":{"begin":210,"end":214},"obj":"IN"},{"id":"T19688","span":{"begin":201,"end":209},"obj":"NNS"},{"id":"T19687","span":{"begin":198,"end":200},"obj":"CC"},{"id":"T19686","span":{"begin":192,"end":197},"obj":"NNS"},{"id":"T19685","span":{"begin":189,"end":191},"obj":"NN"},{"id":"T19684","span":{"begin":178,"end":188},"obj":"VBG"},{"id":"T19683","span":{"begin":174,"end":177},"obj":"IN"},{"id":"T19682","span":{"begin":163,"end":173},"obj":"JJ"},{"id":"T19681","span":{"begin":159,"end":162},"obj":"VBD"},{"id":"T19680","span":{"begin":146,"end":149},"obj":"NN"},{"id":"T19679","span":{"begin":140,"end":141},"obj":"HYPH"},{"id":"T19678","span":{"begin":141,"end":145},"obj":"NN"},{"id":"T19677","span":{"begin":134,"end":140},"obj":"JJ"},{"id":"T19676","span":{"begin":150,"end":158},"obj":"NN"},{"id":"T19675","span":{"begin":132,"end":133},"obj":"DT"},{"id":"T19674","span":{"begin":131,"end":266},"obj":"sentence"},{"id":"T19673","span":{"begin":130,"end":131},"obj":"."},{"id":"T19672","span":{"begin":129,"end":130},"obj":"CD"},{"id":"T19671","span":{"begin":123,"end":128},"obj":"NN"},{"id":"T19670","span":{"begin":120,"end":122},"obj":"IN"},{"id":"T19669","span":{"begin":109,"end":112},"obj":"VBP"},{"id":"T19668","span":{"begin":85,"end":100},"obj":"NN"},{"id":"T19667","span":{"begin":101,"end":108},"obj":"NNS"},{"id":"T19666","span":{"begin":81,"end":84},"obj":"DT"},{"id":"T19665","span":{"begin":78,"end":80},"obj":"IN"},{"id":"T19664","span":{"begin":113,"end":119},"obj":"VBN"},{"id":"T19663","span":{"begin":68,"end":77},"obj":"NNS"},{"id":"T19662","span":{"begin":64,"end":67},"obj":"DT"},{"id":"T19661","span":{"begin":63,"end":131},"obj":"sentence"},{"id":"T19660","span":{"begin":62,"end":63},"obj":"."},{"id":"T19659","span":{"begin":59,"end":62},"obj":"NN"},{"id":"T19658","span":{"begin":51,"end":58},"obj":"JJ"},{"id":"T19657","span":{"begin":48,"end":50},"obj":"IN"},{"id":"T19656","span":{"begin":39,"end":47},"obj":"NN"},{"id":"T19655","span":{"begin":35,"end":38},"obj":"NN"},{"id":"T19654","span":{"begin":32,"end":34},"obj":"IN"},{"id":"T19653","span":{"begin":28,"end":31},"obj":"RP"},{"id":"T19652","span":{"begin":15,"end":19},"obj":"RB"},{"id":"T19651","span":{"begin":11,"end":14},"obj":"VBD"},{"id":"T19650","span":{"begin":20,"end":27},"obj":"VBN"},{"id":"T19649","span":{"begin":0,"end":10},"obj":"NN"},{"id":"T19817","span":{"begin":826,"end":827},"obj":"."},{"id":"T19816","span":{"begin":812,"end":821},"obj":"NN"},{"id":"T19815","span":{"begin":806,"end":811},"obj":"JJ"},{"id":"T19814","span":{"begin":799,"end":805},"obj":"NN"},{"id":"T19813","span":{"begin":795,"end":798},"obj":"CD"},{"id":"T19812","span":{"begin":822,"end":826},"obj":"NN"},{"id":"T19811","span":{"begin":793,"end":794},"obj":"DT"},{"id":"T19810","span":{"begin":788,"end":792},"obj":"IN"},{"id":"T19809","span":{"begin":786,"end":788},"obj":","},{"id":"T19808","span":{"begin":784,"end":786},"obj":"NN"},{"id":"T19807","span":{"begin":782,"end":784},"obj":"CD"},{"id":"T19806","span":{"begin":779,"end":781},"obj":"IN"},{"id":"T19805","span":{"begin":769,"end":778},"obj":"NN"},{"id":"T19804","span":{"begin":762,"end":768},"obj":"NN"},{"id":"T19793","span":{"begin":720,"end":722},"obj":"NN"},{"id":"T19792","span":{"begin":718,"end":720},"obj":"CD"},{"id":"T19791","span":{"begin":715,"end":717},"obj":"IN"},{"id":"T19790","span":{"begin":702,"end":714},"obj":"NN"},{"id":"T19789","span":{"begin":695,"end":701},"obj":"NN"},{"id":"T19788","span":{"begin":691,"end":694},"obj":"CD"},{"id":"T19787","span":{"begin":688,"end":690},"obj":"IN"},{"id":"T19786","span":{"begin":681,"end":687},"obj":"NNS"},{"id":"T19785","span":{"begin":678,"end":680},"obj":"CD"},{"id":"T19784","span":{"begin":676,"end":678},"obj":","},{"id":"T19783","span":{"begin":674,"end":676},"obj":"NN"},{"id":"T19782","span":{"begin":672,"end":674},"obj":"CD"},{"id":"T19781","span":{"begin":669,"end":671},"obj":"IN"},{"id":"T19780","span":{"begin":651,"end":663},"obj":"NN"},{"id":"T19779","span":{"begin":643,"end":650},"obj":"JJ"},{"id":"T19778","span":{"begin":636,"end":642},"obj":"NN"},{"id":"T19777","span":{"begin":631,"end":635},"obj":"CD"},{"id":"T19776","span":{"begin":664,"end":668},"obj":"NN"},{"id":"T19775","span":{"begin":629,"end":630},"obj":"DT"},{"id":"T19774","span":{"begin":620,"end":628},"obj":"VBD"},{"id":"T19773","span":{"begin":595,"end":608},"obj":"VBG"},{"id":"T19772","span":{"begin":609,"end":619},"obj":"NNS"},{"id":"T19771","span":{"begin":591,"end":594},"obj":"DT"},{"id":"T19770","span":{"begin":590,"end":827},"obj":"sentence"},{"id":"T19769","span":{"begin":589,"end":590},"obj":"."},{"id":"T19803","span":{"begin":758,"end":761},"obj":"CD"},{"id":"T19802","span":{"begin":754,"end":757},"obj":"CC"},{"id":"T19801","span":{"begin":752,"end":754},"obj":","},{"id":"T19800","span":{"begin":750,"end":752},"obj":"NN"},{"id":"T19799","span":{"begin":748,"end":750},"obj":"CD"},{"id":"T19798","span":{"begin":745,"end":747},"obj":"IN"},{"id":"T19797","span":{"begin":735,"end":744},"obj":"VBG"},{"id":"T19796","span":{"begin":728,"end":734},"obj":"NN"},{"id":"T19795","span":{"begin":724,"end":727},"obj":"CD"},{"id":"T19794","span":{"begin":722,"end":724},"obj":","}],"relations":[{"id":"R5578","pred":"nsubjpass","subj":"T19649","obj":"T19650"},{"id":"R5579","pred":"auxpass","subj":"T19651","obj":"T19650"},{"id":"R5580","pred":"advmod","subj":"T19652","obj":"T19650"},{"id":"R5581","pred":"prt","subj":"T19653","obj":"T19650"},{"id":"R5582","pred":"agent","subj":"T19654","obj":"T19650"},{"id":"R5583","pred":"compound","subj":"T19655","obj":"T19656"},{"id":"R5584","pred":"pobj","subj":"T19656","obj":"T19654"},{"id":"R5585","pred":"prep","subj":"T19657","obj":"T19656"},{"id":"R5586","pred":"amod","subj":"T19658","obj":"T19659"},{"id":"R5587","pred":"pobj","subj":"T19659","obj":"T19657"},{"id":"R5588","pred":"punct","subj":"T19660","obj":"T19650"},{"id":"R5589","pred":"det","subj":"T19662","obj":"T19663"},{"id":"R5590","pred":"nsubjpass","subj":"T19663","obj":"T19664"},{"id":"R5591","pred":"prep","subj":"T19665","obj":"T19663"},{"id":"R5592","pred":"det","subj":"T19666","obj":"T19667"},{"id":"R5593","pred":"pobj","subj":"T19667","obj":"T19665"},{"id":"R5594","pred":"compound","subj":"T19668","obj":"T19667"},{"id":"R5595","pred":"auxpass","subj":"T19669","obj":"T19664"},{"id":"R5596","pred":"prep","subj":"T19670","obj":"T19664"},{"id":"R5597","pred":"pobj","subj":"T19671","obj":"T19670"},{"id":"R5598","pred":"nummod","subj":"T19672","obj":"T19671"},{"id":"R5599","pred":"punct","subj":"T19673","obj":"T19664"},{"id":"R5600","pred":"det","subj":"T19675","obj":"T19676"},{"id":"R5601","pred":"nsubj","subj":"T19676","obj":"T19681"},{"id":"R5602","pred":"amod","subj":"T19677","obj":"T19678"},{"id":"R5603","pred":"compound","subj":"T19678","obj":"T19676"},{"id":"R5604","pred":"punct","subj":"T19679","obj":"T19678"},{"id":"R5605","pred":"compound","subj":"T19680","obj":"T19676"},{"id":"R5606","pred":"acomp","subj":"T19682","obj":"T19681"},{"id":"R5607","pred":"prep","subj":"T19683","obj":"T19682"},{"id":"R5608","pred":"pcomp","subj":"T19684","obj":"T19683"},{"id":"R5609","pred":"compound","subj":"T19685","obj":"T19686"},{"id":"R5610","pred":"dobj","subj":"T19686","obj":"T19684"},{"id":"R5611","pred":"cc","subj":"T19687","obj":"T19686"},{"id":"R5612","pred":"conj","subj":"T19688","obj":"T19686"},{"id":"R5613","pred":"prep","subj":"T19689","obj":"T19688"},{"id":"R5614","pred":"amod","subj":"T19690","obj":"T19691"},{"id":"R5615","pred":"pobj","subj":"T19691","obj":"T19689"},{"id":"R5616","pred":"cc","subj":"T19692","obj":"T19691"},{"id":"R5617","pred":"conj","subj":"T19693","obj":"T19691"},{"id":"R5618","pred":"punct","subj":"T19694","obj":"T19695"},{"id":"R5619","pred":"parataxis","subj":"T19695","obj":"T19684"},{"id":"R5620","pred":"nummod","subj":"T19696","obj":"T19695"},{"id":"R5621","pred":"punct","subj":"T19697","obj":"T19695"},{"id":"R5622","pred":"punct","subj":"T19698","obj":"T19681"},{"id":"R5623","pred":"det","subj":"T19700","obj":"T19701"},{"id":"R5624","pred":"nsubjpass","subj":"T19701","obj":"T19704"},{"id":"R5625","pred":"nummod","subj":"T19702","obj":"T19703"},{"id":"R5626","pred":"compound","subj":"T19703","obj":"T19701"},{"id":"R5627","pred":"prep","subj":"T19705","obj":"T19701"},{"id":"R5628","pred":"det","subj":"T19706","obj":"T19707"},{"id":"R5629","pred":"pobj","subj":"T19707","obj":"T19705"},{"id":"R5630","pred":"amod","subj":"T19708","obj":"T19709"},{"id":"R5631","pred":"compound","subj":"T19709","obj":"T19707"},{"id":"R5632","pred":"punct","subj":"T19710","obj":"T19709"},{"id":"R5633","pred":"cc","subj":"T19711","obj":"T19701"},{"id":"R5634","pred":"det","subj":"T19712","obj":"T19713"},{"id":"R5635","pred":"conj","subj":"T19713","obj":"T19701"},{"id":"R5636","pred":"nummod","subj":"T19714","obj":"T19715"},{"id":"R5637","pred":"compound","subj":"T19715","obj":"T19713"},{"id":"R5638","pred":"prep","subj":"T19716","obj":"T19713"},{"id":"R5639","pred":"det","subj":"T19717","obj":"T19718"},{"id":"R5640","pred":"pobj","subj":"T19718","obj":"T19716"},{"id":"R5641","pred":"compound","subj":"T19719","obj":"T19718"},{"id":"R5642","pred":"auxpass","subj":"T19720","obj":"T19704"},{"id":"R5643","pred":"prep","subj":"T19721","obj":"T19704"},{"id":"R5644","pred":"amod","subj":"T19722","obj":"T19723"},{"id":"R5645","pred":"pobj","subj":"T19723","obj":"T19721"},{"id":"R5646","pred":"advcl","subj":"T19724","obj":"T19704"},{"id":"R5647","pred":"det","subj":"T19725","obj":"T19726"},{"id":"R5648","pred":"dobj","subj":"T19726","obj":"T19724"},{"id":"R5649","pred":"amod","subj":"T19727","obj":"T19728"},{"id":"R5650","pred":"compound","subj":"T19728","obj":"T19726"},{"id":"R5651","pred":"punct","subj":"T19729","obj":"T19730"},{"id":"R5652","pred":"parataxis","subj":"T19730","obj":"T19728"},{"id":"R5653","pred":"nummod","subj":"T19731","obj":"T19730"},{"id":"R5654","pred":"punct","subj":"T19732","obj":"T19730"},{"id":"R5655","pred":"punct","subj":"T19733","obj":"T19726"},{"id":"R5656","pred":"acl","subj":"T19734","obj":"T19726"},{"id":"R5657","pred":"prep","subj":"T19735","obj":"T19734"},{"id":"R5658","pred":"det","subj":"T19736","obj":"T19737"},{"id":"R5659","pred":"pobj","subj":"T19737","obj":"T19735"},{"id":"R5660","pred":"amod","subj":"T19738","obj":"T19737"},{"id":"R5661","pred":"prep","subj":"T19739","obj":"T19737"},{"id":"R5662","pred":"pobj","subj":"T19740","obj":"T19739"},{"id":"R5663","pred":"punct","subj":"T19741","obj":"T19726"},{"id":"R5664","pred":"cc","subj":"T19742","obj":"T19726"},{"id":"R5665","pred":"amod","subj":"T19743","obj":"T19744"},{"id":"R5666","pred":"conj","subj":"T19744","obj":"T19726"},{"id":"R5667","pred":"compound","subj":"T19745","obj":"T19744"},{"id":"R5668","pred":"dep","subj":"T19746","obj":"T19747"},{"id":"R5669","pred":"relcl","subj":"T19747","obj":"T19744"},{"id":"R5670","pred":"prep","subj":"T19748","obj":"T19747"},{"id":"R5671","pred":"preconj","subj":"T19749","obj":"T19750"},{"id":"R5672","pred":"nmod","subj":"T19750","obj":"T19753"},{"id":"R5673","pred":"amod","subj":"T19751","obj":"T19750"},{"id":"R5674","pred":"punct","subj":"T19752","obj":"T19750"},{"id":"R5675","pred":"pobj","subj":"T19753","obj":"T19748"},{"id":"R5676","pred":"punct","subj":"T19754","obj":"T19755"},{"id":"R5677","pred":"parataxis","subj":"T19755","obj":"T19750"},{"id":"R5678","pred":"nummod","subj":"T19756","obj":"T19755"},{"id":"R5679","pred":"punct","subj":"T19757","obj":"T19755"},{"id":"R5680","pred":"cc","subj":"T19758","obj":"T19750"},{"id":"R5681","pred":"conj","subj":"T19759","obj":"T19750"},{"id":"R5682","pred":"punct","subj":"T19760","obj":"T19761"},{"id":"R5683","pred":"parataxis","subj":"T19761","obj":"T19759"},{"id":"R5684","pred":"compound","subj":"T19762","obj":"T19761"},{"id":"R5685","pred":"punct","subj":"T19763","obj":"T19761"},{"id":"R5686","pred":"punct","subj":"T19764","obj":"T19761"},{"id":"R5687","pred":"punct","subj":"T19765","obj":"T19766"},{"id":"R5688","pred":"parataxis","subj":"T19766","obj":"T19747"},{"id":"R5689","pred":"compound","subj":"T19767","obj":"T19766"},{"id":"R5690","pred":"punct","subj":"T19768","obj":"T19766"},{"id":"R5691","pred":"punct","subj":"T19769","obj":"T19704"},{"id":"R5692","pred":"det","subj":"T19771","obj":"T19772"},{"id":"R5693","pred":"nsubj","subj":"T19772","obj":"T19774"},{"id":"R5694","pred":"amod","subj":"T19773","obj":"T19772"},{"id":"R5695","pred":"det","subj":"T19775","obj":"T19776"},{"id":"R5696","pred":"dobj","subj":"T19776","obj":"T19774"},{"id":"R5697","pred":"nummod","subj":"T19777","obj":"T19778"},{"id":"R5698","pred":"nmod","subj":"T19778","obj":"T19776"},{"id":"R5699","pred":"amod","subj":"T19779","obj":"T19776"},{"id":"R5700","pred":"compound","subj":"T19780","obj":"T19776"},{"id":"R5701","pred":"prep","subj":"T19781","obj":"T19776"},{"id":"R5702","pred":"nummod","subj":"T19782","obj":"T19783"},{"id":"R5703","pred":"pobj","subj":"T19783","obj":"T19781"},{"id":"R5704","pred":"punct","subj":"T19784","obj":"T19776"},{"id":"R5705","pred":"nummod","subj":"T19785","obj":"T19786"},{"id":"R5706","pred":"conj","subj":"T19786","obj":"T19776"},{"id":"R5707","pred":"prep","subj":"T19787","obj":"T19786"},{"id":"R5716","pred":"conj","subj":"T19796","obj":"T19786"},{"id":"R5717","pred":"amod","subj":"T19797","obj":"T19796"},{"id":"R5718","pred":"prep","subj":"T19798","obj":"T19796"},{"id":"R5719","pred":"nummod","subj":"T19799","obj":"T19800"},{"id":"R5720","pred":"pobj","subj":"T19800","obj":"T19798"},{"id":"R5721","pred":"punct","subj":"T19801","obj":"T19796"},{"id":"R5722","pred":"cc","subj":"T19802","obj":"T19796"},{"id":"R5723","pred":"nummod","subj":"T19803","obj":"T19804"},{"id":"R5724","pred":"compound","subj":"T19804","obj":"T19805"},{"id":"R5725","pred":"conj","subj":"T19805","obj":"T19796"},{"id":"R5726","pred":"prep","subj":"T19806","obj":"T19805"},{"id":"R5727","pred":"nummod","subj":"T19807","obj":"T19808"},{"id":"R5728","pred":"pobj","subj":"T19808","obj":"T19806"},{"id":"R5729","pred":"punct","subj":"T19809","obj":"T19776"},{"id":"R5730","pred":"prep","subj":"T19810","obj":"T19776"},{"id":"R5731","pred":"det","subj":"T19811","obj":"T19812"},{"id":"R5732","pred":"pobj","subj":"T19812","obj":"T19810"},{"id":"R5733","pred":"nummod","subj":"T19813","obj":"T19814"},{"id":"R5734","pred":"nmod","subj":"T19814","obj":"T19812"},{"id":"R5735","pred":"amod","subj":"T19815","obj":"T19812"},{"id":"R5736","pred":"compound","subj":"T19816","obj":"T19812"},{"id":"R5737","pred":"punct","subj":"T19817","obj":"T19774"},{"id":"R5708","pred":"nummod","subj":"T19788","obj":"T19789"},{"id":"R5709","pred":"compound","subj":"T19789","obj":"T19790"},{"id":"R5710","pred":"pobj","subj":"T19790","obj":"T19787"},{"id":"R5711","pred":"prep","subj":"T19791","obj":"T19786"},{"id":"R5712","pred":"nummod","subj":"T19792","obj":"T19793"},{"id":"R5713","pred":"pobj","subj":"T19793","obj":"T19791"},{"id":"R5714","pred":"punct","subj":"T19794","obj":"T19786"},{"id":"R5715","pred":"nummod","subj":"T19795","obj":"T19796"}],"attributes":[{"subj":"T19768","pred":"source","obj":"craft-sa-dev"},{"subj":"T19767","pred":"source","obj":"craft-sa-dev"},{"subj":"T19766","pred":"source","obj":"craft-sa-dev"},{"subj":"T19765","pred":"source","obj":"craft-sa-dev"},{"subj":"T19764","pred":"source","obj":"craft-sa-dev"},{"subj":"T19763","pred":"source","obj":"craft-sa-dev"},{"subj":"T19762","pred":"source","obj":"craft-sa-dev"},{"subj":"T19761","pred":"source","obj":"craft-sa-dev"},{"subj":"T19760","pred":"source","obj":"craft-sa-dev"},{"subj":"T19759","pred":"source","obj":"craft-sa-dev"},{"subj":"T19758","pred":"source","obj":"craft-sa-dev"},{"subj":"T19757","pred":"source","obj":"craft-sa-dev"},{"subj":"T19756","pred":"source","obj":"craft-sa-dev"},{"subj":"T19755","pred":"source","obj":"craft-sa-dev"},{"subj":"T19754","pred":"source","obj":"craft-sa-dev"},{"subj":"T19753","pred":"source","obj":"craft-sa-dev"},{"subj":"T19752","pred":"source","obj":"craft-sa-dev"},{"subj":"T19751","pred":"source","obj":"craft-sa-dev"},{"subj":"T19750","pred":"source","obj":"craft-sa-dev"},{"subj":"T19749","pred":"source","obj":"craft-sa-dev"},{"subj":"T19748","pred":"source","obj":"craft-sa-dev"},{"subj":"T19747","pred":"source","obj":"craft-sa-dev"},{"subj":"T19746","pred":"source","obj":"craft-sa-dev"},{"subj":"T19745","pred":"source","obj":"craft-sa-dev"},{"subj":"T19744","pred":"source","obj":"craft-sa-dev"},{"subj":"T19743","pred":"source","obj":"craft-sa-dev"},{"subj":"T19742","pred":"source","obj":"craft-sa-dev"},{"subj":"T19741","pred":"source","obj":"craft-sa-dev"},{"subj":"T19740","pred":"source","obj":"craft-sa-dev"},{"subj":"T19739","pred":"source","obj":"craft-sa-dev"},{"subj":"T19738","pred":"source","obj":"craft-sa-dev"},{"subj":"T19737","pred":"source","obj":"craft-sa-dev"},{"subj":"T19736","pred":"source","obj":"craft-sa-dev"},{"subj":"T19735","pred":"source","obj":"craft-sa-dev"},{"subj":"T19734","pred":"source","obj":"craft-sa-dev"},{"subj":"T19733","pred":"source","obj":"craft-sa-dev"},{"subj":"T19732","pred":"source","obj":"craft-sa-dev"},{"subj":"T19731","pred":"source","obj":"craft-sa-dev"},{"subj":"T19730","pred":"source","obj":"craft-sa-dev"},{"subj":"T19729","pred":"source","obj":"craft-sa-dev"},{"subj":"T19728","pred":"source","obj":"craft-sa-dev"},{"subj":"T19727","pred":"source","obj":"craft-sa-dev"},{"subj":"T19726","pred":"source","obj":"craft-sa-dev"},{"subj":"T19725","pred":"source","obj":"craft-sa-dev"},{"subj":"T19724","pred":"source","obj":"craft-sa-dev"},{"subj":"T19723","pred":"source","obj":"craft-sa-dev"},{"subj":"T19722","pred":"source","obj":"craft-sa-dev"},{"subj":"T19721","pred":"source","obj":"craft-sa-dev"},{"subj":"T19720","pred":"source","obj":"craft-sa-dev"},{"subj":"T19719","pred":"source","obj":"craft-sa-dev"},{"subj":"T19718","pred":"source","obj":"craft-sa-dev"},{"subj":"T19717","pred":"source","obj":"craft-sa-dev"},{"subj":"T19716","pred":"source","obj":"craft-sa-dev"},{"subj":"T19715","pred":"source","obj":"craft-sa-dev"},{"subj":"T19714","pred":"source","obj":"craft-sa-dev"},{"subj":"T19713","pred":"source","obj":"craft-sa-dev"},{"subj":"T19712","pred":"source","obj":"craft-sa-dev"},{"subj":"T19711","pred":"source","obj":"craft-sa-dev"},{"subj":"T19710","pred":"source","obj":"craft-sa-dev"},{"subj":"T19709","pred":"source","obj":"craft-sa-dev"},{"subj":"T19708","pred":"source","obj":"craft-sa-dev"},{"subj":"T19707","pred":"source","obj":"craft-sa-dev"},{"subj":"T19706","pred":"source","obj":"craft-sa-dev"},{"subj":"T19705","pred":"source","obj":"craft-sa-dev"},{"subj":"T19704","pred":"source","obj":"craft-sa-dev"},{"subj":"T19703","pred":"source","obj":"craft-sa-dev"},{"subj":"T19702","pred":"source","obj":"craft-sa-dev"},{"subj":"T19701","pred":"source","obj":"craft-sa-dev"},{"subj":"T19700","pred":"source","obj":"craft-sa-dev"},{"subj":"T19699","pred":"source","obj":"craft-sa-dev"},{"subj":"T19698","pred":"source","obj":"craft-sa-dev"},{"subj":"T19697","pred":"source","obj":"craft-sa-dev"},{"subj":"T19696","pred":"source","obj":"craft-sa-dev"},{"subj":"T19695","pred":"source","obj":"craft-sa-dev"},{"subj":"T19694","pred":"source","obj":"craft-sa-dev"},{"subj":"T19693","pred":"source","obj":"craft-sa-dev"},{"subj":"T19692","pred":"source","obj":"craft-sa-dev"},{"subj":"T19691","pred":"source","obj":"craft-sa-dev"},{"subj":"T19690","pred":"source","obj":"craft-sa-dev"},{"subj":"T19689","pred":"source","obj":"craft-sa-dev"},{"subj":"T19688","pred":"source","obj":"craft-sa-dev"},{"subj":"T19687","pred":"source","obj":"craft-sa-dev"},{"subj":"T19686","pred":"source","obj":"craft-sa-dev"},{"subj":"T19685","pred":"source","obj":"craft-sa-dev"},{"subj":"T19684","pred":"source","obj":"craft-sa-dev"},{"subj":"T19683","pred":"source","obj":"craft-sa-dev"},{"subj":"T19682","pred":"source","obj":"craft-sa-dev"},{"subj":"T19681","pred":"source","obj":"craft-sa-dev"},{"subj":"T19680","pred":"source","obj":"craft-sa-dev"},{"subj":"T19679","pred":"source","obj":"craft-sa-dev"},{"subj":"T19678","pred":"source","obj":"craft-sa-dev"},{"subj":"T19677","pred":"source","obj":"craft-sa-dev"},{"subj":"T19676","pred":"source","obj":"craft-sa-dev"},{"subj":"T19675","pred":"source","obj":"craft-sa-dev"},{"subj":"T19674","pred":"source","obj":"craft-sa-dev"},{"subj":"T19673","pred":"source","obj":"craft-sa-dev"},{"subj":"T19672","pred":"source","obj":"craft-sa-dev"},{"subj":"T19671","pred":"source","obj":"craft-sa-dev"},{"subj":"T19670","pred":"source","obj":"craft-sa-dev"},{"subj":"T19669","pred":"source","obj":"craft-sa-dev"},{"subj":"T19668","pred":"source","obj":"craft-sa-dev"},{"subj":"T19667","pred":"source","obj":"craft-sa-dev"},{"subj":"T19666","pred":"source","obj":"craft-sa-dev"},{"subj":"T19665","pred":"source","obj":"craft-sa-dev"},{"subj":"T19664","pred":"source","obj":"craft-sa-dev"},{"subj":"T19663","pred":"source","obj":"craft-sa-dev"},{"subj":"T19662","pred":"source","obj":"craft-sa-dev"},{"subj":"T19661","pred":"source","obj":"craft-sa-dev"},{"subj":"T19660","pred":"source","obj":"craft-sa-dev"},{"subj":"T19659","pred":"source","obj":"craft-sa-dev"},{"subj":"T19658","pred":"source","obj":"craft-sa-dev"},{"subj":"T19657","pred":"source","obj":"craft-sa-dev"},{"subj":"T19656","pred":"source","obj":"craft-sa-dev"},{"subj":"T19655","pred":"source","obj":"craft-sa-dev"},{"subj":"T19654","pred":"source","obj":"craft-sa-dev"},{"subj":"T19653","pred":"source","obj":"craft-sa-dev"},{"subj":"T19652","pred":"source","obj":"craft-sa-dev"},{"subj":"T19651","pred":"source","obj":"craft-sa-dev"},{"subj":"T19650","pred":"source","obj":"craft-sa-dev"},{"subj":"T19649","pred":"source","obj":"craft-sa-dev"},{"subj":"T19817","pred":"source","obj":"craft-sa-dev"},{"subj":"T19816","pred":"source","obj":"craft-sa-dev"},{"subj":"T19815","pred":"source","obj":"craft-sa-dev"},{"subj":"T19814","pred":"source","obj":"craft-sa-dev"},{"subj":"T19813","pred":"source","obj":"craft-sa-dev"},{"subj":"T19812","pred":"source","obj":"craft-sa-dev"},{"subj":"T19811","pred":"source","obj":"craft-sa-dev"},{"subj":"T19810","pred":"source","obj":"craft-sa-dev"},{"subj":"T19809","pred":"source","obj":"craft-sa-dev"},{"subj":"T19808","pred":"source","obj":"craft-sa-dev"},{"subj":"T19807","pred":"source","obj":"craft-sa-dev"},{"subj":"T19806","pred":"source","obj":"craft-sa-dev"},{"subj":"T19805","pred":"source","obj":"craft-sa-dev"},{"subj":"T19804","pred":"source","obj":"craft-sa-dev"},{"subj":"T19793","pred":"source","obj":"craft-sa-dev"},{"subj":"T19792","pred":"source","obj":"craft-sa-dev"},{"subj":"T19791","pred":"source","obj":"craft-sa-dev"},{"subj":"T19790","pred":"source","obj":"craft-sa-dev"},{"subj":"T19789","pred":"source","obj":"craft-sa-dev"},{"subj":"T19788","pred":"source","obj":"craft-sa-dev"},{"subj":"T19787","pred":"source","obj":"craft-sa-dev"},{"subj":"T19786","pred":"source","obj":"craft-sa-dev"},{"subj":"T19785","pred":"source","obj":"craft-sa-dev"},{"subj":"T19784","pred":"source","obj":"craft-sa-dev"},{"subj":"T19783","pred":"source","obj":"craft-sa-dev"},{"subj":"T19782","pred":"source","obj":"craft-sa-dev"},{"subj":"T19781","pred":"source","obj":"craft-sa-dev"},{"subj":"T19780","pred":"source","obj":"craft-sa-dev"},{"subj":"T19779","pred":"source","obj":"craft-sa-dev"},{"subj":"T19778","pred":"source","obj":"craft-sa-dev"},{"subj":"T19777","pred":"source","obj":"craft-sa-dev"},{"subj":"T19776","pred":"source","obj":"craft-sa-dev"},{"subj":"T19775","pred":"source","obj":"craft-sa-dev"},{"subj":"T19774","pred":"source","obj":"craft-sa-dev"},{"subj":"T19773","pred":"source","obj":"craft-sa-dev"},{"subj":"T19772","pred":"source","obj":"craft-sa-dev"},{"subj":"T19771","pred":"source","obj":"craft-sa-dev"},{"subj":"T19770","pred":"source","obj":"craft-sa-dev"},{"subj":"T19769","pred":"source","obj":"craft-sa-dev"},{"subj":"T19803","pred":"source","obj":"craft-sa-dev"},{"subj":"T19802","pred":"source","obj":"craft-sa-dev"},{"subj":"T19801","pred":"source","obj":"craft-sa-dev"},{"subj":"T19800","pred":"source","obj":"craft-sa-dev"},{"subj":"T19799","pred":"source","obj":"craft-sa-dev"},{"subj":"T19798","pred":"source","obj":"craft-sa-dev"},{"subj":"T19797","pred":"source","obj":"craft-sa-dev"},{"subj":"T19796","pred":"source","obj":"craft-sa-dev"},{"subj":"T19795","pred":"source","obj":"craft-sa-dev"},{"subj":"T19794","pred":"source","obj":"craft-sa-dev"}]},{"project":"craft-ca-core-dev","denotations":[{"id":"T19358","span":{"begin":407,"end":413},"obj":"SO:0000188"},{"id":"T19357","span":{"begin":347,"end":353},"obj":"SO:0001023"},{"id":"T19356","span":{"begin":322,"end":324},"obj":"SO:0000028"},{"id":"T19355","span":{"begin":303,"end":309},"obj":"SO:0001023"},{"id":"T19354","span":{"begin":275,"end":277},"obj":"SO:0000028"},{"id":"T19353","span":{"begin":233,"end":240},"obj":"UBERON:0000922"},{"id":"T19352","span":{"begin":220,"end":232},"obj":"GO:0007566"},{"id":"T19351","span":{"begin":189,"end":197},"obj":"CL:0002322"},{"id":"T19350","span":{"begin":101,"end":108},"obj":"SO:0000112"},{"id":"T19349","span":{"begin":51,"end":58},"obj":"SO:0001026"},{"id":"T19364","span":{"begin":735,"end":744},"obj":"GO:0097617"},{"id":"T19363","span":{"begin":543,"end":547},"obj":"SO:0000147"},{"id":"T19362","span":{"begin":511,"end":521},"obj":"GO:0097617"},{"id":"T19361","span":{"begin":497,"end":504},"obj":"SO:0000112"},{"id":"T19360","span":{"begin":487,"end":496},"obj":"SO:0000077"},{"id":"T19359","span":{"begin":423,"end":429},"obj":"SO:0000112"}],"attributes":[{"subj":"T19358","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19357","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19356","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19355","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19354","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19353","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19352","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19351","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19350","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19349","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19364","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19363","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19362","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19361","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19360","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T19359","pred":"source","obj":"craft-ca-core-dev"}]},{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T19462","span":{"begin":735,"end":744},"obj":"GO:0097617"},{"id":"T19461","span":{"begin":702,"end":714},"obj":"GO_EXT:macromolecule_denaturation"},{"id":"T19460","span":{"begin":651,"end":663},"obj":"GO_EXT:macromolecule_denaturation"},{"id":"T19459","span":{"begin":571,"end":579},"obj":"SO_EXT:biological_sequence"},{"id":"T19458","span":{"begin":566,"end":569},"obj":"CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase"},{"id":"T19457","span":{"begin":562,"end":565},"obj":"GO_EXT:phosphoglycerate_kinase"},{"id":"T19456","span":{"begin":554,"end":560},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T19455","span":{"begin":543,"end":547},"obj":"SO_EXT:0000147"},{"id":"T19454","span":{"begin":532,"end":541},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T19453","span":{"begin":511,"end":521},"obj":"GO:0097617"},{"id":"T19452","span":{"begin":497,"end":504},"obj":"SO_EXT:0000112"},{"id":"T19451","span":{"begin":487,"end":496},"obj":"SO:0000077"},{"id":"T19450","span":{"begin":423,"end":429},"obj":"SO_EXT:0000112"},{"id":"T19449","span":{"begin":407,"end":413},"obj":"SO_EXT:0000188"},{"id":"T19448","span":{"begin":347,"end":353},"obj":"SO_EXT:0001023"},{"id":"T19447","span":{"begin":340,"end":346},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T19446","span":{"begin":322,"end":324},"obj":"SO_EXT:0000028"},{"id":"T19445","span":{"begin":303,"end":309},"obj":"SO_EXT:0001023"},{"id":"T19444","span":{"begin":293,"end":302},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T19443","span":{"begin":275,"end":277},"obj":"SO_EXT:0000028"},{"id":"T19442","span":{"begin":233,"end":240},"obj":"UBERON:0000922"},{"id":"T19441","span":{"begin":220,"end":232},"obj":"GO:0007566"},{"id":"T19440","span":{"begin":192,"end":197},"obj":"CL_GO_EXT:cell"},{"id":"T19439","span":{"begin":189,"end":197},"obj":"CL:0002322"},{"id":"T19438","span":{"begin":101,"end":108},"obj":"SO_EXT:0000112"},{"id":"T19437","span":{"begin":85,"end":100},"obj":"CHEBI_SO_EXT:oligonucleotide"},{"id":"T19436","span":{"begin":68,"end":77},"obj":"SO_EXT:biological_sequence"},{"id":"T19435","span":{"begin":59,"end":62},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T19434","span":{"begin":51,"end":62},"obj":"SO_EXT:genomic_DNA"}],"attributes":[{"subj":"T19462","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19461","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19460","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19459","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19458","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19457","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19456","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19455","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19454","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19453","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19452","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19451","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19450","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19449","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19448","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19447","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19446","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19445","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19444","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19443","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19442","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19441","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19440","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19439","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19438","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19437","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19436","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19435","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T19434","pred":"source","obj":"craft-ca-core-ex-dev"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"craft-sa-dev","color":"#93c2ec","default":true},{"id":"craft-ca-core-dev","color":"#dcec93"},{"id":"craft-ca-core-ex-dev","color":"#e293ec"}]}]}}