PMC:1359074 / 33632-44136 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
16462943-1737096-85761022	428-430	1737096	denotes	50
16462943-2748594-85761023	868-870	2748594	denotes	31
16462943-14530442-85761024	1669-1671	14530442	denotes	15
16462943-12677004-85761025	1846-1848	12677004	denotes	32
16462943-14654707-85761026	2410-2412	14654707	denotes	51
16462943-8562047-85761027	3958-3960	8562047	denotes	52
16462943-14530442-85761028	6890-6892	14530442	denotes	15
16462943-11818535-85761029	7265-7266	11818535	denotes	7
16462943-15302897-85761030	9042-9044	15302897	denotes	53
T43787	428-430	1737096	denotes	50
T59528	868-870	2748594	denotes	31
T89386	1669-1671	14530442	denotes	15
T39276	1846-1848	12677004	denotes	32
T25296	2410-2412	14654707	denotes	51
T83427	3958-3960	8562047	denotes	52
T81428	6890-6892	14530442	denotes	15
T70190	7265-7266	11818535	denotes	7
T20377	9042-9044	15302897	denotes	53

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T19941	8875-8915	NN	denotes	5′AATGCAGAACAAAGGGTCAGAtgagTGTCTGCGAAG3′
T20665	10500-10503	NN	denotes	min
T20664	10498-10499	CD	denotes	5
T20663	10494-10497	IN	denotes	for
T20662	10491-10493	NN	denotes	°C
T20661	10488-10490	CD	denotes	72
T20660	10485-10487	IN	denotes	at
T20659	10475-10484	NN	denotes	extension
T20658	10469-10474	JJ	denotes	final
T20657	10467-10468	DT	denotes	a
T20656	10462-10466	IN	denotes	with
T20655	10455-10461	VB	denotes	ending
T20654	10453-10454	-COMMA-	denotes	,
T20653	10452-10453	NN	denotes	s
T20652	10449-10451	CD	denotes	45
T20651	10445-10448	IN	denotes	for
T20650	10442-10444	NN	denotes	°C
T20649	10439-10441	CD	denotes	72
T20648	10435-10438	CC	denotes	and
T20647	10433-10434	-COMMA-	denotes	,
T20646	10432-10433	NN	denotes	s
T20645	10429-10431	CD	denotes	30
T20644	10425-10428	IN	denotes	for
T20643	10422-10424	NN	denotes	°C
T20642	10419-10421	CD	denotes	60
T20641	10417-10418	-COMMA-	denotes	,
T20640	10416-10417	NN	denotes	s
T20639	10413-10415	CD	denotes	30
T20638	10409-10412	IN	denotes	for
T20637	10406-10408	NN	denotes	°C
T20636	10403-10405	CD	denotes	95
T20635	10400-10402	IN	denotes	at
T20634	10393-10399	NN	denotes	cycles
T20633	10390-10392	CD	denotes	30
T20632	10387-10389	IN	denotes	by
T20631	10378-10386	VB	denotes	followed
T20630	10374-10377	NN	denotes	min
T20629	10371-10373	CD	denotes	15
T20628	10367-10370	IN	denotes	for
T20627	10364-10366	NN	denotes	°C
T20626	10361-10363	CD	denotes	95
T20625	10359-10360	-COLON-	denotes	:
T20624	10349-10359	NN	denotes	conditions
T20623	10339-10348	JJ	denotes	following
T20622	10335-10338	DT	denotes	the
T20621	10329-10334	IN	denotes	under
T20620	10319-10328	VB	denotes	performed
T20619	10315-10318	VB	denotes	was
T20618	10311-10314	NN	denotes	PCR
T20617	10308-10309	-RRB-	denotes	)
T20616	10284-10308	NN	denotes	5′GCTTCACCACCAACCTCTTC3′
T20615	10282-10283	-COMMA-	denotes	,
T20614	10275-10282	NN	denotes	MHB1689
T20613	10271-10274	CC	denotes	and
T20612	10269-10270	-COLON-	denotes	;
T20611	10245-10269	NN	denotes	5′CGAAGAATAAAAGGCCACCA3′
T20610	10243-10244	-COMMA-	denotes	,
T20609	10236-10243	NN	denotes	MHB1688
T20608	10228-10235	NN	denotes	primers
T20607	10227-10228	-LRB-	denotes	(
T20606	10218-10226	NN	denotes	promoter
T20605	10215-10217	JJ	denotes	ɛy
T20604	10211-10214	DT	denotes	the
T20603	10208-10210	IN	denotes	of
T20602	10203-10207	CD	denotes	+140
T20601	10200-10202	TO	denotes	to
T20600	10196-10199	CD	denotes	−31
T20599	10184-10195	NN	denotes	nucleotides
T20598	10181-10183	TO	denotes	to
T20597	10167-10180	VB	denotes	corresponding
T20596	10165-10166	-COMMA-	denotes	,
T20595	10157-10165	NN	denotes	amplicon
T20594	10150-10156	JJ	denotes	172-bp
T20593	10148-10149	DT	denotes	a
T20592	10140-10147	VB	denotes	yielded
T20591	10136-10139	CC	denotes	and
T20590	10126-10135	VB	denotes	performed
T20589	10122-10125	VB	denotes	was
T20588	10113-10121	NN	denotes	promoter
T20587	10110-10112	JJ	denotes	ɛy
T20586	10106-10109	DT	denotes	the
T20585	10103-10105	IN	denotes	of
T20584	10089-10102	NN	denotes	amplification
T20583	10085-10088	NN	denotes	PCR
T20582	10075-10083	NN	denotes	reaction
T20581	10071-10074	NN	denotes	PCR
T20580	10067-10070	DT	denotes	the
T20579	10064-10066	IN	denotes	in
T20578	10055-10063	NN	denotes	template
T20577	10053-10054	DT	denotes	a
T20576	10050-10052	IN	denotes	as
T20575	10045-10049	VB	denotes	used
T20574	10041-10044	VB	denotes	was
T20573	10037-10040	NN	denotes	DNA
T20572	10018-10036	VB	denotes	immunoprecipitated
T20571	10015-10017	CC	denotes	or
T20570	10011-10014	NN	denotes	DNA
T20569	10005-10010	NN	denotes	Input
T20568	10002-10003	NN	denotes	h
T20567	10000-10001	CD	denotes	4
T20566	9996-9999	IN	denotes	for
T20565	9993-9995	NN	denotes	°C
T20564	9990-9992	CD	denotes	65
T20563	9987-9989	IN	denotes	at
T20562	9982-9986	NN	denotes	NaCl
T20561	9980-9981	NN	denotes	M
T20560	9978-9979	CD	denotes	5
T20559	9973-9977	IN	denotes	with
T20558	9964-9972	VB	denotes	reversed
T20557	9959-9963	VB	denotes	were
T20556	9947-9958	NN	denotes	cross-links
T20555	9939-9946	NN	denotes	protein
T20554	9935-9938	NN	denotes	DNA
T20553	9931-9934	DT	denotes	the
T20552	9927-9930	CC	denotes	and
T20551	9925-9926	-COMMA-	denotes	,
T20550	9919-9925	VB	denotes	eluted
T20549	9914-9918	VB	denotes	were
T20548	9904-9913	NN	denotes	complexes
T20547	9885-9903	NN	denotes	chromatin-antibody
T20546	9881-9884	DT	denotes	The
T20545	9871-9879	NN	denotes	controls
T20544	9862-9870	JJ	denotes	negative
T20543	9859-9861	IN	denotes	as
T20542	9854-9858	VB	denotes	used
T20541	9849-9853	VB	denotes	were
T20540	9845-9848	CD	denotes	two
T20539	9840-9844	JJ	denotes	last
T20538	9836-9839	DT	denotes	The
T20537	9832-9835	NNP	denotes	Ab.
T20536	9824-9831	IN	denotes	without
T20535	9817-9823	NN	denotes	sample
T20534	9811-9816	JJ	denotes	third
T20533	9807-9810	DT	denotes	the
T20532	9803-9806	CC	denotes	and
T20531	9801-9802	-COMMA-	denotes	,
T20530	9798-9801	NN	denotes	IgG
T20529	9791-9797	NN	denotes	rabbit
T20528	9784-9790	JJ	denotes	normal
T20527	9779-9783	IN	denotes	with
T20526	9771-9778	VB	denotes	treated
T20525	9764-9770	JJ	denotes	second
T20524	9762-9763	DT	denotes	a
T20523	9760-9761	-COMMA-	denotes	,
T20522	9751-9760	NN	denotes	anti-Sox6
T20521	9746-9750	IN	denotes	with
T20520	9738-9745	VB	denotes	treated
T20519	9731-9737	NN	denotes	sample
T20518	9727-9730	CD	denotes	one
T20517	9725-9726	-COLON-	denotes	:
T20516	9719-9725	NN	denotes	thirds
T20515	9714-9718	IN	denotes	into
T20514	9708-9713	VB	denotes	split
T20513	9703-9707	VB	denotes	were
T20512	9695-9702	NN	denotes	samples
T20511	9691-9694	DT	denotes	the
T20510	9689-9690	-COMMA-	denotes	,
T20509	9678-9689	NN	denotes	preclearing
T20508	9668-9677	VB	denotes	Following
T20507	9665-9666	-RRB-	denotes	)
T20506	9659-9665	NNP	denotes	States
T20505	9652-9658	NNP	denotes	United
T20504	9650-9651	-COMMA-	denotes	,
T20503	9644-9650	NNP	denotes	Jersey
T20502	9640-9643	NNP	denotes	New
T20501	9638-9639	-COMMA-	denotes	,
T20500	9628-9638	NNP	denotes	Piscataway
T20499	9626-9627	-COMMA-	denotes	,
T20498	9615-9626	NNP	denotes	Biosciences
T20497	9606-9614	NNP	denotes	Amersham
T20496	9605-9606	-LRB-	denotes	(
T20495	9593-9604	NN	denotes	A-Sepharose
T20494	9585-9592	NN	denotes	protein
T20493	9580-9584	IN	denotes	with
T20492	9569-9579	VB	denotes	precleared
T20491	9565-9568	VB	denotes	was
T20490	9558-9564	NN	denotes	sample
T20489	9548-9557	VB	denotes	remaining
T20488	9544-9547	DT	denotes	the
T20487	9540-9543	CC	denotes	and
T20486	9538-9539	-COMMA-	denotes	,
T20485	9531-9538	NN	denotes	control
T20484	9525-9530	NN	denotes	input
T20483	9521-9524	IN	denotes	for
T20482	9515-9520	RB	denotes	aside
T20481	9511-9514	VB	denotes	set
T20480	9507-9510	VB	denotes	was
T20479	9500-9506	NN	denotes	sample
T20478	9496-9499	DT	denotes	the
T20477	9493-9495	IN	denotes	of
T20476	9483-9492	NN	denotes	One-tenth
T20475	9479-9481	NN	denotes	bp
T20474	9475-9478	CD	denotes	600
T20473	9471-9474	CC	denotes	and
T20472	9467-9470	CD	denotes	200
T20471	9464-9466	TO	denotes	to
T20470	9454-9463	VB	denotes	sonicated
T20469	9449-9453	VB	denotes	were
T20468	9439-9448	NN	denotes	complexes
T20467	9427-9438	JJ	denotes	DNA-protein
T20466	9422-9425	NN	denotes	min
T20465	9419-9421	CD	denotes	10
T20464	9415-9418	IN	denotes	for
T20463	9411-9414	NN	denotes	ice
T20462	9408-9410	IN	denotes	on
T20461	9398-9407	VB	denotes	incubated
T20460	9394-9397	CC	denotes	and
T20459	9392-9393	-COMMA-	denotes	,
T20458	9382-9392	NN	denotes	inhibitors
T20457	9373-9381	NN	denotes	protease
T20456	9362-9372	VB	denotes	containing
T20455	9355-9361	NN	denotes	buffer
T20454	9349-9354	NN	denotes	lysis
T20453	9345-9348	NN	denotes	SDS
T20452	9343-9344	DT	denotes	a
T20451	9340-9342	IN	denotes	in
T20450	9328-9339	VB	denotes	resuspended
T20449	9326-9327	-COMMA-	denotes	,
T20448	9324-9326	NN	denotes	°C
T20447	9322-9323	CD	denotes	4
T20446	9319-9321	IN	denotes	at
T20445	9304-9318	NN	denotes	centrifugation
T20444	9301-9303	IN	denotes	by
T20443	9291-9300	VB	denotes	collected
T20442	9289-9290	-COMMA-	denotes	,
T20441	9279-9289	NN	denotes	inhibitors
T20440	9270-9278	NN	denotes	protease
T20439	9259-9269	VB	denotes	containing
T20438	9254-9258	NN	denotes	EDTA
T20437	9252-9253	NN	denotes	%
T20436	9249-9252	CD	denotes	0.1
T20435	9244-9248	IN	denotes	with
T20434	9235-9243	NN	denotes	solution
T20433	9230-9234	NN	denotes	salt
T20432	9221-9229	JJ	denotes	balanced
T20431	9219-9220	POS	denotes	'
T20430	9214-9219	NNP	denotes	Hanks
T20429	9211-9213	CD	denotes	1×
T20428	9202-9210	JJ	denotes	ice-cold
T20427	9199-9201	IN	denotes	in
T20426	9192-9198	VB	denotes	rinsed
T20425	9190-9191	-COMMA-	denotes	,
T20424	9188-9190	NN	denotes	°C
T20423	9185-9187	CD	denotes	37
T20422	9182-9184	IN	denotes	at
T20421	9178-9181	NN	denotes	min
T20420	9175-9177	CD	denotes	10
T20419	9171-9174	IN	denotes	for
T20418	9158-9170	NN	denotes	formaldehyde
T20417	9156-9157	NN	denotes	%
T20416	9155-9156	CD	denotes	1
T20415	9150-9154	IN	denotes	with
T20414	9142-9149	VB	denotes	treated
T20413	9137-9141	VB	denotes	were
T20412	9132-9136	NN	denotes	mice
T20411	9129-9131	JJ	denotes	WT
T20410	9120-9128	JJ	denotes	15.5-dpc
T20409	9114-9119	CD	denotes	three
T20408	9109-9113	IN	denotes	from
T20407	9103-9108	NN	denotes	cells
T20406	9097-9102	NN	denotes	liver
T20405	9091-9096	JJ	denotes	fetal
T20404	9088-9090	CC	denotes	or
T20403	9086-9087	-RRB-	denotes	)
T20402	9083-9086	CD	denotes	107
T20401	9081-9082	NN	denotes	×
T20400	9079-9080	CD	denotes	4
T20399	9078-9079	-LRB-	denotes	(
T20398	9072-9077	NN	denotes	cells
T20397	9068-9071	NN	denotes	MEL
T20396	9063-9067	IN	denotes	from
T20395	9057-9062	NN	denotes	Cells
T20394	9055-9056	-COLON-	denotes	:
T20393	9050-9055	NN	denotes	brief
T20392	9047-9049	IN	denotes	in
T20391	9045-9046	-COMMA-	denotes	,
T20390	9044-9045	-RRB-	denotes	]
T20389	9042-9044	CD	denotes	53
T20388	9041-9042	-LRB-	denotes	[
T20387	9033-9040	NNP	denotes	Nouzova
T20386	9030-9032	IN	denotes	by
T20385	9020-9029	VB	denotes	described
T20384	9017-9019	IN	denotes	As
T20383	9010-9015	NN	denotes	assay
T20382	9005-9009	NN	denotes	ChIP
T19957	9001-9002	-RRB-	denotes	)
T19956	8994-9001	NN	denotes	MHB1650
T19955	8993-8994	-LRB-	denotes	(
T19954	8952-8992	NN	denotes	5′AATGCAGtgccAAGGGTCAGAACATTGTCTGCGAAG3′
T19953	8950-8951	-COLON-	denotes	:
T19952	8949-8950	-RRB-	denotes	)
T19951	8947-8949	NN	denotes	M3
T19950	8946-8947	-LRB-	denotes	(
T19949	8944-8945	CD	denotes	3
T19948	8938-8943	NN	denotes	probe
T19947	8931-8937	JJ	denotes	mutant
T19946	8927-8930	IN	denotes	for
T19945	8925-8926	-COLON-	denotes	;
T19944	8924-8925	-RRB-	denotes	)
T19943	8917-8924	NN	denotes	MHB1648
T19942	8916-8917	-LRB-	denotes	(
T19940	8873-8874	-COLON-	denotes	:
T19939	8872-8873	-RRB-	denotes	)
T19938	8870-8872	NN	denotes	M2
T19937	8869-8870	-LRB-	denotes	(
T19936	8867-8868	CD	denotes	2
T19935	8861-8866	NN	denotes	probe
T19934	8854-8860	JJ	denotes	mutant
T19933	8850-8853	IN	denotes	for
T19932	8848-8849	-COLON-	denotes	;
T19931	8847-8848	-RRB-	denotes	)
T19930	8840-8847	NN	denotes	MHB1644
T19929	8839-8840	-LRB-	denotes	(
T19928	8805-8838	NN	denotes	5′AACAAAGGGTCAGAACATTGTCTGCGAAG3′
T19927	8803-8804	-COLON-	denotes	:
T19926	8802-8803	-RRB-	denotes	)
T19925	8800-8802	NN	denotes	M1
T19924	8799-8800	-LRB-	denotes	(
T19923	8797-8798	CD	denotes	1
T19922	8791-8796	NN	denotes	probe
T19921	8784-8790	JJ	denotes	mutant
T19920	8780-8783	IN	denotes	for
T19919	8778-8779	-COLON-	denotes	;
T19918	8777-8778	-RRB-	denotes	)
T19917	8770-8777	NN	denotes	MHB1556
T19916	8769-8770	-LRB-	denotes	(
T19915	8728-8768	NN	denotes	5′AATGCAGAACAAAGGGTCAGAACATTGTCTGCGAAG3′
T19914	8726-8727	-COLON-	denotes	:
T19913	8721-8726	NN	denotes	probe
T19912	8718-8720	NN	denotes	WT
T19911	8712-8717	JJ	denotes	36-bp
T19910	8708-8711	DT	denotes	the
T19909	8704-8707	IN	denotes	For
T19908	8702-8703	-COLON-	denotes	:
T19907	8701-8702	-RRB-	denotes	)
T19906	8695-8701	VB	denotes	listed
T19905	8691-8694	VB	denotes	are
T19904	8684-8690	NN	denotes	oligos
T19903	8676-8683	JJ	denotes	forward
T19902	8671-8675	RB	denotes	only
T19901	8670-8671	-LRB-	denotes	(
T19900	8662-8669	NN	denotes	follows
T19899	8659-8661	IN	denotes	as
T19898	8655-8658	VB	denotes	are
T19897	8638-8654	NN	denotes	oligonucleotides
T19896	8634-8637	DT	denotes	the
T19895	8631-8633	IN	denotes	of
T19894	8621-8630	NN	denotes	sequences
T19893	8617-8620	NN	denotes	DNA
T19892	8613-8616	DT	denotes	The
T19891	8610-8611	-RRB-	denotes	)
T19890	8605-8610	JJ	denotes	above
T19889	8602-8604	IN	denotes	as
T19888	8592-8601	VB	denotes	described
T19887	8591-8592	-LRB-	denotes	(
T19886	8580-8590	NN	denotes	antibodies
T19885	8575-8579	NN	denotes	Sox6
T19884	8571-8574	CC	denotes	and
T19883	8569-8570	-COMMA-	denotes	,
T19882	8567-8569	NN	denotes	HA
T19881	8565-8566	-COMMA-	denotes	,
T19880	8560-8565	NN	denotes	c-Myc
T19879	8551-8559	VB	denotes	included
T19878	8542-8550	NN	denotes	analyses
T19877	8531-8541	NN	denotes	supershift
T19876	8527-8530	IN	denotes	for
T19874	8511-8521	NN	denotes	Antibodies
T19873	8494-8509	NN	denotes	autoradiography
T19872	8491-8493	TO	denotes	to
T19871	8485-8490	RB	denotes	prior
T19870	8479-8484	VB	denotes	dried
T19869	8474-8478	VB	denotes	were
T19868	8469-8473	NN	denotes	gels
T19867	8465-8468	DT	denotes	the
T19866	8461-8464	CC	denotes	and
T19865	8459-8460	-COMMA-	denotes	,
T19864	8448-8459	NN	denotes	temperature
T19863	8443-8447	NN	denotes	room
T19862	8440-8442	IN	denotes	at
T19861	8438-8439	NN	denotes	h
T19860	8434-8437	CD	denotes	4–8
T19859	8430-8433	IN	denotes	for
T19858	8425-8429	NN	denotes	mAmp
T19857	8422-8424	CD	denotes	19
T19856	8413-8421	JJ	denotes	constant
T19855	8411-8412	DT	denotes	a
T19854	8408-8410	IN	denotes	at
T19853	8398-8407	VB	denotes	performed
T19852	8394-8397	VB	denotes	was
T19851	8378-8393	NN	denotes	Electrophoresis
T19850	8373-8376	NN	denotes	gel
T19849	8358-8372	NN	denotes	polyacrylamide
T19848	8356-8357	-RRB-	denotes	)
T19847	8353-8356	NN	denotes	w/v
T19846	8352-8353	-LRB-	denotes	(
T19845	8350-8351	NN	denotes	%
T19844	8349-8350	CD	denotes	6
T19843	8346-8348	CC	denotes	or
T19842	8344-8345	NN	denotes	%
T19841	8343-8344	CD	denotes	4
T19840	8341-8342	DT	denotes	a
T19839	8338-8340	IN	denotes	on
T19838	8331-8337	VB	denotes	loaded
T19837	8327-8330	CC	denotes	and
T19836	8315-8326	NN	denotes	temperature
T19835	8310-8314	NN	denotes	room
T19834	8307-8309	IN	denotes	at
T19833	8303-8306	NN	denotes	min
T19832	8300-8302	CD	denotes	60
T19831	8297-8299	CC	denotes	or
T19830	8293-8296	NN	denotes	min
T19829	8290-8292	CD	denotes	30
T19828	8286-8289	IN	denotes	for
T19827	8276-8285	VB	denotes	incubated
T19826	8271-8275	VB	denotes	were
T19825	8263-8270	NN	denotes	samples
T19824	8259-8262	DT	denotes	the
T19823	8257-8258	-COMMA-	denotes	,
T19822	8252-8257	NN	denotes	probe
T19821	8239-8251	VB	denotes	radiolabeled
T19820	8235-8238	DT	denotes	the
T19819	8232-8234	IN	denotes	of
T19818	8223-8231	NN	denotes	addition
T19817	8213-8222	VB	denotes	Following
T19816	8206-8211	NN	denotes	probe
T19815	8193-8205	VB	denotes	radiolabeled
T19814	8190-8192	IN	denotes	of
T19813	8181-8189	NN	denotes	addition
T19812	8178-8180	TO	denotes	to
T19811	8172-8177	JJ	denotes	prior
T19810	8168-8171	NN	denotes	min
T19809	8165-8167	CD	denotes	60
T19808	8162-8164	TO	denotes	to
T19807	8158-8161	NN	denotes	min
T19806	8155-8157	CD	denotes	30
T19805	8149-8154	VB	denotes	added
T19804	8145-8148	VB	denotes	was
T19803	8143-8144	-RRB-	denotes	)
T19802	8141-8143	NN	denotes	μl
T19801	8139-8140	CD	denotes	3
T19800	8138-8139	-LRB-	denotes	(
T19799	8129-8137	NN	denotes	antibody
T19798	8126-8128	CC	denotes	or
T19797	8124-8125	-RRB-	denotes	)
T19796	8118-8124	NN	denotes	excess
T19795	8112-8117	JJ	denotes	molar
T19794	8103-8111	JJ	denotes	200-fold
T19793	8102-8103	-LRB-	denotes	(
T19792	8091-8101	NN	denotes	competitor
T19791	8075-8090	NN	denotes	oligonucleotide
T19790	8064-8074	JJ	denotes	unlabelled
T19789	8054-8063	VB	denotes	indicated
T19788	8050-8053	DT	denotes	the
T19787	8048-8049	-COMMA-	denotes	,
T19786	8042-8048	NN	denotes	assays
T19785	8031-8041	NN	denotes	supershift
T19784	8028-8030	CC	denotes	or
T19783	8016-8027	NN	denotes	competition
T19782	8012-8015	IN	denotes	For
T19781	8005-8010	NN	denotes	MgCl2
T19780	8002-8004	NN	denotes	mM
T19779	7998-8001	CD	denotes	0.6
T19778	7996-7997	-COMMA-	denotes	,
T19777	7993-7996	NN	denotes	DTT
T19776	7990-7992	NN	denotes	mM
T19775	7985-7989	CD	denotes	0.25
T19705	7680-7681	-RRB-	denotes	]
T19704	7675-7680	NN	denotes	γ-32P
T19703	7674-7675	-LRB-	denotes	[
T19702	7669-7673	IN	denotes	with
T19701	7657-7668	VB	denotes	end-labeled
T19700	7653-7656	CC	denotes	and
T19699	7644-7652	VB	denotes	annealed
T19698	7639-7643	VB	denotes	were
T19697	7622-7638	NN	denotes	oligonucleotides
T19696	7608-7621	JJ	denotes	complementary
T19695	7592-7607	JJ	denotes	Single-stranded
T19694	7586-7590	NN	denotes	EMSA
T19503	7582-7583	-RRB-	denotes	)
T19502	7576-7582	NNP	denotes	States
T19501	7569-7575	NNP	denotes	United
T19500	7567-7568	-COMMA-	denotes	,
T19499	7563-7567	NNP	denotes	York
T19498	7559-7562	NNP	denotes	New
T19497	7557-7558	-COMMA-	denotes	,
T19496	7551-7557	NNP	denotes	Placid
T19495	7546-7550	NNP	denotes	Lake
T19494	7545-7546	-LRB-	denotes	(
T19493	7531-7544	NNP	denotes	Biotechnology
T19492	7523-7530	NNP	denotes	Upstate
T19491	7518-7522	IN	denotes	from
T19490	7509-7517	VB	denotes	obtained
T19240	7127-7134	NN	denotes	control
T19239	7118-7126	JJ	denotes	negative
T19238	7116-7117	DT	denotes	a
T19237	7113-7115	IN	denotes	as
T19236	7102-7112	VB	denotes	translated
T19235	7097-7101	RB	denotes	also
T19234	7093-7096	VB	denotes	was
T19233	7084-7092	NN	denotes	sequence
T19232	7077-7083	NN	denotes	coding
T19231	7072-7076	NN	denotes	Sox6
T19230	7068-7071	DT	denotes	the
T19229	7060-7067	IN	denotes	without
T19228	7053-7059	NN	denotes	vector
T19227	7051-7052	DT	denotes	A
T19226	7048-7049	-RRB-	denotes	)
T19225	7041-7048	NNP	denotes	Promega
T19224	7039-7040	-COMMA-	denotes	,
T19223	7032-7039	NNP	denotes	Systems
T19222	7006-7031	NNP	denotes	Transcription/Translation
T19221	6998-7005	NNP	denotes	Coupled
T19220	6992-6997	NNP	denotes	Quick
T19219	6987-6991	NNP	denotes	TNT®
T19218	6986-6987	-LRB-	denotes	(
T19217	6979-6985	NN	denotes	system
T19216	6965-6978	JJ	denotes	translational
T19215	6959-6964	FW	denotes	vitro
T19214	6956-6958	FW	denotes	in
T19213	6950-6955	VB	denotes	based
T19212	6943-6949	NN	denotes	lysate
T19211	6930-6942	NN	denotes	reticulocyte
T19210	6928-6929	DT	denotes	a
T19209	6925-6927	IN	denotes	in
T19208	6915-6924	VB	denotes	performed
T19207	6911-6914	VB	denotes	was
T19206	6899-6910	NN	denotes	translation
T19205	6895-6898	DT	denotes	The
T19204	6892-6893	-RRB-	denotes	]
T19203	6890-6892	CD	denotes	15
T19202	6889-6890	-LRB-	denotes	[
T19201	6882-6888	IN	denotes	before
T19199	6868-6871	VB	denotes	was
T19198	6866-6867	-COMMA-	denotes	,
T19197	6864-6866	NN	denotes	HA
T19196	6860-6863	CC	denotes	and
T19195	6854-6859	NN	denotes	c-Myc
T19194	6849-6853	IN	denotes	with
T19193	6842-6848	VB	denotes	tagged
T19192	6840-6841	-COMMA-	denotes	,
T19191	6834-6840	NN	denotes	vector
T19190	6823-6833	NN	denotes	expression
T19189	6811-6822	NN	denotes	translation
T19188	6805-6810	FW	denotes	vitro
T19187	6802-6804	FW	denotes	in
T19186	6797-6801	NN	denotes	Sox6
T19185	6793-6796	DT	denotes	The
T19184	6790-6791	-RRB-	denotes	)
T19183	6784-6790	NNP	denotes	States
T19182	6777-6783	NNP	denotes	United
T19181	6775-6776	-COMMA-	denotes	,
T19180	6765-6775	NNP	denotes	California
T19179	6763-6764	-COMMA-	denotes	,
T19178	6755-6763	NNP	denotes	Carlsbad
T19177	6753-6754	-COMMA-	denotes	,
T19176	6748-6753	NNP	denotes	Motif
T19175	6741-6747	JJ	denotes	Active
T19174	6740-6741	-LRB-	denotes	(
T19173	6736-6739	NN	denotes	kit
T19172	6734-6735	DT	denotes	a
T19171	6728-6733	VB	denotes	using
T19170	6726-6727	-RRB-	denotes	)
T19169	6723-6726	CD	denotes	107
T19168	6721-6722	CC	denotes	×
T19167	6719-6720	CD	denotes	2
T19166	6718-6719	-LRB-	denotes	(
T19165	6712-6717	NN	denotes	cells
T19164	6708-6711	NN	denotes	MEL
T19163	6703-6707	IN	denotes	from
T19162	6694-6702	VB	denotes	prepared
T19161	6689-6693	VB	denotes	were
T19160	6680-6688	NN	denotes	extracts
T19159	6672-6679	JJ	denotes	Nuclear
T19774	7983-7984	-COMMA-	denotes	,
T19773	7979-7983	NN	denotes	EDTA
T19772	7976-7978	NN	denotes	mM
T19771	7972-7975	CD	denotes	0.1
T19770	7970-7971	-COMMA-	denotes	,
T19769	7969-7970	-RRB-	denotes	)
T19768	7968-7969	CD	denotes	7
T19767	7965-7967	NN	denotes	pH
T19766	7964-7965	-LRB-	denotes	(
T19765	7958-7963	NN	denotes	HEPES
T19764	7955-7957	NN	denotes	mM
T19763	7952-7954	CD	denotes	10
T19762	7950-7951	-COMMA-	denotes	,
T19761	7949-7950	-RRB-	denotes	)
T19760	7944-7949	NN	denotes	dG-dC
T19759	7943-7944	-LRB-	denotes	(
T19758	7938-7942	NN	denotes	poly
T19757	7935-7937	CC	denotes	or
T19756	7933-7934	-RRB-	denotes	)
T19755	7928-7933	NN	denotes	dI-dC
T19754	7927-7928	-LRB-	denotes	(
T19753	7922-7926	NN	denotes	poly
T19752	7916-7921	NN	denotes	ng/μl
T19751	7913-7915	CD	denotes	50
T19750	7911-7912	-COMMA-	denotes	,
T19749	7908-7911	NN	denotes	BSA
T19748	7902-7907	NN	denotes	ng/μl
T19747	7898-7901	CD	denotes	200
T19746	7896-7897	-COMMA-	denotes	,
T19745	7888-7896	NN	denotes	glycerol
T19744	7886-7887	NN	denotes	%
T19743	7884-7886	CD	denotes	10
T19742	7882-7883	-COMMA-	denotes	,
T19741	7878-7882	NN	denotes	NaCl
T19740	7875-7877	NN	denotes	mM
T19739	7871-7874	CD	denotes	100
T19738	7869-7870	-COLON-	denotes	:
T19737	7863-7869	NN	denotes	buffer
T19736	7855-7862	NN	denotes	binding
T19735	7852-7854	IN	denotes	in
T19734	7845-7851	NN	denotes	lysate
T19733	7832-7844	NN	denotes	reticulocyte
T19732	7828-7831	DT	denotes	the
T19731	7823-7827	IN	denotes	with
T19730	7817-7822	IN	denotes	along
T19729	7812-7816	NN	denotes	Sox6
T19728	7795-7811	JJ	denotes	vitro-translated
T19727	7792-7794	FW	denotes	in
T19726	7789-7791	IN	denotes	of
T19725	7786-7788	NN	denotes	μl
T19724	7784-7785	CD	denotes	3
T19723	7781-7783	CC	denotes	or
T19722	7775-7780	NN	denotes	cells
T19721	7771-7774	NN	denotes	MEL
T19720	7766-7770	IN	denotes	from
T19719	7757-7765	NN	denotes	proteins
T19718	7749-7756	JJ	denotes	nuclear
T19717	7746-7748	IN	denotes	of
T19716	7743-7745	NN	denotes	μg
T19715	7741-7742	CD	denotes	5
T19714	7736-7740	IN	denotes	with
T19713	7726-7735	VB	denotes	performed
T19712	7722-7725	VB	denotes	was
T19711	7717-7721	NN	denotes	EMSA
T19710	7709-7715	NN	denotes	kinase
T19709	7694-7708	NN	denotes	polynucleotide
T19708	7691-7693	NN	denotes	T4
T19707	7686-7690	IN	denotes	with
T19706	7682-7685	NN	denotes	ATP
T19158	6666-6670	NN	denotes	Sox6
T19157	6663-6665	IN	denotes	of
T19156	6651-6662	NN	denotes	translation
T19155	6645-6650	FW	denotes	vitro
T19154	6642-6644	FW	denotes	in
T19153	6638-6641	CC	denotes	and
T19152	6630-6637	NN	denotes	extract
T19151	6622-6629	NN	denotes	protein
T19150	6614-6621	JJ	denotes	Nuclear
T19463	7330-7343	NNP	denotes	Biotechnology
T19462	7325-7329	NNP	denotes	Cruz
T19461	7319-7324	NNP	denotes	Santa
T19460	7317-7318	-COMMA-	denotes	,
T19459	7316-7317	NNP	denotes	X
T19458	7307-7315	NN	denotes	sc-17332
T19457	7303-7306	NN	denotes	No.
T19456	7295-7302	NNP	denotes	Catalog
T19455	7294-7295	-LRB-	denotes	(
T19454	7285-7293	VB	denotes	obtained
T19453	7272-7284	RB	denotes	commercially
T19452	7269-7271	CC	denotes	or
T19451	7267-7268	-RRB-	denotes	)
T19450	7266-7267	-RRB-	denotes	]
T19449	7265-7266	CD	denotes	7
T19448	7264-7265	-LRB-	denotes	[
T19447	7257-7263	NNP	denotes	France
T19446	7255-7256	-COMMA-	denotes	,
T19445	7248-7255	NNP	denotes	Pasteur
T19444	7242-7247	NNP	denotes	Louis
T19443	7231-7241	NNP	denotes	Université
T19442	7230-7231	-LRB-	denotes	(
T19441	7224-7229	NNP	denotes	Lalli
T19440	7219-7223	NNP	denotes	Enzo
T19439	7215-7218	NNP	denotes	Dr.
T19438	7212-7214	IN	denotes	by
T19437	7203-7211	VB	denotes	provided
T19436	7196-7202	RB	denotes	kindly
T19435	7189-7195	CC	denotes	either
T19434	7184-7188	VB	denotes	were
T19433	7178-7183	NN	denotes	study
T19432	7173-7177	DT	denotes	this
T19431	7170-7172	IN	denotes	in
T19430	7165-7169	VB	denotes	used
T19429	7154-7164	NN	denotes	antibodies
T19428	7149-7153	NN	denotes	Sox6
T19427	7137-7147	NN	denotes	Antibodies
T18887	6589-6599	NN	denotes	ransfectio
T18886	6577-6589	NN	denotes	ontrol for t
T18885	6574-6577	IN	denotes	a c
T18884	6567-6574	NN	denotes	sed as
T18883	6566-6567	DT	denotes	u
T18882	6564-6566	IN	denotes	s
T18881	6560-6564	VB	denotes	) wa
T18880	6557-6560	VB	denotes	ega
T18879	6556-6557	-RRB-	denotes	m
T18878	6549-6556	NN	denotes	ng (Pro
T18877	6548-6549	-LRB-	denotes	5
T18876	6544-6548	NN	denotes	MV 1
T18875	6537-6544	NN	denotes	. pRL-C
T18874	6536-6537	-RRB-	denotes	)
T18873	6534-6536	NN	denotes	ng
T18872	6529-6533	CD	denotes	1000
T18871	6525-6528	CC	denotes	and
T18870	6523-6524	-COMMA-	denotes	,
T18869	6521-6523	NN	denotes	ng
T18868	6517-6520	CD	denotes	500
T18867	6515-6516	-COMMA-	denotes	,
T18866	6513-6515	NN	denotes	ng
T18865	6509-6512	CD	denotes	200
T18864	6504-6508	VB	denotes	used
T18863	6501-6503	PRP	denotes	we
T18862	6499-6500	-COMMA-	denotes	,
T18861	6493-6499	NN	denotes	effect
T18860	6486-6492	NN	denotes	dosage
T18859	6483-6485	IN	denotes	of
T18858	6476-6482	NN	denotes	assays
T18857	6473-6475	IN	denotes	In
T18856	6470-6471	-RRB-	denotes	)
T18855	6468-6470	NN	denotes	ng
T18854	6463-6467	CD	denotes	1000
T18853	6462-6463	-LRB-	denotes	(
T18852	6455-6461	NN	denotes	vector
T18851	6440-6454	NN	denotes	overexpression
T18850	6435-6439	NN	denotes	Sox6
T18849	6432-6434	CC	denotes	or
T18848	6425-6431	NN	denotes	vector
T18847	6419-6424	JJ	denotes	empty
T18846	6412-6418	CC	denotes	either
T18845	6407-6411	IN	denotes	with
T18844	6401-6406	IN	denotes	along
T18843	6399-6400	-RRB-	denotes	)
T18842	6397-6399	NN	denotes	ng
T18841	6393-6396	CD	denotes	500
T18840	6392-6393	-LRB-	denotes	(
T18839	6381-6391	NN	denotes	constructs
T18838	6372-6380	NN	denotes	reporter
T18837	6363-6371	NN	denotes	promoter
T18836	6360-6362	JJ	denotes	ɛy
T18785	6098-6099	CD	denotes	2
T18784	6097-6098	-LRB-	denotes	(
T18783	6085-6096	NN	denotes	L-glutamine
T18782	6081-6084	CC	denotes	and
T18781	6079-6080	-COMMA-	denotes	,
T18780	6078-6079	-RRB-	denotes	)
T18779	6073-6078	NN	denotes	μg/ml
T18778	6069-6072	CD	denotes	100
T18777	6056-6068	NN	denotes	streptomycin
T18776	6054-6055	-COMMA-	denotes	,
T18775	6053-6054	-RRB-	denotes	)
T18774	6045-6053	NN	denotes	units/ml
T18773	6041-6044	CD	denotes	100
T18772	6040-6041	-LRB-	denotes	(
T18771	6029-6039	NN	denotes	penicillin
T18770	6027-6028	-COMMA-	denotes	,
T18769	6026-6027	-RRB-	denotes	)
T18768	6020-6026	NNP	denotes	States
T18767	6013-6019	NNP	denotes	United
T18766	6011-6012	-COMMA-	denotes	,
T18765	6001-6011	NNP	denotes	California
T18764	5999-6000	-COMMA-	denotes	,
T18763	5991-5999	NNP	denotes	Carlsbad
T18762	5989-5990	-COMMA-	denotes	,
T18761	5980-5989	NN	denotes	Ivitrogen
T18760	5979-5980	-LRB-	denotes	(
T18759	5973-5978	NN	denotes	serum
T18758	5968-5972	NN	denotes	calf
T18757	5962-5967	JJ	denotes	fetal
T18756	5960-5961	NN	denotes	%
T18755	5958-5960	CD	denotes	10
T18754	5941-5957	JJ	denotes	heat-inactivated
T18753	5936-5940	IN	denotes	with
T18752	5923-5935	VB	denotes	supplemented
T18751	5911-5922	NN	denotes	L-glutamine
T18750	5908-5910	NN	denotes	mM
T18749	5906-5907	CD	denotes	2
T18748	5901-5905	IN	denotes	with
T18747	5897-5900	NN	denotes	F12
T18746	5894-5896	POS	denotes	's
T18745	5891-5894	NNP	denotes	Ham
T18744	5888-5890	IN	denotes	in
T18743	5879-5887	VB	denotes	cultured
T18742	5874-5878	VB	denotes	were
T18741	5872-5873	-RRB-	denotes	)
T18740	5866-5872	NNP	denotes	States
T18739	5859-5865	NNP	denotes	United
T18738	5857-5858	-COMMA-	denotes	,
T18737	5851-5857	NNP	denotes	Jersey
T18736	5847-5850	NNP	denotes	New
T18735	5845-5846	-COMMA-	denotes	,
T18734	5839-5845	NNP	denotes	Camden
T18733	5837-5838	-COMMA-	denotes	,
T18732	5825-5837	NNP	denotes	Repositories
T18731	5820-5824	NNP	denotes	Cell
T18730	5812-5819	NNP	denotes	Coriell
T18729	5811-5812	-LRB-	denotes	(
T18728	5805-5810	NN	denotes	cells
T18727	5799-5804	NN	denotes	GM979
T18726	5785-5797	NN	denotes	transfection
T18725	5781-5784	CC	denotes	and
T18724	5773-5780	NN	denotes	culture
T18723	5768-5772	NN	denotes	Cell
T19489	7505-7508	VB	denotes	was
T19488	7496-7504	NN	denotes	antibody
T19487	7492-7495	NN	denotes	IgG
T19486	7485-7491	NN	denotes	rabbit
T19485	7478-7484	NNP	denotes	Normal
T19484	7466-7477	NNP	denotes	Invitrogen.
T19483	7461-7465	IN	denotes	from
T19482	7451-7460	VB	denotes	purchased
T19481	7447-7450	VB	denotes	was
T19480	7438-7446	NN	denotes	antibody
T19479	7432-7437	NN	denotes	c-Myc
T19478	7423-7431	JJ	denotes	results.
T19477	7415-7422	JJ	denotes	similar
T19476	7405-7414	VB	denotes	generated
T19475	7394-7404	NN	denotes	antibodies
T19474	7389-7393	NN	denotes	Sox6
T19473	7385-7388	DT	denotes	All
T19472	7382-7383	-RRB-	denotes	)
T19471	7376-7382	NNP	denotes	States
T19470	7369-7375	NNP	denotes	United
T19469	7367-7368	-COMMA-	denotes	,
T19468	7357-7367	NNP	denotes	California
T19467	7355-7356	-COMMA-	denotes	,
T19466	7351-7355	NNP	denotes	Cruz
T19465	7345-7350	NNP	denotes	Santa
T19464	7343-7344	-COMMA-	denotes	,
T18301	5207-5216	JJ	denotes	available
T18300	5203-5206	VB	denotes	are
T18299	5196-5202	NN	denotes	images
T18298	5187-5195	JJ	denotes	Original
T18297	5181-5185	CD	denotes	4300
T18296	5173-5180	NNP	denotes	Coolpix
T18295	5167-5172	NNP	denotes	Nikon
T18294	5165-5166	DT	denotes	a
T18293	5161-5164	VB	denotes	was
T18292	5154-5160	NN	denotes	camera
T18835	6355-6359	IN	denotes	with
T18834	6343-6354	VB	denotes	transfected
T18833	6338-6342	VB	denotes	were
T18832	6332-6337	NN	denotes	Cells
T18831	6329-6330	-RRB-	denotes	)
T18830	6323-6329	NNP	denotes	States
T18829	6316-6322	NNP	denotes	United
T18828	6314-6315	-COMMA-	denotes	,
T18827	6307-6314	NNP	denotes	Indiana
T18826	6305-6306	-COMMA-	denotes	,
T18825	6293-6305	NNP	denotes	Indianapolis
T18824	6291-6292	-COMMA-	denotes	,
T18823	6286-6291	NNP	denotes	Roche
T18822	6285-6286	-LRB-	denotes	(
T18821	6277-6284	NN	denotes	FuGENE6
T18820	6274-6276	IN	denotes	by
T18819	6265-6273	NN	denotes	plasmids
T18818	6260-6264	IN	denotes	with
T18817	6248-6259	VB	denotes	transfected
T18816	6243-6247	VB	denotes	were
T18815	6236-6242	NN	denotes	growth
T18814	6233-6235	IN	denotes	of
T18813	6227-6232	NN	denotes	phase
T18812	6223-6226	NN	denotes	log
T18811	6220-6222	IN	denotes	in
T18810	6218-6219	-RRB-	denotes	)
T18809	6215-6218	CD	denotes	105
T18808	6213-6214	NN	denotes	×
T18807	6211-6212	CD	denotes	4
T18806	6210-6211	-LRB-	denotes	(
T18805	6204-6209	NN	denotes	cells
T18804	6198-6203	NN	denotes	GM979
T18803	6195-6196	-RRB-	denotes	)
T18802	6190-6195	NN	denotes	serum
T18801	6186-6189	DT	denotes	the
T18800	6173-6185	VB	denotes	inactivating
T18799	6168-6172	NN	denotes	heat
T18798	6160-6167	IN	denotes	without
T18797	6159-6160	-LRB-	denotes	(
T18796	6153-6158	JJ	denotes	above
T18795	6150-6152	IN	denotes	as
T18794	6137-6149	VB	denotes	supplemented
T18793	6132-6136	NN	denotes	DMEM
T18792	6129-6131	IN	denotes	in
T18791	6120-6128	VB	denotes	cultured
T18790	6115-6119	VB	denotes	were
T18789	6109-6114	NN	denotes	cells
T18788	6105-6108	NN	denotes	MEL
T18787	6102-6103	-RRB-	denotes	)
T18786	6100-6102	NN	denotes	mM
T18291	5150-5153	DT	denotes	The
T18290	5147-5148	-RRB-	denotes	)
T18289	5138-5147	NN	denotes	objective
T18288	5133-5137	CD	denotes	100×
T18287	5132-5133	-LRB-	denotes	(
T18286	5126-5131	NNP	denotes	Nikon
T18285	5117-5125	CD	denotes	160/0.17
T18284	5113-5116	NN	denotes	oil
T18283	5104-5112	CD	denotes	100/1.25
T18282	5099-5103	NNP	denotes	Plan
T18281	5097-5098	NN	denotes	E
T18280	5095-5096	-COMMA-	denotes	,
T18279	5094-5095	-RRB-	denotes	)
T18278	5085-5094	NN	denotes	objective
T18277	5081-5084	CD	denotes	40×
T18276	5080-5081	-LRB-	denotes	(
T18275	5074-5079	NNP	denotes	Nikon
T18274	5065-5073	CD	denotes	160/0.17
T18273	5057-5064	CD	denotes	40/0.65
T18272	5052-5056	NNP	denotes	Plan
T18271	5050-5051	NN	denotes	E
T18270	5045-5049	VB	denotes	were
T18269	5040-5044	VB	denotes	used
T18268	5029-5039	NN	denotes	Objectives
T18267	5017-5027	NN	denotes	microscope
T18266	5006-5016	NN	denotes	Labophot-2
T18265	5000-5005	NNP	denotes	Nikon
T18264	4995-4999	IN	denotes	with
T18263	4986-4994	VB	denotes	obtained
T18262	4981-4985	VB	denotes	were
T18261	4974-4980	NN	denotes	Images
T18260	4966-4972	NN	denotes	manner
T18259	4958-4965	JJ	denotes	similar
T18258	4956-4957	DT	denotes	a
T18257	4953-4955	IN	denotes	in
T18256	4944-4952	VB	denotes	prepared
T18255	4939-4943	VB	denotes	were
T18254	4937-4938	-RRB-	denotes	)
T18253	4934-4937	NN	denotes	dpc
T18252	4929-4933	CD	denotes	18.5
T18251	4925-4928	CC	denotes	and
T18250	4921-4924	NN	denotes	dpc
T18249	4916-4920	CD	denotes	14.5
T18248	4913-4915	IN	denotes	at
T18247	4912-4913	-LRB-	denotes	(
T18246	4904-4911	NN	denotes	samples
T18245	4898-4903	NN	denotes	Liver
T18244	4891-4896	NN	denotes	eosin
T18243	4887-4890	CC	denotes	and
T18242	4875-4886	NN	denotes	hematoxylin
T18241	4870-4874	IN	denotes	with
T18240	4862-4869	VB	denotes	stained
T18239	4858-4861	CC	denotes	and
T18238	4856-4857	-COMMA-	denotes	,
T18237	4854-4856	NN	denotes	μm
T18236	4852-4853	CD	denotes	5
T18235	4849-4851	IN	denotes	at
T18234	4839-4848	VB	denotes	sectioned
T18233	4837-4838	-COMMA-	denotes	,
T18232	4820-4837	JJ	denotes	paraffin-embedded
T18231	4818-4819	-COMMA-	denotes	,
T18230	4810-4818	NN	denotes	formalin
T18229	4808-4809	NN	denotes	%
T18228	4806-4808	CD	denotes	10
T18227	4803-4805	IN	denotes	in
T18226	4797-4802	VB	denotes	fixed
T18225	4792-4796	VB	denotes	were
T18224	4787-4791	NN	denotes	mice
T18223	4778-4786	NN	denotes	day–10.5
T18222	4768-4777	JJ	denotes	postnatal
T18221	4764-4767	CC	denotes	and
T18220	4762-4763	-COMMA-	denotes	,
T18219	4755-4762	NN	denotes	embryos
T18218	4748-4754	JJ	denotes	mutant
T18217	4744-4747	CC	denotes	and
T18216	4741-4743	NN	denotes	WT
T18215	4732-4740	JJ	denotes	14.5-dpc
T18214	4730-4731	-COMMA-	denotes	,
T18213	4722-4730	NN	denotes	analysis
T18212	4716-4721	NN	denotes	mount
T18211	4710-4715	JJ	denotes	whole
T18210	4706-4709	IN	denotes	For
T18209	4701-4704	NNP	denotes	DAF
T18208	4698-4700	IN	denotes	by
T18207	4693-4697	VB	denotes	read
T18206	4689-4692	CC	denotes	and
T18205	4674-4688	JJ	denotes	Wright-stained
T18204	4669-4673	VB	denotes	were
T18203	4662-4668	NN	denotes	slides
T18202	4658-4661	DT	denotes	The
T18201	4652-4656	NN	denotes	mice
T18200	4649-4651	JJ	denotes	WT
T18199	4645-4648	CC	denotes	and
T18198	4638-4644	JJ	denotes	mutant
T18197	4633-4637	CC	denotes	both
T18196	4628-4632	IN	denotes	from
T18195	4619-4627	VB	denotes	prepared
T18194	4614-4618	VB	denotes	were
T18193	4607-4613	NN	denotes	smears
T18192	4601-4606	NN	denotes	blood
T18191	4590-4600	JJ	denotes	peripheral
T17944	4527-4536	JJ	denotes	available
T17943	4523-4526	VB	denotes	are
T17942	4516-4522	NN	denotes	images
T17941	4507-4515	JJ	denotes	Original
T17940	4498-4505	NN	denotes	plugins
T17939	4496-4497	-RRB-	denotes	)
T17938	4490-4496	NNP	denotes	States
T17937	4483-4489	NNP	denotes	United
T17936	4481-4482	-COMMA-	denotes	,
T17935	4473-4481	NNP	denotes	Carolina
T17934	4467-4472	NNP	denotes	North
T17933	4465-4466	-COMMA-	denotes	,
T17932	4456-4465	NNP	denotes	Asheville
T17931	4454-4455	-COMMA-	denotes	,
T17930	4446-4454	NNP	denotes	Graphics
T18565	5764-5765	NN	denotes	d
T18564	5762-5763	CD	denotes	6
T18563	5758-5761	IN	denotes	for
T18562	5755-5757	NN	denotes	°C
T18561	5751-5754	CD	denotes	−80
T18560	5748-5750	IN	denotes	at
T18559	5746-5747	-RRB-	denotes	)
T18558	5740-5746	NNP	denotes	States
T18557	5733-5739	NNP	denotes	United
T18556	5731-5732	-COMMA-	denotes	,
T18555	5727-5731	NNP	denotes	York
T18554	5723-5726	NNP	denotes	New
T18553	5721-5722	-COMMA-	denotes	,
T18552	5712-5721	NNP	denotes	Rochester
T18551	5710-5711	-COMMA-	denotes	,
T18550	5705-5710	NNP	denotes	Kodak
T18549	5704-5705	-LRB-	denotes	(
T18548	5699-5703	NN	denotes	film
T18547	5693-5698	NN	denotes	X-ray
T18546	5690-5692	TO	denotes	to
T18545	5681-5689	NN	denotes	exposure
T18544	5678-5680	TO	denotes	to
T18543	5672-5677	JJ	denotes	prior
T18542	5669-5671	NN	denotes	°C
T18541	5666-5668	CD	denotes	60
T18540	5663-5665	IN	denotes	at
T18539	5659-5662	NN	denotes	SDS
T18538	5657-5658	NN	denotes	%
T18537	5656-5657	CD	denotes	1
T18536	5654-5655	-COMMA-	denotes	,
T18535	5651-5654	NNP	denotes	SSC
T18534	5646-5650	CD	denotes	0.2×
T18533	5641-5645	IN	denotes	with
T18532	5634-5640	VB	denotes	washed
T18531	5629-5633	VB	denotes	were
T18530	5623-5628	NN	denotes	Blots
T18529	5613-5621	NN	denotes	solution
T18528	5599-5612	NN	denotes	hybridization
T18527	5595-5598	NN	denotes	SDS
T18526	5593-5594	NN	denotes	%
T18525	5592-5593	CD	denotes	7
T18524	5583-5591	VB	denotes	buffered
T18523	5573-5582	NN	denotes	phosphate
T18522	5570-5572	IN	denotes	in
T18521	5560-5569	VB	denotes	performed
T18520	5556-5559	VB	denotes	was
T18519	5542-5555	NN	denotes	hybridization
T18518	5538-5541	DT	denotes	The
T18517	5535-5536	-RRB-	denotes	)
T18516	5528-5535	NNP	denotes	Kingdom
T18515	5521-5527	NNP	denotes	United
T18514	5519-5520	-COMMA-	denotes	,
T18513	5512-5519	NNP	denotes	England
T18512	5510-5511	-COMMA-	denotes	,
T18511	5495-5510	NNP	denotes	Buckinghamshire
T18510	5493-5494	-COMMA-	denotes	,
T18509	5482-5493	NNP	denotes	Biosciences
T18508	5473-5481	NNP	denotes	Amersham
T18507	5471-5472	-COLON-	denotes	;
T18506	5460-5471	NN	denotes	RediprimeII
T18505	5459-5460	-LRB-	denotes	(
T18504	5450-5458	NN	denotes	labeling
T18503	5443-5449	NN	denotes	primer
T18502	5436-5442	JJ	denotes	random
T18501	5433-5435	IN	denotes	by
T18500	5431-5432	-COMMA-	denotes	,
T18499	5427-5431	NN	denotes	dCTP
T18498	5426-5427	-RRB-	denotes	]
T18497	5421-5426	NN	denotes	α-32P
T18496	5420-5421	-LRB-	denotes	[
T18495	5415-5419	IN	denotes	with
T18494	5407-5414	VB	denotes	labeled
T18493	5403-5406	CC	denotes	and
T18492	5401-5402	-RRB-	denotes	)
T18491	5392-5401	CD	denotes	1353–1927
T18490	5380-5391	NN	denotes	nucleotides
T18489	5379-5380	-LRB-	denotes	(
T18488	5372-5378	NN	denotes	RT-PCR
T18487	5369-5371	IN	denotes	by
T18486	5359-5368	VB	denotes	generated
T18485	5353-5358	NN	denotes	probe
T18484	5348-5352	NN	denotes	Sox6
T18483	5346-5347	DT	denotes	a
T18482	5341-5345	IN	denotes	with
T18481	5330-5340	VB	denotes	hybridized
T18480	5326-5329	VB	denotes	was
T18479	5324-5325	-RRB-	denotes	)
T18478	5318-5324	NNP	denotes	States
T18477	5311-5317	NNP	denotes	United
T18476	5309-5310	-COMMA-	denotes	,
T18475	5301-5309	NNP	denotes	Maryland
T18474	5299-5300	-COMMA-	denotes	,
T18473	5290-5299	NNP	denotes	Rockville
T18472	5288-5289	-COMMA-	denotes	,
T18471	5281-5288	NN	denotes	Seegene
T18470	5280-5281	-LRB-	denotes	(
T18469	5273-5279	NN	denotes	filter
T18468	5268-5272	NN	denotes	blot
T18467	5259-5267	NN	denotes	Northern
T18466	5252-5258	NN	denotes	tissue
T18465	5242-5251	JJ	denotes	embryonic
T18464	5236-5241	NN	denotes	mouse
T18463	5234-5235	DT	denotes	A
T18462	5228-5232	NN	denotes	blot
T18461	5219-5227	NN	denotes	Northern
T17929	4437-4445	NNP	denotes	Reindeer
T17928	4436-4437	-LRB-	denotes	(
T17927	4432-4435	NNP	denotes	Pro
T17926	4426-4431	NNP	denotes	Fovea
T17925	4421-4425	IN	denotes	with
T17924	4412-4420	NN	denotes	software
T17923	4410-4411	-RRB-	denotes	)
T17922	4404-4410	NNP	denotes	States
T17921	4397-4403	NNP	denotes	United
T17920	4395-4396	-COMMA-	denotes	,
T17919	4385-4395	NNP	denotes	California
T17918	4383-4384	-COMMA-	denotes	,
T17917	4379-4383	NNP	denotes	Jose
T17916	4375-4378	NNP	denotes	San
T17915	4373-4374	-COMMA-	denotes	,
T17914	4368-4373	NNP	denotes	Adobe
T17913	4367-4368	-LRB-	denotes	(
T17912	4357-4366	NNP	denotes	Photoshop
T17911	4351-4356	VB	denotes	using
T17910	4342-4350	VB	denotes	combined
T17909	4338-4341	CC	denotes	and
T17908	4336-4337	-COMMA-	denotes	,
T17907	4323-4336	VB	denotes	pseudocolored
T17906	4321-4322	-COMMA-	denotes	,
T17905	4312-4321	VB	denotes	processed
T17904	4307-4311	VB	denotes	were
T17903	4300-4306	NN	denotes	Images
T17902	4297-4298	-RRB-	denotes	)
T17901	4294-4297	CD	denotes	0.5
T17900	4292-4293	SYM	denotes	=
T17899	4289-4291	NN	denotes	NA
T17898	4288-4289	-LRB-	denotes	(
T17897	4284-4287	CD	denotes	10×
T17896	4280-4283	CC	denotes	and
T17895	4278-4279	-RRB-	denotes	)
T17894	4274-4278	CD	denotes	0.04
T17893	4272-4273	SYM	denotes	=
T17892	4269-4271	NN	denotes	NA
T17891	4268-4269	-LRB-	denotes	(
T17890	4265-4267	CD	denotes	1×
T17889	4260-4264	VB	denotes	were
T17888	4255-4259	VB	denotes	used
T17887	4244-4254	NN	denotes	Objectives
T17886	4241-4242	-RRB-	denotes	)
T17885	4235-4241	NNP	denotes	States
T17884	4228-4234	NNP	denotes	United
T17883	4226-4227	-COMMA-	denotes	,
T17882	4218-4226	NNP	denotes	Michigan
T17881	4216-4217	-COMMA-	denotes	,
T17880	4209-4216	NNP	denotes	Heights
T17879	4200-4208	NNP	denotes	Sterling
T17878	4198-4199	-COMMA-	denotes	,
T17877	4187-4198	NNP	denotes	Instruments
T17876	4176-4186	JJ	denotes	Diagnostic
T17875	4175-4176	-LRB-	denotes	(
T17874	4168-4174	NN	denotes	camera
T17873	4160-4167	JJ	denotes	digital
T17872	4150-4159	NN	denotes	RT-Slider
T17871	4145-4149	NN	denotes	SPOT
T17870	4141-4144	CC	denotes	and
T17869	4139-4140	-RRB-	denotes	)
T17868	4133-4139	NNP	denotes	States
T17867	4126-4132	NNP	denotes	United
T17866	4124-4125	-COMMA-	denotes	,
T17865	4120-4124	NNP	denotes	York
T17864	4116-4119	NNP	denotes	New
T17863	4114-4115	-COMMA-	denotes	,
T17862	4106-4114	NNP	denotes	Melville
T17861	4104-4105	-COMMA-	denotes	,
T17860	4099-4104	NNP	denotes	Nikon
T17859	4098-4099	-LRB-	denotes	(
T17858	4087-4097	NN	denotes	microscope
T17857	4078-4086	NNP	denotes	Optiphot
T17856	4072-4077	NNP	denotes	Nikon
T17855	4070-4071	DT	denotes	a
T17854	4065-4069	IN	denotes	with
T17853	4056-4064	VB	denotes	obtained
T17852	4051-4055	VB	denotes	were
T17851	4044-4050	NN	denotes	images
T17850	4032-4043	NN	denotes	brightfield
T17849	4028-4031	CC	denotes	and
T17848	4018-4027	NN	denotes	Darkfield
T17847	4013-4016	NN	denotes	33P
T17846	4008-4012	IN	denotes	with
T17845	4000-4007	VB	denotes	labeled
T17844	3993-3999	NN	denotes	probes
T17843	3989-3992	NN	denotes	RNA
T17842	3977-3988	VB	denotes	transcribed
T17841	3971-3976	FW	denotes	vitro
T17840	3968-3970	FW	denotes	in
T17839	3962-3967	VB	denotes	using
T17838	3960-3961	-RRB-	denotes	]
T17837	3958-3960	CD	denotes	52
T17836	3957-3958	-LRB-	denotes	[
T17835	3947-3956	VB	denotes	described
T17834	3944-3946	IN	denotes	as
T17833	3930-3943	NN	denotes	hybridization
T17832	3925-3929	FW	denotes	situ
T17831	3922-3924	FW	denotes	in
T17830	3918-3921	IN	denotes	for
T17829	3908-3917	VB	denotes	processed
T18190	4586-4589	CC	denotes	and
T18189	4572-4585	VB	denotes	exsanguinated
T18188	4567-4571	VB	denotes	were
T18187	4559-4566	NN	denotes	embryos
T18186	4550-4558	JJ	denotes	18.5-dpc
T18185	4539-4548	NNP	denotes	Histology
T17394	3531-3532	-RRB-	denotes	)
T17393	3525-3531	NNP	denotes	States
T17392	3518-3524	NNP	denotes	United
T17391	3516-3517	-COMMA-	denotes	,
T17390	3506-3516	NNP	denotes	Washington
T17389	3504-3505	-COMMA-	denotes	,
T17388	3497-3504	NNP	denotes	Redmond
T17387	3496-3497	-LRB-	denotes	(
T17386	3490-3495	NNP	denotes	Excel
T17385	3480-3489	NNP	denotes	Microsoft
T17384	3477-3479	IN	denotes	in
T17383	3471-3476	NN	denotes	GAPDH
T17382	3468-3470	TO	denotes	to
T17381	3457-3467	VB	denotes	normalized
T17380	3453-3456	CC	denotes	and
T17379	3451-3452	-RRB-	denotes	)
T17378	3441-3451	NNP	denotes	Biosystems
T17377	3433-3440	NNP	denotes	Applied
T17376	3432-3433	-LRB-	denotes	(
T17375	3423-3431	NNP	denotes	Software
T17374	3419-3422	NNP	denotes	SDS
T17373	3414-3418	CD	denotes	7000
T17372	3408-3413	NNP	denotes	Prism
T17371	3404-3407	NNP	denotes	ABI
T17370	3400-3403	DT	denotes	the
T17369	3397-3399	IN	denotes	in
T17368	3386-3396	VB	denotes	calculated
T17367	3381-3385	VB	denotes	were
T17366	3374-3380	NN	denotes	values
T17365	3361-3373	JJ	denotes	quantitative
T17364	3352-3360	JJ	denotes	Relative
T17363	3342-3350	NN	denotes	reaction
T17362	3338-3341	NN	denotes	PCR
T17361	3333-3337	DT	denotes	each
T17360	3329-3332	IN	denotes	for
T17359	3324-3328	VB	denotes	done
T17358	3319-3323	VB	denotes	were
T17357	3307-3318	NN	denotes	Triplicates
T17356	3291-3305	NN	denotes	amplifications
T17355	3287-3290	DT	denotes	the
T17354	3284-3286	IN	denotes	of
T17353	3273-3283	NN	denotes	efficiency
T17352	3269-3272	DT	denotes	the
T17351	3264-3268	VB	denotes	test
T17350	3261-3263	TO	denotes	to
T17349	3251-3260	VB	denotes	performed
T17348	3246-3250	VB	denotes	were
T17347	3237-3245	NN	denotes	analyses
T17346	3231-3236	NN	denotes	curve
T17345	3222-3230	JJ	denotes	Standard
T17344	3217-3220	NN	denotes	RNA
T17343	3211-3216	NN	denotes	input
T17342	3207-3210	IN	denotes	for
T17341	3199-3206	NN	denotes	control
T17340	3196-3198	IN	denotes	as
T17339	3191-3195	VB	denotes	used
T17338	3186-3190	VB	denotes	were
T17337	3179-3185	NN	denotes	levels
T17336	3174-3178	NN	denotes	mRNA
T17335	3168-3173	NN	denotes	GAPDH
T17334	3158-3166	NN	denotes	supermix
T17333	3152-3157	JJ	denotes	green
T17332	3147-3151	NN	denotes	SYBR
T17331	3144-3146	NN	denotes	μl
T17330	3139-3143	CD	denotes	12.5
T17329	3134-3138	IN	denotes	with
T17328	3125-3133	NN	denotes	reaction
T17327	3119-3124	JJ	denotes	25-μl
T17326	3117-3118	DT	denotes	a
T17325	3114-3116	IN	denotes	in
T17324	3104-3113	VB	denotes	performed
T17323	3100-3103	VB	denotes	was
T17322	3096-3099	NN	denotes	PCR
T17321	3092-3095	DT	denotes	All
T17320	3082-3090	NN	denotes	facility
T17319	3077-3081	NN	denotes	core
T17318	3069-3076	NNP	denotes	Arizona
T17317	3066-3068	IN	denotes	of
T17316	3055-3065	NNP	denotes	University
T17315	3051-3054	DT	denotes	the
T17314	3048-3050	IN	denotes	at
T17313	3046-3047	-RRB-	denotes	)
T17312	3040-3046	NNP	denotes	States
T17311	3033-3039	NNP	denotes	United
T17310	3031-3032	-COMMA-	denotes	,
T17309	3021-3031	NNP	denotes	California
T17308	3019-3020	-COMMA-	denotes	,
T17307	3015-3019	NNP	denotes	City
T17306	3008-3014	NNP	denotes	Foster
T17305	3006-3007	-COMMA-	denotes	,
T17304	2996-3006	NNP	denotes	Biosystems
T17828	3903-3907	VB	denotes	were
T17827	3896-3902	NN	denotes	Slides
T17826	3893-3894	-RRB-	denotes	)
T17825	3887-3893	NNP	denotes	States
T17824	3880-3886	NNP	denotes	United
T17823	3878-3879	-COMMA-	denotes	,
T17822	3866-3878	NNP	denotes	Pennsylvania
T17821	3864-3865	-COMMA-	denotes	,
T17820	3857-3864	NNP	denotes	Chester
T17819	3852-3856	NNP	denotes	West
T17818	3850-3851	-COMMA-	denotes	,
T17817	3847-3850	NN	denotes	VWR
T17816	3846-3847	-LRB-	denotes	(
T17815	3839-3845	NN	denotes	slides
T17814	3830-3838	VB	denotes	modified
T17813	3823-3829	VB	denotes	charge
T17812	3820-3822	TO	denotes	to
T17811	3812-3819	VB	denotes	adhered
T17810	3808-3811	CC	denotes	and
T17809	3806-3807	-COMMA-	denotes	,
T17808	3804-3806	NN	denotes	μm
T17807	3802-3803	CD	denotes	5
T17806	3799-3801	IN	denotes	at
T17805	3789-3798	VB	denotes	sectioned
T17804	3787-3788	-COMMA-	denotes	,
T17803	3779-3787	NN	denotes	paraffin
T17802	3776-3778	IN	denotes	in
T17801	3767-3775	VB	denotes	embedded
T17800	3765-3766	-COMMA-	denotes	,
T17799	3749-3765	NN	denotes	paraformaldehyde
T17798	3747-3748	NN	denotes	%
T17797	3746-3747	CD	denotes	4
T17796	3743-3745	IN	denotes	in
T17795	3733-3742	NN	denotes	immersion
T17794	3730-3732	IN	denotes	by
T17793	3720-3729	RB	denotes	overnight
T17792	3714-3719	VB	denotes	fixed
T17791	3709-3713	VB	denotes	were
T17790	3701-3708	NN	denotes	Embryos
T17789	3690-3699	CD	denotes	1353–1927
T17788	3678-3689	NN	denotes	nucleotides
T17787	3673-3677	NN	denotes	Sox6
T17786	3667-3672	NN	denotes	mouse
T17785	3663-3666	CC	denotes	and
T17784	3661-3662	-COLON-	denotes	;
T17783	3654-3661	CD	denotes	458–549
T17782	3642-3653	NN	denotes	nucleotides
T17781	3635-3641	NN	denotes	globin
T17780	3630-3634	SYM	denotes	βmaj
T17779	3628-3629	-COLON-	denotes	;
T17778	3621-3628	CD	denotes	509–584
T17777	3609-3620	NN	denotes	nucleotides
T17776	3602-3608	NN	denotes	globin
T17775	3599-3601	JJ	denotes	ɛy
T17774	3592-3598	JJ	denotes	murine
T17773	3589-3591	TO	denotes	to
T17772	3580-3588	VB	denotes	designed
T17771	3575-3579	VB	denotes	were
T17770	3568-3574	NN	denotes	probes
T17769	3558-3567	JJ	denotes	Antisense
T17768	3543-3556	NN	denotes	hybridization
T17767	3538-3542	FW	denotes	situ
T17766	3535-3537	FW	denotes	In
T17303	2988-2995	NNP	denotes	Applied
T17302	2987-2988	-LRB-	denotes	(
T17301	2979-2986	NN	denotes	ABI7000
T17300	2976-2978	DT	denotes	an
T17299	2973-2975	IN	denotes	on
T17298	2969-2972	VB	denotes	run
T17297	2965-2968	VB	denotes	was
T17296	2951-2964	NN	denotes	amplification
T17295	2947-2950	NN	denotes	PCR
T17294	2945-2946	-COMMA-	denotes	,
T17293	2944-2945	-RRB-	denotes	)
T17292	2938-2944	NNP	denotes	States
T17291	2931-2937	NNP	denotes	United
T17290	2929-2930	-COMMA-	denotes	,
T17289	2919-2929	NNP	denotes	California
T17288	2917-2918	-COMMA-	denotes	,
T17287	2909-2917	NNP	denotes	Hercules
T17286	2907-2908	-COMMA-	denotes	,
T17285	2900-2907	NNP	denotes	Bio-Rad
T17284	2899-2900	-LRB-	denotes	(
T17283	2895-2898	NN	denotes	ROX
T17282	2890-2894	IN	denotes	with
T17281	2886-2889	NN	denotes	kit
T17280	2877-2885	NN	denotes	supermix
T17279	2871-2876	JJ	denotes	green
T17278	2866-2870	NN	denotes	SYBR
T17277	2862-2865	DT	denotes	the
T17276	2856-2861	VB	denotes	Using
T17275	2830-2854	NN	denotes	5′ACGATCATATTGCCCAGGAG3′
T17274	2828-2829	-COMMA-	denotes	,
T17273	2821-2828	NN	denotes	MHB1675
T17272	2817-2820	CC	denotes	and
T17271	2815-2816	-COLON-	denotes	;
T17270	2791-2815	NN	denotes	5′ATGGCCTGAATCACTTGGAC3′
T17269	2789-2790	-COLON-	denotes	:
T17268	2782-2789	NN	denotes	MHB1674
T17267	2780-2781	-COLON-	denotes	:
T17266	2774-2780	NN	denotes	globin
T17265	2765-2773	NN	denotes	βmaj/min
T17264	2761-2764	IN	denotes	For
T17263	2735-2759	NN	denotes	5′ACCTCTGGGGTGAATTCCTT3′
T17262	2733-2734	-COMMA-	denotes	,
T17261	2726-2733	NN	denotes	MHB1673
T17260	2722-2725	CC	denotes	and
T17259	2720-2721	-COLON-	denotes	;
T17258	2696-2720	NN	denotes	5′TGGACAACCTCAAGGAGACC3′
T17257	2694-2695	-COMMA-	denotes	,
T17256	2687-2694	NN	denotes	MHB1672
T17255	2685-2686	-COLON-	denotes	:
T17254	2679-2685	NN	denotes	globin
T17253	2675-2678	NN	denotes	βH1
T17252	2671-2674	IN	denotes	For
T17251	2645-2669	NN	denotes	5′CTTAACCGCATCCCCTACGG3′
T17250	2643-2644	-COMMA-	denotes	,
T17249	2636-2643	NN	denotes	MHB1669
T17248	2632-2635	CC	denotes	and
T17247	2630-2631	-COLON-	denotes	;
T17246	2605-2630	NN	denotes	5′CTACCCCCAGACGAAGACCTA3′
T17245	2603-2604	-COMMA-	denotes	,
T17244	2596-2603	NN	denotes	MHB1668
T17243	2594-2595	-COLON-	denotes	:
T17242	2588-2594	NN	denotes	globin
T17241	2586-2587	NN	denotes	ζ
T17240	2582-2585	IN	denotes	For
T17239	2555-2580	NN	denotes	5′GAAGCAGAGGACAAGTTCCCA3′
T17238	2553-2554	-COMMA-	denotes	,
T17237	2546-2553	NN	denotes	MHB1667
T17236	2542-2545	CC	denotes	and
T17235	2540-2541	-COLON-	denotes	;
T17234	2515-2540	NN	denotes	5′TGGCCTGTGGAGTAAGGTCAA3′
T17233	2513-2514	-COMMA-	denotes	,
T17232	2506-2513	NN	denotes	MHB1666
T17231	2504-2505	-COLON-	denotes	:
T17230	2498-2504	NN	denotes	globin
T17229	2495-2497	JJ	denotes	ɛy
T17228	2491-2494	IN	denotes	For
T17227	2478-2489	NN	denotes	specificity
T17226	2470-2477	VB	denotes	confirm
T17225	2467-2469	TO	denotes	to
T17224	2458-2466	NN	denotes	database
T17223	2453-2457	NN	denotes	NCBI
T17222	2449-2452	DT	denotes	the
T17221	2441-2448	IN	denotes	against
T17220	2432-2440	VB	denotes	searched
T17219	2427-2431	VB	denotes	were
T17218	2419-2426	NN	denotes	primers
T17217	2415-2418	DT	denotes	All
T17216	2412-2413	-RRB-	denotes	]
T17215	2410-2412	CD	denotes	51
T17214	2409-2410	-LRB-	denotes	[
T17213	2398-2408	NNP	denotes	Primerbank
T17212	2393-2397	IN	denotes	from
T17211	2384-2392	VB	denotes	obtained
T17210	2379-2383	VB	denotes	were
T17209	2373-2378	NN	denotes	genes
T17208	2366-2372	NN	denotes	globin
T17207	2363-2365	IN	denotes	of
T17206	2349-2362	NN	denotes	amplification
T17205	2345-2348	NN	denotes	PCR
T17204	2340-2344	NN	denotes	cDNA
T17203	2336-2339	IN	denotes	for
T17202	2328-2335	NN	denotes	Primers
T17201	2322-2326	NN	denotes	cDNA
T17200	2319-2321	TO	denotes	to
T17199	2307-2318	VB	denotes	transcribed
T17198	2299-2306	JJ	denotes	reverse
T17197	2293-2298	RB	denotes	first
T17196	2289-2292	VB	denotes	was
T17195	2285-2288	NN	denotes	RNA
T17194	2279-2283	NN	denotes	mRNA
T17193	2272-2278	NN	denotes	globin
T17192	2269-2271	IN	denotes	of
T17191	2256-2268	NN	denotes	Quantitation
T16443	2252-2253	-RRB-	denotes	)
T16442	2249-2252	CD	denotes	−18
T16441	2246-2248	TO	denotes	to
T16440	2242-2245	CD	denotes	−63
T16439	2241-2242	-LRB-	denotes	(
T16438	2238-2240	NN	denotes	3′
T16437	2189-2237	NN	denotes	5′CCGCTCGAGAATGCATGCCAAGGGTCAGATGAGTGTCTGCGAAGAA
T16436	2187-2188	-COMMA-	denotes	,
T16435	2180-2187	NN	denotes	MHB1663
T16434	2176-2179	CC	denotes	and
T16433	2174-2175	-COLON-	denotes	;
T16432	2173-2174	-RRB-	denotes	)
T16431	2170-2173	CD	denotes	−16
T16430	2167-2169	TO	denotes	to
T16429	2163-2166	CD	denotes	−63
T16428	2162-2163	-LRB-	denotes	(
T16427	2110-2161	NN	denotes	5′CCGCTCGAGAATGCAGAACAAAGGGTCAGATGAGTGTCTGCGAAGAA3′
T16426	2108-2109	-COMMA-	denotes	,
T16425	2101-2108	NN	denotes	MHB1662
T16424	2099-2100	-COLON-	denotes	;
T16423	2098-2099	-RRB-	denotes	)
T16422	2095-2098	CD	denotes	−19
T16421	2092-2094	TO	denotes	to
T16420	2088-2091	CD	denotes	−63
T16184	665-669	WDT	denotes	that
T16183	659-664	NN	denotes	sites
T16182	651-658	NN	denotes	HindIII
T16181	647-650	CC	denotes	and
T16180	642-646	NN	denotes	XhoI
T16179	632-641	VB	denotes	contained
T16178	624-631	NN	denotes	primers
T16177	620-623	NN	denotes	PCR
T16176	616-619	DT	denotes	The
T16175	606-614	NN	denotes	database
T16174	604-605	-RRB-	denotes	)
T16173	599-604	NN	denotes	Mouse
T16172	596-598	IN	denotes	of
T16171	585-595	NN	denotes	Annotation
T16170	574-584	JJ	denotes	Functional
T16169	573-574	-LRB-	denotes	(
T16168	566-572	NNP	denotes	Fantom
T16167	562-565	DT	denotes	the
T16166	559-561	IN	denotes	in
T16165	554-558	NN	denotes	cDNA
T16164	546-553	JJ	denotes	longest
T16163	542-545	DT	denotes	the
T16162	539-541	IN	denotes	on
T16161	533-538	VB	denotes	based
T16160	530-532	VB	denotes	is
T16159	525-529	NN	denotes	site
T16158	519-524	NN	denotes	start
T16157	503-518	JJ	denotes	transcriptional
T16156	499-502	DT	denotes	The
T16155	493-497	NN	denotes	site
T16154	487-492	NN	denotes	start
T16153	473-486	NN	denotes	transcription
T16152	469-472	DT	denotes	the
T16151	466-468	TO	denotes	to
T16150	457-465	JJ	denotes	relative
T16149	454-456	VB	denotes	is
T16148	444-453	NN	denotes	numbering
T16147	433-443	NN	denotes	Nucleotide
T16146	430-431	-RRB-	denotes	]
T16145	428-430	CD	denotes	50
T16144	427-428	-LRB-	denotes	[
T16143	422-426	NN	denotes	site
T16142	418-421	NN	denotes	cap
T16141	413-417	NN	denotes	gene
T16140	406-412	NN	denotes	globin
T16139	404-405	NN	denotes	ɛ
T16138	400-403	DT	denotes	the
T16137	397-399	IN	denotes	of
T16136	388-396	RB	denotes	upstream
T16135	379-387	NN	denotes	sequence
T16134	376-378	IN	denotes	of
T16133	373-375	NN	denotes	kb
T16132	371-372	CD	denotes	2
T16131	365-370	IN	denotes	about
T16130	354-364	VB	denotes	containing
T16129	345-353	NN	denotes	fragment
T16128	339-344	NN	denotes	EcoRI
T16127	332-338	JJ	denotes	3.7-kb
T16126	330-331	DT	denotes	a
T16125	323-329	IN	denotes	within
T16124	315-322	JJ	denotes	located
T16123	311-314	VB	denotes	are
T16122	301-310	NN	denotes	silencing
T16121	296-300	NN	denotes	gene
T16120	294-295	NN	denotes	ɛ
T16119	290-293	IN	denotes	for
T16118	281-289	VB	denotes	required
T16117	271-280	NN	denotes	sequences
T16116	267-270	DT	denotes	all
T16115	262-266	IN	denotes	that
T16114	256-261	VB	denotes	shown
T16113	251-255	VB	denotes	been
T16112	247-250	VB	denotes	has
T16111	244-246	PRP	denotes	it
T16110	236-243	IN	denotes	because
T16109	234-235	-COMMA-	denotes	,
T16108	230-234	VB	denotes	used
T16107	226-229	VB	denotes	was
T16106	224-225	-RRB-	denotes	)
T16105	221-224	NN	denotes	ATG
T16104	220-221	-LRB-	denotes	(
T16103	214-219	NN	denotes	codon
T16102	203-213	NN	denotes	initiation
T16101	196-202	NN	denotes	globin
T16100	193-195	JJ	denotes	ɛy
T16099	189-192	DT	denotes	the
T16098	186-188	IN	denotes	of
T16097	177-185	RB	denotes	upstream
T16096	168-176	NN	denotes	fragment
T16095	161-167	JJ	denotes	2.2-kb
T16094	159-160	DT	denotes	A
T16093	149-157	NN	denotes	promoter
T16092	140-148	JJ	denotes	proximal
T16091	137-139	JJ	denotes	ɛy
T16090	133-136	DT	denotes	the
T16089	130-132	IN	denotes	of
T16088	116-129	NN	denotes	amplification
T16087	112-115	NN	denotes	PCR
T16086	109-111	IN	denotes	by
T16085	99-108	VB	denotes	generated
T16084	95-98	VB	denotes	was
T16083	93-94	-RRB-	denotes	)
T16082	88-93	NN	denotes	E-luc
T16081	87-88	-LRB-	denotes	(
T16080	79-86	NN	denotes	plasmid
T16079	70-78	NN	denotes	reporter
T16078	61-69	NN	denotes	deletion
T16077	52-60	NN	denotes	promoter
T16076	49-51	JJ	denotes	ɛy
T16075	45-48	DT	denotes	The
T16074	31-43	NN	denotes	construction
T16073	23-30	NN	denotes	Plasmid
T16374	1781-1785	WDT	denotes	that
T16373	1779-1780	-RRB-	denotes	)
T16372	1761-1779	NN	denotes	Sox6-ΔHMG-pcDNA3.1
T16371	1760-1761	-LRB-	denotes	(
T16370	1750-1759	NN	denotes	construct
T16369	1735-1749	NN	denotes	overexpression
T16368	1730-1734	NN	denotes	Sox6
T16367	1726-1729	DT	denotes	the
T16366	1723-1725	IN	denotes	of
T16365	1715-1722	NN	denotes	version
T16364	1705-1714	VB	denotes	truncated
T16363	1703-1704	DT	denotes	A
T16362	1697-1701	NN	denotes	Sox6
T16361	1685-1696	VB	denotes	overexpress
T16360	1682-1684	TO	denotes	to
T16359	1677-1681	VB	denotes	used
T16358	1673-1676	VB	denotes	was
T16357	1671-1672	-RRB-	denotes	]
T16356	1669-1671	CD	denotes	15
T16355	1668-1669	-LRB-	denotes	[
T16354	1654-1667	NN	denotes	Sox6-pcDNA3.1
T16353	1651-1652	-RRB-	denotes	)
T16352	1648-1651	CD	denotes	+20
T16351	1645-1647	TO	denotes	to
T16350	1641-1644	CD	denotes	+45
T16349	1640-1641	-LRB-	denotes	(
T16348	1610-1639	NN	denotes	5′CGGAAGCTTGGGAGGTTGCTGGTGA3′
T16347	1608-1609	-COMMA-	denotes	,
T16346	1601-1608	NN	denotes	MHB1477
T16345	1599-1600	-COLON-	denotes	:
T16344	1595-1599	NN	denotes	site
T16343	1587-1594	NN	denotes	HindIII
T16342	1580-1586	NN	denotes	primer
T16341	1572-1579	JJ	denotes	reverse
T16340	1568-1571	DT	denotes	the
T16339	1563-1567	IN	denotes	with
T16338	1551-1562	NN	denotes	combination
T16337	1548-1550	IN	denotes	in
T16336	1543-1547	VB	denotes	used
T16335	1538-1542	VB	denotes	were
T16334	1530-1537	NN	denotes	primers
T16333	1522-1529	JJ	denotes	forward
T16332	1518-1521	DT	denotes	All
T16331	1515-1516	-RRB-	denotes	)
T16330	1513-1515	CD	denotes	−9
T16329	1510-1512	TO	denotes	to
T16328	1506-1509	CD	denotes	−37
T16327	1505-1506	-LRB-	denotes	(
T16326	1502-1504	NN	denotes	3′
T16325	1470-1501	NN	denotes	5′CCGCTCGAGGTCTGCGAAGAATAAAAGGC
T16324	1468-1469	-COMMA-	denotes	,
T16323	1461-1468	NN	denotes	MHB1507
T16322	1457-1460	CC	denotes	and
T16321	1455-1456	-COLON-	denotes	;
T16320	1454-1455	-RRB-	denotes	)
T16319	1451-1454	CD	denotes	−34
T16318	1448-1450	TO	denotes	to
T16317	1444-1447	CD	denotes	−63
T16316	1443-1444	-LRB-	denotes	(
T16315	1408-1442	NN	denotes	5′CCGCTCGAGAATGCAGAACAAAGGGTCAGA3′
T16314	1406-1407	-COMMA-	denotes	,
T16313	1399-1406	NN	denotes	MHB1532
T16312	1397-1398	-COLON-	denotes	;
T16311	1396-1397	-RRB-	denotes	)
T16310	1393-1396	CD	denotes	−58
T16309	1390-1392	TO	denotes	to
T16308	1386-1389	CD	denotes	−85
T16307	1385-1386	-LRB-	denotes	(
T16306	1352-1384	NN	denotes	5′CCGCTCGAGCTGACCAATGGCTTCAAAG3′
T16305	1350-1351	-COMMA-	denotes	,
T16304	1343-1350	NN	denotes	MHB1506
T16303	1341-1342	-COLON-	denotes	;
T16302	1340-1341	-RRB-	denotes	)
T16301	1336-1340	CD	denotes	−169
T16300	1333-1335	TO	denotes	to
T16299	1328-1332	CD	denotes	−197
T16298	1327-1328	-LRB-	denotes	(
T16297	1294-1326	NN	denotes	5′CCGCTCGAGGGAGCCAAAAAAAGAATGC3′
T16296	1292-1293	-COMMA-	denotes	,
T16295	1285-1292	NN	denotes	MHB1505
T16294	1283-1284	-COLON-	denotes	;
T16293	1282-1283	-RRB-	denotes	)
T16292	1278-1282	CD	denotes	−605
T16291	1275-1277	TO	denotes	to
T16290	1270-1274	CD	denotes	−634
T16289	1269-1270	-LRB-	denotes	(
T16288	1234-1268	NN	denotes	5′CCGCTCGAGTCTCTACACTGTCACTCCCTG3′
T16287	1232-1233	-COMMA-	denotes	,
T16286	1225-1232	NN	denotes	MHB1503
T16285	1223-1224	-COLON-	denotes	;
T16284	1222-1223	-RRB-	denotes	)
T16283	1217-1222	CD	denotes	−2052
T16282	1214-1216	TO	denotes	to
T16281	1208-1213	CD	denotes	−2077
T16280	1207-1208	-LRB-	denotes	(
T16279	1176-1206	NN	denotes	5′CCGCTCGAGTGCTAGGCAAACACTCA3′
T16278	1174-1175	-COMMA-	denotes	,
T16277	1167-1174	NN	denotes	MHB1457
T16276	1165-1166	-COLON-	denotes	:
T16275	1158-1165	VB	denotes	include
T16274	1153-1157	NN	denotes	site
T16273	1148-1152	NN	denotes	XhoI
T16272	1145-1147	DT	denotes	an
T16271	1140-1144	IN	denotes	with
T16270	1132-1139	NN	denotes	primers
T16269	1124-1131	JJ	denotes	Forward
T16268	1113-1122	RB	denotes	similarly
T16267	1103-1112	VB	denotes	generated
T16266	1098-1102	VB	denotes	were
T16265	1089-1097	NN	denotes	promoter
T16264	1086-1088	JJ	denotes	ɛy
T16263	1082-1085	DT	denotes	the
T16262	1079-1081	IN	denotes	of
T16261	1068-1078	NN	denotes	constructs
T16260	1059-1067	NN	denotes	deletion
T16259	1056-1058	IN	denotes	of
T16258	1049-1055	NN	denotes	series
T16257	1047-1048	DT	denotes	A
T16256	1037-1045	NN	denotes	promoter
T16255	1034-1036	JJ	denotes	ɛy
T16254	1030-1033	DT	denotes	the
T16253	1027-1029	IN	denotes	by
T16252	1020-1026	VB	denotes	driven
T16251	1017-1019	VB	denotes	is
T16250	1006-1016	NN	denotes	expression
T16249	995-1005	NN	denotes	luciferase
T16248	989-994	WDT	denotes	which
T16247	986-988	IN	denotes	in
T16246	976-985	NN	denotes	construct
T16245	967-975	NN	denotes	reporter
T16244	965-966	DT	denotes	a
T16243	962-964	IN	denotes	in
T16242	952-961	VB	denotes	resulting
T16241	950-951	-COMMA-	denotes	,
T16240	943-950	NN	denotes	plasmid
T16239	937-942	JJ	denotes	Basic
T16238	932-936	NN	denotes	pGL3
T16237	926-931	JJ	denotes	above
T16236	922-925	DT	denotes	the
T16235	919-921	IN	denotes	in
T16234	910-918	NN	denotes	promoter
T16233	907-909	JJ	denotes	ɛy
T16232	903-906	DT	denotes	the
T16231	900-902	IN	denotes	of
T16230	891-899	RB	denotes	upstream
T16229	882-890	VB	denotes	inserted
T16228	877-881	RB	denotes	then
T16227	873-876	VB	denotes	was
T16226	871-872	-RRB-	denotes	)
T16225	870-871	-RRB-	denotes	]
T16224	868-870	CD	denotes	31
T16223	867-868	-LRB-	denotes	[
T16222	865-866	-COLON-	denotes	;
T16221	862-865	NN	denotes	LCR
T16220	856-861	NN	denotes	micro
T16219	855-856	-LRB-	denotes	(
T16218	851-854	CD	denotes	9.3
T16217	845-850	NN	denotes	μLCRβ
T16216	842-844	IN	denotes	of
T16215	833-841	NN	denotes	fragment
T16214	823-832	NN	denotes	SstI/XhoI
T16213	816-822	JJ	denotes	2.5-kb
T16212	814-815	DT	denotes	A
T16211	811-812	-RRB-	denotes	)
T16210	805-811	NNP	denotes	States
T16209	798-804	NNP	denotes	United
T16208	796-797	-COMMA-	denotes	,
T16207	787-796	NNP	denotes	Wisconsin
T16206	785-786	-COMMA-	denotes	,
T16205	778-785	NNP	denotes	Madison
T16204	776-777	-COMMA-	denotes	,
T16203	769-776	NNP	denotes	Promega
T16202	768-769	-LRB-	denotes	(
T16201	762-767	JJ	denotes	Basic
T16200	757-761	NN	denotes	pGL3
T16199	754-756	IN	denotes	in
T16198	749-753	NN	denotes	gene
T16197	738-748	NN	denotes	luciferase
T16196	730-737	NN	denotes	firefly
T16195	726-729	DT	denotes	the
T16194	723-725	IN	denotes	of
T16193	714-722	RB	denotes	upstream
T16192	705-713	NN	denotes	fragment
T16191	696-704	NN	denotes	promoter
T16190	693-695	JJ	denotes	ɛy
T16189	689-692	DT	denotes	the
T16188	683-688	VB	denotes	clone
T16187	680-682	TO	denotes	to
T16186	675-679	VB	denotes	used
T16185	670-674	VB	denotes	were
T16419	2087-2088	-LRB-	denotes	(
T16418	2037-2086	NN	denotes	5′CCGCTCGAGAATGCAGTGCCAAGGGTCAGAACATTGTCTGCGAAG3′
T16417	2035-2036	-COMMA-	denotes	,
T16416	2028-2035	NN	denotes	MHB1661
T16415	2026-2027	-COLON-	denotes	:
T16414	2019-2026	VB	denotes	include
T16413	2008-2018	NN	denotes	constructs
T16412	1999-2007	NN	denotes	reporter
T16411	1990-1998	NN	denotes	promoter
T16410	1987-1989	NN	denotes	ɛy
T16409	1975-1986	VB	denotes	mutagenized
T16408	1969-1974	DT	denotes	these
T16407	1960-1968	VB	denotes	generate
T16406	1957-1959	TO	denotes	to
T16405	1952-1956	VB	denotes	used
T16404	1944-1951	NN	denotes	primers
T16403	1936-1943	JJ	denotes	Forward
T16402	1931-1934	NN	denotes	PCR
T16401	1928-1930	IN	denotes	by
T16400	1923-1927	VB	denotes	done
T16399	1918-1922	VB	denotes	were
T16398	1909-1917	NN	denotes	promoter
T16397	1906-1908	JJ	denotes	ɛy
T16396	1902-1905	DT	denotes	the
T16395	1899-1901	IN	denotes	of
T16394	1893-1898	NN	denotes	sites
T16393	1885-1892	NN	denotes	binding
T16392	1875-1884	NN	denotes	consensus
T16391	1866-1874	NN	denotes	Sox/Sox6
T16390	1863-1865	IN	denotes	of
T16389	1851-1862	NN	denotes	Mutagenesis
T16388	1848-1849	-RRB-	denotes	]
T16387	1846-1848	CD	denotes	32
T16386	1845-1846	-LRB-	denotes	[
T16385	1838-1844	NN	denotes	others
T16384	1835-1837	IN	denotes	by
T16383	1825-1834	VB	denotes	described
T16382	1822-1824	IN	denotes	as
T16381	1820-1821	-COMMA-	denotes	,
T16380	1811-1820	VB	denotes	generated
T16379	1807-1810	VB	denotes	was
T16378	1800-1806	NN	denotes	domain
T16377	1796-1799	NN	denotes	HMG
T16376	1792-1795	DT	denotes	the
T16375	1786-1791	VB	denotes	lacks
T19875	8522-8526	VB	denotes	used
T19200	6872-6881	VB	denotes	described
R11081	T16074	T16073	arg1Of	construction,Plasmid
R11082	T16080	T16075	arg1Of	plasmid,The
R11083	T16080	T16076	arg1Of	plasmid,ɛy
R11084	T16080	T16077	arg1Of	plasmid,promoter
R11085	T16080	T16078	arg1Of	plasmid,deletion
R11086	T16080	T16079	arg1Of	plasmid,reporter
R11087	T16080	T16081	arg1Of	plasmid,(
R11088	T16080	T16084	arg1Of	plasmid,was
R11089	T16080	T16085	arg2Of	plasmid,generated
R11090	T16082	T16081	arg2Of	E-luc,(
R11091	T16083	T16081	arg3Of	),(
R11092	T16085	T16084	arg2Of	generated,was
R11093	T16088	T16085	arg1Of	amplification,generated
R11094	T16088	T16086	arg2Of	amplification,by
R11095	T16088	T16087	arg1Of	amplification,PCR
R11096	T16088	T16089	arg1Of	amplification,of
R11097	T16093	T16089	arg2Of	promoter,of
R11098	T16093	T16090	arg1Of	promoter,the
R11099	T16093	T16091	arg1Of	promoter,ɛy
R11100	T16093	T16092	arg1Of	promoter,proximal
R11101	T16096	T16094	arg1Of	fragment,A
R11102	T16096	T16095	arg1Of	fragment,2.2-kb
R11103	T16096	T16097	arg1Of	fragment,upstream
R11104	T16096	T16107	arg1Of	fragment,was
R11105	T16096	T16108	arg2Of	fragment,used
R11106	T16097	T16098	arg1Of	upstream,of
R11107	T16103	T16098	arg2Of	codon,of
R11108	T16103	T16099	arg1Of	codon,the
R11109	T16103	T16100	arg1Of	codon,ɛy
R11110	T16103	T16101	arg1Of	codon,globin
R11111	T16103	T16102	arg1Of	codon,initiation
R11112	T16103	T16104	arg1Of	codon,(
R11113	T16105	T16104	arg2Of	ATG,(
R11114	T16106	T16104	arg3Of	),(
R11115	T16108	T16107	arg2Of	used,was
R11116	T16108	T16109	arg1Of	used,","
R11117	T16108	T16110	arg1Of	used,because
R11118	T16111	T16112	arg1Of	it,has
R11119	T16111	T16113	arg1Of	it,been
R11120	T16111	T16114	arg2Of	it,shown
R11121	T16114	T16110	arg2Of	shown,because
R11122	T16114	T16112	arg2Of	shown,has
R11123	T16114	T16113	arg2Of	shown,been
R11124	T16117	T16116	arg1Of	sequences,all
R11125	T16117	T16118	arg2Of	sequences,required
R11126	T16117	T16123	arg1Of	sequences,are
R11127	T16117	T16124	arg1Of	sequences,located
R11128	T16118	T16119	arg1Of	required,for
R11129	T16122	T16119	arg2Of	silencing,for
R11130	T16122	T16120	arg1Of	silencing,ɛ
R11131	T16122	T16121	arg1Of	silencing,gene
R11132	T16123	T16114	arg3Of	are,shown
R11133	T16123	T16115	arg1Of	are,that
R11134	T16124	T16123	arg2Of	located,are
R11135	T16124	T16125	arg1Of	located,within
R11136	T16129	T16125	arg2Of	fragment,within
R11137	T16129	T16126	arg1Of	fragment,a
R11138	T16129	T16127	arg1Of	fragment,3.7-kb
R11139	T16129	T16128	arg1Of	fragment,EcoRI
R11140	T16129	T16130	arg1Of	fragment,containing
R11141	T16130	T16144	arg1Of	containing,[
R11142	T16132	T16131	arg1Of	2,about
R11143	T16133	T16130	arg2Of	kb,containing
R11144	T16133	T16132	arg1Of	kb,2
R11145	T16133	T16134	arg1Of	kb,of
R11146	T16133	T16136	arg1Of	kb,upstream
R11147	T16135	T16134	arg2Of	sequence,of
R11148	T16136	T16137	arg1Of	upstream,of
R11149	T16143	T16137	arg2Of	site,of
R11150	T16143	T16138	arg1Of	site,the
R11151	T16143	T16139	arg1Of	site,ɛ
R11152	T16143	T16140	arg1Of	site,globin
R11153	T16143	T16141	arg1Of	site,gene
R11154	T16143	T16142	arg1Of	site,cap
R11155	T16145	T16144	arg2Of	50,[
R11156	T16146	T16144	arg3Of	],[
R11157	T16148	T16147	arg1Of	numbering,Nucleotide
R11158	T16148	T16149	arg1Of	numbering,is
R11159	T16148	T16150	arg1Of	numbering,relative
R11160	T16150	T16149	arg2Of	relative,is
R11161	T16150	T16151	arg1Of	relative,to
R11162	T16155	T16151	arg2Of	site,to
R11163	T16155	T16152	arg1Of	site,the
R11164	T16155	T16153	arg1Of	site,transcription
R11165	T16155	T16154	arg1Of	site,start
R11166	T16159	T16156	arg1Of	site,The
R11167	T16159	T16157	arg1Of	site,transcriptional
R11168	T16159	T16158	arg1Of	site,start
R11169	T16159	T16160	arg1Of	site,is
R11170	T16159	T16161	arg2Of	site,based
R11171	T16161	T16160	arg2Of	based,is
R11172	T16161	T16162	arg1Of	based,on
R11173	T16165	T16162	arg2Of	cDNA,on
R11174	T16165	T16163	arg1Of	cDNA,the
R11175	T16165	T16164	arg1Of	cDNA,longest
R11176	T16165	T16166	arg1Of	cDNA,in
R11177	T16168	T16169	arg1Of	Fantom,(
R11178	T16171	T16169	arg2Of	Annotation,(
R11179	T16171	T16170	arg1Of	Annotation,Functional
R11180	T16171	T16172	arg1Of	Annotation,of
R11181	T16173	T16172	arg2Of	Mouse,of
R11182	T16174	T16169	arg3Of	),(
R11183	T16175	T16166	arg2Of	database,in
R11184	T16175	T16167	arg1Of	database,the
R11185	T16175	T16168	arg1Of	database,Fantom
R11186	T16178	T16176	arg1Of	primers,The
R11187	T16178	T16177	arg1Of	primers,PCR
R11188	T16178	T16179	arg1Of	primers,contained
R11189	T16180	T16181	arg1Of	XhoI,and
R11190	T16182	T16181	arg2Of	HindIII,and
R11191	T16183	T16179	arg2Of	sites,contained
R11192	T16183	T16180	arg1Of	sites,XhoI
R11193	T16183	T16182	arg1Of	sites,HindIII
R11194	T16183	T16184	arg1Of	sites,that
R11195	T16183	T16185	arg1Of	sites,were
R11196	T16183	T16186	arg2Of	sites,used
R11197	T16186	T16185	arg2Of	used,were
R11198	T16188	T16186	arg3Of	clone,used
R11199	T16188	T16187	arg1Of	clone,to
R11200	T16188	T16193	arg1Of	clone,upstream
R11201	T16192	T16188	arg2Of	fragment,clone
R11202	T16192	T16189	arg1Of	fragment,the
R11203	T16192	T16190	arg1Of	fragment,ɛy
R11204	T16192	T16191	arg1Of	fragment,promoter
R11205	T16193	T16194	arg1Of	upstream,of
R11206	T16198	T16194	arg2Of	gene,of
R11207	T16198	T16195	arg1Of	gene,the
R11208	T16198	T16196	arg1Of	gene,firefly
R11209	T16198	T16197	arg1Of	gene,luciferase
R11210	T16198	T16199	arg1Of	gene,in
R11211	T16201	T16200	arg1Of	Basic,pGL3
R11212	T16203	T16204	arg1Of	Promega,","
R11213	T16204	T16206	arg1Of	",",","
R11214	T16205	T16204	arg2Of	Madison,","
R11215	T16206	T16208	arg1Of	",",","
R11216	T16207	T16206	arg2Of	Wisconsin,","
R11217	T16208	T16199	arg2Of	",",in
R11218	T16208	T16201	arg1Of	",",Basic
R11219	T16208	T16202	arg2Of	",",(
R11220	T16210	T16208	arg2Of	States,","
R11221	T16210	T16209	arg1Of	States,United
R11222	T16211	T16202	arg3Of	),(
R11223	T16215	T16212	arg1Of	fragment,A
R11224	T16215	T16213	arg1Of	fragment,2.5-kb
R11225	T16215	T16214	arg1Of	fragment,SstI/XhoI
R11226	T16215	T16216	arg1Of	fragment,of
R11227	T16215	T16219	arg1Of	fragment,(
R11228	T16215	T16227	arg1Of	fragment,was
R11229	T16215	T16229	arg2Of	fragment,inserted
R11230	T16217	T16216	arg2Of	μLCRβ,of
R11231	T16217	T16218	arg1Of	μLCRβ,9.3
R11232	T16221	T16220	arg1Of	LCR,micro
R11233	T16221	T16222	arg1Of	LCR,;
R11234	T16222	T16219	arg2Of	;,(
R11235	T16224	T16222	arg2Of	31,;
R11236	T16224	T16223	arg2Of	31,[
R11237	T16225	T16223	arg3Of	],[
R11238	T16226	T16219	arg3Of	),(
R11239	T16229	T16227	arg2Of	inserted,was
R11240	T16229	T16228	arg1Of	inserted,then
R11241	T16229	T16230	arg1Of	inserted,upstream
R11242	T16229	T16235	arg1Of	inserted,in
R11243	T16229	T16241	arg1Of	inserted,","
R11244	T16229	T16242	modOf	inserted,resulting
R11245	T16230	T16231	arg1Of	upstream,of
R11246	T16234	T16231	arg2Of	promoter,of
R11247	T16234	T16232	arg1Of	promoter,the
R11248	T16234	T16233	arg1Of	promoter,ɛy
R11249	T16239	T16238	arg1Of	Basic,pGL3
R11250	T16240	T16235	arg2Of	plasmid,in
R11251	T16240	T16236	arg1Of	plasmid,the
R11252	T16240	T16237	arg1Of	plasmid,above
R11253	T16240	T16239	arg1Of	plasmid,Basic
R11254	T16242	T16243	arg1Of	resulting,in
R11255	T16246	T16243	arg2Of	construct,in
R11256	T16246	T16244	arg1Of	construct,a
R11257	T16246	T16245	arg1Of	construct,reporter
R11258	T16246	T16247	arg2Of	construct,in
R11259	T16246	T16248	arg1Of	construct,which
R11260	T16250	T16249	arg1Of	expression,luciferase
R11261	T16250	T16251	arg1Of	expression,is
R11262	T16250	T16252	arg2Of	expression,driven
R11263	T16252	T16247	arg1Of	driven,in
R11264	T16252	T16251	arg2Of	driven,is
R11265	T16256	T16252	arg1Of	promoter,driven
R11266	T16256	T16253	arg2Of	promoter,by
R11267	T16256	T16254	arg1Of	promoter,the
R11268	T16256	T16255	arg1Of	promoter,ɛy
R11269	T16258	T16257	arg1Of	series,A
R11270	T16258	T16259	arg1Of	series,of
R11271	T16258	T16266	arg1Of	series,were
R11272	T16258	T16267	arg2Of	series,generated
R11273	T16261	T16259	arg2Of	constructs,of
R11274	T16261	T16260	arg1Of	constructs,deletion
R11275	T16261	T16262	arg1Of	constructs,of
R11276	T16265	T16262	arg2Of	promoter,of
R11277	T16265	T16263	arg1Of	promoter,the
R11278	T16265	T16264	arg1Of	promoter,ɛy
R11279	T16267	T16266	arg2Of	generated,were
R11280	T16267	T16268	arg1Of	generated,similarly
R11281	T16270	T16269	arg1Of	primers,Forward
R11282	T16270	T16271	arg1Of	primers,with
R11283	T16270	T16275	arg1Of	primers,include
R11284	T16274	T16271	arg2Of	site,with
R11285	T16274	T16272	arg1Of	site,an
R11286	T16274	T16273	arg1Of	site,XhoI
R11287	T16275	T16276	arg1Of	include,:
R11288	T16277	T16275	arg2Of	MHB1457,include
R11289	T16277	T16278	arg1Of	MHB1457,","
R11290	T16277	T16285	arg1Of	MHB1457,;
R11291	T16279	T16278	arg2Of	5′CCGCTCGAGTGCTAGGCAAACACTCA3′,","
R11292	T16279	T16280	arg1Of	5′CCGCTCGAGTGCTAGGCAAACACTCA3′,(
R11293	T16283	T16280	arg2Of	−2052,(
R11294	T16283	T16281	arg1Of	−2052,−2077
R11295	T16283	T16282	arg1Of	−2052,to
R11296	T16284	T16280	arg3Of	),(
R11297	T16286	T16285	arg2Of	MHB1503,;
R11298	T16286	T16287	arg1Of	MHB1503,","
R11299	T16286	T16294	arg1Of	MHB1503,;
R11300	T16288	T16287	arg2Of	5′CCGCTCGAGTCTCTACACTGTCACTCCCTG3′,","
R11301	T16288	T16289	arg1Of	5′CCGCTCGAGTCTCTACACTGTCACTCCCTG3′,(
R11302	T16292	T16289	arg2Of	−605,(
R11303	T16292	T16290	arg1Of	−605,−634
R11304	T16292	T16291	arg1Of	−605,to
R11305	T16293	T16289	arg3Of	),(
R11306	T16295	T16294	arg2Of	MHB1505,;
R11307	T16295	T16296	arg1Of	MHB1505,","
R11308	T16295	T16303	arg1Of	MHB1505,;
R11309	T16297	T16296	arg2Of	5′CCGCTCGAGGGAGCCAAAAAAAGAATGC3′,","
R11310	T16297	T16298	arg1Of	5′CCGCTCGAGGGAGCCAAAAAAAGAATGC3′,(
R11311	T16301	T16298	arg2Of	−169,(
R11312	T16301	T16299	arg1Of	−169,−197
R11313	T16301	T16300	arg1Of	−169,to
R11314	T16302	T16298	arg3Of	),(
R11315	T16304	T16303	arg2Of	MHB1506,;
R11316	T16304	T16305	arg1Of	MHB1506,","
R11317	T16304	T16312	arg1Of	MHB1506,;
R11318	T16306	T16305	arg2Of	5′CCGCTCGAGCTGACCAATGGCTTCAAAG3′,","
R11319	T16306	T16307	arg1Of	5′CCGCTCGAGCTGACCAATGGCTTCAAAG3′,(
R11320	T16310	T16307	arg2Of	−58,(
R11321	T16310	T16308	arg1Of	−58,−85
R11322	T16310	T16309	arg1Of	−58,to
R11323	T16311	T16307	arg3Of	),(
R11324	T16313	T16314	arg1Of	MHB1532,","
R11325	T16313	T16322	arg1Of	MHB1532,and
R11326	T16315	T16314	arg2Of	5′CCGCTCGAGAATGCAGAACAAAGGGTCAGA3′,","
R11327	T16315	T16316	arg1Of	5′CCGCTCGAGAATGCAGAACAAAGGGTCAGA3′,(
R11328	T16319	T16316	arg2Of	−34,(
R11329	T16319	T16317	arg1Of	−34,−63
R11330	T16319	T16318	arg1Of	−34,to
R11331	T16320	T16316	arg3Of	),(
R11332	T16322	T16312	arg2Of	and,;
R11333	T16322	T16321	arg1Of	and,;
R11334	T16323	T16322	arg2Of	MHB1507,and
R11335	T16323	T16324	arg1Of	MHB1507,","
R11336	T16326	T16324	arg2Of	3′,","
R11337	T16326	T16325	arg1Of	3′,5′CCGCTCGAGGTCTGCGAAGAATAAAAGGC
R11338	T16326	T16327	arg1Of	3′,(
R11339	T16330	T16327	arg2Of	−9,(
R11340	T16330	T16328	arg1Of	−9,−37
R11341	T16330	T16329	arg1Of	−9,to
R11342	T16331	T16327	arg3Of	),(
R11343	T16334	T16332	arg1Of	primers,All
R11344	T16334	T16333	arg1Of	primers,forward
R11345	T16334	T16335	arg1Of	primers,were
R11346	T16334	T16336	arg2Of	primers,used
R11347	T16336	T16335	arg2Of	used,were
R11348	T16336	T16337	arg1Of	used,in
R11349	T16336	T16345	arg1Of	used,:
R11350	T16336	T16346	arg1Of	used,MHB1477
R11351	T16338	T16337	arg2Of	combination,in
R11352	T16338	T16339	arg1Of	combination,with
R11353	T16344	T16339	arg2Of	site,with
R11354	T16344	T16340	arg1Of	site,the
R11355	T16344	T16341	arg1Of	site,reverse
R11356	T16344	T16342	arg1Of	site,primer
R11357	T16344	T16343	arg1Of	site,HindIII
R11358	T16346	T16347	arg1Of	MHB1477,","
R11359	T16348	T16347	arg2Of	5′CGGAAGCTTGGGAGGTTGCTGGTGA3′,","
R11360	T16348	T16349	arg1Of	5′CGGAAGCTTGGGAGGTTGCTGGTGA3′,(
R11361	T16352	T16349	arg2Of	+20,(
R11362	T16352	T16350	arg1Of	+20,+45
R11363	T16352	T16351	arg1Of	+20,to
R11364	T16353	T16349	arg3Of	),(
R11365	T16354	T16355	arg1Of	Sox6-pcDNA3.1,[
R11366	T16354	T16358	arg1Of	Sox6-pcDNA3.1,was
R11367	T16354	T16359	arg2Of	Sox6-pcDNA3.1,used
R11368	T16354	T16361	arg1Of	Sox6-pcDNA3.1,overexpress
R11369	T16356	T16355	arg2Of	15,[
R11370	T16357	T16355	arg3Of	],[
R11371	T16359	T16358	arg2Of	used,was
R11372	T16361	T16359	arg3Of	overexpress,used
R11373	T16361	T16360	arg1Of	overexpress,to
R11374	T16362	T16361	arg2Of	Sox6,overexpress
R11375	T16365	T16363	arg1Of	version,A
R11376	T16365	T16364	arg2Of	version,truncated
R11377	T16365	T16366	arg1Of	version,of
R11378	T16365	T16379	arg1Of	version,was
R11379	T16365	T16380	arg2Of	version,generated
R11380	T16370	T16366	arg2Of	construct,of
R11381	T16370	T16367	arg1Of	construct,the
R11382	T16370	T16368	arg1Of	construct,Sox6
R11383	T16370	T16369	arg1Of	construct,overexpression
R11384	T16370	T16371	arg1Of	construct,(
R11385	T16370	T16374	arg1Of	construct,that
R11386	T16370	T16375	arg1Of	construct,lacks
R11387	T16372	T16371	arg2Of	Sox6-ΔHMG-pcDNA3.1,(
R11388	T16373	T16371	arg3Of	),(
R11389	T16378	T16375	arg2Of	domain,lacks
R11390	T16378	T16376	arg1Of	domain,the
R11391	T16378	T16377	arg1Of	domain,HMG
R11392	T16380	T16379	arg2Of	generated,was
R11393	T16380	T16381	arg1Of	generated,","
R11394	T16380	T16382	arg1Of	generated,as
R11395	T16383	T16382	arg2Of	described,as
R11396	T16385	T16383	arg1Of	others,described
R11397	T16385	T16384	arg2Of	others,by
R11398	T16385	T16386	arg1Of	others,[
R11399	T16387	T16386	arg2Of	32,[
R11400	T16388	T16386	arg3Of	],[
R11401	T16389	T16390	arg1Of	Mutagenesis,of
R11402	T16389	T16399	arg1Of	Mutagenesis,were
R11403	T16389	T16400	arg2Of	Mutagenesis,done
R11404	T16394	T16390	arg2Of	sites,of
R11405	T16394	T16391	arg1Of	sites,Sox/Sox6
R11406	T16394	T16392	arg1Of	sites,consensus
R11407	T16394	T16393	arg1Of	sites,binding
R11408	T16394	T16395	arg1Of	sites,of
R11409	T16398	T16395	arg2Of	promoter,of
R11410	T16398	T16396	arg1Of	promoter,the
R11411	T16398	T16397	arg1Of	promoter,ɛy
R11412	T16400	T16399	arg2Of	done,were
R11413	T16402	T16400	arg1Of	PCR,done
R11414	T16402	T16401	arg2Of	PCR,by
R11415	T16404	T16403	arg1Of	primers,Forward
R11416	T16404	T16405	arg2Of	primers,used
R11417	T16404	T16414	arg1Of	primers,include
R11418	T16407	T16405	arg3Of	generate,used
R11419	T16407	T16406	arg1Of	generate,to
R11420	T16413	T16407	arg2Of	constructs,generate
R11421	T16413	T16408	arg1Of	constructs,these
R11422	T16413	T16409	arg2Of	constructs,mutagenized
R11423	T16413	T16410	arg1Of	constructs,ɛy
R11424	T16413	T16411	arg1Of	constructs,promoter
R11425	T16413	T16412	arg1Of	constructs,reporter
R11426	T16414	T16415	arg1Of	include,:
R11427	T16416	T16414	arg2Of	MHB1661,include
R11428	T16416	T16417	arg1Of	MHB1661,","
R11429	T16416	T16424	arg1Of	MHB1661,;
R11430	T16418	T16417	arg2Of	5′CCGCTCGAGAATGCAGTGCCAAGGGTCAGAACATTGTCTGCGAAG3′,","
R11431	T16418	T16419	arg1Of	5′CCGCTCGAGAATGCAGTGCCAAGGGTCAGAACATTGTCTGCGAAG3′,(
R11432	T16422	T16419	arg2Of	−19,(
R11433	T16422	T16420	arg1Of	−19,−63
R11434	T16422	T16421	arg1Of	−19,to
R11435	T16423	T16419	arg3Of	),(
R11436	T16425	T16426	arg1Of	MHB1662,","
R11437	T16425	T16434	arg1Of	MHB1662,and
R11438	T16427	T16426	arg2Of	5′CCGCTCGAGAATGCAGAACAAAGGGTCAGATGAGTGTCTGCGAAGAA3′,","
R11439	T16427	T16428	arg1Of	5′CCGCTCGAGAATGCAGAACAAAGGGTCAGATGAGTGTCTGCGAAGAA3′,(
R11440	T16431	T16428	arg2Of	−16,(
R11441	T16431	T16429	arg1Of	−16,−63
R11442	T16431	T16430	arg1Of	−16,to
R11443	T16432	T16428	arg3Of	),(
R11444	T16434	T16424	arg2Of	and,;
R11445	T16434	T16433	arg1Of	and,;
R11446	T16435	T16434	arg2Of	MHB1663,and
R11447	T16435	T16436	arg1Of	MHB1663,","
R11448	T16438	T16436	arg2Of	3′,","
R11449	T16438	T16437	arg1Of	3′,5′CCGCTCGAGAATGCATGCCAAGGGTCAGATGAGTGTCTGCGAAGAA
R11450	T16438	T16439	arg1Of	3′,(
R11451	T16442	T16439	arg2Of	−18,(
R11452	T16442	T16440	arg1Of	−18,−63
R11453	T16442	T16441	arg1Of	−18,to
R11454	T16443	T16439	arg3Of	),(
R11937	T17191	T17192	arg1Of	Quantitation,of
R11938	T17194	T17192	arg2Of	mRNA,of
R11939	T17194	T17193	arg1Of	mRNA,globin
R11940	T17195	T17196	arg1Of	RNA,was
R11941	T17198	T17197	arg1Of	reverse,first
R11942	T17201	T17196	arg2Of	cDNA,was
R11943	T17201	T17198	arg1Of	cDNA,reverse
R11944	T17201	T17199	arg2Of	cDNA,transcribed
R11945	T17201	T17200	arg1Of	cDNA,to
R11946	T17202	T17203	arg1Of	Primers,for
R11947	T17202	T17210	arg1Of	Primers,were
R11948	T17202	T17211	arg2Of	Primers,obtained
R11949	T17206	T17203	arg2Of	amplification,for
R11950	T17206	T17204	arg1Of	amplification,cDNA
R11951	T17206	T17205	arg1Of	amplification,PCR
R11952	T17206	T17207	arg1Of	amplification,of
R11953	T17209	T17207	arg2Of	genes,of
R11954	T17209	T17208	arg1Of	genes,globin
R11955	T17211	T17210	arg2Of	obtained,were
R11956	T17211	T17212	arg1Of	obtained,from
R11957	T17213	T17212	arg2Of	Primerbank,from
R11958	T17213	T17214	arg1Of	Primerbank,[
R11959	T17215	T17214	arg2Of	51,[
R11960	T17216	T17214	arg3Of	],[
R11961	T17218	T17217	arg1Of	primers,All
R11962	T17218	T17219	arg1Of	primers,were
R11963	T17218	T17220	arg2Of	primers,searched
R11964	T17220	T17219	arg2Of	searched,were
R11965	T17220	T17221	arg1Of	searched,against
R11966	T17220	T17225	modOf	searched,to
R11967	T17224	T17221	arg2Of	database,against
R11968	T17224	T17222	arg1Of	database,the
R11969	T17224	T17223	arg1Of	database,NCBI
R11970	T17226	T17225	arg1Of	confirm,to
R11971	T17227	T17226	arg2Of	specificity,confirm
R11972	T17230	T17228	arg1Of	globin,For
R11973	T17230	T17229	arg1Of	globin,ɛy
R11974	T17230	T17231	arg1Of	globin,:
R11975	T17232	T17231	arg2Of	MHB1666,:
R11976	T17232	T17233	arg1Of	MHB1666,","
R11977	T17232	T17238	arg1Of	MHB1666,","
R11978	T17234	T17236	arg1Of	5′TGGCCTGTGGAGTAAGGTCAA3′,and
R11979	T17236	T17233	arg2Of	and,","
R11980	T17236	T17235	arg1Of	and,;
R11981	T17237	T17236	arg2Of	MHB1667,and
R11982	T17239	T17238	arg2Of	5′GAAGCAGAGGACAAGTTCCCA3′,","
R11983	T17242	T17240	arg1Of	globin,For
R11984	T17242	T17241	arg1Of	globin,ζ
R11985	T17242	T17243	arg1Of	globin,:
R11986	T17244	T17243	arg2Of	MHB1668,:
R11987	T17244	T17245	arg1Of	MHB1668,","
R11988	T17244	T17250	arg1Of	MHB1668,","
R11989	T17246	T17248	arg1Of	5′CTACCCCCAGACGAAGACCTA3′,and
R11990	T17248	T17245	arg2Of	and,","
R11991	T17248	T17247	arg1Of	and,;
R11992	T17249	T17248	arg2Of	MHB1669,and
R11993	T17251	T17250	arg2Of	5′CTTAACCGCATCCCCTACGG3′,","
R11994	T17254	T17252	arg1Of	globin,For
R11995	T17254	T17253	arg1Of	globin,βH1
R11996	T17254	T17255	arg1Of	globin,:
R11997	T17256	T17255	arg2Of	MHB1672,:
R11998	T17256	T17257	arg1Of	MHB1672,","
R11999	T17256	T17262	arg1Of	MHB1672,","
R12000	T17258	T17260	arg1Of	5′TGGACAACCTCAAGGAGACC3′,and
R12001	T17260	T17257	arg2Of	and,","
R12002	T17260	T17259	arg1Of	and,;
R12003	T17261	T17260	arg2Of	MHB1673,and
R12004	T17263	T17262	arg2Of	5′ACCTCTGGGGTGAATTCCTT3′,","
R12005	T17266	T17265	arg1Of	globin,βmaj/min
R12006	T17266	T17267	arg1Of	globin,:
R12007	T17267	T17264	arg1Of	:,For
R12008	T17267	T17269	arg1Of	:,:
R12009	T17268	T17267	arg2Of	MHB1674,:
R12010	T17270	T17272	arg1Of	5′ATGGCCTGAATCACTTGGAC3′,and
R12011	T17272	T17271	arg1Of	and,;
R12012	T17273	T17272	arg2Of	MHB1675,and
R12013	T17273	T17274	arg1Of	MHB1675,","
R12014	T17275	T17274	arg2Of	5′ACGATCATATTGCCCAGGAG3′,","
R12015	T17276	T17284	arg1Of	Using,(
R12016	T17281	T17276	arg2Of	kit,Using
R12017	T17281	T17277	arg1Of	kit,the
R12018	T17281	T17278	arg1Of	kit,SYBR
R12019	T17281	T17279	arg1Of	kit,green
R12020	T17281	T17280	arg1Of	kit,supermix
R12021	T17281	T17282	arg1Of	kit,with
R12022	T17283	T17282	arg2Of	ROX,with
R12023	T17285	T17286	arg1Of	Bio-Rad,","
R12024	T17286	T17288	arg1Of	",",","
R12025	T17287	T17286	arg2Of	Hercules,","
R12026	T17288	T17290	arg1Of	",",","
R12027	T17289	T17288	arg2Of	California,","
R12028	T17290	T17284	arg2Of	",",(
R12029	T17292	T17290	arg2Of	States,","
R12030	T17292	T17291	arg1Of	States,United
R12031	T17293	T17284	arg3Of	),(
R12032	T17296	T17295	arg1Of	amplification,PCR
R12033	T17296	T17297	arg1Of	amplification,was
R12034	T17296	T17298	arg2Of	amplification,run
R12035	T17298	T17276	modOf	run,Using
R12036	T17298	T17294	arg1Of	run,","
R12037	T17298	T17297	arg2Of	run,was
R12038	T17298	T17299	arg1Of	run,on
R12039	T17301	T17299	arg2Of	ABI7000,on
R12040	T17301	T17300	arg1Of	ABI7000,an
R12041	T17301	T17302	arg1Of	ABI7000,(
R12042	T17301	T17314	arg1Of	ABI7000,at
R12043	T17304	T17303	arg1Of	Biosystems,Applied
R12044	T17304	T17305	arg1Of	Biosystems,","
R12045	T17305	T17308	arg1Of	",",","
R12046	T17307	T17305	arg2Of	City,","
R12047	T17307	T17306	arg1Of	City,Foster
R12048	T17308	T17310	arg1Of	",",","
R12049	T17309	T17308	arg2Of	California,","
R12050	T17310	T17302	arg2Of	",",(
R12051	T17312	T17310	arg2Of	States,","
R12052	T17312	T17311	arg1Of	States,United
R12053	T17313	T17302	arg3Of	),(
R12054	T17316	T17317	arg1Of	University,of
R12055	T17318	T17317	arg2Of	Arizona,of
R12056	T17320	T17314	arg2Of	facility,at
R12057	T17320	T17315	arg1Of	facility,the
R12058	T17320	T17316	arg1Of	facility,University
R12059	T17320	T17319	arg1Of	facility,core
R12060	T17322	T17321	arg1Of	PCR,All
R12061	T17322	T17323	arg1Of	PCR,was
R12062	T17322	T17324	arg2Of	PCR,performed
R12063	T17324	T17323	arg2Of	performed,was
R12064	T17324	T17325	arg1Of	performed,in
R12065	T17328	T17325	arg2Of	reaction,in
R12066	T17328	T17326	arg1Of	reaction,a
R12067	T17328	T17327	arg1Of	reaction,25-μl
R12068	T17328	T17329	arg1Of	reaction,with
R12069	T17334	T17329	arg2Of	supermix,with
R12070	T17334	T17330	arg1Of	supermix,12.5
R12071	T17334	T17331	arg1Of	supermix,μl
R12072	T17334	T17332	arg1Of	supermix,SYBR
R12073	T17334	T17333	arg1Of	supermix,green
R12074	T17337	T17335	arg1Of	levels,GAPDH
R12075	T17337	T17336	arg1Of	levels,mRNA
R12076	T17337	T17338	arg1Of	levels,were
R12077	T17337	T17339	arg2Of	levels,used
R12078	T17339	T17338	arg2Of	used,were
R12079	T17339	T17340	arg1Of	used,as
R12080	T17341	T17340	arg2Of	control,as
R12081	T17341	T17342	arg1Of	control,for
R12082	T17344	T17342	arg2Of	RNA,for
R12083	T17344	T17343	arg1Of	RNA,input
R12084	T17347	T17345	arg1Of	analyses,Standard
R12085	T17347	T17346	arg1Of	analyses,curve
R12086	T17347	T17348	arg1Of	analyses,were
R12087	T17347	T17349	arg2Of	analyses,performed
R12088	T17349	T17348	arg2Of	performed,were
R12089	T17351	T17349	arg3Of	test,performed
R12090	T17351	T17350	arg1Of	test,to
R12091	T17353	T17351	arg2Of	efficiency,test
R12092	T17353	T17352	arg1Of	efficiency,the
R12093	T17353	T17354	arg1Of	efficiency,of
R12094	T17356	T17354	arg2Of	amplifications,of
R12095	T17356	T17355	arg1Of	amplifications,the
R12096	T17357	T17358	arg1Of	Triplicates,were
R12097	T17357	T17359	arg2Of	Triplicates,done
R12098	T17359	T17358	arg2Of	done,were
R12099	T17359	T17360	arg1Of	done,for
R12100	T17363	T17360	arg2Of	reaction,for
R12101	T17363	T17361	arg1Of	reaction,each
R12102	T17363	T17362	arg1Of	reaction,PCR
R12103	T17366	T17364	arg1Of	values,Relative
R12104	T17366	T17365	arg1Of	values,quantitative
R12105	T17366	T17367	arg1Of	values,were
R12106	T17366	T17368	arg2Of	values,calculated
R12107	T17366	T17381	arg1Of	values,normalized
R12108	T17368	T17367	arg2Of	calculated,were
R12109	T17368	T17369	arg1Of	calculated,in
R12110	T17368	T17380	arg1Of	calculated,and
R12111	T17375	T17369	arg2Of	Software,in
R12112	T17375	T17370	arg1Of	Software,the
R12113	T17375	T17371	arg1Of	Software,ABI
R12114	T17375	T17372	arg1Of	Software,Prism
R12115	T17375	T17373	arg1Of	Software,7000
R12116	T17375	T17374	arg1Of	Software,SDS
R12117	T17375	T17376	arg1Of	Software,(
R12118	T17378	T17376	arg2Of	Biosystems,(
R12119	T17378	T17377	arg1Of	Biosystems,Applied
R12120	T17379	T17376	arg3Of	),(
R12121	T17381	T17380	arg2Of	normalized,and
R12122	T17381	T17382	arg1Of	normalized,to
R12123	T17383	T17382	arg2Of	GAPDH,to
R12124	T17383	T17384	arg1Of	GAPDH,in
R12125	T17386	T17384	arg2Of	Excel,in
R12126	T17386	T17385	arg1Of	Excel,Microsoft
R12127	T17386	T17387	arg1Of	Excel,(
R12128	T17388	T17389	arg1Of	Redmond,","
R12129	T17389	T17391	arg1Of	",",","
R12130	T17390	T17389	arg2Of	Washington,","
R12131	T17392	T17391	arg2Of	United,","
R12132	T17393	T17387	arg2Of	States,(
R12133	T17393	T17388	arg1Of	States,Redmond
R12134	T17393	T17390	arg1Of	States,Washington
R12135	T17393	T17392	arg1Of	States,United
R12136	T17394	T17387	arg3Of	),(
R12381	T17767	T17766	arg1Of	situ,In
R12382	T17768	T17767	arg1Of	hybridization,situ
R12383	T17770	T17769	arg1Of	probes,Antisense
R12384	T17770	T17771	arg1Of	probes,were
R12385	T17770	T17772	arg2Of	probes,designed
R12386	T17772	T17771	arg2Of	designed,were
R12387	T17772	T17773	arg1Of	designed,to
R12388	T17777	T17774	arg1Of	nucleotides,murine
R12389	T17777	T17775	arg1Of	nucleotides,ɛy
R12390	T17777	T17776	arg1Of	nucleotides,globin
R12391	T17777	T17778	arg1Of	nucleotides,509–584
R12392	T17777	T17779	arg1Of	nucleotides,;
R12393	T17779	T17773	arg2Of	;,to
R12394	T17782	T17780	arg1Of	nucleotides,βmaj
R12395	T17782	T17781	arg1Of	nucleotides,globin
R12396	T17782	T17783	arg1Of	nucleotides,458–549
R12397	T17782	T17785	arg1Of	nucleotides,and
R12398	T17785	T17779	arg2Of	and,;
R12399	T17785	T17784	arg1Of	and,;
R12400	T17788	T17785	arg2Of	nucleotides,and
R12401	T17788	T17786	arg1Of	nucleotides,mouse
R12402	T17788	T17787	arg1Of	nucleotides,Sox6
R12403	T17788	T17789	arg1Of	nucleotides,1353–1927
R12404	T17790	T17791	arg1Of	Embryos,were
R12405	T17790	T17792	arg2Of	Embryos,fixed
R12406	T17790	T17811	arg1Of	Embryos,adhered
R12407	T17792	T17791	arg2Of	fixed,were
R12408	T17792	T17793	arg1Of	fixed,overnight
R12409	T17792	T17810	arg1Of	fixed,and
R12410	T17795	T17792	arg1Of	immersion,fixed
R12411	T17795	T17794	arg2Of	immersion,by
R12412	T17795	T17796	arg1Of	immersion,in
R12413	T17795	T17800	arg1Of	immersion,","
R12414	T17795	T17801	arg2Of	immersion,embedded
R12415	T17795	T17804	arg1Of	immersion,","
R12416	T17795	T17805	arg2Of	immersion,sectioned
R12417	T17797	T17798	arg1Of	4,%
R12418	T17799	T17796	arg2Of	paraformaldehyde,in
R12419	T17799	T17797	arg1Of	paraformaldehyde,4
R12420	T17801	T17802	arg1Of	embedded,in
R12421	T17803	T17802	arg2Of	paraffin,in
R12422	T17805	T17806	arg1Of	sectioned,at
R12423	T17808	T17806	arg2Of	μm,at
R12424	T17808	T17807	arg1Of	μm,5
R12425	T17810	T17809	arg1Of	and,","
R12426	T17811	T17810	arg2Of	adhered,and
R12427	T17813	T17811	arg2Of	charge,adhered
R12428	T17813	T17812	arg1Of	charge,to
R12429	T17815	T17813	arg2Of	slides,charge
R12430	T17815	T17814	arg2Of	slides,modified
R12431	T17815	T17816	arg1Of	slides,(
R12432	T17817	T17818	arg1Of	VWR,","
R12433	T17817	T17821	arg1Of	VWR,","
R12434	T17820	T17818	arg2Of	Chester,","
R12435	T17820	T17819	arg1Of	Chester,West
R12436	T17821	T17816	arg2Of	",",(
R12437	T17821	T17823	arg1Of	",",","
R12438	T17822	T17821	arg2Of	Pennsylvania,","
R12439	T17825	T17823	arg2Of	States,","
R12440	T17825	T17824	arg1Of	States,United
R12441	T17826	T17816	arg3Of	),(
R12442	T17827	T17828	arg1Of	Slides,were
R12443	T17827	T17829	arg2Of	Slides,processed
R12444	T17829	T17828	arg2Of	processed,were
R12445	T17829	T17830	arg1Of	processed,for
R12446	T17829	T17834	arg1Of	processed,as
R12447	T17832	T17831	arg1Of	situ,in
R12448	T17833	T17830	arg2Of	hybridization,for
R12449	T17833	T17832	arg1Of	hybridization,situ
R12450	T17835	T17834	arg2Of	described,as
R12451	T17835	T17836	arg1Of	described,[
R12452	T17835	T17839	modOf	described,using
R12453	T17837	T17836	arg2Of	52,[
R12454	T17838	T17836	arg3Of	],[
R12455	T17841	T17840	arg1Of	vitro,in
R12456	T17844	T17839	arg2Of	probes,using
R12457	T17844	T17841	arg1Of	probes,vitro
R12458	T17844	T17842	arg2Of	probes,transcribed
R12459	T17844	T17843	arg1Of	probes,RNA
R12460	T17844	T17845	arg2Of	probes,labeled
R12461	T17845	T17846	arg1Of	labeled,with
R12462	T17847	T17846	arg2Of	33P,with
R12463	T17848	T17849	arg1Of	Darkfield,and
R12464	T17850	T17849	arg2Of	brightfield,and
R12465	T17851	T17848	arg1Of	images,Darkfield
R12466	T17851	T17850	arg1Of	images,brightfield
R12467	T17851	T17852	arg1Of	images,were
R12468	T17851	T17853	arg2Of	images,obtained
R12469	T17853	T17852	arg2Of	obtained,were
R12470	T17853	T17854	arg1Of	obtained,with
R12471	T17857	T17856	arg1Of	Optiphot,Nikon
R12472	T17858	T17857	arg1Of	microscope,Optiphot
R12473	T17858	T17859	arg1Of	microscope,(
R12474	T17858	T17870	arg1Of	microscope,and
R12475	T17860	T17861	arg1Of	Nikon,","
R12476	T17861	T17863	arg1Of	",",","
R12477	T17862	T17861	arg2Of	Melville,","
R12478	T17863	T17866	arg1Of	",",","
R12479	T17865	T17863	arg2Of	York,","
R12480	T17865	T17864	arg1Of	York,New
R12481	T17866	T17859	arg2Of	",",(
R12482	T17868	T17866	arg2Of	States,","
R12483	T17868	T17867	arg1Of	States,United
R12484	T17869	T17859	arg3Of	),(
R12485	T17870	T17854	arg2Of	and,with
R12486	T17870	T17855	arg1Of	and,a
R12487	T17870	T17875	arg1Of	and,(
R12488	T17874	T17870	arg2Of	camera,and
R12489	T17874	T17871	arg1Of	camera,SPOT
R12490	T17874	T17872	arg1Of	camera,RT-Slider
R12491	T17874	T17873	arg1Of	camera,digital
R12492	T17877	T17878	arg1Of	Instruments,","
R12493	T17878	T17881	arg1Of	",",","
R12494	T17880	T17878	arg2Of	Heights,","
R12495	T17880	T17879	arg1Of	Heights,Sterling
R12496	T17881	T17876	arg1Of	",",Diagnostic
R12497	T17881	T17883	arg1Of	",",","
R12498	T17882	T17881	arg2Of	Michigan,","
R12499	T17883	T17875	arg2Of	",",(
R12500	T17885	T17883	arg2Of	States,","
R12501	T17885	T17884	arg1Of	States,United
R12502	T17886	T17875	arg3Of	),(
R12503	T17887	T17888	arg2Of	Objectives,used
R12504	T17887	T17889	arg1Of	Objectives,were
R12505	T17890	T17891	arg1Of	1×,(
R12506	T17890	T17896	arg1Of	1×,and
R12507	T17892	T17891	arg2Of	NA,(
R12508	T17892	T17893	arg1Of	NA,=
R12509	T17892	T17894	arg1Of	NA,0.04
R12510	T17895	T17891	arg3Of	),(
R12511	T17896	T17889	arg2Of	and,were
R12512	T17897	T17896	arg2Of	10×,and
R12513	T17897	T17898	arg1Of	10×,(
R12514	T17899	T17898	arg2Of	NA,(
R12515	T17899	T17900	arg1Of	NA,=
R12516	T17899	T17901	arg1Of	NA,0.5
R12517	T17902	T17898	arg3Of	),(
R12518	T17903	T17904	arg1Of	Images,were
R12519	T17903	T17905	arg2Of	Images,processed
R12520	T17903	T17907	arg2Of	Images,pseudocolored
R12521	T17903	T17910	arg2Of	Images,combined
R12522	T17905	T17906	arg1Of	processed,","
R12523	T17906	T17909	arg1Of	",",and
R12524	T17907	T17906	arg2Of	pseudocolored,","
R12525	T17909	T17904	arg2Of	and,were
R12526	T17909	T17908	arg1Of	and,","
R12527	T17909	T17940	arg2Of	and,plugins
R12528	T17910	T17909	arg2Of	combined,and
R12529	T17910	T17911	modOf	combined,using
R12530	T17912	T17911	arg2Of	Photoshop,using
R12531	T17912	T17913	arg1Of	Photoshop,(
R12532	T17914	T17915	arg1Of	Adobe,","
R12533	T17915	T17918	arg1Of	",",","
R12534	T17917	T17915	arg2Of	Jose,","
R12535	T17917	T17916	arg1Of	Jose,San
R12536	T17918	T17920	arg1Of	",",","
R12537	T17919	T17918	arg2Of	California,","
R12538	T17920	T17913	arg2Of	",",(
R12539	T17922	T17920	arg2Of	States,","
R12540	T17922	T17921	arg1Of	States,United
R12541	T17923	T17913	arg3Of	),(
R12542	T17924	T17925	arg1Of	software,with
R12543	T17924	T17940	arg1Of	software,plugins
R12544	T17927	T17925	arg2Of	Pro,with
R12545	T17927	T17926	arg1Of	Pro,Fovea
R12546	T17927	T17928	arg1Of	Pro,(
R12547	T17930	T17929	arg1Of	Graphics,Reindeer
R12548	T17930	T17931	arg1Of	Graphics,","
R12549	T17931	T17933	arg1Of	",",","
R12550	T17932	T17931	arg2Of	Asheville,","
R12551	T17933	T17936	arg1Of	",",","
R12552	T17935	T17933	arg2Of	Carolina,","
R12553	T17935	T17934	arg1Of	Carolina,North
R12554	T17936	T17928	arg2Of	",",(
R12555	T17938	T17936	arg2Of	States,","
R12556	T17938	T17937	arg1Of	States,United
R12557	T17939	T17928	arg3Of	),(
R12558	T17942	T17941	arg1Of	images,Original
R12559	T17942	T17943	arg1Of	images,are
R12560	T17942	T17944	arg1Of	images,available
R12561	T17944	T17943	arg2Of	available,are
R12754	T18187	T18186	arg1Of	embryos,18.5-dpc
R12755	T18187	T18188	arg1Of	embryos,were
R12756	T18187	T18189	arg2Of	embryos,exsanguinated
R12757	T18189	T18188	arg2Of	exsanguinated,were
R12758	T18189	T18190	arg1Of	exsanguinated,and
R12759	T18193	T18191	arg1Of	smears,peripheral
R12760	T18193	T18192	arg1Of	smears,blood
R12761	T18193	T18194	arg1Of	smears,were
R12762	T18193	T18195	arg2Of	smears,prepared
R12763	T18195	T18190	arg2Of	prepared,and
R12764	T18195	T18194	arg2Of	prepared,were
R12765	T18195	T18196	arg1Of	prepared,from
R12766	T18198	T18199	arg1Of	mutant,and
R12767	T18199	T18197	arg1Of	and,both
R12768	T18200	T18199	arg2Of	WT,and
R12769	T18201	T18196	arg2Of	mice,from
R12770	T18201	T18198	arg1Of	mice,mutant
R12771	T18201	T18200	arg1Of	mice,WT
R12772	T18203	T18202	arg1Of	slides,The
R12773	T18203	T18204	arg1Of	slides,were
R12774	T18203	T18205	arg2Of	slides,Wright-stained
R12775	T18203	T18207	arg2Of	slides,read
R12776	T18205	T18206	arg1Of	Wright-stained,and
R12777	T18206	T18204	arg2Of	and,were
R12778	T18207	T18206	arg2Of	read,and
R12779	T18209	T18207	arg1Of	DAF,read
R12780	T18209	T18208	arg2Of	DAF,by
R12781	T18213	T18210	arg2Of	analysis,For
R12782	T18213	T18211	arg1Of	analysis,whole
R12783	T18213	T18212	arg1Of	analysis,mount
R12784	T18215	T18216	arg1Of	14.5-dpc,WT
R12785	T18215	T18217	arg1Of	14.5-dpc,and
R12786	T18218	T18217	arg2Of	mutant,and
R12787	T18219	T18215	arg1Of	embryos,14.5-dpc
R12788	T18219	T18218	arg1Of	embryos,mutant
R12789	T18219	T18221	arg1Of	embryos,and
R12790	T18221	T18220	arg1Of	and,","
R12791	T18221	T18225	arg1Of	and,were
R12792	T18221	T18226	arg2Of	and,fixed
R12793	T18221	T18240	arg2Of	and,stained
R12794	T18224	T18221	arg2Of	mice,and
R12795	T18224	T18222	arg1Of	mice,postnatal
R12796	T18224	T18223	arg1Of	mice,day–10.5
R12797	T18226	T18227	arg1Of	fixed,in
R12798	T18226	T18239	arg1Of	fixed,and
R12799	T18228	T18229	arg1Of	10,%
R12800	T18230	T18227	arg2Of	formalin,in
R12801	T18230	T18228	arg1Of	formalin,10
R12802	T18230	T18231	arg1Of	formalin,","
R12803	T18230	T18233	arg1Of	formalin,","
R12804	T18230	T18234	arg2Of	formalin,sectioned
R12805	T18232	T18231	arg2Of	paraffin-embedded,","
R12806	T18234	T18235	arg1Of	sectioned,at
R12807	T18237	T18235	arg2Of	μm,at
R12808	T18237	T18236	arg1Of	μm,5
R12809	T18239	T18210	arg1Of	and,For
R12810	T18239	T18214	arg1Of	and,","
R12811	T18239	T18225	arg2Of	and,were
R12812	T18239	T18238	arg1Of	and,","
R12813	T18240	T18239	arg2Of	stained,and
R12814	T18240	T18241	arg1Of	stained,with
R12815	T18242	T18243	arg1Of	hematoxylin,and
R12816	T18243	T18241	arg2Of	and,with
R12817	T18244	T18243	arg2Of	eosin,and
R12818	T18246	T18245	arg1Of	samples,Liver
R12819	T18246	T18247	arg1Of	samples,(
R12820	T18246	T18255	arg1Of	samples,were
R12821	T18246	T18256	arg2Of	samples,prepared
R12822	T18248	T18247	arg2Of	at,(
R12823	T18250	T18249	arg1Of	dpc,14.5
R12824	T18250	T18251	arg1Of	dpc,and
R12825	T18251	T18248	arg2Of	and,at
R12826	T18253	T18251	arg2Of	dpc,and
R12827	T18253	T18252	arg1Of	dpc,18.5
R12828	T18254	T18247	arg3Of	),(
R12829	T18256	T18255	arg2Of	prepared,were
R12830	T18256	T18257	arg1Of	prepared,in
R12831	T18260	T18257	arg2Of	manner,in
R12832	T18260	T18258	arg1Of	manner,a
R12833	T18260	T18259	arg1Of	manner,similar
R12834	T18261	T18262	arg1Of	Images,were
R12835	T18261	T18263	arg2Of	Images,obtained
R12836	T18263	T18262	arg2Of	obtained,were
R12837	T18263	T18264	arg1Of	obtained,with
R12838	T18267	T18264	arg2Of	microscope,with
R12839	T18267	T18265	arg1Of	microscope,Nikon
R12840	T18267	T18266	arg1Of	microscope,Labophot-2
R12841	T18268	T18269	arg2Of	Objectives,used
R12842	T18268	T18270	arg1Of	Objectives,were
R12843	T18274	T18273	arg1Of	160/0.17,40/0.65
R12844	T18275	T18270	arg2Of	Nikon,were
R12845	T18275	T18271	arg1Of	Nikon,E
R12846	T18275	T18272	arg1Of	Nikon,Plan
R12847	T18275	T18274	arg1Of	Nikon,160/0.17
R12848	T18275	T18276	arg1Of	Nikon,(
R12849	T18275	T18280	arg1Of	Nikon,","
R12850	T18278	T18276	arg2Of	objective,(
R12851	T18278	T18277	arg1Of	objective,40×
R12852	T18279	T18276	arg3Of	),(
R12853	T18282	T18283	arg1Of	Plan,100/1.25
R12854	T18286	T18280	arg2Of	Nikon,","
R12855	T18286	T18281	arg1Of	Nikon,E
R12856	T18286	T18282	arg1Of	Nikon,Plan
R12857	T18286	T18284	arg1Of	Nikon,oil
R12858	T18286	T18285	arg1Of	Nikon,160/0.17
R12859	T18286	T18287	arg1Of	Nikon,(
R12860	T18289	T18287	arg2Of	objective,(
R12861	T18289	T18288	arg1Of	objective,100×
R12862	T18290	T18287	arg3Of	),(
R12863	T18292	T18291	arg1Of	camera,The
R12864	T18292	T18293	arg1Of	camera,was
R12865	T18296	T18293	arg2Of	Coolpix,was
R12866	T18296	T18294	arg1Of	Coolpix,a
R12867	T18296	T18295	arg1Of	Coolpix,Nikon
R12868	T18296	T18297	arg1Of	Coolpix,4300
R12869	T18299	T18298	arg1Of	images,Original
R12870	T18299	T18300	arg1Of	images,are
R12871	T18299	T18301	arg1Of	images,available
R12872	T18301	T18300	arg2Of	available,are
R13006	T18462	T18461	arg1Of	blot,Northern
R13007	T18469	T18463	arg1Of	filter,A
R13008	T18469	T18464	arg1Of	filter,mouse
R13009	T18469	T18465	arg1Of	filter,embryonic
R13010	T18469	T18466	arg1Of	filter,tissue
R13011	T18469	T18467	arg1Of	filter,Northern
R13012	T18469	T18468	arg1Of	filter,blot
R13013	T18469	T18470	arg1Of	filter,(
R13014	T18469	T18480	arg1Of	filter,was
R13015	T18469	T18481	arg2Of	filter,hybridized
R13016	T18471	T18470	arg2Of	Seegene,(
R13017	T18471	T18472	arg1Of	Seegene,","
R13018	T18471	T18476	arg1Of	Seegene,","
R13019	T18473	T18474	arg1Of	Rockville,","
R13020	T18474	T18472	arg2Of	",",","
R13021	T18475	T18474	arg2Of	Maryland,","
R13022	T18478	T18476	arg2Of	States,","
R13023	T18478	T18477	arg1Of	States,United
R13024	T18479	T18470	arg3Of	),(
R13025	T18481	T18480	arg2Of	hybridized,was
R13026	T18481	T18482	arg1Of	hybridized,with
R13027	T18481	T18500	arg1Of	hybridized,","
R13028	T18481	T18501	arg1Of	hybridized,by
R13029	T18485	T18482	arg2Of	probe,with
R13030	T18485	T18483	arg1Of	probe,a
R13031	T18485	T18484	arg1Of	probe,Sox6
R13032	T18485	T18486	arg2Of	probe,generated
R13033	T18485	T18494	arg2Of	probe,labeled
R13034	T18486	T18487	arg1Of	generated,by
R13035	T18486	T18493	arg1Of	generated,and
R13036	T18488	T18487	arg2Of	RT-PCR,by
R13037	T18488	T18489	arg1Of	RT-PCR,(
R13038	T18490	T18489	arg2Of	nucleotides,(
R13039	T18490	T18491	arg1Of	nucleotides,1353–1927
R13040	T18492	T18489	arg3Of	),(
R13041	T18494	T18493	arg2Of	labeled,and
R13042	T18494	T18495	arg1Of	labeled,with
R13043	T18497	T18496	arg2Of	α-32P,[
R13044	T18498	T18496	arg3Of	],[
R13045	T18499	T18495	arg2Of	dCTP,with
R13046	T18499	T18496	arg1Of	dCTP,[
R13047	T18504	T18501	arg2Of	labeling,by
R13048	T18504	T18502	arg1Of	labeling,random
R13049	T18504	T18503	arg1Of	labeling,primer
R13050	T18504	T18505	arg1Of	labeling,(
R13051	T18506	T18507	arg1Of	RediprimeII,;
R13052	T18507	T18505	arg2Of	;,(
R13053	T18507	T18514	arg1Of	;,","
R13054	T18509	T18508	arg1Of	Biosciences,Amersham
R13055	T18509	T18510	arg1Of	Biosciences,","
R13056	T18510	T18512	arg1Of	",",","
R13057	T18511	T18510	arg2Of	Buckinghamshire,","
R13058	T18512	T18507	arg2Of	",",;
R13059	T18513	T18512	arg2Of	England,","
R13060	T18516	T18514	arg2Of	Kingdom,","
R13061	T18516	T18515	arg1Of	Kingdom,United
R13062	T18517	T18505	arg3Of	),(
R13063	T18519	T18518	arg1Of	hybridization,The
R13064	T18519	T18520	arg1Of	hybridization,was
R13065	T18519	T18521	arg2Of	hybridization,performed
R13066	T18521	T18520	arg2Of	performed,was
R13067	T18521	T18522	arg1Of	performed,in
R13068	T18523	T18522	arg2Of	phosphate,in
R13069	T18523	T18524	arg2Of	phosphate,buffered
R13070	T18525	T18526	arg1Of	7,%
R13071	T18529	T18524	arg3Of	solution,buffered
R13072	T18529	T18525	arg1Of	solution,7
R13073	T18529	T18527	arg1Of	solution,SDS
R13074	T18529	T18528	arg1Of	solution,hybridization
R13075	T18530	T18531	arg1Of	Blots,were
R13076	T18530	T18532	arg2Of	Blots,washed
R13077	T18532	T18531	arg2Of	washed,were
R13078	T18532	T18533	arg1Of	washed,with
R13079	T18535	T18536	arg1Of	SSC,","
R13080	T18536	T18534	arg1Of	",",0.2×
R13081	T18538	T18536	arg2Of	%,","
R13082	T18538	T18537	arg1Of	%,1
R13083	T18539	T18533	arg2Of	SDS,with
R13084	T18539	T18535	arg1Of	SDS,SSC
R13085	T18539	T18538	arg1Of	SDS,%
R13086	T18539	T18540	arg1Of	SDS,at
R13087	T18539	T18543	arg1Of	SDS,prior
R13088	T18539	T18549	arg1Of	SDS,(
R13089	T18539	T18560	arg1Of	SDS,at
R13090	T18542	T18540	arg2Of	°C,at
R13091	T18542	T18541	arg1Of	°C,60
R13092	T18543	T18544	arg1Of	prior,to
R13093	T18545	T18544	arg2Of	exposure,to
R13094	T18545	T18546	arg1Of	exposure,to
R13095	T18548	T18546	arg2Of	film,to
R13096	T18548	T18547	arg1Of	film,X-ray
R13097	T18550	T18551	arg1Of	Kodak,","
R13098	T18551	T18553	arg1Of	",",","
R13099	T18552	T18551	arg2Of	Rochester,","
R13100	T18553	T18556	arg1Of	",",","
R13101	T18555	T18553	arg2Of	York,","
R13102	T18555	T18554	arg1Of	York,New
R13103	T18556	T18549	arg2Of	",",(
R13104	T18558	T18556	arg2Of	States,","
R13105	T18558	T18557	arg1Of	States,United
R13106	T18559	T18549	arg3Of	),(
R13107	T18562	T18560	arg2Of	°C,at
R13108	T18562	T18561	arg1Of	°C,−80
R13109	T18562	T18563	arg1Of	°C,for
R13110	T18565	T18563	arg2Of	d,for
R13111	T18565	T18564	arg1Of	d,6
R13227	T18724	T18725	arg1Of	culture,and
R13228	T18725	T18723	arg1Of	and,Cell
R13229	T18726	T18725	arg2Of	transfection,and
R13230	T18728	T18727	arg1Of	cells,GM979
R13231	T18728	T18729	arg1Of	cells,(
R13232	T18728	T18742	arg1Of	cells,were
R13233	T18728	T18743	arg2Of	cells,cultured
R13234	T18732	T18730	arg1Of	Repositories,Coriell
R13235	T18732	T18731	arg1Of	Repositories,Cell
R13236	T18732	T18733	arg1Of	Repositories,","
R13237	T18733	T18735	arg1Of	",",","
R13238	T18734	T18733	arg2Of	Camden,","
R13239	T18736	T18735	arg2Of	New,","
R13240	T18737	T18729	arg2Of	Jersey,(
R13241	T18737	T18732	arg1Of	Jersey,Repositories
R13242	T18737	T18734	arg1Of	Jersey,Camden
R13243	T18737	T18736	arg1Of	Jersey,New
R13244	T18737	T18738	arg1Of	Jersey,","
R13245	T18740	T18738	arg2Of	States,","
R13246	T18740	T18739	arg1Of	States,United
R13247	T18741	T18729	arg3Of	),(
R13248	T18743	T18742	arg2Of	cultured,were
R13249	T18743	T18744	arg1Of	cultured,in
R13250	T18745	T18746	arg2Of	Ham,'s
R13251	T18747	T18744	arg2Of	F12,in
R13252	T18747	T18746	arg1Of	F12,'s
R13253	T18747	T18748	arg1Of	F12,with
R13254	T18749	T18750	arg1Of	2,mM
R13255	T18751	T18748	arg2Of	L-glutamine,with
R13256	T18751	T18749	arg1Of	L-glutamine,2
R13257	T18751	T18752	arg2Of	L-glutamine,supplemented
R13258	T18752	T18753	arg1Of	supplemented,with
R13259	T18755	T18756	arg1Of	10,%
R13260	T18759	T18754	arg1Of	serum,heat-inactivated
R13261	T18759	T18755	arg1Of	serum,10
R13262	T18759	T18757	arg1Of	serum,fetal
R13263	T18759	T18758	arg1Of	serum,calf
R13264	T18759	T18760	arg1Of	serum,(
R13265	T18759	T18782	arg1Of	serum,and
R13266	T18761	T18762	arg1Of	Ivitrogen,","
R13267	T18762	T18764	arg1Of	",",","
R13268	T18763	T18762	arg2Of	Carlsbad,","
R13269	T18764	T18766	arg1Of	",",","
R13270	T18765	T18764	arg2Of	California,","
R13271	T18766	T18769	arg1Of	",",)
R13272	T18766	T18770	arg1Of	",",","
R13273	T18768	T18766	arg2Of	States,","
R13274	T18768	T18767	arg1Of	States,United
R13275	T18770	T18776	arg1Of	",",","
R13276	T18771	T18770	arg2Of	penicillin,","
R13277	T18771	T18772	arg1Of	penicillin,(
R13278	T18774	T18772	arg2Of	units/ml,(
R13279	T18774	T18773	arg1Of	units/ml,100
R13280	T18775	T18772	arg3Of	),(
R13281	T18776	T18760	arg2Of	",",(
R13282	T18779	T18776	arg2Of	μg/ml,","
R13283	T18779	T18777	arg1Of	μg/ml,streptomycin
R13284	T18779	T18778	arg1Of	μg/ml,100
R13285	T18780	T18760	arg3Of	),(
R13286	T18782	T18753	arg2Of	and,with
R13287	T18782	T18781	arg1Of	and,","
R13288	T18783	T18782	arg2Of	L-glutamine,and
R13289	T18783	T18784	arg1Of	L-glutamine,(
R13290	T18786	T18784	arg2Of	mM,(
R13291	T18786	T18785	arg1Of	mM,2
R13292	T18787	T18784	arg3Of	),(
R13293	T18789	T18788	arg1Of	cells,MEL
R13294	T18789	T18790	arg1Of	cells,were
R13295	T18789	T18791	arg2Of	cells,cultured
R13296	T18791	T18790	arg2Of	cultured,were
R13297	T18791	T18792	arg1Of	cultured,in
R13298	T18793	T18792	arg2Of	DMEM,in
R13299	T18793	T18794	arg2Of	DMEM,supplemented
R13300	T18794	T18795	arg1Of	supplemented,as
R13301	T18794	T18797	arg1Of	supplemented,(
R13302	T18796	T18795	arg2Of	above,as
R13303	T18798	T18797	arg2Of	without,(
R13304	T18799	T18798	arg2Of	heat,without
R13305	T18799	T18800	arg1Of	heat,inactivating
R13306	T18802	T18800	arg2Of	serum,inactivating
R13307	T18802	T18801	arg1Of	serum,the
R13308	T18803	T18797	arg3Of	),(
R13309	T18805	T18804	arg1Of	cells,GM979
R13310	T18805	T18806	arg1Of	cells,(
R13311	T18805	T18811	arg1Of	cells,in
R13312	T18805	T18816	arg1Of	cells,were
R13313	T18805	T18817	arg2Of	cells,transfected
R13314	T18808	T18806	arg2Of	×,(
R13315	T18808	T18807	arg1Of	×,4
R13316	T18808	T18809	arg1Of	×,105
R13317	T18810	T18806	arg3Of	),(
R13318	T18813	T18811	arg2Of	phase,in
R13319	T18813	T18812	arg1Of	phase,log
R13320	T18813	T18814	arg1Of	phase,of
R13321	T18815	T18814	arg2Of	growth,of
R13322	T18817	T18816	arg2Of	transfected,were
R13323	T18817	T18818	arg1Of	transfected,with
R13324	T18817	T18820	arg1Of	transfected,by
R13325	T18817	T18822	arg1Of	transfected,(
R13326	T18819	T18818	arg2Of	plasmids,with
R13327	T18821	T18820	arg2Of	FuGENE6,by
R13328	T18823	T18824	arg1Of	Roche,","
R13329	T18824	T18826	arg1Of	",",","
R13330	T18825	T18824	arg2Of	Indianapolis,","
R13331	T18826	T18828	arg1Of	",",","
R13332	T18827	T18826	arg2Of	Indiana,","
R13333	T18829	T18828	arg2Of	United,","
R13334	T18830	T18822	arg2Of	States,(
R13335	T18830	T18823	arg1Of	States,Roche
R13336	T18830	T18825	arg1Of	States,Indianapolis
R13337	T18830	T18827	arg1Of	States,Indiana
R13338	T18830	T18829	arg1Of	States,United
R13339	T18831	T18822	arg3Of	),(
R13340	T18832	T18833	arg1Of	Cells,were
R13341	T18832	T18834	arg2Of	Cells,transfected
R13342	T18834	T18833	arg2Of	transfected,were
R13343	T18834	T18835	arg1Of	transfected,with
R13344	T18834	T18844	arg1Of	transfected,along
R13345	T18839	T18835	arg2Of	constructs,with
R13346	T18839	T18836	arg1Of	constructs,ɛy
R13347	T18839	T18837	arg1Of	constructs,promoter
R13348	T18839	T18838	arg1Of	constructs,reporter
R13349	T18839	T18840	arg1Of	constructs,(
R13350	T18842	T18840	arg2Of	ng,(
R13351	T18842	T18841	arg1Of	ng,500
R13352	T18843	T18840	arg3Of	),(
R13353	T18844	T18845	arg1Of	along,with
R13354	T18848	T18847	arg1Of	vector,empty
R13355	T18848	T18849	arg1Of	vector,or
R13356	T18849	T18845	arg2Of	or,with
R13357	T18849	T18846	arg1Of	or,either
R13358	T18852	T18849	arg2Of	vector,or
R13359	T18852	T18850	arg1Of	vector,Sox6
R13360	T18852	T18851	arg1Of	vector,overexpression
R13361	T18852	T18853	arg1Of	vector,(
R13362	T18855	T18853	arg2Of	ng,(
R13363	T18855	T18854	arg1Of	ng,1000
R13364	T18856	T18853	arg3Of	),(
R13365	T18858	T18857	arg2Of	assays,In
R13366	T18858	T18859	arg1Of	assays,of
R13367	T18861	T18859	arg2Of	effect,of
R13368	T18861	T18860	arg1Of	effect,dosage
R13369	T18863	T18864	arg1Of	we,used
R13370	T18864	T18857	arg1Of	used,In
R13371	T18864	T18862	arg1Of	used,","
R13372	T18866	T18865	arg1Of	ng,200
R13373	T18866	T18867	arg1Of	ng,","
R13374	T18867	T18871	arg1Of	",",and
R13375	T18869	T18867	arg2Of	ng,","
R13376	T18869	T18868	arg1Of	ng,500
R13377	T18871	T18864	arg2Of	and,used
R13378	T18871	T18870	arg1Of	and,","
R13379	T18871	T18874	arg1Of	and,)
R13380	T18871	T18880	modOf	and,ega
R13381	T18873	T18871	arg2Of	ng,and
R13382	T18873	T18872	arg1Of	ng,1000
R13383	T18876	T18875	arg1Of	MV 1,. pRL-C
R13384	T18876	T18877	arg1Of	MV 1,5
R13385	T18876	T18880	arg1Of	MV 1,ega
R13386	T18876	T18881	arg2Of	MV 1,) wa
R13387	T18878	T18877	arg2Of	ng (Pro,5
R13388	T18879	T18877	arg3Of	m,5
R13389	T18881	T18880	arg2Of	) wa,ega
R13390	T18881	T18882	arg1Of	) wa,s
R13391	T18884	T18882	arg2Of	sed as ,s
R13392	T18884	T18883	arg1Of	sed as ,u
R13393	T18884	T18885	arg1Of	sed as ,a c
R13394	T18887	T18885	arg2Of	ransfectio,a c
R13395	T18887	T18886	arg1Of	ransfectio,ontrol for t
R13578	T19152	T19150	arg1Of	extract,Nuclear
R13579	T19152	T19151	arg1Of	extract,protein
R13580	T19152	T19153	arg1Of	extract,and
R13581	T19155	T19154	arg1Of	vitro,in
R13582	T19156	T19153	arg2Of	translation,and
R13583	T19156	T19155	arg1Of	translation,vitro
R13584	T19156	T19157	arg1Of	translation,of
R13585	T19158	T19157	arg2Of	Sox6,of
R13586	T19160	T19159	arg1Of	extracts,Nuclear
R13587	T19160	T19161	arg1Of	extracts,were
R13588	T19160	T19162	arg2Of	extracts,prepared
R13589	T19162	T19161	arg2Of	prepared,were
R13590	T19162	T19163	arg1Of	prepared,from
R13591	T19165	T19163	arg2Of	cells,from
R13592	T19165	T19164	arg1Of	cells,MEL
R13593	T19165	T19166	arg1Of	cells,(
R13594	T19165	T19171	arg1Of	cells,using
R13595	T19167	T19168	arg1Of	2,×
R13596	T19168	T19166	arg2Of	×,(
R13597	T19169	T19168	arg2Of	107,×
R13598	T19170	T19166	arg3Of	),(
R13599	T19173	T19171	arg2Of	kit,using
R13600	T19173	T19172	arg1Of	kit,a
R13601	T19173	T19174	arg1Of	kit,(
R13602	T19176	T19177	arg1Of	Motif,","
R13603	T19177	T19179	arg1Of	",",","
R13604	T19178	T19177	arg2Of	Carlsbad,","
R13605	T19179	T19175	arg1Of	",",Active
R13606	T19179	T19181	arg1Of	",",","
R13607	T19180	T19179	arg2Of	California,","
R13608	T19181	T19174	arg2Of	",",(
R13609	T19183	T19181	arg2Of	States,","
R13610	T19183	T19182	arg1Of	States,United
R13611	T19184	T19174	arg3Of	),(
R13612	T19188	T19187	arg1Of	vitro,in
R13613	T19191	T19185	arg1Of	vector,The
R13614	T19191	T19186	arg1Of	vector,Sox6
R13615	T19191	T19188	arg1Of	vector,vitro
R13616	T19191	T19189	arg1Of	vector,translation
R13617	T19191	T19190	arg1Of	vector,expression
R13618	T19191	T19192	arg1Of	vector,","
R13619	T19191	T19193	arg2Of	vector,tagged
R13620	T19191	T19199	arg1Of	vector,was
R13621	T19191	T19200	arg2Of	vector,described
R13622	T19193	T19194	arg1Of	tagged,with
R13623	T19195	T19196	arg1Of	c-Myc,and
R13624	T19196	T19194	arg2Of	and,with
R13625	T19197	T19196	arg2Of	HA,and
R13626	T19200	T19198	arg1Of	described,","
R13627	T19200	T19199	arg2Of	described,was
R13628	T19200	T19201	arg1Of	described,before
R13629	T19203	T19201	arg2Of	15,before
R13630	T19203	T19202	arg2Of	15,[
R13631	T19204	T19202	arg3Of	],[
R13632	T19206	T19205	arg1Of	translation,The
R13633	T19206	T19207	arg1Of	translation,was
R13634	T19206	T19208	arg2Of	translation,performed
R13635	T19208	T19207	arg2Of	performed,was
R13636	T19208	T19209	arg1Of	performed,in
R13637	T19212	T19209	arg2Of	lysate,in
R13638	T19212	T19210	arg1Of	lysate,a
R13639	T19212	T19211	arg1Of	lysate,reticulocyte
R13640	T19212	T19213	arg2Of	lysate,based
R13641	T19213	T19215	arg1Of	based,vitro
R13642	T19215	T19214	arg1Of	vitro,in
R13643	T19217	T19209	arg3Of	system,in
R13644	T19217	T19216	arg1Of	system,translational
R13645	T19217	T19218	arg1Of	system,(
R13646	T19223	T19218	arg2Of	Systems,(
R13647	T19223	T19219	arg1Of	Systems,TNT®
R13648	T19223	T19220	arg1Of	Systems,Quick
R13649	T19223	T19221	arg1Of	Systems,Coupled
R13650	T19223	T19222	arg1Of	Systems,Transcription/Translation
R13651	T19223	T19224	arg1Of	Systems,","
R13652	T19223	T19225	arg1Of	Systems,Promega
R13653	T19226	T19218	arg3Of	),(
R13654	T19228	T19227	arg1Of	vector,A
R13655	T19228	T19229	arg1Of	vector,without
R13656	T19228	T19234	arg1Of	vector,was
R13657	T19228	T19236	arg2Of	vector,translated
R13658	T19233	T19229	arg2Of	sequence,without
R13659	T19233	T19230	arg1Of	sequence,the
R13660	T19233	T19231	arg1Of	sequence,Sox6
R13661	T19233	T19232	arg1Of	sequence,coding
R13662	T19236	T19234	arg2Of	translated,was
R13663	T19236	T19235	arg1Of	translated,also
R13664	T19236	T19237	arg1Of	translated,as
R13665	T19240	T19237	arg2Of	control,as
R13666	T19240	T19238	arg1Of	control,a
R13667	T19240	T19239	arg1Of	control,negative
R13772	T19429	T19428	arg1Of	antibodies,Sox6
R13773	T19429	T19430	arg2Of	antibodies,used
R13774	T19429	T19434	arg1Of	antibodies,were
R13775	T19429	T19437	arg2Of	antibodies,provided
R13776	T19429	T19454	arg2Of	antibodies,obtained
R13777	T19430	T19431	arg1Of	used,in
R13778	T19433	T19431	arg2Of	study,in
R13779	T19433	T19432	arg1Of	study,this
R13780	T19437	T19436	arg1Of	provided,kindly
R13781	T19437	T19452	arg1Of	provided,or
R13782	T19441	T19437	arg1Of	Lalli,provided
R13783	T19441	T19438	arg2Of	Lalli,by
R13784	T19441	T19439	arg1Of	Lalli,Dr.
R13785	T19441	T19440	arg1Of	Lalli,Enzo
R13786	T19441	T19442	arg1Of	Lalli,(
R13787	T19445	T19443	arg1Of	Pasteur,Université
R13788	T19445	T19444	arg1Of	Pasteur,Louis
R13789	T19445	T19446	arg1Of	Pasteur,","
R13790	T19446	T19442	arg2Of	",",(
R13791	T19447	T19446	arg2Of	France,","
R13792	T19447	T19448	arg1Of	France,[
R13793	T19449	T19448	arg2Of	7,[
R13794	T19450	T19448	arg3Of	],[
R13795	T19451	T19442	arg3Of	),(
R13796	T19452	T19434	arg2Of	or,were
R13797	T19452	T19435	arg1Of	or,either
R13798	T19454	T19452	arg2Of	obtained,or
R13799	T19454	T19453	arg1Of	obtained,commercially
R13800	T19454	T19455	arg1Of	obtained,(
R13801	T19459	T19455	arg2Of	X,(
R13802	T19459	T19456	arg1Of	X,Catalog
R13803	T19459	T19457	arg1Of	X,No.
R13804	T19459	T19458	arg1Of	X,sc-17332
R13805	T19459	T19460	arg1Of	X,","
R13806	T19459	T19469	arg1Of	X,","
R13807	T19463	T19460	arg2Of	Biotechnology,","
R13808	T19463	T19461	arg1Of	Biotechnology,Santa
R13809	T19463	T19462	arg1Of	Biotechnology,Cruz
R13810	T19463	T19464	arg1Of	Biotechnology,","
R13811	T19466	T19465	arg1Of	Cruz,Santa
R13812	T19466	T19467	arg1Of	Cruz,","
R13813	T19467	T19464	arg2Of	",",","
R13814	T19468	T19467	arg2Of	California,","
R13815	T19471	T19469	arg2Of	States,","
R13816	T19471	T19470	arg1Of	States,United
R13817	T19472	T19455	arg3Of	),(
R13818	T19474	T19473	arg1Of	Sox6,All
R13819	T19474	T19481	arg1Of	Sox6,was
R13820	T19474	T19482	arg2Of	Sox6,purchased
R13821	T19475	T19476	arg1Of	antibodies,generated
R13822	T19480	T19476	arg2Of	antibody,generated
R13823	T19480	T19477	arg1Of	antibody,similar
R13824	T19480	T19478	arg1Of	antibody,results.
R13825	T19480	T19479	arg1Of	antibody,c-Myc
R13826	T19482	T19476	arg3Of	purchased,generated
R13827	T19482	T19481	arg2Of	purchased,was
R13828	T19482	T19483	arg1Of	purchased,from
R13829	T19485	T19483	arg2Of	Normal,from
R13830	T19485	T19484	arg1Of	Normal,Invitrogen.
R13831	T19488	T19486	arg1Of	antibody,rabbit
R13832	T19488	T19487	arg1Of	antibody,IgG
R13833	T19488	T19489	arg1Of	antibody,was
R13834	T19488	T19490	arg2Of	antibody,obtained
R13835	T19490	T19481	modOf	obtained,was
R13836	T19490	T19489	arg2Of	obtained,was
R13837	T19490	T19491	arg1Of	obtained,from
R13838	T19493	T19491	arg2Of	Biotechnology,from
R13839	T19493	T19492	arg1Of	Biotechnology,Upstate
R13840	T19493	T19494	arg1Of	Biotechnology,(
R13841	T19496	T19494	arg2Of	Placid,(
R13842	T19496	T19495	arg1Of	Placid,Lake
R13843	T19496	T19497	arg1Of	Placid,","
R13844	T19496	T19500	arg1Of	Placid,","
R13845	T19499	T19497	arg2Of	York,","
R13846	T19499	T19498	arg1Of	York,New
R13847	T19502	T19500	arg2Of	States,","
R13848	T19502	T19501	arg1Of	States,United
R13849	T19503	T19494	arg3Of	),(
R13935	T19697	T19695	arg1Of	oligonucleotides,Single-stranded
R13936	T19697	T19696	arg1Of	oligonucleotides,complementary
R13937	T19697	T19698	arg1Of	oligonucleotides,were
R13938	T19697	T19699	arg2Of	oligonucleotides,annealed
R13939	T19697	T19701	arg2Of	oligonucleotides,end-labeled
R13940	T19699	T19700	arg1Of	annealed,and
R13941	T19700	T19698	arg2Of	and,were
R13942	T19701	T19700	arg2Of	end-labeled,and
R13943	T19701	T19702	arg1Of	end-labeled,with
R13944	T19704	T19703	arg2Of	γ-32P,[
R13945	T19705	T19703	arg3Of	],[
R13946	T19706	T19702	arg2Of	ATP,with
R13947	T19706	T19703	arg1Of	ATP,[
R13948	T19706	T19707	arg1Of	ATP,with
R13949	T19710	T19707	arg2Of	kinase,with
R13950	T19710	T19708	arg1Of	kinase,T4
R13951	T19710	T19709	arg1Of	kinase,polynucleotide
R13952	T19711	T19712	arg1Of	EMSA,was
R13953	T19711	T19713	arg2Of	EMSA,performed
R13954	T19713	T19712	arg2Of	performed,was
R13955	T19713	T19714	arg1Of	performed,with
R13956	T19713	T19738	arg1Of	performed,:
R13957	T19716	T19715	arg1Of	μg,5
R13958	T19716	T19717	arg1Of	μg,of
R13959	T19716	T19723	arg1Of	μg,or
R13960	T19719	T19717	arg2Of	proteins,of
R13961	T19719	T19718	arg1Of	proteins,nuclear
R13962	T19719	T19720	arg1Of	proteins,from
R13963	T19722	T19720	arg2Of	cells,from
R13964	T19722	T19721	arg1Of	cells,MEL
R13965	T19723	T19714	arg2Of	or,with
R13966	T19723	T19731	arg1Of	or,with
R13967	T19725	T19723	arg2Of	μl,or
R13968	T19725	T19724	arg1Of	μl,3
R13969	T19725	T19726	arg1Of	μl,of
R13970	T19728	T19727	arg1Of	vitro-translated,in
R13971	T19729	T19726	arg2Of	Sox6,of
R13972	T19729	T19728	arg1Of	Sox6,vitro-translated
R13973	T19731	T19730	arg1Of	with,along
R13974	T19734	T19731	arg2Of	lysate,with
R13975	T19734	T19732	arg1Of	lysate,the
R13976	T19734	T19733	arg1Of	lysate,reticulocyte
R13977	T19734	T19735	arg1Of	lysate,in
R13978	T19737	T19735	arg2Of	buffer,in
R13979	T19737	T19736	arg1Of	buffer,binding
R13980	T19739	T19740	arg1Of	100,mM
R13981	T19741	T19739	arg1Of	NaCl,100
R13982	T19741	T19742	arg1Of	NaCl,","
R13983	T19742	T19746	arg1Of	",",","
R13984	T19743	T19744	arg1Of	10,%
R13985	T19745	T19742	arg2Of	glycerol,","
R13986	T19745	T19743	arg1Of	glycerol,10
R13987	T19746	T19750	arg1Of	",",","
R13988	T19749	T19746	arg2Of	BSA,","
R13989	T19749	T19747	arg1Of	BSA,200
R13990	T19749	T19748	arg1Of	BSA,ng/μl
R13991	T19750	T19757	arg1Of	",",or
R13992	T19753	T19750	arg2Of	poly,","
R13993	T19753	T19751	arg1Of	poly,50
R13994	T19753	T19752	arg1Of	poly,ng/μl
R13995	T19753	T19754	arg1Of	poly,(
R13996	T19755	T19754	arg2Of	dI-dC,(
R13997	T19756	T19754	arg3Of	),(
R13998	T19757	T19770	arg1Of	or,","
R13999	T19758	T19759	arg1Of	poly,(
R14000	T19758	T19762	arg1Of	poly,","
R14001	T19760	T19759	arg2Of	dG-dC,(
R14002	T19761	T19759	arg3Of	),(
R14003	T19762	T19757	arg2Of	",",or
R14004	T19763	T19764	arg1Of	10,mM
R14005	T19765	T19762	arg2Of	HEPES,","
R14006	T19765	T19763	arg1Of	HEPES,10
R14007	T19765	T19766	arg1Of	HEPES,(
R14008	T19767	T19766	arg2Of	pH,(
R14009	T19767	T19768	arg1Of	pH,7
R14010	T19769	T19766	arg3Of	),(
R14011	T19771	T19772	arg1Of	0.1,mM
R14012	T19773	T19770	arg2Of	EDTA,","
R14013	T19773	T19771	arg1Of	EDTA,0.1
R14014	T19773	T19774	arg1Of	EDTA,","
R14015	T19773	T19778	arg1Of	EDTA,","
R14016	T19775	T19776	arg1Of	0.25,mM
R14017	T19777	T19774	arg2Of	DTT,","
R14018	T19777	T19775	arg1Of	DTT,0.25
R14019	T19779	T19780	arg1Of	0.6,mM
R14020	T19781	T19778	arg2Of	MgCl2,","
R14021	T19781	T19779	arg1Of	MgCl2,0.6
R14022	T19783	T19784	arg1Of	competition,or
R14023	T19784	T19782	arg2Of	or,For
R14024	T19786	T19784	arg2Of	assays,or
R14025	T19786	T19785	arg1Of	assays,supershift
R14026	T19792	T19789	arg2Of	competitor,indicated
R14027	T19792	T19790	arg1Of	competitor,unlabelled
R14028	T19792	T19791	arg1Of	competitor,oligonucleotide
R14029	T19792	T19793	arg1Of	competitor,(
R14030	T19792	T19798	arg1Of	competitor,or
R14031	T19796	T19793	arg2Of	excess,(
R14032	T19796	T19794	arg1Of	excess,200-fold
R14033	T19796	T19795	arg1Of	excess,molar
R14034	T19797	T19793	arg3Of	),(
R14035	T19798	T19788	arg1Of	or,the
R14036	T19798	T19804	arg1Of	or,was
R14037	T19798	T19805	arg2Of	or,added
R14038	T19799	T19798	arg2Of	antibody,or
R14039	T19799	T19800	arg1Of	antibody,(
R14040	T19802	T19800	arg2Of	μl,(
R14041	T19802	T19801	arg1Of	μl,3
R14042	T19803	T19800	arg3Of	),(
R14043	T19805	T19782	arg1Of	added,For
R14044	T19805	T19787	arg1Of	added,","
R14045	T19805	T19804	arg2Of	added,was
R14046	T19806	T19808	arg1Of	30,to
R14047	T19808	T19807	arg1Of	to,min
R14048	T19809	T19808	arg2Of	60,to
R14049	T19810	T19805	arg3Of	min,added
R14050	T19810	T19806	arg1Of	min,30
R14051	T19810	T19811	arg1Of	min,prior
R14052	T19811	T19812	arg1Of	prior,to
R14053	T19813	T19812	arg2Of	addition,to
R14054	T19813	T19814	arg1Of	addition,of
R14055	T19816	T19814	arg2Of	probe,of
R14056	T19816	T19815	arg2Of	probe,radiolabeled
R14057	T19818	T19817	arg2Of	addition,Following
R14058	T19818	T19819	arg1Of	addition,of
R14059	T19822	T19819	arg2Of	probe,of
R14060	T19822	T19820	arg1Of	probe,the
R14061	T19822	T19821	arg2Of	probe,radiolabeled
R14062	T19825	T19824	arg1Of	samples,the
R14063	T19825	T19826	arg1Of	samples,were
R14064	T19825	T19827	arg2Of	samples,incubated
R14065	T19825	T19838	arg2Of	samples,loaded
R14066	T19827	T19828	arg1Of	incubated,for
R14067	T19827	T19837	arg1Of	incubated,and
R14068	T19830	T19829	arg1Of	min,30
R14069	T19830	T19831	arg1Of	min,or
R14070	T19831	T19828	arg2Of	or,for
R14071	T19831	T19834	arg1Of	or,at
R14072	T19833	T19831	arg2Of	min,or
R14073	T19833	T19832	arg1Of	min,60
R14074	T19836	T19834	arg2Of	temperature,at
R14075	T19836	T19835	arg1Of	temperature,room
R14076	T19837	T19817	arg1Of	and,Following
R14077	T19837	T19823	arg1Of	and,","
R14078	T19837	T19826	arg2Of	and,were
R14079	T19838	T19837	arg2Of	loaded,and
R14080	T19838	T19839	arg1Of	loaded,on
R14081	T19842	T19843	arg1Of	%,or
R14082	T19843	T19841	arg1Of	or,4
R14083	T19844	T19843	arg2Of	6,or
R14084	T19845	T19839	arg2Of	%,on
R14085	T19845	T19840	arg1Of	%,a
R14086	T19845	T19842	arg1Of	%,%
R14087	T19845	T19844	arg1Of	%,6
R14088	T19847	T19846	arg2Of	w/v,(
R14089	T19848	T19846	arg3Of	),(
R14090	T19850	T19838	arg3Of	gel,loaded
R14091	T19850	T19846	arg1Of	gel,(
R14092	T19850	T19849	arg1Of	gel,polyacrylamide
R14093	T19851	T19852	arg1Of	Electrophoresis,was
R14094	T19851	T19853	arg2Of	Electrophoresis,performed
R14095	T19853	T19852	arg2Of	performed,was
R14096	T19853	T19854	arg1Of	performed,at
R14097	T19853	T19859	arg1Of	performed,for
R14098	T19853	T19862	arg1Of	performed,at
R14099	T19853	T19866	arg1Of	performed,and
R14100	T19858	T19854	arg2Of	mAmp,at
R14101	T19858	T19855	arg1Of	mAmp,a
R14102	T19858	T19856	arg1Of	mAmp,constant
R14103	T19858	T19857	arg1Of	mAmp,19
R14104	T19861	T19859	arg2Of	h,for
R14105	T19861	T19860	arg1Of	h,4–8
R14106	T19864	T19862	arg2Of	temperature,at
R14107	T19864	T19863	arg1Of	temperature,room
R14108	T19866	T19865	arg1Of	and,","
R14109	T19868	T19867	arg1Of	gels,the
R14110	T19868	T19869	arg1Of	gels,were
R14111	T19868	T19870	arg2Of	gels,dried
R14112	T19870	T19866	arg2Of	dried,and
R14113	T19870	T19869	arg2Of	dried,were
R14114	T19870	T19871	arg1Of	dried,prior
R14115	T19871	T19872	arg1Of	prior,to
R14116	T19873	T19872	arg2Of	autoradiography,to
R14117	T19874	T19875	arg2Of	Antibodies,used
R14118	T19874	T19879	arg1Of	Antibodies,included
R14119	T19875	T19876	arg1Of	used,for
R14120	T19878	T19876	arg2Of	analyses,for
R14121	T19878	T19877	arg1Of	analyses,supershift
R14122	T19880	T19881	arg1Of	c-Myc,","
R14123	T19881	T19884	arg1Of	",",and
R14124	T19882	T19881	arg2Of	HA,","
R14125	T19884	T19883	arg1Of	and,","
R14126	T19885	T19884	arg2Of	Sox6,and
R14127	T19886	T19879	arg2Of	antibodies,included
R14128	T19886	T19880	arg1Of	antibodies,c-Myc
R14129	T19886	T19882	arg1Of	antibodies,HA
R14130	T19886	T19885	arg1Of	antibodies,Sox6
R14131	T19886	T19887	arg1Of	antibodies,(
R14132	T19888	T19887	arg2Of	described,(
R14133	T19888	T19889	arg1Of	described,as
R14134	T19890	T19889	arg2Of	above,as
R14135	T19891	T19887	arg3Of	),(
R14136	T19894	T19892	arg1Of	sequences,The
R14137	T19894	T19893	arg1Of	sequences,DNA
R14138	T19894	T19895	arg1Of	sequences,of
R14139	T19894	T19898	arg1Of	sequences,are
R14140	T19894	T19899	arg1Of	sequences,as
R14141	T19897	T19895	arg2Of	oligonucleotides,of
R14142	T19897	T19896	arg1Of	oligonucleotides,the
R14143	T19898	T19901	arg1Of	are,(
R14144	T19898	T19908	arg1Of	are,:
R14145	T19899	T19898	arg2Of	as,are
R14146	T19900	T19899	arg2Of	follows,as
R14147	T19904	T19902	arg1Of	oligos,only
R14148	T19904	T19903	arg1Of	oligos,forward
R14149	T19904	T19905	arg1Of	oligos,are
R14150	T19904	T19906	arg2Of	oligos,listed
R14151	T19906	T19901	arg2Of	listed,(
R14152	T19906	T19905	arg2Of	listed,are
R14153	T19907	T19901	arg3Of	),(
R14154	T19913	T19909	arg2Of	probe,For
R14155	T19913	T19910	arg1Of	probe,the
R14156	T19913	T19911	arg1Of	probe,36-bp
R14157	T19913	T19912	arg1Of	probe,WT
R14158	T19913	T19914	arg1Of	probe,:
R14159	T19915	T19916	arg1Of	5′AATGCAGAACAAAGGGTCAGAACATTGTCTGCGAAG3′,(
R14160	T19915	T19919	arg1Of	5′AATGCAGAACAAAGGGTCAGAACATTGTCTGCGAAG3′,;
R14161	T19915	T19920	arg1Of	5′AATGCAGAACAAAGGGTCAGAACATTGTCTGCGAAG3′,for
R14162	T19915	T19927	arg1Of	5′AATGCAGAACAAAGGGTCAGAACATTGTCTGCGAAG3′,:
R14163	T19917	T19916	arg2Of	MHB1556,(
R14164	T19918	T19916	arg3Of	),(
R14165	T19922	T19920	arg2Of	probe,for
R14166	T19922	T19921	arg1Of	probe,mutant
R14167	T19922	T19923	arg1Of	probe,1
R14168	T19922	T19924	arg1Of	probe,(
R14169	T19925	T19924	arg2Of	M1,(
R14170	T19926	T19924	arg3Of	),(
R14171	T19928	T19929	arg1Of	5′AACAAAGGGTCAGAACATTGTCTGCGAAG3′,(
R14172	T19928	T19932	arg1Of	5′AACAAAGGGTCAGAACATTGTCTGCGAAG3′,;
R14173	T19928	T19933	arg1Of	5′AACAAAGGGTCAGAACATTGTCTGCGAAG3′,for
R14174	T19928	T19940	arg1Of	5′AACAAAGGGTCAGAACATTGTCTGCGAAG3′,:
R14175	T19930	T19929	arg2Of	MHB1644,(
R14176	T19931	T19929	arg3Of	),(
R14177	T19935	T19933	arg2Of	probe,for
R14178	T19935	T19934	arg1Of	probe,mutant
R14179	T19935	T19936	arg1Of	probe,2
R14180	T19935	T19937	arg1Of	probe,(
R14181	T19938	T19937	arg2Of	M2,(
R14182	T19939	T19937	arg3Of	),(
R14183	T19941	T19942	arg1Of	5′AATGCAGAACAAAGGGTCAGAtgagTGTCTGCGAAG3′,(
R14184	T19941	T19945	arg1Of	5′AATGCAGAACAAAGGGTCAGAtgagTGTCTGCGAAG3′,;
R14185	T19941	T19946	arg1Of	5′AATGCAGAACAAAGGGTCAGAtgagTGTCTGCGAAG3′,for
R14186	T19941	T19953	arg1Of	5′AATGCAGAACAAAGGGTCAGAtgagTGTCTGCGAAG3′,:
R14187	T19943	T19942	arg2Of	MHB1648,(
R14188	T19944	T19942	arg3Of	),(
R14189	T19948	T19946	arg2Of	probe,for
R14190	T19948	T19947	arg1Of	probe,mutant
R14191	T19948	T19949	arg1Of	probe,3
R14192	T19948	T19950	arg1Of	probe,(
R14193	T19951	T19950	arg2Of	M3,(
R14194	T19952	T19950	arg3Of	),(
R14195	T19954	T19955	arg1Of	5′AATGCAGtgccAAGGGTCAGAACATTGTCTGCGAAG3′,(
R14196	T19956	T19955	arg2Of	MHB1650,(
R14197	T19957	T19955	arg3Of	),(
R14488	T20383	T20382	arg1Of	assay,ChIP
R14489	T20385	T20384	arg2Of	described,As
R14490	T20387	T20385	arg1Of	Nouzova,described
R14491	T20387	T20386	arg2Of	Nouzova,by
R14492	T20387	T20388	arg1Of	Nouzova,[
R14493	T20387	T20391	arg1Of	Nouzova,","
R14494	T20387	T20392	arg1Of	Nouzova,in
R14495	T20387	T20394	arg1Of	Nouzova,:
R14496	T20389	T20388	arg2Of	53,[
R14497	T20390	T20388	arg3Of	],[
R14498	T20393	T20392	arg2Of	brief,in
R14499	T20395	T20396	arg1Of	Cells,from
R14500	T20395	T20413	arg1Of	Cells,were
R14501	T20395	T20414	arg2Of	Cells,treated
R14502	T20398	T20397	arg1Of	cells,MEL
R14503	T20398	T20399	arg1Of	cells,(
R14504	T20398	T20404	arg1Of	cells,or
R14505	T20401	T20399	arg2Of	×,(
R14506	T20401	T20400	arg1Of	×,4
R14507	T20401	T20402	arg1Of	×,107
R14508	T20403	T20399	arg3Of	),(
R14509	T20404	T20396	arg2Of	or,from
R14510	T20407	T20404	arg2Of	cells,or
R14511	T20407	T20405	arg1Of	cells,fetal
R14512	T20407	T20406	arg1Of	cells,liver
R14513	T20407	T20408	arg1Of	cells,from
R14514	T20412	T20408	arg2Of	mice,from
R14515	T20412	T20409	arg1Of	mice,three
R14516	T20412	T20410	arg1Of	mice,15.5-dpc
R14517	T20412	T20411	arg1Of	mice,WT
R14518	T20414	T20413	arg2Of	treated,were
R14519	T20414	T20415	arg1Of	treated,with
R14520	T20416	T20417	arg1Of	1,%
R14521	T20418	T20415	arg2Of	formaldehyde,with
R14522	T20418	T20416	arg1Of	formaldehyde,1
R14523	T20418	T20419	arg1Of	formaldehyde,for
R14524	T20421	T20419	arg2Of	min,for
R14525	T20421	T20420	arg1Of	min,10
R14526	T20421	T20422	arg1Of	min,at
R14527	T20421	T20425	arg1Of	min,","
R14528	T20421	T20426	arg2Of	min,rinsed
R14529	T20421	T20435	arg1Of	min,with
R14530	T20424	T20422	arg2Of	°C,at
R14531	T20424	T20423	arg1Of	°C,37
R14532	T20426	T20427	arg1Of	rinsed,in
R14533	T20430	T20431	arg2Of	Hanks,'
R14534	T20434	T20427	arg2Of	solution,in
R14535	T20434	T20428	arg1Of	solution,ice-cold
R14536	T20434	T20429	arg1Of	solution,1×
R14537	T20434	T20431	arg1Of	solution,'
R14538	T20434	T20432	arg1Of	solution,balanced
R14539	T20434	T20433	arg1Of	solution,salt
R14540	T20436	T20437	arg1Of	0.1,%
R14541	T20438	T20435	arg2Of	EDTA,with
R14542	T20438	T20436	arg1Of	EDTA,0.1
R14543	T20438	T20439	arg1Of	EDTA,containing
R14544	T20441	T20439	arg2Of	inhibitors,containing
R14545	T20441	T20440	arg1Of	inhibitors,protease
R14546	T20441	T20442	arg1Of	inhibitors,","
R14547	T20441	T20443	arg2Of	inhibitors,collected
R14548	T20441	T20450	arg2Of	inhibitors,resuspended
R14549	T20441	T20461	arg2Of	inhibitors,incubated
R14550	T20445	T20443	arg1Of	centrifugation,collected
R14551	T20445	T20444	arg2Of	centrifugation,by
R14552	T20445	T20446	arg1Of	centrifugation,at
R14553	T20448	T20446	arg2Of	°C,at
R14554	T20448	T20447	arg1Of	°C,4
R14555	T20450	T20451	arg1Of	resuspended,in
R14556	T20450	T20460	arg1Of	resuspended,and
R14557	T20455	T20451	arg2Of	buffer,in
R14558	T20455	T20452	arg1Of	buffer,a
R14559	T20455	T20453	arg1Of	buffer,SDS
R14560	T20455	T20454	arg1Of	buffer,lysis
R14561	T20455	T20456	arg1Of	buffer,containing
R14562	T20458	T20456	arg2Of	inhibitors,containing
R14563	T20458	T20457	arg1Of	inhibitors,protease
R14564	T20460	T20449	arg1Of	and,","
R14565	T20460	T20459	arg1Of	and,","
R14566	T20461	T20460	arg2Of	incubated,and
R14567	T20461	T20462	arg1Of	incubated,on
R14568	T20461	T20464	arg1Of	incubated,for
R14569	T20463	T20462	arg2Of	ice,on
R14570	T20466	T20464	arg2Of	min,for
R14571	T20466	T20465	arg1Of	min,10
R14572	T20468	T20467	arg1Of	complexes,DNA-protein
R14573	T20468	T20469	arg1Of	complexes,were
R14574	T20468	T20470	arg2Of	complexes,sonicated
R14575	T20470	T20469	arg2Of	sonicated,were
R14576	T20470	T20471	arg1Of	sonicated,to
R14577	T20472	T20473	arg1Of	200,and
R14578	T20474	T20473	arg2Of	600,and
R14579	T20475	T20471	arg2Of	bp,to
R14580	T20475	T20472	arg1Of	bp,200
R14581	T20475	T20474	arg1Of	bp,600
R14582	T20476	T20477	arg1Of	One-tenth,of
R14583	T20476	T20480	arg1Of	One-tenth,was
R14584	T20476	T20481	arg2Of	One-tenth,set
R14585	T20479	T20477	arg2Of	sample,of
R14586	T20479	T20478	arg1Of	sample,the
R14587	T20481	T20480	arg2Of	set,was
R14588	T20481	T20482	arg1Of	set,aside
R14589	T20481	T20483	arg1Of	set,for
R14590	T20481	T20487	arg1Of	set,and
R14591	T20485	T20483	arg2Of	control,for
R14592	T20485	T20484	arg1Of	control,input
R14593	T20487	T20486	arg1Of	and,","
R14594	T20487	T20496	arg1Of	and,(
R14595	T20490	T20488	arg1Of	sample,the
R14596	T20490	T20489	arg1Of	sample,remaining
R14597	T20490	T20491	arg1Of	sample,was
R14598	T20490	T20492	arg2Of	sample,precleared
R14599	T20492	T20487	arg2Of	precleared,and
R14600	T20492	T20491	arg2Of	precleared,was
R14601	T20492	T20493	arg1Of	precleared,with
R14602	T20495	T20493	arg2Of	A-Sepharose,with
R14603	T20495	T20494	arg1Of	A-Sepharose,protein
R14604	T20498	T20497	arg1Of	Biosciences,Amersham
R14605	T20498	T20499	arg1Of	Biosciences,","
R14606	T20499	T20501	arg1Of	",",","
R14607	T20500	T20499	arg2Of	Piscataway,","
R14608	T20501	T20504	arg1Of	",",","
R14609	T20503	T20501	arg2Of	Jersey,","
R14610	T20503	T20502	arg1Of	Jersey,New
R14611	T20504	T20496	arg2Of	",",(
R14612	T20506	T20504	arg2Of	States,","
R14613	T20506	T20505	arg1Of	States,United
R14614	T20507	T20496	arg3Of	),(
R14615	T20509	T20508	arg2Of	preclearing,Following
R14616	T20512	T20511	arg1Of	samples,the
R14617	T20512	T20513	arg1Of	samples,were
R14618	T20512	T20514	arg2Of	samples,split
R14619	T20514	T20513	arg2Of	split,were
R14620	T20514	T20515	arg1Of	split,into
R14621	T20514	T20532	arg1Of	split,and
R14622	T20516	T20515	arg2Of	thirds,into
R14623	T20516	T20517	arg1Of	thirds,:
R14624	T20519	T20517	arg2Of	sample,:
R14625	T20519	T20518	arg1Of	sample,one
R14626	T20519	T20520	arg2Of	sample,treated
R14627	T20520	T20521	arg1Of	treated,with
R14628	T20522	T20521	arg2Of	anti-Sox6,with
R14629	T20522	T20523	arg1Of	anti-Sox6,","
R14630	T20525	T20523	arg2Of	second,","
R14631	T20525	T20524	arg1Of	second,a
R14632	T20525	T20526	arg2Of	second,treated
R14633	T20526	T20527	arg1Of	treated,with
R14634	T20530	T20527	arg2Of	IgG,with
R14635	T20530	T20528	arg1Of	IgG,normal
R14636	T20530	T20529	arg1Of	IgG,rabbit
R14637	T20532	T20508	arg1Of	and,Following
R14638	T20532	T20510	arg1Of	and,","
R14639	T20532	T20531	arg1Of	and,","
R14640	T20535	T20533	arg1Of	sample,the
R14641	T20535	T20534	arg1Of	sample,third
R14642	T20535	T20536	arg1Of	sample,without
R14643	T20535	T20541	arg1Of	sample,were
R14644	T20535	T20542	arg2Of	sample,used
R14645	T20540	T20536	arg2Of	two,without
R14646	T20540	T20537	arg1Of	two,Ab.
R14647	T20540	T20538	arg1Of	two,The
R14648	T20540	T20539	arg1Of	two,last
R14649	T20542	T20532	arg2Of	used,and
R14650	T20542	T20541	arg2Of	used,were
R14651	T20542	T20543	arg1Of	used,as
R14652	T20545	T20543	arg2Of	controls,as
R14653	T20545	T20544	arg1Of	controls,negative
R14654	T20548	T20546	arg1Of	complexes,The
R14655	T20548	T20547	arg1Of	complexes,chromatin-antibody
R14656	T20548	T20549	arg1Of	complexes,were
R14657	T20548	T20550	arg2Of	complexes,eluted
R14658	T20550	T20549	arg2Of	eluted,were
R14659	T20550	T20552	arg1Of	eluted,and
R14660	T20552	T20551	arg1Of	and,","
R14661	T20556	T20553	arg1Of	cross-links,the
R14662	T20556	T20554	arg1Of	cross-links,DNA
R14663	T20556	T20555	arg1Of	cross-links,protein
R14664	T20556	T20557	arg1Of	cross-links,were
R14665	T20556	T20558	arg2Of	cross-links,reversed
R14666	T20558	T20552	arg2Of	reversed,and
R14667	T20558	T20557	arg2Of	reversed,were
R14668	T20558	T20559	arg1Of	reversed,with
R14669	T20558	T20563	arg1Of	reversed,at
R14670	T20560	T20561	arg1Of	5,M
R14671	T20562	T20559	arg2Of	NaCl,with
R14672	T20562	T20560	arg1Of	NaCl,5
R14673	T20565	T20563	arg2Of	°C,at
R14674	T20565	T20564	arg1Of	°C,65
R14675	T20565	T20566	arg1Of	°C,for
R14676	T20568	T20566	arg2Of	h,for
R14677	T20568	T20567	arg1Of	h,4
R14678	T20570	T20569	arg1Of	DNA,Input
R14679	T20570	T20571	arg1Of	DNA,or
R14680	T20571	T20574	arg1Of	or,was
R14681	T20571	T20575	arg2Of	or,used
R14682	T20573	T20571	arg2Of	DNA,or
R14683	T20573	T20572	arg2Of	DNA,immunoprecipitated
R14684	T20575	T20574	arg2Of	used,was
R14685	T20575	T20576	arg1Of	used,as
R14686	T20578	T20576	arg2Of	template,as
R14687	T20578	T20577	arg1Of	template,a
R14688	T20578	T20579	arg1Of	template,in
R14689	T20582	T20579	arg2Of	reaction,in
R14690	T20582	T20580	arg1Of	reaction,the
R14691	T20582	T20581	arg1Of	reaction,PCR
R14692	T20584	T20583	arg1Of	amplification,PCR
R14693	T20584	T20585	arg1Of	amplification,of
R14694	T20584	T20589	arg1Of	amplification,was
R14695	T20584	T20590	arg2Of	amplification,performed
R14696	T20584	T20592	arg1Of	amplification,yielded
R14697	T20588	T20585	arg2Of	promoter,of
R14698	T20588	T20586	arg1Of	promoter,the
R14699	T20588	T20587	arg1Of	promoter,ɛy
R14700	T20590	T20589	arg2Of	performed,was
R14701	T20590	T20591	arg1Of	performed,and
R14702	T20591	T20596	arg1Of	and,","
R14703	T20591	T20597	arg1Of	and,corresponding
R14704	T20592	T20591	arg2Of	yielded,and
R14705	T20595	T20592	arg2Of	amplicon,yielded
R14706	T20595	T20593	arg1Of	amplicon,a
R14707	T20595	T20594	arg1Of	amplicon,172-bp
R14708	T20598	T20597	arg2Of	to,corresponding
R14709	T20599	T20598	arg2Of	nucleotides,to
R14710	T20599	T20600	arg1Of	nucleotides,−31
R14711	T20600	T20601	arg1Of	−31,to
R14712	T20602	T20601	arg2Of	+140,to
R14713	T20602	T20603	arg1Of	+140,of
R14714	T20602	T20607	arg1Of	+140,(
R14715	T20606	T20603	arg2Of	promoter,of
R14716	T20606	T20604	arg1Of	promoter,the
R14717	T20606	T20605	arg1Of	promoter,ɛy
R14718	T20609	T20610	arg1Of	MHB1688,","
R14719	T20610	T20613	arg1Of	",",and
R14720	T20611	T20610	arg2Of	5′CGAAGAATAAAAGGCCACCA3′,","
R14721	T20613	T20607	arg2Of	and,(
R14722	T20613	T20608	arg1Of	and,primers
R14723	T20613	T20612	arg1Of	and,;
R14724	T20613	T20615	arg1Of	and,","
R14725	T20614	T20613	arg2Of	MHB1689,and
R14726	T20616	T20615	arg2Of	5′GCTTCACCACCAACCTCTTC3′,","
R14727	T20617	T20607	arg3Of	),(
R14728	T20618	T20619	arg1Of	PCR,was
R14729	T20618	T20620	arg2Of	PCR,performed
R14730	T20620	T20619	arg2Of	performed,was
R14731	T20620	T20621	arg1Of	performed,under
R14732	T20620	T20625	arg1Of	performed,:
R14733	T20624	T20621	arg2Of	conditions,under
R14734	T20624	T20622	arg1Of	conditions,the
R14735	T20624	T20623	arg1Of	conditions,following
R14736	T20627	T20626	arg1Of	°C,95
R14737	T20627	T20628	arg1Of	°C,for
R14738	T20627	T20631	arg1Of	°C,followed
R14739	T20627	T20655	arg1Of	°C,ending
R14740	T20630	T20628	arg2Of	min,for
R14741	T20630	T20629	arg1Of	min,15
R14742	T20631	T20632	arg1Of	followed,by
R14743	T20631	T20635	arg1Of	followed,at
R14744	T20631	T20638	arg1Of	followed,for
R14745	T20631	T20641	arg1Of	followed,","
R14746	T20631	T20651	arg1Of	followed,for
R14747	T20631	T20654	arg1Of	followed,","
R14748	T20631	T20655	modOf	followed,ending
R14749	T20634	T20632	arg2Of	cycles,by
R14750	T20634	T20633	arg1Of	cycles,30
R14751	T20637	T20635	arg2Of	°C,at
R14752	T20637	T20636	arg1Of	°C,95
R14753	T20640	T20638	arg2Of	s,for
R14754	T20640	T20639	arg1Of	s,30
R14755	T20643	T20642	arg1Of	°C,60
R14756	T20643	T20644	arg1Of	°C,for
R14757	T20643	T20648	arg1Of	°C,and
R14758	T20646	T20644	arg2Of	s,for
R14759	T20646	T20645	arg1Of	s,30
R14760	T20648	T20631	arg2Of	and,followed
R14761	T20648	T20647	arg1Of	and,","
R14762	T20650	T20648	arg2Of	°C,and
R14763	T20650	T20649	arg1Of	°C,72
R14764	T20653	T20651	arg2Of	s,for
R14765	T20653	T20652	arg1Of	s,45
R14766	T20655	T20656	arg1Of	ending,with
R14767	T20655	T20663	arg1Of	ending,for
R14768	T20659	T20656	arg2Of	extension,with
R14769	T20659	T20657	arg1Of	extension,a
R14770	T20659	T20658	arg1Of	extension,final
R14771	T20659	T20660	arg1Of	extension,at
R14772	T20662	T20660	arg2Of	°C,at
R14773	T20662	T20661	arg1Of	°C,72
R14774	T20665	T20663	arg2Of	min,for
R14775	T20665	T20664	arg1Of	min,5

bionlp-st-ge-2016-test-proteins

Id	Subject	Object	Predicate	Lexical cue
T20360	10215-10217	Protein	denotes	ɛy
T20359	10110-10112	Protein	denotes	ɛy
T20358	9756-9760	Protein	denotes	Sox6
T20357	9585-9594	Protein	denotes	protein A
T19138	7072-7076	Protein	denotes	Sox6
T19137	6864-6866	Protein	denotes	HA
T19136	6854-6859	Protein	denotes	c-Myc
T19135	6797-6801	Protein	denotes	Sox6
T19134	6666-6670	Protein	denotes	Sox6
T17176	3471-3476	Protein	denotes	GAPDH
T17175	3168-3173	Protein	denotes	GAPDH
T17174	2770-2780	Protein	denotes	min globin
T17173	2765-2769	Protein	denotes	βmaj
T17172	2675-2685	Protein	denotes	βH1 globin
T17171	2586-2594	Protein	denotes	ζ globin
T17170	2495-2504	Protein	denotes	ɛy globin
T16053	1906-1908	Protein	denotes	ɛy
T16052	1761-1765	Protein	denotes	Sox6
T16051	1730-1734	Protein	denotes	Sox6
T16050	1697-1701	Protein	denotes	Sox6
T16049	1654-1658	Protein	denotes	Sox6
T16048	1086-1088	Protein	denotes	ɛy
T16047	1034-1036	Protein	denotes	ɛy
T16046	995-1005	Protein	denotes	luciferase
T16045	907-909	Protein	denotes	ɛy
T16044	738-748	Protein	denotes	luciferase
T16043	693-695	Protein	denotes	ɛy
T16042	404-412	Protein	denotes	ɛ globin
T16041	294-295	Protein	denotes	ɛ
T16040	193-202	Protein	denotes	ɛy globin
T16039	137-139	Protein	denotes	ɛy
T16038	49-51	Protein	denotes	ɛy
T18458	5348-5352	Protein	denotes	Sox6
T17759	3673-3677	Protein	denotes	Sox6
T17758	3630-3641	Protein	denotes	βmaj globin
T17757	3599-3608	Protein	denotes	ɛy globin
T19674	8575-8579	Protein	denotes	Sox6
T19673	8567-8569	Protein	denotes	HA
T19672	8560-8565	Protein	denotes	c-Myc
T19671	7908-7911	Protein	denotes	BSA
T19670	7812-7816	Protein	denotes	Sox6
T19669	7691-7715	Protein	denotes	T4 polynucleotide kinase
T19410	7432-7437	Protein	denotes	c-Myc
T19409	7389-7393	Protein	denotes	Sox6
T19408	7149-7153	Protein	denotes	Sox6
T18716	6435-6439	Protein	denotes	Sox6
T18715	6360-6362	Protein	denotes	ɛy

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T19959	8575-8579	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T19958	7812-7816	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T19505	7389-7393	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T19504	7149-7153	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T19243	7072-7076	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T19242	6797-6801	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T19241	6666-6670	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T18888	6435-6439	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T17945	3673-3677	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T18566	5348-5352	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T17396	3471-3476	http://www.uniprot.org/uniprot/P04406	denotes	GAPDH
T17395	3168-3173	http://www.uniprot.org/uniprot/P04406	denotes	GAPDH
T16450	1870-1874	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T16449	1761-1765	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T16448	1730-1734	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T16447	1697-1701	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T16446	1654-1658	http://www.uniprot.org/uniprot/P35712	denotes	Sox6
T16445	995-1005	http://www.uniprot.org/uniprot/P08659	denotes	luciferase
T16444	738-748	http://www.uniprot.org/uniprot/P08659	denotes	luciferase

UBERON-AE

Id	Subject	Object	Predicate	Lexical cue
T20340	10475-10484	http://purl.obolibrary.org/obo/UBERON_2000106	denotes	extension
T20339	9097-9102	http://purl.obolibrary.org/obo/UBERON_0002107	denotes	liver
T18703	6190-6195	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T18702	5973-5978	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T18451	5252-5258	http://purl.obolibrary.org/obo/UBERON_0000479	denotes	tissue
T18450	5242-5258	http://purl.obolibrary.org/obo/UBERON_0005291	denotes	embryonic tissue
T18175	4601-4606	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood
T18174	4755-4762	http://purl.obolibrary.org/obo/UBERON_0000922	denotes	embryos
T18173	4559-4566	http://purl.obolibrary.org/obo/UBERON_0000922	denotes	embryos
T17742	4160-4167	http://purl.obolibrary.org/obo/UBERON_0002544	denotes	digital

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T19662	7801-7811	http://purl.obolibrary.org/obo/GO_0006412	denotes	translated
T17753	4150-4152	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T17752	3635-3641	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17751	3602-3608	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T20352	9349-9354	http://purl.obolibrary.org/obo/GO_0019835	denotes	lysis
T19128	7006-7019	http://purl.obolibrary.org/obo/GO_0006351	denotes	Transcription
T19127	7102-7112	http://purl.obolibrary.org/obo/GO_0006412	denotes	translated
T19126	6899-6910	http://purl.obolibrary.org/obo/GO_0006412	denotes	translation
T19125	6811-6822	http://purl.obolibrary.org/obo/GO_0006412	denotes	translation
T19124	6651-6662	http://purl.obolibrary.org/obo/GO_0006412	denotes	translation
T18710	6236-6242	http://purl.obolibrary.org/obo/GO_0040007	denotes	growth
T18456	5372-5374	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T17162	2774-2780	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17161	2679-2685	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17160	2588-2594	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17159	2498-2504	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17158	2366-2372	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17157	2272-2278	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T16013	503-518	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcriptional
T16012	473-486	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T16011	296-310	http://purl.obolibrary.org/obo/GO_0016458	denotes	gene silencing
T16010	406-412	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T16009	196-202	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T17182	2774-2780	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17181	2679-2685	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17180	2588-2594	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17179	2498-2504	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17178	2366-2372	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17177	2272-2278	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T16056	1885-1892	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T16055	406-412	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T16054	196-202	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T20361	9895-9903	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T19677	8580-8590	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T19676	8129-8137	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T19675	7855-7862	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T18459	5372-5374	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T17762	4150-4152	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T17761	3635-3641	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T17760	3602-3608	http://purl.obolibrary.org/obo/GO_0005344	denotes	globin
T19414	7496-7504	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T19413	7438-7446	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T19412	7394-7404	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T19411	7154-7164	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T20363	9103-9108	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T20362	9072-9077	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T19682	8580-8590	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T19681	8129-8137	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T19680	8580-8590	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T19679	8129-8137	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T19678	7775-7780	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T17183	3077-3081	http://purl.obolibrary.org/obo/GO_0019013	denotes	core
T20370	9895-9903	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T20369	9895-9903	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T20368	9885-9894	http://purl.obolibrary.org/obo/GO_0000785	denotes	chromatin
T20367	9431-9448	http://purl.obolibrary.org/obo/GO_0043234	denotes	protein complexes
T20366	9427-9448	http://purl.obolibrary.org/obo/GO_0097522	denotes	DNA-protein complexes
T20365	9427-9448	http://purl.obolibrary.org/obo/GO_0032993	denotes	DNA-protein complexes
T20364	9427-9448	http://purl.obolibrary.org/obo/GO_0001114	denotes	DNA-protein complexes
T19139	6712-6717	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T19423	7492-7504	http://purl.obolibrary.org/obo/GO_0071736	denotes	IgG antibody
T19422	7496-7504	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T19421	7438-7446	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T19420	7394-7404	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T19419	7154-7164	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T19418	7496-7504	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T19417	7438-7446	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T19416	7394-7404	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T19415	7154-7164	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T18720	6204-6209	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18719	6109-6114	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18718	5820-5824	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T18717	5768-5772	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell

sentences

Id	Subject	Object	Predicate	Lexical cue
T20351	10361-10504	Sentence	denotes	95 °C for 15 min followed by 30 cycles at 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 45 s, ending with a final extension at 72 °C for 5 min.
T20350	10311-10360	Sentence	denotes	PCR was performed under the following conditions:
T20349	10085-10310	Sentence	denotes	PCR amplification of the ɛy promoter was performed and yielded a 172-bp amplicon, corresponding to nucleotides −31 to +140 of the ɛy promoter (primers MHB1688, 5′CGAAGAATAAAAGGCCACCA3′; and MHB1689, 5′GCTTCACCACCAACCTCTTC3′).
T20348	10005-10084	Sentence	denotes	Input DNA or immunoprecipitated DNA was used as a template in the PCR reaction.
T20347	9881-10004	Sentence	denotes	The chromatin-antibody complexes were eluted, and the DNA protein cross-links were reversed with 5 M NaCl at 65 °C for 4 h.
T20346	9668-9880	Sentence	denotes	Following preclearing, the samples were split into thirds: one sample treated with anti-Sox6, a second treated with normal rabbit IgG, and the third sample without Ab. The last two were used as negative controls.
T20345	9483-9667	Sentence	denotes	One-tenth of the sample was set aside for input control, and the remaining sample was precleared with protein A-Sepharose (Amersham Biosciences, Piscataway, New Jersey, United States).
T20344	9427-9482	Sentence	denotes	DNA-protein complexes were sonicated to 200 and 600 bp.
T20343	9057-9426	Sentence	denotes	Cells from MEL cells (4 × 107) or fetal liver cells from three 15.5-dpc WT mice were treated with 1% formaldehyde for 10 min at 37 °C, rinsed in ice-cold 1× Hanks' balanced salt solution with 0.1% EDTA containing protease inhibitors, collected by centrifugation at 4 °C, resuspended in a SDS lysis buffer containing protease inhibitors, and incubated on ice for 10 min.
T20342	9017-9056	Sentence	denotes	As described by Nouzova [53], in brief:
T20341	9005-9016	Sentence	denotes	ChIP assay.
T19661	8952-9003	Sentence	denotes	5′AATGCAGtgccAAGGGTCAGAACATTGTCTGCGAAG3′ (MHB1650).
T19660	8875-8951	Sentence	denotes	5′AATGCAGAACAAAGGGTCAGAtgagTGTCTGCGAAG3′ (MHB1648); for mutant probe 3 (M3):
T19659	8805-8874	Sentence	denotes	5′AACAAAGGGTCAGAACATTGTCTGCGAAG3′ (MHB1644); for mutant probe 2 (M2):
T19658	8728-8804	Sentence	denotes	5′AATGCAGAACAAAGGGTCAGAACATTGTCTGCGAAG3′ (MHB1556); for mutant probe 1 (M1):
T19657	8704-8727	Sentence	denotes	For the 36-bp WT probe:
T19656	8613-8703	Sentence	denotes	The DNA sequences of the oligonucleotides are as follows (only forward oligos are listed):
T19655	8511-8612	Sentence	denotes	Antibodies used for supershift analyses included c-Myc, HA, and Sox6 antibodies (described as above).
T19654	8378-8510	Sentence	denotes	Electrophoresis was performed at a constant 19 mAmp for 4–8 h at room temperature, and the gels were dried prior to autoradiography.
T19653	8213-8377	Sentence	denotes	Following addition of the radiolabeled probe, the samples were incubated for 30 min or 60 min at room temperature and loaded on a 4% or 6% (w/v) polyacrylamide gel.
T19652	8012-8212	Sentence	denotes	For competition or supershift assays, the indicated unlabelled oligonucleotide competitor (200-fold molar excess) or antibody (3 μl) was added 30 min to 60 min prior to addition of radiolabeled probe.
T19651	7871-8011	Sentence	denotes	100 mM NaCl, 10% glycerol, 200 ng/μl BSA, 50 ng/μl poly (dI-dC) or poly (dG-dC), 10 mM HEPES (pH 7), 0.1 mM EDTA, 0.25 mM DTT, 0.6 mM MgCl2.
T19650	7717-7870	Sentence	denotes	EMSA was performed with 5 μg of nuclear proteins from MEL cells or 3 μl of in vitro-translated Sox6 along with the reticulocyte lysate in binding buffer:
T19649	7592-7716	Sentence	denotes	Single-stranded complementary oligonucleotides were annealed and end-labeled with [γ-32P] ATP with T4 polynucleotide kinase.
T19648	7586-7591	Sentence	denotes	EMSA.
T19123	7051-7135	Sentence	denotes	A vector without the Sox6 coding sequence was also translated as a negative control.
T19122	6895-7050	Sentence	denotes	The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega).
T19121	6793-6894	Sentence	denotes	The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15].
T19120	6672-6792	Sentence	denotes	Nuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States).
T19119	6614-6671	Sentence	denotes	Nuclear protein extract and in vitro translation of Sox6.
T19404	7385-7584	Sentence	denotes	All Sox6 antibodies generated similar results. c-Myc antibody was purchased from Invitrogen. Normal rabbit IgG antibody was obtained from Upstate Biotechnology (Lake Placid, New York, United States).
T19403	7149-7384	Sentence	denotes	Sox6 antibodies used in this study were either kindly provided by Dr. Enzo Lalli (Université Louis Pasteur, France [7]) or commercially obtained (Catalog No. sc-17332 X, Santa Cruz Biotechnology, Santa Cruz, California, United States).
T19402	7137-7148	Sentence	denotes	Antibodies.
T18709	6473-6612	Sentence	denotes	In assays of dosage effect, we used 200 ng, 500 ng, and 1000 ng). pRL-CMV 15ng (Promega) was used as a control for transfection efficiency.
T18708	6332-6472	Sentence	denotes	Cells were transfected with ɛy promoter reporter constructs (500 ng) along with either empty vector or Sox6 overexpression vector (1000 ng).
T18707	6198-6331	Sentence	denotes	GM979 cells (4 × 105) in log phase of growth were transfected with plasmids by FuGENE6 (Roche, Indianapolis, Indiana, United States).
T18706	6105-6197	Sentence	denotes	MEL cells were cultured in DMEM supplemented as above (without heat inactivating the serum).
T18705	5799-6104	Sentence	denotes	GM979 cells (Coriell Cell Repositories, Camden, New Jersey, United States) were cultured in Ham's F12 with 2 mM L-glutamine supplemented with heat-inactivated 10% fetal calf serum (Ivitrogen, Carlsbad, California, United States), penicillin (100 units/ml), streptomycin 100 μg/ml), and L-glutamine (2 mM).
T18704	5768-5798	Sentence	denotes	Cell culture and transfection.
T18455	5623-5766	Sentence	denotes	Blots were washed with 0.2× SSC, 1% SDS at 60 °C prior to exposure to X-ray film (Kodak, Rochester, New York, United States) at −80 °C for 6 d.
T18454	5538-5622	Sentence	denotes	The hybridization was performed in phosphate buffered 7% SDS hybridization solution.
T18453	5234-5537	Sentence	denotes	A mouse embryonic tissue Northern blot filter (Seegene, Rockville, Maryland, United States) was hybridized with a Sox6 probe generated by RT-PCR (nucleotides 1353–1927) and labeled with [α-32P]dCTP, by random primer labeling (RediprimeII; Amersham Biosciences, Buckinghamshire, England, United Kingdom).
T18452	5219-5233	Sentence	denotes	Northern blot.
T17745	3701-3895	Sentence	denotes	Embryos were fixed overnight by immersion in 4% paraformaldehyde, embedded in paraffin, sectioned at 5 μm, and adhered to charge modified slides (VWR, West Chester, Pennsylvania, United States).
T17744	3558-3700	Sentence	denotes	Antisense probes were designed to murine ɛy globin nucleotides 509–584; βmaj globin nucleotides 458–549; and mouse Sox6 nucleotides 1353–1927.
T17743	3535-3557	Sentence	denotes	In situ hybridization.
T17156	3352-3533	Sentence	denotes	Relative quantitative values were calculated in the ABI Prism 7000 SDS Software (Applied Biosystems) and normalized to GAPDH in Microsoft Excel (Redmond, Washington, United States).
T17155	3307-3351	Sentence	denotes	Triplicates were done for each PCR reaction.
T17154	3222-3306	Sentence	denotes	Standard curve analyses were performed to test the efficiency of the amplifications.
T17153	3168-3221	Sentence	denotes	GAPDH mRNA levels were used as control for input RNA.
T17152	3092-3167	Sentence	denotes	All PCR was performed in a 25-μl reaction with 12.5 μl SYBR green supermix.
T17151	2856-3091	Sentence	denotes	Using the SYBR green supermix kit with ROX (Bio-Rad, Hercules, California, United States), PCR amplification was run on an ABI7000 (Applied Biosystems, Foster City, California, United States) at the University of Arizona core facility.
T17150	2791-2855	Sentence	denotes	5′ATGGCCTGAATCACTTGGAC3′; and MHB1675, 5′ACGATCATATTGCCCAGGAG3′.
T17149	2761-2790	Sentence	denotes	For βmaj/min globin: MHB1674:
T17148	2671-2760	Sentence	denotes	For βH1 globin: MHB1672, 5′TGGACAACCTCAAGGAGACC3′; and MHB1673, 5′ACCTCTGGGGTGAATTCCTT3′.
T17147	2582-2670	Sentence	denotes	For ζ globin: MHB1668, 5′CTACCCCCAGACGAAGACCTA3′; and MHB1669, 5′CTTAACCGCATCCCCTACGG3′.
T17146	2491-2581	Sentence	denotes	For ɛy globin: MHB1666, 5′TGGCCTGTGGAGTAAGGTCAA3′; and MHB1667, 5′GAAGCAGAGGACAAGTTCCCA3′.
T17145	2415-2490	Sentence	denotes	All primers were searched against the NCBI database to confirm specificity.
T17144	2328-2414	Sentence	denotes	Primers for cDNA PCR amplification of globin genes were obtained from Primerbank [51].
T17143	2285-2327	Sentence	denotes	RNA was first reverse transcribed to cDNA.
T17142	2256-2284	Sentence	denotes	Quantitation of globin mRNA.
T16008	1936-2254	Sentence	denotes	Forward primers used to generate these mutagenized ɛy promoter reporter constructs include: MHB1661, 5′CCGCTCGAGAATGCAGTGCCAAGGGTCAGAACATTGTCTGCGAAG3′ (−63 to −19); MHB1662, 5′CCGCTCGAGAATGCAGAACAAAGGGTCAGATGAGTGTCTGCGAAGAA3′ (−63 to −16); and MHB1663, 5′CCGCTCGAGAATGCATGCCAAGGGTCAGATGAGTGTCTGCGAAGAA 3′ (−63 to −18).
T16007	1851-1935	Sentence	denotes	Mutagenesis of Sox/Sox6 consensus binding sites of the ɛy promoter were done by PCR.
T16006	1703-1850	Sentence	denotes	A truncated version of the Sox6 overexpression construct (Sox6-ΔHMG-pcDNA3.1) that lacks the HMG domain was generated, as described by others [32].
T16005	1654-1702	Sentence	denotes	Sox6-pcDNA3.1 [15] was used to overexpress Sox6.
T16004	1518-1653	Sentence	denotes	All forward primers were used in combination with the reverse primer HindIII site: MHB1477, 5′CGGAAGCTTGGGAGGTTGCTGGTGA3′ (+45 to +20).
T16003	1124-1517	Sentence	denotes	Forward primers with an XhoI site include: MHB1457, 5′CCGCTCGAGTGCTAGGCAAACACTCA3′ (−2077 to −2052); MHB1503, 5′CCGCTCGAGTCTCTACACTGTCACTCCCTG3′ (−634 to −605); MHB1505, 5′CCGCTCGAGGGAGCCAAAAAAAGAATGC3′ (−197 to −169); MHB1506, 5′CCGCTCGAGCTGACCAATGGCTTCAAAG3′ (−85 to −58); MHB1532, 5′CCGCTCGAGAATGCAGAACAAAGGGTCAGA3′ (−63 to −34); and MHB1507, 5′CCGCTCGAGGTCTGCGAAGAATAAAAGGC 3′ (−37 to −9).
T16002	1047-1123	Sentence	denotes	A series of deletion constructs of the ɛy promoter were generated similarly.
T16001	814-1046	Sentence	denotes	A 2.5-kb SstI/XhoI fragment of μLCRβ 9.3 (micro LCR; [31]) was then inserted upstream of the ɛy promoter in the above pGL3 Basic plasmid, resulting in a reporter construct in which luciferase expression is driven by the ɛy promoter.
T16000	616-813	Sentence	denotes	The PCR primers contained XhoI and HindIII sites that were used to clone the ɛy promoter fragment upstream of the firefly luciferase gene in pGL3 Basic (Promega, Madison, Wisconsin, United States).
T15999	499-615	Sentence	denotes	The transcriptional start site is based on the longest cDNA in the Fantom (Functional Annotation of Mouse) database.
T15998	433-498	Sentence	denotes	Nucleotide numbering is relative to the transcription start site.
T15997	159-432	Sentence	denotes	A 2.2-kb fragment upstream of the ɛy globin initiation codon (ATG) was used, because it has been shown that all sequences required for ɛ gene silencing are located within a 3.7-kb EcoRI fragment containing about 2 kb of sequence upstream of the ɛ globin gene cap site [50].
T15996	45-158	Sentence	denotes	The ɛy promoter deletion reporter plasmid (E-luc) was generated by PCR amplification of the ɛy proximal promoter.
T15995	23-44	Sentence	denotes	Plasmid construction.
T18184	5187-5217	Sentence	denotes	Original images are available.
T18183	5150-5186	Sentence	denotes	The camera was a Nikon Coolpix 4300.
T18182	5029-5149	Sentence	denotes	Objectives used were E Plan 40/0.65 160/0.17 Nikon (40× objective), E Plan 100/1.25 oil 160/0.17 Nikon (100× objective).
T18181	4974-5028	Sentence	denotes	Images were obtained with Nikon Labophot-2 microscope.
T18180	4898-4973	Sentence	denotes	Liver samples (at 14.5 dpc and 18.5 dpc) were prepared in a similar manner.
T18179	4706-4897	Sentence	denotes	For whole mount analysis, 14.5-dpc WT and mutant embryos, and postnatal day–10.5 mice were fixed in 10% formalin, paraffin-embedded, sectioned at 5 μm, and stained with hematoxylin and eosin.
T18178	4658-4705	Sentence	denotes	The slides were Wright-stained and read by DAF.
T18177	4550-4657	Sentence	denotes	18.5-dpc embryos were exsanguinated and peripheral blood smears were prepared from both mutant and WT mice.
T18176	4539-4549	Sentence	denotes	Histology.
T17750	4507-4537	Sentence	denotes	Original images are available.
T17749	4300-4506	Sentence	denotes	Images were processed, pseudocolored, and combined using Photoshop (Adobe, San Jose, California, United States) software with Fovea Pro (Reindeer Graphics, Asheville, North Carolina, United States) plugins.
T17748	4244-4299	Sentence	denotes	Objectives used were 1× (NA = 0.04) and 10× (NA = 0.5).
T17747	4018-4243	Sentence	denotes	Darkfield and brightfield images were obtained with a Nikon Optiphot microscope (Nikon, Melville, New York, United States) and SPOT RT-Slider digital camera (Diagnostic Instruments, Sterling Heights, Michigan, United States).
T17746	3896-4017	Sentence	denotes	Slides were processed for in situ hybridization as described [52] using in vitro transcribed RNA probes labeled with 33P.
T250	0-21	Sentence	denotes	Materials and Methods
T251	23-44	Sentence	denotes	Plasmid construction.
T252	45-158	Sentence	denotes	The ɛy promoter deletion reporter plasmid (E-luc) was generated by PCR amplification of the ɛy proximal promoter.
T253	159-432	Sentence	denotes	A 2.2-kb fragment upstream of the ɛy globin initiation codon (ATG) was used, because it has been shown that all sequences required for ɛ gene silencing are located within a 3.7-kb EcoRI fragment containing about 2 kb of sequence upstream of the ɛ globin gene cap site [50].
T254	433-498	Sentence	denotes	Nucleotide numbering is relative to the transcription start site.
T255	499-615	Sentence	denotes	The transcriptional start site is based on the longest cDNA in the Fantom (Functional Annotation of Mouse) database.
T256	616-813	Sentence	denotes	The PCR primers contained XhoI and HindIII sites that were used to clone the ɛy promoter fragment upstream of the firefly luciferase gene in pGL3 Basic (Promega, Madison, Wisconsin, United States).
T257	814-1046	Sentence	denotes	A 2.5-kb SstI/XhoI fragment of μLCRβ 9.3 (micro LCR; [31]) was then inserted upstream of the ɛy promoter in the above pGL3 Basic plasmid, resulting in a reporter construct in which luciferase expression is driven by the ɛy promoter.
T258	1047-1123	Sentence	denotes	A series of deletion constructs of the ɛy promoter were generated similarly.
T259	1124-1517	Sentence	denotes	Forward primers with an XhoI site include: MHB1457, 5′CCGCTCGAGTGCTAGGCAAACACTCA3′ (−2077 to −2052); MHB1503, 5′CCGCTCGAGTCTCTACACTGTCACTCCCTG3′ (−634 to −605); MHB1505, 5′CCGCTCGAGGGAGCCAAAAAAAGAATGC3′ (−197 to −169); MHB1506, 5′CCGCTCGAGCTGACCAATGGCTTCAAAG3′ (−85 to −58); MHB1532, 5′CCGCTCGAGAATGCAGAACAAAGGGTCAGA3′ (−63 to −34); and MHB1507, 5′CCGCTCGAGGTCTGCGAAGAATAAAAGGC 3′ (−37 to −9).
T260	1518-1653	Sentence	denotes	All forward primers were used in combination with the reverse primer HindIII site: MHB1477, 5′CGGAAGCTTGGGAGGTTGCTGGTGA3′ (+45 to +20).
T261	1654-1702	Sentence	denotes	Sox6-pcDNA3.1 [15] was used to overexpress Sox6.
T262	1703-1850	Sentence	denotes	A truncated version of the Sox6 overexpression construct (Sox6-ΔHMG-pcDNA3.1) that lacks the HMG domain was generated, as described by others [32].
T263	1851-1935	Sentence	denotes	Mutagenesis of Sox/Sox6 consensus binding sites of the ɛy promoter were done by PCR.
T264	1936-2254	Sentence	denotes	Forward primers used to generate these mutagenized ɛy promoter reporter constructs include: MHB1661, 5′CCGCTCGAGAATGCAGTGCCAAGGGTCAGAACATTGTCTGCGAAG3′ (−63 to −19); MHB1662, 5′CCGCTCGAGAATGCAGAACAAAGGGTCAGATGAGTGTCTGCGAAGAA3′ (−63 to −16); and MHB1663, 5′CCGCTCGAGAATGCATGCCAAGGGTCAGATGAGTGTCTGCGAAGAA 3′ (−63 to −18).
T265	2256-2284	Sentence	denotes	Quantitation of globin mRNA.
T266	2285-2327	Sentence	denotes	RNA was first reverse transcribed to cDNA.
T267	2328-2414	Sentence	denotes	Primers for cDNA PCR amplification of globin genes were obtained from Primerbank [51].
T268	2415-2490	Sentence	denotes	All primers were searched against the NCBI database to confirm specificity.
T269	2491-2581	Sentence	denotes	For ɛy globin: MHB1666, 5′TGGCCTGTGGAGTAAGGTCAA3′; and MHB1667, 5′GAAGCAGAGGACAAGTTCCCA3′.
T270	2582-2670	Sentence	denotes	For ζ globin: MHB1668, 5′CTACCCCCAGACGAAGACCTA3′; and MHB1669, 5′CTTAACCGCATCCCCTACGG3′.
T271	2671-2760	Sentence	denotes	For βH1 globin: MHB1672, 5′TGGACAACCTCAAGGAGACC3′; and MHB1673, 5′ACCTCTGGGGTGAATTCCTT3′.
T272	2761-2790	Sentence	denotes	For βmaj/min globin: MHB1674:
T273	2791-2855	Sentence	denotes	5′ATGGCCTGAATCACTTGGAC3′; and MHB1675, 5′ACGATCATATTGCCCAGGAG3′.
T274	2856-3091	Sentence	denotes	Using the SYBR green supermix kit with ROX (Bio-Rad, Hercules, California, United States), PCR amplification was run on an ABI7000 (Applied Biosystems, Foster City, California, United States) at the University of Arizona core facility.
T275	3092-3167	Sentence	denotes	All PCR was performed in a 25-μl reaction with 12.5 μl SYBR green supermix.
T276	3168-3221	Sentence	denotes	GAPDH mRNA levels were used as control for input RNA.
T277	3222-3306	Sentence	denotes	Standard curve analyses were performed to test the efficiency of the amplifications.
T278	3307-3351	Sentence	denotes	Triplicates were done for each PCR reaction.
T279	3352-3533	Sentence	denotes	Relative quantitative values were calculated in the ABI Prism 7000 SDS Software (Applied Biosystems) and normalized to GAPDH in Microsoft Excel (Redmond, Washington, United States).
T280	3535-3557	Sentence	denotes	In situ hybridization.
T281	3558-3700	Sentence	denotes	Antisense probes were designed to murine ɛy globin nucleotides 509–584; βmaj globin nucleotides 458–549; and mouse Sox6 nucleotides 1353–1927.
T282	3701-3895	Sentence	denotes	Embryos were fixed overnight by immersion in 4% paraformaldehyde, embedded in paraffin, sectioned at 5 μm, and adhered to charge modified slides (VWR, West Chester, Pennsylvania, United States).
T283	3896-4017	Sentence	denotes	Slides were processed for in situ hybridization as described [52] using in vitro transcribed RNA probes labeled with 33P.
T284	4018-4243	Sentence	denotes	Darkfield and brightfield images were obtained with a Nikon Optiphot microscope (Nikon, Melville, New York, United States) and SPOT RT-Slider digital camera (Diagnostic Instruments, Sterling Heights, Michigan, United States).
T285	4244-4299	Sentence	denotes	Objectives used were 1× (NA = 0.04) and 10× (NA = 0.5).
T286	4300-4506	Sentence	denotes	Images were processed, pseudocolored, and combined using Photoshop (Adobe, San Jose, California, United States) software with Fovea Pro (Reindeer Graphics, Asheville, North Carolina, United States) plugins.
T287	4507-4537	Sentence	denotes	Original images are available.
T288	4539-4549	Sentence	denotes	Histology.
T289	4550-4657	Sentence	denotes	18.5-dpc embryos were exsanguinated and peripheral blood smears were prepared from both mutant and WT mice.
T290	4658-4705	Sentence	denotes	The slides were Wright-stained and read by DAF.
T291	4706-4897	Sentence	denotes	For whole mount analysis, 14.5-dpc WT and mutant embryos, and postnatal day–10.5 mice were fixed in 10% formalin, paraffin-embedded, sectioned at 5 μm, and stained with hematoxylin and eosin.
T292	4898-4973	Sentence	denotes	Liver samples (at 14.5 dpc and 18.5 dpc) were prepared in a similar manner.
T293	4974-5028	Sentence	denotes	Images were obtained with Nikon Labophot-2 microscope.
T294	5029-5149	Sentence	denotes	Objectives used were E Plan 40/0.65 160/0.17 Nikon (40× objective), E Plan 100/1.25 oil 160/0.17 Nikon (100× objective).
T295	5150-5186	Sentence	denotes	The camera was a Nikon Coolpix 4300.
T296	5187-5217	Sentence	denotes	Original images are available.
T297	5219-5233	Sentence	denotes	Northern blot.
T298	5234-5537	Sentence	denotes	A mouse embryonic tissue Northern blot filter (Seegene, Rockville, Maryland, United States) was hybridized with a Sox6 probe generated by RT-PCR (nucleotides 1353–1927) and labeled with [α-32P]dCTP, by random primer labeling (RediprimeII; Amersham Biosciences, Buckinghamshire, England, United Kingdom).
T299	5538-5622	Sentence	denotes	The hybridization was performed in phosphate buffered 7% SDS hybridization solution.
T300	5623-5766	Sentence	denotes	Blots were washed with 0.2× SSC, 1% SDS at 60 °C prior to exposure to X-ray film (Kodak, Rochester, New York, United States) at −80 °C for 6 d.
T301	5768-5798	Sentence	denotes	Cell culture and transfection.
T302	5799-6104	Sentence	denotes	GM979 cells (Coriell Cell Repositories, Camden, New Jersey, United States) were cultured in Ham's F12 with 2 mM L-glutamine supplemented with heat-inactivated 10% fetal calf serum (Ivitrogen, Carlsbad, California, United States), penicillin (100 units/ml), streptomycin 100 μg/ml), and L-glutamine (2 mM).
T303	6105-6197	Sentence	denotes	MEL cells were cultured in DMEM supplemented as above (without heat inactivating the serum).
T304	6198-6331	Sentence	denotes	GM979 cells (4 × 105) in log phase of growth were transfected with plasmids by FuGENE6 (Roche, Indianapolis, Indiana, United States).
T305	6332-6472	Sentence	denotes	Cells were transfected with ɛy promoter reporter constructs (500 ng) along with either empty vector or Sox6 overexpression vector (1000 ng).
T306	6473-6612	Sentence	denotes	In assays of dosage effect, we used 200 ng, 500 ng, and 1000 ng). pRL-CMV 15ng (Promega) was used as a control for transfection efficiency.
T307	6614-6671	Sentence	denotes	Nuclear protein extract and in vitro translation of Sox6.
T308	6672-6792	Sentence	denotes	Nuclear extracts were prepared from MEL cells (2 × 107) using a kit (Active Motif, Carlsbad, California, United States).
T309	6793-6894	Sentence	denotes	The Sox6 in vitro translation expression vector, tagged with c-Myc and HA, was described before [15].
T310	6895-7050	Sentence	denotes	The translation was performed in a reticulocyte lysate based in vitro translational system (TNT® Quick Coupled Transcription/Translation Systems, Promega).
T311	7051-7135	Sentence	denotes	A vector without the Sox6 coding sequence was also translated as a negative control.
T312	7137-7148	Sentence	denotes	Antibodies.
T313	7149-7384	Sentence	denotes	Sox6 antibodies used in this study were either kindly provided by Dr. Enzo Lalli (Université Louis Pasteur, France [7]) or commercially obtained (Catalog No. sc-17332 X, Santa Cruz Biotechnology, Santa Cruz, California, United States).
T314	7385-7477	Sentence	denotes	All Sox6 antibodies generated similar results. c-Myc antibody was purchased from Invitrogen.
T315	7478-7584	Sentence	denotes	Normal rabbit IgG antibody was obtained from Upstate Biotechnology (Lake Placid, New York, United States).
T316	7586-7591	Sentence	denotes	EMSA.
T317	7592-7716	Sentence	denotes	Single-stranded complementary oligonucleotides were annealed and end-labeled with [γ-32P] ATP with T4 polynucleotide kinase.
T318	7717-7870	Sentence	denotes	EMSA was performed with 5 μg of nuclear proteins from MEL cells or 3 μl of in vitro-translated Sox6 along with the reticulocyte lysate in binding buffer:
T319	7871-8011	Sentence	denotes	100 mM NaCl, 10% glycerol, 200 ng/μl BSA, 50 ng/μl poly (dI-dC) or poly (dG-dC), 10 mM HEPES (pH 7), 0.1 mM EDTA, 0.25 mM DTT, 0.6 mM MgCl2.
T320	8012-8212	Sentence	denotes	For competition or supershift assays, the indicated unlabelled oligonucleotide competitor (200-fold molar excess) or antibody (3 μl) was added 30 min to 60 min prior to addition of radiolabeled probe.
T321	8213-8377	Sentence	denotes	Following addition of the radiolabeled probe, the samples were incubated for 30 min or 60 min at room temperature and loaded on a 4% or 6% (w/v) polyacrylamide gel.
T322	8378-8510	Sentence	denotes	Electrophoresis was performed at a constant 19 mAmp for 4–8 h at room temperature, and the gels were dried prior to autoradiography.
T323	8511-8612	Sentence	denotes	Antibodies used for supershift analyses included c-Myc, HA, and Sox6 antibodies (described as above).
T324	8613-8703	Sentence	denotes	The DNA sequences of the oligonucleotides are as follows (only forward oligos are listed):
T325	8704-8727	Sentence	denotes	For the 36-bp WT probe:
T326	8728-8804	Sentence	denotes	5′AATGCAGAACAAAGGGTCAGAACATTGTCTGCGAAG3′ (MHB1556); for mutant probe 1 (M1):
T327	8805-8874	Sentence	denotes	5′AACAAAGGGTCAGAACATTGTCTGCGAAG3′ (MHB1644); for mutant probe 2 (M2):
T328	8875-8951	Sentence	denotes	5′AATGCAGAACAAAGGGTCAGAtgagTGTCTGCGAAG3′ (MHB1648); for mutant probe 3 (M3):
T329	8952-9003	Sentence	denotes	5′AATGCAGtgccAAGGGTCAGAACATTGTCTGCGAAG3′ (MHB1650).
T330	9005-9016	Sentence	denotes	ChIP assay.
T331	9017-9056	Sentence	denotes	As described by Nouzova [53], in brief:
T332	9057-9426	Sentence	denotes	Cells from MEL cells (4 × 107) or fetal liver cells from three 15.5-dpc WT mice were treated with 1% formaldehyde for 10 min at 37 °C, rinsed in ice-cold 1× Hanks' balanced salt solution with 0.1% EDTA containing protease inhibitors, collected by centrifugation at 4 °C, resuspended in a SDS lysis buffer containing protease inhibitors, and incubated on ice for 10 min.
T333	9427-9482	Sentence	denotes	DNA-protein complexes were sonicated to 200 and 600 bp.
T334	9483-9667	Sentence	denotes	One-tenth of the sample was set aside for input control, and the remaining sample was precleared with protein A-Sepharose (Amersham Biosciences, Piscataway, New Jersey, United States).
T335	9668-9835	Sentence	denotes	Following preclearing, the samples were split into thirds: one sample treated with anti-Sox6, a second treated with normal rabbit IgG, and the third sample without Ab.
T336	9836-9880	Sentence	denotes	The last two were used as negative controls.
T337	9881-10004	Sentence	denotes	The chromatin-antibody complexes were eluted, and the DNA protein cross-links were reversed with 5 M NaCl at 65 °C for 4 h.
T338	10005-10084	Sentence	denotes	Input DNA or immunoprecipitated DNA was used as a template in the PCR reaction.
T339	10085-10310	Sentence	denotes	PCR amplification of the ɛy promoter was performed and yielded a 172-bp amplicon, corresponding to nucleotides −31 to +140 of the ɛy promoter (primers MHB1688, 5′CGAAGAATAAAAGGCCACCA3′; and MHB1689, 5′GCTTCACCACCAACCTCTTC3′).
T340	10311-10360	Sentence	denotes	PCR was performed under the following conditions:
T341	10361-10504	Sentence	denotes	95 °C for 15 min followed by 30 cycles at 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 45 s, ending with a final extension at 72 °C for 5 min.

simple1

Id	Subject	Object	Predicate	Lexical cue
T20381	10215-10217	Protein	denotes	ɛy
T20380	10110-10112	Protein	denotes	ɛy
T20379	9756-9760	Protein	denotes	Sox6
T20378	9585-9594	Protein	denotes	protein A
T19693	8575-8579	Protein	denotes	Sox6
T19692	8567-8569	Protein	denotes	HA
T19691	8560-8565	Protein	denotes	c-Myc
T19690	7908-7911	Protein	denotes	BSA
T19689	7812-7816	Protein	denotes	Sox6
T19688	7691-7715	Protein	denotes	T4 polynucleotide kinase
T19149	7072-7076	Protein	denotes	Sox6
T19148	6864-6866	Protein	denotes	HA
T19147	6854-6859	Protein	denotes	c-Myc
T19146	6797-6801	Protein	denotes	Sox6
T19145	6666-6670	Protein	denotes	Sox6
T19426	7432-7437	Protein	denotes	c-Myc
T19425	7389-7393	Protein	denotes	Sox6
T19424	7149-7153	Protein	denotes	Sox6
T18722	6435-6439	Protein	denotes	Sox6
T18721	6360-6362	Protein	denotes	ɛy
T18460	5348-5352	Protein	denotes	Sox6
T17765	3673-3677	Protein	denotes	Sox6
T17764	3630-3641	Protein	denotes	βmaj globin
T17763	3599-3608	Protein	denotes	ɛy globin
T17190	3471-3476	Protein	denotes	GAPDH
T17189	3168-3173	Protein	denotes	GAPDH
T17188	2770-2780	Protein	denotes	min globin
T17187	2765-2769	Protein	denotes	βmaj
T17186	2675-2685	Protein	denotes	βH1 globin
T17185	2586-2594	Protein	denotes	ζ globin
T17184	2495-2504	Protein	denotes	ɛy globin
T16072	1906-1908	Protein	denotes	ɛy
T16071	1761-1765	Protein	denotes	Sox6
T16070	1730-1734	Protein	denotes	Sox6
T16069	1697-1701	Protein	denotes	Sox6
T16068	1654-1658	Protein	denotes	Sox6
T16067	1086-1088	Protein	denotes	ɛy
T16066	1034-1036	Protein	denotes	ɛy
T16065	995-1005	Protein	denotes	luciferase
T16064	907-909	Protein	denotes	ɛy
T16063	738-748	Protein	denotes	luciferase
T16062	693-695	Protein	denotes	ɛy
T16061	404-412	Protein	denotes	ɛ globin
T16060	294-295	Protein	denotes	ɛ
T16059	193-202	Protein	denotes	ɛy globin
T16058	137-139	Protein	denotes	ɛy
T16057	49-51	Protein	denotes	ɛy

BioNLP16_DUT

Id	Subject	Object	Predicate	Lexical cue
T21019	9585-9594	Protein	denotes	protein A
T21022	10215-10217	Protein	denotes	ɛy
T21021	10110-10112	Protein	denotes	ɛy
T21020	9756-9760	Protein	denotes	Sox6
T20316	8575-8579	Protein	denotes	Sox6
T20315	8567-8569	Protein	denotes	HA
T20314	8560-8565	Protein	denotes	c-Myc
T20313	7908-7911	Protein	denotes	BSA
T20312	7812-7816	Protein	denotes	Sox6
T20311	7691-7715	Protein	denotes	T4 polynucleotide kinase
T19620	7432-7437	Protein	denotes	c-Myc
T19619	7389-7393	Protein	denotes	Sox6
T19618	7149-7153	Protein	denotes	Sox6
T19096	6440-6454	Gene_expression	denotes	overexpression
T19095	6435-6439	Protein	denotes	Sox6
T19094	6360-6362	Protein	denotes	ɛy
T19384	6823-6833	Gene_expression	denotes	expression
T19383	7072-7076	Protein	denotes	Sox6
T19382	6864-6866	Protein	denotes	HA
T19381	6854-6859	Protein	denotes	c-Myc
T19380	6797-6801	Protein	denotes	Sox6
T19379	6666-6670	Protein	denotes	Sox6
T18692	5348-5352	Protein	denotes	Sox6
T18169	3673-3677	Protein	denotes	Sox6
T18168	3630-3641	Protein	denotes	βmaj globin
T18167	3599-3608	Protein	denotes	ɛy globin
T17722	3471-3476	Protein	denotes	GAPDH
T17721	3168-3173	Protein	denotes	GAPDH
T17720	2770-2780	Protein	denotes	min globin
T17719	2765-2769	Protein	denotes	βmaj
T17718	2675-2685	Protein	denotes	βH1 globin
T17717	2586-2594	Protein	denotes	ζ globin
T17716	2495-2504	Protein	denotes	ɛy globin
T17096	1685-1696	Gene_expression	denotes	overexpress
T17095	1685-1696	Positive_regulation	denotes	overexpress
T17094	1020-1026	Positive_regulation	denotes	driven
T17093	1006-1016	Gene_expression	denotes	expression
T17092	301-310	Negative_regulation	denotes	silencing
T17091	1906-1908	Protein	denotes	ɛy
T17090	1761-1765	Protein	denotes	Sox6
T17089	1730-1734	Protein	denotes	Sox6
T17088	1697-1701	Protein	denotes	Sox6
T17087	1654-1658	Protein	denotes	Sox6
T17086	1086-1088	Protein	denotes	ɛy
T17085	995-1005	Protein	denotes	luciferase
T17084	907-909	Protein	denotes	ɛy
T17083	1034-1036	Protein	denotes	ɛy
T17082	738-748	Protein	denotes	luciferase
T17081	693-695	Protein	denotes	ɛy
T17080	404-412	Protein	denotes	ɛ globin
T17079	294-295	Protein	denotes	ɛ
T17078	193-202	Protein	denotes	ɛy globin
T17077	137-139	Protein	denotes	ɛy
T17076	49-51	Protein	denotes	ɛy
R11932	T17079	T17092	themeOf	ɛ,silencing
R11933	T17085	T17093	themeOf	luciferase,expression
R11934	T17088	T17096	themeOf	Sox6,overexpress
R11935	T17093	T17094	themeOf	expression,driven
R11936	T17096	T17095	themeOf	overexpress,overexpress
R13574	T19095	T19096	themeOf	Sox6,overexpression
R13771	T19380	T19384	themeOf	Sox6,expression

BioNLP16_Messiy

Id	Subject	Object	Predicate	Lexical cue
T20669	10215-10217	Protein	denotes	ɛy
T20668	10110-10112	Protein	denotes	ɛy
T20667	9756-9760	Protein	denotes	Sox6
T16456	693-695	Protein	denotes	ɛy
T16455	404-412	Protein	denotes	ɛ globin
T16454	294-295	Protein	denotes	ɛ
T16453	193-202	Protein	denotes	ɛy globin
T16452	137-139	Protein	denotes	ɛy
T16451	49-51	Protein	denotes	ɛy
T20666	9585-9594	Protein	denotes	protein A
T19965	8575-8579	Protein	denotes	Sox6
T19964	8567-8569	Protein	denotes	HA
T19963	8560-8565	Protein	denotes	c-Myc
T19962	7908-7911	Protein	denotes	BSA
T19961	7812-7816	Protein	denotes	Sox6
T19960	7691-7715	Protein	denotes	T4 polynucleotide kinase
T19508	7432-7437	Protein	denotes	c-Myc
T19507	7389-7393	Protein	denotes	Sox6
T19506	7149-7153	Protein	denotes	Sox6
T19249	6823-6833	Gene_expression	denotes	expression
T19248	7072-7076	Protein	denotes	Sox6
T19247	6864-6866	Protein	denotes	HA
T19246	6854-6859	Protein	denotes	c-Myc
T19245	6797-6801	Protein	denotes	Sox6
T19244	6666-6670	Protein	denotes	Sox6
T18891	6440-6454	Gene_expression	denotes	overexpression
T18890	6435-6439	Protein	denotes	Sox6
T18889	6360-6362	Protein	denotes	ɛy
T17948	3673-3677	Protein	denotes	Sox6
T17947	3630-3641	Protein	denotes	βmaj globin
T17946	3599-3608	Protein	denotes	ɛy globin
T18567	5348-5352	Protein	denotes	Sox6
T17403	3471-3476	Protein	denotes	GAPDH
T17402	3168-3173	Protein	denotes	GAPDH
T17401	2770-2780	Protein	denotes	min globin
T17400	2765-2769	Protein	denotes	βmaj
T17399	2675-2685	Protein	denotes	βH1 globin
T17398	2586-2594	Protein	denotes	ζ globin
T17397	2495-2504	Protein	denotes	ɛy globin
T16472	1735-1749	Positive_regulation	denotes	overexpression
T16471	1685-1696	Gene_expression	denotes	overexpress
T16470	1006-1016	Gene_expression	denotes	expression
T16469	281-289	Positive_regulation	denotes	required
T16468	301-310	Negative_regulation	denotes	silencing
T16467	61-69	Negative_regulation	denotes	deletion
T16466	1906-1908	Protein	denotes	ɛy
T16465	1761-1765	Protein	denotes	Sox6
T16464	1730-1734	Protein	denotes	Sox6
T16463	1697-1701	Protein	denotes	Sox6
T16462	1654-1658	Protein	denotes	Sox6
T16461	1086-1088	Protein	denotes	ɛy
T16460	995-1005	Protein	denotes	luciferase
T16459	907-909	Protein	denotes	ɛy
T16458	1034-1036	Protein	denotes	ɛy
T16457	738-748	Protein	denotes	luciferase
R11455	T16451	T16467	themeOf	ɛy,deletion
R11456	T16454	T16468	themeOf	ɛ,silencing
R11457	T16460	T16470	themeOf	luciferase,expression
R11458	T16463	T16471	themeOf	Sox6,overexpress
R11459	T16464	T16472	themeOf	Sox6,overexpression
R11460	T16468	T16469	themeOf	silencing,required
R13396	T18890	T18891	themeOf	Sox6,overexpression
R13668	T19245	T19249	themeOf	Sox6,expression

DLUT931

Id	Subject	Object	Predicate	Lexical cue
T20673	10215-10217	Protein	denotes	ɛy
T20672	10110-10112	Protein	denotes	ɛy
T20671	9756-9760	Protein	denotes	Sox6
T20670	9585-9594	Protein	denotes	protein A
T19978	7855-7862	Binding	denotes	binding
T19977	8575-8579	Protein	denotes	Sox6
T19976	8567-8569	Protein	denotes	HA
T19975	8560-8565	Protein	denotes	c-Myc
T19974	7908-7911	Protein	denotes	BSA
T19973	7812-7816	Protein	denotes	Sox6
T19972	7691-7715	Protein	denotes	T4 polynucleotide kinase
T19518	7432-7437	Protein	denotes	c-Myc
T19517	7389-7393	Protein	denotes	Sox6
T19516	7149-7153	Protein	denotes	Sox6
T19260	6823-6833	Gene_expression	denotes	expression
T19259	7072-7076	Protein	denotes	Sox6
T19258	6864-6866	Protein	denotes	HA
T19257	6854-6859	Protein	denotes	c-Myc
T19256	6797-6801	Protein	denotes	Sox6
T19255	6666-6670	Protein	denotes	Sox6
T18898	6440-6454	Gene_expression	denotes	overexpression
T18897	6435-6439	Protein	denotes	Sox6
T18896	6360-6362	Protein	denotes	ɛy
T18570	5348-5352	Protein	denotes	Sox6
T17957	3673-3677	Protein	denotes	Sox6
T17956	3630-3641	Protein	denotes	βmaj globin
T17955	3599-3608	Protein	denotes	ɛy globin
T17430	3471-3476	Protein	denotes	GAPDH
T17429	3168-3173	Protein	denotes	GAPDH
T17428	2770-2780	Protein	denotes	min globin
T17427	2765-2769	Protein	denotes	βmaj
T17426	2675-2685	Protein	denotes	βH1 globin
T17425	2586-2594	Protein	denotes	ζ globin
T17424	2495-2504	Protein	denotes	ɛy globin
T16542	1735-1749	Positive_regulation	denotes	overexpression
T16541	1685-1696	Gene_expression	denotes	overexpress
T16540	1006-1016	Gene_expression	denotes	expression
T16539	281-289	Positive_regulation	denotes	required
T16538	301-310	Negative_regulation	denotes	silencing
T16537	61-69	Negative_regulation	denotes	deletion
T16536	1906-1908	Protein	denotes	ɛy
T16535	1761-1765	Protein	denotes	Sox6
T16534	1730-1734	Protein	denotes	Sox6
T16533	1697-1701	Protein	denotes	Sox6
T16532	1654-1658	Protein	denotes	Sox6
T16531	1086-1088	Protein	denotes	ɛy
T16530	995-1005	Protein	denotes	luciferase
T16529	907-909	Protein	denotes	ɛy
T16528	1034-1036	Protein	denotes	ɛy
T16527	738-748	Protein	denotes	luciferase
T16526	693-695	Protein	denotes	ɛy
T16525	404-412	Protein	denotes	ɛ globin
T16524	294-295	Protein	denotes	ɛ
T16523	193-202	Protein	denotes	ɛy globin
T16522	137-139	Protein	denotes	ɛy
T16521	49-51	Protein	denotes	ɛy
R11469	T16521	T16537	themeOf	ɛy,deletion
R11470	T16524	T16538	themeOf	ɛ,silencing
R11471	T16530	T16540	themeOf	luciferase,expression
R11472	T16533	T16541	themeOf	Sox6,overexpress
R11473	T16534	T16542	themeOf	Sox6,overexpression
R11474	T16538	T16539	themeOf	silencing,required
R13397	T18897	T18898	themeOf	Sox6,overexpression
R13669	T19256	T19260	themeOf	Sox6,expression
R14198	T19973	T19978	themeOf	Sox6,binding

bionlp-st-ge-2016-test-ihmc

Id	Subject	Object	Predicate	Lexical cue
T21018	9931-9959	Localization	denotes	the DNA protein cross-links
T21017	9050-9062	Entity	denotes	brief: Cells
T21016	10018-10040	Entity	denotes	immunoprecipitated DNA
T21015	9114-9136	Protein	denotes	three 15.5-dpc WT mice
T21014	10000-10014	Entity	denotes	4 h. Input DNA
T21013	9751-9802	Protein	denotes	anti-Sox6, a second treated with normal rabbit IgG,
T21012	10181-10226	Entity	denotes	to nucleotides −31 to +140 of the ɛy promoter
T21011	9895-9903	Protein	denotes	antibody
T21010	9202-9210	Entity	denotes	ice-cold
T21009	9408-9414	Entity	denotes	on ice
T21008	10067-10083	Protein	denotes	the PCR reaction
T21007	9155-9181	Entity	denotes	1% formaldehyde for 10 min
T21006	9881-9913	Entity	denotes	The chromatin-antibody complexes
T21005	9978-9992	Entity	denotes	5 M NaCl at 65
T21004	9885-9894	Entity	denotes	chromatin
T21003	9935-9938	Entity	denotes	DNA
T21002	9373-9392	Protein	denotes	protease inhibitors
T21001	9254-9258	Entity	denotes	EDTA
T21000	10085-10088	Protein	denotes	PCR
T20999	9931-9958	Protein	denotes	the DNA protein cross-links
T20998	10311-10314	Protein	denotes	PCR
T20997	10215-10217	Protein	denotes	ɛy
T20996	10110-10112	Protein	denotes	ɛy
T20995	10103-10121	Entity	denotes	of the ɛy promoter
T20994	9595-9604	Entity	denotes	Sepharose
T20993	9343-9393	Protein	denotes	a SDS lysis buffer containing protease inhibitors,
T20992	9249-9289	Protein	denotes	0.1% EDTA containing protease inhibitors
T20991	9427-9448	Entity	denotes	DNA-protein complexes
T20990	10211-10226	Entity	denotes	the ɛy promoter
T20310	8575-8590	Entity	denotes	Sox6 antibodies
T20309	8567-8569	Protein	denotes	HA
T20308	8341-8376	Entity	denotes	a 4% or 6% (w/v) polyacrylamide gel
T20307	8129-8144	Protein	denotes	antibody (3 μl)
T20306	8511-8526	Entity	denotes	Antibodies used
T20305	7792-7816	Protein	denotes	in vitro-translated Sox6
T20304	7944-7949	Protein	denotes	dG-dC
T20303	8631-8654	Entity	denotes	of the oligonucleotides
T20302	7682-7715	Entity	denotes	ATP with T4 polynucleotide kinase
T20301	7691-7715	Protein	denotes	T4 polynucleotide kinase
T20300	7832-7844	Entity	denotes	reticulocyte
T20299	8341-8376	Entity	denotes	a 4% or 6% (w/v) polyacrylamide gel
T20298	8560-8565	Protein	denotes	c-Myc
T20297	7884-7896	Entity	denotes	10% glycerol
T20296	7592-7638	Entity	denotes	Single-stranded complementary oligonucleotides
T20295	8465-8473	Entity	denotes	the gels
T20294	7972-7997	Entity	denotes	0.1 mM EDTA, 0.25 mM DTT,
T20293	8064-8125	Entity	denotes	unlabelled oligonucleotide competitor (200-fold molar excess)
T20292	8800-8802	Entity	denotes	M1
T20291	8870-8872	Entity	denotes	M2
T20290	7908-7912	Protein	denotes	BSA,
T20289	7922-7934	Entity	denotes	poly (dI-dC)
T20288	8613-8669	Entity	denotes	The DNA sequences of the oligonucleotides are as follows
T20287	7938-7963	Entity	denotes	poly (dG-dC), 10 mM HEPES
T20286	7749-7780	Protein	denotes	nuclear proteins from MEL cells
T20285	8434-8459	Entity	denotes	4–8 h at room temperature
T20284	7985-7996	Entity	denotes	0.25 mM DTT
T20283	7938-7951	Entity	denotes	poly (dG-dC),
T20282	7871-7897	Entity	denotes	100 mM NaCl, 10% glycerol,
T20281	8411-8429	Entity	denotes	a constant 19 mAmp
T20280	7691-7693	Entity	denotes	T4
T20279	8575-8579	Protein	denotes	Sox6
T19617	7432-7476	Binding	denotes	c-Myc antibody was purchased from Invitrogen
T19616	7385-7404	Entity	denotes	All Sox6 antibodies
T19615	7492-7504	Entity	denotes	IgG antibody
T19614	7137-7147	Entity	denotes	Antibodies
T19613	7389-7393	Protein	denotes	Sox6
T19612	7432-7446	Entity	denotes	c-Myc antibody
T19611	7149-7153	Protein	denotes	Sox6
T19610	7149-7183	Entity	denotes	Sox6 antibodies used in this study
T19609	7432-7437	Protein	denotes	c-Myc
T19093	6435-6461	Gene_expression	denotes	Sox6 overexpression vector
T19092	6435-6461	Positive_regulation	denotes	Sox6 overexpression vector
T19091	6029-6039	Entity	denotes	penicillin
T19090	6056-6068	Entity	denotes	streptomycin
T19089	6435-6439	Protein	denotes	Sox6
T19088	5891-5945	Protein	denotes	Ham's F12 with 2 mM L-glutamine supplemented with heat
T19087	6198-6209	Entity	denotes	GM979 cells
T19086	5799-5810	Entity	denotes	GM979 cells
T19085	6085-6103	Entity	denotes	L-glutamine (2 mM)
T19084	6332-6337	Entity	denotes	Cells
T19083	6363-6371	Entity	denotes	promoter
T19082	6476-6499	Entity	denotes	assays of dosage effect
T19081	6109-6114	Entity	denotes	cells
T19080	5906-5922	Entity	denotes	2 mM L-glutamine
T19079	5820-5824	Entity	denotes	Cell
T19078	6539-6542	Protein	denotes	pRL
T19077	6355-6362	Protein	denotes	with ɛy
T19378	6642-6670	Translation	denotes	in vitro translation of Sox6
T19377	6930-6942	Entity	denotes	reticulocyte
T19376	6864-6866	Protein	denotes	HA
T19375	6672-6688	Entity	denotes	Nuclear extracts
T19374	6854-6859	Protein	denotes	c-Myc
T19373	6614-6637	Protein	denotes	Nuclear protein extract
T19372	6797-6801	Protein	denotes	Sox6
T19371	6663-6670	Protein	denotes	of Sox6
T19370	6614-6637	Entity	denotes	Nuclear protein extract
T19369	7072-7076	Protein	denotes	Sox6
T18691	5369-5402	Protein	denotes	by RT-PCR (nucleotides 1353–1927)
T18690	5595-5598	Protein	denotes	SDS
T18689	5573-5582	Entity	denotes	phosphate
T18688	5346-5402	Protein	denotes	a Sox6 probe generated by RT-PCR (nucleotides 1353–1927)
T18687	5427-5449	Entity	denotes	dCTP, by random primer
T18686	5656-5668	Protein	denotes	1% SDS at 60
T18445	4633-4656	Protein	denotes	both mutant and WT mice
T18444	4875-4886	Entity	denotes	hematoxylin
T18443	4929-4937	Entity	denotes	18.5 dpc
T18442	4768-4791	Protein	denotes	postnatal day–10.5 mice
T18441	4806-4838	Entity	denotes	10% formalin, paraffin-embedded,
T18440	4820-4837	Entity	denotes	paraffin-embedded
T18439	4916-4924	Entity	denotes	14.5 dpc
T17701	2272-2278	Protein	denotes	globin
T17700	2363-2378	Protein	denotes	of globin genes
T17699	2675-2685	Protein	denotes	βH1 globin
T17698	3211-3220	Protein	denotes	input RNA
T17697	3400-3413	Protein	denotes	the ABI Prism
T17696	3051-3090	Entity	denotes	the University of Arizona core facility
T17695	2495-2504	Protein	denotes	ɛy globin
T18166	3989-3992	Protein	denotes	RNA
T18165	3602-3653	Protein	denotes	globin nucleotides 509–584; βmaj globin nucleotides
T18164	3673-3677	Protein	denotes	Sox6
T18163	3896-3902	Entity	denotes	Slides
T18162	3602-3653	Entity	denotes	globin nucleotides 509–584; βmaj globin nucleotides
T18161	3779-3788	Entity	denotes	paraffin,
T18160	3602-3653	Entity	denotes	globin nucleotides 509–584; βmaj globin nucleotides
T18159	3746-3766	Entity	denotes	4% paraformaldehyde,
T18158	3667-3689	Entity	denotes	mouse Sox6 nucleotides
T18157	4432-4497	Entity	denotes	Pro (Reindeer Graphics, Asheville, North Carolina, United States)
T18156	3602-3608	Protein	denotes	globin
T17715	2947-2972	Regulation	denotes	PCR amplification was run
T17714	2947-2950	Protein	denotes	PCR
T17713	3168-3173	Protein	denotes	GAPDH
T17712	3338-3341	Protein	denotes	PCR
T17711	2586-2594	Protein	denotes	ζ globin
T17710	2895-2918	Protein	denotes	ROX (Bio-Rad, Hercules,
T17709	2774-2780	Protein	denotes	globin
T17708	2272-2283	Protein	denotes	globin mRNA
T17707	2285-2288	Protein	denotes	RNA
T17706	2340-2378	Protein	denotes	cDNA PCR amplification of globin genes
T17705	3471-3476	Protein	denotes	GAPDH
T17704	3092-3099	Protein	denotes	All PCR
T17703	2366-2372	Protein	denotes	globin
T17702	3419-3422	Protein	denotes	SDS
T17055	137-139	Protein	denotes	ɛy
T17054	1906-1908	Protein	denotes	ɛy
T17053	762-812	Protein	denotes	Basic (Promega, Madison, Wisconsin, United States)
T17052	130-157	Entity	denotes	of the ɛy proximal promoter
T17051	422-431	Entity	denotes	site [50]
T17050	196-202	Protein	denotes	globin
T17049	1792-1806	Entity	denotes	the HMG domain
T17048	406-412	Protein	denotes	globin
T17047	1079-1097	Entity	denotes	of the ɛy promoter
T17046	112-115	Protein	denotes	PCR
T17045	52-60	Entity	denotes	promoter
T17044	693-695	Protein	denotes	ɛy
T17043	642-646	Protein	denotes	XhoI
T17042	1730-1734	Protein	denotes	Sox6
T17041	1034-1036	Protein	denotes	ɛy
T17040	907-909	Protein	denotes	ɛy
T17039	1987-1998	Entity	denotes	ɛy promoter
T17038	689-713	Entity	denotes	the ɛy promoter fragment
T17037	1697-1701	Protein	denotes	Sox6
T17036	49-51	Protein	denotes	ɛy
T17035	620-623	Protein	denotes	PCR
T17034	433-443	Entity	denotes	Nucleotide
T17033	400-421	Protein	denotes	the ɛ globin gene cap
T17032	330-375	Entity	denotes	a 3.7-kb EcoRI fragment containing about 2 kb
T17031	1899-1917	Entity	denotes	of the ɛy promoter
T17030	1928-1934	Protein	denotes	by PCR
T17029	689-713	Entity	denotes	the ɛy promoter fragment
T17075	1685-1701	Gene_expression	denotes	overexpress Sox6
T17074	1685-1701	Positive_regulation	denotes	overexpress Sox6
T17073	1723-1780	Positive_regulation	denotes	of the Sox6 overexpression construct (Sox6-ΔHMG-pcDNA3.1)
T17072	1723-1780	Gene_expression	denotes	of the Sox6 overexpression construct (Sox6-ΔHMG-pcDNA3.1)
T17071	189-225	Positive_regulation	denotes	the ɛy globin initiation codon (ATG)
T17070	730-748	Protein	denotes	firefly luciferase
T17069	726-753	Protein	denotes	the firefly luciferase gene
T17068	542-558	Entity	denotes	the longest cDNA
T17067	1568-1599	Entity	denotes	the reverse primer HindIII site
T17066	1145-1157	Entity	denotes	an XhoI site
T17065	1027-1045	Entity	denotes	by the ɛy promoter
T17064	294-310	Protein	denotes	ɛ gene silencing
T17063	404-405	Protein	denotes	ɛ
T17062	1148-1152	Protein	denotes	XhoI
T17061	856-871	Protein	denotes	micro LCR; [31]
T17060	1987-1989	Protein	denotes	ɛy
T17059	1866-1934	Protein	denotes	Sox/Sox6 consensus binding sites of the ɛy promoter were done by PCR
T17058	903-931	Entity	denotes	the ɛy promoter in the above
T17057	193-195	Protein	denotes	ɛy
T17056	651-664	Entity	denotes	HindIII sites
R11921	T17031	T17054	partOf	of the ɛy promoter,ɛy
R11922	T17037	T17074	themeOf	Sox6,overexpress Sox6
R11923	T17037	T17075	themeOf	Sox6,overexpress Sox6
R11924	T17038	T17044	partOf	the ɛy promoter fragment,ɛy
R11925	T17039	T17060	partOf	ɛy promoter,ɛy
R11926	T17042	T17072	themeOf	Sox6,of the Sox6 overexpression construct (Sox6-ΔHMG-pcDNA3.1)
R11927	T17042	T17073	themeOf	Sox6,of the Sox6 overexpression construct (Sox6-ΔHMG-pcDNA3.1)
R11928	T17050	T17071	themeOf	globin,the ɛy globin initiation codon (ATG)
R11929	T17052	T17055	partOf	of the ɛy proximal promoter,ɛy
R11930	T17058	T17040	partOf	the ɛy promoter in the above,ɛy
R11931	T17065	T17041	partOf	by the ɛy promoter,ɛy
R13572	T19089	T19092	themeOf	Sox6,Sox6 overexpression vector
R13573	T19089	T19093	themeOf	Sox6,Sox6 overexpression vector
R13770	T19371	T19378	themeOf	of Sox6,in vitro translation of Sox6
R13933	T19612	T19617	themeOf	c-Myc antibody,c-Myc antibody was purchased from Invitrogen
R15085	T20990	T20997	partOf	the ɛy promoter,ɛy
R15086	T20995	T20996	partOf	of the ɛy promoter,ɛy
R15087	T20999	T21018	themeOf	the DNA protein cross-links,the DNA protein cross-links
R15088	T21003	T21018	locationOf	DNA,the DNA protein cross-links

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T18080	4198-4199	,	denotes	,
T20986	10503-10504	.	denotes	.
T20985	10500-10503	NN	denotes	min
T20984	10498-10499	CD	denotes	5
T20983	10494-10497	IN	denotes	for
T20982	10492-10493	NNP	denotes	C
T20981	10491-10492	CD	denotes	°
T20980	10488-10490	CD	denotes	72
T20979	10485-10487	IN	denotes	at
T20978	10475-10484	NN	denotes	extension
T20977	10469-10474	JJ	denotes	final
T20976	10467-10468	DT	denotes	a
T20975	10462-10466	IN	denotes	with
T20974	10455-10461	VBG	denotes	ending
T20973	10453-10454	,	denotes	,
T20972	10452-10453	PRP	denotes	s
T20971	10449-10451	CD	denotes	45
T20970	10445-10448	IN	denotes	for
T20969	10443-10444	NNP	denotes	C
T20968	10442-10443	NN	denotes	°
T20967	10439-10441	CD	denotes	72
T20966	10435-10438	CC	denotes	and
T20965	10433-10434	,	denotes	,
T20964	10432-10433	PRP	denotes	s
T20963	10429-10431	CD	denotes	30
T20962	10425-10428	IN	denotes	for
T20961	10423-10424	NNP	denotes	C
T20960	10422-10423	NN	denotes	°
T20959	10419-10421	CD	denotes	60
T20958	10417-10418	,	denotes	,
T20957	10416-10417	PRP	denotes	s
T20956	10413-10415	CD	denotes	30
T20955	10409-10412	IN	denotes	for
T20954	10407-10408	NNP	denotes	C
T20953	10406-10407	CD	denotes	°
T20952	10403-10405	CD	denotes	95
T20951	10400-10402	IN	denotes	at
T20950	10393-10399	NNS	denotes	cycles
T20949	10390-10392	CD	denotes	30
T20948	10387-10389	IN	denotes	by
T20947	10378-10386	VBN	denotes	followed
T20946	10374-10377	NN	denotes	min
T20945	10371-10373	CD	denotes	15
T20944	10367-10370	IN	denotes	for
T20943	10365-10366	NNP	denotes	C
T20942	10364-10365	NN	denotes	°
T20941	10361-10363	CD	denotes	95
T20940	10359-10360	:	denotes	:
T20939	10349-10359	NNS	denotes	conditions
T20938	10339-10348	JJ	denotes	following
T20937	10335-10338	DT	denotes	the
T20936	10329-10334	IN	denotes	under
T20935	10319-10328	VBN	denotes	performed
T20934	10315-10318	VBD	denotes	was
T20933	10311-10314	NNP	denotes	PCR
T20932	10309-10310	.	denotes	.
T20931	10308-10309	-RRB-	denotes	)
T20930	10307-10308	NNP	denotes	′
T20929	10286-10307	NNP	denotes	GCTTCACCACCAACCTCTTC3
T20928	10285-10286	NN	denotes	′
T20927	10284-10285	CD	denotes	5
T20926	10282-10283	,	denotes	,
T20925	10275-10282	NNP	denotes	MHB1689
T20924	10271-10274	CC	denotes	and
T20923	10269-10270	:	denotes	;
T20922	10268-10269	NN	denotes	′
T20921	10247-10268	CD	denotes	CGAAGAATAAAAGGCCACCA3
T20920	10246-10247	NN	denotes	′
T20919	10245-10246	CD	denotes	5
T20918	10243-10244	,	denotes	,
T20917	10236-10243	NNP	denotes	MHB1688
T20916	10228-10235	NNS	denotes	primers
T20915	10227-10228	-LRB-	denotes	(
T20914	10218-10226	NN	denotes	promoter
T20913	10215-10217	JJ	denotes	ɛy
T20912	10211-10214	DT	denotes	the
T20911	10208-10210	IN	denotes	of
T20910	10203-10207	CD	denotes	+140
T20909	10200-10202	TO	denotes	to
T20908	10197-10199	CD	denotes	31
T20907	10196-10197	VBP	denotes	−
T20906	10184-10195	NNS	denotes	nucleotides
T20905	10181-10183	TO	denotes	to
T20904	10167-10180	JJ	denotes	corresponding
T20903	10165-10166	,	denotes	,
T20902	10157-10165	NN	denotes	amplicon
T20901	10150-10156	JJ	denotes	172-bp
T20900	10148-10149	DT	denotes	a
T20899	10140-10147	VBD	denotes	yielded
T20898	10136-10139	CC	denotes	and
T20897	10126-10135	VBN	denotes	performed
T20896	10122-10125	VBD	denotes	was
T20895	10113-10121	NN	denotes	promoter
T20894	10110-10112	JJ	denotes	ɛy
T20893	10106-10109	DT	denotes	the
T20892	10103-10105	IN	denotes	of
T20891	10089-10102	NN	denotes	amplification
T20890	10085-10088	NNP	denotes	PCR
T20889	10083-10084	.	denotes	.
T20888	10075-10083	NN	denotes	reaction
T20887	10071-10074	NNP	denotes	PCR
T20886	10067-10070	DT	denotes	the
T20885	10064-10066	IN	denotes	in
T20884	10055-10063	NN	denotes	template
T20883	10053-10054	DT	denotes	a
T20882	10050-10052	IN	denotes	as
T20881	10045-10049	VBN	denotes	used
T20880	10041-10044	VBD	denotes	was
T20879	10037-10040	NN	denotes	DNA
T20878	10018-10036	JJ	denotes	immunoprecipitated
T20877	10015-10017	CC	denotes	or
T20876	10011-10014	NNP	denotes	DNA
T20875	10005-10010	NNP	denotes	Input
T20874	10002-10004	NN	denotes	h.
T20873	10000-10001	CD	denotes	4
T20872	9996-9999	IN	denotes	for
T20871	9994-9995	NNP	denotes	C
T20870	9993-9994	CD	denotes	°
T20869	9990-9992	CD	denotes	65
T20868	9987-9989	IN	denotes	at
T20867	9982-9986	NNP	denotes	NaCl
T20866	9980-9981	NNP	denotes	M
T20865	9978-9979	CD	denotes	5
T20864	9973-9977	IN	denotes	with
T20863	9964-9972	VBN	denotes	reversed
T20862	9959-9963	VBD	denotes	were
T20861	9947-9958	NNS	denotes	cross-links
T20860	9939-9946	NN	denotes	protein
T20859	9935-9938	NNP	denotes	DNA
T20858	9931-9934	DT	denotes	the
T20857	9927-9930	CC	denotes	and
T20856	9925-9926	,	denotes	,
T20855	9919-9925	VBN	denotes	eluted
T20854	9914-9918	VBD	denotes	were
T20853	9904-9913	NNS	denotes	complexes
T20852	9885-9903	JJ	denotes	chromatin-antibody
T20851	9881-9884	DT	denotes	The
T20850	9879-9880	.	denotes	.
T20849	9871-9879	NNS	denotes	controls
T20848	9862-9870	JJ	denotes	negative
T20847	9859-9861	IN	denotes	as
T20846	9854-9858	VBN	denotes	used
T20845	9849-9853	VBD	denotes	were
T20844	9845-9848	CD	denotes	two
T20843	9840-9844	JJ	denotes	last
T20842	9836-9839	DT	denotes	The
T20841	9834-9835	.	denotes	.
T20840	9832-9834	NNP	denotes	Ab
T20839	9824-9831	IN	denotes	without
T20838	9817-9823	NN	denotes	sample
T20837	9811-9816	JJ	denotes	third
T20836	9807-9810	DT	denotes	the
T20835	9803-9806	CC	denotes	and
T20834	9801-9802	,	denotes	,
T20833	9798-9801	NNP	denotes	IgG
T20832	9791-9797	NN	denotes	rabbit
T20831	9784-9790	JJ	denotes	normal
T20830	9779-9783	IN	denotes	with
T20829	9771-9778	VBN	denotes	treated
T20828	9764-9770	JJ	denotes	second
T20827	9762-9763	DT	denotes	a
T20826	9760-9761	,	denotes	,
T20825	9751-9760	JJ	denotes	anti-Sox6
T20824	9746-9750	IN	denotes	with
T20823	9738-9745	VBN	denotes	treated
T20822	9731-9737	NN	denotes	sample
T20821	9727-9730	CD	denotes	one
T20820	9725-9726	:	denotes	:
T20819	9719-9725	NNS	denotes	thirds
T20818	9714-9718	IN	denotes	into
T20817	9708-9713	VBN	denotes	split
T20816	9703-9707	VBD	denotes	were
T20815	9695-9702	NNS	denotes	samples
T20814	9691-9694	DT	denotes	the
T20813	9689-9690	,	denotes	,
T20812	9678-9689	NN	denotes	preclearing
T20811	9668-9677	VBG	denotes	Following
T20810	9666-9667	.	denotes	.
T20809	9665-9666	-RRB-	denotes	)
T20808	9659-9665	NNPS	denotes	States
T20807	9652-9658	NNP	denotes	United
T20806	9650-9651	,	denotes	,
T20805	9644-9650	NNP	denotes	Jersey
T20804	9640-9643	NNP	denotes	New
T20689	9047-9049	IN	denotes	in
T20688	9045-9046	,	denotes	,
T20687	9044-9045	NNP	denotes	]
T20686	9042-9044	CD	denotes	53
T20685	9041-9042	NNP	denotes	[
T20684	9033-9040	NNP	denotes	Nouzova
T20683	9030-9032	IN	denotes	by
T20682	9020-9029	VBN	denotes	described
T20681	9017-9019	IN	denotes	As
T20680	9015-9016	.	denotes	.
T20679	9010-9015	NN	denotes	assay
T20678	9005-9009	NN	denotes	ChIP
T20803	9638-9639	,	denotes	,
T20802	9628-9638	NNP	denotes	Piscataway
T20801	9626-9627	,	denotes	,
T20800	9615-9626	NNP	denotes	Biosciences
T20799	9606-9614	NNP	denotes	Amersham
T20798	9605-9606	-LRB-	denotes	(
T20797	9593-9604	JJ	denotes	A-Sepharose
T20796	9585-9592	NN	denotes	protein
T20795	9580-9584	IN	denotes	with
T20794	9569-9579	VBN	denotes	precleared
T20793	9565-9568	VBD	denotes	was
T20792	9558-9564	NN	denotes	sample
T20791	9548-9557	VBG	denotes	remaining
T20790	9544-9547	DT	denotes	the
T20789	9540-9543	CC	denotes	and
T20788	9538-9539	,	denotes	,
T20787	9531-9538	NN	denotes	control
T20786	9525-9530	NN	denotes	input
T20785	9521-9524	IN	denotes	for
T20784	9515-9520	RB	denotes	aside
T20783	9511-9514	VBN	denotes	set
T20782	9507-9510	VBD	denotes	was
T20781	9500-9506	NN	denotes	sample
T20780	9496-9499	DT	denotes	the
T20779	9493-9495	IN	denotes	of
T20778	9483-9492	NN	denotes	One-tenth
T20777	9481-9482	.	denotes	.
T20776	9479-9481	NN	denotes	bp
T20775	9475-9478	CD	denotes	600
T20774	9471-9474	CC	denotes	and
T20773	9467-9470	CD	denotes	200
T20772	9464-9466	TO	denotes	to
T20771	9454-9463	VBN	denotes	sonicated
T20770	9449-9453	VBD	denotes	were
T20769	9439-9448	NNS	denotes	complexes
T20768	9427-9438	JJ	denotes	DNA-protein
T20767	9425-9426	.	denotes	.
T20766	9422-9425	NN	denotes	min
T20765	9419-9421	CD	denotes	10
T20764	9415-9418	IN	denotes	for
T20763	9411-9414	NN	denotes	ice
T20762	9408-9410	IN	denotes	on
T20761	9398-9407	VBD	denotes	incubated
T20760	9394-9397	CC	denotes	and
T20759	9392-9393	,	denotes	,
T20758	9382-9392	NNS	denotes	inhibitors
T20757	9373-9381	NN	denotes	protease
T20756	9362-9372	VBG	denotes	containing
T20755	9355-9361	NN	denotes	buffer
T20754	9349-9354	NN	denotes	lysis
T20753	9345-9348	NNP	denotes	SDS
T20752	9343-9344	DT	denotes	a
T20751	9340-9342	IN	denotes	in
T20750	9328-9339	VBD	denotes	resuspended
T20749	9326-9327	,	denotes	,
T20748	9325-9326	NNP	denotes	C
T20747	9324-9325	CD	denotes	°
T20746	9322-9323	CD	denotes	4
T20745	9319-9321	IN	denotes	at
T20744	9304-9318	NN	denotes	centrifugation
T20743	9301-9303	IN	denotes	by
T20742	9291-9300	VBN	denotes	collected
T20741	9289-9290	,	denotes	,
T20740	9279-9289	NNS	denotes	inhibitors
T20739	9270-9278	NN	denotes	protease
T20738	9259-9269	VBG	denotes	containing
T20737	9254-9258	NNP	denotes	EDTA
T20736	9252-9253	NN	denotes	%
T20735	9249-9252	CD	denotes	0.1
T20734	9244-9248	IN	denotes	with
T20733	9235-9243	NN	denotes	solution
T20732	9230-9234	NN	denotes	salt
T20731	9221-9229	JJ	denotes	balanced
T20730	9219-9220	POS	denotes	'
T20729	9214-9219	NNP	denotes	Hanks
T20728	9212-9213	CD	denotes	×
T20727	9211-9212	CD	denotes	1
T20726	9202-9210	JJ	denotes	ice-cold
T20725	9199-9201	IN	denotes	in
T20724	9192-9198	VBD	denotes	rinsed
T20723	9190-9191	,	denotes	,
T20722	9189-9190	NNP	denotes	C
T20721	9188-9189	CD	denotes	°
T20720	9185-9187	CD	denotes	37
T20719	9182-9184	IN	denotes	at
T20718	9178-9181	NN	denotes	min
T20717	9175-9177	CD	denotes	10
T20716	9171-9174	IN	denotes	for
T20715	9158-9170	NN	denotes	formaldehyde
T20714	9156-9157	NN	denotes	%
T20713	9155-9156	CD	denotes	1
T20712	9150-9154	IN	denotes	with
T20711	9142-9149	VBN	denotes	treated
T20710	9137-9141	VBD	denotes	were
T20709	9132-9136	NNS	denotes	mice
T20708	9129-9131	NNP	denotes	WT
T20707	9120-9128	JJ	denotes	15.5-dpc
T20706	9114-9119	CD	denotes	three
T20705	9109-9113	IN	denotes	from
T20704	9103-9108	NNS	denotes	cells
T20703	9097-9102	NN	denotes	liver
T20702	9091-9096	JJ	denotes	fetal
T20701	9088-9090	CC	denotes	or
T20700	9086-9087	-RRB-	denotes	)
T20699	9083-9086	CD	denotes	107
T20698	9081-9082	CD	denotes	×
T20697	9079-9080	CD	denotes	4
T20696	9078-9079	-LRB-	denotes	(
T20695	9072-9077	NNS	denotes	cells
T20694	9068-9071	NNP	denotes	MEL
T20693	9063-9067	IN	denotes	from
T20692	9057-9062	NNS	denotes	Cells
T20691	9055-9056	:	denotes	:
T20690	9050-9055	NN	denotes	brief
T20273	9002-9003	.	denotes	.
T20272	9001-9002	-RRB-	denotes	)
T20271	8994-9001	NNP	denotes	MHB1650
T20270	8993-8994	-LRB-	denotes	(
T20269	8991-8992	NNP	denotes	′
T20268	8954-8991	NNP	denotes	AATGCAGtgccAAGGGTCAGAACATTGTCTGCGAAG3
T20267	8953-8954	NN	denotes	′
T20266	8952-8953	CD	denotes	5
T20265	8950-8951	:	denotes	:
T20264	8949-8950	-RRB-	denotes	)
T20263	8947-8949	NN	denotes	M3
T20262	8946-8947	-LRB-	denotes	(
T20261	8944-8945	CD	denotes	3
T20260	8938-8943	NN	denotes	probe
T20259	8931-8937	JJ	denotes	mutant
T20258	8927-8930	IN	denotes	for
T20226	8780-8783	IN	denotes	for
T20225	8778-8779	:	denotes	;
T20224	8777-8778	-RRB-	denotes	)
T20223	8770-8777	NNP	denotes	MHB1556
T20222	8769-8770	-LRB-	denotes	(
T20221	8767-8768	NNP	denotes	′
T20220	8730-8767	NNP	denotes	AATGCAGAACAAAGGGTCAGAACATTGTCTGCGAAG3
T20219	8729-8730	CD	denotes	′
T20218	8728-8729	CD	denotes	5
T20217	8726-8727	:	denotes	:
T20216	8721-8726	NN	denotes	probe
T20215	8718-8720	NNP	denotes	WT
T20214	8712-8717	JJ	denotes	36-bp
T20213	8708-8711	DT	denotes	the
T20212	8704-8707	IN	denotes	For
T20211	8702-8703	:	denotes	:
T20210	8701-8702	-RRB-	denotes	)
T20209	8695-8701	VBN	denotes	listed
T20208	8691-8694	VBP	denotes	are
T20207	8684-8690	NNS	denotes	oligos
T20206	8676-8683	RB	denotes	forward
T20205	8671-8675	RB	denotes	only
T20204	8670-8671	-LRB-	denotes	(
T20203	8662-8669	VBZ	denotes	follows
T20202	8659-8661	IN	denotes	as
T20201	8655-8658	VBP	denotes	are
T20200	8638-8654	NNS	denotes	oligonucleotides
T20199	8634-8637	DT	denotes	the
T20198	8631-8633	IN	denotes	of
T20197	8621-8630	NNS	denotes	sequences
T20196	8617-8620	NNP	denotes	DNA
T20195	8613-8616	DT	denotes	The
T20194	8611-8612	.	denotes	.
T20193	8610-8611	-RRB-	denotes	)
T20192	8605-8610	IN	denotes	above
T20191	8602-8604	RB	denotes	as
T20190	8592-8601	VBN	denotes	described
T20189	8591-8592	-LRB-	denotes	(
T20188	8580-8590	NNS	denotes	antibodies
T20187	8575-8579	NNP	denotes	Sox6
T20186	8571-8574	CC	denotes	and
T20185	8569-8570	,	denotes	,
T20184	8567-8569	NNP	denotes	HA
T20183	8565-8566	,	denotes	,
T20182	8560-8565	NNP	denotes	c-Myc
T20181	8551-8559	VBD	denotes	included
T20180	8542-8550	NNS	denotes	analyses
T20179	8531-8541	NN	denotes	supershift
T20178	8527-8530	IN	denotes	for
T20177	8522-8526	VBD	denotes	used
T20176	8511-8521	NNS	denotes	Antibodies
T20175	8509-8510	.	denotes	.
T20174	8494-8509	NN	denotes	autoradiography
T20173	8491-8493	TO	denotes	to
T20172	8485-8490	RB	denotes	prior
T20171	8479-8484	VBN	denotes	dried
T20170	8474-8478	VBD	denotes	were
T20169	8469-8473	NNS	denotes	gels
T20168	8465-8468	DT	denotes	the
T20167	8461-8464	CC	denotes	and
T20166	8459-8460	,	denotes	,
T20165	8448-8459	NN	denotes	temperature
T20164	8443-8447	NN	denotes	room
T20163	8440-8442	IN	denotes	at
T20162	8438-8439	NN	denotes	h
T20161	8436-8437	CD	denotes	8
T20160	8434-8435	CD	denotes	4
T20159	8430-8433	IN	denotes	for
T20158	8425-8429	NN	denotes	mAmp
T20157	8422-8424	CD	denotes	19
T20156	8413-8421	JJ	denotes	constant
T20155	8411-8412	DT	denotes	a
T20154	8408-8410	IN	denotes	at
T20153	8398-8407	VBN	denotes	performed
T20152	8394-8397	VBD	denotes	was
T20151	8378-8393	NNP	denotes	Electrophoresis
T20150	8376-8377	.	denotes	.
T20149	8373-8376	NN	denotes	gel
T20148	8358-8372	NN	denotes	polyacrylamide
T20147	8356-8357	-RRB-	denotes	)
T20146	8353-8356	FW	denotes	w/v
T20145	8352-8353	-LRB-	denotes	(
T20144	8350-8351	NN	denotes	%
T20143	8349-8350	CD	denotes	6
T20142	8346-8348	CC	denotes	or
T20141	8344-8345	NN	denotes	%
T20140	8343-8344	CD	denotes	4
T20139	8341-8342	DT	denotes	a
T20138	8338-8340	IN	denotes	on
T20137	8331-8337	VBN	denotes	loaded
T20136	8327-8330	CC	denotes	and
T20135	8315-8326	NN	denotes	temperature
T20134	8310-8314	NN	denotes	room
T20133	8307-8309	IN	denotes	at
T20132	8303-8306	NN	denotes	min
T20131	8300-8302	CD	denotes	60
T20130	8297-8299	CC	denotes	or
T20129	8293-8296	NN	denotes	min
T20128	8290-8292	CD	denotes	30
T20127	8286-8289	IN	denotes	for
T20126	8276-8285	VBN	denotes	incubated
T20125	8271-8275	VBD	denotes	were
T20124	8263-8270	NNS	denotes	samples
T20123	8259-8262	DT	denotes	the
T20122	8257-8258	,	denotes	,
T20121	8252-8257	NN	denotes	probe
T20120	8239-8251	JJ	denotes	radiolabeled
T20119	8235-8238	DT	denotes	the
T20118	8232-8234	IN	denotes	of
T20117	8223-8231	NN	denotes	addition
T20116	8213-8222	VBG	denotes	Following
T20115	8211-8212	.	denotes	.
T20114	8206-8211	NN	denotes	probe
T20113	8193-8205	JJ	denotes	radiolabeled
T20112	8190-8192	IN	denotes	of
T20111	8181-8189	NN	denotes	addition
T20110	8178-8180	TO	denotes	to
T20109	8172-8177	RB	denotes	prior
T20108	8168-8171	NN	denotes	min
T20107	8165-8167	CD	denotes	60
T20106	8162-8164	TO	denotes	to
T20105	8158-8161	NN	denotes	min
T20104	8155-8157	CD	denotes	30
T20103	8149-8154	VBN	denotes	added
T20102	8145-8148	VBD	denotes	was
T20101	8143-8144	-RRB-	denotes	)
T20100	8141-8143	NN	denotes	μl
T20099	8139-8140	CD	denotes	3
T20098	8138-8139	-LRB-	denotes	(
T20097	8129-8137	NN	denotes	antibody
T20096	8126-8128	CC	denotes	or
T20095	8124-8125	-RRB-	denotes	)
T20094	8118-8124	NN	denotes	excess
T20093	8112-8117	NN	denotes	molar
T20092	8103-8111	JJ	denotes	200-fold
T20091	8102-8103	-LRB-	denotes	(
T20090	8091-8101	NN	denotes	competitor
T20089	8075-8090	NN	denotes	oligonucleotide
T20088	8064-8074	JJ	denotes	unlabelled
T20087	8054-8063	VBN	denotes	indicated
T20086	8050-8053	DT	denotes	the
T20085	8048-8049	,	denotes	,
T20084	8042-8048	NNS	denotes	assays
T20083	8031-8041	NN	denotes	supershift
T20082	8028-8030	CC	denotes	or
T20081	8016-8027	NN	denotes	competition
T20080	8012-8015	IN	denotes	For
T20079	8010-8011	.	denotes	.
T20078	8005-8010	NNP	denotes	MgCl2
T20077	8002-8004	NNP	denotes	mM
T20076	7998-8001	CD	denotes	0.6
T20075	7996-7997	,	denotes	,
T20074	7993-7996	NNP	denotes	DTT
T20073	7990-7992	NNP	denotes	mM
T20072	7985-7989	CD	denotes	0.25
T20071	7983-7984	,	denotes	,
T20070	7979-7983	NNP	denotes	EDTA
T20069	7976-7978	NNP	denotes	mM
T20068	7972-7975	CD	denotes	0.1
T20067	7970-7971	,	denotes	,
T20066	7969-7970	-RRB-	denotes	)
T20065	7968-7969	CD	denotes	7
T20064	7965-7967	NN	denotes	pH
T20063	7964-7965	-LRB-	denotes	(
T20062	7958-7963	NNS	denotes	HEPES
T20061	7955-7957	NN	denotes	mM
T20060	7952-7954	CD	denotes	10
T20059	7950-7951	,	denotes	,
T20058	7949-7950	-RRB-	denotes	)
T20057	7944-7949	JJ	denotes	dG-dC
T20056	7943-7944	-LRB-	denotes	(
T20055	7938-7942	NN	denotes	poly
T20054	7935-7937	CC	denotes	or
T20053	7933-7934	-RRB-	denotes	)
T20052	7928-7933	JJ	denotes	dI-dC
T20051	7927-7928	-LRB-	denotes	(
T20050	7922-7926	NN	denotes	poly
T20049	7919-7921	NN	denotes	μl
T20048	7918-7919	NN	denotes	/
T20047	7916-7918	NN	denotes	ng
T20046	7913-7915	CD	denotes	50
T20045	7911-7912	,	denotes	,
T20044	7908-7911	NNP	denotes	BSA
T20043	7905-7907	NN	denotes	μl
T20042	7904-7905	NN	denotes	/
T20041	7902-7904	NN	denotes	ng
T20040	7898-7901	CD	denotes	200
T20039	7896-7897	,	denotes	,
T20038	7888-7896	NN	denotes	glycerol
T20037	7886-7887	NN	denotes	%
T20036	7884-7886	CD	denotes	10
T20035	7882-7883	,	denotes	,
T20034	7878-7882	NNP	denotes	NaCl
T20033	7875-7877	NNP	denotes	mM
T20032	7871-7874	CD	denotes	100
T20031	7869-7870	:	denotes	:
T20030	7863-7869	NN	denotes	buffer
T20029	7855-7862	JJ	denotes	binding
T20028	7852-7854	IN	denotes	in
T20027	7845-7851	NN	denotes	lysate
T20026	7832-7844	NN	denotes	reticulocyte
T20025	7828-7831	DT	denotes	the
T20024	7823-7827	IN	denotes	with
T20023	7817-7822	IN	denotes	along
T20022	7812-7816	NNS	denotes	Sox6
T20021	7795-7811	JJ	denotes	vitro-translated
T20020	7792-7794	IN	denotes	in
T20019	7789-7791	IN	denotes	of
T20018	7786-7788	NN	denotes	μl
T20017	7784-7785	CD	denotes	3
T20016	7781-7783	CC	denotes	or
T20015	7775-7780	NNS	denotes	cells
T20014	7771-7774	NNP	denotes	MEL
T20013	7766-7770	IN	denotes	from
T20012	7757-7765	NNS	denotes	proteins
T20011	7749-7756	JJ	denotes	nuclear
T19990	7639-7643	VBD	denotes	were
T19989	7622-7638	NNS	denotes	oligonucleotides
T19988	7608-7621	JJ	denotes	complementary
T19987	7592-7607	JJ	denotes	Single-stranded
T19986	7590-7591	.	denotes	.
T19985	7586-7590	NNP	denotes	EMSA
T20257	8925-8926	:	denotes	;
T20256	8924-8925	-RRB-	denotes	)
T20255	8917-8924	NNP	denotes	MHB1648
T20254	8916-8917	-LRB-	denotes	(
T20253	8914-8915	NNP	denotes	′
T20252	8877-8914	NNP	denotes	AATGCAGAACAAAGGGTCAGAtgagTGTCTGCGAAG3
T20251	8876-8877	NN	denotes	′
T20250	8875-8876	CD	denotes	5
T20249	8873-8874	:	denotes	:
T20248	8872-8873	-RRB-	denotes	)
T20247	8870-8872	NN	denotes	M2
T20246	8869-8870	-LRB-	denotes	(
T20245	8867-8868	CD	denotes	2
T20244	8861-8866	NN	denotes	probe
T20243	8854-8860	JJ	denotes	mutant
T20242	8850-8853	IN	denotes	for
T20241	8848-8849	:	denotes	;
T20240	8847-8848	-RRB-	denotes	)
T20239	8840-8847	NNP	denotes	MHB1644
T20238	8839-8840	-LRB-	denotes	(
T20237	8837-8838	NNP	denotes	′
T20236	8807-8837	NNP	denotes	AACAAAGGGTCAGAACATTGTCTGCGAAG3
T20235	8806-8807	CD	denotes	′
T20234	8805-8806	CD	denotes	5
T20233	8803-8804	:	denotes	:
T20232	8802-8803	-RRB-	denotes	)
T20231	8800-8802	NN	denotes	M1
T20230	8799-8800	-LRB-	denotes	(
T20229	8797-8798	CD	denotes	1
T20228	8791-8796	NN	denotes	probe
T20227	8784-8790	JJ	denotes	mutant
T19604	7583-7584	.	denotes	.
T19603	7582-7583	-RRB-	denotes	)
T19602	7576-7582	NNPS	denotes	States
T19601	7569-7575	NNP	denotes	United
T19600	7567-7568	,	denotes	,
T19599	7563-7567	NNP	denotes	York
T19598	7559-7562	NNP	denotes	New
T19597	7557-7558	,	denotes	,
T19596	7551-7557	NNP	denotes	Placid
T19595	7546-7550	NNP	denotes	Lake
T19594	7545-7546	-LRB-	denotes	(
T19593	7531-7544	NNP	denotes	Biotechnology
T19592	7523-7530	NNP	denotes	Upstate
T19591	7518-7522	IN	denotes	from
T20010	7746-7748	IN	denotes	of
T20009	7743-7745	NN	denotes	μg
T20008	7741-7742	CD	denotes	5
T20007	7736-7740	IN	denotes	with
T20006	7726-7735	VBN	denotes	performed
T20005	7722-7725	VBD	denotes	was
T20004	7717-7721	NNP	denotes	EMSA
T20003	7715-7716	.	denotes	.
T20002	7709-7715	NN	denotes	kinase
T20001	7694-7708	NN	denotes	polynucleotide
T20000	7691-7693	CD	denotes	T4
T19999	7686-7690	IN	denotes	with
T19998	7682-7685	NNP	denotes	ATP
T19997	7680-7681	NNP	denotes	]
T19996	7675-7680	NNP	denotes	γ-32P
T19995	7674-7675	NNP	denotes	[
T19994	7669-7673	IN	denotes	with
T19993	7657-7668	JJ	denotes	end-labeled
T19992	7653-7656	CC	denotes	and
T19991	7644-7652	JJ	denotes	annealed
T19590	7509-7517	VBN	denotes	obtained
T19589	7505-7508	VBD	denotes	was
T19588	7496-7504	NN	denotes	antibody
T19587	7492-7495	NNP	denotes	IgG
T19586	7485-7491	NN	denotes	rabbit
T19585	7478-7484	JJ	denotes	Normal
T19584	7476-7477	.	denotes	.
T19583	7466-7476	NNP	denotes	Invitrogen
T19582	7461-7465	IN	denotes	from
T19581	7451-7460	VBN	denotes	purchased
T19580	7447-7450	VBD	denotes	was
T19579	7438-7446	NN	denotes	antibody
T19578	7432-7437	JJ	denotes	c-Myc
T19577	7430-7431	.	denotes	.
T19576	7423-7430	NNS	denotes	results
T19575	7415-7422	JJ	denotes	similar
T19574	7405-7414	VBD	denotes	generated
T19573	7394-7404	NNS	denotes	antibodies
T19572	7389-7393	JJ	denotes	Sox6
T19571	7385-7388	DT	denotes	All
T19570	7383-7384	.	denotes	.
T19569	7382-7383	-RRB-	denotes	)
T19568	7376-7382	NNPS	denotes	States
T19567	7369-7375	NNP	denotes	United
T19566	7367-7368	,	denotes	,
T19565	7357-7367	NNP	denotes	California
T19564	7355-7356	,	denotes	,
T19563	7351-7355	NNP	denotes	Cruz
T19562	7345-7350	NNP	denotes	Santa
T19561	7343-7344	,	denotes	,
T19560	7330-7343	NNP	denotes	Biotechnology
T19559	7325-7329	NNP	denotes	Cruz
T19558	7319-7324	NNP	denotes	Santa
T19557	7317-7318	,	denotes	,
T19556	7316-7317	NNP	denotes	X
T19555	7307-7315	JJ	denotes	sc-17332
T19554	7305-7306	.	denotes	.
T19553	7303-7305	UH	denotes	No
T19552	7295-7302	NN	denotes	Catalog
T19551	7294-7295	-LRB-	denotes	(
T19550	7285-7293	VBN	denotes	obtained
T19549	7272-7284	RB	denotes	commercially
T19548	7269-7271	CC	denotes	or
T19547	7267-7268	-RRB-	denotes	)
T19546	7266-7267	NNP	denotes	]
T19545	7265-7266	CD	denotes	7
T19544	7264-7265	NNP	denotes	[
T19543	7257-7263	NNP	denotes	France
T19542	7255-7256	,	denotes	,
T19541	7248-7255	NNP	denotes	Pasteur
T19540	7242-7247	NNP	denotes	Louis
T19539	7231-7241	NNP	denotes	Université
T19538	7230-7231	-LRB-	denotes	(
T19537	7224-7229	NNP	denotes	Lalli
T19536	7219-7223	NNP	denotes	Enzo
T19535	7215-7218	NNP	denotes	Dr.
T19534	7212-7214	IN	denotes	by
T19533	7203-7211	VBN	denotes	provided
T19532	7196-7202	RB	denotes	kindly
T19531	7189-7195	RB	denotes	either
T19530	7184-7188	VBD	denotes	were
T19529	7178-7183	NN	denotes	study
T19528	7173-7177	DT	denotes	this
T19527	7170-7172	IN	denotes	in
T19526	7165-7169	VBN	denotes	used
T19525	7154-7164	NNS	denotes	antibodies
T19524	7149-7153	JJ	denotes	Sox6
T19523	7147-7148	.	denotes	.
T19522	7137-7147	NNS	denotes	Antibodies
T19273	6651-6662	NN	denotes	translation
T19272	6645-6650	NN	denotes	vitro
T19271	6642-6644	IN	denotes	in
T19270	6638-6641	CC	denotes	and
T19269	6630-6637	VB	denotes	extract
T19268	6622-6629	NN	denotes	protein
T19267	6614-6621	NNP	denotes	Nuclear
T19074	6611-6612	.	denotes	.
T19073	6601-6611	NN	denotes	efficiency
T19072	6588-6600	NN	denotes	transfection
T19071	6584-6587	IN	denotes	for
T19070	6576-6583	NN	denotes	control
T19069	6574-6575	DT	denotes	a
T19068	6571-6573	IN	denotes	as
T19067	6566-6570	VBN	denotes	used
T19066	6562-6565	VBD	denotes	was
T19065	6560-6561	-RRB-	denotes	)
T19064	6553-6560	NNP	denotes	Promega
T19063	6552-6553	-LRB-	denotes	(
T19062	6547-6551	JJ	denotes	15ng
T19061	6539-6546	JJ	denotes	pRL-CMV
T19060	6537-6538	.	denotes	.
T19059	6536-6537	-RRB-	denotes	)
T19058	6534-6536	NN	denotes	ng
T19051	6513-6515	NN	denotes	ng
T19050	6509-6512	CD	denotes	200
T19049	6504-6508	VBD	denotes	used
T19036	6455-6461	NN	denotes	vector
T19035	6440-6454	NN	denotes	overexpression
T19034	6435-6439	JJ	denotes	Sox6
T19033	6432-6434	CC	denotes	or
T19032	6425-6431	NN	denotes	vector
T19031	6419-6424	JJ	denotes	empty
T19030	6412-6418	DT	denotes	either
T19029	6407-6411	IN	denotes	with
T19028	6401-6406	IN	denotes	along
T19027	6399-6400	-RRB-	denotes	)
T19026	6397-6399	NN	denotes	ng
T19025	6393-6396	CD	denotes	500
T19024	6392-6393	-LRB-	denotes	(
T19023	6381-6391	NNS	denotes	constructs
T19022	6372-6380	NN	denotes	reporter
T19021	6363-6371	NN	denotes	promoter
T19020	6360-6362	JJ	denotes	ɛy
T19019	6355-6359	IN	denotes	with
T19018	6343-6354	VBN	denotes	transfected
T19017	6338-6342	VBD	denotes	were
T19016	6332-6337	NNS	denotes	Cells
T19015	6330-6331	.	denotes	.
T19014	6329-6330	-RRB-	denotes	)
T19013	6323-6329	NNPS	denotes	States
T19012	6316-6322	NNP	denotes	United
T19011	6314-6315	,	denotes	,
T19010	6307-6314	NNP	denotes	Indiana
T19009	6305-6306	,	denotes	,
T19008	6293-6305	NNP	denotes	Indianapolis
T19007	6291-6292	,	denotes	,
T19006	6286-6291	NNP	denotes	Roche
T19005	6285-6286	-LRB-	denotes	(
T19004	6277-6284	NNP	denotes	FuGENE6
T19003	6274-6276	IN	denotes	by
T19002	6265-6273	NNS	denotes	plasmids
T19001	6260-6264	IN	denotes	with
T19000	6248-6259	VBN	denotes	transfected
T18999	6243-6247	VBD	denotes	were
T18998	6236-6242	NN	denotes	growth
T18997	6233-6235	IN	denotes	of
T18996	6227-6232	NN	denotes	phase
T18995	6223-6226	VB	denotes	log
T18994	6220-6222	IN	denotes	in
T18993	6218-6219	-RRB-	denotes	)
T18992	6215-6218	CD	denotes	105
T18991	6213-6214	CD	denotes	×
T18990	6211-6212	CD	denotes	4
T18989	6210-6211	-LRB-	denotes	(
T18988	6204-6209	NNS	denotes	cells
T18987	6198-6203	CD	denotes	GM979
T18986	6196-6197	.	denotes	.
T18985	6195-6196	-RRB-	denotes	)
T18984	6190-6195	NN	denotes	serum
T18983	6186-6189	DT	denotes	the
T18982	6173-6185	VBG	denotes	inactivating
T18981	6168-6172	NN	denotes	heat
T18980	6160-6167	IN	denotes	without
T18979	6159-6160	-LRB-	denotes	(
T18978	6153-6158	IN	denotes	above
T18977	6150-6152	IN	denotes	as
T18976	6137-6149	VBD	denotes	supplemented
T18975	6132-6136	NNP	denotes	DMEM
T18974	6129-6131	IN	denotes	in
T18973	6120-6128	VBN	denotes	cultured
T18972	6115-6119	VBD	denotes	were
T18971	6109-6114	NNS	denotes	cells
T18970	6105-6108	NNP	denotes	MEL
T18969	6103-6104	.	denotes	.
T18968	6102-6103	-RRB-	denotes	)
T18967	6100-6102	NNP	denotes	mM
T18966	6098-6099	CD	denotes	2
T18965	6097-6098	-LRB-	denotes	(
T18964	6085-6096	NNP	denotes	L-glutamine
T18963	6081-6084	CC	denotes	and
T18962	6079-6080	,	denotes	,
T18961	6078-6079	-RRB-	denotes	)
T18960	6076-6078	NN	denotes	ml
T18959	6075-6076	NN	denotes	/
T18958	6073-6075	NN	denotes	μg
T18957	6069-6072	CD	denotes	100
T18956	6056-6068	NNP	denotes	streptomycin
T18955	6054-6055	,	denotes	,
T18954	6053-6054	-RRB-	denotes	)
T18953	6045-6053	NN	denotes	units/ml
T18952	6041-6044	CD	denotes	100
T18951	6040-6041	-LRB-	denotes	(
T18950	6029-6039	NN	denotes	penicillin
T18949	6027-6028	,	denotes	,
T18948	6026-6027	-RRB-	denotes	)
T18947	6020-6026	NNPS	denotes	States
T18946	6013-6019	NNP	denotes	United
T18945	6011-6012	,	denotes	,
T18944	6001-6011	NNP	denotes	California
T18943	5999-6000	,	denotes	,
T18942	5991-5999	NNP	denotes	Carlsbad
T18941	5989-5990	,	denotes	,
T18940	5980-5989	NNP	denotes	Ivitrogen
T18939	5979-5980	-LRB-	denotes	(
T18938	5973-5978	NN	denotes	serum
T18937	5968-5972	NN	denotes	calf
T19363	7134-7135	.	denotes	.
T19362	7127-7134	NN	denotes	control
T19361	7118-7126	JJ	denotes	negative
T19360	7116-7117	DT	denotes	a
T19359	7113-7115	IN	denotes	as
T19358	7102-7112	VBN	denotes	translated
T19357	7097-7101	RB	denotes	also
T19356	7093-7096	VBD	denotes	was
T19355	7084-7092	NN	denotes	sequence
T19354	7077-7083	NN	denotes	coding
T19353	7072-7076	JJ	denotes	Sox6
T19352	7068-7071	DT	denotes	the
T19351	7060-7067	IN	denotes	without
T19350	7053-7059	NN	denotes	vector
T19349	7051-7052	DT	denotes	A
T19348	7049-7050	.	denotes	.
T19347	7048-7049	-RRB-	denotes	)
T19346	7041-7048	NNP	denotes	Promega
T19345	7039-7040	,	denotes	,
T19344	7032-7039	NNPS	denotes	Systems
T19343	7006-7031	NNP	denotes	Transcription/Translation
T19342	6998-7005	NNP	denotes	Coupled
T19341	6992-6997	NNP	denotes	Quick
T19340	6990-6991	CD	denotes	®
T19339	6987-6990	NNP	denotes	TNT
T19338	6986-6987	-LRB-	denotes	(
T19337	6979-6985	NN	denotes	system
T19336	6965-6978	JJ	denotes	translational
T19335	6959-6964	NN	denotes	vitro
T19334	6956-6958	IN	denotes	in
T19333	6950-6955	VBN	denotes	based
T19332	6943-6949	NN	denotes	lysate
T19331	6930-6942	NN	denotes	reticulocyte
T19330	6928-6929	DT	denotes	a
T19329	6925-6927	IN	denotes	in
T19328	6915-6924	VBN	denotes	performed
T19327	6911-6914	VBD	denotes	was
T19314	6854-6859	JJ	denotes	c-Myc
T19313	6849-6853	IN	denotes	with
T19312	6842-6848	VBN	denotes	tagged
T19311	6840-6841	,	denotes	,
T19310	6834-6840	NN	denotes	vector
T19309	6823-6833	NN	denotes	expression
T19308	6811-6822	NN	denotes	translation
T19307	6805-6810	NN	denotes	vitro
T19306	6802-6804	IN	denotes	in
T19305	6797-6801	NNP	denotes	Sox6
T19304	6793-6796	DT	denotes	The
T19303	6791-6792	.	denotes	.
T19302	6790-6791	-RRB-	denotes	)
T19301	6784-6790	NNPS	denotes	States
T19300	6777-6783	NNP	denotes	United
T19299	6775-6776	,	denotes	,
T19298	6765-6775	NNP	denotes	California
T19297	6763-6764	,	denotes	,
T19296	6755-6763	NNP	denotes	Carlsbad
T19295	6753-6754	,	denotes	,
T19294	6748-6753	NN	denotes	Motif
T19293	6741-6747	JJ	denotes	Active
T19292	6740-6741	-LRB-	denotes	(
T19291	6736-6739	NN	denotes	kit
T19290	6734-6735	DT	denotes	a
T19289	6728-6733	VBG	denotes	using
T19288	6726-6727	-RRB-	denotes	)
T19287	6723-6726	CD	denotes	107
T19286	6721-6722	CD	denotes	×
T19285	6719-6720	CD	denotes	2
T19284	6718-6719	-LRB-	denotes	(
T19283	6712-6717	NNS	denotes	cells
T19282	6708-6711	NNP	denotes	MEL
T19281	6703-6707	IN	denotes	from
T19280	6694-6702	VBN	denotes	prepared
T19279	6689-6693	VBD	denotes	were
T19278	6680-6688	NNS	denotes	extracts
T19277	6672-6679	NNP	denotes	Nuclear
T19276	6670-6671	.	denotes	.
T19275	6666-6670	NNP	denotes	Sox6
T19274	6663-6665	IN	denotes	of
T18936	5962-5967	JJ	denotes	fetal
T18935	5960-5961	NN	denotes	%
T18934	5958-5960	CD	denotes	10
T18933	5941-5957	JJ	denotes	heat-inactivated
T18932	5936-5940	IN	denotes	with
T18931	5923-5935	VBD	denotes	supplemented
T18930	5911-5922	NNP	denotes	L-glutamine
T18929	5908-5910	NNP	denotes	mM
T18928	5906-5907	CD	denotes	2
T18927	5901-5905	IN	denotes	with
T18926	5897-5900	CD	denotes	F12
T18925	5894-5896	POS	denotes	's
T18924	5891-5894	NNP	denotes	Ham
T18923	5888-5890	IN	denotes	in
T18922	5879-5887	VBN	denotes	cultured
T18921	5874-5878	VBD	denotes	were
T18920	5872-5873	-RRB-	denotes	)
T18919	5866-5872	NNPS	denotes	States
T18918	5859-5865	NNP	denotes	United
T18917	5857-5858	,	denotes	,
T18916	5851-5857	NNP	denotes	Jersey
T18915	5847-5850	NNP	denotes	New
T18914	5845-5846	,	denotes	,
T18913	5839-5845	NNP	denotes	Camden
T18912	5837-5838	,	denotes	,
T18911	5825-5837	NNPS	denotes	Repositories
T18910	5820-5824	NNP	denotes	Cell
T18909	5812-5819	NNP	denotes	Coriell
T18908	5811-5812	-LRB-	denotes	(
T18907	5805-5810	NNS	denotes	cells
T18906	5799-5804	CD	denotes	GM979
T18905	5797-5798	.	denotes	.
T18904	5785-5797	NN	denotes	transfection
T18903	5781-5784	CC	denotes	and
T18902	5773-5780	NN	denotes	culture
T18901	5768-5772	NNP	denotes	Cell
T18684	5764-5766	NN	denotes	d.
T18683	5762-5763	CD	denotes	6
T18682	5758-5761	IN	denotes	for
T18681	5756-5757	NNP	denotes	C
T18680	5755-5756	CD	denotes	°
T18679	5752-5754	CD	denotes	80
T18678	5751-5752	CD	denotes	−
T18677	5748-5750	IN	denotes	at
T18676	5746-5747	-RRB-	denotes	)
T18675	5740-5746	NNPS	denotes	States
T18674	5733-5739	NNP	denotes	United
T18673	5731-5732	,	denotes	,
T18672	5727-5731	NNP	denotes	York
T18671	5723-5726	NNP	denotes	New
T19057	6529-6533	CD	denotes	1000
T19056	6525-6528	CC	denotes	and
T19055	6523-6524	,	denotes	,
T19054	6521-6523	NN	denotes	ng
T19053	6517-6520	CD	denotes	500
T19052	6515-6516	,	denotes	,
T18670	5721-5722	,	denotes	,
T18669	5712-5721	NNP	denotes	Rochester
T18668	5710-5711	,	denotes	,
T18667	5705-5710	NNP	denotes	Kodak
T18666	5704-5705	-LRB-	denotes	(
T18665	5699-5703	NN	denotes	film
T18664	5693-5698	NN	denotes	X-ray
T18663	5690-5692	TO	denotes	to
T18662	5681-5689	NN	denotes	exposure
T18661	5678-5680	TO	denotes	to
T18660	5672-5677	RB	denotes	prior
T18659	5670-5671	NNP	denotes	C
T18658	5669-5670	CD	denotes	°
T18657	5666-5668	CD	denotes	60
T18656	5663-5665	IN	denotes	at
T18655	5659-5662	NNP	denotes	SDS
T18654	5657-5658	NN	denotes	%
T18653	5656-5657	CD	denotes	1
T18652	5654-5655	,	denotes	,
T18651	5651-5654	NNP	denotes	SSC
T18650	5649-5650	CD	denotes	×
T18649	5646-5649	CD	denotes	0.2
T18648	5641-5645	IN	denotes	with
T18647	5634-5640	VBN	denotes	washed
T18646	5629-5633	VBD	denotes	were
T18645	5623-5628	NNS	denotes	Blots
T18644	5621-5622	.	denotes	.
T18643	5613-5621	NN	denotes	solution
T18642	5599-5612	NN	denotes	hybridization
T18641	5595-5598	NNP	denotes	SDS
T18640	5593-5594	NN	denotes	%
T18639	5592-5593	CD	denotes	7
T18638	5583-5591	VBD	denotes	buffered
T18637	5573-5582	NN	denotes	phosphate
T18636	5570-5572	IN	denotes	in
T18635	5560-5569	VBN	denotes	performed
T18634	5556-5559	VBD	denotes	was
T18633	5542-5555	NN	denotes	hybridization
T18632	5538-5541	DT	denotes	The
T18631	5536-5537	.	denotes	.
T18630	5535-5536	-RRB-	denotes	)
T18629	5528-5535	NNP	denotes	Kingdom
T18628	5521-5527	NNP	denotes	United
T18627	5519-5520	,	denotes	,
T18615	5436-5442	JJ	denotes	random
T18614	5433-5435	IN	denotes	by
T18613	5431-5432	,	denotes	,
T18612	5427-5431	NNP	denotes	dCTP
T18611	5426-5427	NNP	denotes	]
T18610	5421-5426	NNP	denotes	α-32P
T18609	5420-5421	NNP	denotes	[
T18608	5415-5419	IN	denotes	with
T18607	5407-5414	VBN	denotes	labeled
T18606	5403-5406	CC	denotes	and
T18605	5401-5402	-RRB-	denotes	)
T18604	5397-5401	CD	denotes	1927
T18603	5392-5396	CD	denotes	1353
T18602	5380-5391	NNS	denotes	nucleotides
T18601	5379-5380	-LRB-	denotes	(
T18600	5372-5378	NNP	denotes	RT-PCR
T18599	5369-5371	IN	denotes	by
T18598	5359-5368	VBN	denotes	generated
T18597	5353-5358	NN	denotes	probe
T18596	5348-5352	JJ	denotes	Sox6
T18595	5346-5347	DT	denotes	a
T18594	5341-5345	IN	denotes	with
T18593	5330-5340	VBN	denotes	hybridized
T18592	5326-5329	VBD	denotes	was
T18591	5324-5325	-RRB-	denotes	)
T18590	5318-5324	NNPS	denotes	States
T18589	5311-5317	NNP	denotes	United
T18588	5309-5310	,	denotes	,
T18587	5301-5309	NNP	denotes	Maryland
T18586	5299-5300	,	denotes	,
T18585	5290-5299	NNP	denotes	Rockville
T18584	5288-5289	,	denotes	,
T18583	5281-5288	NNP	denotes	Seegene
T18582	5280-5281	-LRB-	denotes	(
T18581	5273-5279	NN	denotes	filter
T18580	5268-5272	NN	denotes	blot
T18579	5259-5267	NNP	denotes	Northern
T18578	5252-5258	NN	denotes	tissue
T18577	5242-5251	JJ	denotes	embryonic
T18576	5236-5241	NN	denotes	mouse
T18575	5234-5235	DT	denotes	A
T18574	5232-5233	.	denotes	.
T18573	5228-5232	NN	denotes	blot
T18572	5219-5227	NNP	denotes	Northern
T18406	5083-5084	NN	denotes	×
T18405	5081-5083	CD	denotes	40
T18404	5080-5081	-LRB-	denotes	(
T18403	5074-5079	NNP	denotes	Nikon
T18402	5070-5073	CD	denotes	.17
T18401	5065-5070	CD	denotes	160/0
T18400	5061-5064	CD	denotes	.65
T18399	5057-5061	CD	denotes	40/0
T18398	5052-5056	NNP	denotes	Plan
T18397	5050-5051	NNP	denotes	E
T18396	5045-5049	VBD	denotes	were
T18395	5040-5044	VBN	denotes	used
T18394	5029-5039	NNS	denotes	Objectives
T18393	5027-5028	.	denotes	.
T18392	5017-5027	NN	denotes	microscope
T18391	5006-5016	NNP	denotes	Labophot-2
T18390	5000-5005	NNP	denotes	Nikon
T18389	4995-4999	IN	denotes	with
T18388	4986-4994	VBN	denotes	obtained
T18387	4981-4985	VBD	denotes	were
T18386	4974-4980	NNS	denotes	Images
T18385	4972-4973	.	denotes	.
T18384	4966-4972	NN	denotes	manner
T18383	4958-4965	JJ	denotes	similar
T18382	4956-4957	DT	denotes	a
T18381	4953-4955	IN	denotes	in
T18380	4944-4952	VBN	denotes	prepared
T18379	4939-4943	VBD	denotes	were
T18378	4937-4938	-RRB-	denotes	)
T18377	4934-4937	NN	denotes	dpc
T18376	4929-4933	CD	denotes	18.5
T18375	4925-4928	CC	denotes	and
T18374	4921-4924	NN	denotes	dpc
T18373	4916-4920	CD	denotes	14.5
T18372	4913-4915	IN	denotes	at
T18371	4912-4913	-LRB-	denotes	(
T18370	4904-4911	NNS	denotes	samples
T18369	4898-4903	NNP	denotes	Liver
T18368	4896-4897	.	denotes	.
T18367	4891-4896	NN	denotes	eosin
T18366	4887-4890	CC	denotes	and
T18365	4875-4886	NN	denotes	hematoxylin
T18364	4870-4874	IN	denotes	with
T18363	4862-4869	VBN	denotes	stained
T18362	4858-4861	CC	denotes	and
T18361	4856-4857	,	denotes	,
T18360	4854-4856	NN	denotes	μm
T18359	4852-4853	CD	denotes	5
T18358	4849-4851	IN	denotes	at
T18357	4839-4848	VBN	denotes	sectioned
T18356	4837-4838	,	denotes	,
T18355	4820-4837	JJ	denotes	paraffin-embedded
T18354	4818-4819	,	denotes	,
T18353	4810-4818	NN	denotes	formalin
T18352	4808-4809	NN	denotes	%
T18351	4806-4808	CD	denotes	10
T18350	4803-4805	IN	denotes	in
T18349	4797-4802	VBN	denotes	fixed
T18348	4792-4796	VBD	denotes	were
T18347	4787-4791	NNS	denotes	mice
T18346	4782-4786	CD	denotes	10.5
T18345	4778-4781	NN	denotes	day
T18344	4768-4777	JJ	denotes	postnatal
T18343	4764-4767	CC	denotes	and
T18342	4762-4763	,	denotes	,
T18341	4755-4762	NNS	denotes	embryos
T18340	4748-4754	JJ	denotes	mutant
T18339	4744-4747	CC	denotes	and
T18338	4741-4743	NNP	denotes	WT
T18337	4732-4740	JJ	denotes	14.5-dpc
T18336	4730-4731	,	denotes	,
T18335	4722-4730	NN	denotes	analysis
T18334	4716-4721	VBP	denotes	mount
T18333	4710-4715	JJ	denotes	whole
T18332	4706-4709	IN	denotes	For
T18331	4704-4705	.	denotes	.
T18330	4701-4704	NNP	denotes	DAF
T18329	4698-4700	IN	denotes	by
T18328	4693-4697	VBN	denotes	read
T18327	4689-4692	CC	denotes	and
T18326	4674-4688	JJ	denotes	Wright-stained
T18325	4669-4673	VBD	denotes	were
T18324	4662-4668	NNS	denotes	slides
T18323	4658-4661	DT	denotes	The
T18322	4656-4657	.	denotes	.
T18321	4652-4656	NNS	denotes	mice
T18320	4649-4651	NNP	denotes	WT
T18319	4645-4648	CC	denotes	and
T18318	4638-4644	JJ	denotes	mutant
T18317	4633-4637	DT	denotes	both
T18316	4628-4632	IN	denotes	from
T18315	4619-4627	VBN	denotes	prepared
T18314	4614-4618	VBD	denotes	were
T18313	4607-4613	NNS	denotes	smears
T18312	4601-4606	NN	denotes	blood
T18311	4590-4600	JJ	denotes	peripheral
T18310	4586-4589	CC	denotes	and
T18309	4572-4585	VBN	denotes	exsanguinated
T18308	4567-4571	VBD	denotes	were
T18307	4559-4566	NNS	denotes	embryos
T18306	4550-4558	JJ	denotes	18.5-dpc
T18305	4548-4549	.	denotes	.
T18304	4539-4548	NNP	denotes	Histology
T18044	3989-3992	NNP	denotes	RNA
T18043	3977-3988	VBN	denotes	transcribed
T18042	3971-3976	NN	denotes	vitro
T18041	3968-3970	IN	denotes	in
T18040	3962-3967	VBG	denotes	using
T18039	3960-3961	NNP	denotes	]
T18038	3958-3960	CD	denotes	52
T18037	3957-3958	NNP	denotes	[
T18036	3947-3956	VBN	denotes	described
T18035	3944-3946	IN	denotes	as
T18034	3930-3943	NN	denotes	hybridization
T18033	3925-3929	NN	denotes	situ
T18032	3922-3924	IN	denotes	in
T18031	3918-3921	IN	denotes	for
T18030	3908-3917	VBN	denotes	processed
T18029	3903-3907	VBD	denotes	were
T18028	3896-3902	NNS	denotes	Slides
T18027	3894-3895	.	denotes	.
T18026	3893-3894	-RRB-	denotes	)
T18025	3887-3893	NNPS	denotes	States
T18024	3880-3886	NNP	denotes	United
T18023	3878-3879	,	denotes	,
T18022	3866-3878	NNP	denotes	Pennsylvania
T18021	3864-3865	,	denotes	,
T18020	3857-3864	NNP	denotes	Chester
T18019	3852-3856	NNP	denotes	West
T18018	3850-3851	,	denotes	,
T18017	3847-3850	NNP	denotes	VWR
T18016	3846-3847	-LRB-	denotes	(
T18015	3839-3845	NNS	denotes	slides
T18014	3830-3838	VBN	denotes	modified
T18013	3823-3829	VB	denotes	charge
T18012	3820-3822	TO	denotes	to
T18011	3812-3819	VBD	denotes	adhered
T18010	3808-3811	CC	denotes	and
T18436	5216-5217	.	denotes	.
T18435	5207-5216	JJ	denotes	available
T18434	5203-5206	VBP	denotes	are
T18433	5196-5202	NNS	denotes	images
T18432	5187-5195	NNP	denotes	Original
T18431	5185-5186	.	denotes	.
T18430	5181-5185	CD	denotes	4300
T18429	5173-5180	NNP	denotes	Coolpix
T18428	5167-5172	NNP	denotes	Nikon
T18427	5165-5166	DT	denotes	a
T18426	5161-5164	VBD	denotes	was
T18425	5154-5160	NN	denotes	camera
T18424	5150-5153	DT	denotes	The
T18423	5148-5149	.	denotes	.
T18422	5147-5148	-RRB-	denotes	)
T18421	5138-5147	NN	denotes	objective
T18420	5136-5137	CD	denotes	×
T18419	5133-5136	CD	denotes	100
T18418	5132-5133	-LRB-	denotes	(
T18417	5126-5131	NNP	denotes	Nikon
T18416	5122-5125	CD	denotes	.17
T18415	5117-5122	CD	denotes	160/0
T18414	5113-5116	NN	denotes	oil
T18413	5109-5112	CD	denotes	.25
T18412	5104-5109	CD	denotes	100/1
T18411	5099-5103	NNP	denotes	Plan
T18410	5097-5098	NNP	denotes	E
T18409	5095-5096	,	denotes	,
T18408	5094-5095	-RRB-	denotes	)
T18407	5085-5094	NN	denotes	objective
T17681	3532-3533	.	denotes	.
T17680	3531-3532	-RRB-	denotes	)
T17679	3525-3531	NNPS	denotes	States
T17678	3518-3524	NNP	denotes	United
T17677	3516-3517	,	denotes	,
T17676	3506-3516	NNP	denotes	Washington
T17675	3504-3505	,	denotes	,
T17674	3497-3504	NNP	denotes	Redmond
T17673	3496-3497	-LRB-	denotes	(
T17672	3490-3495	NNP	denotes	Excel
T17671	3480-3489	NNP	denotes	Microsoft
T17670	3477-3479	IN	denotes	in
T17669	3471-3476	NNP	denotes	GAPDH
T17668	3468-3470	TO	denotes	to
T17667	3457-3467	VBN	denotes	normalized
T17666	3453-3456	CC	denotes	and
T17665	3451-3452	-RRB-	denotes	)
T17664	3441-3451	NNPS	denotes	Biosystems
T17663	3433-3440	NNP	denotes	Applied
T17662	3432-3433	-LRB-	denotes	(
T17661	3423-3431	NNP	denotes	Software
T17660	3419-3422	NNP	denotes	SDS
T17659	3414-3418	CD	denotes	7000
T17658	3408-3413	NNP	denotes	Prism
T17657	3404-3407	NNP	denotes	ABI
T17656	3400-3403	DT	denotes	the
T17655	3397-3399	IN	denotes	in
T17654	3386-3396	VBN	denotes	calculated
T17653	3381-3385	VBD	denotes	were
T17652	3374-3380	NNS	denotes	values
T17651	3361-3373	JJ	denotes	quantitative
T17650	3352-3360	JJ	denotes	Relative
T17649	3350-3351	.	denotes	.
T17648	3342-3350	NN	denotes	reaction
T17647	3338-3341	NNP	denotes	PCR
T17646	3333-3337	DT	denotes	each
T17645	3329-3332	IN	denotes	for
T17644	3324-3328	VBN	denotes	done
T17643	3319-3323	VBD	denotes	were
T17642	3307-3318	NNS	denotes	Triplicates
T17641	3305-3306	.	denotes	.
T17640	3291-3305	NNS	denotes	amplifications
T17639	3287-3290	DT	denotes	the
T17638	3284-3286	IN	denotes	of
T17637	3273-3283	NN	denotes	efficiency
T17636	3269-3272	DT	denotes	the
T17635	3264-3268	VB	denotes	test
T17634	3261-3263	TO	denotes	to
T17633	3251-3260	VBN	denotes	performed
T17632	3246-3250	VBD	denotes	were
T17631	3237-3245	NNS	denotes	analyses
T17630	3231-3236	NN	denotes	curve
T17629	3222-3230	NNP	denotes	Standard
T17628	3220-3221	.	denotes	.
T17627	3217-3220	NNP	denotes	RNA
T17626	3211-3216	NN	denotes	input
T17625	3207-3210	IN	denotes	for
T17624	3199-3206	NN	denotes	control
T17623	3196-3198	IN	denotes	as
T17622	3191-3195	VBN	denotes	used
T17621	3186-3190	VBD	denotes	were
T17620	3179-3185	NNS	denotes	levels
T17619	3174-3178	NNP	denotes	mRNA
T17618	3168-3173	NNP	denotes	GAPDH
T17617	3166-3167	.	denotes	.
T17616	3158-3166	NN	denotes	supermix
T17615	3152-3157	JJ	denotes	green
T17614	3147-3151	NNP	denotes	SYBR
T17613	3144-3146	NN	denotes	μl
T17612	3139-3143	CD	denotes	12.5
T17611	3134-3138	IN	denotes	with
T17610	3125-3133	NN	denotes	reaction
T17609	3119-3124	JJ	denotes	25-μl
T17608	3117-3118	DT	denotes	a
T17607	3114-3116	IN	denotes	in
T17606	3104-3113	VBN	denotes	performed
T17605	3100-3103	VBD	denotes	was
T17604	3096-3099	NNP	denotes	PCR
T17603	3092-3095	DT	denotes	All
T17602	3090-3091	.	denotes	.
T17601	3082-3090	NN	denotes	facility
T17600	3077-3081	NN	denotes	core
T17599	3069-3076	NNP	denotes	Arizona
T17598	3066-3068	IN	denotes	of
T17597	3055-3065	NNP	denotes	University
T17596	3051-3054	DT	denotes	the
T17595	3048-3050	IN	denotes	at
T17594	3046-3047	-RRB-	denotes	)
T17593	3040-3046	NNPS	denotes	States
T17592	3033-3039	NNP	denotes	United
T17591	3031-3032	,	denotes	,
T17590	3021-3031	NNP	denotes	California
T17589	3019-3020	,	denotes	,
T17588	3015-3019	NNP	denotes	City
T17587	3008-3014	NNP	denotes	Foster
T18152	4536-4537	.	denotes	.
T18151	4527-4536	JJ	denotes	available
T18150	4523-4526	VBP	denotes	are
T18149	4516-4522	NNS	denotes	images
T18148	4507-4515	NNP	denotes	Original
T18147	4505-4506	.	denotes	.
T18146	4498-4505	NNS	denotes	plugins
T18145	4496-4497	-RRB-	denotes	)
T18144	4490-4496	NNPS	denotes	States
T18143	4483-4489	NNP	denotes	United
T18142	4481-4482	,	denotes	,
T18141	4473-4481	NNP	denotes	Carolina
T18140	4467-4472	NNP	denotes	North
T18139	4465-4466	,	denotes	,
T18138	4456-4465	NNP	denotes	Asheville
T18137	4454-4455	,	denotes	,
T18136	4446-4454	NNP	denotes	Graphics
T18135	4437-4445	NNP	denotes	Reindeer
T18134	4436-4437	-LRB-	denotes	(
T18133	4432-4435	FW	denotes	Pro
T18132	4426-4431	NNP	denotes	Fovea
T18131	4421-4425	IN	denotes	with
T18130	4412-4420	NN	denotes	software
T18129	4410-4411	-RRB-	denotes	)
T18128	4404-4410	NNPS	denotes	States
T18127	4397-4403	NNP	denotes	United
T18126	4395-4396	,	denotes	,
T18125	4385-4395	NNP	denotes	California
T18124	4383-4384	,	denotes	,
T18123	4379-4383	NNP	denotes	Jose
T18122	4375-4378	NNP	denotes	San
T18121	4373-4374	,	denotes	,
T18120	4368-4373	NNP	denotes	Adobe
T18119	4367-4368	-LRB-	denotes	(
T18118	4357-4366	NNP	denotes	Photoshop
T18117	4351-4356	VBG	denotes	using
T18107	4297-4298	-RRB-	denotes	)
T18106	4294-4297	CD	denotes	0.5
T18105	4292-4293	SYM	denotes	=
T18104	4289-4291	NNP	denotes	NA
T18103	4288-4289	-LRB-	denotes	(
T18102	4286-4287	NN	denotes	×
T18101	4284-4286	CD	denotes	10
T18100	4280-4283	CC	denotes	and
T18099	4278-4279	-RRB-	denotes	)
T18098	4274-4278	CD	denotes	0.04
T18097	4272-4273	SYM	denotes	=
T18096	4269-4271	NNP	denotes	NA
T18095	4268-4269	-LRB-	denotes	(
T18094	4266-4267	NN	denotes	×
T18093	4265-4266	CD	denotes	1
T18092	4260-4264	VBD	denotes	were
T18091	4255-4259	VBN	denotes	used
T18090	4244-4254	NNS	denotes	Objectives
T18089	4242-4243	.	denotes	.
T18088	4241-4242	-RRB-	denotes	)
T18087	4235-4241	NNPS	denotes	States
T18086	4228-4234	NNP	denotes	United
T18085	4226-4227	,	denotes	,
T18084	4218-4226	NNP	denotes	Michigan
T18083	4216-4217	,	denotes	,
T18082	4209-4216	NNP	denotes	Heights
T18081	4200-4208	NNP	denotes	Sterling
T18079	4187-4198	NNP	denotes	Instruments
T18078	4176-4186	NNP	denotes	Diagnostic
T18077	4175-4176	-LRB-	denotes	(
T18076	4168-4174	NN	denotes	camera
T18075	4160-4167	JJ	denotes	digital
T18074	4150-4159	JJ	denotes	RT-Slider
T18073	4145-4149	NNP	denotes	SPOT
T18072	4141-4144	CC	denotes	and
T18071	4139-4140	-RRB-	denotes	)
T18070	4133-4139	NNPS	denotes	States
T18069	4126-4132	NNP	denotes	United
T18068	4124-4125	,	denotes	,
T18067	4120-4124	NNP	denotes	York
T18066	4116-4119	NNP	denotes	New
T18065	4114-4115	,	denotes	,
T18064	4106-4114	NNP	denotes	Melville
T18063	4104-4105	,	denotes	,
T18062	4099-4104	NNP	denotes	Nikon
T18061	4098-4099	-LRB-	denotes	(
T18060	4087-4097	NN	denotes	microscope
T18059	4078-4086	NNP	denotes	Optiphot
T18058	4072-4077	NNP	denotes	Nikon
T18057	4070-4071	DT	denotes	a
T18056	4065-4069	IN	denotes	with
T18055	4056-4064	VBN	denotes	obtained
T18054	4051-4055	VBD	denotes	were
T18053	4044-4050	NNS	denotes	images
T18052	4032-4043	JJ	denotes	brightfield
T18051	4028-4031	CC	denotes	and
T18050	4018-4027	NNP	denotes	Darkfield
T18049	4016-4017	.	denotes	.
T18048	4013-4016	NNP	denotes	33P
T18047	4008-4012	IN	denotes	with
T18046	4000-4007	VBN	denotes	labeled
T18045	3993-3999	NNS	denotes	probes
T17586	3006-3007	,	denotes	,
T17585	2996-3006	NNPS	denotes	Biosystems
T17584	2988-2995	NNP	denotes	Applied
T17583	2987-2988	-LRB-	denotes	(
T17582	2979-2986	NNP	denotes	ABI7000
T17581	2976-2978	DT	denotes	an
T17580	2973-2975	IN	denotes	on
T17579	2969-2972	VBN	denotes	run
T17578	2965-2968	VBD	denotes	was
T17577	2951-2964	NN	denotes	amplification
T17576	2947-2950	NNP	denotes	PCR
T17575	2945-2946	,	denotes	,
T17574	2944-2945	-RRB-	denotes	)
T17573	2938-2944	NNPS	denotes	States
T17572	2931-2937	NNP	denotes	United
T17571	2929-2930	,	denotes	,
T17570	2919-2929	NNP	denotes	California
T17569	2917-2918	,	denotes	,
T17568	2909-2917	NNP	denotes	Hercules
T17567	2907-2908	,	denotes	,
T17566	2900-2907	NNP	denotes	Bio-Rad
T17565	2899-2900	-LRB-	denotes	(
T17564	2895-2898	NNP	denotes	ROX
T17563	2890-2894	IN	denotes	with
T17562	2886-2889	NN	denotes	kit
T17561	2877-2885	NN	denotes	supermix
T17560	2871-2876	JJ	denotes	green
T17559	2866-2870	NNP	denotes	SYBR
T17558	2862-2865	DT	denotes	the
T17557	2856-2861	VBG	denotes	Using
T17556	2854-2855	.	denotes	.
T17555	2853-2854	NN	denotes	′
T17554	2832-2853	NNP	denotes	ACGATCATATTGCCCAGGAG3
T17553	2831-2832	CD	denotes	′
T17552	2830-2831	CD	denotes	5
T17551	2828-2829	,	denotes	,
T17550	2821-2828	NNP	denotes	MHB1675
T17549	2817-2820	CC	denotes	and
T17548	2815-2816	:	denotes	;
T17547	2814-2815	NN	denotes	′
T17546	2793-2814	NNP	denotes	ATGGCCTGAATCACTTGGAC3
T17545	2792-2793	CD	denotes	′
T17544	2791-2792	CD	denotes	5
T17543	2789-2790	:	denotes	:
T17542	2782-2789	NNP	denotes	MHB1674
T17541	2780-2781	:	denotes	:
T17540	2774-2780	NN	denotes	globin
T17539	2770-2773	NN	denotes	min
T17538	2769-2770	NN	denotes	/
T17537	2765-2769	JJ	denotes	βmaj
T17536	2761-2764	IN	denotes	For
T17535	2759-2760	.	denotes	.
T17534	2758-2759	NN	denotes	′
T17533	2737-2758	NNP	denotes	ACCTCTGGGGTGAATTCCTT3
T17532	2736-2737	CD	denotes	′
T17531	2735-2736	CD	denotes	5
T17530	2733-2734	,	denotes	,
T17529	2726-2733	NNP	denotes	MHB1673
T17528	2722-2725	CC	denotes	and
T17527	2720-2721	:	denotes	;
T17526	2719-2720	NN	denotes	′
T17525	2698-2719	CD	denotes	TGGACAACCTCAAGGAGACC3
T17524	2697-2698	CD	denotes	′
T17523	2696-2697	CD	denotes	5
T17522	2694-2695	,	denotes	,
T17521	2687-2694	NNP	denotes	MHB1672
T17520	2685-2686	:	denotes	:
T17519	2679-2685	NN	denotes	globin
T17518	2675-2678	JJ	denotes	βH1
T17517	2671-2674	IN	denotes	For
T17516	2669-2670	.	denotes	.
T17515	2668-2669	NN	denotes	′
T17514	2647-2668	CD	denotes	CTTAACCGCATCCCCTACGG3
T17513	2646-2647	NN	denotes	′
T17512	2645-2646	CD	denotes	5
T17511	2643-2644	,	denotes	,
T17510	2636-2643	NNP	denotes	MHB1669
T17509	2632-2635	CC	denotes	and
T17508	2630-2631	:	denotes	;
T17507	2629-2630	NN	denotes	′
T17506	2607-2629	CD	denotes	CTACCCCCAGACGAAGACCTA3
T17505	2606-2607	NN	denotes	′
T17504	2605-2606	CD	denotes	5
T17503	2603-2604	,	denotes	,
T17502	2596-2603	CD	denotes	MHB1668
T17501	2594-2595	:	denotes	:
T17500	2588-2594	NN	denotes	globin
T17499	2586-2587	JJ	denotes	ζ
T17498	2582-2585	IN	denotes	For
T17497	2580-2581	.	denotes	.
T17496	2579-2580	NN	denotes	′
T17495	2557-2579	NNP	denotes	GAAGCAGAGGACAAGTTCCCA3
T17494	2556-2557	CD	denotes	′
T17493	2555-2556	CD	denotes	5
T17492	2553-2554	,	denotes	,
T17491	2546-2553	NNP	denotes	MHB1667
T17490	2542-2545	CC	denotes	and
T17489	2540-2541	:	denotes	;
T17488	2539-2540	NN	denotes	′
T17487	2517-2539	CD	denotes	TGGCCTGTGGAGTAAGGTCAA3
T17486	2516-2517	CD	denotes	′
T16996	2253-2254	.	denotes	.
T16995	2252-2253	-RRB-	denotes	)
T16994	2250-2252	CD	denotes	18
T16993	2249-2250	CD	denotes	−
T16992	2246-2248	TO	denotes	to
T16991	2243-2245	CD	denotes	63
T16990	2242-2243	FW	denotes	−
T16989	2241-2242	-LRB-	denotes	(
T16988	2239-2240	NN	denotes	′
T16987	2238-2239	CD	denotes	3
T16986	2191-2237	NNP	denotes	CCGCTCGAGAATGCATGCCAAGGGTCAGATGAGTGTCTGCGAAGAA
T16985	2190-2191	NN	denotes	′
T17485	2515-2516	CD	denotes	5
T17484	2513-2514	,	denotes	,
T17483	2506-2513	NNP	denotes	MHB1666
T17482	2504-2505	:	denotes	:
T17481	2498-2504	NN	denotes	globin
T17480	2495-2497	JJ	denotes	ɛy
T17479	2491-2494	IN	denotes	For
T17478	2489-2490	.	denotes	.
T17477	2478-2489	NN	denotes	specificity
T17476	2470-2477	VB	denotes	confirm
T17475	2467-2469	TO	denotes	to
T17474	2458-2466	NN	denotes	database
T17473	2453-2457	NNP	denotes	NCBI
T17472	2449-2452	DT	denotes	the
T17471	2441-2448	IN	denotes	against
T17470	2432-2440	VBN	denotes	searched
T17469	2427-2431	VBD	denotes	were
T17468	2419-2426	NNS	denotes	primers
T17467	2415-2418	DT	denotes	All
T17466	2413-2414	.	denotes	.
T17465	2412-2413	NNP	denotes	]
T17464	2410-2412	CD	denotes	51
T17463	2409-2410	NNP	denotes	[
T17462	2398-2408	NNP	denotes	Primerbank
T17461	2393-2397	IN	denotes	from
T17460	2384-2392	VBN	denotes	obtained
T17459	2379-2383	VBD	denotes	were
T17458	2373-2378	NNS	denotes	genes
T17457	2366-2372	NN	denotes	globin
T17456	2363-2365	IN	denotes	of
T17455	2349-2362	NN	denotes	amplification
T17454	2345-2348	NNP	denotes	PCR
T17453	2340-2344	NNP	denotes	cDNA
T17452	2336-2339	IN	denotes	for
T17451	2328-2335	NNS	denotes	Primers
T17450	2326-2327	.	denotes	.
T17449	2322-2326	NNP	denotes	cDNA
T17448	2319-2321	TO	denotes	to
T17447	2307-2318	VBN	denotes	transcribed
T17446	2299-2306	JJ	denotes	reverse
T17445	2293-2298	JJ	denotes	first
T17444	2289-2292	VBD	denotes	was
T17443	2285-2288	NNP	denotes	RNA
T17442	2283-2284	.	denotes	.
T17441	2279-2283	NNP	denotes	mRNA
T17440	2272-2278	NN	denotes	globin
T17439	2269-2271	IN	denotes	of
T17438	2256-2268	NN	denotes	Quantitation
T16984	2189-2190	CD	denotes	5
T16983	2187-2188	,	denotes	,
T16982	2180-2187	NNP	denotes	MHB1663
T16981	2176-2179	CC	denotes	and
T16980	2174-2175	:	denotes	;
T16979	2173-2174	-RRB-	denotes	)
T16978	2171-2173	CD	denotes	16
T16977	2170-2171	CD	denotes	−
T16976	2167-2169	TO	denotes	to
T16975	2164-2166	CD	denotes	63
T16974	2163-2164	FW	denotes	−
T16973	2162-2163	-LRB-	denotes	(
T16972	2160-2161	NN	denotes	′
T16971	2112-2160	CD	denotes	CCGCTCGAGAATGCAGAACAAAGGGTCAGATGAGTGTCTGCGAAGAA3
T16970	2111-2112	NN	denotes	′
T16969	2110-2111	CD	denotes	5
T16968	2108-2109	,	denotes	,
T16967	2101-2108	NNP	denotes	MHB1662
T16966	2099-2100	:	denotes	;
T16965	2098-2099	-RRB-	denotes	)
T16964	2096-2098	CD	denotes	19
T16963	2095-2096	CD	denotes	−
T16962	2092-2094	TO	denotes	to
T16961	2089-2091	CD	denotes	63
T16960	2088-2089	FW	denotes	−
T16959	2087-2088	-LRB-	denotes	(
T16958	2085-2086	NN	denotes	′
T16957	2039-2085	CD	denotes	CCGCTCGAGAATGCAGTGCCAAGGGTCAGAACATTGTCTGCGAAG3
T16956	2038-2039	NN	denotes	′
T16955	2037-2038	CD	denotes	5
T16954	2035-2036	,	denotes	,
T16953	2028-2035	CD	denotes	MHB1661
T16952	2026-2027	:	denotes	:
T16951	2019-2026	VBP	denotes	include
T16950	2008-2018	NNS	denotes	constructs
T16949	1999-2007	NN	denotes	reporter
T16948	1990-1998	NN	denotes	promoter
T16947	1987-1989	NN	denotes	ɛy
T16946	1975-1986	VBN	denotes	mutagenized
T16945	1969-1974	DT	denotes	these
T16944	1960-1968	VB	denotes	generate
T16943	1957-1959	TO	denotes	to
T16942	1952-1956	VBN	denotes	used
T16941	1944-1951	NNS	denotes	primers
T16940	1936-1943	RB	denotes	Forward
T16939	1934-1935	.	denotes	.
T16938	1931-1934	NNP	denotes	PCR
T16937	1928-1930	IN	denotes	by
T16936	1923-1927	VBN	denotes	done
T16935	1918-1922	VBD	denotes	were
T16934	1909-1917	NN	denotes	promoter
T16933	1906-1908	JJ	denotes	ɛy
T16932	1902-1905	DT	denotes	the
T16931	1899-1901	IN	denotes	of
T16930	1893-1898	NNS	denotes	sites
T16929	1885-1892	JJ	denotes	binding
T16928	1875-1884	NN	denotes	consensus
T16927	1866-1874	NNP	denotes	Sox/Sox6
T16926	1863-1865	IN	denotes	of
T16925	1851-1862	NNP	denotes	Mutagenesis
T16924	1849-1850	.	denotes	.
T16923	1848-1849	NNP	denotes	]
T16922	1846-1848	CD	denotes	32
T16921	1845-1846	NNP	denotes	[
T16920	1838-1844	NNS	denotes	others
T16919	1835-1837	IN	denotes	by
T16918	1825-1834	VBN	denotes	described
T16917	1822-1824	IN	denotes	as
T16916	1820-1821	,	denotes	,
T16915	1811-1820	VBN	denotes	generated
T16914	1807-1810	VBD	denotes	was
T16913	1800-1806	NN	denotes	domain
T16912	1796-1799	NNP	denotes	HMG
T16911	1792-1795	DT	denotes	the
T16910	1786-1791	VBZ	denotes	lacks
T16909	1781-1785	WDT	denotes	that
T16908	1779-1780	-RRB-	denotes	)
T16907	1777-1779	CD	denotes	.1
T16906	1761-1777	JJ	denotes	Sox6-ΔHMG-pcDNA3
T16905	1760-1761	-LRB-	denotes	(
T16904	1750-1759	VBP	denotes	construct
T16903	1735-1749	NN	denotes	overexpression
T16902	1730-1734	JJ	denotes	Sox6
T16901	1726-1729	DT	denotes	the
T16900	1723-1725	IN	denotes	of
T16899	1715-1722	NN	denotes	version
T16898	1705-1714	VBN	denotes	truncated
T16897	1703-1704	DT	denotes	A
T16896	1701-1702	.	denotes	.
T16895	1697-1701	NNP	denotes	Sox6
T16894	1685-1696	VB	denotes	overexpress
T16893	1682-1684	TO	denotes	to
T16892	1677-1681	VBN	denotes	used
T16891	1673-1676	VBD	denotes	was
T16890	1671-1672	NNP	denotes	]
T16889	1669-1671	CD	denotes	15
T16888	1668-1669	NNP	denotes	[
T16887	1665-1667	CD	denotes	.1
T16886	1654-1665	JJ	denotes	Sox6-pcDNA3
T16885	1652-1653	.	denotes	.
T16884	1651-1652	-RRB-	denotes	)
T16873	1599-1600	:	denotes	:
T16872	1595-1599	NN	denotes	site
T16871	1587-1594	NNP	denotes	HindIII
T16870	1580-1586	NN	denotes	primer
T16869	1572-1579	NN	denotes	reverse
T16868	1568-1571	DT	denotes	the
T16867	1563-1567	IN	denotes	with
T16866	1551-1562	NN	denotes	combination
T16865	1548-1550	IN	denotes	in
T16864	1543-1547	VBN	denotes	used
T16863	1538-1542	VBD	denotes	were
T16862	1530-1537	NNS	denotes	primers
T16861	1522-1529	RB	denotes	forward
T16860	1518-1521	DT	denotes	All
T16859	1516-1517	.	denotes	.
T16858	1515-1516	-RRB-	denotes	)
T16857	1514-1515	CD	denotes	9
T16856	1513-1514	CD	denotes	−
T16855	1510-1512	TO	denotes	to
T16854	1507-1509	CD	denotes	37
T16853	1506-1507	FW	denotes	−
T16852	1505-1506	-LRB-	denotes	(
T16851	1503-1504	NN	denotes	′
T16850	1502-1503	CD	denotes	3
T16849	1472-1501	NNP	denotes	CCGCTCGAGGTCTGCGAAGAATAAAAGGC
T16848	1471-1472	NN	denotes	′
T16847	1470-1471	CD	denotes	5
T16846	1468-1469	,	denotes	,
T16845	1461-1468	NNP	denotes	MHB1507
T16844	1457-1460	CC	denotes	and
T16843	1455-1456	:	denotes	;
T16842	1454-1455	-RRB-	denotes	)
T16841	1452-1454	CD	denotes	34
T16840	1451-1452	CD	denotes	−
T16839	1448-1450	TO	denotes	to
T16838	1445-1447	CD	denotes	63
T16837	1444-1445	FW	denotes	−
T16836	1443-1444	-LRB-	denotes	(
T16835	1441-1442	NN	denotes	′
T16834	1410-1441	CD	denotes	CCGCTCGAGAATGCAGAACAAAGGGTCAGA3
T16833	1409-1410	NN	denotes	′
T16832	1408-1409	CD	denotes	5
T16831	1406-1407	,	denotes	,
T16830	1399-1406	NNP	denotes	MHB1532
T16829	1397-1398	:	denotes	;
T16828	1396-1397	-RRB-	denotes	)
T16827	1394-1396	CD	denotes	58
T16826	1393-1394	CD	denotes	−
T16825	1390-1392	TO	denotes	to
T16824	1387-1389	CD	denotes	85
T16823	1386-1387	FW	denotes	−
T16822	1385-1386	-LRB-	denotes	(
T16821	1383-1384	NN	denotes	′
T16820	1354-1383	CD	denotes	CCGCTCGAGCTGACCAATGGCTTCAAAG3
T16819	1353-1354	NN	denotes	′
T16818	1352-1353	CD	denotes	5
T16817	1350-1351	,	denotes	,
T16816	1343-1350	NNP	denotes	MHB1506
T16815	1341-1342	:	denotes	;
T16814	1340-1341	-RRB-	denotes	)
T16813	1337-1340	CD	denotes	169
T16812	1336-1337	CD	denotes	−
T16811	1333-1335	TO	denotes	to
T16810	1329-1332	CD	denotes	197
T16809	1328-1329	FW	denotes	−
T16808	1327-1328	-LRB-	denotes	(
T16807	1325-1326	NN	denotes	′
T16806	1296-1325	CD	denotes	CCGCTCGAGGGAGCCAAAAAAAGAATGC3
T16805	1295-1296	NN	denotes	′
T16804	1294-1295	CD	denotes	5
T16803	1292-1293	,	denotes	,
T16802	1285-1292	NNP	denotes	MHB1505
T16801	1283-1284	:	denotes	;
T16800	1282-1283	-RRB-	denotes	)
T16799	1279-1282	CD	denotes	605
T16798	1278-1279	CD	denotes	−
T16797	1275-1277	TO	denotes	to
T16796	1271-1274	CD	denotes	634
T16795	1270-1271	FW	denotes	−
T16794	1269-1270	-LRB-	denotes	(
T16793	1267-1268	NN	denotes	′
T16792	1236-1267	CD	denotes	CCGCTCGAGTCTCTACACTGTCACTCCCTG3
T16791	1235-1236	NN	denotes	′
T16790	1234-1235	CD	denotes	5
T16789	1232-1233	,	denotes	,
T16788	1225-1232	NNP	denotes	MHB1503
T16787	1223-1224	:	denotes	;
T16786	1222-1223	-RRB-	denotes	)
T16785	1218-1222	CD	denotes	2052
T16784	1217-1218	CD	denotes	−
T16783	1214-1216	TO	denotes	to
T16782	1209-1213	CD	denotes	2077
T16781	1208-1209	FW	denotes	−
T16780	1207-1208	-LRB-	denotes	(
T16779	1205-1206	NN	denotes	′
T16778	1178-1205	CD	denotes	CCGCTCGAGTGCTAGGCAAACACTCA3
T16777	1177-1178	NN	denotes	′
T16776	1176-1177	CD	denotes	5
T16775	1174-1175	,	denotes	,
T16774	1167-1174	NNP	denotes	MHB1457
T16773	1165-1166	:	denotes	:
T16772	1158-1165	VBP	denotes	include
T16771	1153-1157	NN	denotes	site
T16770	1148-1152	NNP	denotes	XhoI
T16769	1145-1147	DT	denotes	an
T16768	1140-1144	IN	denotes	with
T16767	1132-1139	NNS	denotes	primers
T16766	1124-1131	RB	denotes	Forward
T16765	1122-1123	.	denotes	.
T16764	1113-1122	RB	denotes	similarly
T16763	1103-1112	VBN	denotes	generated
T16762	1098-1102	VBD	denotes	were
T16761	1089-1097	NN	denotes	promoter
T16760	1086-1088	JJ	denotes	ɛy
T16759	1082-1085	DT	denotes	the
T16758	1079-1081	IN	denotes	of
T16757	1068-1078	NNS	denotes	constructs
T16756	1059-1067	NN	denotes	deletion
T16755	1056-1058	IN	denotes	of
T16754	1049-1055	NN	denotes	series
T16753	1047-1048	DT	denotes	A
T16752	1045-1046	.	denotes	.
T16751	1037-1045	NN	denotes	promoter
T16750	1034-1036	JJ	denotes	ɛy
T16749	1030-1033	DT	denotes	the
T16748	1027-1029	IN	denotes	by
T16747	1020-1026	VBN	denotes	driven
T16746	1017-1019	VBZ	denotes	is
T16745	1006-1016	NN	denotes	expression
T16744	995-1005	NN	denotes	luciferase
T16743	989-994	WDT	denotes	which
T16742	986-988	IN	denotes	in
T16741	976-985	VB	denotes	construct
T16740	967-975	NN	denotes	reporter
T16739	965-966	DT	denotes	a
T16738	962-964	IN	denotes	in
T16737	952-961	VBG	denotes	resulting
T16736	950-951	,	denotes	,
T16735	943-950	NN	denotes	plasmid
T16734	937-942	JJ	denotes	Basic
T16733	932-936	JJ	denotes	pGL3
T16732	926-931	JJ	denotes	above
T16731	922-925	DT	denotes	the
T16730	919-921	IN	denotes	in
T16729	910-918	NN	denotes	promoter
T16728	907-909	JJ	denotes	ɛy
T16727	903-906	DT	denotes	the
T16726	900-902	IN	denotes	of
T16725	891-899	RB	denotes	upstream
T16724	882-890	VBN	denotes	inserted
T16723	877-881	RB	denotes	then
T16722	873-876	VBD	denotes	was
T16721	871-872	-RRB-	denotes	)
T16720	870-871	NNP	denotes	]
T16719	868-870	CD	denotes	31
T16718	867-868	NNP	denotes	[
T16717	865-866	:	denotes	;
T16716	862-865	NNP	denotes	LCR
T16715	856-861	NN	denotes	micro
T16714	855-856	-LRB-	denotes	(
T16713	851-854	CD	denotes	9.3
T16712	845-850	NNP	denotes	μLCRβ
T16711	842-844	IN	denotes	of
T16710	833-841	NN	denotes	fragment
T16709	823-832	NNP	denotes	SstI/XhoI
T16708	816-822	JJ	denotes	2.5-kb
T16707	814-815	DT	denotes	A
T16706	812-813	.	denotes	.
T16705	811-812	-RRB-	denotes	)
T16704	805-811	NNPS	denotes	States
T16703	798-804	NNP	denotes	United
T16702	796-797	,	denotes	,
T16701	787-796	NNP	denotes	Wisconsin
T16700	785-786	,	denotes	,
T16699	778-785	NNP	denotes	Madison
T16698	776-777	,	denotes	,
T16697	769-776	NNP	denotes	Promega
T16696	768-769	-LRB-	denotes	(
T16695	762-767	NNP	denotes	Basic
T16694	757-761	NNP	denotes	pGL3
T16693	754-756	IN	denotes	in
T16692	749-753	NN	denotes	gene
T16691	738-748	NN	denotes	luciferase
T16690	730-737	JJ	denotes	firefly
T16689	726-729	DT	denotes	the
T16688	723-725	IN	denotes	of
T16687	714-722	RB	denotes	upstream
T16686	705-713	NN	denotes	fragment
T16685	696-704	NN	denotes	promoter
T16684	693-695	JJ	denotes	ɛy
T16683	689-692	DT	denotes	the
T16682	683-688	VB	denotes	clone
T16681	680-682	TO	denotes	to
T16680	675-679	VBN	denotes	used
T16679	670-674	VBD	denotes	were
T16678	665-669	WDT	denotes	that
T16677	659-664	NNS	denotes	sites
T16676	651-658	NNP	denotes	HindIII
T16675	647-650	CC	denotes	and
T16674	642-646	NNP	denotes	XhoI
T16673	632-641	VBD	denotes	contained
T16672	624-631	NNS	denotes	primers
T16671	620-623	NNP	denotes	PCR
T16670	616-619	DT	denotes	The
T16669	614-615	.	denotes	.
T16668	606-614	NN	denotes	database
T16667	604-605	-RRB-	denotes	)
T16666	599-604	NNP	denotes	Mouse
T16665	596-598	IN	denotes	of
T16664	585-595	NNP	denotes	Annotation
T16663	574-584	NNP	denotes	Functional
T16662	573-574	-LRB-	denotes	(
T16661	566-572	NNP	denotes	Fantom
T16660	562-565	DT	denotes	the
T16659	559-561	IN	denotes	in
T16658	554-558	NN	denotes	cDNA
T16635	427-428	NNP	denotes	[
T16634	422-426	NN	denotes	site
T16633	418-421	NN	denotes	cap
T16632	413-417	NN	denotes	gene
T16631	406-412	NN	denotes	globin
T16630	404-405	NN	denotes	ɛ
T16629	400-403	DT	denotes	the
T16628	397-399	IN	denotes	of
T16627	388-396	RB	denotes	upstream
T16626	379-387	NN	denotes	sequence
T16625	376-378	IN	denotes	of
T16624	373-375	NN	denotes	kb
T16623	371-372	CD	denotes	2
T16622	365-370	IN	denotes	about
T16621	354-364	VBG	denotes	containing
T16620	345-353	NN	denotes	fragment
T16619	339-344	NNP	denotes	EcoRI
T16618	332-338	JJ	denotes	3.7-kb
T16617	330-331	DT	denotes	a
T16616	323-329	IN	denotes	within
T16615	315-322	VBN	denotes	located
T16614	311-314	VBP	denotes	are
T16613	301-310	VBG	denotes	silencing
T16612	296-300	NN	denotes	gene
T16611	294-295	NN	denotes	ɛ
T16610	290-293	IN	denotes	for
T16609	281-289	VBN	denotes	required
T16608	271-280	NNS	denotes	sequences
T16607	267-270	DT	denotes	all
T16606	262-266	IN	denotes	that
T16605	256-261	VBN	denotes	shown
T16604	251-255	VBN	denotes	been
T16603	247-250	VBZ	denotes	has
T16602	244-246	PRP	denotes	it
T16601	236-243	IN	denotes	because
T16600	234-235	,	denotes	,
T16599	230-234	VBN	denotes	used
T16598	226-229	VBD	denotes	was
T16597	224-225	-RRB-	denotes	)
T16596	221-224	NNP	denotes	ATG
T16595	220-221	-LRB-	denotes	(
T16594	214-219	NN	denotes	codon
T16593	203-213	NN	denotes	initiation
T16592	196-202	NN	denotes	globin
T16591	193-195	JJ	denotes	ɛy
T16590	189-192	DT	denotes	the
T16589	186-188	IN	denotes	of
T16588	177-185	RB	denotes	upstream
T16587	168-176	NN	denotes	fragment
T16586	161-167	JJ	denotes	2.2-kb
T16585	159-160	DT	denotes	A
T16584	157-158	.	denotes	.
T16583	149-157	NN	denotes	promoter
T16582	140-148	JJ	denotes	proximal
T16581	137-139	JJ	denotes	ɛy
T16580	133-136	DT	denotes	the
T16579	130-132	IN	denotes	of
T16578	116-129	NN	denotes	amplification
T16577	112-115	NNP	denotes	PCR
T16576	109-111	IN	denotes	by
T16575	99-108	VBN	denotes	generated
T16574	95-98	VBD	denotes	was
T16573	93-94	-RRB-	denotes	)
T16572	88-93	JJ	denotes	E-luc
T16571	87-88	-LRB-	denotes	(
T16570	79-86	NN	denotes	plasmid
T16569	70-78	NN	denotes	reporter
T16568	61-69	NN	denotes	deletion
T16567	52-60	NN	denotes	promoter
T16566	49-51	JJ	denotes	ɛy
T16565	45-48	DT	denotes	The
T16564	43-44	.	denotes	.
T16563	31-43	NN	denotes	construction
T16562	23-30	JJ	denotes	Plasmid
T16883	1648-1651	CD	denotes	+20
T16882	1645-1647	TO	denotes	to
T16881	1641-1644	CD	denotes	+45
T16880	1640-1641	-LRB-	denotes	(
T16879	1638-1639	NN	denotes	′
T16878	1612-1638	CD	denotes	CGGAAGCTTGGGAGGTTGCTGGTGA3
T16877	1611-1612	NN	denotes	′
T16876	1610-1611	CD	denotes	5
T16875	1608-1609	,	denotes	,
T16874	1601-1608	NNP	denotes	MHB1477
T16657	546-553	JJS	denotes	longest
T16656	542-545	DT	denotes	the
T16655	539-541	IN	denotes	on
T16654	533-538	VBN	denotes	based
T16653	530-532	VBZ	denotes	is
T16652	525-529	NN	denotes	site
T16651	519-524	NN	denotes	start
T16650	503-518	JJ	denotes	transcriptional
T16649	499-502	DT	denotes	The
T16648	497-498	.	denotes	.
T16647	493-497	NN	denotes	site
T16646	487-492	VB	denotes	start
T16645	473-486	NN	denotes	transcription
T16644	469-472	DT	denotes	the
T16643	466-468	TO	denotes	to
T16642	457-465	JJ	denotes	relative
T16641	454-456	VBZ	denotes	is
T16640	444-453	NN	denotes	numbering
T16639	433-443	NNP	denotes	Nucleotide
T16638	431-432	.	denotes	.
T16637	430-431	NNP	denotes	]
T16636	428-430	CD	denotes	50
T18002	3776-3778	IN	denotes	in
T18001	3767-3775	VBN	denotes	embedded
T18009	3806-3807	,	denotes	,
T18008	3804-3806	NN	denotes	μm
T18007	3802-3803	CD	denotes	5
T18006	3799-3801	IN	denotes	at
T18005	3789-3798	VBN	denotes	sectioned
T18004	3787-3788	,	denotes	,
T18003	3779-3787	NN	denotes	paraffin
T18000	3765-3766	,	denotes	,
T17999	3749-3765	NN	denotes	paraformaldehyde
T17998	3747-3748	NN	denotes	%
T17997	3746-3747	CD	denotes	4
T17996	3743-3745	IN	denotes	in
T17995	3733-3742	NN	denotes	immersion
T17994	3730-3732	IN	denotes	by
T17993	3720-3729	RB	denotes	overnight
T17992	3714-3719	VBN	denotes	fixed
T17991	3709-3713	VBD	denotes	were
T17990	3701-3708	NNS	denotes	Embryos
T17989	3699-3700	.	denotes	.
T17988	3695-3699	CD	denotes	1927
T17987	3690-3694	CD	denotes	1353
T17986	3678-3689	NNS	denotes	nucleotides
T17985	3673-3677	JJ	denotes	Sox6
T17984	3667-3672	NN	denotes	mouse
T17983	3663-3666	CC	denotes	and
T17982	3661-3662	:	denotes	;
T17981	3658-3661	CD	denotes	549
T17980	3654-3657	CD	denotes	458
T17979	3642-3653	NNS	denotes	nucleotides
T17978	3635-3641	NN	denotes	globin
T17977	3630-3634	NN	denotes	βmaj
T17976	3628-3629	:	denotes	;
T17975	3625-3628	CD	denotes	584
T17974	3621-3624	CD	denotes	509
T17973	3609-3620	NNS	denotes	nucleotides
T17972	3602-3608	NN	denotes	globin
T17971	3599-3601	JJ	denotes	ɛy
T17970	3592-3598	VB	denotes	murine
T17969	3589-3591	TO	denotes	to
T17968	3580-3588	VBN	denotes	designed
T17967	3575-3579	VBD	denotes	were
T17966	3568-3574	NNS	denotes	probes
T17965	3558-3567	JJ	denotes	Antisense
T17964	3556-3557	.	denotes	.
T17963	3543-3556	NN	denotes	hybridization
T17962	3538-3542	NN	denotes	situ
T17961	3535-3537	IN	denotes	In
T18116	4342-4350	VBN	denotes	combined
T18115	4338-4341	CC	denotes	and
T18114	4336-4337	,	denotes	,
T18113	4323-4336	VBN	denotes	pseudocolored
T18112	4321-4322	,	denotes	,
T18111	4312-4321	VBN	denotes	processed
T18110	4307-4311	VBD	denotes	were
T18109	4300-4306	NNS	denotes	Images
T18108	4298-4299	.	denotes	.
T18626	5512-5519	NNP	denotes	England
T18625	5510-5511	,	denotes	,
T18624	5495-5510	NNP	denotes	Buckinghamshire
T18623	5493-5494	,	denotes	,
T18622	5482-5493	NNP	denotes	Biosciences
T18621	5473-5481	NNP	denotes	Amersham
T18620	5471-5472	:	denotes	;
T18619	5460-5471	NNP	denotes	RediprimeII
T18618	5459-5460	-LRB-	denotes	(
T18617	5450-5458	VBG	denotes	labeling
T18616	5443-5449	NN	denotes	primer
T19048	6501-6503	PRP	denotes	we
T19047	6499-6500	,	denotes	,
T19046	6493-6499	NN	denotes	effect
T19045	6486-6492	NN	denotes	dosage
T19044	6483-6485	IN	denotes	of
T19043	6476-6482	NNS	denotes	assays
T19042	6473-6475	IN	denotes	In
T19041	6471-6472	.	denotes	.
T19040	6470-6471	-RRB-	denotes	)
T19039	6468-6470	NN	denotes	ng
T19038	6463-6467	CD	denotes	1000
T19037	6462-6463	-LRB-	denotes	(
T19326	6899-6910	NN	denotes	translation
T19325	6895-6898	DT	denotes	The
T19324	6893-6894	.	denotes	.
T19323	6892-6893	NNP	denotes	]
T19322	6890-6892	CD	denotes	15
T19321	6889-6890	NNP	denotes	[
T19320	6882-6888	IN	denotes	before
T19319	6872-6881	VBN	denotes	described
T19318	6868-6871	VBD	denotes	was
T19317	6866-6867	,	denotes	,
T19316	6864-6866	NNP	denotes	HA
T19315	6860-6863	CC	denotes	and
R11478	T16562	T16563	amod	Plasmid,construction
R11479	T16563	T16563	ROOT	construction,construction
R11480	T16564	T16563	punct	.,construction
R11481	T16565	T16570	det	The,plasmid
R11482	T16566	T16567	amod	ɛy,promoter
R11483	T16567	T16570	compound	promoter,plasmid
R11484	T16568	T16570	compound	deletion,plasmid
R11485	T16569	T16570	compound	reporter,plasmid
R11486	T16570	T16575	nsubjpass	plasmid,generated
R11487	T16571	T16570	punct	(,plasmid
R11488	T16572	T16570	appos	E-luc,plasmid
R11489	T16573	T16570	punct	),plasmid
R11490	T16574	T16575	auxpass	was,generated
R11491	T16575	T16575	ROOT	generated,generated
R11492	T16576	T16575	agent	by,generated
R11493	T16577	T16578	compound	PCR,amplification
R11494	T16578	T16576	pobj	amplification,by
R11495	T16579	T16578	prep	of,amplification
R11496	T16580	T16583	det	the,promoter
R11497	T16581	T16583	amod	ɛy,promoter
R11498	T16582	T16583	amod	proximal,promoter
R11499	T16583	T16579	pobj	promoter,of
R11500	T16584	T16575	punct	.,generated
R11501	T16585	T16587	det	A,fragment
R11502	T16586	T16587	compound	2.2-kb,fragment
R11503	T16587	T16588	compound	fragment,upstream
R11504	T16588	T16599	nsubjpass	upstream,used
R11505	T16589	T16588	prep	of,upstream
R11506	T16590	T16593	det	the,initiation
R11507	T16591	T16593	amod	ɛy,initiation
R11508	T16592	T16593	compound	globin,initiation
R11509	T16593	T16594	compound	initiation,codon
R11510	T16594	T16589	pobj	codon,of
R11511	T16595	T16596	punct	(,ATG
R11512	T16596	T16594	appos	ATG,codon
R11513	T16597	T16594	punct	),codon
R11514	T16598	T16599	auxpass	was,used
R11515	T16599	T16599	ROOT	used,used
R11516	T16600	T16599	punct	",",used
R11517	T16601	T16605	mark	because,shown
R11518	T16602	T16605	nsubjpass	it,shown
R11519	T16603	T16605	aux	has,shown
R11520	T16604	T16605	auxpass	been,shown
R11521	T16605	T16599	advcl	shown,used
R11522	T16606	T16615	mark	that,located
R11523	T16607	T16608	det	all,sequences
R11524	T16608	T16615	nsubjpass	sequences,located
R11525	T16609	T16608	acl	required,sequences
R11526	T16610	T16609	prep	for,required
R11527	T16611	T16612	compound	ɛ,gene
R11528	T16612	T16610	pobj	gene,for
R11529	T16613	T16612	acl	silencing,gene
R11530	T16614	T16615	auxpass	are,located
R11531	T16615	T16605	ccomp	located,shown
R11532	T16616	T16615	prep	within,located
R11533	T16617	T16620	det	a,fragment
R11534	T16618	T16620	compound	3.7-kb,fragment
R11535	T16619	T16620	compound	EcoRI,fragment
R11536	T16620	T16616	pobj	fragment,within
R11537	T16621	T16620	acl	containing,fragment
R11538	T16622	T16624	quantmod	about,kb
R11539	T16623	T16624	nummod	2,kb
R11540	T16624	T16621	dobj	kb,containing
R11541	T16625	T16624	prep	of,kb
R11542	T16626	T16625	pobj	sequence,of
R11543	T16627	T16621	advmod	upstream,containing
R11544	T16628	T16627	prep	of,upstream
R11545	T16629	T16634	det	the,site
R11546	T16630	T16634	nmod	ɛ,site
R11547	T16631	T16634	compound	globin,site
R11548	T16632	T16633	compound	gene,cap
R11549	T16633	T16634	compound	cap,site
R11550	T16634	T16628	pobj	site,of
R11551	T16635	T16634	appos	[,site
R11552	T16636	T16637	nummod	50,]
R11553	T16637	T16634	appos	],site
R11554	T16638	T16599	punct	.,used
R11555	T16639	T16640	compound	Nucleotide,numbering
R11556	T16640	T16641	nsubj	numbering,is
R11557	T16641	T16641	ROOT	is,is
R11558	T16642	T16641	acomp	relative,is
R11559	T16643	T16642	prep	to,relative
R11560	T16644	T16645	det	the,transcription
R11561	T16645	T16646	nsubj	transcription,start
R11562	T16646	T16641	conj	start,is
R11563	T16647	T16646	dobj	site,start
R11564	T16648	T16641	punct	.,is
R11565	T16649	T16652	det	The,site
R11566	T16650	T16652	amod	transcriptional,site
R11567	T16651	T16652	compound	start,site
R11568	T16652	T16654	nsubjpass	site,based
R11569	T16653	T16654	auxpass	is,based
R11570	T16654	T16654	ROOT	based,based
R11571	T16655	T16654	prep	on,based
R11572	T16656	T16658	det	the,cDNA
R11573	T16657	T16658	amod	longest,cDNA
R11574	T16658	T16655	pobj	cDNA,on
R11575	T16659	T16658	prep	in,cDNA
R11576	T16660	T16661	det	the,Fantom
R11577	T16661	T16659	pobj	Fantom,in
R11578	T16662	T16661	punct	(,Fantom
R11579	T16663	T16664	compound	Functional,Annotation
R11580	T16664	T16661	appos	Annotation,Fantom
R11581	T16665	T16664	prep	of,Annotation
R11582	T16666	T16665	pobj	Mouse,of
R11583	T16667	T16661	punct	),Fantom
R11584	T16668	T16658	conj	database,cDNA
R11585	T16669	T16654	punct	.,based
R11586	T16670	T16672	det	The,primers
R11587	T16671	T16672	compound	PCR,primers
R11588	T16672	T16673	nsubj	primers,contained
R11589	T16673	T16673	ROOT	contained,contained
R11590	T16674	T16677	nmod	XhoI,sites
R11591	T16675	T16674	cc	and,XhoI
R11592	T16676	T16674	conj	HindIII,XhoI
R11593	T16677	T16673	dobj	sites,contained
R11594	T16678	T16680	nsubjpass	that,used
R11595	T16679	T16680	auxpass	were,used
R11596	T16680	T16677	relcl	used,sites
R11597	T16681	T16682	aux	to,clone
R11598	T16682	T16680	xcomp	clone,used
R11599	T16683	T16686	det	the,fragment
R11600	T16684	T16686	amod	ɛy,fragment
R11601	T16685	T16686	compound	promoter,fragment
R11602	T16686	T16682	dobj	fragment,clone
R11603	T16687	T16682	advmod	upstream,clone
R11604	T16688	T16687	prep	of,upstream
R11605	T16689	T16692	det	the,gene
R11606	T16690	T16692	amod	firefly,gene
R11607	T16691	T16692	compound	luciferase,gene
R11608	T16692	T16688	pobj	gene,of
R11609	T16693	T16692	prep	in,gene
R11610	T16694	T16695	compound	pGL3,Basic
R11611	T16695	T16693	pobj	Basic,in
R11612	T16696	T16699	punct	(,Madison
R11613	T16697	T16699	nmod	Promega,Madison
R11614	T16698	T16697	punct	",",Promega
R11615	T16699	T16692	appos	Madison,gene
R11616	T16700	T16699	punct	",",Madison
R11617	T16701	T16699	npadvmod	Wisconsin,Madison
R11618	T16702	T16701	punct	",",Wisconsin
R11619	T16703	T16704	compound	United,States
R11620	T16704	T16701	appos	States,Wisconsin
R11621	T16705	T16699	punct	),Madison
R11622	T16706	T16673	punct	.,contained
R11623	T16707	T16710	det	A,fragment
R11624	T16708	T16710	amod	2.5-kb,fragment
R11625	T16709	T16710	compound	SstI/XhoI,fragment
R11626	T16710	T16724	nsubjpass	fragment,inserted
R11627	T16711	T16710	prep	of,fragment
R11628	T16712	T16713	quantmod	μLCRβ,9.3
R11629	T16713	T16711	pobj	9.3,of
R11630	T16714	T16724	punct	(,inserted
R11631	T16715	T16716	compound	micro,LCR
R11632	T16716	T16724	nsubjpass	LCR,inserted
R11633	T16717	T16720	punct	;,]
R11634	T16718	T16720	compound	[,]
R11635	T16719	T16720	nummod	31,]
R11636	T16720	T16716	appos	],LCR
R11637	T16721	T16720	punct	),]
R11638	T16722	T16724	auxpass	was,inserted
R11639	T16723	T16724	advmod	then,inserted
R11640	T16724	T16724	ROOT	inserted,inserted
R11641	T16725	T16724	advmod	upstream,inserted
R11642	T16726	T16725	prep	of,upstream
R11643	T16727	T16729	det	the,promoter
R11644	T16728	T16729	amod	ɛy,promoter
R11645	T16729	T16726	pobj	promoter,of
R11646	T16730	T16729	prep	in,promoter
R11647	T16731	T16735	det	the,plasmid
R11648	T16732	T16735	amod	above,plasmid
R11649	T16733	T16735	amod	pGL3,plasmid
R11650	T16734	T16735	amod	Basic,plasmid
R11651	T16735	T16730	pobj	plasmid,in
R11652	T16736	T16724	punct	",",inserted
R11653	T16737	T16724	advcl	resulting,inserted
R11654	T16738	T16737	prep	in,resulting
R11655	T16739	T16740	det	a,reporter
R11656	T16740	T16738	pobj	reporter,in
R11657	T16741	T16724	punct	construct,inserted
R11658	T16742	T16747	prep	in,driven
R11659	T16743	T16742	pobj	which,in
R11660	T16744	T16745	amod	luciferase,expression
R11661	T16745	T16747	nsubjpass	expression,driven
R11662	T16746	T16747	auxpass	is,driven
R11663	T16747	T16724	advcl	driven,inserted
R11664	T16748	T16747	agent	by,driven
R11665	T16749	T16751	det	the,promoter
R11666	T16750	T16751	amod	ɛy,promoter
R11667	T16751	T16748	pobj	promoter,by
R11668	T16752	T16724	punct	.,inserted
R11669	T16753	T16754	det	A,series
R11670	T16754	T16763	nsubjpass	series,generated
R11671	T16755	T16754	prep	of,series
R11672	T16756	T16757	compound	deletion,constructs
R11673	T16757	T16755	pobj	constructs,of
R11674	T16758	T16757	prep	of,constructs
R11675	T16759	T16761	det	the,promoter
R11676	T16760	T16761	amod	ɛy,promoter
R11677	T16761	T16758	pobj	promoter,of
R11678	T16762	T16763	auxpass	were,generated
R11679	T16763	T16763	ROOT	generated,generated
R11680	T16764	T16763	advmod	similarly,generated
R11681	T16765	T16763	punct	.,generated
R11682	T16766	T16767	amod	Forward,primers
R11683	T16767	T16772	nsubj	primers,include
R11684	T16768	T16767	prep	with,primers
R11685	T16769	T16771	det	an,site
R11686	T16770	T16771	compound	XhoI,site
R11687	T16771	T16768	pobj	site,with
R11688	T16772	T16772	ROOT	include,include
R11689	T16773	T16772	punct	:,include
R11690	T16774	T16772	dobj	MHB1457,include
R11691	T16775	T16774	punct	",",MHB1457
R11692	T16776	T16779	nummod	5,′
R11693	T16777	T16778	quantmod	′,CCGCTCGAGTGCTAGGCAAACACTCA3
R11694	T16778	T16779	nummod	CCGCTCGAGTGCTAGGCAAACACTCA3,′
R11695	T16779	T16774	appos	′,MHB1457
R11696	T16780	T16779	punct	(,′
R11697	T16781	T16779	nmod	−,′
R11698	T16782	T16781	pobj	2077,−
R11699	T16783	T16779	prep	to,′
R11700	T16784	T16783	pobj	−,to
R11701	T16785	T16784	appos	2052,−
R11702	T16786	T16784	punct	),−
R11703	T16787	T16783	punct	;,to
R11704	T16788	T16783	pobj	MHB1503,to
R11705	T16789	T16788	punct	",",MHB1503
R11706	T16790	T16791	nummod	5,′
R11707	T16791	T16788	appos	′,MHB1503
R11708	T16792	T16793	nummod	CCGCTCGAGTCTCTACACTGTCACTCCCTG3,′
R11709	T16793	T16791	appos	′,′
R11710	T16794	T16795	punct	(,−
R11711	T16795	T16793	appos	−,′
R11712	T16796	T16795	nummod	634,−
R11713	T16797	T16796	prep	to,634
R11714	T16798	T16797	pobj	−,to
R11715	T16799	T16798	appos	605,−
R11716	T16800	T16798	punct	),−
R11717	T16801	T16802	punct	;,MHB1505
R11718	T16802	T16793	appos	MHB1505,′
R11719	T16803	T16802	punct	",",MHB1505
R11720	T16804	T16805	nummod	5,′
R11721	T16805	T16807	dep	′,′
R11722	T16806	T16807	nummod	CCGCTCGAGGGAGCCAAAAAAAGAATGC3,′
R11723	T16807	T16802	appos	′,MHB1505
R11724	T16808	T16809	punct	(,−
R11725	T16809	T16816	nmod	−,MHB1506
R11726	T16810	T16809	nummod	197,−
R11727	T16811	T16815	quantmod	to,;
R11728	T16812	T16811	pobj	−,to
R11729	T16813	T16812	appos	169,−
R11730	T16814	T16812	punct	),−
R11731	T16815	T16816	punct	;,MHB1506
R11732	T16816	T16830	nmod	MHB1506,MHB1532
R11733	T16817	T16816	punct	",",MHB1506
R11734	T16818	T16819	nummod	5,′
R11735	T16819	T16830	nmod	′,MHB1532
R11736	T16820	T16821	nummod	CCGCTCGAGCTGACCAATGGCTTCAAAG3,′
R11737	T16821	T16819	appos	′,′
R11738	T16822	T16823	punct	(,−
R11739	T16823	T16821	appos	−,′
R11740	T16824	T16823	nummod	85,−
R11741	T16825	T16824	prep	to,85
R11742	T16826	T16825	pobj	−,to
R11743	T16827	T16826	appos	58,−
R11744	T16828	T16826	punct	),−
R11745	T16829	T16830	punct	;,MHB1532
R11746	T16830	T16807	appos	MHB1532,′
R11747	T16831	T16830	punct	",",MHB1532
R11748	T16832	T16833	nummod	5,′
R11749	T16833	T16835	dep	′,′
R11750	T16834	T16835	nummod	CCGCTCGAGAATGCAGAACAAAGGGTCAGA3,′
R11751	T16835	T16830	appos	′,MHB1532
R11752	T16836	T16837	punct	(,−
R11753	T16837	T16835	appos	−,′
R11754	T16838	T16837	nummod	63,−
R11755	T16839	T16838	prep	to,63
R11756	T16840	T16839	pobj	−,to
R11757	T16841	T16840	appos	34,−
R11758	T16842	T16840	punct	),−
R11759	T16843	T16837	punct	;,−
R11760	T16844	T16837	cc	and,−
R11761	T16845	T16835	appos	MHB1507,′
R11762	T16846	T16845	punct	",",MHB1507
R11763	T16847	T16848	nummod	5,′
R11764	T16848	T16849	compound	′,CCGCTCGAGGTCTGCGAAGAATAAAAGGC
R11765	T16849	T16851	quantmod	CCGCTCGAGGTCTGCGAAGAATAAAAGGC,′
R11766	T16850	T16851	nummod	3,′
R11767	T16851	T16835	appos	′,′
R11768	T16852	T16853	punct	(,−
R11769	T16853	T16851	appos	−,′
R11770	T16854	T16853	dobj	37,−
R11771	T16855	T16853	prep	to,−
R11772	T16856	T16855	pobj	−,to
R11773	T16857	T16856	appos	9,−
R11774	T16858	T16856	punct	),−
R11775	T16859	T16772	punct	.,include
R11776	T16860	T16862	det	All,primers
R11777	T16861	T16862	amod	forward,primers
R11778	T16862	T16864	nsubjpass	primers,used
R11779	T16863	T16864	auxpass	were,used
R11780	T16864	T16864	ROOT	used,used
R11781	T16865	T16864	prep	in,used
R11782	T16866	T16865	pobj	combination,in
R11783	T16867	T16864	prep	with,used
R11784	T16868	T16872	det	the,site
R11785	T16869	T16870	compound	reverse,primer
R11786	T16870	T16872	compound	primer,site
R11787	T16871	T16872	compound	HindIII,site
R11788	T16872	T16867	pobj	site,with
R11789	T16873	T16872	punct	:,site
R11790	T16874	T16872	appos	MHB1477,site
R11791	T16875	T16874	punct	",",MHB1477
R11792	T16876	T16877	nummod	5,′
R11793	T16877	T16874	appos	′,MHB1477
R11794	T16878	T16879	nummod	CGGAAGCTTGGGAGGTTGCTGGTGA3,′
R11795	T16879	T16877	appos	′,′
R11796	T16880	T16881	punct	(,+45
R11797	T16881	T16879	appos	+45,′
R11798	T16882	T16881	prep	to,+45
R11799	T16883	T16882	pobj	+20,to
R11800	T16884	T16881	punct	),+45
R11801	T16885	T16864	punct	.,used
R11802	T16886	T16890	amod	Sox6-pcDNA3,]
R11803	T16887	T16890	nummod	.1,]
R11804	T16888	T16890	nmod	[,]
R11805	T16889	T16890	nummod	15,]
R11806	T16890	T16892	nsubjpass	],used
R11807	T16891	T16892	auxpass	was,used
R11808	T16892	T16892	ROOT	used,used
R11809	T16893	T16894	aux	to,overexpress
R11810	T16894	T16892	xcomp	overexpress,used
R11811	T16895	T16894	dobj	Sox6,overexpress
R11812	T16896	T16892	punct	.,used
R11813	T16897	T16899	det	A,version
R11877	T16961	T16960	nummod	63,−
R11878	T16962	T16961	prep	to,63
R11879	T16963	T16962	pobj	−,to
R11880	T16964	T16963	appos	19,−
R11881	T16965	T16963	punct	),−
R11882	T16966	T16967	punct	;,MHB1662
R11883	T16967	T16951	dobj	MHB1662,include
R11884	T16968	T16967	punct	",",MHB1662
R11885	T16969	T16970	nummod	5,′
R11886	T16970	T16986	nmod	′,CCGCTCGAGAATGCATGCCAAGGGTCAGATGAGTGTCTGCGAAGAA
R11887	T16971	T16972	nummod	CCGCTCGAGAATGCAGAACAAAGGGTCAGATGAGTGTCTGCGAAGAA3,′
R11888	T16972	T16970	appos	′,′
R11889	T16973	T16974	punct	(,−
R11890	T16974	T16972	appos	−,′
R11891	T16975	T16974	nummod	63,−
R11892	T16976	T16975	prep	to,63
R11893	T16977	T16976	pobj	−,to
R11894	T16978	T16977	appos	16,−
R11895	T16979	T16977	punct	),−
R11896	T16980	T16970	punct	;,′
R11897	T16981	T16970	cc	and,′
R11898	T16982	T16970	conj	MHB1663,′
R11899	T16983	T16982	punct	",",MHB1663
R11900	T16984	T16985	nummod	5,′
R11901	T16985	T16986	compound	′,CCGCTCGAGAATGCATGCCAAGGGTCAGATGAGTGTCTGCGAAGAA
R11902	T16986	T16967	conj	CCGCTCGAGAATGCATGCCAAGGGTCAGATGAGTGTCTGCGAAGAA,MHB1662
R11903	T16987	T16988	nummod	3,′
R11904	T16988	T16986	appos	′,CCGCTCGAGAATGCATGCCAAGGGTCAGATGAGTGTCTGCGAAGAA
R11905	T16989	T16990	punct	(,−
R11906	T16990	T16988	appos	−,′
R11907	T16991	T16990	nummod	63,−
R11908	T16992	T16990	prep	to,−
R11909	T16993	T16992	pobj	−,to
R11910	T16994	T16993	appos	18,−
R11911	T16995	T16993	punct	),−
R11912	T16996	T16951	punct	.,include
R12137	T17438	T17438	ROOT	Quantitation,Quantitation
R12138	T17439	T17438	prep	of,Quantitation
R12139	T17440	T17441	compound	globin,mRNA
R12140	T17441	T17439	pobj	mRNA,of
R12141	T17442	T17438	punct	.,Quantitation
R12142	T17443	T17444	nsubj	RNA,was
R12143	T17444	T17444	ROOT	was,was
R12144	T17445	T17446	amod	first,reverse
R12145	T17446	T17447	advmod	reverse,transcribed
R12146	T17447	T17444	acomp	transcribed,was
R12147	T17448	T17447	prep	to,transcribed
R12148	T17449	T17448	pobj	cDNA,to
R12149	T17450	T17444	punct	.,was
R12150	T17451	T17460	nsubjpass	Primers,obtained
R12151	T17452	T17451	prep	for,Primers
R12152	T17453	T17455	compound	cDNA,amplification
R12153	T17454	T17455	compound	PCR,amplification
R12154	T17455	T17452	pobj	amplification,for
R12155	T17456	T17455	prep	of,amplification
R12156	T17457	T17458	compound	globin,genes
R12157	T17458	T17456	pobj	genes,of
R12158	T17459	T17460	auxpass	were,obtained
R12159	T17460	T17460	ROOT	obtained,obtained
R12160	T17461	T17460	prep	from,obtained
R12161	T17462	T17463	compound	Primerbank,[
R12162	T17463	T17461	pobj	[,from
R12163	T17464	T17465	nummod	51,]
R12164	T17465	T17463	appos	],[
R12165	T17466	T17460	punct	.,obtained
R12166	T17467	T17468	det	All,primers
R12167	T17468	T17470	nsubjpass	primers,searched
R12168	T17469	T17470	auxpass	were,searched
R12169	T17470	T17470	ROOT	searched,searched
R12170	T17471	T17470	prep	against,searched
R12171	T17472	T17474	det	the,database
R12172	T17473	T17474	compound	NCBI,database
R12173	T17474	T17471	pobj	database,against
R12174	T17475	T17476	aux	to,confirm
R12175	T17476	T17470	advcl	confirm,searched
R12176	T17477	T17476	dobj	specificity,confirm
R12177	T17478	T17470	punct	.,searched
R12178	T17479	T17479	ROOT	For,For
R12179	T17480	T17481	amod	ɛy,globin
R12180	T17481	T17479	pobj	globin,For
R12181	T17482	T17481	punct	:,globin
R12182	T17483	T17496	nmod	MHB1666,′
R12183	T17484	T17483	punct	",",MHB1666
R12184	T17485	T17483	nummod	5,MHB1666
R12185	T17486	T17483	nummod	′,MHB1666
R12186	T17487	T17488	nummod	TGGCCTGTGGAGTAAGGTCAA3,′
R12187	T17488	T17488	ROOT	′,′
R12188	T17489	T17483	punct	;,MHB1666
R12189	T17490	T17483	cc	and,MHB1666
R12190	T17491	T17483	conj	MHB1667,MHB1666
R12191	T17492	T17491	punct	",",MHB1667
R12192	T17493	T17496	nummod	5,′
R12193	T17494	T17496	nummod	′,′
R12194	T17495	T17496	compound	GAAGCAGAGGACAAGTTCCCA3,′
R12195	T17496	T17481	appos	′,globin
R12196	T17497	T17479	punct	.,For
R12197	T17498	T17498	ROOT	For,For
R12198	T17499	T17500	amod	ζ,globin
R12199	T17500	T17498	pobj	globin,For
R12200	T17501	T17500	punct	:,globin
R12201	T17502	T17515	nummod	MHB1668,′
R12202	T17503	T17507	punct	",",′
R12203	T17504	T17507	nummod	5,′
R12204	T17505	T17507	nmod	′,′
R12205	T17506	T17507	nummod	CTACCCCCAGACGAAGACCTA3,′
R12206	T17507	T17502	meta	′,MHB1668
R12207	T17508	T17502	punct	;,MHB1668
R12208	T17509	T17502	cc	and,MHB1668
R12209	T17510	T17515	nmod	MHB1669,′
R12210	T17511	T17510	punct	",",MHB1669
R12211	T17512	T17513	nummod	5,′
R12212	T17513	T17515	nmod	′,′
R12213	T17514	T17515	nummod	CTTAACCGCATCCCCTACGG3,′
R12214	T17515	T17500	appos	′,globin
R12215	T17516	T17498	punct	.,For
R12216	T17517	T17517	ROOT	For,For
R12217	T17518	T17519	amod	βH1,globin
R12218	T17519	T17517	pobj	globin,For
R12219	T17520	T17519	punct	:,globin
R12220	T17521	T17534	nmod	MHB1672,′
R12221	T17522	T17521	punct	",",MHB1672
R12222	T17523	T17521	nummod	5,MHB1672
R12223	T17524	T17521	nummod	′,MHB1672
R12224	T17525	T17526	nummod	TGGACAACCTCAAGGAGACC3,′
R12225	T17526	T17526	ROOT	′,′
R12226	T17527	T17521	punct	;,MHB1672
R12227	T17528	T17521	cc	and,MHB1672
R12228	T17529	T17521	conj	MHB1673,MHB1672
R12229	T17530	T17529	punct	",",MHB1673
R12230	T17531	T17534	nummod	5,′
R12231	T17532	T17534	nummod	′,′
R12232	T17533	T17534	compound	ACCTCTGGGGTGAATTCCTT3,′
R12233	T17534	T17519	appos	′,globin
R12234	T17535	T17517	punct	.,For
R12235	T17536	T17536	ROOT	For,For
R12236	T17537	T17540	amod	βmaj,globin
R12237	T17538	T17540	compound	/,globin
R12238	T17539	T17540	compound	min,globin
R12239	T17540	T17536	pobj	globin,For
R12240	T17541	T17540	punct	:,globin
R12241	T17542	T17555	nmod	MHB1674,′
R12242	T17543	T17542	punct	:,MHB1674
R12243	T17544	T17547	nummod	5,′
R12244	T17545	T17547	nummod	′,′
R12245	T17546	T17547	compound	ATGGCCTGAATCACTTGGAC3,′
R12246	T17547	T17542	appos	′,MHB1674
R12247	T17548	T17542	punct	;,MHB1674
R12248	T17549	T17542	cc	and,MHB1674
R12249	T17550	T17542	conj	MHB1675,MHB1674
R12250	T17551	T17550	punct	",",MHB1675
R12251	T17552	T17555	nummod	5,′
R12252	T17553	T17555	nummod	′,′
R12253	T17554	T17555	compound	ACGATCATATTGCCCAGGAG3,′
R12254	T17555	T17540	appos	′,globin
R12255	T17556	T17536	punct	.,For
R12256	T17557	T17579	advcl	Using,run
R12257	T17558	T17562	det	the,kit
R12258	T17559	T17562	nmod	SYBR,kit
R12259	T17560	T17562	amod	green,kit
R12260	T17561	T17562	compound	supermix,kit
R12261	T17562	T17557	dobj	kit,Using
R12262	T17563	T17557	prep	with,Using
R12263	T17564	T17563	pobj	ROX,with
R12264	T17565	T17566	punct	(,Bio-Rad
R12265	T17566	T17564	appos	Bio-Rad,ROX
R12266	T17567	T17566	punct	",",Bio-Rad
R12267	T17568	T17566	conj	Hercules,Bio-Rad
R12268	T17569	T17568	punct	",",Hercules
R12269	T17570	T17568	conj	California,Hercules
R12270	T17571	T17570	punct	",",California
R12271	T17572	T17573	compound	United,States
R12272	T17573	T17570	conj	States,California
R12273	T17574	T17570	punct	),California
R12274	T17575	T17579	punct	",",run
R12275	T17576	T17577	compound	PCR,amplification
R12276	T17577	T17579	nsubjpass	amplification,run
R12277	T17578	T17579	auxpass	was,run
R12278	T17579	T17579	ROOT	run,run
R12279	T17580	T17579	prep	on,run
R12280	T17581	T17585	det	an,Biosystems
R12281	T17582	T17585	nmod	ABI7000,Biosystems
R12282	T17583	T17585	punct	(,Biosystems
R12283	T17584	T17585	compound	Applied,Biosystems
R12284	T17585	T17580	pobj	Biosystems,on
R12285	T17586	T17585	punct	",",Biosystems
R12286	T17587	T17588	compound	Foster,City
R12287	T17588	T17585	conj	City,Biosystems
R12288	T17589	T17588	punct	",",City
R12289	T17590	T17588	appos	California,City
R12290	T17591	T17590	punct	",",California
R12291	T17592	T17593	compound	United,States
R12292	T17593	T17590	appos	States,California
R12293	T17594	T17588	punct	),City
R12294	T17595	T17585	prep	at,Biosystems
R12295	T17596	T17597	det	the,University
R12296	T17597	T17601	nmod	University,facility
R12297	T17598	T17597	prep	of,University
R12298	T17599	T17598	pobj	Arizona,of
R12299	T17600	T17601	amod	core,facility
R12300	T17601	T17595	pobj	facility,at
R12301	T17602	T17579	punct	.,run
R12302	T17603	T17604	det	All,PCR
R12303	T17604	T17606	nsubjpass	PCR,performed
R12304	T17605	T17606	auxpass	was,performed
R12305	T17606	T17606	ROOT	performed,performed
R12306	T17607	T17606	prep	in,performed
R12307	T17608	T17610	det	a,reaction
R12308	T17609	T17610	amod	25-μl,reaction
R12309	T17610	T17607	pobj	reaction,in
R12310	T17611	T17610	prep	with,reaction
R12311	T17612	T17613	nummod	12.5,μl
R12312	T17613	T17611	pobj	μl,with
R12313	T17614	T17616	nmod	SYBR,supermix
R12314	T17615	T17616	amod	green,supermix
R12315	T17616	T17613	appos	supermix,μl
R12316	T17617	T17606	punct	.,performed
R12317	T17618	T17619	compound	GAPDH,mRNA
R12318	T17619	T17620	compound	mRNA,levels
R12319	T17620	T17622	nsubjpass	levels,used
R12320	T17621	T17622	auxpass	were,used
R12321	T17622	T17622	ROOT	used,used
R12322	T17623	T17622	prep	as,used
R12323	T17624	T17623	pobj	control,as
R12324	T17625	T17624	prep	for,control
R12325	T17626	T17627	compound	input,RNA
R12326	T17627	T17625	pobj	RNA,for
R12327	T17628	T17622	punct	.,used
R12328	T17629	T17631	compound	Standard,analyses
R12329	T17630	T17631	compound	curve,analyses
R12330	T17631	T17633	nsubjpass	analyses,performed
R12331	T17632	T17633	auxpass	were,performed
R12332	T17633	T17633	ROOT	performed,performed
R12333	T17634	T17635	aux	to,test
R12334	T17635	T17633	xcomp	test,performed
R12335	T17636	T17637	det	the,efficiency
R12336	T17637	T17635	dobj	efficiency,test
R12337	T17638	T17637	prep	of,efficiency
R12338	T17639	T17640	det	the,amplifications
R12339	T17640	T17638	pobj	amplifications,of
R12340	T17641	T17633	punct	.,performed
R12341	T17642	T17644	nsubjpass	Triplicates,done
R12342	T17643	T17644	auxpass	were,done
R12343	T17644	T17644	ROOT	done,done
R12344	T17645	T17644	prep	for,done
R12345	T17646	T17648	det	each,reaction
R12346	T17647	T17648	compound	PCR,reaction
R12347	T17648	T17645	pobj	reaction,for
R12348	T17649	T17644	punct	.,done
R12349	T17650	T17652	amod	Relative,values
R12350	T17651	T17652	amod	quantitative,values
R12351	T17652	T17654	nsubjpass	values,calculated
R12352	T17653	T17654	auxpass	were,calculated
R12353	T17654	T17654	ROOT	calculated,calculated
R12354	T17655	T17654	prep	in,calculated
R12355	T17656	T17661	det	the,Software
R12356	T17657	T17658	compound	ABI,Prism
R12357	T17658	T17661	nmod	Prism,Software
R12358	T17659	T17661	nummod	7000,Software
R12359	T17660	T17661	compound	SDS,Software
R12360	T17661	T17655	pobj	Software,in
R12361	T17662	T17664	punct	(,Biosystems
R12362	T17663	T17664	compound	Applied,Biosystems
R12363	T17664	T17661	appos	Biosystems,Software
R12364	T17665	T17664	punct	),Biosystems
R12365	T17666	T17654	cc	and,calculated
R12366	T17667	T17654	conj	normalized,calculated
R12367	T17668	T17667	prep	to,normalized
R12368	T17669	T17668	pobj	GAPDH,to
R12369	T17670	T17667	prep	in,normalized
R12370	T17671	T17672	compound	Microsoft,Excel
R12371	T17672	T17670	pobj	Excel,in
R12372	T17673	T17674	punct	(,Redmond
R12373	T17674	T17672	appos	Redmond,Excel
R12374	T17675	T17674	punct	",",Redmond
R12375	T17676	T17674	conj	Washington,Redmond
R12376	T17677	T17676	punct	",",Washington
R12377	T17678	T17679	compound	United,States
R12378	T17679	T17674	appos	States,Redmond
R12379	T17680	T17674	punct	),Redmond
R12380	T17681	T17654	punct	.,calculated
R12582	T17981	T17979	dep	549,nucleotides
R12583	T17982	T17979	punct	;,nucleotides
R12584	T17983	T17979	cc	and,nucleotides
R12585	T17984	T17986	nmod	mouse,nucleotides
R12586	T17985	T17986	compound	Sox6,nucleotides
R12587	T17986	T17979	conj	nucleotides,nucleotides
R12588	T17987	T17979	dep	1353,nucleotides
R12589	T17988	T17979	appos	1927,nucleotides
R12590	T17989	T17977	punct	.,βmaj
R12591	T17990	T17992	nsubjpass	Embryos,fixed
R12592	T17991	T17992	auxpass	were,fixed
R12593	T17992	T17992	ROOT	fixed,fixed
R12594	T17993	T17992	advmod	overnight,fixed
R12595	T17994	T17992	agent	by,fixed
R12596	T17995	T17994	pobj	immersion,by
R12597	T17996	T17992	prep	in,fixed
R12598	T17997	T17998	nummod	4,%
R12599	T17998	T17999	compound	%,paraformaldehyde
R12600	T17999	T17996	pobj	paraformaldehyde,in
R12601	T18000	T17992	punct	",",fixed
R12602	T18001	T17992	conj	embedded,fixed
R12603	T18002	T18001	prep	in,embedded
R12604	T18003	T18002	pobj	paraffin,in
R12605	T18004	T17992	punct	",",fixed
R12606	T18005	T17992	conj	sectioned,fixed
R12607	T18006	T18005	prep	at,sectioned
R12608	T18007	T18008	nummod	5,μm
R12609	T18008	T18006	pobj	μm,at
R12610	T18009	T17992	punct	",",fixed
R12611	T18010	T17992	cc	and,fixed
R12612	T18011	T17992	conj	adhered,fixed
R12613	T18012	T18013	aux	to,charge
R12614	T18013	T18011	xcomp	charge,adhered
R12615	T18014	T18015	amod	modified,slides
R12616	T18015	T18013	dobj	slides,charge
R12617	T18016	T18017	punct	(,VWR
R12618	T18017	T18015	appos	VWR,slides
R12619	T18018	T18017	punct	",",VWR
R12620	T18019	T18020	compound	West,Chester
R12621	T18020	T18017	conj	Chester,VWR
R12622	T18021	T18020	punct	",",Chester
R12623	T18022	T18020	conj	Pennsylvania,Chester
R12624	T18023	T18022	punct	",",Pennsylvania
R12625	T18024	T18025	compound	United,States
R12626	T18025	T18022	conj	States,Pennsylvania
R12627	T18026	T18015	punct	),slides
R12628	T18027	T17992	punct	.,fixed
R12629	T18028	T18030	nsubjpass	Slides,processed
R12630	T18029	T18030	auxpass	were,processed
R12631	T18030	T18030	ROOT	processed,processed
R12632	T18031	T18030	prep	for,processed
R12633	T18032	T18030	prep	in,processed
R12634	T18033	T18034	compound	situ,hybridization
R12635	T18034	T18032	pobj	hybridization,in
R12636	T18035	T18036	mark	as,described
R12637	T18036	T18030	advcl	described,processed
R12638	T18037	T18039	nmod	[,]
R12639	T18038	T18039	nummod	52,]
R12640	T18039	T18036	dobj	],described
R12641	T18040	T18036	advcl	using,described
R12642	T18041	T18040	prep	in,using
R12643	T18042	T18041	pobj	vitro,in
R12644	T18043	T18045	amod	transcribed,probes
R12645	T18044	T18045	compound	RNA,probes
R12646	T18045	T18040	dobj	probes,using
R12647	T18046	T18045	acl	labeled,probes
R12648	T18047	T18046	prep	with,labeled
R12649	T18048	T18047	pobj	33P,with
R12650	T18049	T18030	punct	.,processed
R12651	T18050	T18053	nmod	Darkfield,images
R12652	T18051	T18050	cc	and,Darkfield
R12653	T18052	T18050	conj	brightfield,Darkfield
R12654	T18053	T18055	nsubjpass	images,obtained
R12655	T18054	T18055	auxpass	were,obtained
R12656	T18055	T18055	ROOT	obtained,obtained
R12657	T18056	T18055	prep	with,obtained
R12658	T18057	T18060	det	a,microscope
R12659	T18058	T18060	compound	Nikon,microscope
R12660	T18059	T18060	compound	Optiphot,microscope
R12661	T18060	T18056	pobj	microscope,with
R12662	T18061	T18062	punct	(,Nikon
R12663	T18062	T18076	nmod	Nikon,camera
R12664	T18063	T18062	punct	",",Nikon
R12665	T18064	T18062	conj	Melville,Nikon
R12666	T18065	T18064	punct	",",Melville
R12667	T18066	T18067	compound	New,York
R12668	T18067	T18064	conj	York,Melville
R12669	T18068	T18067	punct	",",York
R12670	T18069	T18070	compound	United,States
R12671	T18070	T18067	conj	States,York
R12672	T18071	T18070	punct	),States
R12673	T18072	T18070	cc	and,States
R12674	T18073	T18076	nmod	SPOT,camera
R12675	T18074	T18076	amod	RT-Slider,camera
R12676	T18075	T18076	amod	digital,camera
R12677	T18076	T18060	appos	camera,microscope
R12678	T18077	T18079	punct	(,Instruments
R12679	T18078	T18079	compound	Diagnostic,Instruments
R12680	T18079	T18076	appos	Instruments,camera
R12681	T18080	T18079	punct	",",Instruments
R12682	T18081	T18082	compound	Sterling,Heights
R12683	T18082	T18079	conj	Heights,Instruments
R12684	T18083	T18082	punct	",",Heights
R12685	T18084	T18082	conj	Michigan,Heights
R12686	T18085	T18084	punct	",",Michigan
R12687	T18086	T18087	compound	United,States
R12688	T18087	T18084	conj	States,Michigan
R12689	T18088	T18079	punct	),Instruments
R12690	T18089	T18055	punct	.,obtained
R12691	T18090	T18092	nsubj	Objectives,were
R12692	T18091	T18090	acl	used,Objectives
R12693	T18092	T18092	ROOT	were,were
R12694	T18093	T18094	nummod	1,×
R12695	T18094	T18092	attr	×,were
R12696	T18095	T18098	punct	(,0.04
R12697	T18096	T18098	dep	NA,0.04
R12698	T18097	T18098	punct	=,0.04
R12699	T18098	T18094	parataxis	0.04,×
R12700	T18099	T18098	punct	),0.04
R12701	T18100	T18098	cc	and,0.04
R12702	T18101	T18102	nummod	10,×
R12703	T18102	T18098	conj	×,0.04
R12704	T18103	T18102	punct	(,×
R12705	T18104	T18107	nmod	NA,)
R12706	T18105	T18106	punct	=,0.5
R12707	T18106	T18104	nummod	0.5,NA
R12708	T18107	T18102	punct	),×
R12709	T18108	T18092	punct	.,were
R12710	T18109	T18111	nsubjpass	Images,processed
R12711	T18110	T18111	auxpass	were,processed
R12712	T18111	T18111	ROOT	processed,processed
R12713	T18112	T18111	punct	",",processed
R12714	T18113	T18111	conj	pseudocolored,processed
R12715	T18114	T18113	punct	",",pseudocolored
R12716	T18115	T18113	cc	and,pseudocolored
R12717	T18116	T18113	conj	combined,pseudocolored
R12718	T18117	T18116	advcl	using,combined
R12719	T18118	T18117	dobj	Photoshop,using
R12720	T18119	T18120	punct	(,Adobe
R12721	T18120	T18118	appos	Adobe,Photoshop
R12722	T18121	T18120	punct	",",Adobe
R12723	T18122	T18123	compound	San,Jose
R12724	T18123	T18120	npadvmod	Jose,Adobe
R12725	T18124	T18123	punct	",",Jose
R12726	T18125	T18123	conj	California,Jose
R12727	T18126	T18125	punct	",",California
R12728	T18127	T18128	compound	United,States
R12729	T18128	T18125	appos	States,California
R12730	T18129	T18125	punct	),California
R12731	T18130	T18118	conj	software,Photoshop
R12732	T18131	T18117	prep	with,using
R12733	T18132	T18136	nmod	Fovea,Graphics
R12734	T18133	T18136	nmod	Pro,Graphics
R12735	T18134	T18136	punct	(,Graphics
R12736	T18135	T18136	compound	Reindeer,Graphics
R12737	T18136	T18146	nmod	Graphics,plugins
R12738	T18137	T18136	punct	",",Graphics
R12739	T18138	T18146	nmod	Asheville,plugins
R12740	T18139	T18138	punct	",",Asheville
R12741	T18140	T18141	compound	North,Carolina
R12742	T18141	T18138	conj	Carolina,Asheville
R12743	T18142	T18141	punct	",",Carolina
R12744	T18143	T18144	compound	United,States
R12745	T18144	T18141	appos	States,Carolina
R12746	T18145	T18146	punct	),plugins
R12747	T18146	T18131	pobj	plugins,with
R12748	T18147	T18111	punct	.,processed
R12749	T18148	T18149	amod	Original,images
R12750	T18149	T18150	nsubj	images,are
R12751	T18150	T18150	ROOT	are,are
R12752	T18151	T18150	acomp	available,are
R12753	T18152	T18150	punct	.,are
R12873	T18304	T18304	ROOT	Histology,Histology
R12874	T18305	T18304	punct	.,Histology
R12875	T18306	T18307	amod	18.5-dpc,embryos
R12876	T18307	T18309	nsubjpass	embryos,exsanguinated
R12877	T18308	T18309	auxpass	were,exsanguinated
R12878	T18309	T18309	ROOT	exsanguinated,exsanguinated
R12879	T18310	T18309	cc	and,exsanguinated
R12880	T18311	T18309	conj	peripheral,exsanguinated
R12881	T18312	T18313	compound	blood,smears
R12882	T18313	T18314	nsubj	smears,were
R12883	T18314	T18315	auxpass	were,prepared
R12884	T18315	T18309	conj	prepared,exsanguinated
R12885	T18316	T18315	prep	from,prepared
R12886	T18317	T18318	preconj	both,mutant
R12887	T18318	T18321	amod	mutant,mice
R12888	T18319	T18318	cc	and,mutant
R12889	T18320	T18318	conj	WT,mutant
R12890	T18321	T18316	pobj	mice,from
R12891	T18322	T18309	punct	.,exsanguinated
R12892	T18323	T18324	det	The,slides
R12893	T18324	T18325	nsubj	slides,were
R12894	T18325	T18325	ROOT	were,were
R12895	T18326	T18325	acomp	Wright-stained,were
R12896	T18327	T18325	cc	and,were
R12897	T18328	T18325	conj	read,were
R12898	T18329	T18328	agent	by,read
R12899	T18330	T18329	pobj	DAF,by
R12900	T18331	T18325	punct	.,were
R12901	T18332	T18349	prep	For,fixed
R12902	T18333	T18335	amod	whole,analysis
R12903	T18334	T18335	compound	mount,analysis
R12904	T18335	T18332	pobj	analysis,For
R12905	T18336	T18349	punct	",",fixed
R12906	T18337	T18338	compound	14.5-dpc,WT
R12907	T18338	T18349	nsubjpass	WT,fixed
R12908	T18339	T18338	cc	and,WT
R12909	T18340	T18341	amod	mutant,embryos
R12910	T18341	T18338	conj	embryos,WT
R12911	T18342	T18341	punct	",",embryos
R12912	T18343	T18341	cc	and,embryos
R12913	T18344	T18345	amod	postnatal,day
R12914	T18345	T18349	npadvmod	day,fixed
R12915	T18346	T18347	nummod	10.5,mice
R12916	T18347	T18349	nsubjpass	mice,fixed
R12917	T18348	T18349	auxpass	were,fixed
R12918	T18349	T18349	ROOT	fixed,fixed
R12919	T18350	T18349	prep	in,fixed
R12920	T18351	T18352	nummod	10,%
R12921	T18352	T18353	compound	%,formalin
R12922	T18353	T18350	pobj	formalin,in
R12923	T18354	T18353	punct	",",formalin
R12924	T18355	T18357	amod	paraffin-embedded,sectioned
R12925	T18356	T18355	punct	",",paraffin-embedded
R12926	T18357	T18349	conj	sectioned,fixed
R12927	T18358	T18357	prep	at,sectioned
R12928	T18359	T18360	nummod	5,μm
R12929	T18360	T18358	pobj	μm,at
R12930	T18361	T18357	punct	",",sectioned
R12931	T18362	T18357	cc	and,sectioned
R12932	T18363	T18357	conj	stained,sectioned
R12933	T18364	T18363	prep	with,stained
R12934	T18365	T18364	pobj	hematoxylin,with
R12935	T18366	T18365	cc	and,hematoxylin
R12936	T18367	T18365	conj	eosin,hematoxylin
R12937	T18368	T18349	punct	.,fixed
R12938	T18369	T18370	compound	Liver,samples
R12939	T18370	T18380	nsubjpass	samples,prepared
R12940	T18371	T18372	punct	(,at
R12941	T18372	T18370	prep	at,samples
R12942	T18373	T18372	pobj	14.5,at
R12943	T18374	T18380	nsubjpass	dpc,prepared
R12944	T18375	T18374	cc	and,dpc
R12945	T18376	T18377	nummod	18.5,dpc
R12946	T18377	T18374	conj	dpc,dpc
R12947	T18378	T18374	punct	),dpc
R12948	T18379	T18380	auxpass	were,prepared
R12949	T18380	T18380	ROOT	prepared,prepared
R12950	T18381	T18380	prep	in,prepared
R12951	T18382	T18384	det	a,manner
R12952	T18383	T18384	amod	similar,manner
R12953	T18384	T18381	pobj	manner,in
R12954	T18385	T18380	punct	.,prepared
R12955	T18386	T18388	nsubjpass	Images,obtained
R12956	T18387	T18388	auxpass	were,obtained
R12957	T18388	T18388	ROOT	obtained,obtained
R12958	T18389	T18388	prep	with,obtained
R12959	T18390	T18392	compound	Nikon,microscope
R12960	T18391	T18392	compound	Labophot-2,microscope
R12961	T18392	T18389	pobj	microscope,with
R12962	T18393	T18388	punct	.,obtained
R12963	T18394	T18396	nsubj	Objectives,were
R12964	T18395	T18394	acl	used,Objectives
R12965	T18396	T18396	ROOT	were,were
R12966	T18397	T18398	compound	E,Plan
R12967	T18398	T18396	attr	Plan,were
R12968	T18399	T18398	nummod	40/0,Plan
R12969	T18400	T18398	nummod	.65,Plan
R12970	T18401	T18398	appos	160/0,Plan
R12971	T18402	T18403	nummod	.17,Nikon
R12972	T18403	T18398	conj	Nikon,Plan
R12973	T18404	T18403	punct	(,Nikon
R12974	T18405	T18406	nummod	40,×
R12975	T18406	T18407	compound	×,objective
R12976	T18407	T18403	appos	objective,Nikon
R12977	T18408	T18403	punct	),Nikon
R12978	T18409	T18403	punct	",",Nikon
R12979	T18410	T18411	compound	E,Plan
R12980	T18411	T18398	conj	Plan,Plan
R12981	T18412	T18411	nummod	100/1,Plan
R12982	T18413	T18398	appos	.25,Plan
R12983	T18414	T18415	compound	oil,160/0
R12984	T18415	T18417	nmod	160/0,Nikon
R12985	T18416	T18417	nummod	.17,Nikon
R12986	T18417	T18398	conj	Nikon,Plan
R12987	T18418	T18417	punct	(,Nikon
R12988	T18419	T18421	nummod	100,objective
R12989	T18420	T18421	nummod	×,objective
R12990	T18421	T18417	appos	objective,Nikon
R12991	T18422	T18417	punct	),Nikon
R12992	T18423	T18396	punct	.,were
R12993	T18424	T18425	det	The,camera
R12994	T18425	T18426	nsubj	camera,was
R12995	T18426	T18426	ROOT	was,was
R12996	T18427	T18429	det	a,Coolpix
R12997	T18428	T18429	compound	Nikon,Coolpix
R12998	T18429	T18430	compound	Coolpix,4300
R12999	T18430	T18426	attr	4300,was
R13000	T18431	T18426	punct	.,was
R13001	T18432	T18433	amod	Original,images
R13002	T18433	T18434	nsubj	images,are
R13003	T18434	T18434	ROOT	are,are
R13004	T18435	T18434	acomp	available,are
R13005	T18436	T18434	punct	.,are
R13112	T18572	T18573	compound	Northern,blot
R13113	T18573	T18573	ROOT	blot,blot
R13114	T18574	T18573	punct	.,blot
R13115	T18575	T18581	det	A,filter
R13116	T18576	T18581	compound	mouse,filter
R13117	T18577	T18578	amod	embryonic,tissue
R13118	T18578	T18581	compound	tissue,filter
R13119	T18579	T18580	compound	Northern,blot
R13120	T18580	T18581	compound	blot,filter
R13121	T18581	T18593	nsubjpass	filter,hybridized
R13122	T18582	T18581	punct	(,filter
R13123	T18583	T18581	appos	Seegene,filter
R13124	T18584	T18583	punct	",",Seegene
R13125	T18585	T18583	conj	Rockville,Seegene
R13126	T18586	T18585	punct	",",Rockville
R13127	T18587	T18585	conj	Maryland,Rockville
R13128	T18588	T18587	punct	",",Maryland
R13129	T18589	T18590	compound	United,States
R13130	T18590	T18587	appos	States,Maryland
R13131	T18591	T18587	punct	),Maryland
R13132	T18592	T18593	auxpass	was,hybridized
R13133	T18593	T18593	ROOT	hybridized,hybridized
R13134	T18594	T18593	prep	with,hybridized
R13135	T18595	T18597	det	a,probe
R13136	T18596	T18597	amod	Sox6,probe
R13137	T18597	T18594	pobj	probe,with
R13138	T18598	T18597	acl	generated,probe
R13139	T18599	T18598	agent	by,generated
R13140	T18600	T18599	pobj	RT-PCR,by
R13141	T18601	T18602	punct	(,nucleotides
R13142	T18602	T18597	appos	nucleotides,probe
R13143	T18603	T18602	nummod	1353,nucleotides
R13144	T18604	T18602	npadvmod	1927,nucleotides
R13145	T18605	T18602	punct	),nucleotides
R13146	T18606	T18593	cc	and,hybridized
R13147	T18607	T18593	conj	labeled,hybridized
R13148	T18608	T18607	prep	with,labeled
R13149	T18609	T18612	compound	[,dCTP
R13150	T18610	T18612	compound	α-32P,dCTP
R13151	T18611	T18612	compound	],dCTP
R13152	T18612	T18608	pobj	dCTP,with
R13153	T18613	T18612	punct	",",dCTP
R13154	T18614	T18607	agent	by,labeled
R13155	T18615	T18616	amod	random,primer
R13156	T18616	T18614	pobj	primer,by
R13157	T18617	T18616	acl	labeling,primer
R13158	T18618	T18619	punct	(,RediprimeII
R13159	T18619	T18617	ccomp	RediprimeII,labeling
R13160	T18620	T18619	punct	;,RediprimeII
R13161	T18621	T18622	compound	Amersham,Biosciences
R13162	T18622	T18619	conj	Biosciences,RediprimeII
R13163	T18623	T18622	punct	",",Biosciences
R13164	T18624	T18622	conj	Buckinghamshire,Biosciences
R13165	T18625	T18624	punct	",",Buckinghamshire
R13166	T18626	T18624	conj	England,Buckinghamshire
R13167	T18627	T18626	punct	",",England
R13168	T18628	T18629	compound	United,Kingdom
R13169	T18629	T18619	npadvmod	Kingdom,RediprimeII
R13170	T18630	T18619	punct	),RediprimeII
R13171	T18631	T18593	punct	.,hybridized
R13172	T18632	T18633	det	The,hybridization
R13173	T18633	T18635	nsubjpass	hybridization,performed
R13174	T18634	T18635	auxpass	was,performed
R13175	T18635	T18635	ROOT	performed,performed
R13176	T18636	T18635	prep	in,performed
R13177	T18637	T18636	pobj	phosphate,in
R13178	T18638	T18635	advcl	buffered,performed
R13179	T18639	T18640	nummod	7,%
R13180	T18640	T18643	compound	%,solution
R13181	T18641	T18643	compound	SDS,solution
R13182	T18642	T18643	compound	hybridization,solution
R13183	T18643	T18638	dobj	solution,buffered
R13184	T18644	T18635	punct	.,performed
R13185	T18645	T18647	nsubjpass	Blots,washed
R13186	T18646	T18647	auxpass	were,washed
R13187	T18647	T18647	ROOT	washed,washed
R13188	T18648	T18647	prep	with,washed
R13189	T18649	T18651	nummod	0.2,SSC
R13190	T18650	T18651	nummod	×,SSC
R13191	T18651	T18648	pobj	SSC,with
R13192	T18652	T18651	punct	",",SSC
R13193	T18653	T18654	nummod	1,%
R13194	T18654	T18655	compound	%,SDS
R13195	T18655	T18651	appos	SDS,SSC
R13196	T18656	T18647	prep	at,washed
R13197	T18657	T18658	nummod	60,°
R13198	T18658	T18656	pobj	°,at
R13199	T18659	T18660	compound	C,prior
R13200	T18660	T18647	advmod	prior,washed
R13201	T18661	T18660	prep	to,prior
R13202	T18662	T18661	pobj	exposure,to
R13203	T18663	T18662	prep	to,exposure
R13204	T18664	T18665	compound	X-ray,film
R13205	T18665	T18663	pobj	film,to
R13206	T18666	T18667	punct	(,Kodak
R13207	T18667	T18665	appos	Kodak,film
R13208	T18668	T18667	punct	",",Kodak
R13209	T18669	T18667	conj	Rochester,Kodak
R13210	T18670	T18669	punct	",",Rochester
R13211	T18671	T18672	compound	New,York
R13212	T18672	T18669	conj	York,Rochester
R13213	T18673	T18672	punct	",",York
R13214	T18674	T18675	compound	United,States
R13215	T18675	T18672	appos	States,York
R13216	T18676	T18672	punct	),York
R13217	T18677	T18660	prep	at,prior
R13218	T18678	T18680	nummod	−,°
R13219	T18679	T18680	nummod	80,°
R13220	T18680	T18677	pobj	°,at
R13221	T18681	T18660	npadvmod	C,prior
R13222	T18682	T18660	prep	for,prior
R13223	T18683	T18684	nummod	6,d.
R13224	T18684	T18682	pobj	d.,for
R13398	T18901	T18902	compound	Cell,culture
R13399	T18902	T18902	ROOT	culture,culture
R13400	T18903	T18902	cc	and,culture
R13401	T18904	T18902	conj	transfection,culture
R13402	T18905	T18902	punct	.,culture
R13403	T18906	T18907	nummod	GM979,cells
R13404	T18907	T18922	nsubjpass	cells,cultured
R13405	T18908	T18907	punct	(,cells
R13406	T18909	T18911	compound	Coriell,Repositories
R13407	T18910	T18911	compound	Cell,Repositories
R13408	T18911	T18907	appos	Repositories,cells
R13409	T18912	T18911	punct	",",Repositories
R13410	T18913	T18911	conj	Camden,Repositories
R13411	T18914	T18913	punct	",",Camden
R13412	T18915	T18916	compound	New,Jersey
R13413	T18916	T18913	conj	Jersey,Camden
R13414	T18917	T18916	punct	",",Jersey
R13415	T18918	T18919	compound	United,States
R13416	T18919	T18916	appos	States,Jersey
R13417	T18920	T18916	punct	),Jersey
R13418	T18921	T18922	auxpass	were,cultured
R13419	T18922	T18922	ROOT	cultured,cultured
R13420	T18923	T18922	prep	in,cultured
R13421	T18924	T18926	poss	Ham,F12
R13422	T18925	T18924	case	's,Ham
R13423	T18926	T18923	pobj	F12,in
R13424	T18927	T18931	mark	with,supplemented
R13425	T18928	T18930	nummod	2,L-glutamine
R13426	T18929	T18930	compound	mM,L-glutamine
R13427	T18930	T18931	nsubj	L-glutamine,supplemented
R13428	T18931	T18922	advcl	supplemented,cultured
R13429	T18932	T18931	prep	with,supplemented
R13430	T18933	T18938	amod	heat-inactivated,serum
R13431	T18934	T18935	nummod	10,%
R13432	T18935	T18938	compound	%,serum
R13433	T18936	T18937	amod	fetal,calf
R13434	T18937	T18938	compound	calf,serum
R13435	T18938	T18932	pobj	serum,with
R13436	T18939	T18938	punct	(,serum
R13437	T18940	T18968	nmod	Ivitrogen,)
R13438	T18941	T18940	punct	",",Ivitrogen
R13439	T18942	T18940	conj	Carlsbad,Ivitrogen
R13440	T18943	T18942	punct	",",Carlsbad
R13441	T18944	T18942	conj	California,Carlsbad
R13442	T18945	T18944	punct	",",California
R13443	T18946	T18947	compound	United,States
R13444	T18947	T18944	conj	States,California
R13445	T18948	T18944	punct	),California
R13446	T18949	T18940	punct	",",Ivitrogen
R13447	T18950	T18967	nmod	penicillin,mM
R13448	T18951	T18953	punct	(,units/ml
R13449	T18952	T18953	nummod	100,units/ml
R13450	T18953	T18950	appos	units/ml,penicillin
R13451	T18954	T18953	punct	),units/ml
R13452	T18955	T18950	punct	",",penicillin
R13453	T18956	T18960	nmod	streptomycin,ml
R13454	T18957	T18956	nummod	100,streptomycin
R13455	T18958	T18960	compound	μg,ml
R13456	T18959	T18960	compound	/,ml
R13457	T18960	T18950	appos	ml,penicillin
R13458	T18961	T18960	punct	),ml
R13459	T18962	T18950	punct	",",penicillin
R13460	T18963	T18950	cc	and,penicillin
R13461	T18964	T18950	conj	L-glutamine,penicillin
R13462	T18965	T18966	punct	(,2
R13463	T18966	T18967	nummod	2,mM
R13464	T18967	T18940	appos	mM,Ivitrogen
R13465	T18968	T18938	punct	),serum
R13466	T18969	T18922	punct	.,cultured
R13467	T18970	T18971	compound	MEL,cells
R13468	T18971	T18976	nsubjpass	cells,supplemented
R13469	T18972	T18976	auxpass	were,supplemented
R13470	T18973	T18972	acomp	cultured,were
R13471	T18974	T18973	prep	in,cultured
R13472	T18975	T18974	pobj	DMEM,in
R13473	T18976	T18976	ROOT	supplemented,supplemented
R13474	T18977	T18976	prep	as,supplemented
R13475	T18978	T18977	prep	above,as
R13476	T18979	T18978	punct	(,above
R13477	T18980	T18978	prep	without,above
R13478	T18981	T18980	pobj	heat,without
R13479	T18982	T18978	pcomp	inactivating,above
R13480	T18983	T18984	det	the,serum
R13481	T18984	T18982	dobj	serum,inactivating
R13482	T18985	T18977	punct	),as
R13483	T18986	T18976	punct	.,supplemented
R13484	T18987	T18988	nummod	GM979,cells
R13485	T18988	T19000	nsubjpass	cells,transfected
R13486	T18989	T18988	punct	(,cells
R13487	T18990	T18992	nummod	4,105
R13488	T18991	T18992	nummod	×,105
R13489	T18992	T18988	appos	105,cells
R13490	T18993	T18988	punct	),cells
R13491	T18994	T18988	prep	in,cells
R13492	T18995	T18996	compound	log,phase
R13493	T18996	T18994	pobj	phase,in
R13494	T18997	T18996	prep	of,phase
R13495	T18998	T18997	pobj	growth,of
R13496	T18999	T19000	auxpass	were,transfected
R13497	T19000	T19000	ROOT	transfected,transfected
R13498	T19001	T19000	prep	with,transfected
R13499	T19002	T19001	pobj	plasmids,with
R13500	T19003	T19000	agent	by,transfected
R13501	T19004	T19003	pobj	FuGENE6,by
R13502	T19005	T19006	punct	(,Roche
R13503	T19006	T19004	appos	Roche,FuGENE6
R13504	T19007	T19006	punct	",",Roche
R13505	T19008	T19013	nmod	Indianapolis,States
R13506	T19009	T19008	punct	",",Indianapolis
R13507	T19010	T19008	conj	Indiana,Indianapolis
R13508	T19011	T19010	punct	",",Indiana
R13509	T19012	T19013	compound	United,States
R13510	T19013	T19006	conj	States,Roche
R13511	T19014	T19006	punct	),Roche
R13512	T19015	T19000	punct	.,transfected
R13513	T19016	T19018	nsubjpass	Cells,transfected
R13514	T19017	T19018	auxpass	were,transfected
R13515	T19018	T19018	ROOT	transfected,transfected
R13516	T19019	T19018	prep	with,transfected
R13517	T19020	T19022	amod	ɛy,reporter
R13518	T19021	T19022	compound	promoter,reporter
R13519	T19022	T19023	compound	reporter,constructs
R13520	T19023	T19019	pobj	constructs,with
R13521	T19024	T19023	punct	(,constructs
R13522	T19025	T19026	nummod	500,ng
R13523	T19026	T19023	appos	ng,constructs
R13524	T19027	T19023	punct	),constructs
R13525	T19028	T19018	prep	along,transfected
R13526	T19029	T19028	prep	with,along
R13527	T19030	T19032	preconj	either,vector
R13528	T19031	T19032	amod	empty,vector
R13529	T19032	T19029	pobj	vector,with
R13530	T19033	T19032	cc	or,vector
R13531	T19034	T19036	amod	Sox6,vector
R13532	T19035	T19036	compound	overexpression,vector
R13533	T19036	T19032	conj	vector,vector
R13534	T19037	T19036	punct	(,vector
R13535	T19038	T19039	nummod	1000,ng
R13536	T19039	T19036	appos	ng,vector
R13537	T19040	T19036	punct	),vector
R13538	T19041	T19018	punct	.,transfected
R13539	T19042	T19049	prep	In,used
R13540	T19043	T19042	pobj	assays,In
R13541	T19044	T19043	prep	of,assays
R13542	T19045	T19046	compound	dosage,effect
R13543	T19046	T19044	pobj	effect,of
R13544	T19047	T19049	punct	",",used
R13545	T19048	T19049	nsubj	we,used
R13546	T19049	T19049	ROOT	used,used
R13547	T19050	T19051	nummod	200,ng
R13548	T19051	T19049	dobj	ng,used
R13549	T19052	T19051	punct	",",ng
R13550	T19053	T19054	nummod	500,ng
R13551	T19054	T19051	appos	ng,ng
R13552	T19055	T19054	punct	",",ng
R13553	T19056	T19054	cc	and,ng
R13554	T19057	T19058	nummod	1000,ng
R13555	T19058	T19054	conj	ng,ng
R13556	T19059	T19051	punct	),ng
R13557	T19060	T19049	punct	.,used
R13558	T19061	T19062	amod	pRL-CMV,15ng
R13559	T19062	T19067	nsubjpass	15ng,used
R13560	T19063	T19064	punct	(,Promega
R13561	T19064	T19062	appos	Promega,15ng
R13562	T19065	T19064	punct	),Promega
R13563	T19066	T19067	auxpass	was,used
R13564	T19067	T19067	ROOT	used,used
R13565	T19068	T19067	prep	as,used
R13566	T19069	T19070	det	a,control
R13567	T19070	T19068	pobj	control,as
R13568	T19071	T19070	prep	for,control
R13569	T19072	T19073	compound	transfection,efficiency
R13570	T19073	T19071	pobj	efficiency,for
R13571	T19074	T19067	punct	.,used
R13673	T19267	T19268	compound	Nuclear,protein
R13674	T19268	T19269	nsubj	protein,extract
R13675	T19269	T19269	ROOT	extract,extract
R13676	T19270	T19269	cc	and,extract
R13677	T19271	T19269	prep	in,extract
R13678	T19272	T19273	amod	vitro,translation
R13679	T19273	T19271	pobj	translation,in
R13680	T19274	T19273	prep	of,translation
R13681	T19275	T19274	pobj	Sox6,of
R13682	T19276	T19269	punct	.,extract
R13683	T19277	T19278	compound	Nuclear,extracts
R13684	T19278	T19280	nsubjpass	extracts,prepared
R13685	T19279	T19280	auxpass	were,prepared
R13686	T19280	T19280	ROOT	prepared,prepared
R13687	T19281	T19280	prep	from,prepared
R13688	T19282	T19283	compound	MEL,cells
R13689	T19283	T19281	pobj	cells,from
R13690	T19284	T19283	punct	(,cells
R13691	T19285	T19286	nummod	2,×
R13692	T19286	T19287	nummod	×,107
R13693	T19287	T19283	appos	107,cells
R13694	T19288	T19283	punct	),cells
R13695	T19289	T19280	advcl	using,prepared
R13696	T19290	T19291	det	a,kit
R13697	T19291	T19289	dobj	kit,using
R13698	T19292	T19291	punct	(,kit
R13699	T19293	T19294	amod	Active,Motif
R13700	T19294	T19291	appos	Motif,kit
R13701	T19295	T19294	punct	",",Motif
R13702	T19296	T19294	conj	Carlsbad,Motif
R13703	T19297	T19296	punct	",",Carlsbad
R13704	T19298	T19296	conj	California,Carlsbad
R13705	T19299	T19298	punct	",",California
R13706	T19300	T19301	compound	United,States
R13707	T19301	T19298	conj	States,California
R13708	T19302	T19296	punct	),Carlsbad
R13709	T19303	T19280	punct	.,prepared
R13710	T19304	T19305	det	The,Sox6
R13711	T19305	T19312	nsubjpass	Sox6,tagged
R13712	T19306	T19312	prep	in,tagged
R13713	T19307	T19309	amod	vitro,expression
R13714	T19308	T19309	compound	translation,expression
R13715	T19309	T19310	compound	expression,vector
R13716	T19310	T19306	pobj	vector,in
R13717	T19311	T19312	punct	",",tagged
R13718	T19312	T19312	ROOT	tagged,tagged
R13719	T19313	T19312	prep	with,tagged
R13720	T19314	T19313	pobj	c-Myc,with
R13721	T19315	T19314	cc	and,c-Myc
R13722	T19316	T19314	conj	HA,c-Myc
R13723	T19317	T19319	punct	",",described
R13724	T19318	T19319	auxpass	was,described
R13725	T19319	T19312	conj	described,tagged
R13726	T19320	T19319	prep	before,described
R13727	T19321	T19323	nmod	[,]
R13728	T19322	T19321	nummod	15,[
R13729	T19323	T19320	pobj	],before
R13730	T19324	T19319	punct	.,described
R13731	T19325	T19326	det	The,translation
R13732	T19326	T19328	nsubjpass	translation,performed
R13733	T19327	T19328	auxpass	was,performed
R13734	T19328	T19328	ROOT	performed,performed
R13735	T19329	T19328	prep	in,performed
R13736	T19330	T19332	det	a,lysate
R13737	T19331	T19332	amod	reticulocyte,lysate
R13738	T19332	T19329	pobj	lysate,in
R13739	T19333	T19332	acl	based,lysate
R13740	T19334	T19333	prep	in,based
R13741	T19335	T19337	amod	vitro,system
R13742	T19336	T19337	amod	translational,system
R13743	T19337	T19334	pobj	system,in
R13744	T19338	T19337	punct	(,system
R13745	T19339	T19344	nmod	TNT,Systems
R13746	T19340	T19339	nummod	®,TNT
R13747	T19341	T19344	compound	Quick,Systems
R13748	T19342	T19344	compound	Coupled,Systems
R13749	T19343	T19344	compound	Transcription/Translation,Systems
R13750	T19344	T19337	appos	Systems,system
R13751	T19345	T19344	punct	",",Systems
R13752	T19346	T19344	appos	Promega,Systems
R13753	T19347	T19337	punct	),system
R13754	T19348	T19328	punct	.,performed
R13755	T19349	T19350	det	A,vector
R13756	T19350	T19358	nsubjpass	vector,translated
R13757	T19351	T19350	prep	without,vector
R13758	T19352	T19355	det	the,sequence
R13759	T19353	T19355	amod	Sox6,sequence
R13760	T19354	T19355	compound	coding,sequence
R13761	T19355	T19351	pobj	sequence,without
R13762	T19356	T19358	auxpass	was,translated
R13763	T19357	T19358	advmod	also,translated
R13764	T19358	T19358	ROOT	translated,translated
R13765	T19359	T19358	prep	as,translated
R13766	T19360	T19362	det	a,control
R13767	T19361	T19362	amod	negative,control
R13768	T19362	T19359	pobj	control,as
R13769	T19363	T19358	punct	.,translated
R13850	T19522	T19522	ROOT	Antibodies,Antibodies
R13851	T19523	T19522	punct	.,Antibodies
R13852	T19524	T19525	amod	Sox6,antibodies
R13853	T19525	T19530	nsubj	antibodies,were
R13854	T19526	T19525	acl	used,antibodies
R13855	T19527	T19526	prep	in,used
R13856	T19528	T19529	det	this,study
R13857	T19529	T19527	pobj	study,in
R13858	T19530	T19533	auxpass	were,provided
R13859	T19531	T19533	preconj	either,provided
R13860	T19532	T19533	advmod	kindly,provided
R13861	T19533	T19533	ROOT	provided,provided
R13862	T19534	T19533	agent	by,provided
R13863	T19535	T19537	compound	Dr.,Lalli
R13864	T19536	T19537	compound	Enzo,Lalli
R13865	T19537	T19534	pobj	Lalli,by
R13866	T19538	T19541	punct	(,Pasteur
R13867	T19539	T19541	compound	Université,Pasteur
R13868	T19540	T19541	compound	Louis,Pasteur
R13869	T19541	T19537	appos	Pasteur,Lalli
R13870	T19542	T19541	punct	",",Pasteur
R13871	T19543	T19550	nsubj	France,obtained
R13872	T19544	T19546	nmod	[,]
R13873	T19545	T19546	nummod	7,]
R13874	T19546	T19543	nmod	],France
R13875	T19547	T19546	punct	),]
R13876	T19548	T19543	cc	or,France
R13877	T19549	T19550	advmod	commercially,obtained
R13878	T19550	T19533	conj	obtained,provided
R13879	T19551	T19556	punct	(,X
R13880	T19552	T19553	compound	Catalog,No
R13881	T19553	T19556	nmod	No,X
R13882	T19554	T19556	punct	.,X
R13883	T19555	T19556	compound	sc-17332,X
R13884	T19556	T19550	dobj	X,obtained
R13885	T19557	T19556	punct	",",X
R13886	T19558	T19559	compound	Santa,Cruz
R13887	T19559	T19560	compound	Cruz,Biotechnology
R13888	T19560	T19556	conj	Biotechnology,X
R13889	T19561	T19560	punct	",",Biotechnology
R13890	T19562	T19563	compound	Santa,Cruz
R13891	T19563	T19560	conj	Cruz,Biotechnology
R13892	T19564	T19563	punct	",",Cruz
R13893	T19565	T19563	conj	California,Cruz
R13894	T19566	T19565	punct	",",California
R13895	T19567	T19568	compound	United,States
R13896	T19568	T19565	conj	States,California
R13897	T19569	T19550	punct	),obtained
R13898	T19570	T19533	punct	.,provided
R13899	T19571	T19573	det	All,antibodies
R13900	T19572	T19573	amod	Sox6,antibodies
R13901	T19573	T19574	nsubj	antibodies,generated
R13902	T19574	T19574	ROOT	generated,generated
R13903	T19575	T19576	amod	similar,results
R13904	T19576	T19574	dobj	results,generated
R13905	T19577	T19574	punct	.,generated
R13906	T19578	T19579	amod	c-Myc,antibody
R13907	T19579	T19581	nsubjpass	antibody,purchased
R13908	T19580	T19581	auxpass	was,purchased
R13909	T19581	T19581	ROOT	purchased,purchased
R13911	T19583	T19582	pobj	Invitrogen,from
R13912	T19584	T19581	punct	.,purchased
R13913	T19585	T19588	amod	Normal,antibody
R13914	T19586	T19588	compound	rabbit,antibody
R13915	T19587	T19588	compound	IgG,antibody
R13916	T19588	T19590	nsubjpass	antibody,obtained
R13917	T19589	T19590	auxpass	was,obtained
R13918	T19590	T19590	ROOT	obtained,obtained
R13919	T19591	T19590	prep	from,obtained
R13920	T19592	T19593	compound	Upstate,Biotechnology
R13921	T19593	T19591	pobj	Biotechnology,from
R13922	T19594	T19596	punct	(,Placid
R13923	T19595	T19596	compound	Lake,Placid
R13924	T19596	T19593	appos	Placid,Biotechnology
R13925	T19597	T19596	punct	",",Placid
R13926	T19598	T19599	compound	New,York
R13927	T19599	T19596	npadvmod	York,Placid
R13928	T19600	T19599	punct	",",York
R13929	T19601	T19602	compound	United,States
R13930	T19602	T19599	appos	States,York
R13931	T19603	T19596	punct	),Placid
R13932	T19604	T19590	punct	.,obtained
R14199	T19985	T19985	ROOT	EMSA,EMSA
R14200	T19986	T19985	punct	.,EMSA
R14201	T19987	T19989	amod	Single-stranded,oligonucleotides
R14202	T19988	T19989	amod	complementary,oligonucleotides
R14203	T19989	T19990	nsubj	oligonucleotides,were
R14204	T19990	T19990	ROOT	were,were
R14205	T19991	T19990	acomp	annealed,were
R14206	T19992	T19991	cc	and,annealed
R14207	T19993	T19991	conj	end-labeled,annealed
R14208	T19994	T19991	prep	with,annealed
R14209	T19995	T19998	compound	[,ATP
R14210	T19996	T19998	compound	γ-32P,ATP
R14211	T19997	T19998	compound	],ATP
R14212	T19998	T19994	pobj	ATP,with
R14213	T19999	T19994	prep	with,with
R14214	T20000	T20002	nummod	T4,kinase
R14215	T20001	T20002	compound	polynucleotide,kinase
R14216	T20002	T19999	pobj	kinase,with
R14217	T20003	T19990	punct	.,were
R14218	T20004	T20006	nsubjpass	EMSA,performed
R14219	T20005	T20006	auxpass	was,performed
R14220	T20006	T20006	ROOT	performed,performed
R14221	T20007	T20006	prep	with,performed
R14222	T20008	T20009	nummod	5,μg
R14223	T20009	T20007	pobj	μg,with
R14224	T20010	T20009	prep	of,μg
R14225	T20011	T20012	amod	nuclear,proteins
R14226	T20012	T20010	pobj	proteins,of
R14227	T20013	T20009	prep	from,μg
R14228	T20014	T20015	compound	MEL,cells
R14229	T20015	T20013	pobj	cells,from
R14230	T20016	T20015	cc	or,cells
R14231	T20017	T20018	nummod	3,μl
R14232	T20018	T20015	conj	μl,cells
R14233	T20019	T20018	prep	of,μl
R14234	T20020	T20018	prep	in,μl
R14235	T20021	T20022	amod	vitro-translated,Sox6
R14236	T20022	T20020	pobj	Sox6,in
R14237	T20023	T20009	prep	along,μg
R14238	T20024	T20023	prep	with,along
R14239	T20025	T20027	det	the,lysate
R14240	T20026	T20027	compound	reticulocyte,lysate
R14241	T20027	T20024	pobj	lysate,with
R14242	T20028	T20027	prep	in,lysate
R14243	T20029	T20030	amod	binding,buffer
R14244	T20030	T20028	pobj	buffer,in
R14245	T20031	T20009	punct	:,μg
R14246	T20032	T20034	nummod	100,NaCl
R14247	T20033	T20034	compound	mM,NaCl
R14248	T20034	T20009	appos	NaCl,μg
R14249	T20035	T20034	punct	",",NaCl
R14250	T20036	T20037	nummod	10,%
R14251	T20037	T20038	compound	%,glycerol
R14252	T20038	T20034	appos	glycerol,NaCl
R14253	T20039	T20038	punct	",",glycerol
R14254	T20040	T20041	nummod	200,ng
R14255	T20041	T20050	npadvmod	ng,poly
R14256	T20042	T20044	nmod	/,BSA
R14257	T20043	T20044	compound	μl,BSA
R14258	T20044	T20050	nmod	BSA,poly
R14259	T20045	T20050	punct	",",poly
R14260	T20046	T20047	nummod	50,ng
R14261	T20047	T20050	compound	ng,poly
R14262	T20048	T20050	compound	/,poly
R14263	T20049	T20050	compound	μl,poly
R14264	T20050	T20009	appos	poly,μg
R14265	T20051	T20050	punct	(,poly
R14266	T20052	T20050	appos	dI-dC,poly
R14267	T20053	T20052	punct	),dI-dC
R14268	T20054	T20052	cc	or,dI-dC
R14269	T20055	T20055	ROOT	poly,poly
R14270	T20056	T20057	punct	(,dG-dC
R14271	T20057	T20055	appos	dG-dC,poly
R14272	T20058	T20057	punct	),dG-dC
R14273	T20059	T20055	punct	",",poly
R14274	T20060	T20062	nummod	10,HEPES
R14275	T20061	T20062	compound	mM,HEPES
R14276	T20062	T20055	appos	HEPES,poly
R14277	T20063	T20064	punct	(,pH
R14278	T20064	T20062	parataxis	pH,HEPES
R14279	T20065	T20064	nummod	7,pH
R14280	T20066	T20064	punct	),pH
R14281	T20067	T20064	punct	",",pH
R14282	T20068	T20070	nummod	0.1,EDTA
R14283	T20069	T20070	compound	mM,EDTA
R14284	T20070	T20064	appos	EDTA,pH
R14285	T20071	T20070	punct	",",EDTA
R14286	T20072	T20074	nummod	0.25,DTT
R14287	T20073	T20074	compound	mM,DTT
R14288	T20074	T20070	appos	DTT,EDTA
R14289	T20075	T20074	punct	",",DTT
R14290	T20076	T20078	nummod	0.6,MgCl2
R14291	T20077	T20078	compound	mM,MgCl2
R14292	T20078	T20074	appos	MgCl2,DTT
R14293	T20079	T20006	punct	.,performed
R14294	T20080	T20103	prep	For,added
R14295	T20081	T20080	pobj	competition,For
R14296	T20082	T20081	cc	or,competition
R14297	T20083	T20084	compound	supershift,assays
R14298	T20084	T20081	conj	assays,competition
R14299	T20085	T20084	punct	",",assays
R14300	T20086	T20090	det	the,competitor
R14301	T20087	T20090	amod	indicated,competitor
R14302	T20088	T20090	amod	unlabelled,competitor
R14303	T20089	T20090	compound	oligonucleotide,competitor
R14304	T20090	T20084	conj	competitor,assays
R14305	T20091	T20090	punct	(,competitor
R14306	T20092	T20094	amod	200-fold,excess
R14307	T20093	T20094	compound	molar,excess
R14308	T20094	T20090	appos	excess,competitor
R14309	T20095	T20090	punct	),competitor
R14310	T20096	T20090	cc	or,competitor
R14311	T20097	T20090	conj	antibody,competitor
R14312	T20098	T20097	punct	(,antibody
R14313	T20099	T20100	nummod	3,μl
R14314	T20100	T20097	appos	μl,antibody
R14315	T20101	T20097	punct	),antibody
R14316	T20102	T20103	auxpass	was,added
R14317	T20103	T20103	ROOT	added,added
R14318	T20104	T20107	quantmod	30,60
R14319	T20105	T20107	quantmod	min,60
R14320	T20106	T20107	quantmod	to,60
R14321	T20107	T20103	dobj	60,added
R14322	T20108	T20107	quantmod	min,60
R14323	T20109	T20103	advmod	prior,added
R14324	T20110	T20103	prep	to,added
R14325	T20111	T20110	pobj	addition,to
R14326	T20112	T20111	prep	of,addition
R14327	T20113	T20114	amod	radiolabeled,probe
R14328	T20114	T20112	pobj	probe,of
R14329	T20115	T20103	punct	.,added
R14330	T20116	T20126	prep	Following,incubated
R14331	T20117	T20116	pobj	addition,Following
R14332	T20118	T20117	prep	of,addition
R14333	T20119	T20121	det	the,probe
R14334	T20120	T20121	compound	radiolabeled,probe
R14335	T20121	T20118	pobj	probe,of
R14336	T20122	T20126	punct	",",incubated
R14337	T20123	T20124	det	the,samples
R14338	T20124	T20126	nsubjpass	samples,incubated
R14339	T20125	T20126	auxpass	were,incubated
R14340	T20126	T20126	ROOT	incubated,incubated
R14341	T20127	T20126	prep	for,incubated
R14342	T20128	T20129	nummod	30,min
R14343	T20129	T20127	pobj	min,for
R14344	T20130	T20129	cc	or,min
R14345	T20131	T20132	nummod	60,min
R14346	T20132	T20129	conj	min,min
R14347	T20133	T20132	prep	at,min
R14348	T20134	T20135	compound	room,temperature
R14349	T20135	T20133	pobj	temperature,at
R14350	T20136	T20132	cc	and,min
R14351	T20137	T20129	acl	loaded,min
R14352	T20138	T20137	prep	on,loaded
R14353	T20139	T20141	det	a,%
R14354	T20140	T20141	nummod	4,%
R14355	T20141	T20138	pobj	%,on
R14356	T20142	T20141	cc	or,%
R14357	T20143	T20144	nummod	6,%
R14358	T20144	T20141	conj	%,%
R14359	T20145	T20146	punct	(,w/v
R14360	T20146	T20144	appos	w/v,%
R14361	T20147	T20126	punct	),incubated
R14362	T20148	T20149	compound	polyacrylamide,gel
R14363	T20149	T20149	ROOT	gel,gel
R14364	T20150	T20149	punct	.,gel
R14365	T20151	T20153	nsubjpass	Electrophoresis,performed
R14366	T20152	T20153	auxpass	was,performed
R14367	T20153	T20153	ROOT	performed,performed
R14368	T20154	T20153	prep	at,performed
R14369	T20155	T20158	det	a,mAmp
R14370	T20156	T20158	amod	constant,mAmp
R14371	T20157	T20158	nummod	19,mAmp
R14372	T20158	T20154	pobj	mAmp,at
R14373	T20159	T20153	prep	for,performed
R14374	T20160	T20159	pobj	4,for
R14375	T20161	T20162	nummod	8,h
R14376	T20162	T20171	nsubjpass	h,dried
R14377	T20163	T20162	prep	at,h
R14378	T20164	T20165	compound	room,temperature
R14379	T20165	T20163	pobj	temperature,at
R14380	T20166	T20162	punct	",",h
R14381	T20167	T20162	cc	and,h
R14382	T20168	T20169	det	the,gels
R14383	T20169	T20162	conj	gels,h
R14384	T20170	T20171	auxpass	were,dried
R14385	T20171	T20153	conj	dried,performed
R14386	T20172	T20171	advmod	prior,dried
R14387	T20173	T20172	prep	to,prior
R14388	T20174	T20173	pobj	autoradiography,to
R14389	T20175	T20171	punct	.,dried
R14390	T20176	T20177	nsubj	Antibodies,used
R14391	T20177	T20177	ROOT	used,used
R14392	T20178	T20177	prep	for,used
R14393	T20179	T20180	compound	supershift,analyses
R14394	T20180	T20178	pobj	analyses,for
R14395	T20181	T20177	conj	included,used
R14396	T20182	T20188	nmod	c-Myc,antibodies
R14397	T20183	T20182	punct	",",c-Myc
R14398	T20184	T20182	conj	HA,c-Myc
R14399	T20185	T20184	punct	",",HA
R14400	T20186	T20184	cc	and,HA
R14401	T20187	T20184	conj	Sox6,HA
R14402	T20188	T20181	dobj	antibodies,included
R14403	T20189	T20188	punct	(,antibodies
R14404	T20190	T20188	acl	described,antibodies
R14405	T20191	T20190	prep	as,described
R14406	T20192	T20191	pcomp	above,as
R14407	T20193	T20188	punct	),antibodies
R14408	T20194	T20177	punct	.,used
R14409	T20195	T20197	det	The,sequences
R14410	T20196	T20197	compound	DNA,sequences
R14411	T20197	T20201	nsubj	sequences,are
R14412	T20198	T20197	prep	of,sequences
R14413	T20199	T20200	det	the,oligonucleotides
R14414	T20200	T20198	pobj	oligonucleotides,of
R14415	T20201	T20201	ROOT	are,are
R14416	T20202	T20203	mark	as,follows
R14417	T20203	T20201	advcl	follows,are
R14418	T20204	T20209	punct	(,listed
R14419	T20205	T20206	advmod	only,forward
R14420	T20206	T20209	advmod	forward,listed
R14421	T20207	T20209	nsubjpass	oligos,listed
R14422	T20208	T20209	auxpass	are,listed
R14423	T20209	T20201	advcl	listed,are
R14424	T20210	T20209	punct	),listed
R14425	T20211	T20237	punct	:,′
R14426	T20212	T20237	prep	For,′
R14427	T20213	T20216	det	the,probe
R14428	T20214	T20216	amod	36-bp,probe
R14429	T20215	T20216	compound	WT,probe
R14430	T20216	T20212	pobj	probe,For
R14431	T20217	T20237	punct	:,′
R14432	T20218	T20221	nummod	5,′
R14433	T20219	T20221	nummod	′,′
R14434	T20220	T20221	compound	AATGCAGAACAAAGGGTCAGAACATTGTCTGCGAAG3,′
R14435	T20221	T20237	npadvmod	′,′
R14436	T20222	T20223	punct	(,MHB1556
R14437	T20223	T20221	appos	MHB1556,′
R14438	T20224	T20223	punct	),MHB1556
R14439	T20225	T20237	punct	;,′
R14440	T20226	T20237	prep	for,′
R14441	T20227	T20228	amod	mutant,probe
R14442	T20228	T20226	pobj	probe,for
R14443	T20229	T20228	nummod	1,probe
R14444	T20230	T20228	punct	(,probe
R14445	T20231	T20228	appos	M1,probe
R14446	T20232	T20228	punct	),probe
R14447	T20233	T20237	punct	:,′
R14448	T20234	T20237	nummod	5,′
R14449	T20235	T20237	nummod	′,′
R14450	T20236	T20237	compound	AACAAAGGGTCAGAACATTGTCTGCGAAG3,′
R14451	T20237	T20237	ROOT	′,′
R14452	T20238	T20239	punct	(,MHB1644
R14453	T20239	T20237	appos	MHB1644,′
R14454	T20240	T20239	punct	),MHB1644
R14455	T20241	T20253	punct	;,′
R14456	T20242	T20253	prep	for,′
R14457	T20243	T20244	amod	mutant,probe
R14458	T20244	T20242	pobj	probe,for
R14459	T20245	T20244	nummod	2,probe
R14460	T20246	T20244	punct	(,probe
R14461	T20247	T20244	appos	M2,probe
R14462	T20248	T20244	punct	),probe
R14463	T20249	T20253	punct	:,′
R14464	T20250	T20251	nummod	5,′
R14465	T20251	T20253	compound	′,′
R14466	T20252	T20253	compound	AATGCAGAACAAAGGGTCAGAtgagTGTCTGCGAAG3,′
R14467	T20253	T20237	conj	′,′
R14468	T20254	T20253	punct	(,′
R14469	T20255	T20253	appos	MHB1648,′
R14470	T20256	T20253	punct	),′
R14471	T20257	T20253	punct	;,′
R14472	T20258	T20258	ROOT	for,for
R14473	T20259	T20260	amod	mutant,probe
R14474	T20260	T20258	pobj	probe,for
R14475	T20261	T20260	nummod	3,probe
R14476	T20262	T20260	punct	(,probe
R14477	T20263	T20260	appos	M3,probe
R14478	T20264	T20260	punct	),probe
R14479	T20265	T20258	punct	:,for
R14480	T20266	T20267	nummod	5,′
R14481	T20267	T20269	compound	′,′
R14482	T20268	T20269	compound	AATGCAGtgccAAGGGTCAGAACATTGTCTGCGAAG3,′
R14483	T20269	T20269	ROOT	′,′
R14484	T20270	T20269	punct	(,′
R14485	T20271	T20269	appos	MHB1650,′
R14486	T20272	T20269	punct	),′
R14487	T20273	T20237	punct	.,′
R14776	T20678	T20679	compound	ChIP,assay
R14777	T20679	T20679	ROOT	assay,assay
R14778	T20680	T20679	punct	.,assay
R14779	T20681	T20682	mark	As,described
R14780	T20682	T20711	advcl	described,treated
R14781	T20683	T20682	agent	by,described
R14782	T20684	T20685	compound	Nouzova,[
R14783	T20685	T20687	nmod	[,]
R14784	T20686	T20687	nummod	53,]
R14785	T20687	T20683	pobj	],by
R14786	T20688	T20682	punct	",",described
R14787	T20689	T20682	prep	in,described
R14788	T20690	T20689	pobj	brief,in
R14789	T20691	T20711	punct	:,treated
R14790	T20692	T20711	nsubjpass	Cells,treated
R14791	T20693	T20692	prep	from,Cells
R14792	T20694	T20695	compound	MEL,cells
R14793	T20695	T20693	pobj	cells,from
R14794	T20696	T20695	punct	(,cells
R14795	T20697	T20699	nummod	4,107
R14796	T20698	T20699	nummod	×,107
R14797	T20699	T20695	appos	107,cells
R14798	T20700	T20699	punct	),107
R14799	T20701	T20699	cc	or,107
R14800	T20702	T20704	amod	fetal,cells
R14801	T20703	T20704	compound	liver,cells
R14802	T20704	T20711	nsubjpass	cells,treated
R14803	T20705	T20704	prep	from,cells
R14804	T20706	T20709	nummod	three,mice
R14805	T20707	T20709	amod	15.5-dpc,mice
R14806	T20708	T20709	compound	WT,mice
R14807	T20709	T20705	pobj	mice,from
R14808	T20710	T20711	auxpass	were,treated
R14809	T20711	T20711	ROOT	treated,treated
R14810	T20712	T20711	prep	with,treated
R14811	T20713	T20714	nummod	1,%
R14812	T20714	T20715	compound	%,formaldehyde
R14813	T20715	T20712	pobj	formaldehyde,with
R14814	T20716	T20711	prep	for,treated
R14815	T20717	T20718	nummod	10,min
R14816	T20718	T20716	pobj	min,for
R14817	T20719	T20711	prep	at,treated
R14818	T20720	T20721	nummod	37,°
R14819	T20721	T20722	nummod	°,C
R14820	T20722	T20719	pobj	C,at
R14821	T20723	T20711	punct	",",treated
R14822	T20724	T20711	conj	rinsed,treated
R14823	T20725	T20724	prep	in,rinsed
R14824	T20726	T20729	amod	ice-cold,Hanks
R14825	T20727	T20728	nummod	1,×
R14826	T20728	T20729	compound	×,Hanks
R14827	T20729	T20733	poss	Hanks,solution
R14828	T20730	T20729	case	',Hanks
R14829	T20731	T20733	amod	balanced,solution
R14830	T20732	T20733	compound	salt,solution
R14831	T20733	T20725	pobj	solution,in
R14832	T20734	T20733	prep	with,solution
R14833	T20735	T20736	nummod	0.1,%
R14834	T20736	T20737	compound	%,EDTA
R14835	T20737	T20738	nsubj	EDTA,containing
R14836	T20738	T20734	pcomp	containing,with
R14837	T20739	T20740	compound	protease,inhibitors
R14838	T20740	T20738	dobj	inhibitors,containing
R14839	T20741	T20740	punct	",",inhibitors
R14840	T20742	T20740	acl	collected,inhibitors
R14841	T20743	T20742	agent	by,collected
R14842	T20744	T20743	pobj	centrifugation,by
R14843	T20745	T20742	prep	at,collected
R14844	T20746	T20747	nummod	4,°
R14845	T20747	T20748	nummod	°,C
R14846	T20748	T20745	pobj	C,at
R14847	T20749	T20748	punct	",",C
R14848	T20750	T20724	conj	resuspended,rinsed
R14849	T20751	T20750	prep	in,resuspended
R14850	T20752	T20755	det	a,buffer
R14851	T20753	T20755	compound	SDS,buffer
R14852	T20754	T20755	compound	lysis,buffer
R14853	T20755	T20751	pobj	buffer,in
R14854	T20756	T20755	acl	containing,buffer
R14855	T20757	T20758	compound	protease,inhibitors
R14856	T20758	T20756	dobj	inhibitors,containing
R14857	T20759	T20750	punct	",",resuspended
R14858	T20760	T20750	cc	and,resuspended
R14859	T20761	T20750	conj	incubated,resuspended
R14860	T20762	T20761	prep	on,incubated
R14861	T20763	T20762	pobj	ice,on
R14862	T20764	T20761	prep	for,incubated
R14863	T20765	T20766	nummod	10,min
R14864	T20766	T20764	pobj	min,for
R14865	T20767	T20711	punct	.,treated
R14866	T20768	T20769	amod	DNA-protein,complexes
R14867	T20769	T20771	nsubjpass	complexes,sonicated
R14868	T20770	T20771	auxpass	were,sonicated
R14869	T20771	T20771	ROOT	sonicated,sonicated
R14870	T20772	T20771	prep	to,sonicated
R14871	T20773	T20776	nummod	200,bp
R14872	T20774	T20773	cc	and,200
R14873	T20775	T20773	conj	600,200
R14874	T20776	T20772	pobj	bp,to
R14875	T20777	T20771	punct	.,sonicated
R14876	T20778	T20783	nsubjpass	One-tenth,set
R14877	T20779	T20778	prep	of,One-tenth
R14878	T20780	T20781	det	the,sample
R14879	T20781	T20779	pobj	sample,of
R14880	T20782	T20783	auxpass	was,set
R14881	T20783	T20783	ROOT	set,set
R14882	T20784	T20783	advmod	aside,set
R14883	T20785	T20783	prep	for,set
R14884	T20786	T20787	compound	input,control
R14885	T20787	T20785	pobj	control,for
R14886	T20788	T20783	punct	",",set
R14887	T20789	T20783	cc	and,set
R14888	T20790	T20792	det	the,sample
R14889	T20791	T20792	amod	remaining,sample
R14890	T20792	T20794	nsubjpass	sample,precleared
R14891	T20793	T20794	auxpass	was,precleared
R14892	T20794	T20783	conj	precleared,set
R14893	T20795	T20794	prep	with,precleared
R14894	T20796	T20797	compound	protein,A-Sepharose
R14895	T20797	T20795	pobj	A-Sepharose,with
R14896	T20798	T20800	punct	(,Biosciences
R14897	T20799	T20800	compound	Amersham,Biosciences
R14898	T20800	T20797	appos	Biosciences,A-Sepharose
R14899	T20801	T20800	punct	",",Biosciences
R14900	T20802	T20800	npadvmod	Piscataway,Biosciences
R14901	T20803	T20802	punct	",",Piscataway
R14902	T20804	T20805	compound	New,Jersey
R14903	T20805	T20802	conj	Jersey,Piscataway
R14904	T20806	T20805	punct	",",Jersey
R14905	T20807	T20808	compound	United,States
R14906	T20808	T20802	appos	States,Piscataway
R14907	T20809	T20802	punct	),Piscataway
R14908	T20810	T20794	punct	.,precleared
R14909	T20811	T20817	prep	Following,split
R14910	T20812	T20811	pobj	preclearing,Following
R14911	T20813	T20817	punct	",",split
R14912	T20814	T20815	det	the,samples
R14913	T20815	T20817	nsubjpass	samples,split
R14914	T20816	T20817	auxpass	were,split
R14915	T20817	T20817	ROOT	split,split
R14916	T20818	T20817	prep	into,split
R14917	T20819	T20818	pobj	thirds,into
R14918	T20820	T20817	punct	:,split
R14919	T20821	T20822	nummod	one,sample
R14920	T20822	T20829	nsubjpass	sample,treated
R14921	T20823	T20822	acl	treated,sample
R14922	T20824	T20823	prep	with,treated
R14923	T20825	T20824	pobj	anti-Sox6,with
R14924	T20826	T20825	punct	",",anti-Sox6
R14925	T20827	T20828	det	a,second
R14926	T20828	T20829	amod	second,treated
R14927	T20829	T20817	conj	treated,split
R14928	T20830	T20829	prep	with,treated
R14929	T20831	T20832	amod	normal,rabbit
R14930	T20832	T20833	compound	rabbit,IgG
R14931	T20833	T20830	pobj	IgG,with
R14932	T20834	T20829	punct	",",treated
R14933	T20835	T20829	cc	and,treated
R14934	T20836	T20838	det	the,sample
R14935	T20837	T20838	amod	third,sample
R14936	T20838	T20829	conj	sample,treated
R14937	T20839	T20838	prep	without,sample
R14938	T20840	T20839	pobj	Ab,without
R14939	T20841	T20829	punct	.,treated
R14940	T20842	T20844	det	The,two
R14941	T20843	T20844	amod	last,two
R14942	T20844	T20846	nsubjpass	two,used
R14943	T20845	T20846	auxpass	were,used
R14944	T20846	T20846	ROOT	used,used
R14945	T20847	T20846	prep	as,used
R14946	T20848	T20849	amod	negative,controls
R14947	T20849	T20847	pobj	controls,as
R14948	T20850	T20846	punct	.,used
R14949	T20851	T20853	det	The,complexes
R14950	T20852	T20853	amod	chromatin-antibody,complexes
R14951	T20853	T20855	nsubjpass	complexes,eluted
R14952	T20854	T20855	auxpass	were,eluted
R14953	T20855	T20855	ROOT	eluted,eluted
R14954	T20856	T20855	punct	",",eluted
R14955	T20857	T20855	cc	and,eluted
R14956	T20858	T20860	det	the,protein
R14957	T20859	T20860	compound	DNA,protein
R14958	T20860	T20861	compound	protein,cross-links
R14959	T20861	T20863	nsubjpass	cross-links,reversed
R14960	T20862	T20863	auxpass	were,reversed
R14961	T20863	T20855	conj	reversed,eluted
R14962	T20864	T20863	prep	with,reversed
R14963	T20865	T20867	nummod	5,NaCl
R14964	T20866	T20867	compound	M,NaCl
R14965	T20867	T20864	pobj	NaCl,with
R14966	T20868	T20863	prep	at,reversed
R14967	T20869	T20871	nummod	65,C
R14968	T20870	T20871	nummod	°,C
R14969	T20871	T20868	pobj	C,at
R14970	T20872	T20863	prep	for,reversed
R14971	T20873	T20874	nummod	4,h.
R14972	T20874	T20876	compound	h.,DNA
R14973	T20875	T20876	compound	Input,DNA
R14974	T20876	T20872	pobj	DNA,for
R14975	T20877	T20876	cc	or,DNA
R14976	T20878	T20879	amod	immunoprecipitated,DNA
R14977	T20879	T20876	conj	DNA,DNA
R14978	T20880	T20881	auxpass	was,used
R14979	T20881	T20863	conj	used,reversed
R14980	T20882	T20881	prep	as,used
R14981	T20883	T20884	det	a,template
R14982	T20884	T20882	pobj	template,as
R14983	T20885	T20884	prep	in,template
R14984	T20886	T20888	det	the,reaction
R14985	T20887	T20888	compound	PCR,reaction
R14986	T20888	T20885	pobj	reaction,in
R14987	T20889	T20863	punct	.,reversed
R14988	T20890	T20891	compound	PCR,amplification
R14989	T20891	T20897	nsubjpass	amplification,performed
R14990	T20892	T20891	prep	of,amplification
R14991	T20893	T20895	det	the,promoter
R14992	T20894	T20895	amod	ɛy,promoter
R14993	T20895	T20892	pobj	promoter,of
R14994	T20896	T20897	auxpass	was,performed
R14995	T20897	T20897	ROOT	performed,performed
R14996	T20898	T20897	cc	and,performed
R14997	T20899	T20897	conj	yielded,performed
R14998	T20900	T20902	det	a,amplicon
R14999	T20901	T20902	amod	172-bp,amplicon
R15000	T20902	T20899	dobj	amplicon,yielded
R15001	T20903	T20902	punct	",",amplicon
R15002	T20904	T20902	amod	corresponding,amplicon
R15003	T20905	T20907	aux	to,−
R15004	T20906	T20905	pobj	nucleotides,to
R15005	T20907	T20904	xcomp	−,corresponding
R15006	T20908	T20910	quantmod	31,+140
R15007	T20909	T20910	quantmod	to,+140
R15008	T20910	T20907	dobj	+140,−
R15009	T20911	T20910	prep	of,+140
R15010	T20912	T20914	det	the,promoter
R15011	T20913	T20914	amod	ɛy,promoter
R15012	T20914	T20911	pobj	promoter,of
R15013	T20915	T20916	punct	(,primers
R15014	T20916	T20914	appos	primers,promoter
R15015	T20917	T20922	nmod	MHB1688,′
R15016	T20918	T20917	punct	",",MHB1688
R15017	T20919	T20920	nummod	5,′
R15018	T20920	T20922	nmod	′,′
R15019	T20921	T20922	nummod	CGAAGAATAAAAGGCCACCA3,′
R15020	T20922	T20916	dobj	′,primers
R15021	T20923	T20916	punct	;,primers
R15022	T20924	T20916	cc	and,primers
R15023	T20925	T20916	conj	MHB1689,primers
R15024	T20926	T20925	punct	",",MHB1689
R15025	T20927	T20928	nummod	5,′
R15026	T20928	T20930	compound	′,′
R15027	T20929	T20930	compound	GCTTCACCACCAACCTCTTC3,′
R15028	T20930	T20925	conj	′,MHB1689
R15029	T20931	T20930	punct	),′
R15030	T20932	T20897	punct	.,performed
R15031	T20933	T20935	nsubjpass	PCR,performed
R15032	T20934	T20935	auxpass	was,performed
R15033	T20935	T20935	ROOT	performed,performed
R15034	T20936	T20935	prep	under,performed
R15035	T20937	T20939	det	the,conditions
R15036	T20938	T20939	amod	following,conditions
R15037	T20939	T20936	pobj	conditions,under
R15038	T20940	T20939	punct	:,conditions
R15039	T20941	T20942	nummod	95,°
R15040	T20942	T20943	compound	°,C
R15041	T20943	T20939	appos	C,conditions
R15042	T20944	T20943	prep	for,C
R15043	T20945	T20946	nummod	15,min
R15044	T20946	T20944	pobj	min,for
R15045	T20947	T20939	acl	followed,conditions
R15046	T20948	T20947	agent	by,followed
R15047	T20949	T20950	nummod	30,cycles
R15048	T20950	T20948	pobj	cycles,by
R15049	T20951	T20950	prep	at,cycles
R15050	T20952	T20951	pobj	95,at
R15051	T20953	T20954	nummod	°,C
R15052	T20954	T20950	conj	C,cycles
R15053	T20955	T20947	prep	for,followed
R15054	T20956	T20955	pobj	30,for
R15055	T20957	T20939	appos	s,conditions
R15056	T20958	T20935	punct	",",performed
R15057	T20959	T20960	nummod	60,°
R15058	T20960	T20961	compound	°,C
R15059	T20961	T20935	npadvmod	C,performed
R15060	T20962	T20935	prep	for,performed
R15061	T20963	T20962	pobj	30,for
R15062	T20964	T20935	dep	s,performed
R15063	T20965	T20935	punct	",",performed
R15064	T20966	T20935	cc	and,performed
R15065	T20967	T20969	nummod	72,C
R15066	T20968	T20969	compound	°,C
R15067	T20969	T20935	conj	C,performed
R15068	T20970	T20969	prep	for,C
R15069	T20971	T20970	pobj	45,for
R15070	T20972	T20969	appos	s,C
R15071	T20973	T20969	punct	",",C
R15072	T20974	T20935	advcl	ending,performed
R15073	T20975	T20974	prep	with,ending
R15074	T20976	T20978	det	a,extension
R15075	T20977	T20978	amod	final,extension
R15076	T20978	T20975	pobj	extension,with
R15077	T20979	T20978	prep	at,extension
R15078	T20980	T20981	nummod	72,°
R15079	T20981	T20979	pobj	°,at
R15080	T20982	T20978	npadvmod	C,extension
R15081	T20983	T20978	prep	for,extension
R15082	T20984	T20985	nummod	5,min
R15083	T20985	T20983	pobj	min,for
R15084	T20986	T20935	punct	.,performed
R12562	T17961	T17961	ROOT	In,In
R12563	T17962	T17963	compound	situ,hybridization
R12564	T17963	T17961	pobj	hybridization,In
R12565	T17964	T17963	punct	.,hybridization
R12566	T17965	T17966	compound	Antisense,probes
R12567	T17966	T17968	nsubjpass	probes,designed
R12568	T17967	T17968	auxpass	were,designed
R12569	T17968	T17968	ROOT	designed,designed
R12570	T17969	T17970	aux	to,murine
R12571	T17970	T17968	xcomp	murine,designed
R12572	T17971	T17973	amod	ɛy,nucleotides
R12573	T17972	T17973	compound	globin,nucleotides
R12574	T17973	T17970	dobj	nucleotides,murine
R12575	T17974	T17970	npadvmod	509,murine
R12576	T17975	T17970	meta	584,murine
R12577	T17976	T17968	punct	;,designed
R12578	T17977	T17968	conj	βmaj,designed
R12579	T17978	T17979	compound	globin,nucleotides
R12580	T17979	T17977	dobj	nucleotides,βmaj
R12581	T17980	T17979	nummod	458,nucleotides
R11814	T16898	T16899	amod	truncated,version
R11815	T16899	T16904	nsubj	version,construct
R11816	T16900	T16899	prep	of,version
R11817	T16901	T16903	det	the,overexpression
R11818	T16902	T16903	amod	Sox6,overexpression
R11819	T16903	T16900	pobj	overexpression,of
R11820	T16904	T16904	ROOT	construct,construct
R11821	T16905	T16908	punct	(,)
R11822	T16906	T16908	nmod	Sox6-ΔHMG-pcDNA3,)
R11823	T16907	T16906	nummod	.1,Sox6-ΔHMG-pcDNA3
R11824	T16908	T16904	punct	),construct
R11825	T16909	T16910	nsubj	that,lacks
R11826	T16910	T16904	ccomp	lacks,construct
R11827	T16911	T16913	det	the,domain
R11828	T16912	T16913	compound	HMG,domain
R11829	T16913	T16915	nsubjpass	domain,generated
R11830	T16914	T16915	auxpass	was,generated
R11831	T16915	T16910	ccomp	generated,lacks
R11832	T16916	T16915	punct	",",generated
R11833	T16917	T16918	mark	as,described
R11834	T16918	T16915	advcl	described,generated
R11835	T16919	T16918	agent	by,described
R11836	T16920	T16919	pobj	others,by
R11837	T16921	T16923	nmod	[,]
R11838	T16922	T16923	nummod	32,]
R11839	T16923	T16920	appos	],others
R11840	T16924	T16904	punct	.,construct
R11841	T16925	T16936	nsubjpass	Mutagenesis,done
R11842	T16926	T16925	prep	of,Mutagenesis
R11843	T16927	T16928	compound	Sox/Sox6,consensus
R11844	T16928	T16930	nmod	consensus,sites
R11845	T16929	T16930	amod	binding,sites
R11846	T16930	T16926	pobj	sites,of
R11847	T16931	T16930	prep	of,sites
R11848	T16932	T16934	det	the,promoter
R11849	T16933	T16934	amod	ɛy,promoter
R11850	T16934	T16931	pobj	promoter,of
R11851	T16935	T16936	auxpass	were,done
R11852	T16936	T16936	ROOT	done,done
R11853	T16937	T16936	agent	by,done
R11854	T16938	T16937	pobj	PCR,by
R11855	T16939	T16936	punct	.,done
R11856	T16940	T16941	amod	Forward,primers
R11857	T16941	T16951	nsubj	primers,include
R11858	T16942	T16941	acl	used,primers
R11859	T16943	T16944	aux	to,generate
R11860	T16944	T16942	xcomp	generate,used
R11861	T16945	T16950	det	these,constructs
R11862	T16946	T16950	amod	mutagenized,constructs
R11863	T16947	T16950	compound	ɛy,constructs
R11864	T16948	T16949	compound	promoter,reporter
R11865	T16949	T16950	compound	reporter,constructs
R11866	T16950	T16951	nsubj	constructs,include
R11867	T16951	T16951	ROOT	include,include
R11868	T16952	T16951	punct	:,include
R11869	T16953	T16967	nummod	MHB1661,MHB1662
R11870	T16954	T16956	punct	",",′
R11871	T16955	T16956	nummod	5,′
R11872	T16956	T16967	nmod	′,MHB1662
R11873	T16957	T16958	nummod	CCGCTCGAGAATGCAGTGCCAAGGGTCAGAACATTGTCTGCGAAG3,′
R11874	T16958	T16956	appos	′,′
R11875	T16959	T16960	punct	(,−
R11876	T16960	T16958	appos	−,′
R13910	T19582	T19581	prep	from,purchased

bionlp-st-ge-2016-test-tees

Id	Subject	Object	Predicate	Lexical cue
T20377	9885-9903	Protein	denotes	chromatin-antibody
T20376	9798-9801	Protein	denotes	IgG
T20375	9756-9760	Protein	denotes	Sox6
T19687	8575-8579	Protein	denotes	Sox6
T19686	8567-8569	Protein	denotes	HA
T19685	8560-8565	Protein	denotes	c-Myc
T19684	7812-7816	Protein	denotes	Sox6
T19683	7691-7715	Protein	denotes	T4 polynucleotide kinase
T19515	7485-7504	Protein	denotes	rabbit IgG antibody
T19514	7432-7446	Protein	denotes	c-Myc antibody
T19513	7389-7393	Protein	denotes	Sox6
T19512	7149-7153	Protein	denotes	Sox6
T19254	7072-7092	Protein	denotes	Sox6 coding sequence
T19253	6864-6866	Protein	denotes	HA
T19252	6854-6859	Protein	denotes	c-Myc
T19251	6797-6801	Protein	denotes	Sox6
T19250	6666-6670	Protein	denotes	Sox6
T17954	4145-4152	Protein	denotes	SPOT RT
T17953	3673-3677	Protein	denotes	Sox6
T17952	3592-3641	Protein	denotes	murine ɛy globin nucleotides 509–584; βmaj globin
T17418	2765-2780	Protein	denotes	βmaj/min globin
T17417	2675-2685	Protein	denotes	βH1 globin
T17416	2596-2603	Protein	denotes	MHB1668
T17415	2586-2594	Protein	denotes	ζ globin
T17414	2506-2513	Protein	denotes	MHB1666
T17413	2495-2504	Protein	denotes	ɛy globin
T17412	2366-2378	Protein	denotes	globin genes
T17411	2272-2283	Protein	denotes	globin mRNA
T17423	3471-3476	Protein	denotes	GAPDH
T17422	3168-3178	Protein	denotes	GAPDH mRNA
T17421	2904-2907	Protein	denotes	Rad
T17420	2900-2903	Protein	denotes	Bio
T17419	2895-2898	Protein	denotes	ROX
T16499	823-827	Protein	denotes	SstI
T16498	632-641	Gene_expression	denotes	contained
T16497	632-641	Gene_expression	denotes	contained
T16496	757-761	Protein	denotes	pGL3
T16495	730-753	Protein	denotes	firefly luciferase gene
T16494	651-664	Protein	denotes	HindIII sites
T16493	642-646	Protein	denotes	XhoI
T16492	404-417	Protein	denotes	ɛ globin gene
T16491	339-344	Protein	denotes	EcoRI
T16490	193-202	Protein	denotes	ɛy globin
T16489	137-157	Protein	denotes	ɛy proximal promoter
T18895	6435-6439	Protein	denotes	Sox6
T18894	6277-6284	Protein	denotes	FuGENE6
T18303	4891-4896	Protein	denotes	eosin
T18302	4875-4886	Protein	denotes	hematoxylin
T18569	5348-5352	Protein	denotes	Sox6
T16520	1975-2018	Protein	denotes	mutagenized ɛy promoter reporter constructs
T16519	1870-1874	Protein	denotes	Sox6
T16518	1866-1869	Protein	denotes	Sox
T16517	1786-1791	Negative_regulation	denotes	lacks
T16516	1796-1806	Protein	denotes	HMG domain
T16515	1761-1765	Protein	denotes	Sox6
T16514	1730-1734	Protein	denotes	Sox6
T16513	1685-1696	Positive_regulation	denotes	overexpress
T16512	1685-1696	Positive_regulation	denotes	overexpress
T16511	1685-1696	Gene_expression	denotes	overexpress
T16510	1685-1696	Gene_expression	denotes	overexpress
T16509	1697-1701	Protein	denotes	Sox6
T16508	1654-1658	Protein	denotes	Sox6
T16507	1601-1608	Protein	denotes	MHB1477
T16506	1587-1599	Protein	denotes	HindIII site
T16505	1461-1468	Protein	denotes	MHB1507
T16504	1148-1157	Protein	denotes	XhoI site
T16503	1006-1016	Gene_expression	denotes	expression
T16502	995-1005	Protein	denotes	luciferase
T16501	845-854	Protein	denotes	μLCRβ 9.3
T16500	828-832	Protein	denotes	XhoI
R11461	T16493	T16497	themeOf	XhoI,contained
R11462	T16494	T16498	themeOf	HindIII sites,contained
R11463	T16502	T16503	themeOf	luciferase,expression
R11464	T16508	T16510	themeOf	Sox6,overexpress
R11465	T16509	T16511	themeOf	Sox6,overexpress
R11466	T16510	T16512	themeOf	overexpress,overexpress
R11467	T16511	T16513	themeOf	overexpress,overexpress
R11468	T16516	T16517	themeOf	HMG domain,lacks

testone

Id	Subject	Object	Predicate	Lexical cue
T20330	10215-10217	Protein	denotes	ɛy
T20329	10110-10112	Protein	denotes	ɛy
T20328	9756-9760	Protein	denotes	Sox6
T20327	9585-9594	Protein	denotes	protein A
T19635	8575-8579	Protein	denotes	Sox6
T19634	8567-8569	Protein	denotes	HA
T19633	8560-8565	Protein	denotes	c-Myc
T19632	7908-7911	Protein	denotes	BSA
T19631	7812-7816	Protein	denotes	Sox6
T19630	7691-7715	Protein	denotes	T4 polynucleotide kinase
T19108	7072-7076	Protein	denotes	Sox6
T19107	6864-6866	Protein	denotes	HA
T19106	6854-6859	Protein	denotes	c-Myc
T19105	6797-6801	Protein	denotes	Sox6
T19104	6666-6670	Protein	denotes	Sox6
T19395	7432-7437	Protein	denotes	c-Myc
T19394	7389-7393	Protein	denotes	Sox6
T19393	7149-7153	Protein	denotes	Sox6
T18697	6435-6439	Protein	denotes	Sox6
T18696	6360-6362	Protein	denotes	ɛy
T18447	5348-5352	Protein	denotes	Sox6
T17735	3673-3677	Protein	denotes	Sox6
T17734	3630-3641	Protein	denotes	βmaj globin
T17733	3599-3608	Protein	denotes	ɛy globin
T17127	2256-2268	Negative_regulation	denotes	Quantitation
T17126	3471-3476	Protein	denotes	GAPDH
T17125	3168-3173	Protein	denotes	GAPDH
T17124	2770-2780	Protein	denotes	min globin
T17123	2765-2769	Protein	denotes	βmaj
T17122	2675-2685	Protein	denotes	βH1 globin
T17121	2586-2594	Protein	denotes	ζ globin
T17120	2495-2504	Protein	denotes	ɛy globin
T15960	1020-1026	Positive_regulation	denotes	driven
T15959	1006-1016	Gene_expression	denotes	expression
T15958	301-310	Negative_regulation	denotes	silencing
T15957	1906-1908	Protein	denotes	ɛy
T15956	1761-1765	Protein	denotes	Sox6
T15955	1730-1734	Protein	denotes	Sox6
T15954	1697-1701	Protein	denotes	Sox6
T15953	1654-1658	Protein	denotes	Sox6
T15952	1086-1088	Protein	denotes	ɛy
T15951	1034-1036	Protein	denotes	ɛy
T15950	995-1005	Protein	denotes	luciferase
T15949	907-909	Protein	denotes	ɛy
T15948	738-748	Protein	denotes	luciferase
T15947	693-695	Protein	denotes	ɛy
T15946	404-412	Protein	denotes	ɛ globin
T15945	294-295	Protein	denotes	ɛ
T15944	193-202	Protein	denotes	ɛy globin
T15943	137-139	Protein	denotes	ɛy
T15942	49-51	Protein	denotes	ɛy
R11066	T15945	T15958	themeOf	ɛ,silencing
R11067	T15950	T15959	themeOf	luciferase,expression
R11068	T15959	T15960	themeOf	expression,driven

test3

Id	Subject	Object	Predicate	Lexical cue
T20338	10215-10217	Protein	denotes	ɛy
T20337	10110-10112	Protein	denotes	ɛy
T20336	9756-9760	Protein	denotes	Sox6
T20335	9585-9594	Protein	denotes	protein A
T20334	10215-10217	Protein	denotes	ɛy
T20333	10110-10112	Protein	denotes	ɛy
T20332	9756-9760	Protein	denotes	Sox6
T20331	9585-9594	Protein	denotes	protein A
T19647	8575-8579	Protein	denotes	Sox6
T19646	8567-8569	Protein	denotes	HA
T19645	8560-8565	Protein	denotes	c-Myc
T19644	7908-7911	Protein	denotes	BSA
T19643	7812-7816	Protein	denotes	Sox6
T19642	7691-7715	Protein	denotes	T4 polynucleotide kinase
T19641	8575-8579	Protein	denotes	Sox6
T19640	8567-8569	Protein	denotes	HA
T19639	8560-8565	Protein	denotes	c-Myc
T19638	7908-7911	Protein	denotes	BSA
T19637	7812-7816	Protein	denotes	Sox6
T19636	7691-7715	Protein	denotes	T4 polynucleotide kinase
T19118	7072-7076	Protein	denotes	Sox6
T19117	6864-6866	Protein	denotes	HA
T19116	6854-6859	Protein	denotes	c-Myc
T19115	6797-6801	Protein	denotes	Sox6
T19114	6666-6670	Protein	denotes	Sox6
T19113	7072-7076	Protein	denotes	Sox6
T19112	6864-6866	Protein	denotes	HA
T19111	6854-6859	Protein	denotes	c-Myc
T19110	6797-6801	Protein	denotes	Sox6
T19109	6666-6670	Protein	denotes	Sox6
T19401	7432-7437	Protein	denotes	c-Myc
T19400	7389-7393	Protein	denotes	Sox6
T19399	7149-7153	Protein	denotes	Sox6
T19398	7432-7437	Protein	denotes	c-Myc
T19397	7389-7393	Protein	denotes	Sox6
T19396	7149-7153	Protein	denotes	Sox6
T18701	6435-6439	Protein	denotes	Sox6
T18700	6360-6362	Protein	denotes	ɛy
T18699	6435-6439	Protein	denotes	Sox6
T18698	6360-6362	Protein	denotes	ɛy
T18449	5348-5352	Protein	denotes	Sox6
T18448	5348-5352	Protein	denotes	Sox6
T17741	3673-3677	Protein	denotes	Sox6
T17740	3630-3641	Protein	denotes	βmaj globin
T17739	3599-3608	Protein	denotes	ɛy globin
T17738	3673-3677	Protein	denotes	Sox6
T17737	3630-3641	Protein	denotes	βmaj globin
T17736	3599-3608	Protein	denotes	ɛy globin
T17141	3471-3476	Protein	denotes	GAPDH
T17140	3168-3173	Protein	denotes	GAPDH
T17139	2770-2780	Protein	denotes	min globin
T17138	2765-2769	Protein	denotes	βmaj
T17137	2675-2685	Protein	denotes	βH1 globin
T17136	2586-2594	Protein	denotes	ζ globin
T17135	2495-2504	Protein	denotes	ɛy globin
T17134	3471-3476	Protein	denotes	GAPDH
T17133	3168-3173	Protein	denotes	GAPDH
T17132	2770-2780	Protein	denotes	min globin
T17131	2765-2769	Protein	denotes	βmaj
T17130	2675-2685	Protein	denotes	βH1 globin
T17129	2586-2594	Protein	denotes	ζ globin
T17128	2495-2504	Protein	denotes	ɛy globin
T15994	1906-1908	Protein	denotes	ɛy
T15993	1761-1765	Protein	denotes	Sox6
T15992	1730-1734	Protein	denotes	Sox6
T15991	1697-1701	Protein	denotes	Sox6
T15990	1654-1658	Protein	denotes	Sox6
T15989	1086-1088	Protein	denotes	ɛy
T15988	1034-1036	Protein	denotes	ɛy
T15987	1020-1026	Positive_regulation	denotes	driven
T15986	1006-1016	Gene_expression	denotes	expression
T15985	995-1005	Protein	denotes	luciferase
T15984	907-909	Protein	denotes	ɛy
T15983	738-748	Protein	denotes	luciferase
T15982	693-695	Protein	denotes	ɛy
T15981	404-412	Protein	denotes	ɛ globin
T15980	294-295	Protein	denotes	ɛ
T15979	193-202	Protein	denotes	ɛy globin
T15978	137-139	Protein	denotes	ɛy
T15977	49-51	Protein	denotes	ɛy
T15976	1906-1908	Protein	denotes	ɛy
T15975	1761-1765	Protein	denotes	Sox6
T15974	1730-1734	Protein	denotes	Sox6
T15973	1697-1701	Protein	denotes	Sox6
T15972	1654-1658	Protein	denotes	Sox6
T15971	1086-1088	Protein	denotes	ɛy
T15970	1034-1036	Protein	denotes	ɛy
T15969	995-1005	Protein	denotes	luciferase
T15968	907-909	Protein	denotes	ɛy
T15967	738-748	Protein	denotes	luciferase
T15966	693-695	Protein	denotes	ɛy
T15965	404-412	Protein	denotes	ɛ globin
T15964	294-295	Protein	denotes	ɛ
T15963	193-202	Protein	denotes	ɛy globin
T15962	137-139	Protein	denotes	ɛy
T15961	49-51	Protein	denotes	ɛy
R11069	T15985	T15986	themeOf	luciferase,expression
R11070	T15986	T15987	themeOf	expression,driven
R13575	T19116	T19115	themeOf	c-Myc,Sox6
R13576	T19117	T19115	themeOf	HA,Sox6
R13934	T19644	T19643	themeOf	BSA,Sox6

PMC:1359074 / 33632-44136 JSONTXT

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PMC:1359074 / 33632-44136 JSON TXT