PMC:1359074 / 40246-40767 JSONTXT

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protein extract and in vitro translation of Sox6.\nNuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States). The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15]. The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega). A vector without the Sox6 coding sequence was also translated as a negative control."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T19138","span":{"begin":458,"end":462},"obj":"Protein"},{"id":"T19137","span":{"begin":250,"end":252},"obj":"Protein"},{"id":"T19136","span":{"begin":240,"end":245},"obj":"Protein"},{"id":"T19135","span":{"begin":183,"end":187},"obj":"Protein"},{"id":"T19134","span":{"begin":52,"end":56},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Nuclear protein extract and in vitro translation of Sox6.\nNuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States). The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15]. The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega). A vector without the Sox6 coding sequence was also translated as a negative control."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T19243","span":{"begin":458,"end":462},"obj":"http://www.uniprot.org/uniprot/P35712"},{"id":"T19242","span":{"begin":183,"end":187},"obj":"http://www.uniprot.org/uniprot/P35712"},{"id":"T19241","span":{"begin":52,"end":56},"obj":"http://www.uniprot.org/uniprot/P35712"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Nuclear protein extract and in vitro translation of Sox6.\nNuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States). The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15]. The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega). A vector without the Sox6 coding sequence was also translated as a negative control."}

{"project":"GO-BP","denotations":[{"id":"T19128","span":{"begin":392,"end":405},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T19127","span":{"begin":488,"end":498},"obj":"http://purl.obolibrary.org/obo/GO_0006412"},{"id":"T19126","span":{"begin":285,"end":296},"obj":"http://purl.obolibrary.org/obo/GO_0006412"},{"id":"T19125","span":{"begin":197,"end":208},"obj":"http://purl.obolibrary.org/obo/GO_0006412"},{"id":"T19124","span":{"begin":37,"end":48},"obj":"http://purl.obolibrary.org/obo/GO_0006412"}],"text":"Nuclear protein extract and in vitro translation of Sox6.\nNuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States). The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15]. The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega). A vector without the Sox6 coding sequence was also translated as a negative control."}

{"project":"GO-CC","denotations":[{"id":"T19139","span":{"begin":98,"end":103},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Nuclear protein extract and in vitro translation of Sox6.\nNuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States). The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15]. The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega). A vector without the Sox6 coding sequence was also translated as a negative control."}

{"project":"sentences","denotations":[{"id":"T19123","span":{"begin":437,"end":521},"obj":"Sentence"},{"id":"T19122","span":{"begin":281,"end":436},"obj":"Sentence"},{"id":"T19121","span":{"begin":179,"end":280},"obj":"Sentence"},{"id":"T19120","span":{"begin":58,"end":178},"obj":"Sentence"},{"id":"T19119","span":{"begin":0,"end":57},"obj":"Sentence"},{"id":"T307","span":{"begin":0,"end":57},"obj":"Sentence"},{"id":"T308","span":{"begin":58,"end":178},"obj":"Sentence"},{"id":"T309","span":{"begin":179,"end":280},"obj":"Sentence"},{"id":"T310","span":{"begin":281,"end":436},"obj":"Sentence"},{"id":"T311","span":{"begin":437,"end":521},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Nuclear protein extract and in vitro translation of Sox6.\nNuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States). The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15]. The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega). A vector without the Sox6 coding sequence was also translated as a negative control."}

{"project":"simple1","denotations":[{"id":"T19149","span":{"begin":458,"end":462},"obj":"Protein"},{"id":"T19148","span":{"begin":250,"end":252},"obj":"Protein"},{"id":"T19147","span":{"begin":240,"end":245},"obj":"Protein"},{"id":"T19146","span":{"begin":183,"end":187},"obj":"Protein"},{"id":"T19145","span":{"begin":52,"end":56},"obj":"Protein"}],"text":"Nuclear protein extract and in vitro translation of Sox6.\nNuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States). The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15]. The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega). A vector without the Sox6 coding sequence was also translated as a negative control."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T19384","span":{"begin":209,"end":219},"obj":"Gene_expression"},{"id":"T19383","span":{"begin":458,"end":462},"obj":"Protein"},{"id":"T19382","span":{"begin":250,"end":252},"obj":"Protein"},{"id":"T19381","span":{"begin":240,"end":245},"obj":"Protein"},{"id":"T19380","span":{"begin":183,"end":187},"obj":"Protein"},{"id":"T19379","span":{"begin":52,"end":56},"obj":"Protein"}],"relations":[{"id":"R13771","pred":"themeOf","subj":"T19380","obj":"T19384"}],"text":"Nuclear protein extract and in vitro translation of Sox6.\nNuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States). The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15]. The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega). A vector without the Sox6 coding sequence was also translated as a negative control."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T19249","span":{"begin":209,"end":219},"obj":"Gene_expression"},{"id":"T19248","span":{"begin":458,"end":462},"obj":"Protein"},{"id":"T19247","span":{"begin":250,"end":252},"obj":"Protein"},{"id":"T19246","span":{"begin":240,"end":245},"obj":"Protein"},{"id":"T19245","span":{"begin":183,"end":187},"obj":"Protein"},{"id":"T19244","span":{"begin":52,"end":56},"obj":"Protein"}],"relations":[{"id":"R13668","pred":"themeOf","subj":"T19245","obj":"T19249"}],"text":"Nuclear protein extract and in vitro translation of Sox6.\nNuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States). The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15]. The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega). A vector without the Sox6 coding sequence was also translated as a negative control."}

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